Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009056

Physical Location & Seq

Genomic Coordinates Forward (+)

31539017 .. 31542210

Gene Structure 3194 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1216900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGGCGAAAAACCCAGTTCGAGTTCTTGTCACTGGTGCCGCTGGGCAAATAGGGTATGCTATTGTTCCGATGATTGCCAGGGGGGTCATGCTAGGCCCTGACCAGCCTGTAATTCTGCACATGCTTGATATTGAACCTGCAGCTGAGGCCCTGAATGGGGTGAAAATGGAACTGATTGATGCTGCCTTTCCTCTTCTCAAAGGTGTTGTTGCTACAACAGATGCTCAGGAAGCTTGTAAAGATGTCAATATTGCTATTATGGTCGGCGGGTTCCCGCGGAAGGAAGGTATGGAAAGAAAGGATGTGATGTCGAAAAATGTATCAATCTACAAGGCTCAAGCTTCAGCACTAGAAAAGCATGCTGCTTCAGATGTCAAGGTTCTGGTGGTTGCCAACCCAGCAAACACTAATGCACTCATCTTGAAAGAATATGCACCTAAAATCCCAGAGAAGAACATCACATGTCTTACAAGACTAGATCATAACAGAGCTTTGGGACAAATCTCCGAGAAGCTAAACGTTCACGTTGGTGACGTGAAGAATGTTATCATCTGGGGCAATCACTCTTCAACTCAGTATCCTGATGTTAATCATGCAAATGTCACAACTAGCAGTGAAGAGAAACCTGTCAGGGAACTTGTGGCTAATGATCAATGGTTAAATACCGAGTTCATCACCACTGTGCAGCAGCGCGGTGCAGCCATTATTAAGGCTCGGAAGTTATCAAGTGCATTATCTGCTGCAAGTTCCGCTTGTGATCACATACGTGATTGGGTTCTTGGGACTCCTAAGGGGACATGGGTTTCAATGGGAGTGTACTCTGATGGTTCTTATGGAATTCAGAAAGGCCTAATTTACTCCTTCCCTGTTACATGTGAGAAAGGACAATGGTCAATTGTGCAGGGACTCAAGATTGACGAGTTCTCGAGGGGGAAGTTGGATGCAACTGCAAAAGAGCTCATTGAGGAGAAATCGTTGGCCTACTCATGCCTTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009987 GO:0015980 GO:0016020 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0034641 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04964 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200 map04964

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

333

Amino Acids

35.95

Weight (kDa)

7.64

Isoelectric Point (pI)

31.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	6 - 153	1.9e-31	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	157 - 325	7.7e-47	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56720
fragaria_vesca	FvH4_3g14020 FvH4_3g14020 FvH4_3g14020
malus_domestica	MD05G1238800.v1.1 MD10G1216900.v1.1
prunus_persica	Prupe.4G127500_v2.0.a1 Prupe.4G127500_v2.0.a1
pyrus_communis	pycom05g21480 pycom10g18290
rosa_chinensis	RchiOBHm_Chr5g0023331
rosa_laevigata	RLG00000032714
rosa_multiflora	Rmu_sc0004693.1_g000004 Rmu_sc0009954.1_g000015
rosa_roxburghii	Rroxscaffold_1G00056000
rosa_rugosa	Rorug05G0078400 Rorug05G0078500
rosa_samantha	Rh5AG165000 Rh5BG164500 Rh5CG179400 Rh5DG166000
rosa_wichuraiana	Rw5G014870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	145
AccB1I	GGYRCC	1 cut(s)	35
AccII	CGCG	2 cut(s)	277, 693
AciI	CCGC	6 cut(s)	39, 267, 275, 277, 693, 750
AclI	AACGTT	1 cut(s)	519
AcsI	RAATTY	1 cut(s)	837
AcuI	CTGAAG	2 cut(s)	327, 351
AfaI	GTAC	1 cut(s)	818
AfiI	CCNNNNNNNGG	3 cut(s)	156, 157, 280
AflIII	ACRYGT	2 cut(s)	461, 872
AgsI	TTSAA	4 cut(s)	134, 424, 570, 807
AjiI	CACGTC	1 cut(s)	535
AjnI	CCWGG	1 cut(s)	77
AleI	CACNNNNGTG	3 cut(s)	528, 680, 765
AluBI	AGCT	6 cut(s)	143, 233, 341, 491, 514, 958
AluI	AGCT	6 cut(s)	143, 233, 341, 491, 514, 958
Alw21I	GWGCWC	1 cut(s)	960
Ama87I	CYCGRG	1 cut(s)	925
AoxI	GGCC	4 cut(s)	94, 147, 847, 978
ApeKI	GCWGC	7 cut(s)	140, 182, 362, 685, 688, 698, 740
ApoI	RAATTY	1 cut(s)	837
AspLEI	GCGC	1 cut(s)	693
AspS9I	GGNCC	2 cut(s)	95, 148
AsuHPI	GGTGA	3 cut(s)	172, 542, 667
AvaI	CYCGRG	1 cut(s)	925
AxyI	CCTNAGG	1 cut(s)	789
BanI	GGYRCC	1 cut(s)	35
BanII	GRGCYC	1 cut(s)	960
Bbv12I	GWGCWC	1 cut(s)	960
BbvCI	CCTCAGC	1 cut(s)	144
BbvI	GCAGC	7 cut(s)	152, 169, 349, 697, 700, 710, 727
BccI	CCATC	1 cut(s)	818
BciT130I	CCWGG	1 cut(s)	79
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	2 cut(s)	649, 757
BfaI	CTAG	4 cut(s)	92, 350, 476, 609
BfmI	CTRYAG	1 cut(s)	138
BfuAI	ACCTGC	1 cut(s)	145
BfuI	GTATCC	1 cut(s)	588
BisI	GCNGC	8 cut(s)	39, 141, 183, 363, 686, 689, 699, 741
BlsI	GCNGC	8 cut(s)	40, 142, 184, 364, 687, 690, 700, 742
Bme1390I	CCNGG	1 cut(s)	79
BmeT110I	CYCGRG	1 cut(s)	925
BmgBI	CACGTC	1 cut(s)	535
BmgT120I	GGNCC	2 cut(s)	95, 148
BmiI	GGNNCC	2 cut(s)	37, 272
BmrFI	CCNGG	1 cut(s)	79
BmrI	ACTGGG	1 cut(s)	8
BmsI	GCATC	3 cut(s)	169, 211, 931
BmuI	ACTGGG	1 cut(s)	8
BoxI	GACNNNNGTC	1 cut(s)	889
Bpu10I	CCTNAGC	2 cut(s)	144, 225
BpuEI	CTTGAG	2 cut(s)	321, 893
BsaAI	YACGTR	1 cut(s)	767
BsaBI	GATNNNNATC	1 cut(s)	588
BsaJI	CCNNGG	2 cut(s)	78, 275
BsaXI	ACNNNNNCTCC	2 cut(s)	959, 989
Bsc4I	CCNNNNNNNGG	3 cut(s)	156, 157, 280
Bse1I	ACTGG	2 cut(s)	14, 37
Bse21I	CCTNAGG	1 cut(s)	789
Bse8I	GATNNNNATC	1 cut(s)	588
BseBI	CCWGG	1 cut(s)	79
BseDI	CCNNGG	2 cut(s)	78, 275
BseGI	GGATG	2 cut(s)	307, 946
BseJI	GATNNNNATC	1 cut(s)	588
BseLI	CCNNNNNNNGG	3 cut(s)	156, 157, 280
BseMII	CTCAG	3 cut(s)	135, 239, 587
BseNI	ACTGG	2 cut(s)	14, 37
BseRI	GAGGAG	1 cut(s)	980
BseXI	GCAGC	7 cut(s)	152, 169, 349, 697, 700, 710, 727
BseYI	CCCAGC	2 cut(s)	41, 397
BsgI	GTGCAG	4 cut(s)	101, 704, 717, 920
Bsh1236I	CGCG	2 cut(s)	277, 693
BshFI	GGCC	4 cut(s)	96, 149, 849, 980
BshNI	GGYRCC	1 cut(s)	35
BsiHKAI	GWGCWC	1 cut(s)	960
BsiHKCI	CYCGRG	1 cut(s)	925
BslFI	GGGAC	4 cut(s)	510, 796, 808, 918
BslI	CCNNNNNNNGG	3 cut(s)	156, 157, 280
BsmFI	GGGAC	4 cut(s)	510, 796, 808, 918
BsnI	GGCC	4 cut(s)	96, 149, 849, 980
BsoBI	CYCGRG	1 cut(s)	925
Bsp1286I	GDGCHC	1 cut(s)	960
Bsp143I	GATC	3 cut(s)	478, 649, 757
BspACI	CCGC	6 cut(s)	39, 267, 275, 277, 693, 750
BspANI	GGCC	4 cut(s)	96, 149, 849, 980
BspCNI	CTCAG	3 cut(s)	136, 238, 586
BspFNI	CGCG	2 cut(s)	277, 693
BspLI	GGNNCC	2 cut(s)	37, 272
BspMAI	CTGCAG	1 cut(s)	142
BspMI	ACCTGC	1 cut(s)	145
BspT107I	GGYRCC	1 cut(s)	35
BsrI	ACTGG	2 cut(s)	14, 37
BssECI	CCNNGG	2 cut(s)	78, 275
BssMI	GATC	3 cut(s)	478, 649, 757
Bst2UI	CCWGG	1 cut(s)	79
Bst4CI	ACNGT	1 cut(s)	682
Bst6I	CTCTTC	3 cut(s)	198, 571, 612
BstAPI	GCANNNNNTGC	1 cut(s)	737
BstBAI	YACGTR	1 cut(s)	767
BstC8I	GCNNGC	1 cut(s)	360
BstDEI	CTNAG	4 cut(s)	144, 225, 573, 789
BstDSI	CCRYGG	1 cut(s)	275
BstF5I	GGATG	2 cut(s)	307, 946
BstFNI	CGCG	2 cut(s)	277, 693
BstHHI	GCGC	1 cut(s)	693
BstKTI	GATC	3 cut(s)	481, 652, 760
BstMBI	GATC	3 cut(s)	478, 649, 757
BstMWI	GCNNNNNNNGC	5 cut(s)	146, 230, 398, 737, 749
BstNI	CCWGG	1 cut(s)	79
BstNSI	RCATGY	4 cut(s)	124, 362, 465, 876
BstPAI	GACNNNNGTC	1 cut(s)	889
BstSCI	CCNGG	1 cut(s)	77
BstSFI	CTRYAG	1 cut(s)	138
BstUI	CGCG	2 cut(s)	277, 693
BstV1I	GCAGC	7 cut(s)	152, 169, 349, 697, 700, 710, 727
Bsu36I	CCTNAGG	1 cut(s)	789
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	4 cut(s)	96, 149, 849, 980
BtgI	CCRYGG	1 cut(s)	275
BtrI	CACGTC	1 cut(s)	535
BtsCI	GGATG	2 cut(s)	307, 946
BtsI	GCAGTG	1 cut(s)	619
BtsIMutI	CAGTG	3 cut(s)	30, 619, 678
BveI	ACCTGC	1 cut(s)	145
Cac8I	GCNNGC	1 cut(s)	360
CfoI	GCGC	1 cut(s)	693
Cfr13I	GGNCC	2 cut(s)	95, 148
Cfr42I	CCGCGG	1 cut(s)	278
Csp6I	GTAC	1 cut(s)	817
CviAII	CATG	8 cut(s)	88, 121, 359, 462, 593, 798, 873, 987
CviQI	GTAC	1 cut(s)	817
DdeI	CTNAG	4 cut(s)	144, 225, 573, 789
DpnI	GATC	3 cut(s)	480, 651, 759
DpnII	GATC	3 cut(s)	478, 649, 757
Eam1104I	CTCTTC	3 cut(s)	198, 571, 612
EarI	CTCTTC	3 cut(s)	198, 571, 612
Ecl136II	GAGCTC	1 cut(s)	958
Eco147I	AGGCCT	1 cut(s)	849
Eco24I	GRGCYC	1 cut(s)	960
Eco53kI	GAGCTC	1 cut(s)	958
Eco57I	CTGAAG	2 cut(s)	327, 351
Eco81I	CCTNAGG	1 cut(s)	789
Eco88I	CYCGRG	1 cut(s)	925
EcoICRI	GAGCTC	1 cut(s)	958
EcoO109I	RGGNCCY	2 cut(s)	95, 148
EcoRI	GAATTC	1 cut(s)	837
EcoRII	CCWGG	1 cut(s)	77
EcoT38I	GRGCYC	1 cut(s)	960
FaeI	CATG	8 cut(s)	91, 124, 362, 465, 596, 801, 876, 990
FalI	AAGNNNNNCTT	2 cut(s)	736, 768
FaqI	GGGAC	4 cut(s)	510, 796, 808, 918
FatI	CATG	8 cut(s)	87, 120, 358, 461, 592, 797, 872, 986
FauI	CCCGC	2 cut(s)	260, 282
FbaI	TGATCA	2 cut(s)	649, 757
Fnu4HI	GCNGC	8 cut(s)	39, 141, 183, 363, 686, 689, 699, 741
FokI	GGATG	2 cut(s)	314, 953
FriOI	GRGCYC	1 cut(s)	960
Fsp4HI	GCNGC	8 cut(s)	39, 141, 183, 363, 686, 689, 699, 741
FspBI	CTAG	4 cut(s)	92, 350, 476, 609
GlaI	GCGC	1 cut(s)	692
GluI	GCNGC	8 cut(s)	39, 141, 183, 363, 686, 689, 699, 741
GsaI	CCCAGC	2 cut(s)	45, 401
HaeIII	GGCC	4 cut(s)	96, 149, 849, 980
HhaI	GCGC	1 cut(s)	693
Hin1II	CATG	8 cut(s)	91, 124, 362, 465, 596, 801, 876, 990
Hin6I	GCGC	1 cut(s)	691
HinP1I	GCGC	1 cut(s)	691
HindIII	AAGCTT	2 cut(s)	231, 339
HinfI	GANTC	2 cut(s)	784, 906
HphI	GGTGA	3 cut(s)	172, 542, 667
Hpy166II	GTNNAC	2 cut(s)	523, 817
Hpy188I	TCNGA	6 cut(s)	69, 370, 508, 717, 823, 843
Hpy188III	TCNNGA	5 cut(s)	227, 421, 581, 910, 925
Hpy8I	GTNNAC	2 cut(s)	523, 817
HpyAV	CCTTC	3 cut(s)	274, 278, 871
HpyCH4III	ACNGT	1 cut(s)	682
HpyCH4IV	ACGT	4 cut(s)	519, 525, 534, 766
HpyF10VI	GCNNNNNNNGC	5 cut(s)	146, 230, 398, 737, 749
HpyF3I	CTNAG	4 cut(s)	144, 225, 573, 789
HpySE526I	ACGT	4 cut(s)	519, 525, 534, 766
Hsp92II	CATG	8 cut(s)	91, 124, 362, 465, 596, 801, 876, 990
HspAI	GCGC	1 cut(s)	691
Ksp22I	TGATCA	2 cut(s)	649, 757
KspI	CCGCGG	1 cut(s)	278
Kzo9I	GATC	3 cut(s)	478, 649, 757
Lsp1109I	GCAGC	7 cut(s)	152, 169, 349, 697, 700, 710, 727
LweI	GCATC	3 cut(s)	169, 211, 931
MaeI	CTAG	4 cut(s)	92, 350, 476, 609
MaeII	ACGT	4 cut(s)	519, 525, 534, 766
MaeIII	GTNAC	4 cut(s)	28, 530, 601, 868
MalI	GATC	3 cut(s)	480, 651, 759
MboI	GATC	3 cut(s)	478, 649, 757
MboII	GAAGA	5 cut(s)	185, 463, 550, 558, 629
MfeI	CAATTG	1 cut(s)	894
MhlI	GDGCHC	1 cut(s)	960
MluCI	AATT	5 cut(s)	111, 837, 852, 894, 994
MlyI	GAGTC	2 cut(s)	778, 900
MmeI	TCCRAC	1 cut(s)	918
MnlI	CCTC	4 cut(s)	139, 201, 921, 958
MseI	TTAA	4 cut(s)	588, 659, 708, 993
MslI	CAYNNNNRTG	4 cut(s)	528, 597, 680, 765
MspA1I	CMGCKG	3 cut(s)	41, 143, 277
MspR9I	CCNGG	1 cut(s)	79
MunI	CAATTG	1 cut(s)	894
MvaI	CCWGG	1 cut(s)	79
MvnI	CGCG	2 cut(s)	277, 693
MwoI	GCNNNNNNNGC	5 cut(s)	146, 230, 398, 737, 749
NdeII	GATC	3 cut(s)	478, 649, 757
NlaIII	CATG	8 cut(s)	91, 124, 362, 465, 596, 801, 876, 990
NlaIV	GGNNCC	2 cut(s)	37, 272
NmuCI	GTSAC	3 cut(s)	28, 530, 601
NspI	RCATGY	4 cut(s)	124, 362, 465, 876
OliI	CACNNNNGTG	3 cut(s)	528, 680, 765
PaeI	GCATGC	1 cut(s)	362
PaeR7I	CTCGAG	1 cut(s)	925
PceI	AGGCCT	1 cut(s)	849
PciI	ACATGT	2 cut(s)	461, 872
PcsI	WCGNNNNNNNCGW	1 cut(s)	531
PkrI	GCNGC	8 cut(s)	40, 142, 184, 364, 687, 690, 700, 742
PleI	GAGTC	2 cut(s)	778, 900
PpsI	GAGTC	2 cut(s)	778, 900
Ppu21I	YACGTR	1 cut(s)	767
PscI	ACATGT	2 cut(s)	461, 872
PshAI	GACNNNNGTC	1 cut(s)	889
Psp124BI	GAGCTC	1 cut(s)	960
Psp1406I	AACGTT	1 cut(s)	519
Psp6I	CCWGG	1 cut(s)	77
PspFI	CCCAGC	2 cut(s)	41, 397
PspGI	CCWGG	1 cut(s)	77
PspN4I	GGNNCC	2 cut(s)	37, 272
PspPI	GGNCC	2 cut(s)	95, 148
PstI	CTGCAG	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	143
RsaI	GTAC	1 cut(s)	818
RsaNI	GTAC	1 cut(s)	817
RseI	CAYNNNNRTG	4 cut(s)	528, 597, 680, 765
SacI	GAGCTC	1 cut(s)	960
SacII	CCGCGG	1 cut(s)	278
SaqAI	TTAA	4 cut(s)	588, 659, 708, 993
SatI	GCNGC	8 cut(s)	39, 141, 183, 363, 686, 689, 699, 741
Sau3AI	GATC	3 cut(s)	478, 649, 757
Sau96I	GGNCC	2 cut(s)	95, 148
SchI	GAGTC	2 cut(s)	778, 900
ScrFI	CCNGG	1 cut(s)	79
SduI	GDGCHC	1 cut(s)	960
SfaNI	GCATC	3 cut(s)	169, 211, 931
SfcI	CTRYAG	1 cut(s)	138
Sfr274I	CTCGAG	1 cut(s)	925
Sfr303I	CCGCGG	1 cut(s)	278
SgrBI	CCGCGG	1 cut(s)	278
SlaI	CTCGAG	1 cut(s)	925
SmiMI	CAYNNNNRTG	4 cut(s)	528, 597, 680, 765
SmlI	CTYRAG	3 cut(s)	336, 908, 925
SmoI	CTYRAG	3 cut(s)	336, 908, 925
SphI	GCATGC	1 cut(s)	362
Sse9I	AATT	5 cut(s)	111, 837, 852, 894, 994
SseBI	AGGCCT	1 cut(s)	849
SsiI	CCGC	6 cut(s)	39, 267, 275, 277, 693, 750
SspI	AATATT	1 cut(s)	250
SspMI	CTAG	4 cut(s)	92, 350, 476, 609
SstI	GAGCTC	1 cut(s)	960
StuI	AGGCCT	1 cut(s)	849
StyD4I	CCNGG	1 cut(s)	77
TaaI	ACNGT	1 cut(s)	682
TaiI	ACGT	4 cut(s)	522, 528, 537, 769
TaqI	TCGA	3 cut(s)	19, 311, 926
TasI	AATT	5 cut(s)	111, 837, 852, 894, 994
TatI	WGTACW	1 cut(s)	816
TauI	GCSGC	1 cut(s)	41
Tru1I	TTAA	4 cut(s)	588, 659, 708, 993
Tru9I	TTAA	4 cut(s)	588, 659, 708, 993
TscAI	CASTG	3 cut(s)	37, 619, 685
TseFI	GTSAC	3 cut(s)	28, 530, 601
TseI	GCWGC	7 cut(s)	140, 182, 362, 685, 688, 698, 740
Tsp45I	GTSAC	3 cut(s)	28, 530, 601
TspDTI	ATGAA	1 cut(s)	661
TspRI	CASTG	3 cut(s)	37, 619, 685
XapI	RAATTY	1 cut(s)	837
XceI	RCATGY	4 cut(s)	124, 362, 465, 876
XhoI	CTCGAG	1 cut(s)	925
XspI	CTAG	4 cut(s)	92, 350, 476, 609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.