Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007946

Physical Location & Seq

Genomic Coordinates Forward (+)

34970172 .. 34979679

Gene Structure 9508 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1219300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGAGACCTTCAATTTCAAAGCAGGGAACTGCAGACAATAGTAATAAGGGCCTCACCGGTCACACCTGCACCCTCCATCAGCGTCTCAATCATGCTCTGAACCTCGGCACCAGAATTAATCACGACAAGGTGAAAAAATGGCAGTGTGTCGATATCGAGATACAAAGACATGTGCTCCACTCAATAGCTGCATTTCTCGATTCTGTTTCTGGGGATACGCGGCACCATCCACATGTGAAGGCTTCAGTTGTTGACATTGTTGGCGCATTGGTTTGGATGCTTCAATCTGACAATGGATCCATGTTGAGGATGGCAGCGGATGTGACAGTAAAATTAGTCAGTATTTTCCCCAAATCAGTACTGCAGCTTTATGTGTTAGAGCTTGTTAATCCTTTGTCATCTTTGTTATCTTCGCACCAAACAGAAGTAGCTATTTTATCTGCAACAGCATTGAATCTTATTCTTTCAAACCTCAGTACCAAGAGTGAGAAAGACGTTTGGGAAATTCTTAGGAAAACAGAAAGTGTATCTCAAGTTGTCAGTAATTTACGGTTTTTTCCTGGATGTACAAAGACACTGAAATATTTCCAACAGATGGCTTTACTTTTGAGCACAATACTATGGTGGTGGCCGTCATCCAGGTTTTCTGTTTGGAGTGATACTAAACTGATGAATGGCTTAAATGAAATGTTAAAGCTAGATATTGATGAAAAAGTTGCAGTTTTGAAGTTGTATTCTTCTTTAGCTTTATGTGGTATTGGGGCCAAAAAAGTATTGGAAAGTGGAGAGATCCTCGAACAGATGGTGCAATGCATGGACAACTCTCATCCACACTCAATTCGCATTGAAGGATTCAAGTTAGCCCAGTGCCTAGTGATAAATGAAGAAATATGCTTACTAATGATGAGCTTAGGTTACAAACCTATCATCAAAGCCATAATTTCTGGAATGAGTGAGTGGAACTCACATTCTAGAAAGGTCAGCAATGACCAGATGTCTTTGCTAGAGGAGGCATGTCGCTTGGCTCTGATAACTCGTTGGGAAGGGGAGCATCACATTTATATGTGGAAACAAGGGATTGACAAAATCCTTCTTGATCTTCTCCTTGAAAATTTTCACAACCAACCATACAAATGTTCCATGTCATTGGATGAGCAAATGGTTATTGCCAAGGAGGGTCTCAATGCCAATTATCTTCTTGTTCTGAGGAGTTATATCTGGGATATTCTTGGATGGCTTGCAATACATTGTGGGGAGGATTTCAACCCTGAAAGGGAAATCTATATTAACATCCTCATCACATGTGCTTGTTTGACCTTTGTTGATGCAATTCAAAAGTGGCATAAAATATATGAAAATGATATTGCTGGTGCCTTCAGACGTGAATCGGCTACAAGGGCAGTTTCAATGATGATTTATTCACCCTGTAAATACATTGCGTCGAAGACCAGATTCATATTGTCCGAAATACTAGGACTAGATGGAGGGGAGTATTTGAAAACCTTACTGGGTTTCCTAAATAATTTGTCATCTGGGAATTATTTTGGAGTTCCAGATAAACTCCAAATTATTGTTTATTTAATGGGATTAGCATGCTATTCAGCTTTGCCACAGTACCAAGTCTCAGGTAAAAAATGTGTAAAAACATTATTGGCTTTTATAAGGTGGTGCTTAACTAATAATTTCCATTTAGAAAGATCAAGCTTTGCTCCTCATCTGCATAATCCATTCCATGAGAGGATTTGCTGTTGGGTTTCTGCAGAAGACTGGGAAGGCAAAGACATTCTTTTGTTTTACAGTTTGTGGGGGCTGGCTGAGTGGATACAGCATTGGGGATGCTTAGGAAATAACCTAGACCAAACTTCTGGTGAGATGAGACCAATAGAAGCTCAACTTGTTAGTGAACTTCAGGATATCTGTAACAACAGTCGTACTCCTGGAGTGCAATGGTATGCTGCATATGTTCTTAGTTATTTTGGATATTATGGTTTTCCAAGTAAACTTGGGAAAAAGATTGGCAAGGCACTTACTGAGAAGGATTATGCTGACATACAACTCATTCTTGCAAATGGAGAGTGTTTGAGGGTCCATGGTGTTGTGCTTGCAACTCAATGTCCATCATTGTTGCCTTCTGAAGTGCTGTTTCCCAGCGAGGTAACATGTGATGAATCTTCAGTGACATGCTCTATGGAAATCTGTAAAAAGTTCTGTAAAGACATAAGGTTATCTGTTCATGTTGATCACCAAGCGTTGGTTATGTTGTTGGAGTATATCTATTTGGGATATCTACAAGCAGGACAAGAACTTGTAAAGAAGTTGAGGACTCTTGCCAAACGTTGTAATCTACAGTCTTTGTTACAGATGCTTTATAGGAAACGTCCCAAGTGGGGCTCTGCTCACCCCAGCCTTGATCTTAGTGCAGCTCTTCGTCTGTCTGGGTGTCGTTTCTCAGATGTCATCTTAGAAGCTAAGACAACTGAGCTTTTGGGCTGGACATGTGGTATTTGCTTTCGCTCGGTGCCACATATGCACGTTCACAAAGTTATTTTGTCATCAAGTTGTGATTATTTGCGGGCCTTGTTACAGTCAGGAATGCAAGAGAGCCATTCACAAACCATAAAGAGCTCTATTAGTTGGGAAGCAATGGTTAAACTAGTCAACTGCTTCTATTGTGGTAAGCTGCCAAAGCCTCCCTCTGGATGTTTATGGAATAATATGGACACCGAAGAAAAGTTACATGAGCTGCAGCCATATGTAGAGCTCTGTTGGCTTTCTGAGTTCTGGCTCATGGAAGATATTCAGACAGCATGCTTCGATGTGATCATATCAACTCTCAGTTCTGCCAAAGAGTTGTCTATTAAGATACTACAAATTGCTGCCAATTTTTCTCTGTGGAAGTTGGCCGAAGTTGCAGCAACTTATGCAGCCCCTTTATATCGCCAACTGTGTGATTCTGGTGAACTCGACGCACTTGATGAAATGCTGGTTGAGATGATTCGTGCAGCCTCTGTTCATCTCTCTCAACAGGGTGGTAATCAATGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB

Protein Analysis

1015

Amino Acids

114.67

Weight (kDa)

6.71

Isoelectric Point (pI)

45.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_6	PF26522	138 - 251	6.7e-45	Plant ARM repeat
BTB	PF00651	842 - 899	1.2e-08	BTB/POZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04390
fragaria_vesca	FvH4_3g15860
malus_domestica	MD05G1219300.v1.1
prunus_persica	Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1
pyrus_communis	pycom05g20050 pycom05g20060
rosa_chinensis	RchiOBHm_Chr5g0026671
rosa_laevigata	RLG00000032945
rosa_multiflora	Rmu_co8501061.1_g000001 Rmu_sc0004490.1_g000009
rosa_roxburghii	Rroxscaffold_1G00052790
rosa_rugosa	Rorug05G0094700 Rorug05G0094700
rosa_samantha	Rh5AG188300 Rh5BG184600 Rh5CG204500 Rh5DG186900
rosa_wichuraiana	Rw5G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1661
AarI	CACCTGC	1 cut(s)	74
Acc36I	ACCTGC	1 cut(s)	74
AccB1I	GGYRCC	4 cut(s)	107, 222, 1370, 2518
AccB7I	CCANNNNNTGG	2 cut(s)	595, 2251
AccII	CGCG	1 cut(s)	220
AciI	CCGC	3 cut(s)	220, 317, 2572
AclI	AACGTT	1 cut(s)	2335
AclWI	GGATC	3 cut(s)	291, 304, 784
AcoI	YGGCCR	2 cut(s)	629, 2901
AcsI	RAATTY	2 cut(s)	504, 1111
AcuI	CTGAAG	5 cut(s)	228, 1360, 1892, 2154, 2157
AfaI	GTAC	5 cut(s)	360, 478, 568, 1616, 1933
AfiI	CCNNNNNNNGG	6 cut(s)	306, 595, 1273, 2251, 2387, 2696
AflIII	ACRYGT	5 cut(s)	169, 232, 1301, 2159, 2495
AgeI	ACCGGT	1 cut(s)	56
AhdI	GACNNNNNGTC	1 cut(s)	994
AhlI	ACTAGT	1 cut(s)	2653
AjiI	CACGTC	1 cut(s)	1382
AjnI	CCWGG	3 cut(s)	559, 638, 1936
Alw21I	GWGCWC	4 cut(s)	177, 614, 2627, 2763
Alw26I	GTCTC	4 cut(s)	89, 1184, 1627, 1870
AlwI	GGATC	3 cut(s)	291, 304, 784
AlwNI	CAGNNNCTG	1 cut(s)	3008
AoxI	GGCC	5 cut(s)	49, 629, 762, 2574, 2901
ApoI	RAATTY	2 cut(s)	504, 1111
AseI	ATTAAT	1 cut(s)	117
AsiGI	ACCGGT	1 cut(s)	56
Asp700I	GAANNNNTTC	3 cut(s)	584, 1113, 2796
AspLEI	GCGC	1 cut(s)	266
AspS9I	GGNCC	4 cut(s)	49, 762, 2086, 2574
AsuHPI	GGTGA	7 cut(s)	46, 142, 1413, 1880, 2234, 2390, 2969
AvaII	GGWCC	1 cut(s)	2086
BaeI	ACNNNNGTAYC	2 cut(s)	460, 493
BamHI	GGATCC	1 cut(s)	296
BanI	GGYRCC	4 cut(s)	107, 222, 1370, 2518
BanII	GRGCYC	3 cut(s)	2393, 2627, 2763
BarI	GAAGNNNNNNTAC	2 cut(s)	2718, 2750
BbsI	GAAGAC	2 cut(s)	1451, 1770
Bbv12I	GWGCWC	4 cut(s)	177, 614, 2627, 2763
BccI	CCATC	8 cut(s)	84, 234, 304, 589, 796, 1227, 1475, 2125
BceAI	ACGGC	1 cut(s)	616
BciT130I	CCWGG	3 cut(s)	561, 640, 1938
BciVI	GTATCC	2 cut(s)	208, 1815
BclI	TGATCA	2 cut(s)	2239, 2820
BcoDI	GTCTC	4 cut(s)	89, 1184, 1627, 1870
BcuI	ACTAGT	1 cut(s)	2653
BfaI	CTAG	8 cut(s)	698, 872, 972, 1004, 1472, 1478, 1853, 2654
BfmI	CTRYAG	5 cut(s)	30, 362, 1758, 2345, 2744
BfuAI	ACCTGC	1 cut(s)	74
BfuI	GTATCC	2 cut(s)	208, 1815
BmcAI	AGTACT	1 cut(s)	360
Bme1390I	CCNGG	3 cut(s)	561, 640, 1938
Bme18I	GGWCC	1 cut(s)	2086
BmeRI	GACNNNNNGTC	1 cut(s)	994
BmgBI	CACGTC	1 cut(s)	1382
BmgT120I	GGNCC	4 cut(s)	49, 762, 2086, 2574
BmiI	GGNNCC	7 cut(s)	109, 224, 298, 763, 1372, 2087, 2520
BmrFI	CCNGG	3 cut(s)	561, 640, 1938
BmrI	ACTGGG	3 cut(s)	859, 1517, 1777
BmsI	GCATC	5 cut(s)	267, 1060, 1315, 1826, 2352
BmuI	ACTGGG	3 cut(s)	859, 1517, 1777
BpiI	GAAGAC	2 cut(s)	1451, 1770
BplI	GAGNNNNNCTC	4 cut(s)	777, 809, 947, 979
BpmI	CTGGAG	1 cut(s)	1959
Bpu10I	CCTNAGC	2 cut(s)	910, 1840
BpuEI	CTTGAG	1 cut(s)	516
BsaI	GGTCTC	2 cut(s)	1184, 1870
BsaJI	CCNNGG	3 cut(s)	103, 1170, 2089
BsaWI	WCCGGW	1 cut(s)	56
BsaXI	ACNNNNNCTCC	2 cut(s)	159, 189
Bsc4I	CCNNNNNNNGG	6 cut(s)	306, 595, 1273, 2251, 2387, 2696
Bse118I	RCCGGY	1 cut(s)	56
Bse1I	ACTGG	3 cut(s)	865, 1512, 1772
Bse3DI	GCAATG	5 cut(s)	815, 991, 1434, 1952, 2649
BseBI	CCWGG	3 cut(s)	561, 640, 1938
BseDI	CCNNGG	3 cut(s)	103, 1170, 2089
BseLI	CCNNNNNNNGG	6 cut(s)	306, 595, 1273, 2251, 2387, 2696
BseMI	GCAATG	5 cut(s)	815, 991, 1434, 1952, 2649
BseMII	CTCAG	9 cut(s)	487, 1196, 1638, 1806, 2022, 2463, 2469, 2766, 2848
BseNI	ACTGG	3 cut(s)	865, 1512, 1772
BseRI	GAGGAG	3 cut(s)	1022, 1222, 1701
BseYI	CCCAGC	2 cut(s)	2147, 2402
BsgI	GTGCAG	3 cut(s)	52, 2439, 3021
Bsh1236I	CGCG	1 cut(s)	220
BshFI	GGCC	5 cut(s)	51, 631, 764, 2576, 2903
BshNI	GGYRCC	4 cut(s)	107, 222, 1370, 2518
BshTI	ACCGGT	1 cut(s)	56
BsiHKAI	GWGCWC	4 cut(s)	177, 614, 2627, 2763
BsiSI	CCGG	1 cut(s)	57
BslFI	GGGAC	1 cut(s)	2364
BslI	CCNNNNNNNGG	6 cut(s)	306, 595, 1273, 2251, 2387, 2696
BsmAI	GTCTC	4 cut(s)	89, 1184, 1627, 1870
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	1 cut(s)	2364
BsmI	GAATGC	1 cut(s)	2598
BsnI	GGCC	5 cut(s)	51, 631, 764, 2576, 2903
Bso31I	GGTCTC	2 cut(s)	1184, 1870
Bsp1286I	GDGCHC	5 cut(s)	177, 614, 2393, 2627, 2763
Bsp1407I	TGTACA	1 cut(s)	566
Bsp143I	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
Bsp19I	CCATGG	1 cut(s)	2089
BspACI	CCGC	3 cut(s)	220, 317, 2572
BspANI	GGCC	5 cut(s)	51, 631, 764, 2576, 2903
BspCNI	CTCAG	9 cut(s)	486, 1197, 1637, 1807, 2023, 2462, 2470, 2767, 2847
BspFNI	CGCG	1 cut(s)	220
BspLI	GGNNCC	7 cut(s)	109, 224, 298, 763, 1372, 2087, 2520
BspMAI	CTGCAG	4 cut(s)	34, 366, 1762, 2748
BspMI	ACCTGC	1 cut(s)	74
BspPI	GGATC	3 cut(s)	291, 304, 784
BspQI	GCTCTTC	1 cut(s)	2430
BspT107I	GGYRCC	4 cut(s)	107, 222, 1370, 2518
BspTNI	GGTCTC	2 cut(s)	1184, 1870
BsrDI	GCAATG	5 cut(s)	815, 991, 1434, 1952, 2649
BsrFI	RCCGGY	1 cut(s)	56
BsrGI	TGTACA	1 cut(s)	566
BsrI	ACTGG	3 cut(s)	865, 1512, 1772
BssAI	RCCGGY	1 cut(s)	56
BssECI	CCNNGG	3 cut(s)	103, 1170, 2089
BssMI	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
BssT1I	CCWWGG	2 cut(s)	1170, 2089
Bst2UI	CCWGG	3 cut(s)	561, 640, 1938
Bst4CI	ACNGT	8 cut(s)	328, 552, 1614, 1799, 1928, 2349, 2586, 2946
Bst6I	CTCTTC	1 cut(s)	2430
BstAPI	GCANNNNNTGC	1 cut(s)	2921
BstAUI	TGTACA	1 cut(s)	566
BstC8I	GCNNGC	6 cut(s)	1239, 1594, 1812, 2103, 2574, 2809
BstDSI	CCRYGG	1 cut(s)	2089
BstFNI	CGCG	1 cut(s)	220
BstHHI	GCGC	1 cut(s)	266
BstKTI	GATC	7 cut(s)	299, 792, 1099, 1700, 2242, 2413, 2823
BstMAI	GTCTC	4 cut(s)	89, 1184, 1627, 1870
BstMBI	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
BstMWI	GCNNNNNNNGC	6 cut(s)	1397, 2527, 2686, 2767, 2909, 2921
BstNI	CCWGG	3 cut(s)	561, 640, 1938
BstNSI	RCATGY	9 cut(s)	173, 236, 1017, 1305, 1596, 2163, 2184, 2499, 2811
BstSCI	CCNGG	3 cut(s)	559, 638, 1936
BstSFI	CTRYAG	5 cut(s)	30, 362, 1758, 2345, 2744
BstUI	CGCG	1 cut(s)	220
BstV2I	GAAGAC	2 cut(s)	1451, 1770
BstX2I	RGATCY	2 cut(s)	296, 789
BstXI	CCANNNNNNTGG	2 cut(s)	1147, 1865
BstYI	RGATCY	2 cut(s)	296, 789
BsuI	GTATCC	2 cut(s)	208, 1815
BsuRI	GGCC	5 cut(s)	51, 631, 764, 2576, 2903
BtgI	CCRYGG	1 cut(s)	2089
BtrI	CACGTC	1 cut(s)	1382
BtsI	GCAGTG	1 cut(s)	149
BtsIMutI	CAGTG	4 cut(s)	149, 575, 872, 2181
BveI	ACCTGC	1 cut(s)	74
Cac8I	GCNNGC	6 cut(s)	1239, 1594, 1812, 2103, 2574, 2809
CaiI	CAGNNNCTG	1 cut(s)	3008
CfoI	GCGC	1 cut(s)	266
Cfr10I	RCCGGY	1 cut(s)	56
Cfr13I	GGNCC	4 cut(s)	49, 762, 2086, 2574
CseI	GACGC	3 cut(s)	71, 1428, 2975
Csp6I	GTAC	5 cut(s)	359, 477, 567, 1615, 1932
CspAI	ACCGGT	1 cut(s)	56
CviQI	GTAC	5 cut(s)	359, 477, 567, 1615, 1932
DpnI	GATC	7 cut(s)	298, 791, 1098, 1699, 2241, 2412, 2822
DpnII	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
DriI	GACNNNNNGTC	1 cut(s)	994
EaeI	YGGCCR	2 cut(s)	629, 2901
Eam1104I	CTCTTC	1 cut(s)	2430
Eam1105I	GACNNNNNGTC	1 cut(s)	994
EarI	CTCTTC	1 cut(s)	2430
Ecl136II	GAGCTC	2 cut(s)	2625, 2761
Eco130I	CCWWGG	2 cut(s)	1170, 2089
Eco24I	GRGCYC	3 cut(s)	2393, 2627, 2763
Eco31I	GGTCTC	2 cut(s)	1184, 1870
Eco32I	GATATC	3 cut(s)	154, 1915, 2285
Eco47I	GGWCC	1 cut(s)	2086
Eco53kI	GAGCTC	2 cut(s)	2625, 2761
Eco57I	CTGAAG	5 cut(s)	228, 1360, 1892, 2154, 2157
EcoICRI	GAGCTC	2 cut(s)	2625, 2761
EcoO109I	RGGNCCY	1 cut(s)	49
EcoRII	CCWGG	3 cut(s)	559, 638, 1936
EcoRV	GATATC	3 cut(s)	154, 1915, 2285
EcoT14I	CCWWGG	2 cut(s)	1170, 2089
EcoT22I	ATGCAT	1 cut(s)	815
EcoT38I	GRGCYC	3 cut(s)	2393, 2627, 2763
ErhI	CCWWGG	2 cut(s)	1170, 2089
Esp3I	CGTCTC	1 cut(s)	89
FalI	AAGNNNNNCTT	4 cut(s)	1770, 1802, 1878, 1910
FaqI	GGGAC	1 cut(s)	2364
FauI	CCCGC	1 cut(s)	2565
FauNDI	CATATG	3 cut(s)	1960, 2526, 2752
FbaI	TGATCA	2 cut(s)	2239, 2820
FriOI	GRGCYC	3 cut(s)	2393, 2627, 2763
FspBI	CTAG	8 cut(s)	698, 872, 972, 1004, 1472, 1478, 1853, 2654
GlaI	GCGC	1 cut(s)	265
GsaI	CCCAGC	2 cut(s)	2151, 2406
GsuI	CTGGAG	1 cut(s)	1959
HaeIII	GGCC	5 cut(s)	51, 631, 764, 2576, 2903
HapII	CCGG	1 cut(s)	57
HgaI	GACGC	3 cut(s)	71, 1428, 2975
HhaI	GCGC	1 cut(s)	266
Hin6I	GCGC	1 cut(s)	264
HinP1I	GCGC	1 cut(s)	264
HincII	GTYRAC	2 cut(s)	253, 2659
HindII	GTYRAC	2 cut(s)	253, 2659
HindIII	AAGCTT	1 cut(s)	1702
HinfI	GANTC	9 cut(s)	200, 454, 852, 1385, 1452, 2168, 2323, 2951, 2995
HpaII	CCGG	1 cut(s)	57
HphI	GGTGA	7 cut(s)	46, 142, 1413, 1880, 2234, 2390, 2969
Hpy166II	GTNNAC	6 cut(s)	253, 1904, 2000, 2536, 2659, 2960
Hpy8I	GTNNAC	6 cut(s)	253, 1904, 2000, 2536, 2659, 2960
Hpy99I	CGWCG	2 cut(s)	1444, 2969
HpyAV	CCTTC	9 cut(s)	18, 232, 842, 1037, 1100, 1384, 1766, 2029, 2139
HpyCH4III	ACNGT	8 cut(s)	328, 552, 1614, 1799, 1928, 2349, 2586, 2946
HpyCH4IV	ACGT	5 cut(s)	495, 1381, 2335, 2377, 2532
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1397, 2527, 2686, 2767, 2909, 2921
HpySE526I	ACGT	5 cut(s)	495, 1381, 2335, 2377, 2532
HspAI	GCGC	1 cut(s)	264
Ksp22I	TGATCA	2 cut(s)	2239, 2820
Kzo9I	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
LguI	GCTCTTC	1 cut(s)	2430
LmnI	GCTCC	3 cut(s)	180, 1048, 1714
LweI	GCATC	5 cut(s)	267, 1060, 1315, 1826, 2352
MaeI	CTAG	8 cut(s)	698, 872, 972, 1004, 1472, 1478, 1853, 2654
MaeII	ACGT	5 cut(s)	495, 1381, 2335, 2377, 2532
MaeIII	GTNAC	9 cut(s)	59, 322, 914, 1919, 2155, 2176, 2355, 2580, 2733
MalI	GATC	7 cut(s)	298, 791, 1098, 1699, 2241, 2412, 2822
MboI	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
MflI	RGATCY	2 cut(s)	296, 789
MhlI	GDGCHC	5 cut(s)	177, 614, 2393, 2627, 2763
MlyI	GAGTC	1 cut(s)	2317
MmeI	TCCRAC	2 cut(s)	613, 2244
Mph1103I	ATGCAT	1 cut(s)	815
MroXI	GAANNNNTTC	3 cut(s)	584, 1113, 2796
MseI	TTAA	9 cut(s)	117, 387, 680, 692, 1287, 1580, 1673, 2649, 2859
MslI	CAYNNNNRTG	3 cut(s)	231, 1061, 1132
MspA1I	CMGCKG	1 cut(s)	317
MspI	CCGG	1 cut(s)	57
MspR9I	CCNGG	3 cut(s)	561, 640, 1938
Mva1269I	GAATGC	1 cut(s)	2598
MvaI	CCWGG	3 cut(s)	561, 640, 1938
MvnI	CGCG	1 cut(s)	220
MwoI	GCNNNNNNNGC	6 cut(s)	1397, 2527, 2686, 2767, 2909, 2921
NcoI	CCATGG	1 cut(s)	2089
NdeI	CATATG	3 cut(s)	1960, 2526, 2752
NdeII	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
NlaIV	GGNNCC	7 cut(s)	109, 224, 298, 763, 1372, 2087, 2520
NmeAIII	GCCGAG	1 cut(s)	84
NmuCI	GTSAC	3 cut(s)	59, 322, 2176
NsiI	ATGCAT	1 cut(s)	815
NspI	RCATGY	9 cut(s)	173, 236, 1017, 1305, 1596, 2163, 2184, 2499, 2811
PaeI	GCATGC	2 cut(s)	1596, 2811
PaqCI	CACCTGC	1 cut(s)	74
PciI	ACATGT	5 cut(s)	169, 232, 1301, 2159, 2495
PciSI	GCTCTTC	1 cut(s)	2430
PctI	GAATGC	1 cut(s)	2598
PdmI	GAANNNNTTC	3 cut(s)	584, 1113, 2796
PfeI	GAWTC	8 cut(s)	200, 454, 852, 1385, 1452, 2168, 2951, 2995
PflMI	CCANNNNNTGG	2 cut(s)	595, 2251
PfoI	TCCNGGA	2 cut(s)	559, 1936
PinAI	ACCGGT	1 cut(s)	56
PleI	GAGTC	1 cut(s)	2317
PpsI	GAGTC	1 cut(s)	2317
PscI	ACATGT	5 cut(s)	169, 232, 1301, 2159, 2495
PshBI	ATTAAT	1 cut(s)	117
PsiI	TTATAA	1 cut(s)	1661
Psp124BI	GAGCTC	2 cut(s)	2627, 2763
Psp1406I	AACGTT	1 cut(s)	2335
Psp6I	CCWGG	3 cut(s)	559, 638, 1936
PspFI	CCCAGC	2 cut(s)	2147, 2402
PspGI	CCWGG	3 cut(s)	559, 638, 1936
PspN4I	GGNNCC	7 cut(s)	109, 224, 298, 763, 1372, 2087, 2520
PspPI	GGNCC	4 cut(s)	49, 762, 2086, 2574
PstI	CTGCAG	4 cut(s)	34, 366, 1762, 2748
PstNI	CAGNNNCTG	1 cut(s)	3008
PsuI	RGATCY	2 cut(s)	296, 789
RsaI	GTAC	5 cut(s)	360, 478, 568, 1616, 1933
RsaNI	GTAC	5 cut(s)	359, 477, 567, 1615, 1932
RseI	CAYNNNNRTG	3 cut(s)	231, 1061, 1132
SacI	GAGCTC	2 cut(s)	2627, 2763
SapI	GCTCTTC	1 cut(s)	2430
SaqAI	TTAA	9 cut(s)	117, 387, 680, 692, 1287, 1580, 1673, 2649, 2859
Sau3AI	GATC	7 cut(s)	296, 789, 1096, 1697, 2239, 2410, 2820
Sau96I	GGNCC	4 cut(s)	49, 762, 2086, 2574
ScaI	AGTACT	1 cut(s)	360
SchI	GAGTC	1 cut(s)	2317
ScrFI	CCNGG	3 cut(s)	561, 640, 1938
SduI	GDGCHC	5 cut(s)	177, 614, 2393, 2627, 2763
SfaNI	GCATC	5 cut(s)	267, 1060, 1315, 1826, 2352
SfcI	CTRYAG	5 cut(s)	30, 362, 1758, 2345, 2744
SinI	GGWCC	1 cut(s)	2086
SmiMI	CAYNNNNRTG	3 cut(s)	231, 1061, 1132
SmlI	CTYRAG	1 cut(s)	531
SmoI	CTYRAG	1 cut(s)	531
SpeI	ACTAGT	1 cut(s)	2653
SphI	GCATGC	2 cut(s)	1596, 2811
SsiI	CCGC	3 cut(s)	220, 317, 2572
SspI	AATATT	1 cut(s)	584
SspMI	CTAG	8 cut(s)	698, 872, 972, 1004, 1472, 1478, 1853, 2654
SstI	GAGCTC	2 cut(s)	2627, 2763
StyD4I	CCNGG	3 cut(s)	559, 638, 1936
StyI	CCWWGG	2 cut(s)	1170, 2089
TaaI	ACNGT	8 cut(s)	328, 552, 1614, 1799, 1928, 2349, 2586, 2946
TaiI	ACGT	5 cut(s)	498, 1384, 2338, 2380, 2535
TaqI	TCGA	7 cut(s)	150, 156, 198, 795, 1442, 2814, 2964
TatI	WGTACW	2 cut(s)	358, 566
TauI	GCSGC	1 cut(s)	223
TfiI	GAWTC	8 cut(s)	200, 454, 852, 1385, 1452, 2168, 2951, 2995
Tru1I	TTAA	9 cut(s)	117, 387, 680, 692, 1287, 1580, 1673, 2649, 2859
Tru9I	TTAA	9 cut(s)	117, 387, 680, 692, 1287, 1580, 1673, 2649, 2859
TscAI	CASTG	4 cut(s)	149, 582, 872, 2181
TseFI	GTSAC	3 cut(s)	59, 322, 2176
Tsp45I	GTSAC	3 cut(s)	59, 322, 2176
TspRI	CASTG	4 cut(s)	149, 582, 872, 2181
Van91I	CCANNNNNTGG	2 cut(s)	595, 2251
VpaK11BI	GGWCC	1 cut(s)	2086
VspI	ATTAAT	1 cut(s)	117
XapI	RAATTY	2 cut(s)	504, 1111
XbaI	TCTAGA	1 cut(s)	971
XceI	RCATGY	9 cut(s)	173, 236, 1017, 1305, 1596, 2163, 2184, 2499, 2811
XcmI	CCANNNNNNNNNTGG	2 cut(s)	307, 772
XmnI	GAANNNNTTC	3 cut(s)	584, 1113, 2796
XspI	CTAG	8 cut(s)	698, 872, 972, 1004, 1472, 1478, 1853, 2654
ZrmI	AGTACT	1 cut(s)	360
Zsp2I	ATGCAT	1 cut(s)	815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.