Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009056

Physical Location & Seq

Genomic Coordinates Forward (+)

36986104 .. 36988333

Gene Structure 2230 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1238800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 834 bp

ATGGAACTAATTGATGCTGCCTTTCCTCTTCTCAAAGGTGTTGTTGCTACAACAGATGCTCAGGAAGCTTGTAAAGATGTCAATATTGCTGTTATGGTGGGCGGATTCCCGCGGAAGGAAGGTATGGAAAGAAAGGATGTGATGTCGAAAAATGTATCAATCTACAAGGCTCAAGCTTCAGCACTAGAAAAGCATGCTGCTTCAGATGTCAAGGTTCTAGTGGTTGCGAACCCAGCAAACACTAATGCACTCATCTTGAAAGAATATGCACCTAAAATCCCAGAGAAGAACATCACATGTCATACAAGACTAGATCATAACAGAGCTTTGGGACAAATCTCCGAGAAGCTAAATGTTCATGTTGGGGATGTAAAGAATGTTATCATCTGGGGAAATCACTCTTCAACTCAGTATCCTGATGTCAATCATGCAACTGTCACCACTAGCAGCGGAGAGAAACCTGTCAGGGAACTTGTTGCTAATGATCAATGGTTAAATACCGAGTTCATCACCACTGTGCAGCAGCGCGGTGCAGCCATTATTAAGGCTCGGAAGCTATCAAGTGCATTGTCTGCTGCCAGTTCTGCTTGTGATCACATACGTGATTGGGTTCTTGGGACTCCTAAGGGGACATGGGTTTCAATGGGTGTGTACTCTGATGGTTCTTATGGAATCCAGCAAGGCCTAATCTACTCCTTCCCTGTTACATGTGATAAAGGACAATGGTCAATTGTGCAGGGACTCAAGATTGACGATTTCTCAAGGGGAAAGCTAGATGCAACTGCAAAAGAGCTCATCGAGGAGAAAACATTGGCCTATTCATGTCTCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009987 GO:0015980 GO:0016020 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0034641 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04964 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200 map04964

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

278

Amino Acids

30.17

Weight (kDa)

7.65

Isoelectric Point (pI)

28.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	1 - 98	1.3e-21	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	102 - 270	9.3e-47	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56720
fragaria_vesca	FvH4_3g14020 FvH4_3g14020 FvH4_3g14020
malus_domestica	MD05G1238800.v1.1 MD10G1216900.v1.1
prunus_persica	Prupe.4G127500_v2.0.a1 Prupe.4G127500_v2.0.a1
pyrus_communis	pycom05g21480 pycom10g18290
rosa_chinensis	RchiOBHm_Chr5g0023331
rosa_laevigata	RLG00000032714
rosa_multiflora	Rmu_sc0004693.1_g000004 Rmu_sc0009954.1_g000015
rosa_roxburghii	Rroxscaffold_1G00056000
rosa_rugosa	Rorug05G0078400 Rorug05G0078500
rosa_samantha	Rh5AG165000 Rh5BG164500 Rh5CG179400 Rh5DG166000
rosa_wichuraiana	Rw5G014870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	112, 528
AciI	CCGC	5 cut(s)	102, 110, 112, 450, 528
AcuI	CTGAAG	2 cut(s)	162, 186
AfaI	GTAC	1 cut(s)	653
AfiI	CCNNNNNNNGG	1 cut(s)	115
AflIII	ACRYGT	2 cut(s)	296, 707
AgsI	TTSAA	3 cut(s)	259, 405, 642
AleI	CACNNNNGTG	2 cut(s)	515, 600
AluBI	AGCT	7 cut(s)	68, 176, 326, 349, 556, 772, 793
AluI	AGCT	7 cut(s)	68, 176, 326, 349, 556, 772, 793
Alw21I	GWGCWC	1 cut(s)	795
Alw26I	GTCTC	1 cut(s)	830
AoxI	GGCC	2 cut(s)	682, 813
ApeKI	GCWGC	7 cut(s)	17, 197, 447, 520, 523, 533, 575
AspLEI	GCGC	1 cut(s)	528
AsuHPI	GGTGA	2 cut(s)	430, 502
AxyI	CCTNAGG	1 cut(s)	624
BanII	GRGCYC	1 cut(s)	795
Bbv12I	GWGCWC	1 cut(s)	795
BbvI	GCAGC	7 cut(s)	4, 184, 459, 532, 535, 545, 562
BccI	CCATC	1 cut(s)	653
BciVI	GTATCC	1 cut(s)	423
BclI	TGATCA	2 cut(s)	484, 592
BcoDI	GTCTC	1 cut(s)	830
BfaI	CTAG	5 cut(s)	185, 218, 311, 444, 773
BfuI	GTATCC	1 cut(s)	423
BisI	GCNGC	7 cut(s)	18, 198, 448, 521, 524, 534, 576
BlsI	GCNGC	7 cut(s)	19, 199, 449, 522, 525, 535, 577
BmsI	GCATC	3 cut(s)	4, 46, 766
BoxI	GACNNNNGTC	1 cut(s)	724
Bpu10I	CCTNAGC	1 cut(s)	60
BpuEI	CTTGAG	3 cut(s)	156, 728, 745
BsaAI	YACGTR	1 cut(s)	602
BsaBI	GATNNNNATC	1 cut(s)	423
BsaJI	CCNNGG	1 cut(s)	110
Bsc4I	CCNNNNNNNGG	1 cut(s)	115
Bse1I	ACTGG	1 cut(s)	579
Bse21I	CCTNAGG	1 cut(s)	624
Bse8I	GATNNNNATC	1 cut(s)	423
BseDI	CCNNGG	1 cut(s)	110
BseGI	GGATG	2 cut(s)	142, 373
BseJI	GATNNNNATC	1 cut(s)	423
BseLI	CCNNNNNNNGG	1 cut(s)	115
BseMII	CTCAG	2 cut(s)	74, 422
BseNI	ACTGG	1 cut(s)	579
BseRI	GAGGAG	1 cut(s)	815
BseXI	GCAGC	7 cut(s)	4, 184, 459, 532, 535, 545, 562
BseYI	CCCAGC	1 cut(s)	232
BsgI	GTGCAG	3 cut(s)	539, 552, 755
Bsh1236I	CGCG	2 cut(s)	112, 528
BshFI	GGCC	2 cut(s)	684, 815
BsiHKAI	GWGCWC	1 cut(s)	795
BslFI	GGGAC	4 cut(s)	345, 631, 643, 753
BslI	CCNNNNNNNGG	1 cut(s)	115
BsmAI	GTCTC	1 cut(s)	830
BsmFI	GGGAC	4 cut(s)	345, 631, 643, 753
BsnI	GGCC	2 cut(s)	684, 815
Bsp1286I	GDGCHC	1 cut(s)	795
Bsp143I	GATC	3 cut(s)	313, 484, 592
BspACI	CCGC	5 cut(s)	102, 110, 112, 450, 528
BspANI	GGCC	2 cut(s)	684, 815
BspCNI	CTCAG	2 cut(s)	73, 421
BspFNI	CGCG	2 cut(s)	112, 528
BsrI	ACTGG	1 cut(s)	579
BssECI	CCNNGG	1 cut(s)	110
BssMI	GATC	3 cut(s)	313, 484, 592
Bst4CI	ACNGT	2 cut(s)	436, 517
Bst6I	CTCTTC	2 cut(s)	33, 406
BstAPI	GCANNNNNTGC	1 cut(s)	572
BstBAI	YACGTR	1 cut(s)	602
BstC8I	GCNNGC	1 cut(s)	195
BstDEI	CTNAG	3 cut(s)	60, 408, 624
BstDSI	CCRYGG	1 cut(s)	110
BstF5I	GGATG	2 cut(s)	142, 373
BstFNI	CGCG	2 cut(s)	112, 528
BstHHI	GCGC	1 cut(s)	528
BstKTI	GATC	3 cut(s)	316, 487, 595
BstMAI	GTCTC	1 cut(s)	830
BstMBI	GATC	3 cut(s)	313, 484, 592
BstMWI	GCNNNNNNNGC	4 cut(s)	65, 233, 572, 584
BstNSI	RCATGY	3 cut(s)	197, 300, 711
BstPAI	GACNNNNGTC	1 cut(s)	724
BstUI	CGCG	2 cut(s)	112, 528
BstV1I	GCAGC	7 cut(s)	4, 184, 459, 532, 535, 545, 562
Bsu36I	CCTNAGG	1 cut(s)	624
BsuI	GTATCC	1 cut(s)	423
BsuRI	GGCC	2 cut(s)	684, 815
BtgI	CCRYGG	1 cut(s)	110
BtsCI	GGATG	2 cut(s)	142, 373
BtsIMutI	CAGTG	1 cut(s)	513
Cac8I	GCNNGC	1 cut(s)	195
CfoI	GCGC	1 cut(s)	528
Cfr42I	CCGCGG	1 cut(s)	113
Csp6I	GTAC	1 cut(s)	652
CviAII	CATG	7 cut(s)	194, 297, 359, 428, 633, 708, 822
CviQI	GTAC	1 cut(s)	652
DdeI	CTNAG	3 cut(s)	60, 408, 624
DpnI	GATC	3 cut(s)	315, 486, 594
DpnII	GATC	3 cut(s)	313, 484, 592
Eam1104I	CTCTTC	2 cut(s)	33, 406
EarI	CTCTTC	2 cut(s)	33, 406
EciI	GGCGGA	1 cut(s)	117
Ecl136II	GAGCTC	1 cut(s)	793
Eco147I	AGGCCT	1 cut(s)	684
Eco24I	GRGCYC	1 cut(s)	795
Eco53kI	GAGCTC	1 cut(s)	793
Eco57I	CTGAAG	2 cut(s)	162, 186
Eco81I	CCTNAGG	1 cut(s)	624
EcoICRI	GAGCTC	1 cut(s)	793
EcoT38I	GRGCYC	1 cut(s)	795
FaeI	CATG	7 cut(s)	197, 300, 362, 431, 636, 711, 825
FaqI	GGGAC	4 cut(s)	345, 631, 643, 753
FatI	CATG	7 cut(s)	193, 296, 358, 427, 632, 707, 821
FauI	CCCGC	1 cut(s)	117
FbaI	TGATCA	2 cut(s)	484, 592
Fnu4HI	GCNGC	7 cut(s)	18, 198, 448, 521, 524, 534, 576
FokI	GGATG	2 cut(s)	149, 380
FriOI	GRGCYC	1 cut(s)	795
Fsp4HI	GCNGC	7 cut(s)	18, 198, 448, 521, 524, 534, 576
FspBI	CTAG	5 cut(s)	185, 218, 311, 444, 773
GlaI	GCGC	1 cut(s)	527
GluI	GCNGC	7 cut(s)	18, 198, 448, 521, 524, 534, 576
GsaI	CCCAGC	1 cut(s)	236
HaeIII	GGCC	2 cut(s)	684, 815
HhaI	GCGC	1 cut(s)	528
Hin1II	CATG	7 cut(s)	197, 300, 362, 431, 636, 711, 825
Hin6I	GCGC	1 cut(s)	526
HinP1I	GCGC	1 cut(s)	526
HindIII	AAGCTT	2 cut(s)	66, 174
HinfI	GANTC	4 cut(s)	105, 619, 672, 741
HphI	GGTGA	2 cut(s)	430, 502
Hpy166II	GTNNAC	1 cut(s)	652
Hpy188I	TCNGA	4 cut(s)	205, 343, 552, 658
Hpy188III	TCNNGA	4 cut(s)	62, 256, 416, 745
Hpy8I	GTNNAC	1 cut(s)	652
HpyAV	CCTTC	3 cut(s)	109, 113, 706
HpyCH4III	ACNGT	2 cut(s)	436, 517
HpyCH4IV	ACGT	1 cut(s)	601
HpyCH4V	TGCA	9 cut(s)	248, 269, 431, 520, 533, 566, 736, 779, 785
HpyF10VI	GCNNNNNNNGC	4 cut(s)	65, 233, 572, 584
HpyF3I	CTNAG	3 cut(s)	60, 408, 624
HpySE526I	ACGT	1 cut(s)	601
Hsp92II	CATG	7 cut(s)	197, 300, 362, 431, 636, 711, 825
HspAI	GCGC	1 cut(s)	526
Ksp22I	TGATCA	2 cut(s)	484, 592
KspI	CCGCGG	1 cut(s)	113
Kzo9I	GATC	3 cut(s)	313, 484, 592
Lsp1109I	GCAGC	7 cut(s)	4, 184, 459, 532, 535, 545, 562
LweI	GCATC	3 cut(s)	4, 46, 766
MaeI	CTAG	5 cut(s)	185, 218, 311, 444, 773
MaeII	ACGT	1 cut(s)	601
MaeIII	GTNAC	2 cut(s)	436, 703
MalI	GATC	3 cut(s)	315, 486, 594
MboI	GATC	3 cut(s)	313, 484, 592
MboII	GAAGA	3 cut(s)	20, 298, 393
MfeI	CAATTG	1 cut(s)	729
MhlI	GDGCHC	1 cut(s)	795
MluCI	AATT	3 cut(s)	9, 729, 829
MlyI	GAGTC	2 cut(s)	613, 735
MnlI	CCTC	2 cut(s)	36, 793
MseI	TTAA	3 cut(s)	494, 543, 832
MslI	CAYNNNNRTG	2 cut(s)	515, 600
MspA1I	CMGCKG	2 cut(s)	112, 450
MunI	CAATTG	1 cut(s)	729
MvnI	CGCG	2 cut(s)	112, 528
MwoI	GCNNNNNNNGC	4 cut(s)	65, 233, 572, 584
NdeII	GATC	3 cut(s)	313, 484, 592
NlaIII	CATG	7 cut(s)	197, 300, 362, 431, 636, 711, 825
NmuCI	GTSAC	1 cut(s)	436
NspI	RCATGY	3 cut(s)	197, 300, 711
OliI	CACNNNNGTG	2 cut(s)	515, 600
PaeI	GCATGC	1 cut(s)	197
PceI	AGGCCT	1 cut(s)	684
PciI	ACATGT	2 cut(s)	296, 707
PfeI	GAWTC	2 cut(s)	105, 672
PkrI	GCNGC	7 cut(s)	19, 199, 449, 522, 525, 535, 577
PleI	GAGTC	2 cut(s)	613, 735
PpsI	GAGTC	2 cut(s)	613, 735
Ppu21I	YACGTR	1 cut(s)	602
PscI	ACATGT	2 cut(s)	296, 707
PshAI	GACNNNNGTC	1 cut(s)	724
Psp124BI	GAGCTC	1 cut(s)	795
PspFI	CCCAGC	1 cut(s)	232
RsaI	GTAC	1 cut(s)	653
RsaNI	GTAC	1 cut(s)	652
RseI	CAYNNNNRTG	2 cut(s)	515, 600
SacI	GAGCTC	1 cut(s)	795
SacII	CCGCGG	1 cut(s)	113
SaqAI	TTAA	3 cut(s)	494, 543, 832
SatI	GCNGC	7 cut(s)	18, 198, 448, 521, 524, 534, 576
Sau3AI	GATC	3 cut(s)	313, 484, 592
SchI	GAGTC	2 cut(s)	613, 735
SduI	GDGCHC	1 cut(s)	795
SfaNI	GCATC	3 cut(s)	4, 46, 766
Sfr303I	CCGCGG	1 cut(s)	113
SgrBI	CCGCGG	1 cut(s)	113
SmiMI	CAYNNNNRTG	2 cut(s)	515, 600
SmlI	CTYRAG	3 cut(s)	171, 743, 760
SmoI	CTYRAG	3 cut(s)	171, 743, 760
SphI	GCATGC	1 cut(s)	197
Sse9I	AATT	3 cut(s)	9, 729, 829
SseBI	AGGCCT	1 cut(s)	684
SsiI	CCGC	5 cut(s)	102, 110, 112, 450, 528
SspI	AATATT	1 cut(s)	85
SspMI	CTAG	5 cut(s)	185, 218, 311, 444, 773
SstI	GAGCTC	1 cut(s)	795
StuI	AGGCCT	1 cut(s)	684
TaaI	ACNGT	2 cut(s)	436, 517
TaiI	ACGT	1 cut(s)	604
TaqI	TCGA	2 cut(s)	146, 798
TasI	AATT	3 cut(s)	9, 729, 829
TatI	WGTACW	1 cut(s)	651
TfiI	GAWTC	2 cut(s)	105, 672
Tru1I	TTAA	3 cut(s)	494, 543, 832
Tru9I	TTAA	3 cut(s)	494, 543, 832
TscAI	CASTG	1 cut(s)	520
TseFI	GTSAC	1 cut(s)	436
TseI	GCWGC	7 cut(s)	17, 197, 447, 520, 523, 533, 575
Tsp45I	GTSAC	1 cut(s)	436
TspDTI	ATGAA	3 cut(s)	347, 496, 810
TspRI	CASTG	1 cut(s)	520
XceI	RCATGY	3 cut(s)	197, 300, 711
XspI	CTAG	5 cut(s)	185, 218, 311, 444, 773

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.