Prupe.8G222500_v2.0.a1

50S ribosomal protein L10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0016095
Physical Location & Seq
Reverse (-)
20156427 .. 20157178
752 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G222500.1

Sequence Viewer

Length: 705 bp
ATGGAAGCCACTCTCTTCAGCTTCCCTTCCTCCAAACCCCCATCACCTTCCCTAACCCAAACCCAGTCCAAAACCCTTCTGACCCGACCCACCAACCCATTTCTCTCCCTCACCCCCCGACCCACCCGCCACCACCACCGCCTGCCCACAATCCGATCTGCCATCTCGCGCACCAAGAAAGAACAGACCGTCGAAACCATCAAAGAGAATCTCGAGAACTGCCATCTCTTGGCCGGCATCAAATACAAAGGCCTCACCGTGAAGCAATTTCAAGACCTCCGCAAAGTCCTACCCGAGACCACAAAGCTCATTGTGGCCAAGAACACTCTGGTCTACAAAGCCATAGAGGGAACTCCATGGGAGGCTCTCAAGCCCTGCATGACCGGCATGAACGCTTGGCTCTTTGTCCACAGCGAAGAAATCCCACCTGCCATTAAGCCTTACAGGGACTTTCAGAAGGAGAAGAAGCTCGACAGCAATGACTTCACCGGTGCTGTGTTTGAAGGCAAGTTTTATGCGCCTGGGGATTTTAAGCAGCTTGAGACAATGCCAACGAGGGCTGAGATTTATGCTAAGTTGCTCGGGACGTTGAATAGTCCGGCGTCGAGCTTGGTCGGGACAATACAGGCGCCGGCGAGGGAGTTGGTCATGCTTTTGAAGGCTTATGTGCAGAAATTGGAGGAGGAGAGCGGTGGTGGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L10

Protein Analysis

235

Amino Acids

26.23

Weight (kDa)

9.48

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13510
fragaria_vesca FvH4_2g21340
malus_domestica MD05G1196100.v1.1 MD10G1183400.v1.1
prunus_persica Prupe.8G222500_v2.0.a1
pyrus_communis pycom05g18310 pycom10g15840
rosa_chinensis RchiOBHm_Chr6g0287931
rosa_laevigata RLG00000012455
rosa_multiflora Rmu_sc0000042.1_g000003
rosa_roxburghii Rroxscaffold_7G00180660
rosa_rugosa Rorug06G0193500
rosa_samantha Rh6CG300800 Rh6DG301400
rosa_wichuraiana Rw6G026170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 1 cut(s) 628
AccB7I CCANNNNNTGG 1 cut(s) 229
AccBSI CCGCTC 1 cut(s) 690
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 169
AciI CCGC 4 cut(s) 127, 139, 280, 690
AcoI YGGCCR 2 cut(s) 231, 315
AcyI GRCGYC 2 cut(s) 602, 629
AfiI CCNNNNNNNGG 1 cut(s) 229
AgeI ACCGGT 1 cut(s) 488
AgsI TTSAA 4 cut(s) 272, 503, 592, 658
AjnI CCWGG 1 cut(s) 520
AluBI AGCT 5 cut(s) 21, 307, 469, 538, 609
AluI AGCT 5 cut(s) 21, 307, 469, 538, 609
Alw26I GTCTC 2 cut(s) 290, 536
Ama87I CYCGRG 3 cut(s) 212, 293, 581
AoxI GGCC 3 cut(s) 231, 250, 315
ApeKI GCWGC 1 cut(s) 535
AsiGI ACCGGT 1 cut(s) 488
AspLEI GCGC 3 cut(s) 171, 520, 631
AsuHPI GGTGA 4 cut(s) 36, 103, 247, 478
AvaI CYCGRG 3 cut(s) 212, 293, 581
BalI TGGCCA 1 cut(s) 317
BanI GGYRCC 1 cut(s) 628
BbvI GCAGC 1 cut(s) 547
BccI CCATC 4 cut(s) 49, 170, 206, 231
BciT130I CCWGG 1 cut(s) 522
BcoDI GTCTC 2 cut(s) 290, 536
BfoI RGCGCY 1 cut(s) 632
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 1 cut(s) 536
BlsI GCNGC 1 cut(s) 537
Bme1390I CCNGG 1 cut(s) 522
BmeT110I CYCGRG 3 cut(s) 212, 293, 581
BmiI GGNNCC 1 cut(s) 630
BmrFI CCNGG 1 cut(s) 522
BmrI ACTGGG 1 cut(s) 58
BmsI GCATC 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 58
BpuEI CTTGAG 2 cut(s) 353, 560
BsaHI GRCGYC 2 cut(s) 602, 629
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 356, 521
BsaWI WCCGGW 1 cut(s) 488
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse118I RCCGGY 4 cut(s) 233, 383, 488, 631
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 484
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 356, 521
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 64
BseRI GAGGAG 2 cut(s) 695, 698
BseXI GCAGC 1 cut(s) 547
BsgI GTGCAG 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 3 cut(s) 233, 252, 317
BshNI GGYRCC 1 cut(s) 628
BshTI ACCGGT 1 cut(s) 488
BsiHKCI CYCGRG 3 cut(s) 212, 293, 581
BsiSI CCGG 5 cut(s) 234, 384, 489, 599, 632
BslFI GGGAC 3 cut(s) 461, 598, 631
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 290, 536
BsmFI GGGAC 3 cut(s) 461, 598, 631
BsnI GGCC 3 cut(s) 233, 252, 317
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 3 cut(s) 212, 293, 581
Bsp143I GATC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 356
BspACI CCGC 4 cut(s) 127, 139, 280, 690
BspANI GGCC 3 cut(s) 233, 252, 317
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 630
BspMI ACCTGC 1 cut(s) 436
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 484
BsrFI RCCGGY 4 cut(s) 233, 383, 488, 631
BsrI ACTGG 1 cut(s) 64
BssAI RCCGGY 4 cut(s) 233, 383, 488, 631
BssECI CCNNGG 2 cut(s) 356, 521
BssMI GATC 1 cut(s) 155
BssNI GRCGYC 2 cut(s) 602, 629
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 2 cut(s) 190, 259
Bst6I CTCTTC 1 cut(s) 20
BstACI GRCGYC 2 cut(s) 602, 629
BstC8I GCNNGC 3 cut(s) 143, 235, 633
BstDEI CTNAG 2 cut(s) 561, 573
BstDSI CCRYGG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 3 cut(s) 171, 520, 631
BstKTI GATC 1 cut(s) 158
BstMAI GTCTC 2 cut(s) 290, 536
BstMBI GATC 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstNI CCWGG 1 cut(s) 522
BstSCI CCNGG 1 cut(s) 520
BstUI CGCG 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 547
BsuRI GGCC 3 cut(s) 233, 252, 317
BtgI CCRYGG 1 cut(s) 356
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 3 cut(s) 143, 235, 633
CfoI GCGC 3 cut(s) 171, 520, 631
Cfr10I RCCGGY 4 cut(s) 233, 383, 488, 631
CseI GACGC 1 cut(s) 591
CspAI ACCGGT 1 cut(s) 488
CviAII CATG 4 cut(s) 357, 379, 388, 649
DdeI CTNAG 2 cut(s) 561, 573
DinI GGCGCC 1 cut(s) 630
DpnI GATC 1 cut(s) 157
DpnII GATC 1 cut(s) 155
EaeI YGGCCR 2 cut(s) 231, 315
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 252
Eco31I GGTCTC 1 cut(s) 290
Eco88I CYCGRG 3 cut(s) 212, 293, 581
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 356
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 4 cut(s) 360, 382, 391, 652
FaiI YATR 8 cut(s) 344, 358, 380, 389, 516, 570, 650, 666
FaqI GGGAC 3 cut(s) 461, 598, 631
FatI CATG 4 cut(s) 356, 378, 387, 648
FauI CCCGC 1 cut(s) 134
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 536
Fsp4HI GCNGC 1 cut(s) 536
GlaI GCGC 3 cut(s) 170, 519, 630
GluI GCNGC 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 3 cut(s) 233, 252, 317
HapII CCGG 5 cut(s) 234, 384, 489, 599, 632
HgaI GACGC 1 cut(s) 591
HhaI GCGC 3 cut(s) 171, 520, 631
Hin1I GRCGYC 2 cut(s) 602, 629
Hin1II CATG 4 cut(s) 360, 382, 391, 652
Hin6I GCGC 3 cut(s) 169, 518, 629
HinP1I GCGC 3 cut(s) 169, 518, 629
HinfI GANTC 1 cut(s) 208
HpaII CCGG 5 cut(s) 234, 384, 489, 599, 632
HphI GGTGA 4 cut(s) 36, 103, 247, 478
Hpy166II GTNNAC 2 cut(s) 334, 409
Hpy188I TCNGA 3 cut(s) 81, 155, 456
Hpy188III TCNNGA 5 cut(s) 212, 214, 272, 583, 616
Hpy8I GTNNAC 2 cut(s) 334, 409
Hpy99I CGWCG 2 cut(s) 194, 607
HpyAV CCTTC 6 cut(s) 36, 57, 86, 451, 497, 652
HpyCH4III ACNGT 2 cut(s) 190, 259
HpyCH4IV ACGT 1 cut(s) 587
HpyCH4V TGCA 2 cut(s) 378, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpyF3I CTNAG 2 cut(s) 561, 573
HpySE526I ACGT 1 cut(s) 587
Hsp92I GRCGYC 2 cut(s) 602, 629
Hsp92II CATG 4 cut(s) 360, 382, 391, 652
HspAI GCGC 3 cut(s) 169, 518, 629
KasI GGCGCC 1 cut(s) 628
KroI GCCGGC 2 cut(s) 233, 631
KroNI GCCGGC 2 cut(s) 235, 633
Kzo9I GATC 1 cut(s) 155
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 1 cut(s) 246
MaeII ACGT 1 cut(s) 587
MalI GATC 1 cut(s) 157
MbiI CCGCTC 1 cut(s) 690
MboI GATC 1 cut(s) 155
MboII GAAGA 3 cut(s) 7, 428, 475
MlsI TGGCCA 1 cut(s) 317
MluCI AATT 2 cut(s) 266, 674
MluNI TGGCCA 1 cut(s) 317
Mly113I GGCGCC 1 cut(s) 629
Mox20I TGGCCA 1 cut(s) 317
MreI CGCCGGCG 1 cut(s) 631
MroNI GCCGGC 2 cut(s) 233, 631
MscI TGGCCA 1 cut(s) 317
MseI TTAA 2 cut(s) 435, 531
Msp20I TGGCCA 1 cut(s) 317
MspI CCGG 5 cut(s) 234, 384, 489, 599, 632
MspR9I CCNGG 1 cut(s) 522
MvaI CCWGG 1 cut(s) 522
MvnI CGCG 1 cut(s) 169
MwoI GCNNNNNNNGC 1 cut(s) 384
NaeI GCCGGC 2 cut(s) 235, 633
NarI GGCGCC 1 cut(s) 629
NcoI CCATGG 1 cut(s) 356
NdeII GATC 1 cut(s) 155
NgoMIV GCCGGC 2 cut(s) 233, 631
NlaIII CATG 4 cut(s) 360, 382, 391, 652
NlaIV GGNNCC 1 cut(s) 630
PaeR7I CTCGAG 1 cut(s) 212
PaqCI CACCTGC 1 cut(s) 436
PceI AGGCCT 1 cut(s) 252
PdiI GCCGGC 2 cut(s) 235, 633
PfeI GAWTC 1 cut(s) 208
PflMI CCANNNNNTGG 1 cut(s) 229
PinAI ACCGGT 1 cut(s) 488
PkrI GCNGC 1 cut(s) 537
PluTI GGCGCC 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 520
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 435, 531
SatI GCNGC 1 cut(s) 536
Sau3AI GATC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 522
SetI ASST 9 cut(s) 23, 49, 279, 309, 430, 471, 540, 590, 611
SfaNI GCATC 1 cut(s) 246
SfoI GGCGCC 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 212
SgrAI CRCCGGYG 2 cut(s) 488, 631
SlaI CTCGAG 1 cut(s) 212
SmlI CTYRAG 3 cut(s) 212, 368, 539
SmoI CTYRAG 3 cut(s) 212, 368, 539
Sse9I AATT 2 cut(s) 266, 674
SseBI AGGCCT 1 cut(s) 252
SsiI CCGC 4 cut(s) 127, 139, 280, 690
SspDI GGCGCC 1 cut(s) 628
StuI AGGCCT 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 2 cut(s) 190, 259
TaiI ACGT 1 cut(s) 590
TaqI TCGA 4 cut(s) 192, 213, 471, 605
TasI AATT 2 cut(s) 266, 674
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 2 cut(s) 435, 531
Tru9I TTAA 2 cut(s) 435, 531
TseI GCWGC 1 cut(s) 535
TspDTI ATGAA 1 cut(s) 404
Van91I CCANNNNNTGG 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.