Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0007946

Physical Location & Seq

Genomic Coordinates Forward (+)

73533227 .. 73542082

Gene Structure 8856 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00052790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGGGACCTTCAAAACACGATCGGAGTATCATCAGTGGACAGACGTACACGCGCCACCAGCGTCTCAACGACGCTCTTAACCTCGGCACCAGAGTGTGTGGCGGTGATAAGGCAAGGAGATGGCAGTGTATGGATATTGCGATACAGAGGCATGTGGTCCATTCTATTGCTGCGTTTCTTGATTCTGTTTCTGGGGATAAGCTGCACCATCCACTTTTGAAGGCTTCCGCTGCCGATATCATTGCAGCATTAGTCTGGATACTTCAGTCTAACAAGGGATCCATGTTGAGCACGGCAGCAAATGTGACAGTAAAATTAGTCAGCCTTTTACCCAAATCAATACTGCAGCTGGATGCTTTAGATCTTATTAATCCTTTGTCATCTTTGTTATCTGCCCATCAAACAGAAGTTTCTATATCATGTGCTGCAGCATTGAATCTTATCCTTACAAATCTCAGTATGAAGAATGCAAAAGAAGTTTGGGACATCCTTGAGAAAACAGAAAGCGTATCTCAAGTTATCAGTAACTTACGGTCTTTTTCAGAATGTATGATGCCATTTGAACATTTTCAACAGATGGCTTTACTTTTGAGTACAATACTATGGTGGTGGCCTCCATCTAGGTTTTTTGTTTGGAGTGATGCTAAACTGATGGAAGATTTAAATGATATGCTTATAAAGCGAGATGCTTATCACAAAGTTGCTGTTTTGAAATTGTATTCTGCCGTAGCTTTATGTGGTAATGGGGCCAAGAAAGTATTAGAAGGTGGAGAGGCACTTCTGGAACAGATGGTGCAATGCATGGACAGCTCATATCCTTATTCCGTTAGAATTGAAGCATTCAGGCTAGCACAGCATTTAGCGATAAATGAACAAAAATGCTTGCAAATGATGAGTTTATCTTGCCAACCTATTATTAAAGCCATAATTTCTGGAATGTGTGACTGGAGCTCACATTCTAGAAAGGTCACCAATGACCAATTGTCTTTGCTAGTGGAGGCATGTCGCTTGGCTCTGATCACTCGTTGGGCAGGGGAGCATCACATTTACTTCTGGAAACAAGGGATTGATAAAATCCTTCTCGATCTTCTCCATGAAAAATTTCACAACCAATCGTATGAAGACTCCATGTCATTGGAAGAGCAGATAGTTATTGCTAAGGAGGGTCTCAATGCCAATTATTTACTTTTTTTGAGGAGTTATGTCTGGGATATTCTTGGATGGCTTGCAATACATTGTGGGGAGGATTTCAAACCTGAAACTGAGTTCCACTTTGACATCCTTATTGCATGTGCATGCTTAACCTTTGTTGAGGCAATTCAGAAATGGCATAAAATATATGAAAACGACATTGCTCGTACCTTCAGAAGTGAATCGGCAACGAGGGCAGTTCTGATGATGATTTATTCTCCCTGCAAATACATTGCATCAAAGGCCAGATTTATATTGTCTGAAACACTGAAGCGAAAAGGCCTGGAGTATCTGAATATCTTACTGCGTTTTCTTAATAATCTGTCATCTGGGAATTTTGCGATACCAGATAAACTCCAAATTATCATTTATTTAATGGGATTTATATGCTATTCAGGTTTGGAAGAGTATCAAGTCTGGATTATCAAACAAAAAGGGGTAAAAACACTATTTTCTTTCGTGAAATGGTGCTTAAGTAATCATTTCCAAGTAGAAAGGTTGAGCTTTGCTCCCCATTTGCATAATACGTTTCATGAGAAAATTTGTTGTTCAAGTTCCAGAGAATGGGAAGGGAAAGACATTCTTTTGTTGTACAGTTTGTTGGGATTGGCTGAGTTGATAAAGCATTCTGGATGTGTAGGAGATAACCTAGACAGAATTGCCGGTAAGAATACATTGGCTCATATTGAAGCTGAACTAGTTAGTAAACTTCAGGACATCTGTAACAACAATTGTACCCCTGGGGTGCAATGGTATGCCGCATATGTTCTTAGTTATTTTGGGTTTTATGGTTTTCCGAGTAAGCTAATGAAAAGGATTGGTAAAGCAATTAATGATAAGGATTCTGCTGATTTACAATTCGTTCTCACAAATGGAGAGTGTTTCAGTGTTCATGGGGTGGTGCTTGCAATCCAATGTCCATCGCTACTGCCTCCTGAAGGGCTGCTTCCTAGTGAGGTAACATGTGATGATTCTTCAGTGACAGGCTCTATGGAGGTTTCTAAAGAGTTTCAGAAAGACATAAGATTATCTGCTCATGTCGATCACCAAGCATTGGTGATGTTGTTGGAGTATATCTACTTGGGATATGTACAAGCAGAAGAAGAACTTGCAAAAAAGTTGAGGACTCTTGCCAAACGTTGTAATTTACAGCCTCTGTTACAGATGCTTTGTAGAAAACGTCCAAAGTGGGGCATTGCTTTTCCTAGCCCTGATCTCAGTGCAGCTCTTGGTCCATCTGGACATCGTTTCTCGGATGTCATCTTAGAAGCGAAAGAAACAAAGCTTTTGGGCTGGACATGTGGTGTTTGCTCTTTATCAGTGCCTCACATGCATGTTCACAAGGTTGTATTGTCAGCCAGTTGTGATTATCTGAGGGCCTTGTTTGAGTCAGGAATGCAGGAGAGCAACTCACAAGCCATAAAGGTATCAATCAGTTGGAAAGCAATGGTTAAACTGGTCAACTACTTCTATTCTGGTAACCTGCCAAAGCCTCCTTCCGGATGTATATGGAATAACATGGACACTGAAGAAAAGTTAGATGAGGTACAGTCATATGTAGAGCTTTTTTGGCTCTCTGAGTTCTGGATCATGGAAGATGTCCAGGAAGCATGCTCGGATGCCATCATATCATGTCTTGGTTCTGCAAGAGAGTTGGCTCTTAAAATACTACAAATTGCTGCGAATTTATCTCTCTGGAACTTGGCCGAGGTTGCAGCGTCTTATATGGCCCCTTTATATCGCCAGTTATGTGATTCTGGTGAACTTGAAACACTTGATGAAATGCTGGCTGATATGGTTCGGGTAGCGTCTGTTCGATTCTCCCAACAGGGTGGTGATCAGTTTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB

Protein Analysis

1013

Amino Acids

114.26

Weight (kDa)

6.29

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_6	PF26522	133 - 246	2.1e-47	Plant ARM repeat
BTB	PF00651	840 - 910	1.6e-09	BTB/POZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007946)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04390
fragaria_vesca	FvH4_3g15860
malus_domestica	MD05G1219300.v1.1
prunus_persica	Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1 Prupe.4G140600_v2.0.a1
pyrus_communis	pycom05g20050 pycom05g20060
rosa_chinensis	RchiOBHm_Chr5g0026671
rosa_laevigata	RLG00000032945
rosa_multiflora	Rmu_co8501061.1_g000001 Rmu_sc0004490.1_g000009
rosa_roxburghii	Rroxscaffold_1G00052790
rosa_rugosa	Rorug05G0094700 Rorug05G0094700
rosa_samantha	Rh5AG188300 Rh5BG184600 Rh5CG204500 Rh5DG186900
rosa_wichuraiana	Rw5G017040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	677
Acc36I	ACCTGC	1 cut(s)	2682
AccB1I	GGYRCC	1 cut(s)	86
AccB7I	CCANNNNNTGG	2 cut(s)	1755, 2245
AccII	CGCG	1 cut(s)	52
AccIII	TCCGGA	1 cut(s)	2690
AciI	CCGC	3 cut(s)	102, 228, 1950
AclI	AACGTT	1 cut(s)	2329
AclWI	GGATC	3 cut(s)	273, 286, 2786
AcoI	YGGCCR	1 cut(s)	2895
AcsI	RAATTY	4 cut(s)	1100, 1525, 1731, 2875
AcuI	CTGAAG	7 cut(s)	248, 1348, 1481, 1886, 2148, 2151, 2739
AfaI	GTAC	7 cut(s)	47, 595, 1360, 1784, 1927, 2283, 2739
AfiI	CCNNNNNNNGG	5 cut(s)	1755, 2129, 2245, 2381, 2690
AflII	CTTAAG	1 cut(s)	1663
AflIII	ACRYGT	2 cut(s)	2153, 2489
AhdI	GACNNNNNGTC	2 cut(s)	982, 1129
AhlI	ACTAGT	1 cut(s)	1888
AjnI	CCWGG	3 cut(s)	1473, 1930, 2793
AjuI	GAANNNNNNNTTGG	2 cut(s)	2671, 2703
AleI	CACNNNNGTG	1 cut(s)	92
Alw21I	GWGCWC	2 cut(s)	293, 953
Alw26I	GTCTC	2 cut(s)	68, 1172
AlwI	GGATC	3 cut(s)	273, 286, 2786
AlwNI	CAGNNNCTG	1 cut(s)	2347
Aor13HI	TCCGGA	1 cut(s)	2690
AoxI	GGCC	7 cut(s)	611, 747, 1434, 1471, 2568, 2895, 2919
ApoI	RAATTY	4 cut(s)	1100, 1525, 1731, 2875
AseI	ATTAAT	2 cut(s)	369, 2022
Asp700I	GAANNNNTTC	2 cut(s)	567, 1101
AspLEI	GCGC	1 cut(s)	54
AspS9I	GGNCC	6 cut(s)	5, 157, 747, 2423, 2568, 2920
AsuHPI	GGTGA	6 cut(s)	116, 961, 2228, 2260, 2963, 3038
AsuNHI	GCTAGC	1 cut(s)	847
AvaII	GGWCC	3 cut(s)	5, 157, 2423
BaeI	ACNNNNGTAYC	4 cut(s)	251, 284, 1909, 1942
BamHI	GGATCC	1 cut(s)	278
BanI	GGYRCC	1 cut(s)	86
BanII	GRGCYC	1 cut(s)	953
BbsI	GAAGAC	1 cut(s)	1128
Bbv12I	GWGCWC	2 cut(s)	293, 953
BceAI	ACGGC	2 cut(s)	309, 710
BcgI	CGANNNNNNTGC	2 cut(s)	224, 258
BciT130I	CCWGG	3 cut(s)	1475, 1932, 2795
BciVI	GTATCC	1 cut(s)	252
BclI	TGATCA	2 cut(s)	1017, 3028
BcoDI	GTCTC	2 cut(s)	68, 1172
BcuI	ACTAGT	1 cut(s)	1888
BfaI	CTAG	8 cut(s)	621, 848, 960, 992, 1841, 1889, 2142, 2397
BfmI	CTRYAG	2 cut(s)	344, 426
BfrI	CTTAAG	1 cut(s)	1663
BfuAI	ACCTGC	1 cut(s)	2682
BfuI	GTATCC	1 cut(s)	252
BglII	AGATCT	1 cut(s)	361
Bme1390I	CCNGG	3 cut(s)	1475, 1932, 2795
Bme18I	GGWCC	3 cut(s)	5, 157, 2423
BmeRI	GACNNNNNGTC	2 cut(s)	982, 1129
BmgT120I	GGNCC	6 cut(s)	5, 157, 747, 2423, 2568, 2920
BmiI	GGNNCC	5 cut(s)	6, 88, 280, 748, 2922
BmrFI	CCNGG	3 cut(s)	1475, 1932, 2795
BmsI	GCATC	8 cut(s)	343, 543, 631, 676, 1048, 1436, 2346, 2800
BmtI	GCTAGC	1 cut(s)	851
BpiI	GAAGAC	1 cut(s)	1128
BplI	GAGNNNNNCTC	4 cut(s)	1684, 1716, 2585, 2617
BpmI	CTGGAG	2 cut(s)	967, 1496
Bpu10I	CCTNAGC	1 cut(s)	1158
BpuEI	CTTGAG	2 cut(s)	498, 512
BsaBI	GATNNNNATC	1 cut(s)	690
BsaI	GGTCTC	1 cut(s)	1172
BsaJI	CCNNGG	4 cut(s)	82, 1930, 1931, 2898
BsaWI	WCCGGW	1 cut(s)	2690
Bsc4I	CCNNNNNNNGG	5 cut(s)	1755, 2129, 2245, 2381, 2690
Bse118I	RCCGGY	1 cut(s)	1853
Bse1I	ACTGG	4 cut(s)	950, 2550, 2652, 2935
Bse3DI	GCAATG	7 cut(s)	240, 803, 1350, 1422, 1946, 2385, 2643
Bse8I	GATNNNNATC	1 cut(s)	690
BseAI	TCCGGA	1 cut(s)	2690
BseBI	CCWGG	3 cut(s)	1475, 1932, 2795
BseDI	CCNNGG	4 cut(s)	82, 1930, 1931, 2898
BseGI	GGATG	9 cut(s)	208, 358, 486, 1226, 1278, 1829, 2452, 2699, 2815
BseJI	GATNNNNATC	1 cut(s)	690
BseLI	CCNNNNNNNGG	5 cut(s)	1755, 2129, 2245, 2381, 2690
BseMI	GCAATG	7 cut(s)	240, 803, 1350, 1422, 1946, 2385, 2643
BseMII	CTCAG	6 cut(s)	469, 1254, 1794, 2422, 2555, 2760
BseNI	ACTGG	4 cut(s)	950, 2550, 2652, 2935
BseRI	GAGGAG	1 cut(s)	1210
BsgI	GTGCAG	2 cut(s)	188, 2433
Bsh1236I	CGCG	1 cut(s)	52
Bsh1285I	CGRYCG	1 cut(s)	22
BshFI	GGCC	7 cut(s)	613, 749, 1436, 1473, 2570, 2897, 2921
BshNI	GGYRCC	1 cut(s)	86
BsiEI	CGRYCG	1 cut(s)	22
BsiHKAI	GWGCWC	2 cut(s)	293, 953
BsiSI	CCGG	2 cut(s)	1854, 2691
BslFI	GGGAC	2 cut(s)	18, 497
BslI	CCNNNNNNNGG	5 cut(s)	1755, 2129, 2245, 2381, 2690
BsmAI	GTCTC	2 cut(s)	68, 1172
BsmBI	CGTCTC	1 cut(s)	68
BsmFI	GGGAC	2 cut(s)	18, 497
BsmI	GAATGC	4 cut(s)	472, 839, 1816, 2592
BsnI	GGCC	7 cut(s)	613, 749, 1436, 1473, 2570, 2897, 2921
Bso31I	GGTCTC	1 cut(s)	1172
Bsp1286I	GDGCHC	2 cut(s)	293, 953
Bsp13I	TCCGGA	1 cut(s)	2690
Bsp1407I	TGTACA	2 cut(s)	1782, 2281
Bsp143I	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
BspACI	CCGC	3 cut(s)	102, 228, 1950
BspANI	GGCC	7 cut(s)	613, 749, 1436, 1473, 2570, 2897, 2921
BspCNI	CTCAG	6 cut(s)	468, 1255, 1795, 2421, 2556, 2761
BspEI	TCCGGA	1 cut(s)	2690
BspFNI	CGCG	1 cut(s)	52
BspHI	TCATGA	1 cut(s)	1723
BspLI	GGNNCC	5 cut(s)	6, 88, 280, 748, 2922
BspMAI	CTGCAG	2 cut(s)	348, 430
BspMI	ACCTGC	1 cut(s)	2682
BspOI	GCTAGC	1 cut(s)	851
BspPI	GGATC	3 cut(s)	273, 286, 2786
BspQI	GCTCTTC	1 cut(s)	1134
BspT107I	GGYRCC	1 cut(s)	86
BspTI	CTTAAG	1 cut(s)	1663
BspTNI	GGTCTC	1 cut(s)	1172
BsrDI	GCAATG	7 cut(s)	240, 803, 1350, 1422, 1946, 2385, 2643
BsrFI	RCCGGY	1 cut(s)	1853
BsrGI	TGTACA	2 cut(s)	1782, 2281
BsrI	ACTGG	4 cut(s)	950, 2550, 2652, 2935
BssAI	RCCGGY	1 cut(s)	1853
BssECI	CCNNGG	4 cut(s)	82, 1930, 1931, 2898
BssMI	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
Bst2UI	CCWGG	3 cut(s)	1475, 1932, 2795
Bst4CI	ACNGT	4 cut(s)	310, 534, 1787, 2742
Bst6I	CTCTTC	2 cut(s)	1134, 1590
BstAFI	CTTAAG	1 cut(s)	1663
BstAUI	TGTACA	2 cut(s)	1782, 2281
BstC8I	GCNNGC	7 cut(s)	849, 884, 1227, 1297, 2097, 2803, 2979
BstDEI	CTNAG	9 cut(s)	455, 1158, 1263, 1803, 1961, 2408, 2455, 2564, 2769
BstEII	GGTNACC	2 cut(s)	967, 2669
BstENI	CCTNNNNNAGG	1 cut(s)	2127
BstF5I	GGATG	9 cut(s)	208, 358, 486, 1226, 1278, 1829, 2452, 2699, 2815
BstFNI	CGCG	1 cut(s)	52
BstHHI	GCGC	1 cut(s)	54
BstKTI	GATC	9 cut(s)	22, 281, 364, 1020, 1087, 2236, 2407, 2781, 3031
BstMAI	GTCTC	2 cut(s)	68, 1172
BstMBI	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
BstMCI	CGRYCG	1 cut(s)	22
BstNI	CCWGG	3 cut(s)	1475, 1932, 2795
BstNSI	RCATGY	9 cut(s)	155, 1005, 1293, 1299, 2157, 2493, 2524, 2528, 2805
BstPI	GGTNACC	2 cut(s)	967, 2669
BstSCI	CCNGG	3 cut(s)	1473, 1930, 2793
BstSFI	CTRYAG	2 cut(s)	344, 426
BstUI	CGCG	1 cut(s)	52
BstV2I	GAAGAC	1 cut(s)	1128
BstX2I	RGATCY	2 cut(s)	278, 361
BstXI	CCANNNNNNTGG	2 cut(s)	1135, 3023
BstYI	RGATCY	2 cut(s)	278, 361
BsuI	GTATCC	1 cut(s)	252
BsuRI	GGCC	7 cut(s)	613, 749, 1436, 1473, 2570, 2897, 2921
BtgZI	GCGATG	1 cut(s)	2097
BtsCI	GGATG	9 cut(s)	208, 358, 486, 1226, 1278, 1829, 2452, 2699, 2815
BtsI	GCAGTG	1 cut(s)	131
BtsIMutI	CAGTG	8 cut(s)	40, 131, 1457, 2083, 2175, 2416, 2517, 2715
BveI	ACCTGC	1 cut(s)	2682
Cac8I	GCNNGC	7 cut(s)	849, 884, 1227, 1297, 2097, 2803, 2979
CaiI	CAGNNNCTG	1 cut(s)	2347
CciI	TCATGA	1 cut(s)	1723
CfoI	GCGC	1 cut(s)	54
Cfr10I	RCCGGY	1 cut(s)	1853
Cfr13I	GGNCC	6 cut(s)	5, 157, 747, 2423, 2568, 2920
CseI	GACGC	4 cut(s)	50, 80, 2898, 2988
Csp6I	GTAC	7 cut(s)	46, 594, 1359, 1783, 1926, 2282, 2738
CviQI	GTAC	7 cut(s)	46, 594, 1359, 1783, 1926, 2282, 2738
DdeI	CTNAG	9 cut(s)	455, 1158, 1263, 1803, 1961, 2408, 2455, 2564, 2769
DpnI	GATC	9 cut(s)	21, 280, 363, 1019, 1086, 2235, 2406, 2780, 3030
DpnII	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
DraI	TTTAAA	1 cut(s)	663
DriI	GACNNNNNGTC	2 cut(s)	982, 1129
EaeI	YGGCCR	1 cut(s)	2895
Eam1104I	CTCTTC	2 cut(s)	1134, 1590
Eam1105I	GACNNNNNGTC	2 cut(s)	982, 1129
EarI	CTCTTC	2 cut(s)	1134, 1590
Ecl136II	GAGCTC	1 cut(s)	951
Eco147I	AGGCCT	1 cut(s)	1473
Eco24I	GRGCYC	1 cut(s)	953
Eco31I	GGTCTC	1 cut(s)	1172
Eco32I	GATATC	1 cut(s)	238
Eco47I	GGWCC	3 cut(s)	5, 157, 2423
Eco53kI	GAGCTC	1 cut(s)	951
Eco57I	CTGAAG	7 cut(s)	248, 1348, 1481, 1886, 2148, 2151, 2739
Eco91I	GGTNACC	2 cut(s)	967, 2669
EcoICRI	GAGCTC	1 cut(s)	951
EcoNI	CCTNNNNNAGG	1 cut(s)	2127
EcoO109I	RGGNCCY	2 cut(s)	5, 2568
EcoO65I	GGTNACC	2 cut(s)	967, 2669
EcoRII	CCWGG	3 cut(s)	1473, 1930, 2793
EcoRV	GATATC	1 cut(s)	238
EcoT22I	ATGCAT	2 cut(s)	803, 2526
EcoT38I	GRGCYC	1 cut(s)	953
Esp3I	CGTCTC	1 cut(s)	68
FalI	AAGNNNNNCTT	6 cut(s)	1758, 1790, 2121, 2153, 2283, 2315
FaqI	GGGAC	2 cut(s)	18, 497
FauNDI	CATATG	2 cut(s)	1954, 2746
FbaI	TGATCA	2 cut(s)	1017, 3028
FokI	GGATG	9 cut(s)	195, 365, 473, 1233, 1265, 1836, 2459, 2706, 2822
FriOI	GRGCYC	1 cut(s)	953
FspBI	CTAG	8 cut(s)	621, 848, 960, 992, 1841, 1889, 2142, 2397
GlaI	GCGC	1 cut(s)	53
GsuI	CTGGAG	2 cut(s)	967, 1496
HaeIII	GGCC	7 cut(s)	613, 749, 1436, 1473, 2570, 2897, 2921
HapII	CCGG	2 cut(s)	1854, 2691
HgaI	GACGC	4 cut(s)	50, 80, 2898, 2988
HhaI	GCGC	1 cut(s)	54
Hin6I	GCGC	1 cut(s)	52
HinP1I	GCGC	1 cut(s)	52
HincII	GTYRAC	1 cut(s)	2653
HindII	GTYRAC	1 cut(s)	2653
HindIII	AAGCTT	1 cut(s)	2474
HpaII	CCGG	2 cut(s)	1854, 2691
HphI	GGTGA	6 cut(s)	116, 961, 2228, 2260, 2963, 3038
Hpy166II	GTNNAC	6 cut(s)	38, 48, 1898, 2530, 2653, 2954
Hpy8I	GTNNAC	6 cut(s)	38, 48, 1898, 2530, 2653, 2954
Hpy99I	CGWCG	1 cut(s)	74
HpyAV	CCTTC	8 cut(s)	18, 214, 758, 1088, 1372, 1754, 2123, 2697
HpyCH4III	ACNGT	4 cut(s)	310, 534, 1787, 2742
HpyCH4IV	ACGT	4 cut(s)	44, 1718, 2329, 2371
HpyF3I	CTNAG	9 cut(s)	455, 1158, 1263, 1803, 1961, 2408, 2455, 2564, 2769
HpySE526I	ACGT	4 cut(s)	44, 1718, 2329, 2371
HspAI	GCGC	1 cut(s)	52
Kpn2I	TCCGGA	1 cut(s)	2690
Ksp22I	TGATCA	2 cut(s)	1017, 3028
Kzo9I	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
LguI	GCTCTTC	1 cut(s)	1134
LmnI	GCTCC	3 cut(s)	948, 1036, 1705
LweI	GCATC	8 cut(s)	343, 543, 631, 676, 1048, 1436, 2346, 2800
MaeI	CTAG	8 cut(s)	621, 848, 960, 992, 1841, 1889, 2142, 2397
MaeII	ACGT	4 cut(s)	44, 1718, 2329, 2371
MaeIII	GTNAC	9 cut(s)	304, 524, 941, 967, 1913, 2149, 2170, 2349, 2669
MalI	GATC	9 cut(s)	21, 280, 363, 1019, 1086, 2235, 2406, 2780, 3030
MboI	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
MfeI	CAATTG	2 cut(s)	980, 1921
MflI	RGATCY	2 cut(s)	278, 361
MhlI	GDGCHC	2 cut(s)	293, 953
MlyI	GAGTC	3 cut(s)	1118, 2311, 2588
MmeI	TCCRAC	2 cut(s)	2238, 2609
Mph1103I	ATGCAT	2 cut(s)	803, 2526
MroI	TCCGGA	1 cut(s)	2690
MroXI	GAANNNNTTC	2 cut(s)	567, 1101
MslI	CAYNNNNRTG	3 cut(s)	92, 1294, 2523
MspA1I	CMGCKG	2 cut(s)	230, 349
MspCI	CTTAAG	1 cut(s)	1663
MspI	CCGG	2 cut(s)	1854, 2691
MspR9I	CCNGG	3 cut(s)	1475, 1932, 2795
MunI	CAATTG	2 cut(s)	980, 1921
Mva1269I	GAATGC	4 cut(s)	472, 839, 1816, 2592
MvaI	CCWGG	3 cut(s)	1475, 1932, 2795
MvnI	CGCG	1 cut(s)	52
NdeI	CATATG	2 cut(s)	1954, 2746
NdeII	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
NheI	GCTAGC	1 cut(s)	847
NlaIV	GGNNCC	5 cut(s)	6, 88, 280, 748, 2922
NmeAIII	GCCGAG	2 cut(s)	63, 2923
NmuCI	GTSAC	4 cut(s)	304, 941, 967, 2170
NsiI	ATGCAT	2 cut(s)	803, 2526
NspI	RCATGY	9 cut(s)	155, 1005, 1293, 1299, 2157, 2493, 2524, 2528, 2805
OliI	CACNNNNGTG	1 cut(s)	92
PaeI	GCATGC	2 cut(s)	1299, 2805
PagI	TCATGA	1 cut(s)	1723
PasI	CCCWGGG	1 cut(s)	1931
PceI	AGGCCT	1 cut(s)	1473
PciI	ACATGT	2 cut(s)	2153, 2489
PciSI	GCTCTTC	1 cut(s)	1134
PctI	GAATGC	4 cut(s)	472, 839, 1816, 2592
PdmI	GAANNNNTTC	2 cut(s)	567, 1101
PfeI	GAWTC	7 cut(s)	182, 436, 1373, 2033, 2162, 2945, 3009
PflMI	CCANNNNNTGG	2 cut(s)	1755, 2245
PfoI	TCCNGGA	1 cut(s)	2793
Ple19I	CGATCG	1 cut(s)	22
PleI	GAGTC	3 cut(s)	1118, 2311, 2587
PpsI	GAGTC	3 cut(s)	1118, 2311, 2587
PpuMI	RGGWCCY	1 cut(s)	5
PscI	ACATGT	2 cut(s)	2153, 2489
PshBI	ATTAAT	2 cut(s)	369, 2022
PsiI	TTATAA	1 cut(s)	677
Psp124BI	GAGCTC	1 cut(s)	953
Psp1406I	AACGTT	1 cut(s)	2329
Psp5II	RGGWCCY	1 cut(s)	5
Psp6I	CCWGG	3 cut(s)	1473, 1930, 2793
PspEI	GGTNACC	2 cut(s)	967, 2669
PspGI	CCWGG	3 cut(s)	1473, 1930, 2793
PspN4I	GGNNCC	5 cut(s)	6, 88, 280, 748, 2922
PspPI	GGNCC	6 cut(s)	5, 157, 747, 2423, 2568, 2920
PspPPI	RGGWCCY	1 cut(s)	5
PsrI	GAACNNNNNNTAC	2 cut(s)	2988, 3020
PstI	CTGCAG	2 cut(s)	348, 430
PstNI	CAGNNNCTG	1 cut(s)	2347
PsuI	RGATCY	2 cut(s)	278, 361
PvuI	CGATCG	1 cut(s)	22
PvuII	CAGCTG	1 cut(s)	349
RsaI	GTAC	7 cut(s)	47, 595, 1360, 1784, 1927, 2283, 2739
RsaNI	GTAC	7 cut(s)	46, 594, 1359, 1783, 1926, 2282, 2738
RseI	CAYNNNNRTG	3 cut(s)	92, 1294, 2523
SacI	GAGCTC	1 cut(s)	953
SapI	GCTCTTC	1 cut(s)	1134
Sau3AI	GATC	9 cut(s)	19, 278, 361, 1017, 1084, 2233, 2404, 2778, 3028
Sau96I	GGNCC	6 cut(s)	5, 157, 747, 2423, 2568, 2920
SchI	GAGTC	3 cut(s)	1118, 2311, 2588
ScrFI	CCNGG	3 cut(s)	1475, 1932, 2795
SduI	GDGCHC	2 cut(s)	293, 953
SfaNI	GCATC	8 cut(s)	343, 543, 631, 676, 1048, 1436, 2346, 2800
SfcI	CTRYAG	2 cut(s)	344, 426
SinI	GGWCC	3 cut(s)	5, 157, 2423
SmiI	ATTTAAAT	1 cut(s)	663
SmiMI	CAYNNNNRTG	3 cut(s)	92, 1294, 2523
SmlI	CTYRAG	3 cut(s)	491, 513, 1663
SmoI	CTYRAG	3 cut(s)	491, 513, 1663
SpeI	ACTAGT	1 cut(s)	1888
SphI	GCATGC	2 cut(s)	1299, 2805
SseBI	AGGCCT	1 cut(s)	1473
SsiI	CCGC	3 cut(s)	102, 228, 1950
SspMI	CTAG	8 cut(s)	621, 848, 960, 992, 1841, 1889, 2142, 2397
SstI	GAGCTC	1 cut(s)	953
StuI	AGGCCT	1 cut(s)	1473
StyD4I	CCNGG	3 cut(s)	1473, 1930, 2793
SwaI	ATTTAAAT	1 cut(s)	663
TaaI	ACNGT	4 cut(s)	310, 534, 1787, 2742
TaiI	ACGT	4 cut(s)	47, 1721, 2332, 2374
TaqI	TCGA	3 cut(s)	1083, 2232, 3007
TatI	WGTACW	3 cut(s)	593, 1782, 2281
TauI	GCSGC	1 cut(s)	1952
TfiI	GAWTC	7 cut(s)	182, 436, 1373, 2033, 2162, 2945, 3009
TscAI	CASTG	8 cut(s)	40, 131, 1464, 2083, 2175, 2416, 2517, 2722
TseFI	GTSAC	4 cut(s)	304, 941, 967, 2170
Tsp45I	GTSAC	4 cut(s)	304, 941, 967, 2170
TspDTI	ATGAA	9 cut(s)	476, 885, 1110, 1134, 1356, 1712, 2015, 2072, 2985
TspGWI	ACGGA	1 cut(s)	814
TspRI	CASTG	8 cut(s)	40, 131, 1464, 2083, 2175, 2416, 2517, 2722
Van91I	CCANNNNNTGG	2 cut(s)	1755, 2245
Vha464I	CTTAAG	1 cut(s)	1663
VpaK11BI	GGWCC	3 cut(s)	5, 157, 2423
VspI	ATTAAT	2 cut(s)	369, 2022
XagI	CCTNNNNNAGG	1 cut(s)	2127
XapI	RAATTY	4 cut(s)	1100, 1525, 1731, 2875
XbaI	TCTAGA	1 cut(s)	959
XceI	RCATGY	9 cut(s)	155, 1005, 1293, 1299, 2157, 2493, 2524, 2528, 2805
XmnI	GAANNNNTTC	2 cut(s)	567, 1101
XspI	CTAG	8 cut(s)	621, 848, 960, 992, 1841, 1889, 2142, 2397
Zsp2I	ATGCAT	2 cut(s)	803, 2526

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.