MD05G1196100.v1.1

50S ribosomal protein L10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016095
Physical Location & Seq
Reverse (-)
32348036 .. 32351089
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1196100.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGAAGCCACTCTCTTCAGCTTCCCTTGCTCCAAACCCCCATCACCCTCCCTAACCCAAACCCAACCCAAATCCCTCCTGGCCCGACCCTCCACCAACTCATTTCTCTCCCTCACCTCCCGCCCCACCCGCCACCACCACCACCACCACCACCGCCTCCCCACAATCCGCTCCGCCATCTCCCGCACCAAAAAGGAACAGACCGTCGAAACCATCAAGGAAAATCTCGAGAACTGCCACCTCATGGCCGGTATCCGCTACAAGGGCCTCACCGTAAAGCAATTCCAGGACCTCCGGAAAGTCCTGCCGGAGACCACCAAGCTCATTGTGGCGAAGAACACGCTGGTCTACAAGGCAATCGAGGGGACGCCGTGGGAGGCGCTCAAGCCGTGCATGACGGGGATGAACGCCTGGCTCTTCGTCCACAGCGAGGAAATCCCGCCGGCGATCAAGCCCTACAGGGATTTCCAGAAGGAGAAAAAGCTCGACAGCAACGACTTCACCGGGGCGGTTTTCGAAGGGAAATTTTACGGGCCTGGGGACTTTAAGCAGCTCGAGACAATGCCAACGAGGGCAGAGATTTACGCTAAGTTGCTTGGGACGTTGAATAGTCCGGCGTCGAGCTTGGTGGGGACTATTCAGGCGCCGGCGAGGGAGTTGGTCATGCTTTTGAAGGCTTATGTGCAGAAATTGGAGGAAGAAGGCGGCGGCGGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L10

Protein Analysis

240

Amino Acids

26.83

Weight (kDa)

9.44

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L10 PF00466 62 - 156 4.7e-20 Ribosomal protein L10
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13510
fragaria_vesca FvH4_2g21340
malus_domestica MD05G1196100.v1.1 MD10G1183400.v1.1
prunus_persica Prupe.8G222500_v2.0.a1
pyrus_communis pycom05g18310 pycom10g15840
rosa_chinensis RchiOBHm_Chr6g0287931
rosa_laevigata RLG00000012455
rosa_multiflora Rmu_sc0000042.1_g000003
rosa_roxburghii Rroxscaffold_7G00180660
rosa_rugosa Rorug06G0193500
rosa_samantha Rh6CG300800 Rh6DG301400
rosa_wichuraiana Rw6G026170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 643
AccB7I CCANNNNNTGG 1 cut(s) 244
AccBSI CCGCTC 1 cut(s) 171
AccI GTMKAC 1 cut(s) 348
AccIII TCCGGA 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 524
AcyI GRCGYC 3 cut(s) 368, 617, 644
AfiI CCNNNNNNNGG 3 cut(s) 244, 262, 430
AgsI TTSAA 2 cut(s) 607, 673
AjnI CCWGG 4 cut(s) 78, 285, 410, 535
AluBI AGCT 5 cut(s) 21, 322, 484, 553, 624
AluI AGCT 5 cut(s) 21, 322, 484, 553, 624
Alw26I GTCTC 2 cut(s) 305, 551
Ama87I CYCGRG 2 cut(s) 227, 554
Aor13HI TCCGGA 1 cut(s) 294
AoxI GGCC 4 cut(s) 81, 246, 265, 533
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 1 cut(s) 524
AspLEI GCGC 2 cut(s) 382, 646
AspS9I GGNCC 4 cut(s) 82, 265, 289, 533
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 4 cut(s) 36, 106, 262, 493
AsuII TTCGAA 1 cut(s) 516
AvaI CYCGRG 2 cut(s) 227, 554
AvaII GGWCC 1 cut(s) 289
BanI GGYRCC 1 cut(s) 643
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 49, 185, 221
BceAI ACGGC 2 cut(s) 355, 373
BciT130I CCWGG 4 cut(s) 80, 287, 412, 537
BciVI GTATCC 1 cut(s) 263
BcnI CCSGG 1 cut(s) 505
BcoDI GTCTC 2 cut(s) 305, 551
BfmI CTRYAG 1 cut(s) 457
BfoI RGCGCY 2 cut(s) 383, 647
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 3 cut(s) 551, 706, 709
BlsI GCNGC 3 cut(s) 552, 707, 710
Bme1390I CCNGG 5 cut(s) 80, 287, 412, 505, 537
Bme18I GGWCC 1 cut(s) 289
BmeT110I CYCGRG 2 cut(s) 227, 554
BmgT120I GGNCC 4 cut(s) 82, 265, 289, 533
BmiI GGNNCC 1 cut(s) 645
BmrFI CCNGG 5 cut(s) 80, 287, 412, 505, 537
Bpu14I TTCGAA 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 505
BsaHI GRCGYC 3 cut(s) 368, 617, 644
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 3 cut(s) 371, 504, 536
BsaWI WCCGGW 1 cut(s) 294
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 262, 430
Bse118I RCCGGY 3 cut(s) 248, 442, 646
BseAI TCCGGA 1 cut(s) 294
BseBI CCWGG 4 cut(s) 80, 287, 412, 537
BseDI CCNNGG 3 cut(s) 371, 504, 536
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 244, 262, 430
BseXI GCAGC 1 cut(s) 562
BsgI GTGCAG 1 cut(s) 704
BshFI GGCC 4 cut(s) 83, 248, 267, 535
BshNI GGYRCC 1 cut(s) 643
BsiHKCI CYCGRG 2 cut(s) 227, 554
BsiSI CCGG 7 cut(s) 249, 295, 308, 443, 504, 614, 647
BslFI GGGAC 4 cut(s) 379, 554, 613, 646
BslI CCNNNNNNNGG 3 cut(s) 244, 262, 430
BsmAI GTCTC 2 cut(s) 305, 551
BsmFI GGGAC 4 cut(s) 379, 554, 613, 646
BsnI GGCC 4 cut(s) 83, 248, 267, 535
Bso31I GGTCTC 1 cut(s) 305
BsoBI CYCGRG 2 cut(s) 227, 554
Bsp119I TTCGAA 1 cut(s) 516
Bsp13I TCCGGA 1 cut(s) 294
Bsp143I GATC 1 cut(s) 447
BspANI GGCC 4 cut(s) 83, 248, 267, 535
BspEI TCCGGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 645
BspQI GCTCTTC 1 cut(s) 422
BspT104I TTCGAA 1 cut(s) 516
BspT107I GGYRCC 1 cut(s) 643
BspTNI GGTCTC 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 171
BsrFI RCCGGY 3 cut(s) 248, 442, 646
BssAI RCCGGY 3 cut(s) 248, 442, 646
BssECI CCNNGG 3 cut(s) 371, 504, 536
BssMI GATC 1 cut(s) 447
BssNI GRCGYC 3 cut(s) 368, 617, 644
Bst2UI CCWGG 4 cut(s) 80, 287, 412, 537
Bst4CI ACNGT 2 cut(s) 205, 274
Bst6I CTCTTC 2 cut(s) 20, 422
BstACI GRCGYC 3 cut(s) 368, 617, 644
BstBI TTCGAA 1 cut(s) 516
BstC8I GCNNGC 3 cut(s) 444, 648, 713
BstDEI CTNAG 1 cut(s) 588
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 408
BstH2I RGCGCY 2 cut(s) 383, 647
BstHHI GCGC 2 cut(s) 382, 646
BstKTI GATC 1 cut(s) 450
BstMAI GTCTC 2 cut(s) 305, 551
BstMBI GATC 1 cut(s) 447
BstMWI GCNNNNNNNGC 3 cut(s) 27, 129, 264
BstNI CCWGG 4 cut(s) 80, 287, 412, 537
BstSCI CCNGG 5 cut(s) 78, 285, 410, 503, 535
BstSFI CTRYAG 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 562
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 4 cut(s) 83, 248, 267, 535
BtgI CCRYGG 1 cut(s) 371
BtsCI GGATG 1 cut(s) 408
Cac8I GCNNGC 3 cut(s) 444, 648, 713
CfoI GCGC 2 cut(s) 382, 646
Cfr10I RCCGGY 3 cut(s) 248, 442, 646
Cfr13I GGNCC 4 cut(s) 82, 265, 289, 533
CseI GACGC 2 cut(s) 376, 606
CviAII CATG 3 cut(s) 244, 394, 664
DdeI CTNAG 1 cut(s) 588
DinI GGCGCC 1 cut(s) 645
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 20, 422
EarI CTCTTC 2 cut(s) 20, 422
EciI GGCGGA 1 cut(s) 163
Eco31I GGTCTC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 289
Eco88I CYCGRG 2 cut(s) 227, 554
EcoO109I RGGNCCY 2 cut(s) 265, 289
EcoRII CCWGG 4 cut(s) 78, 285, 410, 535
EgeI GGCGCC 1 cut(s) 645
EheI GGCGCC 1 cut(s) 645
FaeI CATG 3 cut(s) 247, 397, 667
FaiI YATR 4 cut(s) 245, 395, 665, 681
FaqI GGGAC 4 cut(s) 379, 554, 613, 646
FatI CATG 3 cut(s) 243, 393, 663
FauI CCCGC 5 cut(s) 128, 137, 191, 447, 704
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 551, 706, 709
FokI GGATG 1 cut(s) 415
Fsp4HI GCNGC 3 cut(s) 551, 706, 709
GlaI GCGC 2 cut(s) 381, 645
GluI GCNGC 3 cut(s) 551, 706, 709
HaeII RGCGCY 2 cut(s) 383, 647
HaeIII GGCC 4 cut(s) 83, 248, 267, 535
HapII CCGG 7 cut(s) 249, 295, 308, 443, 504, 614, 647
HgaI GACGC 2 cut(s) 376, 606
HhaI GCGC 2 cut(s) 382, 646
Hin1I GRCGYC 3 cut(s) 368, 617, 644
Hin1II CATG 3 cut(s) 247, 397, 667
Hin6I GCGC 2 cut(s) 380, 644
HinP1I GCGC 2 cut(s) 380, 644
HpaII CCGG 7 cut(s) 249, 295, 308, 443, 504, 614, 647
HphI GGTGA 4 cut(s) 36, 106, 262, 493
Hpy166II GTNNAC 2 cut(s) 349, 424
Hpy188III TCNNGA 5 cut(s) 227, 229, 295, 469, 556
Hpy8I GTNNAC 2 cut(s) 349, 424
Hpy99I CGWCG 2 cut(s) 209, 622
HpyAV CCTTC 4 cut(s) 466, 512, 667, 695
HpyCH4III ACNGT 2 cut(s) 205, 274
HpyCH4IV ACGT 1 cut(s) 602
HpyCH4V TGCA 2 cut(s) 393, 685
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 129, 264
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 602
Hsp92I GRCGYC 3 cut(s) 368, 617, 644
Hsp92II CATG 3 cut(s) 247, 397, 667
HspAI GCGC 2 cut(s) 380, 644
KasI GGCGCC 1 cut(s) 643
Kpn2I TCCGGA 1 cut(s) 294
KroI GCCGGC 2 cut(s) 442, 646
KroNI GCCGGC 2 cut(s) 444, 648
Kzo9I GATC 1 cut(s) 447
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 2 cut(s) 35, 176
Lsp1109I GCAGC 1 cut(s) 562
MaeII ACGT 1 cut(s) 602
MalI GATC 1 cut(s) 449
MbiI CCGCTC 1 cut(s) 171
MboI GATC 1 cut(s) 447
MboII GAAGA 4 cut(s) 7, 346, 409, 710
MluCI AATT 3 cut(s) 281, 524, 689
Mly113I GGCGCC 1 cut(s) 644
MreI CGCCGGCG 2 cut(s) 442, 646
MroI TCCGGA 1 cut(s) 294
MroNI GCCGGC 2 cut(s) 442, 646
MseI TTAA 1 cut(s) 546
MspI CCGG 7 cut(s) 249, 295, 308, 443, 504, 614, 647
MspR9I CCNGG 5 cut(s) 80, 287, 412, 505, 537
MvaI CCWGG 4 cut(s) 80, 287, 412, 537
MwoI GCNNNNNNNGC 3 cut(s) 27, 129, 264
NaeI GCCGGC 2 cut(s) 444, 648
NarI GGCGCC 1 cut(s) 644
NciI CCSGG 1 cut(s) 505
NdeII GATC 1 cut(s) 447
NgoMIV GCCGGC 2 cut(s) 442, 646
NlaIII CATG 3 cut(s) 247, 397, 667
NlaIV GGNNCC 1 cut(s) 645
NspV TTCGAA 1 cut(s) 516
PaeR7I CTCGAG 2 cut(s) 227, 554
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 2 cut(s) 426, 492
PdiI GCCGGC 2 cut(s) 444, 648
PflMI CCANNNNNTGG 1 cut(s) 244
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 3 cut(s) 552, 707, 710
PluTI GGCGCC 1 cut(s) 647
PpuMI RGGWCCY 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 289
Psp6I CCWGG 4 cut(s) 78, 285, 410, 535
PspGI CCWGG 4 cut(s) 78, 285, 410, 535
PspN4I GGNNCC 1 cut(s) 645
PspPI GGNCC 4 cut(s) 82, 265, 289, 533
PspPPI RGGWCCY 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 422
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 3 cut(s) 551, 706, 709
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 4 cut(s) 82, 265, 289, 533
ScrFI CCNGG 5 cut(s) 80, 287, 412, 505, 537
SetI ASST 9 cut(s) 23, 119, 243, 294, 324, 486, 555, 605, 626
SfcI CTRYAG 1 cut(s) 457
SfoI GGCGCC 1 cut(s) 645
Sfr274I CTCGAG 2 cut(s) 227, 554
SfuI TTCGAA 1 cut(s) 516
SgrAI CRCCGGYG 2 cut(s) 442, 646
SinI GGWCC 1 cut(s) 289
SlaI CTCGAG 2 cut(s) 227, 554
SmlI CTYRAG 3 cut(s) 227, 383, 554
SmoI CTYRAG 3 cut(s) 227, 383, 554
Sse9I AATT 3 cut(s) 281, 524, 689
SspDI GGCGCC 1 cut(s) 643
StyD4I CCNGG 5 cut(s) 78, 285, 410, 503, 535
TaaI ACNGT 2 cut(s) 205, 274
TaiI ACGT 1 cut(s) 605
TaqI TCGA 7 cut(s) 207, 228, 360, 486, 516, 555, 620
TasI AATT 3 cut(s) 281, 524, 689
TauI GCSGC 2 cut(s) 708, 711
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TseI GCWGC 1 cut(s) 550
TspDTI ATGAA 1 cut(s) 419
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 289
XapI RAATTY 1 cut(s) 524
XcmI CCANNNNNNNNNTGG 2 cut(s) 76, 325
XhoI CTCGAG 2 cut(s) 227, 554
XmiI GTMKAC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.