MD05G1182500.v1.1
TCP Family

Chaperonin CPN60-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004623
Physical Location & Seq
Reverse (-)
31120326 .. 31124784
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1182500.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGTATCGCTTCGCCACTAACCTTGCCTCCAAAGCCAGGTTGGCTAGGAACAGTTCTCAACAGATTGGCAGTAAATTGATTTCGAGAAGGAACTATGCAGCGAAAGAAATCAGATTCGGTGTTGAAGCCCGGGCCGGGATGCTTAGGGGTGTGGAAGAGCTCGCTGATGCTGTCAAAGTCACAATGGGGCCGAAAGGGCGCAATGTTGTTCTGGAACAAAGCTTTGGTGCCCCTAAAGTGACAAAGGATGGTGTCACTGTAGCAAAGAGTATTGAGTTCAAAGATAAAGTGAAGAACATTGGTGCTAGTCTTGTAAAGCAGGTCGCCAATGCTACAAACGATGCAGCAGGAGATGGTACCACTTGTGCTACAGTCCTTACTCGTGCTATATTTACTGAAGGATGCAAGTCAGTGGCGGCTGGAATGAATGCTATGGACCTAAGACGAGGCATTTCAATGGCAGTTGATTCTGTTGTTGCAAACTTGAAGAGTAGAGCTAGGATGATCAGCACATCTGAAGAAATAGCTCAGGTTGGTACAATATCAGCAAACGGAGAGAGAGAAATTGGTGAGCTCATAGCTAAAGCTATGGAGAAAGTTGGCAAAGAGGGTGTTATTACCATTTCTGATGGAAAAACACTGTATAATGAATTGGAGGTCGTTGAAGGAATGAAGCTGGATAGGGGCTACATATCCCCCTACTTCATCACCAATCCAAAGAACCAAAAATGTGAATTAGAAGATCCACTCGTCCTAATCCATGAGAAGAAACTCTCAAATTTAAATTCTATAGTTAAAATATTAGAGCTGGCCTTGCAGAAACAAAGACCTCTTGTAATTGTTGCTGAAGATGTGGAAAGTGAAGCACTTGCAACGCTCATTATAAACAAGCTTCGTGCTGGAATTAAGGTTTGTGCAATTAAAGCCCCTGGATTTGGAGAAAACAGGAAGGCAAATTTGCAGGACCTAGCTGTTCTTACGGGAGCCCAGGTAATAACTGAGGAGCTTGGTCTGAACATCGAAAAAGTGGGAATAGAAACACTGGGAACATGCAAAAGGATTACGGTATCAAAAGATGACACAGTCATTCTTGATGGGGCTGGTGACAAGAAAGCCATTGAAGAAAGATGTGAACAGCTGAGATCATCAATTGAATTGAGCACTTCTGATTATGACAAAGAGAAGTTACAAGAGCGCCTTGCAAAGCTTTCTGGTGGTGTTGCCGTCTTAAAGATTGGTGGAGCTAGCGAAGCGGAAGTTAGTGAAAAGAAGGATAGAGTAACTGATGCTCTAAACGCTACCAAGGCTGCTGTAGAGGAAGGAATTGTAGCCGGTGGTGGTGCCGCCCTTCTATATGCCTCAAAAGAGCTGGACAAGCTGGTGACTGCGAACTTTGATCAAAAGATCGGTGTTCAAATAATTCAGAATGCTCTAAAGATGCCTGTATCCACAATCGCTTCTAATGCTGGAGTCGAGGGGGCTGTTGTCGTTGGCAAACTACTAGAGCAGGACAATCCTGACCTTGGATACGATGCAGCTAAAGGTGAATATGTTGACATGATAAAGGCAGGGATCATCGACCCATTGAAAGTCATTAGAACCGCCTTGGTGGATGCTGCAAGTGTTTCCTCGTTGATGACCACTACCGAGGCAGTTGTGGTCGAGCTTCCGAAGGACGAGAAGGAAACCCCTGCAATGGGAGGAGGCATGGGAGGCATGGGAGGCATGGATTACTAG

Protein Analysis

579

Amino Acids

61.42

Weight (kDa)

6.01

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 54 - 554 1.4e-69 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
Acc36I ACCTGC 1 cut(s) 310
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 3 cut(s) 227, 356, 1340
AciI CCGC 4 cut(s) 416, 1253, 1344, 1602
AclWI GGATC 2 cut(s) 737, 1580
AcsI RAATTY 3 cut(s) 778, 784, 955
AcuI CTGAAG 3 cut(s) 417, 537, 867
AfaI GTAC 2 cut(s) 358, 538
AfiI CCNNNNNNNGG 6 cut(s) 36, 135, 935, 1523, 1696, 1697
AgsI TTSAA 9 cut(s) 125, 280, 456, 487, 665, 1121, 1154, 1415, 1588
AjnI CCWGG 3 cut(s) 35, 928, 987
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
Alw21I GWGCWC 3 cut(s) 162, 576, 1163
AlwI GGATC 2 cut(s) 737, 1580
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 3 cut(s) 132, 188, 810
ApeKI GCWGC 5 cut(s) 98, 344, 1307, 1535, 1616
ApoI RAATTY 3 cut(s) 778, 784, 955
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 201, 1197
AspS9I GGNCC 4 cut(s) 132, 188, 436, 964
AsuC2I CCSGG 3 cut(s) 130, 131, 136
AsuHPI GGTGA 5 cut(s) 581, 700, 1115, 1393, 1556
AsuNHI GCTAGC 1 cut(s) 1244
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 2 cut(s) 436, 964
BaeGI GKGCMC 1 cut(s) 232
BanI GGYRCC 3 cut(s) 227, 356, 1340
BanII GRGCYC 3 cut(s) 162, 576, 988
BauI CACGAG 1 cut(s) 381
Bbv12I GWGCWC 3 cut(s) 162, 576, 1163
BbvI GCAGC 5 cut(s) 110, 356, 1294, 1547, 1603
BccI CCATC 4 cut(s) 242, 347, 623, 1088
BceAI ACGGC 1 cut(s) 1208
BciT130I CCWGG 3 cut(s) 37, 930, 989
BciVI GTATCC 2 cut(s) 1456, 1520
BclI TGATCA 2 cut(s) 504, 1396
BcnI CCSGG 3 cut(s) 130, 131, 136
BfaI CTAG 7 cut(s) 45, 306, 498, 968, 1245, 1502, 1735
BfmI CTRYAG 4 cut(s) 258, 369, 789, 1311
BfoI RGCGCY 1 cut(s) 1198
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 2 cut(s) 1456, 1520
BglI GCCNNNNNGGC 2 cut(s) 41, 196
BisI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
BlsI GCNGC 7 cut(s) 100, 346, 418, 1309, 1345, 1537, 1618
Bme1390I CCNGG 6 cut(s) 37, 130, 131, 136, 930, 989
Bme18I GGWCC 2 cut(s) 436, 964
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 4 cut(s) 132, 188, 436, 964
BmiI GGNNCC 5 cut(s) 189, 229, 358, 985, 1342
BmrFI CCNGG 6 cut(s) 37, 130, 131, 136, 930, 989
BmrI ACTGGG 1 cut(s) 1052
BmsI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
BmtI GCTAGC 1 cut(s) 1248
BmuI ACTGGG 1 cut(s) 1052
BpmI CTGGAG 1 cut(s) 1488
Bpu10I CCTNAGC 2 cut(s) 143, 528
BpuMI CCSGG 3 cut(s) 130, 131, 136
BsaJI CCNNGG 7 cut(s) 129, 928, 987, 1302, 1522, 1605, 1647
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 975, 1005
Bsc4I CCNNNNNNNGG 6 cut(s) 36, 135, 935, 1523, 1696, 1697
Bse118I RCCGGY 1 cut(s) 1331
Bse1I ACTGG 1 cut(s) 1047
Bse3DI GCAATG 2 cut(s) 208, 1701
BseBI CCWGG 3 cut(s) 37, 930, 989
BseDI CCNNGG 7 cut(s) 129, 928, 987, 1302, 1522, 1605, 1647
BseGI GGATG 5 cut(s) 144, 253, 407, 507, 1618
BseLI CCNNNNNNNGG 6 cut(s) 36, 135, 935, 1523, 1696, 1697
BseMI GCAATG 2 cut(s) 208, 1701
BseMII CTCAG 3 cut(s) 542, 990, 1130
BseNI ACTGG 1 cut(s) 1047
BseRI GAGGAG 2 cut(s) 1016, 1716
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 5 cut(s) 110, 356, 1294, 1547, 1603
BshFI GGCC 3 cut(s) 134, 190, 812
BshNI GGYRCC 3 cut(s) 227, 356, 1340
BsiHKAI GWGCWC 3 cut(s) 162, 576, 1163
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 3 cut(s) 130, 135, 1332
BslI CCNNNNNNNGG 6 cut(s) 36, 135, 935, 1523, 1696, 1697
BsmI GAATGC 2 cut(s) 433, 1432
BsnI GGCC 3 cut(s) 134, 190, 812
BsoBI CYCGRG 1 cut(s) 129
Bsp1286I GDGCHC 5 cut(s) 162, 232, 576, 988, 1163
Bsp143I GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
BspACI CCGC 4 cut(s) 416, 1253, 1344, 1602
BspANI GGCC 3 cut(s) 134, 190, 812
BspCNI CTCAG 3 cut(s) 541, 991, 1131
BspLI GGNNCC 5 cut(s) 189, 229, 358, 985, 1342
BspMI ACCTGC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 1248
BspPI GGATC 2 cut(s) 737, 1580
BspQI GCTCTTC 1 cut(s) 150
BspT107I GGYRCC 3 cut(s) 227, 356, 1340
BsrDI GCAATG 2 cut(s) 208, 1701
BsrFI RCCGGY 1 cut(s) 1331
BsrI ACTGG 1 cut(s) 1047
BssAI RCCGGY 1 cut(s) 1331
BssECI CCNNGG 7 cut(s) 129, 928, 987, 1302, 1522, 1605, 1647
BssMI GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 3 cut(s) 1302, 1522, 1605
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 3 cut(s) 37, 930, 989
Bst4CI ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
Bst6I CTCTTC 2 cut(s) 150, 482
BstC8I GCNNGC 3 cut(s) 162, 810, 1246
BstDEI CTNAG 5 cut(s) 143, 440, 528, 999, 1139
BstF5I GGATG 5 cut(s) 144, 253, 407, 507, 1618
BstH2I RGCGCY 1 cut(s) 1198
BstHHI GCGC 2 cut(s) 201, 1197
BstKTI GATC 6 cut(s) 507, 745, 1145, 1399, 1407, 1575
BstMBI GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
BstNI CCWGG 3 cut(s) 37, 930, 989
BstNSI RCATGY 1 cut(s) 1053
BstSCI CCNGG 6 cut(s) 35, 128, 129, 134, 928, 987
BstSFI CTRYAG 4 cut(s) 258, 369, 789, 1311
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 5 cut(s) 110, 356, 1294, 1547, 1603
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuI GTATCC 2 cut(s) 1456, 1520
BsuRI GGCC 3 cut(s) 134, 190, 812
BtsCI GGATG 5 cut(s) 144, 253, 407, 507, 1618
BtsIMutI CAGTG 4 cut(s) 255, 417, 638, 1040
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 3 cut(s) 162, 810, 1246
CfoI GCGC 2 cut(s) 201, 1197
Cfr10I RCCGGY 1 cut(s) 1331
Cfr13I GGNCC 4 cut(s) 132, 188, 436, 964
Cfr9I CCCGGG 1 cut(s) 129
Csp6I GTAC 2 cut(s) 357, 537
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 6 cut(s) 761, 1050, 1558, 1708, 1717, 1726
CviQI GTAC 2 cut(s) 357, 537
DdeI CTNAG 5 cut(s) 143, 440, 528, 999, 1139
DpnI GATC 6 cut(s) 506, 744, 1144, 1398, 1406, 1574
DpnII GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
DraI TTTAAA 1 cut(s) 783
Eam1104I CTCTTC 2 cut(s) 150, 482
EarI CTCTTC 2 cut(s) 150, 482
Ecl136II GAGCTC 2 cut(s) 160, 574
Eco130I CCWWGG 3 cut(s) 1302, 1522, 1605
Eco24I GRGCYC 3 cut(s) 162, 576, 988
Eco47I GGWCC 2 cut(s) 436, 964
Eco53kI GAGCTC 2 cut(s) 160, 574
Eco57I CTGAAG 3 cut(s) 417, 537, 867
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 2 cut(s) 160, 574
EcoO109I RGGNCCY 1 cut(s) 964
EcoRII CCWGG 3 cut(s) 35, 928, 987
EcoT14I CCWWGG 3 cut(s) 1302, 1522, 1605
EcoT38I GRGCYC 3 cut(s) 162, 576, 988
ErhI CCWWGG 3 cut(s) 1302, 1522, 1605
FaeI CATG 6 cut(s) 764, 1053, 1561, 1711, 1720, 1729
FalI AAGNNNNNCTT 2 cut(s) 1182, 1214
FatI CATG 6 cut(s) 760, 1049, 1557, 1707, 1716, 1725
FbaI TGATCA 2 cut(s) 504, 1396
Fnu4HI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
FokI GGATG 5 cut(s) 151, 260, 414, 514, 1625
FriOI GRGCYC 3 cut(s) 162, 576, 988
Fsp4HI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
FspBI CTAG 7 cut(s) 45, 306, 498, 968, 1245, 1502, 1735
GlaI GCGC 2 cut(s) 200, 1196
GluI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
GsuI CTGGAG 1 cut(s) 1488
HaeII RGCGCY 1 cut(s) 1198
HaeIII GGCC 3 cut(s) 134, 190, 812
HapII CCGG 3 cut(s) 130, 135, 1332
HhaI GCGC 2 cut(s) 201, 1197
Hin1II CATG 6 cut(s) 764, 1053, 1561, 1711, 1720, 1729
Hin6I GCGC 2 cut(s) 199, 1195
HinP1I GCGC 2 cut(s) 199, 1195
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HindIII AAGCTT 3 cut(s) 220, 890, 1205
HinfI GANTC 3 cut(s) 114, 467, 1470
HpaII CCGG 3 cut(s) 130, 135, 1332
HphI GGTGA 5 cut(s) 581, 700, 1115, 1393, 1556
Hpy166II GTNNAC 2 cut(s) 1133, 1555
Hpy188I TCNGA 7 cut(s) 113, 517, 628, 1014, 1168, 1425, 1671
Hpy188III TCNNGA 4 cut(s) 84, 212, 1091, 1517
Hpy8I GTNNAC 2 cut(s) 1133, 1555
HpyAV CCTTC 9 cut(s) 81, 392, 659, 943, 1264, 1313, 1358, 1666, 1675
HpyCH4III ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
HpyF3I CTNAG 5 cut(s) 143, 440, 528, 999, 1139
Hsp92II CATG 6 cut(s) 764, 1053, 1561, 1711, 1720, 1729
HspAI GCGC 2 cut(s) 199, 1195
KpnI GGTACC 1 cut(s) 360
Ksp22I TGATCA 2 cut(s) 504, 1396
Kzo9I GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 3 cut(s) 983, 1003, 1241
Lsp1109I GCAGC 5 cut(s) 110, 356, 1294, 1547, 1603
LweI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
MaeI CTAG 7 cut(s) 45, 306, 498, 968, 1245, 1502, 1735
MaeIII GTNAC 7 cut(s) 178, 238, 253, 1103, 1185, 1279, 1381
MalI GATC 6 cut(s) 506, 744, 1144, 1398, 1406, 1574
MboI GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
MboII GAAGA 8 cut(s) 167, 304, 499, 530, 752, 778, 860, 1133
MfeI CAATTG 1 cut(s) 1149
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 5 cut(s) 162, 232, 576, 988, 1163
MlyI GAGTC 1 cut(s) 1479
MseI TTAA 5 cut(s) 782, 795, 906, 921, 1229
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 1138
MspI CCGG 3 cut(s) 130, 135, 1332
MspR9I CCNGG 6 cut(s) 37, 130, 131, 136, 930, 989
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 2 cut(s) 433, 1432
MvaI CCWGG 3 cut(s) 37, 930, 989
NciI CCSGG 3 cut(s) 130, 131, 136
NdeII GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
NheI GCTAGC 1 cut(s) 1244
NlaIII CATG 6 cut(s) 764, 1053, 1561, 1711, 1720, 1729
NlaIV GGNNCC 5 cut(s) 189, 229, 358, 985, 1342
NmuCI GTSAC 5 cut(s) 178, 238, 253, 1103, 1381
NspI RCATGY 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 2 cut(s) 433, 1432
PfeI GAWTC 2 cut(s) 114, 467
PflFI GACNNNGTC 1 cut(s) 1082
PkrI GCNGC 7 cut(s) 100, 346, 418, 1309, 1345, 1537, 1618
PleI GAGTC 1 cut(s) 1478
PpsI GAGTC 1 cut(s) 1478
PpuMI RGGWCCY 1 cut(s) 964
PsiI TTATAA 1 cut(s) 884
Psp124BI GAGCTC 2 cut(s) 162, 576
Psp5II RGGWCCY 1 cut(s) 964
Psp6I CCWGG 3 cut(s) 35, 928, 987
PspGI CCWGG 3 cut(s) 35, 928, 987
PspN4I GGNNCC 5 cut(s) 189, 229, 358, 985, 1342
PspPI GGNCC 4 cut(s) 132, 188, 436, 964
PspPPI RGGWCCY 1 cut(s) 964
PsuI RGATCY 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 1082
PvuII CAGCTG 1 cut(s) 1138
RsaI GTAC 2 cut(s) 358, 538
RsaNI GTAC 2 cut(s) 357, 537
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 2 cut(s) 162, 576
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 5 cut(s) 782, 795, 906, 921, 1229
SatI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
Sau3AI GATC 6 cut(s) 504, 742, 1142, 1396, 1404, 1572
Sau96I GGNCC 4 cut(s) 132, 188, 436, 964
SchI GAGTC 1 cut(s) 1479
ScrFI CCNGG 6 cut(s) 37, 130, 131, 136, 930, 989
SduI GDGCHC 5 cut(s) 162, 232, 576, 988, 1163
SfaNI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
SfcI CTRYAG 4 cut(s) 258, 369, 789, 1311
SinI GGWCC 2 cut(s) 436, 964
SmaI CCCGGG 1 cut(s) 131
SmiI ATTTAAAT 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 455
SrfI GCCCGGGC 1 cut(s) 131
SsiI CCGC 4 cut(s) 416, 1253, 1344, 1602
SspI AATATT 1 cut(s) 801
SspMI CTAG 7 cut(s) 45, 306, 498, 968, 1245, 1502, 1735
SstI GAGCTC 2 cut(s) 162, 576
StyD4I CCNGG 6 cut(s) 35, 128, 129, 134, 928, 987
StyI CCWWGG 3 cut(s) 1302, 1522, 1605
SwaI ATTTAAAT 1 cut(s) 783
TaaI ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
TaqI TCGA 5 cut(s) 83, 1020, 1473, 1578, 1662
TauI GCSGC 2 cut(s) 419, 1346
TfiI GAWTC 2 cut(s) 114, 467
Tru1I TTAA 5 cut(s) 782, 795, 906, 921, 1229
Tru9I TTAA 5 cut(s) 782, 795, 906, 921, 1229
TscAI CASTG 4 cut(s) 262, 417, 645, 1047
TseFI GTSAC 5 cut(s) 178, 238, 253, 1103, 1381
TseI GCWGC 5 cut(s) 98, 344, 1307, 1535, 1616
Tsp45I GTSAC 5 cut(s) 178, 238, 253, 1103, 1381
TspDTI ATGAA 4 cut(s) 440, 663, 686, 694
TspGWI ACGGA 1 cut(s) 567
TspMI CCCGGG 1 cut(s) 129
TspRI CASTG 4 cut(s) 262, 417, 645, 1047
Tth111I GACNNNGTC 1 cut(s) 1082
VpaK11BI GGWCC 2 cut(s) 436, 964
XapI RAATTY 3 cut(s) 778, 784, 955
XceI RCATGY 1 cut(s) 1053
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 129
XspI CTAG 7 cut(s) 45, 306, 498, 968, 1245, 1502, 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.