Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004560

Physical Location & Seq

Genomic Coordinates Forward (+)

29893188 .. 29899109

Gene Structure 5922 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1169100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGAAAGATATAAAATTTTGGAAGAGCTTGGTGATGGCACTTGTGGTTGTGTATATAAGGCTCGTGATTTGAGAACAAAGGAGATTGTTGCTGTCAAGAAGATGAAGAGGAAGTTCTATTTCTGGGAGGAATACTGGAGGTTACGAGAGATTAAGGTACTTCGGAAACTAAATCATCCCAATATCATAAAGTTAAAGGAGGTTGTCAGGGAAAACAATGAGGTGTTCCTCATTTTTGAATATATGAACTATAATTTGTACCAAATAATGAAAGAACAAAGAAGACCCTTTTCAGAGGATGAGATTCGTAGCTTTATGTCTCAACTGCTGCATGGACTCAGTCACCTCCATAAAAGTGGGTATTTTCACCGAGATCTAAAGCCAGAGAATTTGCTGGTGACGAATGACGTTCTTAAAATTGCCGATTTTGGCTTGGCTCGAGAAGTATCATCGATGCCCCCTTACACTGAATATGTTTCCACACGCTGGTATCGAGCACCAGAAGTCTTGTTGCAGTCCAAACTATACACTCCTGCAGTTGACATGTGGGCAGCTGGCGCAATCCTAGCTGAGCTTTGCACTTCATCCCCTATTTTCCCTGGTGAAAGTGAGATAGATCAATTCTTCAAGATCTGCTGTGTGCTTGGTACTCCAGACTTGACTGTCTTTCCTGAAGGGACAAATGCCTCTCGATTATATAGCTTTATCAATTATGAAAAGATCTTGCCCGCTAACCTCTGCGATATCATTCCAAATGCAAGTCCAGAAGCTATTGATTTGATCATGCAACTATGTTCATGGGATTCCTCAAGGAGGCCAACCGCCGATGAATCATTGCAACATCCTTTTTTCCAGGTGGGCTGGATTCCTCGTTCACTTCCTGATTCACTTGACCTGAAGCTGAGTAACATGGGGGCAAAGCCAACACTCGAGTTGAAATTATCGGACTTTGGTGCTGAGCCTGAGGACTGCTTTCTTGGCTTGACGTTGGCTGTGAAACCCAGTGTTCCAGACTTCGATGTGGTCCATGATGTATCTCATCATATGAAAGAGGATGCATTATTTTGCTCTGGTTTGGAAGATCGTTCTGGACGATCTGTATTTTGGTCTCTGATGCCCCCCGACCAACGTGGAATTTGTGCCCCAGTAGAGCCTTCCTTTTCTTTATCATTCAGTTCAATTCAGCATCCATCCGTCAGAGTTCCACAATCGGCTGGTTTCTCCATCCCATCCTTGCAGCCGAACATCTTAGATGGTCGATTTTTGACCGTGTCTCCTCCCTTCCCACAAAGCCATTGCCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04110 ko04114 ko04115 ko04151 ko04218 ko04914 ko04934 ko05161 ko05162 ko05165 ko05168 ko05169 ko05200 ko05203 ko05215 ko05222 ko05226 map04068 map04110 map04114 map04115 map04151 map04218 map04914 map04934 map05161 map05162 map05165 map05168 map05169 map05200 map05203 map05215 map05222 map05226

Pfam Domains

Protein Families

Pkinase

Protein Analysis

435

Amino Acids

49.61

Weight (kDa)

5.83

Isoelectric Point (pI)

53.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 284	1e-73	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	5 - 223	9.3e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13020 AT4G13020 AT4G13020 AT4G13020 AT4G13020 AT4G13020 AT4G13020 AT4G13020
fragaria_vesca	FvH4_2g18351 FvH4_2g18351 FvH4_2g18351 FvH4_2g18351 FvH4_2g18351 FvH4_2g18351
malus_domestica	MD05G1169100.v1.1 MD10G1157400.v1.1
prunus_persica	Prupe.8G196200_v2.0.a1 Prupe.8G196200_v2.0.a1 Prupe.8G196200_v2.0.a1
pyrus_communis	pycom10g13590
rosa_chinensis	RchiOBHm_Chr6g0283731
rosa_laevigata	RLG00000012803
rosa_roxburghii	Rroxscaffold_7G00185230
rosa_rugosa	Rorug06G0157100 Rorug06G0157200 Rorug06G0157300
rosa_samantha	Rh6AG269300 Rh6BG271600 Rh6CG271600 Rh6DG265400
rosa_wichuraiana	Rw6G023290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	662
AccB7I	CCANNNNNTGG	1 cut(s)	486
AciI	CCGC	2 cut(s)	729, 822
AcsI	RAATTY	3 cut(s)	15, 388, 1134
AcuI	CTGAAG	2 cut(s)	693, 917
AfaI	GTAC	3 cut(s)	159, 260, 649
AfiI	CCNNNNNNNGG	2 cut(s)	486, 813
AflIII	ACRYGT	1 cut(s)	543
AgsI	TTSAA	4 cut(s)	239, 628, 937, 1179
AjnI	CCWGG	2 cut(s)	598, 852
AloI	GAACNNNNNNTCC	2 cut(s)	856, 888
AluBI	AGCT	8 cut(s)	28, 312, 554, 569, 574, 702, 770, 901
AluI	AGCT	8 cut(s)	28, 312, 554, 569, 574, 702, 770, 901
Alw21I	GWGCWC	1 cut(s)	499
Alw26I	GTCTC	3 cut(s)	324, 1113, 1278
Ama87I	CYCGRG	2 cut(s)	438, 929
AoxI	GGCC	1 cut(s)	815
ApeKI	GCWGC	3 cut(s)	328, 551, 1237
ApoI	RAATTY	3 cut(s)	15, 388, 1134
AspLEI	GCGC	1 cut(s)	560
AspS9I	GGNCC	1 cut(s)	1024
AsuHPI	GGTGA	5 cut(s)	44, 335, 359, 409, 614
AvaI	CYCGRG	2 cut(s)	438, 929
AvaII	GGWCC	1 cut(s)	1024
AxyI	CCTNAGG	1 cut(s)	963
BaeGI	GKGCMC	1 cut(s)	1144
BaeI	ACNNNNGTAYC	2 cut(s)	473, 506
BauI	CACGAG	1 cut(s)	63
BbsI	GAAGAC	1 cut(s)	289
Bbv12I	GWGCWC	1 cut(s)	499
BbvI	GCAGC	3 cut(s)	315, 563, 1249
BccI	CCATC	5 cut(s)	29, 1198, 1232, 1237, 1247
BciT130I	CCWGG	2 cut(s)	600, 854
BclI	TGATCA	1 cut(s)	780
BcoDI	GTCTC	3 cut(s)	324, 1113, 1278
BfaI	CTAG	1 cut(s)	566
BfmI	CTRYAG	1 cut(s)	534
BglII	AGATCT	3 cut(s)	373, 630, 720
BisI	GCNGC	3 cut(s)	329, 552, 1238
BlpI	GCTNAGC	2 cut(s)	570, 957
BlsI	GCNGC	3 cut(s)	330, 553, 1239
Bme1390I	CCNGG	2 cut(s)	600, 854
Bme18I	GGWCC	1 cut(s)	1024
BmeT110I	CYCGRG	2 cut(s)	438, 929
BmgT120I	GGNCC	1 cut(s)	1024
BmrFI	CCNGG	2 cut(s)	600, 854
BmrI	ACTGGG	2 cut(s)	996, 1139
BmsI	GCATC	4 cut(s)	444, 1045, 1104, 1195
BmuI	ACTGGG	2 cut(s)	996, 1139
BpiI	GAAGAC	1 cut(s)	289
BpmI	CTGGAG	2 cut(s)	157, 636
Bpu1102I	GCTNAGC	2 cut(s)	570, 957
BpuEI	CTTGAG	1 cut(s)	793
Bsa29I	ATCGAT	1 cut(s)	452
BsaI	GGTCTC	1 cut(s)	1113
BsaJI	CCNNGG	1 cut(s)	598
BsaXI	ACNNNNNCTCC	2 cut(s)	1258, 1288
Bsc4I	CCNNNNNNNGG	2 cut(s)	486, 813
Bse1I	ACTGG	3 cut(s)	140, 1002, 1145
Bse21I	CCTNAGG	1 cut(s)	963
Bse3DI	GCAATG	2 cut(s)	833, 1294
BseBI	CCWGG	2 cut(s)	600, 854
BseCI	ATCGAT	1 cut(s)	452
BseDI	CCNNGG	1 cut(s)	598
BseGI	GGATG	9 cut(s)	175, 304, 584, 841, 1060, 1186, 1190, 1224, 1229
BseLI	CCNNNNNNNGG	2 cut(s)	486, 813
BseMI	GCAATG	2 cut(s)	833, 1294
BseMII	CTCAG	5 cut(s)	352, 561, 893, 948, 954
BseNI	ACTGG	3 cut(s)	140, 1002, 1145
BseRI	GAGGAG	1 cut(s)	1266
BseSI	GKGCMC	1 cut(s)	1144
BseXI	GCAGC	3 cut(s)	315, 563, 1249
BshFI	GGCC	1 cut(s)	817
BshVI	ATCGAT	1 cut(s)	452
BsiHKAI	GWGCWC	1 cut(s)	499
BsiHKCI	CYCGRG	2 cut(s)	438, 929
BslFI	GGGAC	1 cut(s)	691
BslI	CCNNNNNNNGG	2 cut(s)	486, 813
BsmAI	GTCTC	3 cut(s)	324, 1113, 1278
BsmFI	GGGAC	1 cut(s)	691
BsnI	GGCC	1 cut(s)	817
Bso31I	GGTCTC	1 cut(s)	1113
BsoBI	CYCGRG	2 cut(s)	438, 929
Bsp1286I	GDGCHC	2 cut(s)	499, 1144
Bsp143I	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
Bsp1720I	GCTNAGC	2 cut(s)	570, 957
BspACI	CCGC	2 cut(s)	729, 822
BspANI	GGCC	1 cut(s)	817
BspCNI	CTCAG	5 cut(s)	351, 562, 894, 949, 955
BspDI	ATCGAT	1 cut(s)	452
BspMAI	CTGCAG	1 cut(s)	538
BspQI	GCTCTTC	1 cut(s)	18
BspTNI	GGTCTC	1 cut(s)	1113
BsrDI	GCAATG	2 cut(s)	833, 1294
BsrI	ACTGG	3 cut(s)	140, 1002, 1145
BssECI	CCNNGG	1 cut(s)	598
BssMI	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
BssSI	CACGAG	1 cut(s)	63
Bst2BI	CACGAG	1 cut(s)	63
Bst2UI	CCWGG	2 cut(s)	600, 854
Bst4CI	ACNGT	2 cut(s)	664, 1270
Bst6I	CTCTTC	2 cut(s)	18, 101
BstC8I	GCNNGC	2 cut(s)	556, 729
BstDEI	CTNAG	6 cut(s)	338, 570, 902, 957, 963, 1249
BstENI	CCTNNNNNAGG	1 cut(s)	811
BstF5I	GGATG	9 cut(s)	175, 304, 584, 841, 1060, 1186, 1190, 1224, 1229
BstHHI	GCGC	1 cut(s)	560
BstKTI	GATC	7 cut(s)	376, 619, 633, 723, 783, 1084, 1097
BstMAI	GTCTC	3 cut(s)	324, 1113, 1278
BstMBI	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
BstMWI	GCNNNNNNNGC	3 cut(s)	557, 566, 978
BstNI	CCWGG	2 cut(s)	600, 854
BstNSI	RCATGY	1 cut(s)	547
BstSCI	CCNGG	2 cut(s)	598, 852
BstSFI	CTRYAG	1 cut(s)	534
BstSLI	GKGCMC	1 cut(s)	1144
BstV1I	GCAGC	3 cut(s)	315, 563, 1249
BstV2I	GAAGAC	1 cut(s)	289
BstX2I	RGATCY	3 cut(s)	373, 630, 720
BstXI	CCANNNNNNTGG	1 cut(s)	356
BstYI	RGATCY	3 cut(s)	373, 630, 720
Bsu15I	ATCGAT	1 cut(s)	452
Bsu36I	CCTNAGG	1 cut(s)	963
BsuRI	GGCC	1 cut(s)	817
BsuTUI	ATCGAT	1 cut(s)	452
BtsCI	GGATG	9 cut(s)	175, 304, 584, 841, 1060, 1186, 1190, 1224, 1229
BtsIMutI	CAGTG	2 cut(s)	465, 1009
Cac8I	GCNNGC	2 cut(s)	556, 729
CfoI	GCGC	1 cut(s)	560
Cfr13I	GGNCC	1 cut(s)	1024
ClaI	ATCGAT	1 cut(s)	452
Csp6I	GTAC	3 cut(s)	158, 259, 648
CviAII	CATG	6 cut(s)	332, 544, 784, 798, 910, 1028
CviQI	GTAC	3 cut(s)	158, 259, 648
DdeI	CTNAG	6 cut(s)	338, 570, 902, 957, 963, 1249
DpnI	GATC	7 cut(s)	375, 618, 632, 722, 782, 1083, 1096
DpnII	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
DrdI	GACNNNNNNGTC	1 cut(s)	662
DseDI	GACNNNNNNGTC	1 cut(s)	662
Eam1104I	CTCTTC	2 cut(s)	18, 101
EarI	CTCTTC	2 cut(s)	18, 101
Eco31I	GGTCTC	1 cut(s)	1113
Eco32I	GATATC	1 cut(s)	745
Eco47I	GGWCC	1 cut(s)	1024
Eco57I	CTGAAG	2 cut(s)	693, 917
Eco81I	CCTNAGG	1 cut(s)	963
Eco88I	CYCGRG	2 cut(s)	438, 929
EcoNI	CCTNNNNNAGG	1 cut(s)	811
EcoRII	CCWGG	2 cut(s)	598, 852
EcoRV	GATATC	1 cut(s)	745
EcoT22I	ATGCAT	1 cut(s)	1060
FaeI	CATG	6 cut(s)	335, 547, 787, 801, 913, 1031
FaqI	GGGAC	1 cut(s)	691
FatI	CATG	6 cut(s)	331, 543, 783, 797, 909, 1027
FauI	CCCGC	1 cut(s)	736
FauNDI	CATATG	1 cut(s)	1044
FbaI	TGATCA	1 cut(s)	780
Fnu4HI	GCNGC	3 cut(s)	329, 552, 1238
FokI	GGATG	9 cut(s)	162, 311, 571, 828, 1067, 1173, 1177, 1211, 1216
Fsp4HI	GCNGC	3 cut(s)	329, 552, 1238
FspBI	CTAG	1 cut(s)	566
GlaI	GCGC	1 cut(s)	559
GluI	GCNGC	3 cut(s)	329, 552, 1238
GsuI	CTGGAG	2 cut(s)	157, 636
HaeIII	GGCC	1 cut(s)	817
HhaI	GCGC	1 cut(s)	560
Hin1II	CATG	6 cut(s)	335, 547, 787, 801, 913, 1031
Hin6I	GCGC	1 cut(s)	558
HinP1I	GCGC	1 cut(s)	558
HincII	GTYRAC	1 cut(s)	541
HindII	GTYRAC	1 cut(s)	541
HinfI	GANTC	6 cut(s)	304, 336, 803, 830, 865, 884
HphI	GGTGA	5 cut(s)	44, 335, 359, 409, 614
Hpy166II	GTNNAC	2 cut(s)	541, 875
Hpy188I	TCNGA	5 cut(s)	165, 295, 946, 1113, 1199
Hpy8I	GTNNAC	2 cut(s)	541, 875
HpyAV	CCTTC	3 cut(s)	668, 1164, 1291
HpyCH4III	ACNGT	2 cut(s)	664, 1270
HpyCH4IV	ACGT	3 cut(s)	408, 986, 1129
HpyCH4V	TGCA	9 cut(s)	331, 514, 536, 579, 758, 787, 838, 1058, 1237
HpyF10VI	GCNNNNNNNGC	3 cut(s)	557, 566, 978
HpyF3I	CTNAG	6 cut(s)	338, 570, 902, 957, 963, 1249
HpySE526I	ACGT	3 cut(s)	408, 986, 1129
Hsp92II	CATG	6 cut(s)	335, 547, 787, 801, 913, 1031
HspAI	GCGC	1 cut(s)	558
Ksp22I	TGATCA	1 cut(s)	780
Kzo9I	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
LguI	GCTCTTC	1 cut(s)	18
Lsp1109I	GCAGC	3 cut(s)	315, 563, 1249
LweI	GCATC	4 cut(s)	444, 1045, 1104, 1195
MaeI	CTAG	1 cut(s)	566
MaeII	ACGT	3 cut(s)	408, 986, 1129
MaeIII	GTNAC	4 cut(s)	141, 341, 397, 905
MalI	GATC	7 cut(s)	375, 618, 632, 722, 782, 1083, 1096
MboI	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
MboII	GAAGA	6 cut(s)	35, 112, 118, 294, 616, 1091
MflI	RGATCY	3 cut(s)	373, 630, 720
MhlI	GDGCHC	2 cut(s)	499, 1144
MluCI	AATT	9 cut(s)	15, 253, 388, 417, 620, 709, 938, 1134, 1179
MlyI	GAGTC	1 cut(s)	330
Mph1103I	ATGCAT	1 cut(s)	1060
MseI	TTAA	4 cut(s)	153, 194, 414, 1303
MslI	CAYNNNNRTG	1 cut(s)	354
MspA1I	CMGCKG	1 cut(s)	554
MspR9I	CCNGG	2 cut(s)	600, 854
MvaI	CCWGG	2 cut(s)	600, 854
MwoI	GCNNNNNNNGC	3 cut(s)	557, 566, 978
NdeI	CATATG	1 cut(s)	1044
NdeII	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
NlaIII	CATG	6 cut(s)	335, 547, 787, 801, 913, 1031
NmuCI	GTSAC	2 cut(s)	341, 397
NsiI	ATGCAT	1 cut(s)	1060
NspI	RCATGY	1 cut(s)	547
PaeR7I	CTCGAG	2 cut(s)	438, 929
PciI	ACATGT	1 cut(s)	543
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	2 cut(s)	490, 1090
PfeI	GAWTC	5 cut(s)	304, 803, 830, 865, 884
PflFI	GACNNNGTC	2 cut(s)	339, 1270
PflMI	CCANNNNNTGG	1 cut(s)	486
PkrI	GCNGC	3 cut(s)	330, 553, 1239
PleI	GAGTC	1 cut(s)	330
PpsI	GAGTC	1 cut(s)	330
PscI	ACATGT	1 cut(s)	543
Psp6I	CCWGG	2 cut(s)	598, 852
PspGI	CCWGG	2 cut(s)	598, 852
PspPI	GGNCC	1 cut(s)	1024
PspXI	VCTCGAGB	1 cut(s)	929
PstI	CTGCAG	1 cut(s)	538
PsuI	RGATCY	3 cut(s)	373, 630, 720
PsyI	GACNNNGTC	2 cut(s)	339, 1270
PvuII	CAGCTG	1 cut(s)	554
RsaI	GTAC	3 cut(s)	159, 260, 649
RsaNI	GTAC	3 cut(s)	158, 259, 648
RseI	CAYNNNNRTG	1 cut(s)	354
SapI	GCTCTTC	1 cut(s)	18
SaqAI	TTAA	4 cut(s)	153, 194, 414, 1303
SatI	GCNGC	3 cut(s)	329, 552, 1238
Sau3AI	GATC	7 cut(s)	373, 616, 630, 720, 780, 1081, 1094
Sau96I	GGNCC	1 cut(s)	1024
SchI	GAGTC	1 cut(s)	330
ScrFI	CCNGG	2 cut(s)	600, 854
SduI	GDGCHC	2 cut(s)	499, 1144
SfaNI	GCATC	4 cut(s)	444, 1045, 1104, 1195
SfcI	CTRYAG	1 cut(s)	534
Sfr274I	CTCGAG	2 cut(s)	438, 929
SinI	GGWCC	1 cut(s)	1024
SlaI	CTCGAG	2 cut(s)	438, 929
SmiMI	CAYNNNNRTG	1 cut(s)	354
SmlI	CTYRAG	3 cut(s)	438, 808, 929
SmoI	CTYRAG	3 cut(s)	438, 808, 929
Sse9I	AATT	9 cut(s)	15, 253, 388, 417, 620, 709, 938, 1134, 1179
SsiI	CCGC	2 cut(s)	729, 822
SspMI	CTAG	1 cut(s)	566
StyD4I	CCNGG	2 cut(s)	598, 852
TaaI	ACNGT	2 cut(s)	664, 1270
TaiI	ACGT	3 cut(s)	411, 989, 1132
TaqI	TCGA	7 cut(s)	439, 452, 493, 691, 930, 1017, 1258
TasI	AATT	9 cut(s)	15, 253, 388, 417, 620, 709, 938, 1134, 1179
TfiI	GAWTC	5 cut(s)	304, 803, 830, 865, 884
Tru1I	TTAA	4 cut(s)	153, 194, 414, 1303
Tru9I	TTAA	4 cut(s)	153, 194, 414, 1303
TscAI	CASTG	2 cut(s)	472, 1009
TseFI	GTSAC	2 cut(s)	341, 397
TseI	GCWGC	3 cut(s)	328, 551, 1237
Tsp45I	GTSAC	2 cut(s)	341, 397
TspDTI	ATGAA	8 cut(s)	119, 260, 284, 573, 729, 786, 843, 1061
TspGWI	ACGGA	1 cut(s)	1183
TspRI	CASTG	2 cut(s)	472, 1009
Tth111I	GACNNNGTC	2 cut(s)	339, 1270
Van91I	CCANNNNNTGG	1 cut(s)	486
VpaK11BI	GGWCC	1 cut(s)	1024
XagI	CCTNNNNNAGG	1 cut(s)	811
XapI	RAATTY	3 cut(s)	15, 388, 1134
XceI	RCATGY	1 cut(s)	547
XhoI	CTCGAG	2 cut(s)	438, 929
XspI	CTAG	1 cut(s)	566
Zsp2I	ATGCAT	1 cut(s)	1060

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.