pycom05g20060

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007946
Physical Location & Seq
Forward (+)
22958076 .. 22959442
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20060.1

Sequence Viewer

Length: 519 bp
ATGCACGTTCACAAAGTTATATTGTCATCAAGTTGTGATTATTTGCGGGCCTTGTTACAGTCAGGAATGCAAGAAAGCCATTCACAAACCGTAAAGAGCTCTATTAGTTGGGAAGCAATGGTTAAACTAGTGAACTGCTTCTATTGTGGTACGCTGCCAAAGCCTCCCTCTGGATGTTTATGGGATAATATGGACACCGAAGAAAAGTTACATGAGCTGCAGCCATATGTAGAGCTTTGTTGGCTTTCTGAGTTCTGGCTCATGGAAGATATTCAAACAGCATGCTTTGATGTGATCATATCAACTCTCGGTTCTGCCAAAGAGTTGTCTATTAAGATACTACAAATTGCTGCCAATTTTTCTCTGTGGAAATTGGCTGAAGTTGCAGCAACTTATGCTGCCCCTTTATATCGCCAACTGTGTGATTCTGGTGAACTTGACGCACTTGATGAAATGCTGGTTGAGATGATTCGTGCAGCCTCTGTTCATCTCTCTCAACAGGGTGGTAATCAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

173

Amino Acids

19.28

Weight (kDa)

4.8

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 2 - 57 3.2e-09 BTB/POZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 1 cut(s) 275
AhlI ACTAGT 1 cut(s) 127
AluBI AGCT 3 cut(s) 99, 217, 235
AluI AGCT 3 cut(s) 99, 217, 235
Alw21I GWGCWC 1 cut(s) 101
AlwNI CAGNNNCTG 1 cut(s) 482
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 7 cut(s) 154, 217, 220, 350, 386, 398, 476
Asp700I GAANNNNTTC 2 cut(s) 137, 270
AspS9I GGNCC 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 443
BanII GRGCYC 1 cut(s) 101
BarI GAAGNNNNNNTAC 2 cut(s) 192, 224
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 7 cut(s) 141, 204, 232, 337, 385, 398, 488
BclI TGATCA 1 cut(s) 294
BcuI ACTAGT 1 cut(s) 127
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 218
BisI GCNGC 7 cut(s) 155, 218, 221, 351, 387, 399, 477
BlsI GCNGC 7 cut(s) 156, 219, 222, 352, 388, 400, 478
BmgT120I GGNCC 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 123
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 240
BseXI GCAGC 7 cut(s) 141, 204, 232, 337, 385, 398, 488
BsgI GTGCAG 1 cut(s) 495
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 170
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 46
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 123
BssMI GATC 1 cut(s) 294
Bst4CI ACNGT 3 cut(s) 60, 91, 420
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 48, 283
BstDEI CTNAG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 4 cut(s) 160, 241, 383, 395
BstNSI RCATGY 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 218
BstV1I GCAGC 7 cut(s) 141, 204, 232, 337, 385, 398, 488
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 48, 283
CaiI CAGNNNCTG 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 449
Csp6I GTAC 1 cut(s) 150
CviAII CATG 3 cut(s) 212, 262, 282
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 1 cut(s) 249
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 101
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 101
FaeI CATG 3 cut(s) 215, 265, 285
FatI CATG 3 cut(s) 211, 261, 281
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 226
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 7 cut(s) 155, 218, 221, 351, 387, 399, 477
FokI GGATG 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 7 cut(s) 155, 218, 221, 351, 387, 399, 477
FspBI CTAG 1 cut(s) 128
GluI GCNGC 7 cut(s) 155, 218, 221, 351, 387, 399, 477
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 1 cut(s) 449
Hin1II CATG 3 cut(s) 215, 265, 285
HinfI GANTC 2 cut(s) 425, 469
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 3 cut(s) 10, 133, 434
Hpy188I TCNGA 1 cut(s) 250
Hpy188III TCNNGA 2 cut(s) 63, 171
Hpy8I GTNNAC 3 cut(s) 10, 133, 434
HpyCH4III ACNGT 3 cut(s) 60, 91, 420
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 5 cut(s) 4, 70, 220, 386, 476
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 241, 383, 395
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 3 cut(s) 215, 265, 285
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 1 cut(s) 294
LpnPI CCDG 6 cut(s) 48, 156, 241, 414, 443, 485
Lsp1109I GCAGC 7 cut(s) 141, 204, 232, 337, 385, 398, 488
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 54, 207
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 2 cut(s) 212, 278
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 3 cut(s) 345, 355, 371
MnlI CCTC 3 cut(s) 174, 178, 490
MroXI GAANNNNTTC 2 cut(s) 137, 270
MseI TTAA 2 cut(s) 123, 333
Mva1269I GAATGC 1 cut(s) 72
MwoI GCNNNNNNNGC 4 cut(s) 160, 241, 383, 395
NdeI CATATG 1 cut(s) 226
NdeII GATC 1 cut(s) 294
NlaIII CATG 3 cut(s) 215, 265, 285
NspI RCATGY 1 cut(s) 285
PaeI GCATGC 1 cut(s) 285
PctI GAATGC 1 cut(s) 72
PdmI GAANNNNTTC 2 cut(s) 137, 270
PfeI GAWTC 2 cut(s) 425, 469
PkrI GCNGC 7 cut(s) 156, 219, 222, 352, 388, 400, 478
Psp124BI GAGCTC 1 cut(s) 101
PspPI GGNCC 1 cut(s) 48
PstI CTGCAG 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 2 cut(s) 123, 333
SatI GCNGC 7 cut(s) 155, 218, 221, 351, 387, 399, 477
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 101
SetI ASST 4 cut(s) 9, 101, 219, 237
SfcI CTRYAG 1 cut(s) 218
SpeI ACTAGT 1 cut(s) 127
SphI GCATGC 1 cut(s) 285
Sse9I AATT 3 cut(s) 345, 355, 371
SsiI CCGC 1 cut(s) 46
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 101
TaaI ACNGT 3 cut(s) 60, 91, 420
TaiI ACGT 1 cut(s) 9
TasI AATT 3 cut(s) 345, 355, 371
TfiI GAWTC 2 cut(s) 425, 469
Tru1I TTAA 2 cut(s) 123, 333
Tru9I TTAA 2 cut(s) 123, 333
TseI GCWGC 7 cut(s) 154, 217, 220, 350, 386, 398, 476
TspDTI ATGAA 2 cut(s) 465, 476
XceI RCATGY 1 cut(s) 285
XmnI GAANNNNTTC 2 cut(s) 137, 270
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.