MD10G1170700.v1.1
TCP Family

Chaperonin CPN60-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004623
Physical Location & Seq
Reverse (-)
26339607 .. 26343937
4331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1170700.v1.1.491

Sequence Viewer

Length: 1728 bp
ATGTATCGCTTCGCCACTAACCTTGCCTCCAAAGCCAGGGTGGCTAGGAACAGTTCTCAACAGATTGGTAGTAAATTGATTTCGAGAAGGAACTATGCGGCGAAAGAAATCAGATTCGGTGTTGAAGCCCGGGCCGGGATGCTTAGGGGTGTGGAAGAGCTCGCTGATGCTGTTAAAGTCACAATGGGGCCAAAAGGACGCAATGTTGTTCTAGAACAAAGCTTTGGTGCCCCTAAAGTGACAAAGGATGGTGTCACTGTAGCAAAGAGTATTGAGTTCAAAGATAAAGTCAAGAACATTGGTGCTAGTCTTGTAAAGCAGGTTGCAAATGCTACAAATGATGCAGCAGGAGATGGTACCACTTGTGCTACAGTCCTTACCCGCGCTATATTTACTGAAGGATGCAAGTCAGTGGCGGCTGGAATGAATGCTATGGACCTAAGACGAGGCATTTCAATGGCAGTTGATTCCGTTGTTGCAAACTTGAAGAGTAGGGCTCGGATGATCAGCACATCTGAAGAAATAGCTCAGGTTGGTACAATATCAGCAAACGGAGAGAGGGAAATTGGTGAGCTAATAGCTAAGGCTATGGAGAAAGTTGGCAAAGAGGGTGTTATTACCATAGCTGATGGAAAAACACTGTATAACGAGTTGGAGGTCGTTGAAGGAATGAAGCTGGATAGGGGTTACATATCCCCTTATTTTATCACCAATCCGAAGAACCAAAAATGTGAATTAGAAGACCCACTCATCCTGATCCATGAGAAGAAACTCTCAAATCTAAATTCTATAGTGAAAATATTAGAGTTGGCCTTGCAGAAACAAAGACCTCTTGTGATTGTTGCGGAAGATGTGGAAAGTGAAGCTCTAGCGACACTCATTATAAACAAGCTTCGTGCTGGAATTAAGGTTTGTGCAATTAAAGCCCCTGGATTTGGAGAAAACAGGAAGGCAAATTTGCAGGACCTAGCCATTCTTACGGGAGCCCAGGTAATAACTGAAGAGCTTGGTCTGAACATCGAAAAAGTGGGAGTAGAAACACTTGGAACATGCAAAAGGATTACGGTATCAAAAGATGACACCGTCATACTTGATGGGGCTGGTGACAAGAAAGCCATTGAAGAAAGATGTGAACAGCTGAGATCATCAATTGAATTGAGCACTTCTGATTATGACAAAGAGAAGTTACAAGAGCGCCTTGCAAAGATTTCTGGTGGTGTTGCCGTCTTGAAGATTGGTGGAGCTAGCGAAGCAGAAGTTGGTGAAAAGAAGGATAGAGTAACTGATGCCCTAAACGCCACCAAGGCTGCTGTAGAGGAAGGAATTGTACCTGGTGGTGGTGCTGCCCTTCTTTATGCCTCAAAAGAGCTGGAAAAGTTGATGACTGCCAACTTCGATCAAAAGATTGGTGTTCAAATAATTCAGAATGCTCTCAAGATGCCTGTATCCACAATAGCTTCTAATGCCGGAGTCGAGGGGGCTGTTGTCGTTGGCAAACTATTGGAGCAGGACAATCCTGACCTTGGATATGATGCAGCGAAAGGTGAATATGTAGACATGATTAAGGCTGGAATCATTGATCCATTGAAAGTCATTAGAACCGCCTTGGTAGATGCTGCAAGTGTTTCCTCGTTGATGACCACTACTGAGGCTGTTGTTGTCGAGCTTCCCAAGGACGAGAAGGAAACCCCTGCAATGGGAGGAGGCATGGGAGGCATGGATTACTAG

Protein Analysis

576

Amino Acids

61.2

Weight (kDa)

6.01

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 54 - 554 1.2e-70 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
Acc36I ACCTGC 1 cut(s) 310
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 2 cut(s) 227, 356
AccI GTMKAC 1 cut(s) 1554
AccII CGCG 1 cut(s) 384
AciI CCGC 5 cut(s) 98, 382, 416, 845, 1602
AclWI GGATC 2 cut(s) 751, 1574
AcsI RAATTY 2 cut(s) 784, 955
AcuI CTGAAG 3 cut(s) 417, 537, 1020
AfaI GTAC 3 cut(s) 358, 538, 1329
AfiI CCNNNNNNNGG 7 cut(s) 36, 135, 935, 1337, 1523, 1696, 1697
AjnI CCWGG 4 cut(s) 35, 928, 987, 1330
AjuI GAANNNNNNNTTGG 4 cut(s) 207, 239, 1242, 1274
Alw21I GWGCWC 2 cut(s) 162, 1163
AlwI GGATC 2 cut(s) 751, 1574
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 3 cut(s) 132, 188, 810
ApeKI GCWGC 5 cut(s) 344, 1307, 1343, 1535, 1616
ApoI RAATTY 2 cut(s) 784, 955
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 386, 1197
AspS9I GGNCC 4 cut(s) 132, 188, 436, 964
AsuC2I CCSGG 3 cut(s) 130, 131, 136
AsuHPI GGTGA 5 cut(s) 581, 700, 1115, 1274, 1556
AsuNHI GCTAGC 1 cut(s) 1244
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 2 cut(s) 436, 964
BaeGI GKGCMC 1 cut(s) 232
BanI GGYRCC 2 cut(s) 227, 356
BanII GRGCYC 3 cut(s) 162, 499, 988
BarI GAAGNNNNNNTAC 2 cut(s) 1311, 1343
BbsI GAAGAC 1 cut(s) 747
Bbv12I GWGCWC 2 cut(s) 162, 1163
BbvI GCAGC 5 cut(s) 356, 1294, 1330, 1547, 1603
BccI CCATC 4 cut(s) 242, 347, 623, 1088
BceAI ACGGC 1 cut(s) 1208
BciT130I CCWGG 4 cut(s) 37, 930, 989, 1332
BciVI GTATCC 1 cut(s) 1456
BclI TGATCA 1 cut(s) 504
BcnI CCSGG 3 cut(s) 130, 131, 136
BfaI CTAG 7 cut(s) 45, 212, 306, 869, 968, 1245, 1726
BfmI CTRYAG 4 cut(s) 258, 369, 789, 1311
BfoI RGCGCY 1 cut(s) 1198
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 1456
BglI GCCNNNNNGGC 2 cut(s) 41, 1304
BisI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
BlsI GCNGC 7 cut(s) 100, 346, 418, 1309, 1345, 1537, 1618
Bme1390I CCNGG 7 cut(s) 37, 130, 131, 136, 930, 989, 1332
Bme18I GGWCC 2 cut(s) 436, 964
BmeT110I CYCGRG 1 cut(s) 129
BmgT120I GGNCC 4 cut(s) 132, 188, 436, 964
BmiI GGNNCC 4 cut(s) 189, 229, 358, 985
BmrFI CCNGG 7 cut(s) 37, 130, 131, 136, 930, 989, 1332
BmsI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
BmtI GCTAGC 1 cut(s) 1248
BpiI GAAGAC 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 481, 513
Bpu10I CCTNAGC 3 cut(s) 143, 528, 582
BpuEI CTTGAG 1 cut(s) 1418
BpuMI CCSGG 3 cut(s) 130, 131, 136
BsaJI CCNNGG 8 cut(s) 36, 129, 928, 987, 1302, 1522, 1605, 1671
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 975, 1005
Bsc4I CCNNNNNNNGG 7 cut(s) 36, 135, 935, 1337, 1523, 1696, 1697
Bse3DI GCAATG 2 cut(s) 208, 1701
BseBI CCWGG 4 cut(s) 37, 930, 989, 1332
BseDI CCNNGG 8 cut(s) 36, 129, 928, 987, 1302, 1522, 1605, 1671
BseGI GGATG 5 cut(s) 144, 253, 407, 507, 750
BseLI CCNNNNNNNGG 7 cut(s) 36, 135, 935, 1337, 1523, 1696, 1697
BseMI GCAATG 2 cut(s) 208, 1701
BseMII CTCAG 3 cut(s) 542, 1130, 1638
BseRI GAGGAG 1 cut(s) 1716
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 5 cut(s) 356, 1294, 1330, 1547, 1603
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 3 cut(s) 134, 190, 812
BshNI GGYRCC 2 cut(s) 227, 356
BsiHKAI GWGCWC 2 cut(s) 162, 1163
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 3 cut(s) 130, 135, 1467
BslI CCNNNNNNNGG 7 cut(s) 36, 135, 935, 1337, 1523, 1696, 1697
BsmI GAATGC 2 cut(s) 433, 1432
BsnI GGCC 3 cut(s) 134, 190, 812
BsoBI CYCGRG 1 cut(s) 129
Bsp1286I GDGCHC 5 cut(s) 162, 232, 499, 988, 1163
Bsp143I GATC 5 cut(s) 504, 756, 1142, 1396, 1579
BspACI CCGC 5 cut(s) 98, 382, 416, 845, 1602
BspANI GGCC 3 cut(s) 134, 190, 812
BspCNI CTCAG 3 cut(s) 541, 1131, 1639
BspFNI CGCG 1 cut(s) 384
BspLI GGNNCC 4 cut(s) 189, 229, 358, 985
BspMI ACCTGC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 1248
BspPI GGATC 2 cut(s) 751, 1574
BspQI GCTCTTC 2 cut(s) 150, 996
BspT107I GGYRCC 2 cut(s) 227, 356
BsrDI GCAATG 2 cut(s) 208, 1701
BssECI CCNNGG 8 cut(s) 36, 129, 928, 987, 1302, 1522, 1605, 1671
BssMI GATC 5 cut(s) 504, 756, 1142, 1396, 1579
BssT1I CCWWGG 4 cut(s) 1302, 1522, 1605, 1671
Bst2UI CCWGG 4 cut(s) 37, 930, 989, 1332
Bst4CI ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
Bst6I CTCTTC 3 cut(s) 150, 482, 996
BstC8I GCNNGC 2 cut(s) 162, 1246
BstDEI CTNAG 6 cut(s) 143, 440, 528, 582, 1139, 1647
BstF5I GGATG 5 cut(s) 144, 253, 407, 507, 750
BstFNI CGCG 1 cut(s) 384
BstH2I RGCGCY 1 cut(s) 1198
BstHHI GCGC 2 cut(s) 386, 1197
BstKTI GATC 5 cut(s) 507, 759, 1145, 1399, 1582
BstMBI GATC 5 cut(s) 504, 756, 1142, 1396, 1579
BstMWI GCNNNNNNNGC 8 cut(s) 32, 41, 923, 1250, 1295, 1304, 1463, 1713
BstNI CCWGG 4 cut(s) 37, 930, 989, 1332
BstNSI RCATGY 1 cut(s) 1053
BstSCI CCNGG 7 cut(s) 35, 128, 129, 134, 928, 987, 1330
BstSFI CTRYAG 4 cut(s) 258, 369, 789, 1311
BstSLI GKGCMC 1 cut(s) 232
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 5 cut(s) 356, 1294, 1330, 1547, 1603
BstV2I GAAGAC 1 cut(s) 747
BsuI GTATCC 1 cut(s) 1456
BsuRI GGCC 3 cut(s) 134, 190, 812
BtsCI GGATG 5 cut(s) 144, 253, 407, 507, 750
BtsIMutI CAGTG 3 cut(s) 255, 417, 638
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 162, 1246
CfoI GCGC 2 cut(s) 386, 1197
Cfr13I GGNCC 4 cut(s) 132, 188, 436, 964
Cfr9I CCCGGG 1 cut(s) 129
CseI GACGC 1 cut(s) 207
CsiI ACCWGGT 1 cut(s) 1330
Csp6I GTAC 3 cut(s) 357, 537, 1328
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 5 cut(s) 761, 1050, 1558, 1708, 1717
CviQI GTAC 3 cut(s) 357, 537, 1328
DdeI CTNAG 6 cut(s) 143, 440, 528, 582, 1139, 1647
DpnI GATC 5 cut(s) 506, 758, 1144, 1398, 1581
DpnII GATC 5 cut(s) 504, 756, 1142, 1396, 1579
Eam1104I CTCTTC 3 cut(s) 150, 482, 996
EarI CTCTTC 3 cut(s) 150, 482, 996
Ecl136II GAGCTC 1 cut(s) 160
Eco130I CCWWGG 4 cut(s) 1302, 1522, 1605, 1671
Eco24I GRGCYC 3 cut(s) 162, 499, 988
Eco47I GGWCC 2 cut(s) 436, 964
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 3 cut(s) 417, 537, 1020
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 964
EcoRII CCWGG 4 cut(s) 35, 928, 987, 1330
EcoT14I CCWWGG 4 cut(s) 1302, 1522, 1605, 1671
EcoT38I GRGCYC 3 cut(s) 162, 499, 988
ErhI CCWWGG 4 cut(s) 1302, 1522, 1605, 1671
FaeI CATG 5 cut(s) 764, 1053, 1561, 1711, 1720
FalI AAGNNNNNCTT 2 cut(s) 1182, 1214
FatI CATG 5 cut(s) 760, 1049, 1557, 1707, 1716
FauI CCCGC 1 cut(s) 389
FbaI TGATCA 1 cut(s) 504
FblI GTMKAC 1 cut(s) 1554
Fnu4HI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
FokI GGATG 5 cut(s) 151, 260, 414, 514, 737
FriOI GRGCYC 3 cut(s) 162, 499, 988
Fsp4HI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
FspBI CTAG 7 cut(s) 45, 212, 306, 869, 968, 1245, 1726
GlaI GCGC 2 cut(s) 385, 1196
GluI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
HaeII RGCGCY 1 cut(s) 1198
HaeIII GGCC 3 cut(s) 134, 190, 812
HapII CCGG 3 cut(s) 130, 135, 1467
HgaI GACGC 1 cut(s) 207
HhaI GCGC 2 cut(s) 386, 1197
Hin1II CATG 5 cut(s) 764, 1053, 1561, 1711, 1720
Hin6I GCGC 2 cut(s) 384, 1195
HinP1I GCGC 2 cut(s) 384, 1195
HindIII AAGCTT 2 cut(s) 220, 890
HinfI GANTC 4 cut(s) 114, 467, 1470, 1572
HpaII CCGG 3 cut(s) 130, 135, 1467
HphI GGTGA 5 cut(s) 581, 700, 1115, 1274, 1556
Hpy166II GTNNAC 2 cut(s) 1133, 1555
Hpy188I TCNGA 7 cut(s) 113, 501, 517, 717, 1014, 1168, 1425
Hpy188III TCNNGA 7 cut(s) 84, 212, 292, 754, 1228, 1435, 1517
Hpy8I GTNNAC 2 cut(s) 1133, 1555
HpyAV CCTTC 8 cut(s) 81, 392, 659, 943, 1264, 1313, 1358, 1675
HpyCH4III ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 41, 923, 1250, 1295, 1304, 1463, 1713
HpyF3I CTNAG 6 cut(s) 143, 440, 528, 582, 1139, 1647
Hsp92II CATG 5 cut(s) 764, 1053, 1561, 1711, 1720
HspAI GCGC 2 cut(s) 384, 1195
KpnI GGTACC 1 cut(s) 360
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 5 cut(s) 504, 756, 1142, 1396, 1579
LguI GCTCTTC 2 cut(s) 150, 996
LmnI GCTCC 3 cut(s) 983, 1241, 1504
Lsp1109I GCAGC 5 cut(s) 356, 1294, 1330, 1547, 1603
LweI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
MabI ACCWGGT 1 cut(s) 1330
MaeI CTAG 7 cut(s) 45, 212, 306, 869, 968, 1245, 1726
MaeIII GTNAC 7 cut(s) 178, 238, 253, 686, 1103, 1185, 1279
MalI GATC 5 cut(s) 506, 758, 1144, 1398, 1581
MboI GATC 5 cut(s) 504, 756, 1142, 1396, 1579
MfeI CAATTG 1 cut(s) 1149
MhlI GDGCHC 5 cut(s) 162, 232, 499, 988, 1163
MlyI GAGTC 1 cut(s) 1479
MmeI TCCRAC 1 cut(s) 633
MseI TTAA 4 cut(s) 174, 906, 921, 1563
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 1138
MspI CCGG 3 cut(s) 130, 135, 1467
MspR9I CCNGG 7 cut(s) 37, 130, 131, 136, 930, 989, 1332
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 2 cut(s) 433, 1432
MvaI CCWGG 4 cut(s) 37, 930, 989, 1332
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 8 cut(s) 32, 41, 923, 1250, 1295, 1304, 1463, 1713
NciI CCSGG 3 cut(s) 130, 131, 136
NdeII GATC 5 cut(s) 504, 756, 1142, 1396, 1579
NheI GCTAGC 1 cut(s) 1244
NlaIII CATG 5 cut(s) 764, 1053, 1561, 1711, 1720
NlaIV GGNNCC 4 cut(s) 189, 229, 358, 985
NmuCI GTSAC 4 cut(s) 178, 238, 253, 1103
NspI RCATGY 1 cut(s) 1053
PciSI GCTCTTC 2 cut(s) 150, 996
PctI GAATGC 2 cut(s) 433, 1432
PfeI GAWTC 3 cut(s) 114, 467, 1572
PflFI GACNNNGTC 1 cut(s) 1082
PkrI GCNGC 7 cut(s) 100, 346, 418, 1309, 1345, 1537, 1618
PleI GAGTC 1 cut(s) 1478
PpsI GAGTC 1 cut(s) 1478
PpuMI RGGWCCY 1 cut(s) 964
PsiI TTATAA 1 cut(s) 884
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 964
Psp6I CCWGG 4 cut(s) 35, 928, 987, 1330
PspGI CCWGG 4 cut(s) 35, 928, 987, 1330
PspN4I GGNNCC 4 cut(s) 189, 229, 358, 985
PspPI GGNCC 4 cut(s) 132, 188, 436, 964
PspPPI RGGWCCY 1 cut(s) 964
PsyI GACNNNGTC 1 cut(s) 1082
PvuII CAGCTG 1 cut(s) 1138
RsaI GTAC 3 cut(s) 358, 538, 1329
RsaNI GTAC 3 cut(s) 357, 537, 1328
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 162
SapI GCTCTTC 2 cut(s) 150, 996
SaqAI TTAA 4 cut(s) 174, 906, 921, 1563
SatI GCNGC 7 cut(s) 99, 345, 417, 1308, 1344, 1536, 1617
Sau3AI GATC 5 cut(s) 504, 756, 1142, 1396, 1579
Sau96I GGNCC 4 cut(s) 132, 188, 436, 964
SchI GAGTC 1 cut(s) 1479
ScrFI CCNGG 7 cut(s) 37, 130, 131, 136, 930, 989, 1332
SduI GDGCHC 5 cut(s) 162, 232, 499, 988, 1163
SexAI ACCWGGT 1 cut(s) 1330
SfaNI GCATC 8 cut(s) 129, 157, 331, 392, 1276, 1428, 1522, 1603
SfcI CTRYAG 4 cut(s) 258, 369, 789, 1311
SinI GGWCC 2 cut(s) 436, 964
SmaI CCCGGG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 1433
SmoI CTYRAG 1 cut(s) 1433
SrfI GCCCGGGC 1 cut(s) 131
SsiI CCGC 5 cut(s) 98, 382, 416, 845, 1602
SspI AATATT 1 cut(s) 801
SspMI CTAG 7 cut(s) 45, 212, 306, 869, 968, 1245, 1726
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 7 cut(s) 35, 128, 129, 134, 928, 987, 1330
StyI CCWWGG 4 cut(s) 1302, 1522, 1605, 1671
TaaI ACNGT 6 cut(s) 53, 259, 373, 642, 1066, 1084
TaqI TCGA 5 cut(s) 83, 1020, 1395, 1473, 1662
TauI GCSGC 2 cut(s) 101, 419
TfiI GAWTC 3 cut(s) 114, 467, 1572
Tru1I TTAA 4 cut(s) 174, 906, 921, 1563
Tru9I TTAA 4 cut(s) 174, 906, 921, 1563
TscAI CASTG 3 cut(s) 262, 417, 645
TseFI GTSAC 4 cut(s) 178, 238, 253, 1103
TseI GCWGC 5 cut(s) 344, 1307, 1343, 1535, 1616
Tsp45I GTSAC 4 cut(s) 178, 238, 253, 1103
TspDTI ATGAA 2 cut(s) 440, 686
TspGWI ACGGA 2 cut(s) 460, 567
TspMI CCCGGG 1 cut(s) 129
TspRI CASTG 3 cut(s) 262, 417, 645
Tth111I GACNNNGTC 1 cut(s) 1082
VpaK11BI GGWCC 2 cut(s) 436, 964
XapI RAATTY 2 cut(s) 784, 955
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 1053
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 129
XmiI GTMKAC 1 cut(s) 1554
XspI CTAG 7 cut(s) 45, 212, 306, 869, 968, 1245, 1726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.