Rroxscaffold_7G00180670

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005506
Physical Location & Seq
Forward (+)
19988334 .. 19993345
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180670.1

Sequence Viewer

Length: 2475 bp
ATGGACACAGCATCTATAAAATGTCTAATCAATAGCATATCTCGATTGGTTCTTCTAGTTTCATGCCAATCAGTGAAGTCTGTGCCTATTCAGAAGGTTTACAGAACCATAGCTGATGTATTGAAGCTTTTGAAGCCGTTGCTTGATGAAGTAGTTGATTACAATATACCTTCAGATGACATTCTATTTCAAGAGTGTGAAGAATTGGATATGGCTGTCAATGAAGCTAGGGAATTCATGGAAAATTGGTCTCCAAAGTCGAGCAAACTATTAAGTGCTTTGCGGAGTGAGCCATTGTTGATAAAGATACAAAATTTATCACTCAAGATTTGCTGTACATTAAGTAGATTATTACAGTCATCTTCATCTGCTTCAGGTTTAATGGGTCTACAGGAAATTGACCGTTTGAAGCCTGAAAGAGTAACAGAATACTTAGAAGAGGCTCTGAAAAGTCAAACAAAAGATTTCATGCCTTCCATCAAATATCTTATGAAAATTACTGAATTGCTTAGCTTGACTTCCAGCCAAGAACTGTTGAAAGAAAGTATTGCTGTAGAGAAGGAAAGAATTACTGTTGAAGTTCGTGATGTTAGAGGGGAACTAGATCAAATCAATCAAATTGTGATTCTTGTCTCTCATATTCGCGAATTCATGGTTAAAAGTGACCGCATTACCGCATCTGGCATCCCGGTCCCTCCATACTTCCGCTGCCCTTTGTCTTCAGATCTCATTTTGGATCCTGTGATTGTAGCTTCGGGTCAAACATATGAGAGAACTGCCATACAAAAATGGCTTGATCATGGGTTGATTATCTGCCCAATGACCCGTCAGAGGCTGACACATACAAACCTCATTCCCAATTACTCATTCAAAGTTATGGTAGAAAGTTGGTGTCAAGAAAATAATATTAAACTTGCGACGAACTCTGACTGCACTAATGCTATCACTGTCCCATCTCCATTAGGTCACATCTCTCCTGAAGGTTCAATTCACACAGGTACCCAGTACTCCATCCACAGTAGCAATTCGACATCAAGATCCTCTGCTGAAGTTGAGAAGCAAAGAAAAGATGTATCTCCTAGATTGAGTGGAGAAAAGTCCAATGGACGCCCTAGCGGTGAGAAAGACCAGTCATCACCAAAACAGTCATATATTCATAGCAGGAGTGAATCAGCCTCAAGTACTGTTTCCAGTGTTGATTATATGCCTCCAGCATCTATTAAGGTGTCAGGGATATCCAATAAGCATGAAAAGGATAATGAGGTTTCTGGAGAAATTACATCTGAGCATCCTGTTGCTTATATTCCAAATAAAGAATCAGGAATTTCCCCTGGGCTATCAGGAAAGAAGTCTCACCTTTCCAAAGCAAATGTAGAACTGGCATCATGGCATGACAGCCCTAATTACCCAAACAAACAGCTGCTTCCATTTTCTAGCTCAGAATCTGATGGGTCGACGACAGCATCCCATGTCATGAAATTAGTTGAAGACCTCAAGAGCCAATTGGACGAAGTGCAAACTAGGGCTGCGGAAGAATTGCGGCTTCTTGCAAAGCACAATATGGATAATCGCATTATTATAGGCCAATGTGGTGCTATCACACCCTTGCTTTCGCTGCTATATTCAGAGATGAAGTTAACGCAAGAGCATGCTGTGACAGCTCTTTTGAATTTATCAATTAATGAAGACAATAAGGCCATGATTGCAGAAGCAGGAGCAATTGAACCGCTCATCCATGTTCTGAAAACTGGGAATGATGGTGCCAAAGAAAATTCAGCTGCGGCTTTATTCAGCCTCTCTGTCTTAGATGATTACAAGGCAAAAATTGGCCGTTCTGGTGCAGTGAAGGCCTTGGTTGATCTTCTAGGCTCCGGGACTCTTAGAGGGAAGAAAGATGCTGCTACTGCACTGTTTAATCTCTCAATTTTTCATGAAAATAAGGCTCGCCTAGTTCAAGCTGGGGCAGTGAAGTATCTTATTGAGTTGATGGACCCTGACACAGGGATGGCTGATAAGGCTGTTGCTCTTCTGGCAAATTTGTCCACCATTGTAGAGGGGCGCTTAGCAATTGCACGGGGAGGAGGAATTCCATTACTAGTTGAGGTCGTGGAATCAGGCTCTCAGAGGGGAAAGGAAAACTCTGCCTCCATTCTGTTGCAACTGTGCCTTCATAGTCCAAAGTTTTGTACTATGGTTCTTCAGGAAGGAGCTGTCCCTCCCCTGGTCGCTTTATCTCAGTCTGGCAGTCCAAGAGCAAAGGAAAAGCAATCCAGAAAGAAGCTTTACACCTTTTGCCCTGGTGATAGAGACAATAACTTAGCATTAACAGGAGATGAATCCTTAATGTCAAAGGTTTGCAAGAAACCTCAGACATGCAGAACAGATGTGATATTGTCATGCACAGCAGCTTCTCAGTCACTTCCGGAATCAGAGAGAAGGGGCTATGGGGAAGGGAAAATAGAGACATTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

824

Amino Acids

90.1

Weight (kDa)

6.53

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 3 - 132 1.2e-46 PUB2-like, N-terminal domain
U-box PF04564 231 - 302 2.7e-22 U-box domain
ARM_ARMC5 PF24768 489 - 663 1.8e-09 ARMC5
ARM_PUB PF25598 491 - 756 1.2e-22 Plant U-box protein ARM repeat region
Arm PF00514 522 - 559 7.5e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 554 - 724 2.2e-12 ARMC5
Arm PF00514 561 - 600 3.7e-06 Armadillo/beta-catenin-like repeat
Arm PF00514 604 - 641 8e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 998
AccB1I GGYRCC 2 cut(s) 998, 1760
AccBSI CCGCTC 1 cut(s) 1729
AccI GTMKAC 2 cut(s) 388, 1454
AccII CGCG 1 cut(s) 645
AccIII TCCGGA 1 cut(s) 2422
AciI CCGC 9 cut(s) 283, 667, 675, 706, 1116, 1529, 1540, 1727, 1781
AclWI GGATC 3 cut(s) 731, 744, 1032
AcoI YGGCCR 1 cut(s) 1828
AcsI RAATTY 8 cut(s) 233, 313, 647, 1323, 1669, 1771, 2035, 2085
AcuI CTGAAG 6 cut(s) 156, 357, 705, 999, 1068, 2183
AcyI GRCGYC 1 cut(s) 1108
AfaI GTAC 5 cut(s) 337, 1000, 1007, 1183, 2188
AfiI CCNNNNNNNGG 3 cut(s) 831, 2000, 2221
AhlI ACTAGT 1 cut(s) 2095
AjnI CCWGG 3 cut(s) 1330, 2220, 2296
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AloI GAACNNNNNNTCC 2 cut(s) 1716, 1748
Alw26I GTCTC 5 cut(s) 255, 637, 1356, 2301, 2456
AlwI GGATC 3 cut(s) 731, 744, 1032
AlwNI CAGNNNCTG 2 cut(s) 835, 1445
Aor13HI TCCGGA 1 cut(s) 2422
AoxI GGCC 4 cut(s) 1582, 1695, 1828, 1848
ApeKI GCWGC 7 cut(s) 708, 1420, 1526, 1615, 1778, 1898, 2405
ApoI RAATTY 8 cut(s) 233, 313, 647, 1323, 1669, 1771, 2035, 2085
ArsI GACNNNNNNTTYG 2 cut(s) 1648, 1680
AseI ATTAAT 1 cut(s) 1680
Asp718I GGTACC 1 cut(s) 998
AspLEI GCGC 1 cut(s) 2061
AspS9I GGNCC 2 cut(s) 691, 1990
AsuC2I CCSGG 2 cut(s) 689, 1873
AsuHPI GGTGA 4 cut(s) 1128, 1130, 1346, 2312
AvaII GGWCC 2 cut(s) 691, 1990
BaeI ACNNNNGTAYC 2 cut(s) 982, 1015
BamHI GGATCC 1 cut(s) 736
BanI GGYRCC 2 cut(s) 998, 1760
BbsI GAAGAC 3 cut(s) 711, 1494, 1692
BbvI GCAGC 7 cut(s) 695, 1407, 1513, 1602, 1765, 1885, 2417
BccI CCATC 7 cut(s) 485, 961, 1019, 1442, 1751, 1981, 1999
BceAI ACGGC 2 cut(s) 121, 1815
BciT130I CCWGG 3 cut(s) 1332, 2222, 2298
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 2 cut(s) 689, 1873
BcoDI GTCTC 5 cut(s) 255, 637, 1356, 2301, 2456
BcuI ACTAGT 1 cut(s) 2095
BfmI CTRYAG 2 cut(s) 389, 552
BfoI RGCGCY 1 cut(s) 2062
BglII AGATCT 1 cut(s) 724
BisI GCNGC 9 cut(s) 709, 1421, 1527, 1541, 1616, 1779, 1782, 1899, 2406
BlpI GCTNAGC 2 cut(s) 509, 2062
BlsI GCNGC 9 cut(s) 710, 1422, 1528, 1542, 1617, 1780, 1783, 1900, 2407
BmcAI AGTACT 2 cut(s) 1007, 1183
Bme1390I CCNGG 5 cut(s) 689, 1332, 1873, 2222, 2298
Bme18I GGWCC 2 cut(s) 691, 1990
BmgT120I GGNCC 2 cut(s) 691, 1990
BmiI GGNNCC 6 cut(s) 693, 738, 1000, 1762, 1870, 1992
BmrFI CCNGG 5 cut(s) 689, 1332, 1873, 2222, 2298
BmrI ACTGGG 2 cut(s) 997, 1758
BmsI GCATC 8 cut(s) 20, 686, 693, 1223, 1297, 1391, 1472, 1885
BmuI ACTGGG 2 cut(s) 997, 1758
BpiI GAAGAC 3 cut(s) 711, 1494, 1692
BpmI CTGGAG 2 cut(s) 1194, 1290
Bpu1102I GCTNAGC 2 cut(s) 509, 2062
BpuEI CTTGAG 3 cut(s) 308, 1162, 1478
BpuMI CCSGG 2 cut(s) 689, 1873
BsaHI GRCGYC 1 cut(s) 1108
BsaI GGTCTC 1 cut(s) 255
BsaJI CCNNGG 5 cut(s) 1330, 1331, 1851, 2220, 2296
BsaWI WCCGGW 1 cut(s) 2422
BsaXI ACNNNNNCTCC 2 cut(s) 2129, 2159
Bsc4I CCNNNNNNNGG 3 cut(s) 831, 2000, 2221
Bse1I ACTGG 5 cut(s) 1003, 1129, 1191, 1383, 1753
BseAI TCCGGA 1 cut(s) 2422
BseBI CCWGG 3 cut(s) 1332, 2222, 2298
BseDI CCNNGG 5 cut(s) 1330, 1331, 1851, 2220, 2296
BseGI GGATG 6 cut(s) 684, 1011, 1288, 1463, 1731, 2010
BseLI CCNNNNNNNGG 3 cut(s) 831, 2000, 2221
BseMII CTCAG 6 cut(s) 1275, 1452, 2135, 2249, 2381, 2426
BseNI ACTGG 5 cut(s) 1003, 1129, 1191, 1383, 1753
BseRI GAGGAG 1 cut(s) 2094
BseXI GCAGC 7 cut(s) 695, 1407, 1513, 1602, 1765, 1885, 2417
BseYI CCCAGC 1 cut(s) 1958
BsgI GTGCAG 3 cut(s) 916, 1860, 1890
Bsh1236I CGCG 1 cut(s) 645
BshFI GGCC 4 cut(s) 1584, 1697, 1830, 1850
BshNI GGYRCC 2 cut(s) 998, 1760
BsiSI CCGG 3 cut(s) 689, 1872, 2423
BslFI GGGAC 4 cut(s) 677, 935, 1888, 2198
BslI CCNNNNNNNGG 3 cut(s) 831, 2000, 2221
BsmAI GTCTC 5 cut(s) 255, 637, 1356, 2301, 2456
BsmFI GGGAC 4 cut(s) 677, 935, 1888, 2198
BsnI GGCC 4 cut(s) 1584, 1697, 1830, 1850
Bso31I GGTCTC 1 cut(s) 255
Bsp13I TCCGGA 1 cut(s) 2422
Bsp1407I TGTACA 1 cut(s) 335
Bsp143I GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
Bsp1720I GCTNAGC 2 cut(s) 509, 2062
Bsp68I TCGCGA 1 cut(s) 645
BspACI CCGC 9 cut(s) 283, 667, 675, 706, 1116, 1529, 1540, 1727, 1781
BspANI GGCC 4 cut(s) 1584, 1697, 1830, 1850
BspCNI CTCAG 6 cut(s) 1276, 1451, 2134, 2248, 2380, 2425
BspEI TCCGGA 1 cut(s) 2422
BspFNI CGCG 1 cut(s) 645
BspHI TCATGA 2 cut(s) 1473, 1930
BspLI GGNNCC 6 cut(s) 693, 738, 1000, 1762, 1870, 1992
BspPI GGATC 3 cut(s) 731, 744, 1032
BspQI GCTCTTC 1 cut(s) 2031
BspT107I GGYRCC 2 cut(s) 998, 1760
BspTNI GGTCTC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 1729
BsrGI TGTACA 1 cut(s) 335
BsrI ACTGG 5 cut(s) 1003, 1129, 1191, 1383, 1753
BssECI CCNNGG 5 cut(s) 1330, 1331, 1851, 2220, 2296
BssMI GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
BssNI GRCGYC 1 cut(s) 1108
BssT1I CCWWGG 1 cut(s) 1851
Bst2UI CCWGG 3 cut(s) 1332, 2222, 2298
Bst6I CTCTTC 2 cut(s) 432, 2031
BstACI GRCGYC 1 cut(s) 1108
BstAUI TGTACA 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 1650, 1945
BstENI CCTNNNNNAGG 1 cut(s) 1998
BstF5I GGATG 6 cut(s) 684, 1011, 1288, 1463, 1731, 2010
BstFNI CGCG 1 cut(s) 645
BstH2I RGCGCY 1 cut(s) 2062
BstHHI GCGC 1 cut(s) 2061
BstKTI GATC 6 cut(s) 607, 727, 739, 799, 1040, 1861
BstMAI GTCTC 5 cut(s) 255, 637, 1356, 2301, 2456
BstMBI GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
BstMWI GCNNNNNNNGC 9 cut(s) 133, 289, 1615, 1658, 1703, 1847, 1904, 2015, 2030
BstNI CCWGG 3 cut(s) 1332, 2222, 2298
BstNSI RCATGY 2 cut(s) 1652, 2376
BstSCI CCNGG 5 cut(s) 687, 1330, 1871, 2220, 2296
BstSFI CTRYAG 2 cut(s) 389, 552
BstUI CGCG 1 cut(s) 645
BstV1I GCAGC 7 cut(s) 695, 1407, 1513, 1602, 1765, 1885, 2417
BstV2I GAAGAC 3 cut(s) 711, 1494, 1692
BstX2I RGATCY 3 cut(s) 724, 736, 1037
BstYI RGATCY 3 cut(s) 724, 736, 1037
BsuRI GGCC 4 cut(s) 1584, 1697, 1830, 1850
BtsCI GGATG 6 cut(s) 684, 1011, 1288, 1463, 1731, 2010
BtsI GCAGTG 2 cut(s) 1848, 1971
BtsIMutI CAGTG 6 cut(s) 78, 945, 1198, 1848, 1907, 1971
BtuMI TCGCGA 1 cut(s) 645
Cac8I GCNNGC 2 cut(s) 1650, 1945
CaiI CAGNNNCTG 2 cut(s) 835, 1445
CciI TCATGA 2 cut(s) 1473, 1930
CfoI GCGC 1 cut(s) 2061
Cfr13I GGNCC 2 cut(s) 691, 1990
CseI GACGC 1 cut(s) 1116
Csp6I GTAC 5 cut(s) 336, 999, 1006, 1182, 2187
CviQI GTAC 5 cut(s) 336, 999, 1006, 1182, 2187
DpnI GATC 6 cut(s) 606, 726, 738, 798, 1039, 1860
DpnII GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
EaeI YGGCCR 1 cut(s) 1828
Eam1104I CTCTTC 2 cut(s) 432, 2031
EarI CTCTTC 2 cut(s) 432, 2031
Eco130I CCWWGG 1 cut(s) 1851
Eco147I AGGCCT 1 cut(s) 1850
Eco31I GGTCTC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 1236
Eco47I GGWCC 2 cut(s) 691, 1990
Eco57I CTGAAG 6 cut(s) 156, 357, 705, 999, 1068, 2183
EcoNI CCTNNNNNAGG 1 cut(s) 1998
EcoRI GAATTC 3 cut(s) 233, 647, 2085
EcoRII CCWGG 3 cut(s) 1330, 2220, 2296
EcoRV GATATC 1 cut(s) 1236
EcoT14I CCWWGG 1 cut(s) 1851
ErhI CCWWGG 1 cut(s) 1851
FaqI GGGAC 4 cut(s) 677, 935, 1888, 2198
FauNDI CATATG 1 cut(s) 766
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 2 cut(s) 388, 1454
Fnu4HI GCNGC 9 cut(s) 709, 1421, 1527, 1541, 1616, 1779, 1782, 1899, 2406
FokI GGATG 6 cut(s) 671, 998, 1275, 1450, 1718, 2017
Fsp4HI GCNGC 9 cut(s) 709, 1421, 1527, 1541, 1616, 1779, 1782, 1899, 2406
GlaI GCGC 1 cut(s) 2060
GluI GCNGC 9 cut(s) 709, 1421, 1527, 1541, 1616, 1779, 1782, 1899, 2406
GsaI CCCAGC 1 cut(s) 1962
GsuI CTGGAG 2 cut(s) 1194, 1290
HaeII RGCGCY 1 cut(s) 2062
HaeIII GGCC 4 cut(s) 1584, 1697, 1830, 1850
HapII CCGG 3 cut(s) 689, 1872, 2423
HgaI GACGC 1 cut(s) 1116
HhaI GCGC 1 cut(s) 2061
Hin1I GRCGYC 1 cut(s) 1108
Hin6I GCGC 1 cut(s) 2059
HinP1I GCGC 1 cut(s) 2059
HincII GTYRAC 2 cut(s) 1455, 1638
HindII GTYRAC 2 cut(s) 1455, 1638
HindIII AAGCTT 2 cut(s) 125, 2279
HinfI GANTC 8 cut(s) 625, 1169, 1316, 1442, 1876, 2111, 2336, 2426
HpaI GTTAAC 1 cut(s) 1638
HpaII CCGG 3 cut(s) 689, 1872, 2423
HphI GGTGA 4 cut(s) 1128, 1130, 1346, 2312
Hpy166II GTNNAC 5 cut(s) 100, 389, 1455, 1638, 2043
Hpy8I GTNNAC 5 cut(s) 100, 389, 1455, 1638, 2043
Hpy99I CGWCG 2 cut(s) 922, 1459
HpyF10VI GCNNNNNNNGC 9 cut(s) 133, 289, 1615, 1658, 1703, 1847, 1904, 2015, 2030
Hsp92I GRCGYC 1 cut(s) 1108
HspAI GCGC 1 cut(s) 2059
Kpn2I TCCGGA 1 cut(s) 2422
KpnI GGTACC 1 cut(s) 1002
Ksp22I TGATCA 1 cut(s) 796
KspAI GTTAAC 1 cut(s) 1638
Kzo9I GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
LguI GCTCTTC 1 cut(s) 2031
LmnI GCTCC 3 cut(s) 1715, 1874, 2207
Lsp1109I GCAGC 7 cut(s) 695, 1407, 1513, 1602, 1765, 1885, 2417
LweI GCATC 8 cut(s) 20, 686, 693, 1223, 1297, 1391, 1472, 1885
MaeIII GTNAC 5 cut(s) 421, 662, 965, 1654, 2415
MalI GATC 6 cut(s) 606, 726, 738, 798, 1039, 1860
MbiI CCGCTC 1 cut(s) 1729
MboI GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
MfeI CAATTG 3 cut(s) 1502, 1719, 2067
MflI RGATCY 3 cut(s) 724, 736, 1037
MlyI GAGTC 1 cut(s) 1870
MroI TCCGGA 1 cut(s) 2422
MslI CAYNNNNRTG 1 cut(s) 2003
MspA1I CMGCKG 3 cut(s) 708, 1420, 1778
MspI CCGG 3 cut(s) 689, 1872, 2423
MspR9I CCNGG 5 cut(s) 689, 1332, 1873, 2222, 2298
MunI CAATTG 3 cut(s) 1502, 1719, 2067
MvaI CCWGG 3 cut(s) 1332, 2222, 2298
MvnI CGCG 1 cut(s) 645
MwoI GCNNNNNNNGC 9 cut(s) 133, 289, 1615, 1658, 1703, 1847, 1904, 2015, 2030
NciI CCSGG 2 cut(s) 689, 1873
NdeI CATATG 1 cut(s) 766
NdeII GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
NlaIV GGNNCC 6 cut(s) 693, 738, 1000, 1762, 1870, 1992
NmuCI GTSAC 4 cut(s) 662, 965, 1654, 2415
NruI TCGCGA 1 cut(s) 645
NspI RCATGY 2 cut(s) 1652, 2376
PaeI GCATGC 1 cut(s) 1652
PagI TCATGA 2 cut(s) 1473, 1930
PasI CCCWGGG 1 cut(s) 1331
PceI AGGCCT 1 cut(s) 1850
PciSI GCTCTTC 1 cut(s) 2031
PfeI GAWTC 7 cut(s) 625, 1169, 1316, 1442, 2111, 2336, 2426
PfoI TCCNGGA 1 cut(s) 1871
PkrI GCNGC 9 cut(s) 710, 1422, 1528, 1542, 1617, 1780, 1783, 1900, 2407
PleI GAGTC 1 cut(s) 1870
PpsI GAGTC 1 cut(s) 1870
PshBI ATTAAT 1 cut(s) 1680
Psp6I CCWGG 3 cut(s) 1330, 2220, 2296
PspFI CCCAGC 1 cut(s) 1958
PspGI CCWGG 3 cut(s) 1330, 2220, 2296
PspN4I GGNNCC 6 cut(s) 693, 738, 1000, 1762, 1870, 1992
PspPI GGNCC 2 cut(s) 691, 1990
PstNI CAGNNNCTG 2 cut(s) 835, 1445
PsuI RGATCY 3 cut(s) 724, 736, 1037
PvuII CAGCTG 2 cut(s) 1420, 1778
RruI TCGCGA 1 cut(s) 645
RsaI GTAC 5 cut(s) 337, 1000, 1007, 1183, 2188
RsaNI GTAC 5 cut(s) 336, 999, 1006, 1182, 2187
RseI CAYNNNNRTG 1 cut(s) 2003
SalI GTCGAC 1 cut(s) 1453
SapI GCTCTTC 1 cut(s) 2031
SatI GCNGC 9 cut(s) 709, 1421, 1527, 1541, 1616, 1779, 1782, 1899, 2406
Sau3AI GATC 6 cut(s) 604, 724, 736, 796, 1037, 1858
Sau96I GGNCC 2 cut(s) 691, 1990
ScaI AGTACT 2 cut(s) 1007, 1183
SchI GAGTC 1 cut(s) 1870
ScrFI CCNGG 5 cut(s) 689, 1332, 1873, 2222, 2298
SfaNI GCATC 8 cut(s) 20, 686, 693, 1223, 1297, 1391, 1472, 1885
SfcI CTRYAG 2 cut(s) 389, 552
SinI GGWCC 2 cut(s) 691, 1990
SmiMI CAYNNNNRTG 1 cut(s) 2003
SmlI CTYRAG 3 cut(s) 323, 1177, 1493
SmoI CTYRAG 3 cut(s) 323, 1177, 1493
SpeI ACTAGT 1 cut(s) 2095
SphI GCATGC 1 cut(s) 1652
SseBI AGGCCT 1 cut(s) 1850
SsiI CCGC 9 cut(s) 283, 667, 675, 706, 1116, 1529, 1540, 1727, 1781
SspI AATATT 1 cut(s) 907
StuI AGGCCT 1 cut(s) 1850
StyD4I CCNGG 5 cut(s) 687, 1330, 1871, 2220, 2296
StyI CCWWGG 1 cut(s) 1851
TaqI TCGA 4 cut(s) 43, 260, 1028, 1454
TatI WGTACW 4 cut(s) 335, 1005, 1181, 2186
TauI GCSGC 2 cut(s) 1543, 1784
TfiI GAWTC 7 cut(s) 625, 1169, 1316, 1442, 2111, 2336, 2426
TscAI CASTG 6 cut(s) 78, 952, 1198, 1848, 1914, 1971
TseFI GTSAC 4 cut(s) 662, 965, 1654, 2415
TseI GCWGC 7 cut(s) 708, 1420, 1526, 1615, 1778, 1898, 2405
Tsp45I GTSAC 4 cut(s) 662, 965, 1654, 2415
TspRI CASTG 6 cut(s) 78, 952, 1198, 1848, 1914, 1971
VpaK11BI GGWCC 2 cut(s) 691, 1990
VspI ATTAAT 1 cut(s) 1680
XagI CCTNNNNNAGG 1 cut(s) 1998
XapI RAATTY 8 cut(s) 233, 313, 647, 1323, 1669, 1771, 2035, 2085
XceI RCATGY 2 cut(s) 1652, 2376
XmiI GTMKAC 2 cut(s) 388, 1454
ZrmI AGTACT 2 cut(s) 1007, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.