Rorug05G0078500

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009056
Physical Location & Seq
Forward (+)
6805932 .. 6806255
324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0078500.1

Sequence Viewer

Length: 324 bp
ATGAAGGTGATTGCTGCTTACATGCTCGCTCGATTGGGAGGGAACACCAATCCTTCTGCCAATGATCTGAAGAAACTTATCGAATCAGTTGGAGCTGAGGTTGATGGAGACAGAATCGAATTGTTCTTGTCCCAAGTCAATGGAAAAAATATCACCGAGCTAGTTGCATCCGGTAAAGAGAAGTTGGCATCTGTGCCTTCTGGCGGTGACGCTAGTGCTGCAGCTCCTCCTGCTATTGCTGAGGAGACAAAGGAAGAGAAGGTTGAAGAAGAGGAGGACGACTTTGATGAACCTCTTTTCGGTCTCTTTGGCCCCGACAACTAA

Protein Analysis

107

Amino Acids

11.29

Weight (kDa)

4.24

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 17 - 103 2.1e-19 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 203, 299
AgsI TTSAA 1 cut(s) 266
AluBI AGCT 3 cut(s) 95, 160, 224
AluI AGCT 3 cut(s) 95, 160, 224
Alw26I GTCTC 3 cut(s) 102, 239, 308
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 3 cut(s) 14, 218, 221
AspS9I GGNCC 1 cut(s) 311
AsuHPI GGTGA 3 cut(s) 19, 145, 218
BbvCI CCTCAGC 2 cut(s) 96, 240
BbvI GCAGC 2 cut(s) 205, 233
BccI CCATC 1 cut(s) 98
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BcoDI GTCTC 3 cut(s) 102, 239, 308
BfaI CTAG 2 cut(s) 161, 213
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 15, 219, 222
BlsI GCNGC 3 cut(s) 16, 220, 223
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 2 cut(s) 176, 197
Bpu10I CCTNAGC 2 cut(s) 96, 240
BsaI GGTCTC 1 cut(s) 308
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 299
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 203, 299
BseMII CTCAG 2 cut(s) 87, 231
BseRI GAGGAG 3 cut(s) 216, 257, 287
BseXI GCAGC 2 cut(s) 205, 233
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 203, 299
BsmAI GTCTC 3 cut(s) 102, 239, 308
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 308
Bsp143I GATC 1 cut(s) 64
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 2 cut(s) 88, 232
BspLI GGNNCC 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 308
BssMI GATC 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 249, 264
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 96, 240
BstF5I GGATG 1 cut(s) 167
BstKTI GATC 1 cut(s) 67
BstMAI GTCTC 3 cut(s) 102, 239, 308
BstMBI GATC 1 cut(s) 64
BstMWI GCNNNNNNNGC 2 cut(s) 218, 230
BstNSI RCATGY 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 205, 233
BstXI CCANNNNNNTGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 312
BtsCI GGATG 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 311
CseI GACGC 1 cut(s) 218
CviAII CATG 1 cut(s) 22
CviJI RGCY 4 cut(s) 95, 160, 224, 312
CviKI_1 RGCY 4 cut(s) 95, 160, 224, 312
DdeI CTNAG 2 cut(s) 96, 240
DpnI GATC 1 cut(s) 66
DpnII GATC 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 249, 264
EarI CTCTTC 2 cut(s) 249, 264
Eco31I GGTCTC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 89
FaeI CATG 1 cut(s) 25
FaiI YATR 1 cut(s) 23
FaqI GGGAC 1 cut(s) 115
FatI CATG 1 cut(s) 21
Fnu4HI GCNGC 3 cut(s) 15, 219, 222
FokI GGATG 1 cut(s) 154
Fsp4HI GCNGC 3 cut(s) 15, 219, 222
FspBI CTAG 2 cut(s) 161, 213
GluI GCNGC 3 cut(s) 15, 219, 222
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 218
Hin1II CATG 1 cut(s) 25
HinfI GANTC 2 cut(s) 83, 114
HpaII CCGG 1 cut(s) 171
HphI GGTGA 3 cut(s) 19, 145, 218
Hpy188I TCNGA 1 cut(s) 69
HpyAV CCTTC 3 cut(s) 63, 207, 253
HpyCH4V TGCA 2 cut(s) 167, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 218, 230
HpyF3I CTNAG 2 cut(s) 96, 240
Hsp92II CATG 1 cut(s) 25
Kzo9I GATC 1 cut(s) 64
LmnI GCTCC 2 cut(s) 92, 229
LpnPI CCDG 3 cut(s) 184, 186, 243
Lsp1109I GCAGC 2 cut(s) 205, 233
LweI GCATC 2 cut(s) 176, 197
MaeI CTAG 2 cut(s) 161, 213
MaeIII GTNAC 1 cut(s) 206
MalI GATC 1 cut(s) 66
MboI GATC 1 cut(s) 64
MboII GAAGA 4 cut(s) 82, 266, 278, 281
MluCI AATT 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 7 cut(s) 32, 91, 235, 237, 265, 268, 303
MspI CCGG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 218, 230
NdeII GATC 1 cut(s) 64
NlaIII CATG 1 cut(s) 25
NlaIV GGNNCC 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 206
NspI RCATGY 1 cut(s) 25
PfeI GAWTC 2 cut(s) 83, 114
PkrI GCNGC 3 cut(s) 16, 220, 223
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 1 cut(s) 311
PstI CTGCAG 1 cut(s) 223
SatI GCNGC 3 cut(s) 15, 219, 222
Sau3AI GATC 1 cut(s) 64
Sau96I GGNCC 1 cut(s) 311
SetI ASST 7 cut(s) 9, 97, 102, 162, 226, 264, 295
SfaNI GCATC 2 cut(s) 176, 197
SfcI CTRYAG 1 cut(s) 219
Sse9I AATT 1 cut(s) 119
SsiI CCGC 1 cut(s) 204
SspMI CTAG 2 cut(s) 161, 213
TaqI TCGA 3 cut(s) 31, 81, 117
TaqII GACCGA 1 cut(s) 290
TasI AATT 1 cut(s) 119
TfiI GAWTC 2 cut(s) 83, 114
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 3 cut(s) 14, 218, 221
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 17, 303
XceI RCATGY 1 cut(s) 25
XspI CTAG 2 cut(s) 161, 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.