pycom05g18310

50S ribosomal protein L10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016095
Physical Location & Seq
Reverse (-)
21298026 .. 21298910
885 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18310.2

Sequence Viewer

Length: 822 bp
ATGAAAATTGGGAATTGGAATTTTCCCACCCTACCCCTTCTCAAAAATTGGACGGATAAGATAGAGGAAAAGCCCCAAAACGCACTCTCGCTTCTCAATTCATATTCTGCGACCATGGAAGCCACTCTCTTCAGCTTCCCTTACTCCAAACCCCCATCACCCTCCCTAACCCAAACCCACCCCGAATCCCTCCTGACCCGACCCTCCACCAACCCATTTCTCTCCCTCACCTCCCGCCCCGCCCGCCGCCACCACCGCCTCCCCACCATCCGCTCCGCCATCTCCCGCACCAAAAAGGAACAGACCGTCGAAACCATCAAGGAAAATCTCGAAAACTGCCACCTCATGGCCGGTATCCGCTACAAGGGCCTCACCGTAAAGCAATTCCAGGACCTCCGGAAGGTCCTGCCGGAGACCACCAAGCTCATTGTGGCGAAGAACACGCTGGTCTACAAGGCAATCGAGGGGACGCCGTGGGAGGTGCTCAAGCCGTGCATGACGGGGATGAACGCCTGGCTCTTCGTCCACAGCGAGGAAATCCCGCCGGCGATCAAGCCCTACAGGGATTTCCAGAAGGAGAAAAAGCTCGACAGCAACGACTTCACCGGGGCGGTTTTCGAAGGGAAATTTTACGGGCCTGGGGACTTTAAGCAGCTCGAGACAATGCCAACGAGGGCAGAGATTTACGCTAAGTTGCTTGGGACGTTGAATAGTCCGGCCTCGAGCTTGGTGGGGACGATTCAGGCGCCGGCGAGGGAGTTGATCATGCTTTTGAAGGCTTATGTGCAGAAATTGGAGGAAGAAGGCGGCGGCGGTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L10

Protein Analysis

274

Amino Acids

30.78

Weight (kDa)

9.41

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13510
fragaria_vesca FvH4_2g21340
malus_domestica MD05G1196100.v1.1 MD10G1183400.v1.1
prunus_persica Prupe.8G222500_v2.0.a1
pyrus_communis pycom05g18310 pycom10g15840
rosa_chinensis RchiOBHm_Chr6g0287931
rosa_laevigata RLG00000012455
rosa_multiflora Rmu_sc0000042.1_g000003
rosa_roxburghii Rroxscaffold_7G00180660
rosa_rugosa Rorug06G0193500
rosa_samantha Rh6CG300800 Rh6DG301400
rosa_wichuraiana Rw6G026170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 745
AccB7I CCANNNNNTGG 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 2 cut(s) 19, 626
AcuI CTGAAG 1 cut(s) 115
AcyI GRCGYC 2 cut(s) 470, 746
AfiI CCNNNNNNNGG 4 cut(s) 346, 364, 400, 532
AgsI TTSAA 2 cut(s) 709, 775
AjnI CCWGG 3 cut(s) 387, 512, 637
AluBI AGCT 5 cut(s) 135, 424, 586, 655, 726
AluI AGCT 5 cut(s) 135, 424, 586, 655, 726
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 2 cut(s) 407, 653
Ama87I CYCGRG 2 cut(s) 656, 721
Aor13HI TCCGGA 1 cut(s) 396
AoxI GGCC 4 cut(s) 348, 367, 635, 717
ApeKI GCWGC 1 cut(s) 652
ApoI RAATTY 2 cut(s) 19, 626
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 4 cut(s) 367, 391, 403, 635
AsuC2I CCSGG 1 cut(s) 607
AsuHPI GGTGA 4 cut(s) 150, 220, 364, 595
AsuII TTCGAA 1 cut(s) 618
AvaI CYCGRG 2 cut(s) 656, 721
AvaII GGWCC 2 cut(s) 391, 403
BanI GGYRCC 1 cut(s) 745
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 664
BccI CCATC 4 cut(s) 163, 275, 287, 323
BceAI ACGGC 2 cut(s) 457, 475
BciT130I CCWGG 3 cut(s) 389, 514, 639
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 762
BcnI CCSGG 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 407, 653
BfmI CTRYAG 1 cut(s) 559
BfoI RGCGCY 1 cut(s) 749
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 4 cut(s) 247, 653, 808, 811
BlsI GCNGC 4 cut(s) 248, 654, 809, 812
Bme1390I CCNGG 4 cut(s) 389, 514, 607, 639
Bme18I GGWCC 2 cut(s) 391, 403
BmeT110I CYCGRG 2 cut(s) 656, 721
BmgT120I GGNCC 4 cut(s) 367, 391, 403, 635
BmiI GGNNCC 1 cut(s) 747
BmrFI CCNGG 4 cut(s) 389, 514, 607, 639
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 607
BsaHI GRCGYC 2 cut(s) 470, 746
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 4 cut(s) 114, 473, 606, 638
BsaWI WCCGGW 1 cut(s) 396
Bsc4I CCNNNNNNNGG 4 cut(s) 346, 364, 400, 532
Bse118I RCCGGY 3 cut(s) 350, 544, 748
BseAI TCCGGA 1 cut(s) 396
BseBI CCWGG 3 cut(s) 389, 514, 639
BseDI CCNNGG 4 cut(s) 114, 473, 606, 638
BseGI GGATG 2 cut(s) 267, 510
BseLI CCNNNNNNNGG 4 cut(s) 346, 364, 400, 532
BseXI GCAGC 1 cut(s) 664
BsgI GTGCAG 1 cut(s) 806
BshFI GGCC 4 cut(s) 350, 369, 637, 719
BshNI GGYRCC 1 cut(s) 745
BsiHKAI GWGCWC 1 cut(s) 486
BsiHKCI CYCGRG 2 cut(s) 656, 721
BsiSI CCGG 7 cut(s) 351, 397, 410, 545, 606, 716, 749
BslFI GGGAC 4 cut(s) 481, 656, 715, 748
BslI CCNNNNNNNGG 4 cut(s) 346, 364, 400, 532
BsmAI GTCTC 2 cut(s) 407, 653
BsmFI GGGAC 4 cut(s) 481, 656, 715, 748
BsnI GGCC 4 cut(s) 350, 369, 637, 719
Bso31I GGTCTC 1 cut(s) 407
BsoBI CYCGRG 2 cut(s) 656, 721
Bsp119I TTCGAA 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 486
Bsp13I TCCGGA 1 cut(s) 396
Bsp143I GATC 2 cut(s) 549, 762
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 4 cut(s) 350, 369, 637, 719
BspEI TCCGGA 1 cut(s) 396
BspLI GGNNCC 1 cut(s) 747
BspQI GCTCTTC 1 cut(s) 524
BspT104I TTCGAA 1 cut(s) 618
BspT107I GGYRCC 1 cut(s) 745
BspTNI GGTCTC 1 cut(s) 407
BsrBI CCGCTC 1 cut(s) 273
BsrFI RCCGGY 3 cut(s) 350, 544, 748
BssAI RCCGGY 3 cut(s) 350, 544, 748
BssECI CCNNGG 4 cut(s) 114, 473, 606, 638
BssMI GATC 2 cut(s) 549, 762
BssNI GRCGYC 2 cut(s) 470, 746
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 3 cut(s) 389, 514, 639
Bst4CI ACNGT 2 cut(s) 307, 376
Bst6I CTCTTC 2 cut(s) 134, 524
BstACI GRCGYC 2 cut(s) 470, 746
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 244, 546, 750
BstDEI CTNAG 1 cut(s) 690
BstDSI CCRYGG 2 cut(s) 114, 473
BstENI CCTNNNNNAGG 1 cut(s) 398
BstF5I GGATG 2 cut(s) 267, 510
BstH2I RGCGCY 1 cut(s) 749
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 2 cut(s) 552, 765
BstMAI GTCTC 2 cut(s) 407, 653
BstMBI GATC 2 cut(s) 549, 762
BstMWI GCNNNNNNNGC 3 cut(s) 243, 255, 366
BstNI CCWGG 3 cut(s) 389, 514, 639
BstSCI CCNGG 4 cut(s) 387, 512, 605, 637
BstSFI CTRYAG 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 664
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 4 cut(s) 350, 369, 637, 719
BtgI CCRYGG 2 cut(s) 114, 473
BtsCI GGATG 2 cut(s) 267, 510
Cac8I GCNNGC 3 cut(s) 244, 546, 750
CfoI GCGC 1 cut(s) 748
Cfr10I RCCGGY 3 cut(s) 350, 544, 748
Cfr13I GGNCC 4 cut(s) 367, 391, 403, 635
CseI GACGC 1 cut(s) 478
CviAII CATG 4 cut(s) 115, 346, 496, 766
DdeI CTNAG 1 cut(s) 690
DinI GGCGCC 1 cut(s) 747
DpnI GATC 2 cut(s) 551, 764
DpnII GATC 2 cut(s) 549, 762
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 134, 524
EarI CTCTTC 2 cut(s) 134, 524
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 407
Eco47I GGWCC 2 cut(s) 391, 403
Eco57I CTGAAG 1 cut(s) 115
Eco88I CYCGRG 2 cut(s) 656, 721
EcoNI CCTNNNNNAGG 1 cut(s) 398
EcoO109I RGGNCCY 3 cut(s) 367, 391, 403
EcoRII CCWGG 3 cut(s) 387, 512, 637
EcoT14I CCWWGG 1 cut(s) 114
EgeI GGCGCC 1 cut(s) 747
EheI GGCGCC 1 cut(s) 747
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 4 cut(s) 118, 349, 499, 769
FaiI YATR 6 cut(s) 103, 116, 347, 497, 767, 783
FaqI GGGAC 4 cut(s) 481, 656, 715, 748
FatI CATG 4 cut(s) 114, 345, 495, 765
FauI CCCGC 5 cut(s) 242, 247, 251, 293, 549
FbaI TGATCA 1 cut(s) 762
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 4 cut(s) 247, 653, 808, 811
FokI GGATG 2 cut(s) 254, 517
Fsp4HI GCNGC 4 cut(s) 247, 653, 808, 811
GlaI GCGC 1 cut(s) 747
GluI GCNGC 4 cut(s) 247, 653, 808, 811
HaeII RGCGCY 1 cut(s) 749
HaeIII GGCC 4 cut(s) 350, 369, 637, 719
HapII CCGG 7 cut(s) 351, 397, 410, 545, 606, 716, 749
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 748
Hin1I GRCGYC 2 cut(s) 470, 746
Hin1II CATG 4 cut(s) 118, 349, 499, 769
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HinfI GANTC 2 cut(s) 185, 739
HpaII CCGG 7 cut(s) 351, 397, 410, 545, 606, 716, 749
HphI GGTGA 4 cut(s) 150, 220, 364, 595
Hpy166II GTNNAC 2 cut(s) 451, 526
Hpy188III TCNNGA 5 cut(s) 193, 329, 397, 571, 658
Hpy8I GTNNAC 2 cut(s) 451, 526
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 6 cut(s) 47, 394, 568, 614, 769, 797
HpyCH4III ACNGT 2 cut(s) 307, 376
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 2 cut(s) 495, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 255, 366
HpyF3I CTNAG 1 cut(s) 690
HpySE526I ACGT 1 cut(s) 704
Hsp92I GRCGYC 2 cut(s) 470, 746
Hsp92II CATG 4 cut(s) 118, 349, 499, 769
HspAI GCGC 1 cut(s) 746
KasI GGCGCC 1 cut(s) 745
Kpn2I TCCGGA 1 cut(s) 396
KroI GCCGGC 2 cut(s) 544, 748
KroNI GCCGGC 2 cut(s) 546, 750
Ksp22I TGATCA 1 cut(s) 762
Kzo9I GATC 2 cut(s) 549, 762
LguI GCTCTTC 1 cut(s) 524
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 1 cut(s) 664
MaeII ACGT 1 cut(s) 704
MalI GATC 2 cut(s) 551, 764
MbiI CCGCTC 1 cut(s) 273
MboI GATC 2 cut(s) 549, 762
MboII GAAGA 4 cut(s) 121, 448, 511, 812
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 8 cut(s) 6, 13, 19, 46, 97, 383, 626, 791
Mly113I GGCGCC 1 cut(s) 746
MreI CGCCGGCG 2 cut(s) 544, 748
MroI TCCGGA 1 cut(s) 396
MroNI GCCGGC 2 cut(s) 544, 748
MseI TTAA 1 cut(s) 648
MspI CCGG 7 cut(s) 351, 397, 410, 545, 606, 716, 749
MspR9I CCNGG 4 cut(s) 389, 514, 607, 639
MvaI CCWGG 3 cut(s) 389, 514, 639
MwoI GCNNNNNNNGC 3 cut(s) 243, 255, 366
NaeI GCCGGC 2 cut(s) 546, 750
NarI GGCGCC 1 cut(s) 746
NciI CCSGG 1 cut(s) 607
NcoI CCATGG 1 cut(s) 114
NdeII GATC 2 cut(s) 549, 762
NgoMIV GCCGGC 2 cut(s) 544, 748
NlaIII CATG 4 cut(s) 118, 349, 499, 769
NlaIV GGNNCC 1 cut(s) 747
NspV TTCGAA 1 cut(s) 618
PaeR7I CTCGAG 2 cut(s) 656, 721
PciSI GCTCTTC 1 cut(s) 524
PcsI WCGNNNNNNNCGW 2 cut(s) 528, 594
PdiI GCCGGC 2 cut(s) 546, 750
PfeI GAWTC 2 cut(s) 185, 739
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 4 cut(s) 248, 654, 809, 812
PluTI GGCGCC 1 cut(s) 749
PpuMI RGGWCCY 2 cut(s) 391, 403
Psp5II RGGWCCY 2 cut(s) 391, 403
Psp6I CCWGG 3 cut(s) 387, 512, 637
PspGI CCWGG 3 cut(s) 387, 512, 637
PspN4I GGNNCC 1 cut(s) 747
PspPI GGNCC 4 cut(s) 367, 391, 403, 635
PspPPI RGGWCCY 2 cut(s) 391, 403
PspXI VCTCGAGB 1 cut(s) 721
SapI GCTCTTC 1 cut(s) 524
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 4 cut(s) 247, 653, 808, 811
Sau3AI GATC 2 cut(s) 549, 762
Sau96I GGNCC 4 cut(s) 367, 391, 403, 635
ScrFI CCNGG 4 cut(s) 389, 514, 607, 639
SduI GDGCHC 1 cut(s) 486
SfcI CTRYAG 1 cut(s) 559
SfoI GGCGCC 1 cut(s) 747
Sfr274I CTCGAG 2 cut(s) 656, 721
SfuI TTCGAA 1 cut(s) 618
SgrAI CRCCGGYG 2 cut(s) 544, 748
SinI GGWCC 2 cut(s) 391, 403
SlaI CTCGAG 2 cut(s) 656, 721
SmlI CTYRAG 3 cut(s) 485, 656, 721
SmoI CTYRAG 3 cut(s) 485, 656, 721
Sse9I AATT 8 cut(s) 6, 13, 19, 46, 97, 383, 626, 791
SspDI GGCGCC 1 cut(s) 745
StyD4I CCNGG 4 cut(s) 387, 512, 605, 637
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 2 cut(s) 307, 376
TaiI ACGT 1 cut(s) 707
TaqI TCGA 7 cut(s) 309, 330, 462, 588, 618, 657, 722
TasI AATT 8 cut(s) 6, 13, 19, 46, 97, 383, 626, 791
TauI GCSGC 3 cut(s) 249, 810, 813
TfiI GAWTC 2 cut(s) 185, 739
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TseI GCWGC 1 cut(s) 652
TspDTI ATGAA 3 cut(s) 17, 90, 521
TspGWI ACGGA 1 cut(s) 68
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 391, 403
XagI CCTNNNNNAGG 1 cut(s) 398
XapI RAATTY 2 cut(s) 19, 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XhoI CTCGAG 2 cut(s) 656, 721
XmiI GTMKAC 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.