MD05G1252700.v1.1

Cytochrome c6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013171
Physical Location & Seq
Reverse (-)
38414923 .. 38417213
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1252700.v1.1.491

Sequence Viewer

Length: 522 bp
ATGCTGCTTACATCTCTGGTATCTAATAGCCCTAATGGTACCTTTTGCCTCCCAAAGGGTGCAGTGAAGCAAGGAGGAGGACACAACCCAATTCCTCAAAAGCAAGAAGAATGGAAGTTTCTCAAGAGCTTGGCTCCTCCCTTAATGGCTGCAGTTCTGACTTTATCTCCACTCTCAATCACCCCTGTCTCGCATGCGCAAGCCATTGATGTTCAAAGAGGAGCTGCATTGTTTCGTAGAACTTGCATTGGATGTCATGATGCAGGTGGAAACATAATACAACCAGGTGCAACACTCTTCACTAAAGACCTACAGAGAAACGGAGTTGATACAGAAGAGGAGATATACCGTGTTACTTACTTTGGAAAGGGAAGAATGCCGGGTTTTGGTGAGAAATGCGCGCCAAGGGGCCAGTGCACTTTTGGAGCTCGTTTGCAGGATGAAGAGATAAAGATTTTGGCTGAGTTTGTGAAATTACAGGCTGATCAAAACTGGCCCAACATTTTAATTGAAGACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 map00195
Pfam Domains
Protein Families
Cytochrome_CBB3

Protein Analysis

174

Amino Acids

18.89

Weight (kDa)

8.29

Isoelectric Point (pI)

55.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_CBB3 PF13442 71 - 133 2e-09 Cytochrome C oxidase, cbb3-type, subunit III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45040
fragaria_vesca FvH4_3g12220 FvH4_3g12220
malus_domestica MD05G1252700.v1.1 MD10G1233800.v1.1
prunus_persica Prupe.4G111200_v2.0.a1
pyrus_communis pycom05g23080
rosa_chinensis RchiOBHm_Chr5g0020011
rosa_laevigata RLG00000032493
rosa_multiflora Rmu_sc0002250.1_g000004
rosa_roxburghii Rroxscaffold_1G00058180
rosa_rugosa Rorug05G0056300 Rorug05G0056300
rosa_samantha Rh5AG146400 Rh5BG145900 Rh5CG157300 Rh5DG145300
rosa_wichuraiana Rw5G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 198
Acc36I ACCTGC 1 cut(s) 254
Acc65I GGTACC 1 cut(s) 38
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 1 cut(s) 401
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 55, 386
AgsI TTSAA 2 cut(s) 215, 512
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 3 cut(s) 129, 224, 428
AluI AGCT 3 cut(s) 129, 224, 428
Alw21I GWGCWC 2 cut(s) 419, 430
Alw26I GTCTC 1 cut(s) 193
Alw44I GTGCAC 1 cut(s) 415
AoxI GGCC 2 cut(s) 409, 494
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 3 cut(s) 4, 149, 224
Asp718I GGTACC 1 cut(s) 38
AspLEI GCGC 3 cut(s) 199, 401, 403
AspS9I GGNCC 2 cut(s) 409, 495
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 2 cut(s) 172, 401
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 430
Bbv12I GWGCWC 2 cut(s) 419, 430
BbvI GCAGC 2 cut(s) 136, 211
BciT130I CCWGG 1 cut(s) 285
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 193
BfmI CTRYAG 2 cut(s) 150, 311
BfuAI ACCTGC 1 cut(s) 254
BisI GCNGC 3 cut(s) 5, 150, 225
BlsI GCNGC 3 cut(s) 6, 151, 226
Bme1390I CCNGG 2 cut(s) 285, 381
BmgT120I GGNCC 2 cut(s) 409, 495
BmiI GGNNCC 3 cut(s) 40, 135, 410
BmrFI CCNGG 2 cut(s) 285, 381
BmsI GCATC 1 cut(s) 250
BplI GAGNNNNNCTC 2 cut(s) 118, 150
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 386
Bse1I ACTGG 2 cut(s) 412, 497
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 2 cut(s) 257, 445
BseLI CCNNNNNNNGG 2 cut(s) 55, 386
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 2 cut(s) 412, 497
BsePI GCGCGC 1 cut(s) 399
BseRI GAGGAG 4 cut(s) 90, 126, 234, 353
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 2 cut(s) 136, 211
BsgI GTGCAG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 411, 496
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 419, 430
BsiSI CCGG 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 55, 386
BsmAI GTCTC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 2 cut(s) 411, 496
Bsp1286I GDGCHC 2 cut(s) 419, 430
Bsp143I GATC 1 cut(s) 484
BspANI GGCC 2 cut(s) 411, 496
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 401
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 3 cut(s) 40, 135, 410
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 38
BsrI ACTGG 2 cut(s) 412, 497
BssECI CCNNGG 1 cut(s) 404
BssHII GCGCGC 1 cut(s) 399
BssMI GATC 1 cut(s) 484
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 3 cut(s) 302, 330, 438
BstC8I GCNNGC 3 cut(s) 195, 201, 401
BstDEI CTNAG 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 257, 445
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 3 cut(s) 199, 401, 403
BstKTI GATC 1 cut(s) 487
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 1 cut(s) 484
BstNI CCWGG 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 2 cut(s) 283, 379
BstSFI CTRYAG 2 cut(s) 150, 311
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 136, 211
BsuRI GGCC 2 cut(s) 411, 496
BtsCI GGATG 2 cut(s) 257, 445
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 419
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 3 cut(s) 195, 201, 401
CciI TCATGA 1 cut(s) 256
CfoI GCGC 3 cut(s) 199, 401, 403
Cfr13I GGNCC 2 cut(s) 409, 495
CsiI ACCWGGT 1 cut(s) 283
Csp6I GTAC 1 cut(s) 39
CviAII CATG 2 cut(s) 194, 257
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 1 cut(s) 462
DpnI GATC 1 cut(s) 486
DpnII GATC 1 cut(s) 484
Eam1104I CTCTTC 3 cut(s) 302, 330, 438
EarI CTCTTC 3 cut(s) 302, 330, 438
Ecl136II GAGCTC 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 430
Eco53kI GAGCTC 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 197, 260
FaiI YATR 4 cut(s) 195, 258, 275, 346
FatI CATG 2 cut(s) 193, 256
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 3 cut(s) 5, 150, 225
FokI GGATG 2 cut(s) 264, 452
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 3 cut(s) 5, 150, 225
FspI TGCGCA 1 cut(s) 198
GlaI GCGC 3 cut(s) 198, 400, 402
GluI GCNGC 3 cut(s) 5, 150, 225
HaeIII GGCC 2 cut(s) 411, 496
HapII CCGG 1 cut(s) 380
HhaI GCGC 3 cut(s) 199, 401, 403
Hin1II CATG 2 cut(s) 197, 260
Hin6I GCGC 3 cut(s) 197, 399, 401
HinP1I GCGC 3 cut(s) 197, 399, 401
HpaII CCGG 1 cut(s) 380
HphI GGTGA 2 cut(s) 172, 401
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 2 cut(s) 124, 257
Hpy8I GTNNAC 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 8 cut(s) 62, 152, 227, 246, 263, 290, 417, 436
HpyF3I CTNAG 1 cut(s) 462
Hsp92II CATG 2 cut(s) 197, 260
HspAI GCGC 3 cut(s) 197, 399, 401
KpnI GGTACC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 1 cut(s) 484
LmnI GCTCC 3 cut(s) 139, 221, 425
Lsp1109I GCAGC 2 cut(s) 136, 211
LweI GCATC 1 cut(s) 250
MabI ACCWGGT 1 cut(s) 283
MaeIII GTNAC 1 cut(s) 352
MalI GATC 1 cut(s) 486
MboI GATC 1 cut(s) 484
MboII GAAGA 5 cut(s) 119, 289, 347, 384, 455
MhlI GDGCHC 2 cut(s) 419, 430
MluCI AATT 3 cut(s) 90, 473, 507
MnlI CCTC 7 cut(s) 59, 68, 71, 105, 147, 212, 331
MseI TTAA 2 cut(s) 143, 506
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 2 cut(s) 285, 381
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 401
NciI CCSGG 1 cut(s) 381
NdeII GATC 1 cut(s) 484
NlaIII CATG 2 cut(s) 197, 260
NlaIV GGNNCC 3 cut(s) 40, 135, 410
NsbI TGCGCA 1 cut(s) 198
NspI RCATGY 1 cut(s) 197
PaeI GCATGC 1 cut(s) 197
PagI TCATGA 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 254
PauI GCGCGC 1 cut(s) 399
PctI GAATGC 1 cut(s) 381
PkrI GCNGC 3 cut(s) 6, 151, 226
Psp124BI GAGCTC 1 cut(s) 430
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 40, 135, 410
PspPI GGNCC 2 cut(s) 409, 495
PstI CTGCAG 1 cut(s) 154
PteI GCGCGC 1 cut(s) 399
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SacI GAGCTC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 143, 506
SatI GCNGC 3 cut(s) 5, 150, 225
Sau3AI GATC 1 cut(s) 484
Sau96I GGNCC 2 cut(s) 409, 495
ScrFI CCNGG 2 cut(s) 285, 381
SduI GDGCHC 2 cut(s) 419, 430
SetI ASST 7 cut(s) 44, 131, 226, 268, 289, 312, 430
SexAI ACCWGGT 1 cut(s) 283
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 2 cut(s) 150, 311
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
SphI GCATGC 1 cut(s) 197
Sse9I AATT 3 cut(s) 90, 473, 507
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 2 cut(s) 283, 379
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 350
TasI AATT 3 cut(s) 90, 473, 507
Tru1I TTAA 2 cut(s) 143, 506
Tru9I TTAA 2 cut(s) 143, 506
TscAI CASTG 2 cut(s) 69, 419
TseI GCWGC 3 cut(s) 4, 149, 224
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 2 cut(s) 69, 419
VneI GTGCAC 1 cut(s) 415
XagI CCTNNNNNAGG 1 cut(s) 53
XceI RCATGY 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.