MD10G1183200.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005506
Physical Location & Seq
Reverse (-)
27636809 .. 27641759
4951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1183200.v1.1.491

Sequence Viewer

Length: 2370 bp
ATGAATAGAGGTGATATGGACACAGCATCTATGAAATGTCTGATAAACAGTATATCTCGATTCGTTCATCTAGTTTCAAGCCAAACATTGAAACATATTCCTATTCAGAAGGATTACAGAACCATAGTTGATGTACTGAAGCTTTTGAAGCCATTGATTGATGAAGTAGTCGACTTTAAAGTACCGTCAGATGACATTCTATATAGAGAGTGTGAAGAATTAGATATGGCTGTCAATAGAGCTAGGGAATTCATCGAAAACTGGTCTCCAATGTCGAGCAAAATTTCAAGTGCTTGGCGGTGTGAGCCGCTGTTGATAACCATCCAAAACTCTTCACTCAAGATTTGCAGCATATTACATAGACTGTCACAGTCATCTTCATCGGGGTCAAGTTTAACGGGTCTTCAGCACTGTACACAGGAAATTCAATCTTTGAAACTGGAAAGAGTAACAGAATACTTAGAAGAGGCTGTGAGAAGTCAAAGTAAAGATGTCATGCCTTCCACCAAACTTCTTACGAAAATTATCGAATTGCTTAATCTGACTTCAAACCAAGAACTGCTGAAAGAAAGCATTGCTGTAGAAAAGGAAAGAATGAATGTCGAAGTCAGTGATGTTAGAGGGAAATTAGATCAAATTAACCAAATTGTGGTTCTTGTCTCCCATATTCGTGAATTCATGGTCAAAATTGAGGGCCTTGAAACCGTATCTGGCGTCCCGATCCCTTCATACTTCCGCTGCGCTCTGTCTTCAGAACTCATGCTGGACCCTGTGATTGTAGCTTCAGGTCAAACCTATGAGAGATCTTCCATCCAAAAATGGATTGATCATGGGCTAACTATTTGCCCAAAAACCCGACAGAGGCTGTCCCATACTAATCTCATTCCCAATTATACTGTCAAAGCTATGATAGAAAGCTGGTGTCAGGAAAATGATATAAAACTTACGGCGAACTCTGACGGCTCTAATGCTATCTCGGTCCCTTCTCCATCATATCAAGTATCTCCTGCAGATTCAACTCACACAGATAGTTTGCATTGCTCTACACACAGTAGCAATTCGACATCGAGGTCATCTCTTGAAGTTGGAAACATATTTGAGAAGCAAAAGAAAGATGTCTCTCCTAGATTAGGTGGAGAAAAGTCCAATGGATGCGATAGCAACGAGAAAGGCCACTCATCACCAAAACAGTCATATAGTCATAGCAGGGGTGAATCAGCCACTAGTGCTGTTTCCAGTATTGATTATGCACCTCCAGCATCGATTAAGGTGTCAGGGATATCTAATAAGCATGAAAATGTTCGTAGGTTGTCTGGAGAAATCACATCGGAGCATCCGGTTGCTTCTACTCCAAGGAAACAATCAGCAATTCCCCCTGGGTTATCAGGAAAATTATGTCACATTTCCAAGACAAAAGCAGAAGGGGCATGGACTGATAGCCCCAGTTATCCTGATAGGCAGCTGCTTCCATTTTCTAGTTCAGGATCCGATGAGTTGACAACAGCATCCCATGTCAGGAATTTAATTGAAGACCTCAAGAGCCAGTCAAATGAAGTGCAAACTATGGCAGCAGAAGAGTTGCGGCTTCTTGGAAAGCACAATATGGAGAATCGAACTATTATAGGCCAGTGTGGTGCTATCACGCCATTACTTTCACTTCTATATTCAGGACAGTTGACAACACAAGAGCACGCTGTGACAGCTCTTTTGAATTTATCAATTAATGAAGACAACAAAGCGATGATTGCAGAAGCAGGGGCAATTGATCCCCTAATCCATGTTCTAAAAACAGGAAGTGACGGAGCGAAAGAAAATTCTGCTGCAACTTTGTTCAGCCTCTCTGTGTTGGACGAATACAAGGCAAAAATTGGTCGGTCTGGTGCAGTTAAGGCCTTGGTTGATCTTCTAGCCTCAGGGACTCTTCGGGGGAAGAAAGATGCTGGTACTGCATTGTTTAATCTGTCAATTTTTCATGAAAATAAAGCTCGCCTAGTTCAAGCTGGGGCTGTGAAGCATCTTATTGATTTGATGGACCCTGATTCTGGGATGGTTGACAAGGCCGTTGCTCTCCTGGCAAATTTGTCCACCATTGCAGAGGGCCGCATAGCAATTACACGGGAAGGGGGCATTCCGTTACTAGTTGAGATTGTTGAATCAGGTTCCCATAGAGGAAAGGAGAATGCTGCCTCCGTATTGTTGCAGTTGTGCCTTCATAGTCCAAAGTTTTGCACCCTCGTTCTTCAGGAAGGAGCTGTCCCTCCCCTGGTTGCCTTATCTCAGTCTGGCACACCACGAGCAAAGGAAAAGGCACAGCAGCTTCTCAGTCACTTCAGGAGTCAGCGAGAAGGGGCTATGGGAAAGGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

790

Amino Acids

85.94

Weight (kDa)

6.93

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 8 - 145 1.5e-50 PUB2-like, N-terminal domain
U-box PF04564 241 - 312 2e-23 U-box domain
ARM_ARMC5 PF24768 496 - 678 3.4e-11 ARMC5
ARM_PUB PF25598 505 - 779 3.9e-26 Plant U-box protein ARM repeat region
Arm PF00514 536 - 573 3.1e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 568 - 738 2.6e-12 ARMC5
Arm PF00514 575 - 614 1e-05 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1069
AccB7I CCANNNNNTGG 1 cut(s) 649
AccI GTMKAC 1 cut(s) 171
AciI CCGC 5 cut(s) 298, 308, 736, 1582, 2101
AclWI GGATC 4 cut(s) 715, 1479, 1492, 1760
AcsI RAATTY 8 cut(s) 248, 282, 423, 674, 1519, 1711, 1813, 2077
AcuI CTGAAG 6 cut(s) 158, 389, 735, 768, 2225, 2314
AcyI GRCGYC 1 cut(s) 714
AdeI CACNNNGTG 1 cut(s) 1696
AfaI GTAC 4 cut(s) 135, 183, 415, 1945
AfiI CCNNNNNNNGG 6 cut(s) 649, 861, 1130, 1515, 2042, 2263
AhlI ACTAGT 2 cut(s) 1223, 2137
AjnI CCWGG 3 cut(s) 1375, 2070, 2262
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
Alw21I GWGCWC 1 cut(s) 1692
Alw26I GTCTC 3 cut(s) 270, 664, 1123
AlwI GGATC 4 cut(s) 715, 1479, 1492, 1760
AlwNI CAGNNNCTG 1 cut(s) 865
AoxI GGCC 6 cut(s) 694, 1171, 1624, 1890, 2058, 2098
ApeKI GCWGC 8 cut(s) 348, 738, 1459, 1462, 1568, 1820, 2183, 2314
ApoI RAATTY 8 cut(s) 248, 282, 423, 674, 1519, 1711, 1813, 2077
ArsI GACNNNNNNTTYG 2 cut(s) 1690, 1722
AseI ATTAAT 1 cut(s) 1722
Asp700I GAANNNNTTC 1 cut(s) 1299
AspLEI GCGC 1 cut(s) 743
AspS9I GGNCC 5 cut(s) 694, 766, 979, 2032, 2098
AsuHPI GGTGA 3 cut(s) 23, 1173, 1223
AvaII GGWCC 3 cut(s) 766, 979, 2032
AxyI CCTNAGG 1 cut(s) 1912
BamHI GGATCC 1 cut(s) 1484
BauI CACGAG 1 cut(s) 2292
BbsI GAAGAC 4 cut(s) 395, 741, 1536, 1734
Bbv12I GWGCWC 1 cut(s) 1692
BbvI GCAGC 8 cut(s) 360, 725, 1449, 1471, 1580, 1807, 2170, 2326
BccI CCATC 5 cut(s) 329, 818, 997, 2023, 2041
BceAI ACGGC 3 cut(s) 963, 976, 2045
BciT130I CCWGG 3 cut(s) 1377, 2072, 2264
BclI TGATCA 1 cut(s) 826
BcoDI GTCTC 3 cut(s) 270, 664, 1123
BcuI ACTAGT 2 cut(s) 1223, 2137
BfaI CTAG 8 cut(s) 71, 243, 1125, 1224, 1476, 1907, 1991, 2138
BfmI CTRYAG 2 cut(s) 579, 1008
BglII AGATCT 1 cut(s) 803
Bme1390I CCNGG 3 cut(s) 1377, 2072, 2264
Bme18I GGWCC 3 cut(s) 766, 979, 2032
BmgT120I GGNCC 5 cut(s) 694, 766, 979, 2032, 2098
BmiI GGNNCC 5 cut(s) 768, 981, 1486, 2034, 2161
BmrFI CCNGG 3 cut(s) 1377, 2072, 2264
BmrI ACTGGG 1 cut(s) 1437
BmsI GCATC 7 cut(s) 35, 1142, 1268, 1342, 1514, 1927, 2023
BmuI ACTGGG 1 cut(s) 1437
BpiI GAAGAC 4 cut(s) 395, 741, 1536, 1734
BplI GAGNNNNNCTC 2 cut(s) 1060, 1092
BpmI CTGGAG 2 cut(s) 1239, 1335
BpuEI CTTGAG 2 cut(s) 323, 1520
Bsa29I ATCGAT 1 cut(s) 1262
BsaBI GATNNNNATC 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 5 cut(s) 1352, 1375, 1376, 1893, 2262
BsaWI WCCGGW 1 cut(s) 1336
Bsc4I CCNNNNNNNGG 6 cut(s) 649, 861, 1130, 1515, 2042, 2263
Bse1I ACTGG 6 cut(s) 266, 444, 1236, 1443, 1543, 1627
Bse21I CCTNAGG 1 cut(s) 1912
Bse3DI GCAATG 3 cut(s) 573, 1036, 2088
Bse8I GATNNNNATC 1 cut(s) 320
BseBI CCWGG 3 cut(s) 1377, 2072, 2264
BseCI ATCGAT 1 cut(s) 1262
BseDI CCNNGG 5 cut(s) 1352, 1375, 1376, 1893, 2262
BseGI GGATG 6 cut(s) 321, 810, 1157, 1333, 1505, 2052
BseJI GATNNNNATC 1 cut(s) 320
BseLI CCNNNNNNNGG 6 cut(s) 649, 861, 1130, 1515, 2042, 2263
BseMI GCAATG 3 cut(s) 573, 1036, 2088
BseMII CTCAG 3 cut(s) 1926, 2291, 2335
BseNI ACTGG 6 cut(s) 266, 444, 1236, 1443, 1543, 1627
BseXI GCAGC 8 cut(s) 360, 725, 1449, 1471, 1580, 1807, 2170, 2326
BseYI CCCAGC 1 cut(s) 2000
BsgI GTGCAG 1 cut(s) 1902
BshFI GGCC 6 cut(s) 696, 1173, 1626, 1892, 2060, 2100
BshVI ATCGAT 1 cut(s) 1262
BsiHKAI GWGCWC 1 cut(s) 1692
BsiSI CCGG 1 cut(s) 1337
BslFI GGGAC 5 cut(s) 701, 853, 965, 1930, 2240
BslI CCNNNNNNNGG 6 cut(s) 649, 861, 1130, 1515, 2042, 2263
BsmAI GTCTC 3 cut(s) 270, 664, 1123
BsmFI GGGAC 5 cut(s) 701, 853, 965, 1930, 2240
BsmI GAATGC 2 cut(s) 2127, 2185
BsnI GGCC 6 cut(s) 696, 1173, 1626, 1892, 2060, 2100
Bso31I GGTCTC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 1692
Bsp1407I TGTACA 1 cut(s) 413
Bsp143I GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
BspACI CCGC 5 cut(s) 298, 308, 736, 1582, 2101
BspANI GGCC 6 cut(s) 696, 1173, 1626, 1892, 2060, 2100
BspCNI CTCAG 3 cut(s) 1925, 2290, 2334
BspDI ATCGAT 1 cut(s) 1262
BspHI TCATGA 1 cut(s) 1972
BspLI GGNNCC 5 cut(s) 768, 981, 1486, 2034, 2161
BspMAI CTGCAG 1 cut(s) 1012
BspPI GGATC 4 cut(s) 715, 1479, 1492, 1760
BspTNI GGTCTC 1 cut(s) 270
BsrDI GCAATG 3 cut(s) 573, 1036, 2088
BsrGI TGTACA 1 cut(s) 413
BsrI ACTGG 6 cut(s) 266, 444, 1236, 1443, 1543, 1627
BssECI CCNNGG 5 cut(s) 1352, 1375, 1376, 1893, 2262
BssMI GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
BssNI GRCGYC 1 cut(s) 714
BssSI CACGAG 1 cut(s) 2292
BssT1I CCWWGG 2 cut(s) 1352, 1893
Bst2BI CACGAG 1 cut(s) 2292
Bst2UI CCWGG 3 cut(s) 1377, 2072, 2264
Bst6I CTCTTC 4 cut(s) 337, 459, 1569, 1926
BstACI GRCGYC 1 cut(s) 714
BstAUI TGTACA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 1692, 1987
BstDEI CTNAG 4 cut(s) 460, 1912, 2277, 2321
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 6 cut(s) 321, 810, 1157, 1333, 1505, 2052
BstHHI GCGC 1 cut(s) 743
BstKTI GATC 7 cut(s) 634, 723, 806, 829, 1487, 1768, 1903
BstMAI GTCTC 3 cut(s) 270, 664, 1123
BstMBI GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
BstNI CCWGG 3 cut(s) 1377, 2072, 2264
BstSCI CCNGG 3 cut(s) 1375, 2070, 2262
BstSFI CTRYAG 2 cut(s) 579, 1008
BstV1I GCAGC 8 cut(s) 360, 725, 1449, 1471, 1580, 1807, 2170, 2326
BstV2I GAAGAC 4 cut(s) 395, 741, 1536, 1734
BstX2I RGATCY 2 cut(s) 803, 1484
BstYI RGATCY 2 cut(s) 803, 1484
Bsu15I ATCGAT 1 cut(s) 1262
Bsu36I CCTNAGG 1 cut(s) 1912
BsuRI GGCC 6 cut(s) 696, 1173, 1626, 1892, 2060, 2100
BsuTUI ATCGAT 1 cut(s) 1262
BtgZI GCGATG 1 cut(s) 1754
BtsCI GGATG 6 cut(s) 321, 810, 1157, 1333, 1505, 2052
BtsIMutI CAGTG 3 cut(s) 409, 616, 1634
Cac8I GCNNGC 2 cut(s) 1692, 1987
CaiI CAGNNNCTG 1 cut(s) 865
CciI TCATGA 1 cut(s) 1972
CfoI GCGC 1 cut(s) 743
Cfr13I GGNCC 5 cut(s) 694, 766, 979, 2032, 2098
ClaI ATCGAT 1 cut(s) 1262
CseI GACGC 1 cut(s) 703
Csp6I GTAC 4 cut(s) 134, 182, 414, 1944
CviAII CATG 9 cut(s) 496, 679, 760, 830, 1292, 1428, 1511, 1778, 1973
CviQI GTAC 4 cut(s) 134, 182, 414, 1944
DdeI CTNAG 4 cut(s) 460, 1912, 2277, 2321
DpnI GATC 7 cut(s) 633, 722, 805, 828, 1486, 1767, 1902
DpnII GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
DraI TTTAAA 1 cut(s) 178
DraIII CACNNNGTG 1 cut(s) 1696
DrdI GACNNNNNNGTC 1 cut(s) 1069
DseDI GACNNNNNNGTC 1 cut(s) 1069
Eam1104I CTCTTC 4 cut(s) 337, 459, 1569, 1926
EarI CTCTTC 4 cut(s) 337, 459, 1569, 1926
Eco130I CCWWGG 2 cut(s) 1352, 1893
Eco147I AGGCCT 1 cut(s) 1892
Eco31I GGTCTC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 1281
Eco47I GGWCC 3 cut(s) 766, 979, 2032
Eco57I CTGAAG 6 cut(s) 158, 389, 735, 768, 2225, 2314
Eco81I CCTNAGG 1 cut(s) 1912
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 1 cut(s) 694
EcoRI GAATTC 2 cut(s) 248, 674
EcoRII CCWGG 3 cut(s) 1375, 2070, 2262
EcoRV GATATC 1 cut(s) 1281
EcoT14I CCWWGG 2 cut(s) 1352, 1893
ErhI CCWWGG 2 cut(s) 1352, 1893
FaeI CATG 9 cut(s) 499, 682, 763, 833, 1295, 1431, 1514, 1781, 1976
FaqI GGGAC 5 cut(s) 701, 853, 965, 1930, 2240
FatI CATG 9 cut(s) 495, 678, 759, 829, 1291, 1427, 1510, 1777, 1972
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 171
FokI GGATG 6 cut(s) 308, 797, 1164, 1320, 1492, 2059
FspBI CTAG 8 cut(s) 71, 243, 1125, 1224, 1476, 1907, 1991, 2138
GlaI GCGC 1 cut(s) 742
GsaI CCCAGC 1 cut(s) 2004
GsuI CTGGAG 2 cut(s) 1239, 1335
HaeIII GGCC 6 cut(s) 696, 1173, 1626, 1892, 2060, 2100
HapII CCGG 1 cut(s) 1337
HgaI GACGC 1 cut(s) 703
HhaI GCGC 1 cut(s) 743
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 9 cut(s) 499, 682, 763, 833, 1295, 1431, 1514, 1781, 1976
Hin6I GCGC 1 cut(s) 741
HinP1I GCGC 1 cut(s) 741
HincII GTYRAC 4 cut(s) 172, 1497, 1677, 2053
HindII GTYRAC 4 cut(s) 172, 1497, 1677, 2053
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 8 cut(s) 60, 1013, 1214, 1609, 1918, 2039, 2153, 2335
HpaII CCGG 1 cut(s) 1337
HphI GGTGA 3 cut(s) 23, 1173, 1223
Hpy166II GTNNAC 6 cut(s) 172, 416, 1497, 1677, 2053, 2085
Hpy188I TCNGA 8 cut(s) 42, 108, 190, 543, 754, 958, 1330, 1489
Hpy8I GTNNAC 6 cut(s) 172, 416, 1497, 1677, 2053, 2085
HpyAV CCTTC 9 cut(s) 103, 510, 735, 993, 1415, 2114, 2219, 2240, 2339
HpyF3I CTNAG 4 cut(s) 460, 1912, 2277, 2321
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 9 cut(s) 499, 682, 763, 833, 1295, 1431, 1514, 1781, 1976
HspAI GCGC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
LmnI GCTCC 3 cut(s) 1330, 1802, 2249
Lsp1109I GCAGC 8 cut(s) 360, 725, 1449, 1471, 1580, 1807, 2170, 2326
LweI GCATC 7 cut(s) 35, 1142, 1268, 1342, 1514, 1927, 2023
MaeI CTAG 8 cut(s) 71, 243, 1125, 1224, 1476, 1907, 1991, 2138
MaeIII GTNAC 7 cut(s) 366, 448, 1397, 1696, 1796, 2133, 2324
MalI GATC 7 cut(s) 633, 722, 805, 828, 1486, 1767, 1902
MboI GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
MfeI CAATTG 1 cut(s) 1761
MflI RGATCY 2 cut(s) 803, 1484
MhlI GDGCHC 1 cut(s) 1692
MlyI GAGTC 2 cut(s) 1912, 2344
MmeI TCCRAC 2 cut(s) 1066, 1827
MroXI GAANNNNTTC 1 cut(s) 1299
MseI TTAA 9 cut(s) 177, 395, 537, 639, 1266, 1523, 1722, 1887, 1956
MslI CAYNNNNRTG 2 cut(s) 669, 1296
MspA1I CMGCKG 3 cut(s) 310, 738, 1462
MspI CCGG 1 cut(s) 1337
MspR9I CCNGG 3 cut(s) 1377, 2072, 2264
MunI CAATTG 1 cut(s) 1761
Mva1269I GAATGC 2 cut(s) 2127, 2185
MvaI CCWGG 3 cut(s) 1377, 2072, 2264
NdeII GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
NlaIII CATG 9 cut(s) 499, 682, 763, 833, 1295, 1431, 1514, 1781, 1976
NlaIV GGNNCC 5 cut(s) 768, 981, 1486, 2034, 2161
NmuCI GTSAC 5 cut(s) 366, 1397, 1696, 1796, 2324
PagI TCATGA 1 cut(s) 1972
PasI CCCWGGG 1 cut(s) 1376
PceI AGGCCT 1 cut(s) 1892
PctI GAATGC 2 cut(s) 2127, 2185
PdmI GAANNNNTTC 1 cut(s) 1299
PfeI GAWTC 6 cut(s) 60, 1013, 1214, 1609, 2039, 2153
PflMI CCANNNNNTGG 1 cut(s) 649
PleI GAGTC 2 cut(s) 1912, 2343
PpsI GAGTC 2 cut(s) 1912, 2343
PshBI ATTAAT 1 cut(s) 1722
Psp6I CCWGG 3 cut(s) 1375, 2070, 2262
PspFI CCCAGC 1 cut(s) 2000
PspGI CCWGG 3 cut(s) 1375, 2070, 2262
PspN4I GGNNCC 5 cut(s) 768, 981, 1486, 2034, 2161
PspPI GGNCC 5 cut(s) 694, 766, 979, 2032, 2098
PstI CTGCAG 1 cut(s) 1012
PstNI CAGNNNCTG 1 cut(s) 865
PsuI RGATCY 2 cut(s) 803, 1484
PvuII CAGCTG 1 cut(s) 1462
RsaI GTAC 4 cut(s) 135, 183, 415, 1945
RsaNI GTAC 4 cut(s) 134, 182, 414, 1944
RseI CAYNNNNRTG 2 cut(s) 669, 1296
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 9 cut(s) 177, 395, 537, 639, 1266, 1523, 1722, 1887, 1956
Sau3AI GATC 7 cut(s) 631, 720, 803, 826, 1484, 1765, 1900
Sau96I GGNCC 5 cut(s) 694, 766, 979, 2032, 2098
SchI GAGTC 2 cut(s) 1912, 2344
ScrFI CCNGG 3 cut(s) 1377, 2072, 2264
SduI GDGCHC 1 cut(s) 1692
SfaNI GCATC 7 cut(s) 35, 1142, 1268, 1342, 1514, 1927, 2023
SfcI CTRYAG 2 cut(s) 579, 1008
SinI GGWCC 3 cut(s) 766, 979, 2032
SmiMI CAYNNNNRTG 2 cut(s) 669, 1296
SmlI CTYRAG 2 cut(s) 338, 1535
SmoI CTYRAG 2 cut(s) 338, 1535
SpeI ACTAGT 2 cut(s) 1223, 2137
SseBI AGGCCT 1 cut(s) 1892
SsiI CCGC 5 cut(s) 298, 308, 736, 1582, 2101
SspMI CTAG 8 cut(s) 71, 243, 1125, 1224, 1476, 1907, 1991, 2138
StuI AGGCCT 1 cut(s) 1892
StyD4I CCNGG 3 cut(s) 1375, 2070, 2262
StyI CCWWGG 2 cut(s) 1352, 1893
TaqII GACCGA 2 cut(s) 967, 1863
TatI WGTACW 2 cut(s) 133, 413
TauI GCSGC 3 cut(s) 310, 1585, 2103
TfiI GAWTC 6 cut(s) 60, 1013, 1214, 1609, 2039, 2153
Tru1I TTAA 9 cut(s) 177, 395, 537, 639, 1266, 1523, 1722, 1887, 1956
Tru9I TTAA 9 cut(s) 177, 395, 537, 639, 1266, 1523, 1722, 1887, 1956
TscAI CASTG 3 cut(s) 416, 616, 1634
TseFI GTSAC 5 cut(s) 366, 1397, 1696, 1796, 2324
TseI GCWGC 8 cut(s) 348, 738, 1459, 1462, 1568, 1820, 2183, 2314
Tsp45I GTSAC 5 cut(s) 366, 1397, 1696, 1796, 2324
TspGWI ACGGA 3 cut(s) 1815, 2121, 2179
TspRI CASTG 3 cut(s) 416, 616, 1634
Van91I CCANNNNNTGG 1 cut(s) 649
VpaK11BI GGWCC 3 cut(s) 766, 979, 2032
VspI ATTAAT 1 cut(s) 1722
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 8 cut(s) 248, 282, 423, 674, 1519, 1711, 1813, 2077
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 8 cut(s) 71, 243, 1125, 1224, 1476, 1907, 1991, 2138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.