Rh4BG068300
TCP Family

Belongs to the chaperonin (HSP60) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004623
Physical Location & Seq
Forward (+)
12209319 .. 12214297
4979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG068300.1

Sequence Viewer

Length: 1596 bp
ATGCTCAATGGTGTCGAAGACCTGGCCGATGCTGTTAAAGTCACAATGGGCCCTAAGGGTCGTAATGTGGTTATTGAGCAAAGCTTTGGCGCACCCAAAGTAACAAAAGATGGTGTAACAGTTGCAAAAAGTATCGAGTTTAAGGATAAAGTTAAGAATGTTGGAGCTAGCCTTGTAAAACAGGTTGCTAATGCCACAAATGATGTAGCGGGTGATGGTACTACTTGTGCTACAATCCTGACAAGAGCTATCTACACTGAAGGATGCAAGTCAGTTGCTGCTGGTATGAACGCCATGGACCTAAGACGTGGTATTAACATGGCAGTTGATTCTGTAGTAACTAACTTGAAGAGCAGAGCCCGAATGATTAGCACTTCTGAAGAGATAGCACAGGTCGGAACAATATCGGCAAATGGCGAGAGAGAAATCGGTGAATTGATTGCAAAGGCCATGGAGAAGGTTGGCAAGGAAGGAGTAATCACGATCGCTGATGGAAAAACATTGTATAACGAGTTGGAAGTTGTTGAGGGCATGAAGCTAGACAGAGGCTACATATCTCCTCATTTCATCACCAACACAAAAAATCAAAAATGTGAATTGGAAGATCCTTTAGTTCTAATCCATGAGAAGAAAGTCTCAAATATTAATGCTGTGATTAAAGTATTGGAGTTGGCTTTGAAGAGGCAAAAGCCTTTGCTGATTGTCGCAGAAGATGTGGAAAGTGATGCACTTGCAACACTTATATTAAATAAGCTTCGTGCTGGAATCAAGGTTTGTGCTATCAAAGCCCCTGGTTTCGGGGAAAATAGGAAATCCAATCTGCAGGATCTTGCAGTTCTCACAGGTAGCACTGTTATTACTGAAGAGCTGGGTTTGAACCTTGAGAAAGTGGATCTGGATATGCTTGGAACTTGCAAAAAGGTCACAATTTCAAAAGATGACACTGTAATTCTTGACGGTGCTGGTGACAAGAAGGAAATTGAGGAAAGATGTGAACAGATTCGGTCAGCAGTTGAAGCAAGCACTTCTGATTATGACAGAGAGAAGTTACAGGAGAGACTGGCAAAACTTTCTGGTGGTGTTGCTGTGTTGAAGATTGGAGGAGCTAGTGAAGCTGAAGTTAGTGAGAAGAAGGATAGGGTTACTGATGCCCTGAATGCCACAAAGGCAGCTGTTGAAGAGGGTATAGTAGCAGGTGGTGGTGTTGCAGTCCTTTATGCAGCTAAAGAGTTGGACAAACTTCCAACTGCTAACTTTGATCAGAAGATTGGAGTTCAGATTATTCAGAATGCTCTGAAGGCACCTGTCCACACTATTGCTTCTAATGCGGGGGTTGAAGGAGCTGTTGTTGTTGGTAAGCTCTTGGAGCAAGATAACCCTGATCTGGGATATGATGCAGCTAAAGGTGAATATGTTGATATGGTCAAAGCTGGAATAATAGATCCTTTGAAAGTAATTAGGACCGCTTTAACGGATGCTGCAAGTATTTCATCTTTGCTGACAACTACTGAAGCCGTCGTAGTTGAACTTCCTAGTGACAAGGAGACCCCGGCAATGGGCGGTGGCATGGGTGGCATGGGTGGCATGGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005507 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006457 GO:0006458 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017038 GO:0017076 GO:0022626 GO:0030554 GO:0031090 GO:0031967 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0035639 GO:0036094 GO:0042221 GO:0042788 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044183 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044743 GO:0045041 GO:0045184 GO:0046686 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051082 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061077 GO:0065002 GO:0070013 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0071944 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098805 GO:1901265 GO:1901363 GO:1990542 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03018 ko04212 ko04940 ko05134 ko05152 map03018 map04212 map04940 map05134 map05152
Pfam Domains
Protein Families
Cpn60_TCP1

Protein Analysis

531

Amino Acids

56.1

Weight (kDa)

5.16

Isoelectric Point (pI)

22.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 8 - 508 1.5e-68 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1184
Acc36I ACCTGC 1 cut(s) 1184
AccB1I GGYRCC 1 cut(s) 1300
AciI CCGC 4 cut(s) 209, 1328, 1464, 1560
AclWI GGATC 4 cut(s) 599, 834, 900, 1436
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 6 cut(s) 279, 399, 882, 1137, 1316, 1530
AfaI GTAC 1 cut(s) 220
AfiI CCNNNNNNNGG 5 cut(s) 797, 1384, 1385, 1555, 1556
AjiI CACGTC 1 cut(s) 308
AjnI CCWGG 2 cut(s) 21, 790
Alw26I GTCTC 3 cut(s) 640, 1051, 1538
AlwI GGATC 4 cut(s) 599, 834, 900, 1436
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 3 cut(s) 24, 49, 447
ApaI GGGCCC 1 cut(s) 53
ApeKI GCWGC 5 cut(s) 278, 1169, 1220, 1397, 1478
AseI ATTAAT 1 cut(s) 645
Asp700I GAANNNNTTC 1 cut(s) 999
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 4 cut(s) 49, 50, 298, 1461
AsuC2I CCSGG 1 cut(s) 1550
AsuHPI GGTGA 5 cut(s) 224, 443, 562, 977, 1418
AsuNHI GCTAGC 1 cut(s) 167
AvaII GGWCC 2 cut(s) 298, 1461
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 53
BanI GGYRCC 1 cut(s) 1300
BanII GRGCYC 2 cut(s) 53, 361
BarI GAAGNNNNNNTAC 2 cut(s) 1512, 1544
BbsI GAAGAC 1 cut(s) 24
BbvI GCAGC 5 cut(s) 265, 1181, 1232, 1409, 1465
BccI CCATC 3 cut(s) 104, 209, 485
BceAI ACGGC 1 cut(s) 1499
BciT130I CCWGG 2 cut(s) 23, 792
BclI TGATCA 1 cut(s) 1258
BcnI CCSGG 1 cut(s) 1550
BcoDI GTCTC 3 cut(s) 640, 1051, 1538
BfaI CTAG 4 cut(s) 168, 539, 1107, 1533
BfmI CTRYAG 2 cut(s) 333, 821
BfuAI ACCTGC 1 cut(s) 1184
BglI GCCNNNNNGGC 1 cut(s) 1166
BisI GCNGC 5 cut(s) 279, 1170, 1221, 1398, 1479
BlsI GCNGC 5 cut(s) 280, 1171, 1222, 1399, 1480
Bme1390I CCNGG 3 cut(s) 23, 792, 1550
Bme18I GGWCC 2 cut(s) 298, 1461
BmgBI CACGTC 1 cut(s) 308
BmgT120I GGNCC 4 cut(s) 49, 50, 298, 1461
BmiI GGNNCC 2 cut(s) 51, 1302
BmrFI CCNGG 3 cut(s) 23, 792, 1550
BmsI GCATC 6 cut(s) 19, 254, 715, 1138, 1384, 1465
BmtI GCTAGC 1 cut(s) 171
BpiI GAAGAC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 902
BpuMI CCSGG 1 cut(s) 1550
BsaI GGTCTC 1 cut(s) 1538
BsaJI CCNNGG 4 cut(s) 294, 450, 790, 1548
BsaXI ACNNNNNCTCC 2 cut(s) 1332, 1362
Bsc4I CCNNNNNNNGG 5 cut(s) 797, 1384, 1385, 1555, 1556
Bse1I ACTGG 1 cut(s) 1065
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 1 cut(s) 1560
BseBI CCWGG 2 cut(s) 23, 792
BseDI CCNNGG 4 cut(s) 294, 450, 790, 1548
BseGI GGATG 2 cut(s) 269, 1480
BseLI CCNNNNNNNGG 5 cut(s) 797, 1384, 1385, 1555, 1556
BseMI GCAATG 1 cut(s) 1560
BseNI ACTGG 1 cut(s) 1065
BseRI GAGGAG 2 cut(s) 549, 1116
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 5 cut(s) 265, 1181, 1232, 1409, 1465
BseYI CCCAGC 1 cut(s) 868
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 3 cut(s) 26, 51, 449
BshNI GGYRCC 1 cut(s) 1300
BsiEI CGRYCG 1 cut(s) 486
BsiSI CCGG 1 cut(s) 1550
BslI CCNNNNNNNGG 5 cut(s) 797, 1384, 1385, 1555, 1556
BsmAI GTCTC 3 cut(s) 640, 1051, 1538
BsmI GAATGC 2 cut(s) 1162, 1294
BsnI GGCC 3 cut(s) 26, 51, 449
Bso31I GGTCTC 1 cut(s) 1538
Bsp120I GGGCCC 1 cut(s) 49
Bsp1286I GDGCHC 2 cut(s) 53, 361
Bsp143I GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
Bsp19I CCATGG 2 cut(s) 294, 450
BspACI CCGC 4 cut(s) 209, 1328, 1464, 1560
BspANI GGCC 3 cut(s) 26, 51, 449
BspLI GGNNCC 2 cut(s) 51, 1302
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 1184
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 4 cut(s) 599, 834, 900, 1436
BspQI GCTCTTC 2 cut(s) 344, 858
BspT107I GGYRCC 1 cut(s) 1300
BspTNI GGTCTC 1 cut(s) 1538
BsrDI GCAATG 1 cut(s) 1560
BsrI ACTGG 1 cut(s) 1065
BssECI CCNNGG 4 cut(s) 294, 450, 790, 1548
BssMI GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
BssT1I CCWWGG 2 cut(s) 294, 450
Bst2UI CCWGG 2 cut(s) 23, 792
Bst4CI ACNGT 4 cut(s) 121, 853, 946, 959
Bst6I CTCTTC 5 cut(s) 344, 375, 674, 858, 1173
BstC8I GCNNGC 2 cut(s) 169, 1021
BstDEI CTNAG 2 cut(s) 54, 302
BstDSI CCRYGG 2 cut(s) 294, 450
BstF5I GGATG 2 cut(s) 269, 1480
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 7 cut(s) 486, 607, 829, 895, 1261, 1384, 1444
BstMAI GTCTC 3 cut(s) 640, 1051, 1538
BstMBI GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
BstMCI CGRYCG 1 cut(s) 486
BstNI CCWGG 2 cut(s) 23, 792
BstSCI CCNGG 3 cut(s) 21, 790, 1548
BstSFI CTRYAG 2 cut(s) 333, 821
BstSLI GKGCMC 1 cut(s) 53
BstV1I GCAGC 5 cut(s) 265, 1181, 1232, 1409, 1465
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 4 cut(s) 604, 826, 892, 1441
BstYI RGATCY 4 cut(s) 604, 826, 892, 1441
Bsu36I CCTNAGG 1 cut(s) 54
BsuRI GGCC 3 cut(s) 26, 51, 449
BtgI CCRYGG 2 cut(s) 294, 450
BtrI CACGTC 1 cut(s) 308
BtsCI GGATG 2 cut(s) 269, 1480
BtsIMutI CAGTG 3 cut(s) 255, 849, 942
BveI ACCTGC 1 cut(s) 1184
Cac8I GCNNGC 2 cut(s) 169, 1021
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 49, 50, 298, 1461
Csp6I GTAC 1 cut(s) 219
CviAII CATG 8 cut(s) 295, 319, 451, 532, 623, 1567, 1576, 1585
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 2 cut(s) 54, 302
DpnI GATC 7 cut(s) 485, 606, 828, 894, 1260, 1383, 1443
DpnII GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 5 cut(s) 344, 375, 674, 858, 1173
EarI CTCTTC 5 cut(s) 344, 375, 674, 858, 1173
Eco130I CCWWGG 2 cut(s) 294, 450
Eco24I GRGCYC 2 cut(s) 53, 361
Eco31I GGTCTC 1 cut(s) 1538
Eco47I GGWCC 2 cut(s) 298, 1461
Eco57I CTGAAG 6 cut(s) 279, 399, 882, 1137, 1316, 1530
Eco81I CCTNAGG 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 21, 790
EcoT14I CCWWGG 2 cut(s) 294, 450
EcoT38I GRGCYC 2 cut(s) 53, 361
ErhI CCWWGG 2 cut(s) 294, 450
FaeI CATG 8 cut(s) 298, 322, 454, 535, 626, 1570, 1579, 1588
FatI CATG 8 cut(s) 294, 318, 450, 531, 622, 1566, 1575, 1584
FauI CCCGC 2 cut(s) 202, 1321
FbaI TGATCA 1 cut(s) 1258
Fnu4HI GCNGC 5 cut(s) 279, 1170, 1221, 1398, 1479
FokI GGATG 2 cut(s) 276, 1487
FriOI GRGCYC 2 cut(s) 53, 361
Fsp4HI GCNGC 5 cut(s) 279, 1170, 1221, 1398, 1479
FspBI CTAG 4 cut(s) 168, 539, 1107, 1533
GlaI GCGC 1 cut(s) 91
GluI GCNGC 5 cut(s) 279, 1170, 1221, 1398, 1479
GsaI CCCAGC 1 cut(s) 872
HaeIII GGCC 3 cut(s) 26, 51, 449
HapII CCGG 1 cut(s) 1550
HhaI GCGC 1 cut(s) 92
Hin1II CATG 8 cut(s) 298, 322, 454, 535, 626, 1570, 1579, 1588
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HindIII AAGCTT 2 cut(s) 82, 752
HinfI GANTC 3 cut(s) 329, 765, 1000
HpaII CCGG 1 cut(s) 1550
HphI GGTGA 5 cut(s) 224, 443, 562, 977, 1418
Hpy166II GTNNAC 2 cut(s) 995, 1309
Hpy188I TCNGA 7 cut(s) 379, 398, 1030, 1263, 1278, 1287, 1296
Hpy188III TCNNGA 4 cut(s) 238, 481, 896, 953
Hpy8I GTNNAC 2 cut(s) 995, 1309
Hpy99I CGWCG 1 cut(s) 1520
HpyAV CCTTC 7 cut(s) 254, 451, 464, 967, 1126, 1291, 1331
HpyCH4III ACNGT 4 cut(s) 121, 853, 946, 959
HpyCH4IV ACGT 1 cut(s) 307
HpyF3I CTNAG 2 cut(s) 54, 302
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 8 cut(s) 298, 322, 454, 535, 626, 1570, 1579, 1588
HspAI GCGC 1 cut(s) 90
Ksp22I TGATCA 1 cut(s) 1258
Kzo9I GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
LguI GCTCTTC 2 cut(s) 344, 858
LmnI GCTCC 4 cut(s) 164, 1103, 1340, 1366
Lsp1109I GCAGC 5 cut(s) 265, 1181, 1232, 1409, 1465
LweI GCATC 6 cut(s) 19, 254, 715, 1138, 1384, 1465
MaeI CTAG 4 cut(s) 168, 539, 1107, 1533
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 9 cut(s) 40, 100, 115, 337, 922, 965, 1047, 1141, 1535
MalI GATC 7 cut(s) 485, 606, 828, 894, 1260, 1383, 1443
MboI GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
MflI RGATCY 4 cut(s) 604, 826, 892, 1441
MhlI GDGCHC 2 cut(s) 53, 361
MluCI AATT 6 cut(s) 434, 596, 927, 948, 978, 1455
MmeI TCCRAC 5 cut(s) 142, 376, 495, 1212, 1268
MnlI CCTC 7 cut(s) 520, 539, 570, 675, 976, 1094, 1174
MroXI GAANNNNTTC 1 cut(s) 999
MseI TTAA 8 cut(s) 36, 141, 153, 315, 645, 657, 746, 1469
MspA1I CMGCKG 1 cut(s) 1172
MspI CCGG 1 cut(s) 1550
MspR9I CCNGG 3 cut(s) 23, 792, 1550
Mva1269I GAATGC 2 cut(s) 1162, 1294
MvaI CCWGG 2 cut(s) 23, 792
NciI CCSGG 1 cut(s) 1550
NcoI CCATGG 2 cut(s) 294, 450
NdeII GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
NheI GCTAGC 1 cut(s) 167
NlaIII CATG 8 cut(s) 298, 322, 454, 535, 626, 1570, 1579, 1588
NlaIV GGNNCC 2 cut(s) 51, 1302
NmuCI GTSAC 4 cut(s) 40, 922, 965, 1535
PaqCI CACCTGC 1 cut(s) 1184
PciSI GCTCTTC 2 cut(s) 344, 858
PctI GAATGC 2 cut(s) 1162, 1294
PdmI GAANNNNTTC 1 cut(s) 999
PfeI GAWTC 3 cut(s) 329, 765, 1000
PkrI GCNGC 5 cut(s) 280, 1171, 1222, 1399, 1480
Ple19I CGATCG 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 645
Psp6I CCWGG 2 cut(s) 21, 790
PspFI CCCAGC 1 cut(s) 868
PspGI CCWGG 2 cut(s) 21, 790
PspN4I GGNNCC 2 cut(s) 51, 1302
PspOMI GGGCCC 1 cut(s) 49
PspPI GGNCC 4 cut(s) 49, 50, 298, 1461
PstI CTGCAG 1 cut(s) 825
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 4 cut(s) 604, 826, 892, 1441
PvuI CGATCG 1 cut(s) 486
PvuII CAGCTG 1 cut(s) 1172
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
SapI GCTCTTC 2 cut(s) 344, 858
SaqAI TTAA 8 cut(s) 36, 141, 153, 315, 645, 657, 746, 1469
SatI GCNGC 5 cut(s) 279, 1170, 1221, 1398, 1479
Sau3AI GATC 7 cut(s) 483, 604, 826, 892, 1258, 1381, 1441
Sau96I GGNCC 4 cut(s) 49, 50, 298, 1461
ScrFI CCNGG 3 cut(s) 23, 792, 1550
SduI GDGCHC 2 cut(s) 53, 361
SfaNI GCATC 6 cut(s) 19, 254, 715, 1138, 1384, 1465
SfcI CTRYAG 2 cut(s) 333, 821
SinI GGWCC 2 cut(s) 298, 1461
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
Sse9I AATT 6 cut(s) 434, 596, 927, 948, 978, 1455
SsiI CCGC 4 cut(s) 209, 1328, 1464, 1560
SspI AATATT 1 cut(s) 643
SspMI CTAG 4 cut(s) 168, 539, 1107, 1533
StyD4I CCNGG 3 cut(s) 21, 790, 1548
StyI CCWWGG 2 cut(s) 294, 450
TaaI ACNGT 4 cut(s) 121, 853, 946, 959
TaiI ACGT 1 cut(s) 310
TaqI TCGA 2 cut(s) 15, 135
TaqII GACCGA 1 cut(s) 993
TasI AATT 6 cut(s) 434, 596, 927, 948, 978, 1455
TfiI GAWTC 3 cut(s) 329, 765, 1000
Tru1I TTAA 8 cut(s) 36, 141, 153, 315, 645, 657, 746, 1469
Tru9I TTAA 8 cut(s) 36, 141, 153, 315, 645, 657, 746, 1469
TscAI CASTG 3 cut(s) 262, 856, 949
TseFI GTSAC 4 cut(s) 40, 922, 965, 1535
TseI GCWGC 5 cut(s) 278, 1169, 1220, 1397, 1478
Tsp45I GTSAC 4 cut(s) 40, 922, 965, 1535
TspDTI ATGAA 4 cut(s) 302, 548, 556, 1479
TspGWI ACGGA 1 cut(s) 1487
TspRI CASTG 3 cut(s) 262, 856, 949
VpaK11BI GGWCC 2 cut(s) 298, 1461
VspI ATTAAT 1 cut(s) 645
XmnI GAANNNNTTC 1 cut(s) 999
XspI CTAG 4 cut(s) 168, 539, 1107, 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.