MD10G1165200.v1.1

Desumoylating isopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008469
Physical Location & Seq
Forward (+)
25597324 .. 25599742
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1165200.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCAGAGGAGGTTCACAAGGTTACTTTGAATGTATACGACTTAAGCCAAGGATTAGCTAGGCAAATGTCCACGGCTTTCTTGGGGAAAGCCATCGAGGGCATATGGCACACGGGATTGGTGGTTTATGGTAATGAGTACTATTTTGGGGGAGGCATACAACATGCTCCTGCTGGGTCAACACCGTATGGGACACCAATTAAAGTGGTAGATTTGGGTGTCACACATGTGCCCAAGGATGTATTTGAGATGTATTTGCAGGAGATCAGTCCCCGGTATACTGCGGAAACCTATAGTTTACTTACTCACAATTGCAACAACTTTAGCAATGAGGTTGCTCAGTTTTTGGTGGGTGCAACCATTCCGGACTATATTTTGCAGCTTCCTAATGAAGTTATGGGCAGTCCAATGGGAGCTCTTATTATGCCCATGATACAGAATCTGGAAGCAACATTGAGAAGTGGTGCTGCTCCTCAAGCTCCACAGTTCAGGCCTACTTCCATGGTGCAAACGATGTCTAACACTGCGAAACCCTTGAGCACTTCCAACAAGGAGACTGGTGACAAATCGAAATCTGGAGCGCAGCAGGTCACCAAGTCAGAGGAGCGGGGAGCATCAGGAAGTGTGGTTAAACCTCCCAACGGAGTTGTAGATCCTCTCGGGGATGCTCGAAGTAAGGTGCAAGAGGAGATCTCTGCTGAGTTTGCTGCAATCATGGCAAGTGGAACACTGCGGGCAAGCGAGGCTGCAGCGCTTGCAACGAGAAGAGTGATGCAGAGATATGGTCATGCAGGTACCACGATGTCACAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

272

Amino Acids

29.0

Weight (kDa)

6.08

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 6 - 145 4.4e-49 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 577, 782
Acc65I GGTACC 1 cut(s) 794
AccB1I GGYRCC 1 cut(s) 794
AccBSI CCGCTC 1 cut(s) 607
AccI GTMKAC 2 cut(s) 36, 278
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 3 cut(s) 284, 607, 733
AclWI GGATC 1 cut(s) 647
AfaI GTAC 2 cut(s) 140, 796
AfeI AGCGCT 1 cut(s) 753
AfiI CCNNNNNNNGG 1 cut(s) 641
AflII CTTAAG 1 cut(s) 43
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 1 cut(s) 31
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 4 cut(s) 59, 382, 416, 479
AluI AGCT 4 cut(s) 59, 382, 416, 479
Alw21I GWGCWC 2 cut(s) 418, 542
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 1 cut(s) 647
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 659
Aor13HI TCCGGA 1 cut(s) 364
Aor51HI AGCGCT 1 cut(s) 753
AoxI GGCC 1 cut(s) 491
ApeKI GCWGC 6 cut(s) 379, 467, 583, 707, 746, 749
Asp718I GGTACC 1 cut(s) 794
AspLEI GCGC 2 cut(s) 583, 754
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 2 cut(s) 572, 583
AvaI CYCGRG 1 cut(s) 659
BaeGI GKGCMC 1 cut(s) 234
BanI GGYRCC 1 cut(s) 794
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 2 cut(s) 418, 542
BbvI GCAGC 6 cut(s) 391, 454, 595, 694, 733, 761
BccI CCATC 1 cut(s) 101
BceAI ACGGC 1 cut(s) 90
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 2 cut(s) 292, 747
BfoI RGCGCY 1 cut(s) 755
BfrI CTTAAG 1 cut(s) 43
BfuAI ACCTGC 2 cut(s) 577, 782
BglII AGATCT 1 cut(s) 690
BisI GCNGC 6 cut(s) 380, 468, 584, 708, 747, 750
BlsI GCNGC 6 cut(s) 381, 469, 585, 709, 748, 751
BmcAI AGTACT 1 cut(s) 140
Bme1390I CCNGG 1 cut(s) 274
BmeT110I CYCGRG 1 cut(s) 659
BmiI GGNNCC 1 cut(s) 796
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 3 cut(s) 623, 655, 762
BplI GAGNNNNNCTC 2 cut(s) 677, 709
BpmI CTGGAG 1 cut(s) 597
BpuEI CTTGAG 2 cut(s) 459, 556
BpuMI CCSGG 1 cut(s) 274
BsaJI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 562
Bse3DI GCAATG 1 cut(s) 334
BseAI TCCGGA 1 cut(s) 364
BseDI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BseGI GGATG 2 cut(s) 244, 670
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 2 cut(s) 353, 690
BseNI ACTGG 1 cut(s) 562
BseRI GAGGAG 4 cut(s) 23, 462, 617, 701
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 6 cut(s) 391, 454, 595, 694, 733, 761
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 493
BshNI GGYRCC 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 418, 542
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 2 cut(s) 274, 365
BslFI GGGAC 2 cut(s) 205, 255
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 2 cut(s) 205, 255
BsnI GGCC 1 cut(s) 493
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 3 cut(s) 234, 418, 542
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 3 cut(s) 264, 652, 690
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 3 cut(s) 284, 607, 733
BspANI GGCC 1 cut(s) 493
BspCNI CTCAG 2 cut(s) 352, 691
BspEI TCCGGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 796
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 2 cut(s) 577, 782
BspPI GGATC 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 794
BspTI CTTAAG 1 cut(s) 43
BsrBI CCGCTC 1 cut(s) 607
BsrDI GCAATG 1 cut(s) 334
BsrI ACTGG 1 cut(s) 562
BssECI CCNNGG 5 cut(s) 49, 72, 234, 272, 501
BssMI GATC 3 cut(s) 264, 652, 690
BssNAI GTATAC 2 cut(s) 37, 279
BssT1I CCWWGG 3 cut(s) 49, 234, 501
Bst1107I GTATAC 2 cut(s) 37, 279
Bst4CI ACNGT 2 cut(s) 186, 486
Bst6I CTCTTC 1 cut(s) 760
BstAFI CTTAAG 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 3 cut(s) 735, 739, 756
BstDEI CTNAG 2 cut(s) 339, 699
BstDSI CCRYGG 2 cut(s) 72, 501
BstEII GGTNACC 1 cut(s) 589
BstF5I GGATG 2 cut(s) 244, 670
BstH2I RGCGCY 1 cut(s) 755
BstHHI GCGC 2 cut(s) 583, 754
BstKTI GATC 3 cut(s) 267, 655, 693
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 3 cut(s) 264, 652, 690
BstMWI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
BstNSI RCATGY 2 cut(s) 167, 230
BstPI GGTNACC 1 cut(s) 589
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 2 cut(s) 292, 747
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 6 cut(s) 391, 454, 595, 694, 733, 761
BstX2I RGATCY 2 cut(s) 652, 690
BstYI RGATCY 2 cut(s) 652, 690
BstZ17I GTATAC 2 cut(s) 37, 279
BsuRI GGCC 1 cut(s) 493
BtgI CCRYGG 2 cut(s) 72, 501
BtsCI GGATG 2 cut(s) 244, 670
BtsI GCAGTG 2 cut(s) 522, 728
BtsIMutI CAGTG 2 cut(s) 522, 728
BveI ACCTGC 2 cut(s) 577, 782
Cac8I GCNNGC 3 cut(s) 735, 739, 756
CaiI CAGNNNCTG 1 cut(s) 442
CfoI GCGC 2 cut(s) 583, 754
Csp6I GTAC 2 cut(s) 139, 795
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 6 cut(s) 164, 227, 430, 502, 715, 788
CviJI RGCY 9 cut(s) 48, 59, 77, 92, 382, 416, 479, 493, 746
CviKI_1 RGCY 9 cut(s) 48, 59, 77, 92, 382, 416, 479, 493, 746
CviQI GTAC 2 cut(s) 139, 795
DdeI CTNAG 2 cut(s) 339, 699
DpnI GATC 3 cut(s) 266, 654, 692
DpnII GATC 3 cut(s) 264, 652, 690
Eam1104I CTCTTC 1 cut(s) 760
EarI CTCTTC 1 cut(s) 760
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 3 cut(s) 49, 234, 501
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 418
Eco47III AGCGCT 1 cut(s) 753
Eco53kI GAGCTC 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 416
EcoO65I GGTNACC 1 cut(s) 589
EcoT14I CCWWGG 3 cut(s) 49, 234, 501
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 49, 234, 501
FaeI CATG 6 cut(s) 167, 230, 433, 505, 718, 791
FaqI GGGAC 2 cut(s) 205, 255
FatI CATG 6 cut(s) 163, 226, 429, 501, 714, 787
FauI CCCGC 2 cut(s) 600, 726
FauNDI CATATG 1 cut(s) 104
FblI GTMKAC 2 cut(s) 36, 278
Fnu4HI GCNGC 6 cut(s) 380, 468, 584, 708, 747, 750
FokI GGATG 2 cut(s) 251, 677
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 6 cut(s) 380, 468, 584, 708, 747, 750
FspBI CTAG 1 cut(s) 60
GlaI GCGC 2 cut(s) 582, 753
GluI GCNGC 6 cut(s) 380, 468, 584, 708, 747, 750
GsaI CCCAGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 755
HaeIII GGCC 1 cut(s) 493
HapII CCGG 2 cut(s) 274, 365
HhaI GCGC 2 cut(s) 583, 754
Hin1II CATG 6 cut(s) 167, 230, 433, 505, 718, 791
Hin6I GCGC 2 cut(s) 581, 752
HinP1I GCGC 2 cut(s) 581, 752
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 1 cut(s) 439
HpaII CCGG 2 cut(s) 274, 365
HphI GGTGA 2 cut(s) 572, 583
Hpy166II GTNNAC 6 cut(s) 16, 37, 72, 180, 279, 299
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 4 cut(s) 365, 443, 576, 618
Hpy8I GTNNAC 6 cut(s) 16, 37, 72, 180, 279, 299
HpyCH4III ACNGT 2 cut(s) 186, 486
HpyF10VI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
HpyF3I CTNAG 2 cut(s) 339, 699
Hsp92II CATG 6 cut(s) 167, 230, 433, 505, 718, 791
HspAI GCGC 2 cut(s) 581, 752
Kpn2I TCCGGA 1 cut(s) 364
KpnI GGTACC 1 cut(s) 798
Kzo9I GATC 3 cut(s) 264, 652, 690
LmnI GCTCC 7 cut(s) 172, 413, 475, 484, 578, 604, 611
Lsp1109I GCAGC 6 cut(s) 391, 454, 595, 694, 733, 761
LweI GCATC 3 cut(s) 623, 655, 762
MaeI CTAG 1 cut(s) 60
MaeIII GTNAC 5 cut(s) 22, 220, 560, 589, 804
MalI GATC 3 cut(s) 266, 654, 692
MbiI CCGCTC 1 cut(s) 607
MboI GATC 3 cut(s) 264, 652, 690
MboII GAAGA 1 cut(s) 777
MfeI CAATTG 1 cut(s) 310
MflI RGATCY 2 cut(s) 652, 690
MhlI GDGCHC 3 cut(s) 234, 418, 542
MluCI AATT 2 cut(s) 198, 310
MmeI TCCRAC 1 cut(s) 570
MroI TCCGGA 1 cut(s) 364
MseI TTAA 4 cut(s) 44, 201, 630, 814
MslI CAYNNNNRTG 1 cut(s) 227
MspCI CTTAAG 1 cut(s) 43
MspI CCGG 2 cut(s) 274, 365
MspR9I CCNGG 1 cut(s) 274
MunI CAATTG 1 cut(s) 310
MwoI GCNNNNNNNGC 5 cut(s) 476, 704, 716, 743, 755
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 104
NdeII GATC 3 cut(s) 264, 652, 690
NlaIII CATG 6 cut(s) 167, 230, 433, 505, 718, 791
NlaIV GGNNCC 1 cut(s) 796
NmuCI GTSAC 4 cut(s) 220, 560, 589, 804
NspI RCATGY 2 cut(s) 167, 230
OliI CACNNNNGTG 1 cut(s) 227
PceI AGGCCT 1 cut(s) 493
PciI ACATGT 1 cut(s) 226
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 6 cut(s) 381, 469, 585, 709, 748, 751
PscI ACATGT 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 418
PspEI GGTNACC 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 796
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 2 cut(s) 652, 690
RsaI GTAC 2 cut(s) 140, 796
RsaNI GTAC 2 cut(s) 139, 795
RseI CAYNNNNRTG 1 cut(s) 227
SacI GAGCTC 1 cut(s) 418
SaqAI TTAA 4 cut(s) 44, 201, 630, 814
SatI GCNGC 6 cut(s) 380, 468, 584, 708, 747, 750
Sau3AI GATC 3 cut(s) 264, 652, 690
ScaI AGTACT 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 3 cut(s) 234, 418, 542
SfaNI GCATC 3 cut(s) 623, 655, 762
SfcI CTRYAG 2 cut(s) 292, 747
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 3 cut(s) 43, 474, 535
SmoI CTYRAG 3 cut(s) 43, 474, 535
Sse9I AATT 2 cut(s) 198, 310
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 3 cut(s) 284, 607, 733
SspMI CTAG 1 cut(s) 60
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 3 cut(s) 49, 234, 501
TaaI ACNGT 2 cut(s) 186, 486
TaqI TCGA 3 cut(s) 96, 569, 670
TasI AATT 2 cut(s) 198, 310
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 4 cut(s) 44, 201, 630, 814
Tru9I TTAA 4 cut(s) 44, 201, 630, 814
TscAI CASTG 2 cut(s) 529, 735
TseFI GTSAC 4 cut(s) 220, 560, 589, 804
TseI GCWGC 6 cut(s) 379, 467, 583, 707, 746, 749
Tsp45I GTSAC 4 cut(s) 220, 560, 589, 804
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 2 cut(s) 529, 735
Vha464I CTTAAG 1 cut(s) 43
XceI RCATGY 2 cut(s) 167, 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XmiI GTMKAC 2 cut(s) 36, 278
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.