pycom05g18300

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005506
Physical Location & Seq
Reverse (-)
21284970 .. 21288189
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18300.1

Sequence Viewer

Length: 2145 bp
ATGGCTGTCAATGGAGCTAGAGAATTCATGGAAAAATGGTCTCCAAAGTCGAGCAAAGTTTTAAGTGCTTGGTGGAGTGAGCCATTGTTGATAACAATCCAAGGCTCGTCGCTCAAGATTTGCGGCATATTACCTAGACTGTTGCAGTCATCTTCATCTGGCTCAAGCTTAACAGGTCTTCAGCACTGTATGCAGGAAATTCAATCTTTGAAGCTGGAAAGAGTATCAGAATACTTAGAAGAGGCTCTGAAAAGTCCAAGGAAAGATGTCATGCCTTCCACCGAACTTCTTATGAAAATTATCGAATTGCTTAATCTGACTTCAAACCAAGAACTGCTGAAAGAAAGCATTGCTGTAGAAAAGGAAAGGATGAATGTCGAAGTCAGCGATGTTAGAGGGGAATTAGATCAAGTTAACCAAATTGTGGTTCTCGTCTCCCATATTCGTGAATTCATGGTCAAAATTGAGCACCTTGAAACTGCATCTGGCGTCCCAATCCCTTCGTACTTCCGCTGCCCTTTGTCTTCAGAACTAATGCTGGACCCTGTGATCGTAGCTTCAGGTCAAACCTATGAGAGATCTTCCATCCAAAAATGGATCGATCATGGGCTAACTATTTGCCCAAAGACCCGACAGAGGCTTTCCCATACAAATCTCCTTCCCAATTATACTGTCAAAGCTATGATAGAAAGCTGGTGTCAGGAAAATGATATAAAACTTACGGCGAACTCTGACGGCTCTAATGCTATCTTGGTCCCATCTCCATCAGATCAAGTATCTCCTGCAGATTCAATTCACACAGATAGTTTGTATTGCTCTACACACAGTAGCAATTCGACATCGAGGTCCTCTCTTGAAGTTGGAAACGTATTTGAGAAACAAAAGAAACATGTCTCTCCTAGGTTAGGTGGACAAAAGTCCAATGAATGCGATAGCAACAAGAAAGGCCACTCATCACCAAAACAGTCATATAGTCATTGCAGGAGCGAATCAGCTACTAGTGCTGCTTCCAGTATTGATTATGCACTGCCAGCATCCATTAAGGTGTCGGGGATATCTAATAAGCATGAAAATGTTCGTAGGTTGTCTGGAGAAATCTCATCTGAGCATGCAGTTGCTTCTACTCCAGATAAAGAATCAACAATGCCCCCCGGGTTATCAGGAAAATTATCTCACATTTCCAAGGCAAAAGCAGAAGGGGCATGGACTGACAGCCCCAGTTATCCCAACAGACAGCTGCTTCCATTTTCTAGTTCAGGATCTGATGAGTTGACGACAGCATCCCACGTCCGGAAATTAATTGAAGACCTCCAGAGCCAGTCAAATGAAGTGCAAACTATGGCGGCGGAAGAGTTGCGGCTTCTTGGGAAGCGCAATACGGAGAATCGAACTATGATAGGCCAGTGTGGTGCTATCACGCCATTACTTTCTCTTCTATATTCAGGAGTGATGATAACACAAGAGCATGCTGTGACAGCTCTTTTGAATTTATCGATTAATGAAGACAACAAAGCGATGATTGCGGAAGCAGGGGCAATTGATCCCCTAATCCATGTTCTAAAAACGGGAAGTGACGGAGCCAAAGAAAATTCTGCTGCAGCTTTGTTCAGCCTCTCTGTATTGGACGAATACAAGGCAAAAATTGGTCGGTCTGGTGCAGTTAAGGCCTTGATCGATCTTCTAGCCTCAGGGACTCTTAGAGGGAAGAAAGATGCTGCTACTGCATTGTTCAATCTGTCAATTTTTCACGAAAATAAAGCTCGCCTAGTTCAAGCTGGGGCTGTGAAGCATCTTATTGATTTGATGGACCCTGATACTGGGATGGTTGACAAGGCCGTTGCTCTCCTAGCAAATTTGTCCACCATTGCGGAGGGGCGCGTAGCAATTGCACGGGAAGGGGGCATTCCATTACTAGTCGAGATTGTTGAATCAGGTTCCCAGAGAGGAAAGGAAAATGCCGCCTCCATACTGTTGCAATTGTGCCTTCATAGTCCAAAGTTTTGTACTCTTGTTCTTCAGGAAGGAGCTGTCCCTCCCCTGGTTGCCTTATCTCAGTCGGGCACCCCAAGAGCAAAGGAAAAGGCACAGCAGCTTCTCAGTCACTTCAGGAATCAGCGAGAAGGGGCAATGGGAAAAGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

715

Amino Acids

77.33

Weight (kDa)

7.05

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 2 - 70 3.6e-18 PUB2-like, N-terminal domain
U-box PF04564 166 - 237 5.2e-24 U-box domain
ARM_ARMC5 PF24768 421 - 602 8.4e-12 ARMC5
ARM_PUB PF25598 430 - 704 5.6e-27 Plant U-box protein ARM repeat region
Arm PF00514 459 - 498 8.1e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 493 - 663 1.3e-13 ARMC5
Arm PF00514 500 - 539 4.3e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 844
AccB1I GGYRCC 1 cut(s) 2060
AccB7I CCANNNNNTGG 1 cut(s) 424
AccII CGCG 1 cut(s) 1878
AccIII TCCGGA 1 cut(s) 1290
AciI CCGC 8 cut(s) 123, 511, 1343, 1346, 1357, 1523, 1868, 1959
AclWI GGATC 3 cut(s) 605, 1267, 1535
AcsI RAATTY 6 cut(s) 23, 198, 449, 1486, 1588, 1852
AcuI CTGAAG 5 cut(s) 164, 510, 543, 2000, 2089
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 2 cut(s) 506, 2005
AfiI CCNNNNNNNGG 6 cut(s) 424, 636, 905, 1290, 1817, 2038
AflIII ACRYGT 1 cut(s) 889
AhlI ACTAGT 2 cut(s) 998, 1912
AjiI CACGTC 1 cut(s) 1288
AjnI CCWGG 1 cut(s) 2037
AjuI GAANNNNNNNTTGG 2 cut(s) 411, 443
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 3 cut(s) 45, 439, 898
AlwI GGATC 3 cut(s) 605, 1267, 1535
AlwNI CAGNNNCTG 1 cut(s) 1262
Ama87I CYCGRG 1 cut(s) 1151
Aor13HI TCCGGA 1 cut(s) 1290
AoxI GGCC 4 cut(s) 946, 1399, 1665, 1833
ApeKI GCWGC 7 cut(s) 513, 1004, 1237, 1595, 1598, 1715, 2089
ApoI RAATTY 6 cut(s) 23, 198, 449, 1486, 1588, 1852
ArsI GACNNNNNNTTYG 2 cut(s) 1465, 1497
AseI ATTAAT 2 cut(s) 1298, 1497
Asp700I GAANNNNTTC 1 cut(s) 1074
AspA2I CCTAGG 1 cut(s) 899
AspLEI GCGC 2 cut(s) 1374, 1878
AspS9I GGNCC 4 cut(s) 541, 754, 846, 1807
AsuC2I CCSGG 2 cut(s) 1152, 1153
AsuHPI GGTGA 1 cut(s) 948
AvaI CYCGRG 1 cut(s) 1151
AvaII GGWCC 4 cut(s) 541, 754, 846, 1807
AvrII CCTAGG 1 cut(s) 899
AxyI CCTNAGG 1 cut(s) 1687
BaeGI GKGCMC 1 cut(s) 2063
BanI GGYRCC 1 cut(s) 2060
BbsI GAAGAC 4 cut(s) 170, 516, 1311, 1509
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 7 cut(s) 500, 991, 1224, 1582, 1610, 1702, 2101
BccI CCATC 5 cut(s) 593, 766, 772, 1798, 1816
BceAI ACGGC 3 cut(s) 738, 751, 1820
BciT130I CCWGG 1 cut(s) 2039
BcnI CCSGG 2 cut(s) 1152, 1153
BcoDI GTCTC 3 cut(s) 45, 439, 898
BcuI ACTAGT 2 cut(s) 998, 1912
BfaI CTAG 9 cut(s) 18, 135, 900, 999, 1251, 1682, 1766, 1847, 1913
BfmI CTRYAG 3 cut(s) 354, 783, 1596
BglII AGATCT 1 cut(s) 578
BlnI CCTAGG 1 cut(s) 899
Bme1390I CCNGG 3 cut(s) 1152, 1153, 2039
Bme18I GGWCC 4 cut(s) 541, 754, 846, 1807
BmeT110I CYCGRG 1 cut(s) 1151
BmgBI CACGTC 1 cut(s) 1288
BmgT120I GGNCC 4 cut(s) 541, 754, 846, 1807
BmiI GGNNCC 6 cut(s) 543, 756, 1579, 1809, 1936, 2062
BmrFI CCNGG 3 cut(s) 1152, 1153, 2039
BmrI ACTGGG 2 cut(s) 1212, 1827
BmsI GCATC 5 cut(s) 491, 1043, 1289, 1702, 1798
BmuI ACTGGG 2 cut(s) 1212, 1827
BoxI GACNNNNGTC 1 cut(s) 916
BpiI GAAGAC 4 cut(s) 170, 516, 1311, 1509
BplI GAGNNNNNCTC 2 cut(s) 835, 867
BpmI CTGGAG 3 cut(s) 1110, 1110, 1295
BpuEI CTTGAG 2 cut(s) 98, 148
BpuMI CCSGG 2 cut(s) 1152, 1153
Bsa29I ATCGAT 3 cut(s) 600, 1493, 1674
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 7 cut(s) 100, 257, 899, 1150, 1151, 1182, 2037
BsaWI WCCGGW 1 cut(s) 1290
Bsc4I CCNNNNNNNGG 6 cut(s) 424, 636, 905, 1290, 1817, 2038
Bse1I ACTGG 5 cut(s) 1011, 1218, 1318, 1402, 1822
Bse21I CCTNAGG 1 cut(s) 1687
Bse3DI GCAATG 4 cut(s) 348, 976, 1863, 2133
Bse8I GATNNNNATC 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 1290
BseBI CCWGG 1 cut(s) 2039
BseCI ATCGAT 3 cut(s) 600, 1493, 1674
BseDI CCNNGG 7 cut(s) 100, 257, 899, 1150, 1151, 1182, 2037
BseGI GGATG 5 cut(s) 375, 585, 1034, 1280, 1827
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 6 cut(s) 424, 636, 905, 1290, 1817, 2038
BseMI GCAATG 4 cut(s) 348, 976, 1863, 2133
BseMII CTCAG 4 cut(s) 1095, 1701, 2066, 2110
BseNI ACTGG 5 cut(s) 1011, 1218, 1318, 1402, 1822
BseSI GKGCMC 1 cut(s) 2063
BseXI GCAGC 7 cut(s) 500, 991, 1224, 1582, 1610, 1702, 2101
BseYI CCCAGC 1 cut(s) 1775
BsgI GTGCAG 1 cut(s) 1677
Bsh1236I CGCG 1 cut(s) 1878
BshFI GGCC 4 cut(s) 948, 1401, 1667, 1835
BshNI GGYRCC 1 cut(s) 2060
BshVI ATCGAT 3 cut(s) 600, 1493, 1674
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 1151
BsiSI CCGG 2 cut(s) 1152, 1291
BslFI GGGAC 4 cut(s) 476, 740, 1705, 2015
BslI CCNNNNNNNGG 6 cut(s) 424, 636, 905, 1290, 1817, 2038
BsmAI GTCTC 3 cut(s) 45, 439, 898
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 4 cut(s) 476, 740, 1705, 2015
BsmI GAATGC 2 cut(s) 932, 1902
BsnI GGCC 4 cut(s) 948, 1401, 1667, 1835
Bso31I GGTCTC 1 cut(s) 45
BsoBI CYCGRG 1 cut(s) 1151
Bsp1286I GDGCHC 2 cut(s) 471, 2063
Bsp13I TCCGGA 1 cut(s) 1290
BspACI CCGC 8 cut(s) 123, 511, 1343, 1346, 1357, 1523, 1868, 1959
BspANI GGCC 4 cut(s) 948, 1401, 1667, 1835
BspCNI CTCAG 4 cut(s) 1096, 1700, 2065, 2109
BspDI ATCGAT 3 cut(s) 600, 1493, 1674
BspEI TCCGGA 1 cut(s) 1290
BspFNI CGCG 1 cut(s) 1878
BspLI GGNNCC 6 cut(s) 543, 756, 1579, 1809, 1936, 2062
BspMAI CTGCAG 2 cut(s) 787, 1600
BspPI GGATC 3 cut(s) 605, 1267, 1535
BspT107I GGYRCC 1 cut(s) 2060
BspTNI GGTCTC 1 cut(s) 45
BsrDI GCAATG 4 cut(s) 348, 976, 1863, 2133
BsrI ACTGG 5 cut(s) 1011, 1218, 1318, 1402, 1822
BssECI CCNNGG 7 cut(s) 100, 257, 899, 1150, 1151, 1182, 2037
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 4 cut(s) 100, 257, 899, 1182
Bst2UI CCWGG 1 cut(s) 2039
Bst4CI ACNGT 6 cut(s) 141, 188, 673, 827, 966, 1971
Bst6I CTCTTC 3 cut(s) 234, 1344, 1437
BstACI GRCGYC 1 cut(s) 489
BstAPI GCANNNNNTGC 1 cut(s) 190
BstC8I GCNNGC 4 cut(s) 1032, 1110, 1467, 1762
BstDEI CTNAG 6 cut(s) 235, 1104, 1687, 1697, 2052, 2096
BstENI CCTNNNNNAGG 1 cut(s) 903
BstF5I GGATG 5 cut(s) 375, 585, 1034, 1280, 1827
BstFNI CGCG 1 cut(s) 1878
BstHHI GCGC 2 cut(s) 1374, 1878
BstMAI GTCTC 3 cut(s) 45, 439, 898
BstMWI GCNNNNNNNGC 9 cut(s) 190, 1001, 1031, 1199, 1475, 1520, 1664, 1721, 1847
BstNI CCWGG 1 cut(s) 2039
BstNSI RCATGY 3 cut(s) 893, 1112, 1469
BstPAI GACNNNNGTC 1 cut(s) 916
BstSCI CCNGG 3 cut(s) 1150, 1151, 2037
BstSFI CTRYAG 3 cut(s) 354, 783, 1596
BstSLI GKGCMC 1 cut(s) 2063
BstUI CGCG 1 cut(s) 1878
BstV1I GCAGC 7 cut(s) 500, 991, 1224, 1582, 1610, 1702, 2101
BstV2I GAAGAC 4 cut(s) 170, 516, 1311, 1509
BstX2I RGATCY 2 cut(s) 578, 1259
BstYI RGATCY 2 cut(s) 578, 1259
Bsu15I ATCGAT 3 cut(s) 600, 1493, 1674
Bsu36I CCTNAGG 1 cut(s) 1687
BsuRI GGCC 4 cut(s) 948, 1401, 1667, 1835
BsuTUI ATCGAT 3 cut(s) 600, 1493, 1674
BtgZI GCGATG 2 cut(s) 402, 1529
BtrI CACGTC 1 cut(s) 1288
BtsCI GGATG 5 cut(s) 375, 585, 1034, 1280, 1827
BtsI GCAGTG 1 cut(s) 1025
BtsIMutI CAGTG 3 cut(s) 184, 1025, 1409
Cac8I GCNNGC 4 cut(s) 1032, 1110, 1467, 1762
CaiI CAGNNNCTG 1 cut(s) 1262
CfoI GCGC 2 cut(s) 1374, 1878
Cfr13I GGNCC 4 cut(s) 541, 754, 846, 1807
Cfr9I CCCGGG 1 cut(s) 1151
ClaI ATCGAT 3 cut(s) 600, 1493, 1674
CseI GACGC 1 cut(s) 478
Csp6I GTAC 2 cut(s) 505, 2004
CviQI GTAC 2 cut(s) 505, 2004
DdeI CTNAG 6 cut(s) 235, 1104, 1687, 1697, 2052, 2096
DrdI GACNNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 3 cut(s) 234, 1344, 1437
EarI CTCTTC 3 cut(s) 234, 1344, 1437
EciI GGCGGA 1 cut(s) 1361
Eco130I CCWWGG 4 cut(s) 100, 257, 899, 1182
Eco147I AGGCCT 1 cut(s) 1667
Eco31I GGTCTC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 1056
Eco47I GGWCC 4 cut(s) 541, 754, 846, 1807
Eco57I CTGAAG 5 cut(s) 164, 510, 543, 2000, 2089
Eco81I CCTNAGG 1 cut(s) 1687
Eco88I CYCGRG 1 cut(s) 1151
EcoNI CCTNNNNNAGG 1 cut(s) 903
EcoO109I RGGNCCY 1 cut(s) 846
EcoRI GAATTC 2 cut(s) 23, 449
EcoRII CCWGG 1 cut(s) 2037
EcoRV GATATC 1 cut(s) 1056
EcoT14I CCWWGG 4 cut(s) 100, 257, 899, 1182
ErhI CCWWGG 4 cut(s) 100, 257, 899, 1182
Esp3I CGTCTC 1 cut(s) 439
FaqI GGGAC 4 cut(s) 476, 740, 1705, 2015
FokI GGATG 5 cut(s) 382, 572, 1021, 1267, 1834
FspBI CTAG 9 cut(s) 18, 135, 900, 999, 1251, 1682, 1766, 1847, 1913
GlaI GCGC 2 cut(s) 1373, 1877
GsaI CCCAGC 1 cut(s) 1779
GsuI CTGGAG 3 cut(s) 1110, 1110, 1295
HaeIII GGCC 4 cut(s) 948, 1401, 1667, 1835
HapII CCGG 2 cut(s) 1152, 1291
HgaI GACGC 1 cut(s) 478
HhaI GCGC 2 cut(s) 1374, 1878
Hin1I GRCGYC 1 cut(s) 489
Hin6I GCGC 2 cut(s) 1372, 1876
HinP1I GCGC 2 cut(s) 1372, 1876
HincII GTYRAC 3 cut(s) 415, 1272, 1828
HindII GTYRAC 3 cut(s) 415, 1272, 1828
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 7 cut(s) 788, 989, 1136, 1384, 1693, 1928, 2110
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 2 cut(s) 1152, 1291
HphI GGTGA 1 cut(s) 948
Hpy166II GTNNAC 5 cut(s) 415, 911, 1272, 1828, 1860
Hpy188I TCNGA 8 cut(s) 229, 249, 318, 529, 733, 769, 1105, 1264
Hpy8I GTNNAC 5 cut(s) 415, 911, 1272, 1828, 1860
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 8 cut(s) 285, 510, 668, 1190, 1889, 1994, 2015, 2114
HpyCH4III ACNGT 6 cut(s) 141, 188, 673, 827, 966, 1971
HpyCH4IV ACGT 2 cut(s) 867, 1287
HpyF10VI GCNNNNNNNGC 9 cut(s) 190, 1001, 1031, 1199, 1475, 1520, 1664, 1721, 1847
HpyF3I CTNAG 6 cut(s) 235, 1104, 1687, 1697, 2052, 2096
HpySE526I ACGT 2 cut(s) 867, 1287
Hsp92I GRCGYC 1 cut(s) 489
HspAI GCGC 2 cut(s) 1372, 1876
Kpn2I TCCGGA 1 cut(s) 1290
KspAI GTTAAC 1 cut(s) 415
LmnI GCTCC 4 cut(s) 14, 984, 1577, 2024
Lsp1109I GCAGC 7 cut(s) 500, 991, 1224, 1582, 1610, 1702, 2101
LweI GCATC 5 cut(s) 491, 1043, 1289, 1702, 1798
MaeI CTAG 9 cut(s) 18, 135, 900, 999, 1251, 1682, 1766, 1847, 1913
MaeII ACGT 2 cut(s) 867, 1287
MaeIII GTNAC 3 cut(s) 1471, 1571, 2099
MfeI CAATTG 3 cut(s) 1536, 1884, 1976
MflI RGATCY 2 cut(s) 578, 1259
MhlI GDGCHC 2 cut(s) 471, 2063
MlyI GAGTC 1 cut(s) 1687
MmeI TCCRAC 1 cut(s) 841
MroI TCCGGA 1 cut(s) 1290
MroXI GAANNNNTTC 1 cut(s) 1074
MseI TTAA 8 cut(s) 62, 170, 312, 414, 1041, 1298, 1497, 1662
MslI CAYNNNNRTG 3 cut(s) 444, 1043, 1071
MspA1I CMGCKG 2 cut(s) 513, 1237
MspI CCGG 2 cut(s) 1152, 1291
MspR9I CCNGG 3 cut(s) 1152, 1153, 2039
MunI CAATTG 3 cut(s) 1536, 1884, 1976
Mva1269I GAATGC 2 cut(s) 932, 1902
MvaI CCWGG 1 cut(s) 2039
MvnI CGCG 1 cut(s) 1878
MwoI GCNNNNNNNGC 9 cut(s) 190, 1001, 1031, 1199, 1475, 1520, 1664, 1721, 1847
NciI CCSGG 2 cut(s) 1152, 1153
NlaIV GGNNCC 6 cut(s) 543, 756, 1579, 1809, 1936, 2062
NmuCI GTSAC 3 cut(s) 1471, 1571, 2099
NspI RCATGY 3 cut(s) 893, 1112, 1469
PaeI GCATGC 2 cut(s) 1112, 1469
PceI AGGCCT 1 cut(s) 1667
PciI ACATGT 1 cut(s) 889
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PctI GAATGC 2 cut(s) 932, 1902
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 6 cut(s) 788, 989, 1136, 1384, 1928, 2110
PflMI CCANNNNNTGG 1 cut(s) 424
PleI GAGTC 1 cut(s) 1687
PpsI GAGTC 1 cut(s) 1687
PpuMI RGGWCCY 1 cut(s) 846
PscI ACATGT 1 cut(s) 889
PshAI GACNNNNGTC 1 cut(s) 916
PshBI ATTAAT 2 cut(s) 1298, 1497
Psp5II RGGWCCY 1 cut(s) 846
Psp6I CCWGG 1 cut(s) 2037
PspFI CCCAGC 1 cut(s) 1775
PspGI CCWGG 1 cut(s) 2037
PspN4I GGNNCC 6 cut(s) 543, 756, 1579, 1809, 1936, 2062
PspPI GGNCC 4 cut(s) 541, 754, 846, 1807
PspPPI RGGWCCY 1 cut(s) 846
PstI CTGCAG 2 cut(s) 787, 1600
PstNI CAGNNNCTG 1 cut(s) 1262
PsuI RGATCY 2 cut(s) 578, 1259
PvuII CAGCTG 1 cut(s) 1237
RsaI GTAC 2 cut(s) 506, 2005
RsaNI GTAC 2 cut(s) 505, 2004
RseI CAYNNNNRTG 3 cut(s) 444, 1043, 1071
SaqAI TTAA 8 cut(s) 62, 170, 312, 414, 1041, 1298, 1497, 1662
Sau96I GGNCC 4 cut(s) 541, 754, 846, 1807
SchI GAGTC 1 cut(s) 1687
ScrFI CCNGG 3 cut(s) 1152, 1153, 2039
SduI GDGCHC 2 cut(s) 471, 2063
SfaNI GCATC 5 cut(s) 491, 1043, 1289, 1702, 1798
SfcI CTRYAG 3 cut(s) 354, 783, 1596
SinI GGWCC 4 cut(s) 541, 754, 846, 1807
SmaI CCCGGG 1 cut(s) 1153
SmiMI CAYNNNNRTG 3 cut(s) 444, 1043, 1071
SmlI CTYRAG 2 cut(s) 113, 163
SmoI CTYRAG 2 cut(s) 113, 163
SpeI ACTAGT 2 cut(s) 998, 1912
SphI GCATGC 2 cut(s) 1112, 1469
SseBI AGGCCT 1 cut(s) 1667
SsiI CCGC 8 cut(s) 123, 511, 1343, 1346, 1357, 1523, 1868, 1959
SspMI CTAG 9 cut(s) 18, 135, 900, 999, 1251, 1682, 1766, 1847, 1913
StuI AGGCCT 1 cut(s) 1667
StyD4I CCNGG 3 cut(s) 1150, 1151, 2037
StyI CCWWGG 4 cut(s) 100, 257, 899, 1182
TaaI ACNGT 6 cut(s) 141, 188, 673, 827, 966, 1971
TaiI ACGT 2 cut(s) 870, 1290
TaqII GACCGA 1 cut(s) 1638
TatI WGTACW 1 cut(s) 2003
TauI GCSGC 4 cut(s) 126, 1346, 1360, 1961
TfiI GAWTC 6 cut(s) 788, 989, 1136, 1384, 1928, 2110
Tru1I TTAA 8 cut(s) 62, 170, 312, 414, 1041, 1298, 1497, 1662
Tru9I TTAA 8 cut(s) 62, 170, 312, 414, 1041, 1298, 1497, 1662
TscAI CASTG 3 cut(s) 191, 1032, 1409
TseFI GTSAC 3 cut(s) 1471, 1571, 2099
TseI GCWGC 7 cut(s) 513, 1004, 1237, 1595, 1598, 1715, 2089
Tsp45I GTSAC 3 cut(s) 1471, 1571, 2099
TspGWI ACGGA 2 cut(s) 1394, 1590
TspMI CCCGGG 1 cut(s) 1151
TspRI CASTG 3 cut(s) 191, 1032, 1409
Van91I CCANNNNNTGG 1 cut(s) 424
VpaK11BI GGWCC 4 cut(s) 541, 754, 846, 1807
VspI ATTAAT 2 cut(s) 1298, 1497
XagI CCTNNNNNAGG 1 cut(s) 903
XapI RAATTY 6 cut(s) 23, 198, 449, 1486, 1588, 1852
XceI RCATGY 3 cut(s) 893, 1112, 1469
XmaI CCCGGG 1 cut(s) 1151
XmaJI CCTAGG 1 cut(s) 899
XmnI GAANNNNTTC 1 cut(s) 1074
XspI CTAG 9 cut(s) 18, 135, 900, 999, 1251, 1682, 1766, 1847, 1913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.