MD05G1176500.v1.1

Desumoylating isopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008469
Physical Location & Seq
Forward (+)
30402686 .. 30405028
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1176500.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCAGAGGAGGGTCACAAGGTTATTCTAAATGTATATGACCTGAGCCAAGGATTAGCAAGGCAAATGTCCATGGCTTTCTTGGGGAAAGCCATTGAGGGCATATGGCACACAGGATTGGTGGTTTATGGTAATGAGTACTATTTTGGGGGAGGCATACAACATGCTCCTTCTGGGTCAACGCCATATGGGACACCAATTAAAGTGGTAGATTTGGGTGTGACACATGTGCCCAAGGATGTCTTTGAGATGTATTTGCAGGAGATCAGTCCTCGGTATACTGCTGAAACCTATAGTTTGCTTACTCACAATTGCAACAACTTTAGCAATGAGGTCGCTCAATTTTTGGTGGGTGCATCCATTCCGGACTATATTTTGCAGCTTCCTAATGAAGTTATGGGCAGTCCAATGGGAGCTCTTGTTATGCCCATGATACAGAATCTGGAAGCAACGTTGAAAAGTGGTGCTGCTCCTCAAGCTCCACAATTCCGGCCTGCTTCCGTGGTGCAACCAACAACTAATGCTGCGAAACCCATGAGTACTTCGAGCAAAGAGACTGGTGATAAGTCGAAATCTGGAGTGCAGCAGCTCACCAAGTCGGAAGAGCGGGGAGCATCAGGGAGTGCGGTTAAACCTCCCAACAGAGTTGTAGATCCTCTCGGGGATGCTCGCAGTAAGGTGCAAGAGGAGATCTCTGCTGAGTTTGCTGCAATCATGGCAAGTGGAACACTGCGTGCAAGCGAGGCTGCAGCCCTTGCAACGAGAAAAGTGATGCAGAGATATGGTCATGCAGGTTCCACGATGTCACAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

272

Amino Acids

28.94

Weight (kDa)

6.31

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 6 - 145 2.7e-49 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 782
AccBSI CCGCTC 1 cut(s) 607
AccI GTMKAC 1 cut(s) 278
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 2 cut(s) 607, 626
AclI AACGTT 1 cut(s) 452
AclWI GGATC 1 cut(s) 647
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 2 cut(s) 140, 541
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 1 cut(s) 457
AluBI AGCT 4 cut(s) 382, 416, 479, 589
AluI AGCT 4 cut(s) 382, 416, 479, 589
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 1 cut(s) 647
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 659
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 8 cut(s) 379, 467, 524, 583, 586, 707, 746, 749
AsuHPI GGTGA 2 cut(s) 572, 583
AvaI CYCGRG 1 cut(s) 659
BaeGI GKGCMC 1 cut(s) 234
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 8 cut(s) 391, 454, 511, 595, 598, 694, 733, 761
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BcoDI GTCTC 1 cut(s) 548
BfmI CTRYAG 2 cut(s) 292, 747
BfuAI ACCTGC 1 cut(s) 782
BglII AGATCT 1 cut(s) 690
BisI GCNGC 8 cut(s) 380, 468, 525, 584, 587, 708, 747, 750
BlsI GCNGC 8 cut(s) 381, 469, 526, 585, 588, 709, 748, 751
BmcAI AGTACT 2 cut(s) 140, 541
BmeT110I CYCGRG 1 cut(s) 659
BmiI GGNNCC 1 cut(s) 796
BmsI GCATC 4 cut(s) 365, 623, 655, 762
BplI GAGNNNNNCTC 2 cut(s) 677, 709
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 459
BsaJI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bse1I ACTGG 1 cut(s) 562
Bse3DI GCAATG 1 cut(s) 334
BseAI TCCGGA 1 cut(s) 364
BseDI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BseGI GGATG 3 cut(s) 244, 356, 670
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 2 cut(s) 35, 690
BseNI ACTGG 1 cut(s) 562
BseRI GAGGAG 3 cut(s) 23, 462, 701
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 8 cut(s) 391, 454, 511, 595, 598, 694, 733, 761
BsgI GTGCAG 1 cut(s) 602
BshFI GGCC 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 2 cut(s) 365, 490
BslFI GGGAC 1 cut(s) 205
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 234, 418
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 3 cut(s) 264, 652, 690
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 2 cut(s) 607, 626
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 2 cut(s) 36, 691
BspEI TCCGGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 796
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 1 cut(s) 782
BspPI GGATC 1 cut(s) 647
BspQI GCTCTTC 1 cut(s) 597
BsrBI CCGCTC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 334
BsrI ACTGG 1 cut(s) 562
BssECI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BssMI GATC 3 cut(s) 264, 652, 690
BssNAI GTATAC 1 cut(s) 279
BssT1I CCWWGG 3 cut(s) 49, 72, 234
Bst1107I GTATAC 1 cut(s) 279
Bst6I CTCTTC 1 cut(s) 597
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 4 cut(s) 495, 670, 735, 739
BstDEI CTNAG 2 cut(s) 44, 699
BstDSI CCRYGG 2 cut(s) 72, 501
BstF5I GGATG 3 cut(s) 244, 356, 670
BstKTI GATC 3 cut(s) 267, 655, 693
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 3 cut(s) 264, 652, 690
BstMWI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
BstNSI RCATGY 2 cut(s) 167, 230
BstSFI CTRYAG 2 cut(s) 292, 747
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 8 cut(s) 391, 454, 511, 595, 598, 694, 733, 761
BstX2I RGATCY 2 cut(s) 652, 690
BstYI RGATCY 2 cut(s) 652, 690
BstZ17I GTATAC 1 cut(s) 279
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 2 cut(s) 72, 501
BtsCI GGATG 3 cut(s) 244, 356, 670
BtsI GCAGTG 1 cut(s) 728
BtsIMutI CAGTG 1 cut(s) 728
BveI ACCTGC 1 cut(s) 782
Cac8I GCNNGC 4 cut(s) 495, 670, 735, 739
CaiI CAGNNNCTG 1 cut(s) 442
Csp6I GTAC 2 cut(s) 139, 540
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 7 cut(s) 73, 164, 227, 430, 535, 715, 788
CviQI GTAC 2 cut(s) 139, 540
DdeI CTNAG 2 cut(s) 44, 699
DpnI GATC 3 cut(s) 266, 654, 692
DpnII GATC 3 cut(s) 264, 652, 690
DraIII CACNNNGTG 1 cut(s) 734
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 3 cut(s) 49, 72, 234
Eco24I GRGCYC 1 cut(s) 418
Eco53kI GAGCTC 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 659
EcoICRI GAGCTC 1 cut(s) 416
EcoT14I CCWWGG 3 cut(s) 49, 72, 234
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 49, 72, 234
FaeI CATG 7 cut(s) 76, 167, 230, 433, 538, 718, 791
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 1 cut(s) 205
FatI CATG 7 cut(s) 72, 163, 226, 429, 534, 714, 787
FauI CCCGC 1 cut(s) 600
FauNDI CATATG 2 cut(s) 104, 187
FblI GTMKAC 1 cut(s) 278
Fnu4HI GCNGC 8 cut(s) 380, 468, 525, 584, 587, 708, 747, 750
FokI GGATG 3 cut(s) 251, 343, 677
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 8 cut(s) 380, 468, 525, 584, 587, 708, 747, 750
GluI GCNGC 8 cut(s) 380, 468, 525, 584, 587, 708, 747, 750
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 1 cut(s) 493
HapII CCGG 2 cut(s) 365, 490
Hin1II CATG 7 cut(s) 76, 167, 230, 433, 538, 718, 791
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 1 cut(s) 439
HpaII CCGG 2 cut(s) 365, 490
HphI GGTGA 2 cut(s) 572, 583
Hpy166II GTNNAC 2 cut(s) 180, 279
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 3 cut(s) 365, 443, 576
Hpy8I GTNNAC 2 cut(s) 180, 279
HpyAV CCTTC 1 cut(s) 180
HpyCH4IV ACGT 1 cut(s) 452
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
HpyF3I CTNAG 2 cut(s) 44, 699
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 7 cut(s) 76, 167, 230, 433, 538, 718, 791
Kpn2I TCCGGA 1 cut(s) 364
Kzo9I GATC 3 cut(s) 264, 652, 690
LguI GCTCTTC 1 cut(s) 597
LmnI GCTCC 5 cut(s) 172, 413, 475, 484, 611
Lsp1109I GCAGC 8 cut(s) 391, 454, 511, 595, 598, 694, 733, 761
LweI GCATC 4 cut(s) 365, 623, 655, 762
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 14, 220, 804
MalI GATC 3 cut(s) 266, 654, 692
MbiI CCGCTC 1 cut(s) 607
MboI GATC 3 cut(s) 264, 652, 690
MboII GAAGA 1 cut(s) 614
MfeI CAATTG 1 cut(s) 310
MflI RGATCY 2 cut(s) 652, 690
MhlI GDGCHC 2 cut(s) 234, 418
MluCI AATT 4 cut(s) 198, 310, 341, 485
MmeI TCCRAC 1 cut(s) 579
MroI TCCGGA 1 cut(s) 364
MseI TTAA 3 cut(s) 201, 630, 814
MspI CCGG 2 cut(s) 365, 490
MunI CAATTG 1 cut(s) 310
MwoI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 104, 187
NdeII GATC 3 cut(s) 264, 652, 690
NlaIII CATG 7 cut(s) 76, 167, 230, 433, 538, 718, 791
NlaIV GGNNCC 1 cut(s) 796
NmuCI GTSAC 3 cut(s) 14, 220, 804
NspI RCATGY 2 cut(s) 167, 230
PciI ACATGT 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 597
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 8 cut(s) 381, 469, 526, 585, 588, 709, 748, 751
PscI ACATGT 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 418
Psp1406I AACGTT 1 cut(s) 452
PspN4I GGNNCC 1 cut(s) 796
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 2 cut(s) 652, 690
RsaI GTAC 2 cut(s) 140, 541
RsaNI GTAC 2 cut(s) 139, 540
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 1 cut(s) 597
SaqAI TTAA 3 cut(s) 201, 630, 814
SatI GCNGC 8 cut(s) 380, 468, 525, 584, 587, 708, 747, 750
Sau3AI GATC 3 cut(s) 264, 652, 690
ScaI AGTACT 2 cut(s) 140, 541
SduI GDGCHC 2 cut(s) 234, 418
SfaNI GCATC 4 cut(s) 365, 623, 655, 762
SfcI CTRYAG 2 cut(s) 292, 747
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 4 cut(s) 198, 310, 341, 485
SsiI CCGC 2 cut(s) 607, 626
SstI GAGCTC 1 cut(s) 418
StyI CCWWGG 3 cut(s) 49, 72, 234
TaiI ACGT 1 cut(s) 455
TaqI TCGA 2 cut(s) 545, 569
TasI AATT 4 cut(s) 198, 310, 341, 485
TatI WGTACW 2 cut(s) 138, 539
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 3 cut(s) 201, 630, 814
Tru9I TTAA 3 cut(s) 201, 630, 814
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 3 cut(s) 14, 220, 804
TseI GCWGC 8 cut(s) 379, 467, 524, 583, 586, 707, 746, 749
Tsp45I GTSAC 3 cut(s) 14, 220, 804
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 1 cut(s) 735
XceI RCATGY 2 cut(s) 167, 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 278
ZrmI AGTACT 2 cut(s) 140, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.