Rw6G024140

Desumoylating isopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008469
Physical Location & Seq
Forward (+)
46967648 .. 46970031
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024140.1

Sequence Viewer

Length: 555 bp
ATGGCAGAGGAGGGTCATAGGGTTACTTTGAATGTGTATGATCTAAGCCAAGGTCTAGCTAGACAGATGTCTATGGCATTCATAGGGAAGGCCATTGAGGGCATATGGCATACAGGAGTCTCAGTTTACGGTAACGAGTACTATTTTGGGGGAGGCATACAACACGCTCCTGCTGGATCGACACCATATGGGACACCAATTCGGGTGGTAGAATTAGGTGAAACACATGTCCCCAAGGATGTTTTTGAGTCGTATTTACAGGAGATCAGTCCCCGATATACTGCTGAGACCTATAGTCTTCTAACTCACAATTGCAATAACTTTAGCAATGAGGTTGCTCAGTTTTTGGTTGGTGCAACCATACCCGACTATATTCTGCAGCTTCCTAATGAAATTATGAGCAGTCCAATGGGAGCTCTTATATTGCCTATGATTCAAAATTTGGAAATGACTTTGAAAAGTGGTGCTGCTCCTCAAGCTCCACAATTCAGGCCTCCTTCTGTAGTCCAGCCAACGGCTAATGTGACGAAACCTTTGGTAATTCTAACAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

184

Amino Acids

20.11

Weight (kDa)

5.26

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 6 - 145 3.1e-50 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 184
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 3 cut(s) 31, 437, 457
AhdI GACNNNNNGTC 1 cut(s) 294
AluBI AGCT 4 cut(s) 59, 382, 416, 479
AluI AGCT 4 cut(s) 59, 382, 416, 479
Alw21I GWGCWC 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 124, 281
AlwI GGATC 1 cut(s) 184
AoxI GGCC 2 cut(s) 90, 491
ApeKI GCWGC 2 cut(s) 379, 467
ApoI RAATTY 1 cut(s) 439
ArsI GACNNNNNNTTYG 2 cut(s) 517, 549
AsuHPI GGTGA 1 cut(s) 230
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 391, 454
BceAI ACGGC 1 cut(s) 531
BcoDI GTCTC 2 cut(s) 124, 281
BfaI CTAG 2 cut(s) 56, 60
BfmI CTRYAG 3 cut(s) 292, 377, 501
BisI GCNGC 2 cut(s) 380, 468
BlsI GCNGC 2 cut(s) 381, 469
BmcAI AGTACT 1 cut(s) 140
BmeRI GACNNNNNGTC 1 cut(s) 294
BoxI GACNNNNGTC 1 cut(s) 67
BpiI GAAGAC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 49, 234
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 49, 234
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 3 cut(s) 135, 276, 353
BseRI GAGGAG 2 cut(s) 23, 462
BseXI GCAGC 2 cut(s) 391, 454
BshFI GGCC 2 cut(s) 92, 493
BsiHKAI GWGCWC 1 cut(s) 418
BslFI GGGAC 3 cut(s) 205, 215, 255
BslI CCNNNNNNNGG 1 cut(s) 514
BsmAI GTCTC 2 cut(s) 124, 281
BsmFI GGGAC 3 cut(s) 205, 215, 255
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 2 cut(s) 92, 493
Bso31I GGTCTC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 40, 176, 264
BspANI GGCC 2 cut(s) 92, 493
BspCNI CTCAG 3 cut(s) 134, 277, 352
BspMAI CTGCAG 1 cut(s) 381
BspPI GGATC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 334
BssECI CCNNGG 2 cut(s) 49, 234
BssMI GATC 3 cut(s) 40, 176, 264
BssT1I CCWWGG 2 cut(s) 49, 234
Bst4CI ACNGT 1 cut(s) 131
BstDEI CTNAG 4 cut(s) 44, 121, 285, 339
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 3 cut(s) 43, 179, 267
BstMAI GTCTC 2 cut(s) 124, 281
BstMBI GATC 3 cut(s) 40, 176, 264
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 230
BstPAI GACNNNNGTC 1 cut(s) 67
BstSFI CTRYAG 3 cut(s) 292, 377, 501
BstV1I GCAGC 2 cut(s) 391, 454
BstV2I GAAGAC 1 cut(s) 290
BsuRI GGCC 2 cut(s) 92, 493
BtsCI GGATG 1 cut(s) 244
Csp6I GTAC 1 cut(s) 139
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 1 cut(s) 227
CviJI RGCY 9 cut(s) 48, 59, 92, 382, 416, 479, 493, 511, 518
CviKI_1 RGCY 9 cut(s) 48, 59, 92, 382, 416, 479, 493, 511, 518
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 4 cut(s) 44, 121, 285, 339
DpnI GATC 3 cut(s) 42, 178, 266
DpnII GATC 3 cut(s) 40, 176, 264
DriI GACNNNNNGTC 1 cut(s) 294
Eam1105I GACNNNNNGTC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 49, 234
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 416
EcoT14I CCWWGG 2 cut(s) 49, 234
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 49, 234
FaeI CATG 1 cut(s) 230
FaqI GGGAC 3 cut(s) 205, 215, 255
FatI CATG 1 cut(s) 226
FauNDI CATATG 2 cut(s) 104, 187
Fnu4HI GCNGC 2 cut(s) 380, 468
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 380, 468
FspBI CTAG 2 cut(s) 56, 60
GluI GCNGC 2 cut(s) 380, 468
HaeIII GGCC 2 cut(s) 92, 493
Hin1II CATG 1 cut(s) 230
HinfI GANTC 3 cut(s) 117, 248, 433
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 127
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 2 cut(s) 82, 507
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4V TGCA 3 cut(s) 315, 356, 379
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 4 cut(s) 44, 121, 285, 339
Hsp92II CATG 1 cut(s) 230
Kzo9I GATC 3 cut(s) 40, 176, 264
LmnI GCTCC 4 cut(s) 172, 413, 475, 484
LpnPI CCDG 6 cut(s) 99, 159, 183, 245, 475, 521
Lsp1109I GCAGC 2 cut(s) 391, 454
MaeI CTAG 2 cut(s) 56, 60
MaeIII GTNAC 3 cut(s) 22, 131, 523
MalI GATC 3 cut(s) 42, 178, 266
MboI GATC 3 cut(s) 40, 176, 264
MboII GAAGA 1 cut(s) 290
MfeI CAATTG 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 7 cut(s) 198, 212, 310, 393, 439, 485, 540
MlyI GAGTC 2 cut(s) 126, 257
MnlI CCTC 6 cut(s) 4, 91, 146, 325, 483, 504
MunI CAATTG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeI CATATG 2 cut(s) 104, 187
NdeII GATC 3 cut(s) 40, 176, 264
NlaIII CATG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 523
NspI RCATGY 1 cut(s) 230
PceI AGGCCT 1 cut(s) 493
PciI ACATGT 1 cut(s) 226
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 2 cut(s) 381, 469
PleI GAGTC 2 cut(s) 125, 256
PpsI GAGTC 2 cut(s) 125, 256
PscI ACATGT 1 cut(s) 226
PshAI GACNNNNGTC 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 381
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SacI GAGCTC 1 cut(s) 418
SatI GCNGC 2 cut(s) 380, 468
Sau3AI GATC 3 cut(s) 40, 176, 264
ScaI AGTACT 1 cut(s) 140
SchI GAGTC 2 cut(s) 126, 257
SduI GDGCHC 1 cut(s) 418
SetI ASST 9 cut(s) 55, 61, 220, 293, 336, 384, 418, 481, 535
SfcI CTRYAG 3 cut(s) 292, 377, 501
SmlI CTYRAG 1 cut(s) 474
SmoI CTYRAG 1 cut(s) 474
Sse9I AATT 7 cut(s) 198, 212, 310, 393, 439, 485, 540
SseBI AGGCCT 1 cut(s) 493
SspMI CTAG 2 cut(s) 56, 60
SstI GAGCTC 1 cut(s) 418
StuI AGGCCT 1 cut(s) 493
StyI CCWWGG 2 cut(s) 49, 234
TaaI ACNGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 179
TasI AATT 7 cut(s) 198, 212, 310, 393, 439, 485, 540
TatI WGTACW 1 cut(s) 138
TfiI GAWTC 1 cut(s) 433
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 379, 467
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 2 cut(s) 70, 405
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 230
XspI CTAG 2 cut(s) 56, 60
ZrmI AGTACT 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.