MD05G1197700.v1.1

UPF0420 protein C16orf58 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009210
Physical Location & Seq
Reverse (-)
32468105 .. 32471265
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1197700.v1.1.491

Sequence Viewer

Length: 1314 bp
ATGAACTTGCTGGAGAAGATAAAGATGCAGAAGAAGGAGCCAGATAAAGCTCCAGCCCCTCCTGAGGTGCCGGCGTACTGGATTGAAACTTCCGAGTCGGTCTCCTACCGTTGCGAGTTTGACCCCACGGGTCAACTTTCTGTGAAGATTCTTGATGATTCGAGGCCAGTATTCCGTAAGGTCGGTGACTCGTTTGTCAATAAATTTTTTCCCTCGGGATATCCATATAGATACACACAATTCCGGGCACTGCAGCACTTAACCAGTGCAGCCCTTTCAGTGCTTTCAACTCAGTCACTCCTATTTGCTGCAGGCTTGCGACCAACCCCTGCACAGGCAACTGTTGTAAGCTGGGTTCTAAAGGATGGGATGCAGCACATGGGAAAGATTATATGTAGCAATCTGGGCGCAAGAATGGATTCTGAGCCTAAACGTTGGAGAATTTTGGCTGACGTGCTCTATGACTTTGGCACTGGCATGGAAGTTCTTTCTCCTTTGTGTCCGCAGCTTTTTCTTCAAGTCGCAGGTTTAGGCAATTTTGCAAAGGGGATGGCAGTTGTTGCAGCGAGAGCAACAAGATTACCAATTTATTCGGCCTTTGCAAAAGAGGGCAATCTTAGTGATCTGTTTGCCAAAGGGGAGGCAATCTCAACTGTGTTCAATGTCATTGGAATAGGAGCAGGGATCCAATTAGCATCTACTGTCTGCTTGTCTATGCAAGGAAAGATGGTTGTTGGGCCTCTTCTTTCAATGGTGCATGTATACTGTGTTATTGAAGAAATGCGGGCAACTCCTATCAACACATTGAATCCACAGAGGACTGCAATGATTGTGGCTGATTTTCTTAAGACAGGAAAAGTATCAAGCCCGGCTGACCTGAGGTATCGGGAAGACCTCCTATTCCCGGGGCGACTCATAAAGGATGCCGGAAATGTAAGAGTGGGAAGGTCTTTCCACGAGGTCATTAAGCCTTCAAAACTTGGTGAATTGAAAGAAATATTCCCCGAAGAAAAGTTTCTTTTAAATCGTGGAAACAAAGGGGTTGACATGATATTGGAGCAAAATGCTACCGGTGAAGACGCATTGAGAGGGTGGCTTGTTGCTGGATACGCTGCAGATATAGAGAAGTCTTTTCATGTGCCGAGCTCAAGCGCACTGGAAGAGGCTTACGAGAAGATGAATGACGTATTTGGTCCATTTGTATCGGAACTGCAGGCCAAGGGGTGGCATACCGATCGATTTCTCGATGGAACAGGAAGCCGTTTTTCGTGGGGGTCGAATGTAGCTACAGATAATAATTCTTCAAGATATTGA

Protein Analysis

438

Amino Acids

48.17

Weight (kDa)

8.43

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UVB_sens_prot PF04884 60 - 284 3.1e-64 Vitamin B6 photo-protection and homoeostasis domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 1224
AccI GTMKAC 1 cut(s) 762
AciI CCGC 2 cut(s) 503, 784
AclI AACGTT 1 cut(s) 433
AclWI GGATC 2 cut(s) 679, 692
AcsI RAATTY 2 cut(s) 203, 441
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 4 cut(s) 64, 334, 904, 1224
AflII CTTAAG 1 cut(s) 845
AgeI ACCGGT 1 cut(s) 1070
AjiI CACGTC 1 cut(s) 454
AluBI AGCT 5 cut(s) 50, 351, 508, 1146, 1286
AluI AGCT 5 cut(s) 50, 351, 508, 1146, 1286
Alw21I GWGCWC 2 cut(s) 459, 1148
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 679, 692
Ama87I CYCGRG 2 cut(s) 214, 904
AoxI GGCC 4 cut(s) 164, 594, 737, 1215
ApeKI GCWGC 7 cut(s) 253, 269, 308, 373, 505, 563, 1112
ApoI RAATTY 2 cut(s) 203, 441
AsiGI ACCGGT 1 cut(s) 1070
Asp700I GAANNNNTTC 2 cut(s) 418, 1014
AspLEI GCGC 2 cut(s) 410, 1154
AspS9I GGNCC 2 cut(s) 737, 1193
AsuC2I CCSGG 4 cut(s) 245, 869, 905, 906
AsuHPI GGTGA 3 cut(s) 197, 995, 1085
AvaI CYCGRG 2 cut(s) 214, 904
AvaII GGWCC 1 cut(s) 1193
AxyI CCTNAGG 2 cut(s) 63, 878
BaeGI GKGCMC 1 cut(s) 250
BamHI GGATCC 1 cut(s) 684
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 1148
BauI CACGAG 1 cut(s) 956
BbsI GAAGAC 2 cut(s) 897, 1083
Bbv12I GWGCWC 2 cut(s) 459, 1148
BbvI GCAGC 7 cut(s) 265, 281, 295, 385, 517, 575, 1099
BccI CCATC 4 cut(s) 359, 544, 721, 1241
BceAI ACGGC 1 cut(s) 1245
BcgI CGANNNNNNTGC 2 cut(s) 1217, 1251
BciVI GTATCC 1 cut(s) 1100
BcnI CCSGG 4 cut(s) 245, 869, 905, 906
BcoDI GTCTC 1 cut(s) 106
BfmI CTRYAG 5 cut(s) 251, 309, 1113, 1211, 1287
BfrI CTTAAG 1 cut(s) 845
BfuAI ACCTGC 1 cut(s) 515
BfuI GTATCC 1 cut(s) 1100
BisI GCNGC 7 cut(s) 254, 270, 309, 374, 506, 564, 1113
BlsI GCNGC 7 cut(s) 255, 271, 310, 375, 507, 565, 1114
Bme1390I CCNGG 4 cut(s) 245, 869, 905, 906
Bme18I GGWCC 1 cut(s) 1193
BmeT110I CYCGRG 2 cut(s) 214, 904
BmgBI CACGTC 1 cut(s) 454
BmgT120I GGNCC 2 cut(s) 737, 1193
BmiI GGNNCC 3 cut(s) 39, 69, 686
BmrFI CCNGG 4 cut(s) 245, 869, 905, 906
BmsI GCATC 4 cut(s) 15, 360, 704, 913
BpiI GAAGAC 2 cut(s) 897, 1083
BplI GAGNNNNNCTC 4 cut(s) 86, 118, 632, 664
BpmI CTGGAG 2 cut(s) 32, 36
BpuEI CTTGAG 1 cut(s) 1132
BpuMI CCSGG 4 cut(s) 245, 869, 905, 906
Bsa29I ATCGAT 1 cut(s) 1237
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 5 cut(s) 126, 213, 904, 905, 1218
BsaWI WCCGGW 1 cut(s) 1070
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 334, 904, 1224
Bse118I RCCGGY 2 cut(s) 70, 1070
Bse1I ACTGG 5 cut(s) 83, 167, 264, 478, 1161
Bse21I CCTNAGG 2 cut(s) 63, 878
Bse3DI GCAATG 1 cut(s) 831
BseCI ATCGAT 1 cut(s) 1237
BseDI CCNNGG 5 cut(s) 126, 213, 904, 905, 1218
BseGI GGATG 4 cut(s) 370, 375, 555, 928
BseLI CCNNNNNNNGG 4 cut(s) 64, 334, 904, 1224
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 4 cut(s) 54, 305, 414, 869
BseNI ACTGG 5 cut(s) 83, 167, 264, 478, 1161
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 7 cut(s) 265, 281, 295, 385, 517, 575, 1099
BseYI CCCAGC 1 cut(s) 351
BsgI GTGCAG 2 cut(s) 288, 315
Bsh1285I CGRYCG 1 cut(s) 1237
BshFI GGCC 4 cut(s) 166, 596, 739, 1217
BshNI GGYRCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 1070
BshVI ATCGAT 1 cut(s) 1237
BsiEI CGRYCG 1 cut(s) 1237
BsiHKAI GWGCWC 2 cut(s) 459, 1148
BsiHKCI CYCGRG 2 cut(s) 214, 904
BsiSI CCGG 6 cut(s) 71, 244, 869, 905, 927, 1071
BslI CCNNNNNNNGG 4 cut(s) 64, 334, 904, 1224
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 4 cut(s) 166, 596, 739, 1217
Bso31I GGTCTC 1 cut(s) 106
BsoBI CYCGRG 2 cut(s) 214, 904
Bsp1286I GDGCHC 3 cut(s) 250, 459, 1148
Bsp143I GATC 3 cut(s) 622, 684, 1234
BspACI CCGC 2 cut(s) 503, 784
BspANI GGCC 4 cut(s) 166, 596, 739, 1217
BspCNI CTCAG 4 cut(s) 55, 304, 415, 870
BspDI ATCGAT 1 cut(s) 1237
BspLI GGNNCC 3 cut(s) 39, 69, 686
BspMAI CTGCAG 4 cut(s) 255, 313, 1117, 1215
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 2 cut(s) 679, 692
BspT107I GGYRCC 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 845
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 2 cut(s) 70, 1070
BsrI ACTGG 5 cut(s) 83, 167, 264, 478, 1161
BssAI RCCGGY 2 cut(s) 70, 1070
BssECI CCNNGG 5 cut(s) 126, 213, 904, 905, 1218
BssMI GATC 3 cut(s) 622, 684, 1234
BssNAI GTATAC 1 cut(s) 763
BssSI CACGAG 1 cut(s) 956
BssT1I CCWWGG 1 cut(s) 1218
Bst1107I GTATAC 1 cut(s) 763
Bst2BI CACGAG 1 cut(s) 956
Bst4CI ACNGT 5 cut(s) 110, 343, 655, 703, 767
Bst6I CTCTTC 2 cut(s) 747, 1155
BstAFI CTTAAG 1 cut(s) 845
BstAPI GCANNNNNTGC 1 cut(s) 560
BstC8I GCNNGC 5 cut(s) 72, 313, 317, 786, 1215
BstDEI CTNAG 5 cut(s) 63, 291, 423, 617, 878
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 4 cut(s) 370, 375, 555, 928
BstHHI GCGC 2 cut(s) 410, 1154
BstKTI GATC 3 cut(s) 625, 687, 1237
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 3 cut(s) 622, 684, 1234
BstMCI CGRYCG 1 cut(s) 1237
BstMWI GCNNNNNNNGC 4 cut(s) 405, 560, 569, 1109
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 4 cut(s) 243, 867, 903, 904
BstSFI CTRYAG 5 cut(s) 251, 309, 1113, 1211, 1287
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 7 cut(s) 265, 281, 295, 385, 517, 575, 1099
BstV2I GAAGAC 2 cut(s) 897, 1083
BstX2I RGATCY 1 cut(s) 684
BstYI RGATCY 1 cut(s) 684
BstZ17I GTATAC 1 cut(s) 763
Bsu15I ATCGAT 1 cut(s) 1237
Bsu36I CCTNAGG 2 cut(s) 63, 878
BsuI GTATCC 1 cut(s) 1100
BsuRI GGCC 4 cut(s) 166, 596, 739, 1217
BsuTUI ATCGAT 1 cut(s) 1237
BtgI CCRYGG 1 cut(s) 126
BtrI CACGTC 1 cut(s) 454
BtsCI GGATG 4 cut(s) 370, 375, 555, 928
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 5 cut(s) 248, 271, 285, 471, 1154
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 5 cut(s) 72, 313, 317, 786, 1215
CfoI GCGC 2 cut(s) 410, 1154
Cfr10I RCCGGY 2 cut(s) 70, 1070
Cfr13I GGNCC 2 cut(s) 737, 1193
Cfr9I CCCGGG 1 cut(s) 904
ClaI ATCGAT 1 cut(s) 1237
CseI GACGC 1 cut(s) 1088
Csp6I GTAC 1 cut(s) 76
CspAI ACCGGT 1 cut(s) 1070
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 5 cut(s) 379, 478, 758, 1048, 1136
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 5 cut(s) 63, 291, 423, 617, 878
DpnI GATC 3 cut(s) 624, 686, 1236
DpnII GATC 3 cut(s) 622, 684, 1234
DraI TTTAAA 1 cut(s) 1023
DrdI GACNNNNNNGTC 1 cut(s) 194
DseDI GACNNNNNNGTC 1 cut(s) 194
Eam1104I CTCTTC 2 cut(s) 747, 1155
EarI CTCTTC 2 cut(s) 747, 1155
Ecl136II GAGCTC 1 cut(s) 1146
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 1 cut(s) 1148
Eco31I GGTCTC 1 cut(s) 106
Eco32I GATATC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 1193
Eco53kI GAGCTC 1 cut(s) 1146
Eco81I CCTNAGG 2 cut(s) 63, 878
Eco88I CYCGRG 2 cut(s) 214, 904
EcoICRI GAGCTC 1 cut(s) 1146
EcoRV GATATC 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 1218
EcoT38I GRGCYC 1 cut(s) 1148
ErhI CCWWGG 1 cut(s) 1218
FaeI CATG 5 cut(s) 382, 481, 761, 1051, 1139
FatI CATG 5 cut(s) 378, 477, 757, 1047, 1135
FauI CCCGC 1 cut(s) 777
FblI GTMKAC 1 cut(s) 762
Fnu4HI GCNGC 7 cut(s) 254, 270, 309, 374, 506, 564, 1113
FokI GGATG 4 cut(s) 377, 382, 562, 935
FriOI GRGCYC 1 cut(s) 1148
Fsp4HI GCNGC 7 cut(s) 254, 270, 309, 374, 506, 564, 1113
GlaI GCGC 2 cut(s) 409, 1153
GluI GCNGC 7 cut(s) 254, 270, 309, 374, 506, 564, 1113
GsaI CCCAGC 1 cut(s) 355
GsuI CTGGAG 2 cut(s) 32, 36
HaeIII GGCC 4 cut(s) 166, 596, 739, 1217
HapII CCGG 6 cut(s) 71, 244, 869, 905, 927, 1071
HgaI GACGC 1 cut(s) 1088
HhaI GCGC 2 cut(s) 410, 1154
Hin1II CATG 5 cut(s) 382, 481, 761, 1051, 1139
Hin6I GCGC 2 cut(s) 408, 1152
HinP1I GCGC 2 cut(s) 408, 1152
HincII GTYRAC 2 cut(s) 134, 1045
HindII GTYRAC 2 cut(s) 134, 1045
HinfI GANTC 7 cut(s) 95, 148, 158, 188, 419, 808, 912
HpaII CCGG 6 cut(s) 71, 244, 869, 905, 927, 1071
HphI GGTGA 3 cut(s) 197, 995, 1085
Hpy166II GTNNAC 3 cut(s) 134, 763, 1045
Hpy188I TCNGA 3 cut(s) 94, 424, 1207
Hpy188III TCNNGA 6 cut(s) 62, 152, 216, 887, 1244, 1305
Hpy8I GTNNAC 3 cut(s) 134, 763, 1045
HpyAV CCTTC 3 cut(s) 28, 939, 981
HpyCH4III ACNGT 5 cut(s) 110, 343, 655, 703, 767
HpyCH4IV ACGT 3 cut(s) 433, 453, 1185
HpyF10VI GCNNNNNNNGC 4 cut(s) 405, 560, 569, 1109
HpyF3I CTNAG 5 cut(s) 63, 291, 423, 617, 878
HpySE526I ACGT 3 cut(s) 433, 453, 1185
Hsp92II CATG 5 cut(s) 382, 481, 761, 1051, 1139
HspAI GCGC 2 cut(s) 408, 1152
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 622, 684, 1234
LmnI GCTCC 4 cut(s) 37, 55, 677, 1057
Lsp1109I GCAGC 7 cut(s) 265, 281, 295, 385, 517, 575, 1099
LweI GCATC 4 cut(s) 15, 360, 704, 913
MaeII ACGT 3 cut(s) 433, 453, 1185
MaeIII GTNAC 2 cut(s) 185, 294
MalI GATC 3 cut(s) 624, 686, 1236
MboI GATC 3 cut(s) 622, 684, 1234
MflI RGATCY 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 250, 459, 1148
MluCI AATT 8 cut(s) 203, 239, 441, 535, 585, 689, 986, 1297
MlyI GAGTC 3 cut(s) 104, 182, 906
MmeI TCCRAC 1 cut(s) 416
MroNI GCCGGC 1 cut(s) 70
MroXI GAANNNNTTC 2 cut(s) 418, 1014
MseI TTAA 4 cut(s) 260, 846, 966, 1022
MslI CAYNNNNRTG 1 cut(s) 476
MspCI CTTAAG 1 cut(s) 845
MspI CCGG 6 cut(s) 71, 244, 869, 905, 927, 1071
MspR9I CCNGG 4 cut(s) 245, 869, 905, 906
MwoI GCNNNNNNNGC 4 cut(s) 405, 560, 569, 1109
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 4 cut(s) 245, 869, 905, 906
NdeII GATC 3 cut(s) 622, 684, 1234
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 5 cut(s) 382, 481, 761, 1051, 1139
NlaIV GGNNCC 3 cut(s) 39, 69, 686
NmeAIII GCCGAG 1 cut(s) 1167
NmuCI GTSAC 2 cut(s) 185, 294
NspI RCATGY 1 cut(s) 761
PcsI WCGNNNNNNNCGW 1 cut(s) 1274
PdiI GCCGGC 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 418, 1014
PfeI GAWTC 4 cut(s) 148, 158, 419, 808
PflMI CCANNNNNTGG 1 cut(s) 1224
PinAI ACCGGT 1 cut(s) 1070
PkrI GCNGC 7 cut(s) 255, 271, 310, 375, 507, 565, 1114
Ple19I CGATCG 1 cut(s) 1237
PleI GAGTC 3 cut(s) 103, 182, 906
PpsI GAGTC 3 cut(s) 103, 182, 906
Psp124BI GAGCTC 1 cut(s) 1148
Psp1406I AACGTT 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 39, 69, 686
PspPI GGNCC 2 cut(s) 737, 1193
PsrI GAACNNNNNNTAC 2 cut(s) 339, 371
PstI CTGCAG 4 cut(s) 255, 313, 1117, 1215
PsuI RGATCY 1 cut(s) 684
PvuI CGATCG 1 cut(s) 1237
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 1148
SaqAI TTAA 4 cut(s) 260, 846, 966, 1022
SatI GCNGC 7 cut(s) 254, 270, 309, 374, 506, 564, 1113
Sau3AI GATC 3 cut(s) 622, 684, 1234
Sau96I GGNCC 2 cut(s) 737, 1193
SchI GAGTC 3 cut(s) 104, 182, 906
ScrFI CCNGG 4 cut(s) 245, 869, 905, 906
SduI GDGCHC 3 cut(s) 250, 459, 1148
SfaNI GCATC 4 cut(s) 15, 360, 704, 913
SfcI CTRYAG 5 cut(s) 251, 309, 1113, 1211, 1287
SinI GGWCC 1 cut(s) 1193
SmaI CCCGGG 1 cut(s) 906
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 845, 1147
SmoI CTYRAG 2 cut(s) 845, 1147
Sse9I AATT 8 cut(s) 203, 239, 441, 535, 585, 689, 986, 1297
SsiI CCGC 2 cut(s) 503, 784
SspI AATATT 1 cut(s) 999
SstI GAGCTC 1 cut(s) 1148
StyD4I CCNGG 4 cut(s) 243, 867, 903, 904
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 5 cut(s) 110, 343, 655, 703, 767
TaiI ACGT 3 cut(s) 436, 456, 1188
TaqI TCGA 4 cut(s) 161, 1237, 1245, 1277
TaqII GACCGA 1 cut(s) 88
TasI AATT 8 cut(s) 203, 239, 441, 535, 585, 689, 986, 1297
TfiI GAWTC 4 cut(s) 148, 158, 419, 808
Tru1I TTAA 4 cut(s) 260, 846, 966, 1022
Tru9I TTAA 4 cut(s) 260, 846, 966, 1022
TscAI CASTG 5 cut(s) 255, 271, 285, 478, 1161
TseFI GTSAC 2 cut(s) 185, 294
TseI GCWGC 7 cut(s) 253, 269, 308, 373, 505, 563, 1112
Tsp45I GTSAC 2 cut(s) 185, 294
TspDTI ATGAA 3 cut(s) 17, 1124, 1193
TspGWI ACGGA 1 cut(s) 164
TspMI CCCGGG 1 cut(s) 904
TspRI CASTG 5 cut(s) 255, 271, 285, 478, 1161
Van91I CCANNNNNTGG 1 cut(s) 1224
Vha464I CTTAAG 1 cut(s) 845
VpaK11BI GGWCC 1 cut(s) 1193
XapI RAATTY 2 cut(s) 203, 441
XceI RCATGY 1 cut(s) 761
XmaI CCCGGG 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 2 cut(s) 418, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.