MD05G1196000.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005506
Physical Location & Seq
Reverse (-)
32335969 .. 32341012
5044 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1196000.v1.1.491

Sequence Viewer

Length: 2394 bp
ATGCCTCAACTCACCAGTTCTAACCGTTTGATTCAGAGCTGTGTTTGTGAGATGGACATAGCATCTATAAAATGTCTGATCAACAGTATATCTCGATTTGTTCACCTAGTTTCAAGCCAAACATCGAAACATATTCCTATTCAGAAGGATTACAGAACCATAGTTGATGTACTAAAGCTTTTGAAGTCATTGCTTGATGAAGTAGTTGACTTTAAAATACCGTCAGATGACATTCTATATAAAGAGTGTGATGAATTGGATATGGCTGTAAATGGAGCTAGAGAATTCATGGAAAAATGGTCTCCAAAGTCGAGCAAAATTTTAAGTGCTTGGTGGGGTGAACCATTGTTGATAACAATCCAAAGCTCATCACTAAAGATTTGCGGCATATTACCTAGACTGTTGCAGTCATCTTCATCTGGCTCAATCTTAGCAGGTCTTCAGCACTGTATGCAGGAAATTCAATCTTTGAAGCTGGAAAGAGTATCAGAATACTTAGAAGAGGCTCTGAAAAGTCCAAGAAAAGACGTCATGCCTTCCACCAAACTTCTTATGAAAATTATCGAATTGCTTAATCTGACTTCAAACCAAGAACTGCTGAAAGAAAGCATTGCTGTAGAAAAGGAAAGGATGAATGTCGAAGTCAGCGATGTTAGAGGGGAATTAGATCAAATTAACCAAATTGTGGTTCTCGTCTCCCATATTCGTGAATTCATGGTCAAAATTGAGCACCTTGAAACTGCATCTGGCGTCCCAATCCCTTCGTACTTCCGCTGCCCTTTGTCTTCAGAACTAATGCTGGACCCTGTGATCGTAGCTTCAGGTCAAACCTATGAGAGATCTTCCATCCAAAAATGGATTGATCATGGGCTAACTCTCTGCCCAAAGACCCGACAGAGGCTTTCCCATACAAATCTCCTTCCCAATTATACTGTCAAAGCTATGATAGAAAGCTGGTGTCAGGAAAATGATATAAAACTTACGGCGAACTCTGACGGCTCTAATGCTATCTTGGTCCCATCTCCATCAGATGCAGATTCAATTCACACGGATAGTTTGTATTGCTCTACACACAGTAGCAATTCGACATCGAGGTCCTCTCTTGAAGTTGGAAACGTATTTGAGAAACAAAAGAAAGATGTCTCTCCTAGGTTAGGTGGACAAAAGTCCAATGAATGCGATAGCAACAAGAAAGGCCACTCATCACCAAAACAGTCATATAGTCATTGCAGGAGTGAATCAGCCACTAGTGCTGCTTCCAGTATTGATTATGCACTGCCAGCATCCATTAAGGTGTCGGGGATATCTAATAAGCATGAAAATGTTCGTAGGTTGTCTGGAGAAATCTCATCTGCGCATGAAGTTGCTTCTTCTCCAGATAAAGAAACAACAATACCCCCCGGGTTATCAGGAAAATTATCTCACATTTCCAAGGCAAAAGCAGAAGGGGCATGGACTGACAGCCCCAGTTATCCCAACAGACAGCTGCTTCCATTTTCTAGTTCAGGATCTGACGAGTTGACGACAGCATCCCGTGTCAGGAAATTAATTGAAGACCTCCAGAGCCAGTCCAATGAAGTGCAAACTATGGCGGCAGAAGAGTTGCGGCTTCTTGGGAAGCGCAATACGGAGAATCGAACTATTATAGGTCAGTGTGGTGCTATCTCGTCGTTACTTTCACTTCTATATTCAGGAGTGATGATAACACAAGAGCATGCTGTGACAGCTCTTTTGAATTTATCAATTAATGAAGACAACAAAGCAATGATTGCAGAAGCAGGGGCAATTGATCCCCTAATCCATGTTCTAAAAACAGGAAGTGACGGAGCCAAAGAAAATTCTGCTGCAGCTTTGTTCAGCCTCTCTGTATTGGATGAATACAAGGCAAAAATTGGTCGGTCTGGTGCAGTCAAGGCCTTGGTTGATCTTCTAGCCTCAGGGACTCTTAGAGGGAAGAAAGATGCTGCTACTGCATTGTTCAATCTGTCAATTTTTCACGAAAATAAAGCTCGCCTAGTTCAAGCTGGGGCTGTGAAGCATCTTATTGATTTGATGGACCCTGATACTGGGATGGTTGACAAGGCCGTTGCTCTCCTAGCAAATTTGTCCACCATTGCGGAGGGGCGCGTAGCAATTGCACGGGAAGGGGGCATTCCATTACTAGTTGAGATTGTTGAATCAGGTTCCCAGAGAGGAAAGGAAAATGCCGCCTCCATACTGTTGCAATTGTGCCTTCATAGTCCAAAGTTTTGTACTCTTGTTCTTCAGGAAGGAGCTGTCCCTCCCCTGGTTGCCTTATCTCAGTCTGGCACACCAAGAGCAAAGGAAAAGGCACAGCAGCTTCTCAGTCACTTCAGGAATCAGCGAGAAGGGGCAATGGGAAAGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

798

Amino Acids

86.79

Weight (kDa)

6.86

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 19 - 157 1.4e-49 PUB2-like, N-terminal domain
U-box PF04564 253 - 324 7.3e-24 U-box domain
ARM_ARMC5 PF24768 504 - 685 6.1e-11 ARMC5
ARM_PUB PF25598 513 - 787 9e-27 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 576 - 746 6.9e-14 ARMC5
Arm PF00514 583 - 622 4.9e-06 Armadillo/beta-catenin-like repeat
Arm PF00514 626 - 663 8.3e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1093
AatII GACGTC 1 cut(s) 531
Acc16I TGCGCA 1 cut(s) 1356
Acc36I ACCTGC 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 685
AccII CGCG 1 cut(s) 2127
AciI CCGC 6 cut(s) 384, 772, 1592, 1606, 2117, 2208
AclWI GGATC 2 cut(s) 1516, 1784
AcsI RAATTY 7 cut(s) 284, 318, 459, 710, 1735, 1837, 2101
AcuI CTGAAG 5 cut(s) 425, 771, 804, 2249, 2338
AcyI GRCGYC 2 cut(s) 528, 750
AfaI GTAC 3 cut(s) 171, 767, 2254
AfiI CCNNNNNNNGG 6 cut(s) 685, 897, 1154, 1539, 2066, 2287
AhlI ACTAGT 2 cut(s) 1247, 2161
AjnI CCWGG 1 cut(s) 2286
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 3 cut(s) 306, 700, 1147
AlwI GGATC 2 cut(s) 1516, 1784
AlwNI CAGNNNCTG 1 cut(s) 1511
Ama87I CYCGRG 1 cut(s) 1400
AoxI GGCC 3 cut(s) 1195, 1914, 2082
ApeKI GCWGC 7 cut(s) 774, 1253, 1486, 1844, 1847, 1964, 2338
ApoI RAATTY 7 cut(s) 284, 318, 459, 710, 1735, 1837, 2101
ArsI GACNNNNNNTTYG 2 cut(s) 1714, 1746
AseI ATTAAT 2 cut(s) 1547, 1746
Asp700I GAANNNNTTC 1 cut(s) 1323
AspA2I CCTAGG 1 cut(s) 1148
AspLEI GCGC 3 cut(s) 1357, 1623, 2127
AspS9I GGNCC 4 cut(s) 802, 1015, 1095, 2056
AsuC2I CCSGG 2 cut(s) 1401, 1402
AsuHPI GGTGA 4 cut(s) 4, 95, 350, 1197
AvaI CYCGRG 1 cut(s) 1400
AvaII GGWCC 4 cut(s) 802, 1015, 1095, 2056
AvrII CCTAGG 1 cut(s) 1148
AxyI CCTNAGG 1 cut(s) 1936
BbsI GAAGAC 4 cut(s) 431, 777, 1560, 1758
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 7 cut(s) 761, 1240, 1473, 1831, 1859, 1951, 2350
BccI CCATC 6 cut(s) 46, 854, 1027, 1033, 2047, 2065
BceAI ACGGC 3 cut(s) 999, 1012, 2069
BciT130I CCWGG 1 cut(s) 2288
BclI TGATCA 2 cut(s) 78, 862
BcnI CCSGG 2 cut(s) 1401, 1402
BcoDI GTCTC 3 cut(s) 306, 700, 1147
BcuI ACTAGT 2 cut(s) 1247, 2161
BfmI CTRYAG 2 cut(s) 615, 1845
BfuAI ACCTGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 839
BlnI CCTAGG 1 cut(s) 1148
Bme1390I CCNGG 3 cut(s) 1401, 1402, 2288
Bme18I GGWCC 4 cut(s) 802, 1015, 1095, 2056
BmeT110I CYCGRG 1 cut(s) 1400
BmgT120I GGNCC 4 cut(s) 802, 1015, 1095, 2056
BmiI GGNNCC 5 cut(s) 804, 1017, 1828, 2058, 2185
BmrFI CCNGG 3 cut(s) 1401, 1402, 2288
BmrI ACTGGG 2 cut(s) 1461, 2076
BmsI GCATC 7 cut(s) 71, 752, 1021, 1292, 1538, 1951, 2047
BmuI ACTGGG 2 cut(s) 1461, 2076
BoxI GACNNNNGTC 1 cut(s) 1165
BpiI GAAGAC 4 cut(s) 431, 777, 1560, 1758
BplI GAGNNNNNCTC 2 cut(s) 1084, 1116
BpmI CTGGAG 3 cut(s) 1359, 1359, 1544
BpuMI CCSGG 2 cut(s) 1401, 1402
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 2 cut(s) 528, 750
BsaI GGTCTC 1 cut(s) 306
BsaJI CCNNGG 6 cut(s) 1148, 1399, 1400, 1431, 1917, 2286
Bsc4I CCNNNNNNNGG 6 cut(s) 685, 897, 1154, 1539, 2066, 2287
Bse1I ACTGG 5 cut(s) 15, 1260, 1467, 1567, 2071
Bse21I CCTNAGG 1 cut(s) 1936
Bse3DI GCAATG 6 cut(s) 188, 609, 1225, 1770, 2112, 2382
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 1 cut(s) 2288
BseDI CCNNGG 6 cut(s) 1148, 1399, 1400, 1431, 1917, 2286
BseGI GGATG 6 cut(s) 636, 846, 1283, 1529, 1879, 2076
BseJI GATNNNNATC 1 cut(s) 356
BseLI CCNNNNNNNGG 6 cut(s) 685, 897, 1154, 1539, 2066, 2287
BseMI GCAATG 6 cut(s) 188, 609, 1225, 1770, 2112, 2382
BseMII CTCAG 3 cut(s) 1950, 2315, 2359
BseNI ACTGG 5 cut(s) 15, 1260, 1467, 1567, 2071
BseXI GCAGC 7 cut(s) 761, 1240, 1473, 1831, 1859, 1951, 2350
BseYI CCCAGC 1 cut(s) 2024
BsgI GTGCAG 1 cut(s) 1926
Bsh1236I CGCG 1 cut(s) 2127
BshFI GGCC 3 cut(s) 1197, 1916, 2084
BsiHKAI GWGCWC 1 cut(s) 732
BsiHKCI CYCGRG 1 cut(s) 1400
BsiSI CCGG 1 cut(s) 1401
BslFI GGGAC 4 cut(s) 737, 1001, 1954, 2264
BslI CCNNNNNNNGG 6 cut(s) 685, 897, 1154, 1539, 2066, 2287
BsmAI GTCTC 3 cut(s) 306, 700, 1147
BsmBI CGTCTC 1 cut(s) 700
BsmFI GGGAC 4 cut(s) 737, 1001, 1954, 2264
BsmI GAATGC 2 cut(s) 1181, 2151
BsnI GGCC 3 cut(s) 1197, 1916, 2084
Bso31I GGTCTC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 1400
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
BspACI CCGC 6 cut(s) 384, 772, 1592, 1606, 2117, 2208
BspANI GGCC 3 cut(s) 1197, 1916, 2084
BspCNI CTCAG 3 cut(s) 1949, 2314, 2358
BspFNI CGCG 1 cut(s) 2127
BspLI GGNNCC 5 cut(s) 804, 1017, 1828, 2058, 2185
BspMAI CTGCAG 1 cut(s) 1849
BspMI ACCTGC 1 cut(s) 425
BspPI GGATC 2 cut(s) 1516, 1784
BspTNI GGTCTC 1 cut(s) 306
BsrDI GCAATG 6 cut(s) 188, 609, 1225, 1770, 2112, 2382
BsrI ACTGG 5 cut(s) 15, 1260, 1467, 1567, 2071
BssECI CCNNGG 6 cut(s) 1148, 1399, 1400, 1431, 1917, 2286
BssMI GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
BssNI GRCGYC 2 cut(s) 528, 750
BssT1I CCWWGG 3 cut(s) 1148, 1431, 1917
Bst2UI CCWGG 1 cut(s) 2288
Bst4CI ACNGT 9 cut(s) 26, 86, 222, 402, 449, 934, 1076, 1215, 2220
Bst6I CTCTTC 2 cut(s) 495, 1593
BstACI GRCGYC 2 cut(s) 528, 750
BstAPI GCANNNNNTGC 2 cut(s) 451, 1769
BstC8I GCNNGC 3 cut(s) 1281, 1716, 2011
BstDEI CTNAG 6 cut(s) 430, 496, 1936, 1946, 2301, 2345
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 6 cut(s) 636, 846, 1283, 1529, 1879, 2076
BstFNI CGCG 1 cut(s) 2127
BstHHI GCGC 3 cut(s) 1357, 1623, 2127
BstKTI GATC 8 cut(s) 81, 670, 813, 842, 865, 1511, 1792, 1927
BstMAI GTCTC 3 cut(s) 306, 700, 1147
BstMBI GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
BstMWI GCNNNNNNNGC 9 cut(s) 451, 1250, 1280, 1448, 1724, 1769, 1913, 1970, 2096
BstNI CCWGG 1 cut(s) 2288
BstNSI RCATGY 1 cut(s) 1718
BstPAI GACNNNNGTC 1 cut(s) 1165
BstSCI CCNGG 3 cut(s) 1399, 1400, 2286
BstSFI CTRYAG 2 cut(s) 615, 1845
BstUI CGCG 1 cut(s) 2127
BstV1I GCAGC 7 cut(s) 761, 1240, 1473, 1831, 1859, 1951, 2350
BstV2I GAAGAC 4 cut(s) 431, 777, 1560, 1758
BstX2I RGATCY 2 cut(s) 839, 1508
BstYI RGATCY 2 cut(s) 839, 1508
Bsu36I CCTNAGG 1 cut(s) 1936
BsuRI GGCC 3 cut(s) 1197, 1916, 2084
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 6 cut(s) 636, 846, 1283, 1529, 1879, 2076
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 3 cut(s) 445, 1274, 1658
BveI ACCTGC 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 1281, 1716, 2011
CaiI CAGNNNCTG 1 cut(s) 1511
CfoI GCGC 3 cut(s) 1357, 1623, 2127
Cfr13I GGNCC 4 cut(s) 802, 1015, 1095, 2056
Cfr9I CCCGGG 1 cut(s) 1400
CseI GACGC 1 cut(s) 739
Csp6I GTAC 3 cut(s) 170, 766, 2253
CviAII CATG 9 cut(s) 289, 532, 715, 866, 1316, 1358, 1452, 1715, 1802
CviQI GTAC 3 cut(s) 170, 766, 2253
DdeI CTNAG 6 cut(s) 430, 496, 1936, 1946, 2301, 2345
DpnI GATC 8 cut(s) 80, 669, 812, 841, 864, 1510, 1791, 1926
DpnII GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
DraI TTTAAA 1 cut(s) 214
DrdI GACNNNNNNGTC 1 cut(s) 1093
DseDI GACNNNNNNGTC 1 cut(s) 1093
Eam1104I CTCTTC 2 cut(s) 495, 1593
EarI CTCTTC 2 cut(s) 495, 1593
Eco130I CCWWGG 3 cut(s) 1148, 1431, 1917
Eco147I AGGCCT 1 cut(s) 1916
Eco31I GGTCTC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 1305
Eco47I GGWCC 4 cut(s) 802, 1015, 1095, 2056
Eco57I CTGAAG 5 cut(s) 425, 771, 804, 2249, 2338
Eco81I CCTNAGG 1 cut(s) 1936
Eco88I CYCGRG 1 cut(s) 1400
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoO109I RGGNCCY 1 cut(s) 1095
EcoRI GAATTC 2 cut(s) 284, 710
EcoRII CCWGG 1 cut(s) 2286
EcoRV GATATC 1 cut(s) 1305
EcoT14I CCWWGG 3 cut(s) 1148, 1431, 1917
ErhI CCWWGG 3 cut(s) 1148, 1431, 1917
Esp3I CGTCTC 1 cut(s) 700
FaeI CATG 9 cut(s) 292, 535, 718, 869, 1319, 1361, 1455, 1718, 1805
FaqI GGGAC 4 cut(s) 737, 1001, 1954, 2264
FatI CATG 9 cut(s) 288, 531, 714, 865, 1315, 1357, 1451, 1714, 1801
FbaI TGATCA 2 cut(s) 78, 862
FokI GGATG 6 cut(s) 643, 833, 1270, 1516, 1886, 2083
FspI TGCGCA 1 cut(s) 1356
GlaI GCGC 3 cut(s) 1356, 1622, 2126
GsaI CCCAGC 1 cut(s) 2028
GsuI CTGGAG 3 cut(s) 1359, 1359, 1544
HaeIII GGCC 3 cut(s) 1197, 1916, 2084
HapII CCGG 1 cut(s) 1401
HgaI GACGC 1 cut(s) 739
HhaI GCGC 3 cut(s) 1357, 1623, 2127
Hin1I GRCGYC 2 cut(s) 528, 750
Hin1II CATG 9 cut(s) 292, 535, 718, 869, 1319, 1361, 1455, 1718, 1805
Hin6I GCGC 3 cut(s) 1355, 1621, 2125
HinP1I GCGC 3 cut(s) 1355, 1621, 2125
HincII GTYRAC 3 cut(s) 208, 1521, 2077
HindII GTYRAC 3 cut(s) 208, 1521, 2077
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 7 cut(s) 31, 1037, 1238, 1633, 1942, 2177, 2359
HpaII CCGG 1 cut(s) 1401
HphI GGTGA 4 cut(s) 4, 95, 350, 1197
Hpy166II GTNNAC 7 cut(s) 103, 208, 341, 1160, 1521, 2077, 2109
Hpy8I GTNNAC 7 cut(s) 103, 208, 341, 1160, 1521, 2077, 2109
Hpy99I CGWCG 1 cut(s) 1672
HpyAV CCTTC 9 cut(s) 139, 546, 771, 929, 1439, 2138, 2243, 2264, 2363
HpyCH4III ACNGT 9 cut(s) 26, 86, 222, 402, 449, 934, 1076, 1215, 2220
HpyCH4IV ACGT 2 cut(s) 528, 1116
HpyF10VI GCNNNNNNNGC 9 cut(s) 451, 1250, 1280, 1448, 1724, 1769, 1913, 1970, 2096
HpyF3I CTNAG 6 cut(s) 430, 496, 1936, 1946, 2301, 2345
HpySE526I ACGT 2 cut(s) 528, 1116
Hsp92I GRCGYC 2 cut(s) 528, 750
Hsp92II CATG 9 cut(s) 292, 535, 718, 869, 1319, 1361, 1455, 1718, 1805
HspAI GCGC 3 cut(s) 1355, 1621, 2125
Ksp22I TGATCA 2 cut(s) 78, 862
Kzo9I GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
LmnI GCTCC 3 cut(s) 275, 1826, 2273
Lsp1109I GCAGC 7 cut(s) 761, 1240, 1473, 1831, 1859, 1951, 2350
LweI GCATC 7 cut(s) 71, 752, 1021, 1292, 1538, 1951, 2047
MaeII ACGT 2 cut(s) 528, 1116
MaeIII GTNAC 4 cut(s) 1671, 1720, 1820, 2348
MalI GATC 8 cut(s) 80, 669, 812, 841, 864, 1510, 1791, 1926
MboI GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
MfeI CAATTG 3 cut(s) 1785, 2133, 2225
MflI RGATCY 2 cut(s) 839, 1508
MhlI GDGCHC 1 cut(s) 732
MlyI GAGTC 1 cut(s) 1936
MmeI TCCRAC 1 cut(s) 1090
MroXI GAANNNNTTC 1 cut(s) 1323
MseI TTAA 7 cut(s) 213, 323, 573, 675, 1290, 1547, 1746
MslI CAYNNNNRTG 3 cut(s) 705, 1292, 1320
MspA1I CMGCKG 2 cut(s) 774, 1486
MspI CCGG 1 cut(s) 1401
MspR9I CCNGG 3 cut(s) 1401, 1402, 2288
MunI CAATTG 3 cut(s) 1785, 2133, 2225
Mva1269I GAATGC 2 cut(s) 1181, 2151
MvaI CCWGG 1 cut(s) 2288
MvnI CGCG 1 cut(s) 2127
MwoI GCNNNNNNNGC 9 cut(s) 451, 1250, 1280, 1448, 1724, 1769, 1913, 1970, 2096
NciI CCSGG 2 cut(s) 1401, 1402
NdeII GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
NlaIII CATG 9 cut(s) 292, 535, 718, 869, 1319, 1361, 1455, 1718, 1805
NlaIV GGNNCC 5 cut(s) 804, 1017, 1828, 2058, 2185
NmuCI GTSAC 3 cut(s) 1720, 1820, 2348
NsbI TGCGCA 1 cut(s) 1356
NspI RCATGY 1 cut(s) 1718
PaeI GCATGC 1 cut(s) 1718
PceI AGGCCT 1 cut(s) 1916
PcsI WCGNNNNNNNCGW 1 cut(s) 645
PctI GAATGC 2 cut(s) 1181, 2151
PdmI GAANNNNTTC 1 cut(s) 1323
PfeI GAWTC 6 cut(s) 31, 1037, 1238, 1633, 2177, 2359
PflMI CCANNNNNTGG 1 cut(s) 685
PleI GAGTC 1 cut(s) 1936
PpsI GAGTC 1 cut(s) 1936
PpuMI RGGWCCY 1 cut(s) 1095
PshAI GACNNNNGTC 1 cut(s) 1165
PshBI ATTAAT 2 cut(s) 1547, 1746
Psp5II RGGWCCY 1 cut(s) 1095
Psp6I CCWGG 1 cut(s) 2286
PspFI CCCAGC 1 cut(s) 2024
PspGI CCWGG 1 cut(s) 2286
PspN4I GGNNCC 5 cut(s) 804, 1017, 1828, 2058, 2185
PspPI GGNCC 4 cut(s) 802, 1015, 1095, 2056
PspPPI RGGWCCY 1 cut(s) 1095
PstI CTGCAG 1 cut(s) 1849
PstNI CAGNNNCTG 1 cut(s) 1511
PsuI RGATCY 2 cut(s) 839, 1508
PvuII CAGCTG 1 cut(s) 1486
RsaI GTAC 3 cut(s) 171, 767, 2254
RsaNI GTAC 3 cut(s) 170, 766, 2253
RseI CAYNNNNRTG 3 cut(s) 705, 1292, 1320
SaqAI TTAA 7 cut(s) 213, 323, 573, 675, 1290, 1547, 1746
Sau3AI GATC 8 cut(s) 78, 667, 810, 839, 862, 1508, 1789, 1924
Sau96I GGNCC 4 cut(s) 802, 1015, 1095, 2056
SchI GAGTC 1 cut(s) 1936
ScrFI CCNGG 3 cut(s) 1401, 1402, 2288
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 7 cut(s) 71, 752, 1021, 1292, 1538, 1951, 2047
SfcI CTRYAG 2 cut(s) 615, 1845
SinI GGWCC 4 cut(s) 802, 1015, 1095, 2056
SmaI CCCGGG 1 cut(s) 1402
SmiMI CAYNNNNRTG 3 cut(s) 705, 1292, 1320
SpeI ACTAGT 2 cut(s) 1247, 2161
SphI GCATGC 1 cut(s) 1718
SseBI AGGCCT 1 cut(s) 1916
SsiI CCGC 6 cut(s) 384, 772, 1592, 1606, 2117, 2208
StuI AGGCCT 1 cut(s) 1916
StyD4I CCNGG 3 cut(s) 1399, 1400, 2286
StyI CCWWGG 3 cut(s) 1148, 1431, 1917
TaaI ACNGT 9 cut(s) 26, 86, 222, 402, 449, 934, 1076, 1215, 2220
TaiI ACGT 2 cut(s) 531, 1119
TaqI TCGA 8 cut(s) 94, 125, 311, 564, 639, 1085, 1091, 1636
TaqII GACCGA 1 cut(s) 1887
TatI WGTACW 2 cut(s) 169, 2252
TauI GCSGC 4 cut(s) 387, 1595, 1609, 2210
TfiI GAWTC 6 cut(s) 31, 1037, 1238, 1633, 2177, 2359
Tru1I TTAA 7 cut(s) 213, 323, 573, 675, 1290, 1547, 1746
Tru9I TTAA 7 cut(s) 213, 323, 573, 675, 1290, 1547, 1746
TscAI CASTG 3 cut(s) 452, 1281, 1658
TseFI GTSAC 3 cut(s) 1720, 1820, 2348
TseI GCWGC 7 cut(s) 774, 1253, 1486, 1844, 1847, 1964, 2338
Tsp45I GTSAC 3 cut(s) 1720, 1820, 2348
TspGWI ACGGA 3 cut(s) 1064, 1643, 1839
TspMI CCCGGG 1 cut(s) 1400
TspRI CASTG 3 cut(s) 452, 1281, 1658
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 4 cut(s) 802, 1015, 1095, 2056
VspI ATTAAT 2 cut(s) 1547, 1746
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 7 cut(s) 284, 318, 459, 710, 1735, 1837, 2101
XceI RCATGY 1 cut(s) 1718
XmaI CCCGGG 1 cut(s) 1400
XmaJI CCTAGG 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 1323
ZraI GACGTC 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.