Rroxscaffold_1G00058180

Cytochrome c6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013171
Physical Location & Seq
Reverse (-)
80054470 .. 80057301
2832 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00058180.1

Sequence Viewer

Length: 591 bp
ATGTATCTTGGTATTTTCCATTCTGTTCCTGCAATTACACCAACATTATCACTTTGTCTTCCATTAAAGAAAGGGATGATGCAGCTTACGTCTTTGATATCAAACACCAAAAATGGTGCCTATTTCTTCCCAAAGTGTGCAGTGAAGCAACAACACAACCCAATTGCTCAGAAACAAGAACAATGTAAGCTTTTAAGGAGCTTGGCTCCACCTCTAATGGCTGCAGCTCTGGCCTTATCTCCAATCTGCGTTACTCCTGTCTCACATGCCCAAACCATCGATGTTCAGAGAGGAGCTGCGTTGTTTCGTACTACTTGCATTGGATGTCATGATGCAGGTGGAAACATAATACAACCAGGTGCAACACTCTTTACTAAAGATCTACAAAGAAATGGAGTTGACACAGAGGAGGAGATATATCATGTTACTTACTTTGGCAAGGGAAGAATGCCAGGTTTTGGTCAGAACTGCACACCAAAGGGCCAATGCACATTTGGAGCTCGATTGCAGGACGAGGACATTAAGCTTTTAGCTGAGTTTGTCAAGTTACAGGCTGATCAAGACTGGAGAAATAATCCAAGTAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 map00195
Pfam Domains
Protein Families
Cytochrome_CBB3

Protein Analysis

196

Amino Acids

21.49

Weight (kDa)

8.63

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_CBB3 PF13442 95 - 160 6.6e-10 Cytochrome C oxidase, cbb3-type, subunit III
Cytochrom_C PF00034 95 - 182 6.7e-06 Cytochrome c
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45040
fragaria_vesca FvH4_3g12220 FvH4_3g12220
malus_domestica MD05G1252700.v1.1 MD10G1233800.v1.1
prunus_persica Prupe.4G111200_v2.0.a1
pyrus_communis pycom05g23080
rosa_chinensis RchiOBHm_Chr5g0020011
rosa_laevigata RLG00000032493
rosa_multiflora Rmu_sc0002250.1_g000004
rosa_roxburghii Rroxscaffold_1G00058180
rosa_rugosa Rorug05G0056300 Rorug05G0056300
rosa_samantha Rh5AG146400 Rh5BG145900 Rh5CG157300 Rh5DG145300
rosa_wichuraiana Rw5G012930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 458
AfaI GTAC 1 cut(s) 310
AfiI CCNNNNNNNGG 1 cut(s) 458
AjnI CCWGG 2 cut(s) 355, 451
AluBI AGCT 8 cut(s) 85, 190, 201, 227, 296, 500, 526, 533
AluI AGCT 8 cut(s) 85, 190, 201, 227, 296, 500, 526, 533
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 1 cut(s) 265
AoxI GGCC 2 cut(s) 231, 481
ApeKI GCWGC 4 cut(s) 82, 221, 224, 296
AspS9I GGNCC 1 cut(s) 481
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 502
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 4 cut(s) 94, 208, 236, 283
BccI CCATC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 357, 453
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 1 cut(s) 265
BfmI CTRYAG 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 326
BglII AGATCT 1 cut(s) 379
BisI GCNGC 4 cut(s) 83, 222, 225, 297
BlsI GCNGC 4 cut(s) 84, 223, 226, 298
Bme1390I CCNGG 2 cut(s) 357, 453
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 2 cut(s) 118, 207
BmrFI CCNGG 2 cut(s) 357, 453
BmsI GCATC 2 cut(s) 69, 322
BpiI GAAGAC 1 cut(s) 50
BplI GAGNNNNNCTC 2 cut(s) 190, 222
BpmI CTGGAG 1 cut(s) 586
Bsa29I ATCGAT 1 cut(s) 279
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse1I ACTGG 1 cut(s) 569
BseBI CCWGG 2 cut(s) 357, 453
BseCI ATCGAT 1 cut(s) 279
BseGI GGATG 2 cut(s) 81, 329
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMII CTCAG 2 cut(s) 182, 525
BseNI ACTGG 1 cut(s) 569
BseRI GAGGAG 3 cut(s) 306, 422, 425
BseXI GCAGC 4 cut(s) 94, 208, 236, 283
BsgI GTGCAG 2 cut(s) 159, 454
BshFI GGCC 2 cut(s) 233, 483
BshNI GGYRCC 1 cut(s) 116
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 502
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 2 cut(s) 233, 483
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 2 cut(s) 379, 556
BspANI GGCC 2 cut(s) 233, 483
BspCNI CTCAG 2 cut(s) 181, 526
BspDI ATCGAT 1 cut(s) 279
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 118, 207
BspMAI CTGCAG 1 cut(s) 226
BspMI ACCTGC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 569
BssMI GATC 2 cut(s) 379, 556
Bst2UI CCWGG 2 cut(s) 357, 453
BstDEI CTNAG 2 cut(s) 168, 534
BstF5I GGATG 2 cut(s) 81, 329
BstKTI GATC 2 cut(s) 382, 559
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 2 cut(s) 379, 556
BstMWI GCNNNNNNNGC 1 cut(s) 230
BstNI CCWGG 2 cut(s) 357, 453
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 355, 451
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 4 cut(s) 94, 208, 236, 283
BstV2I GAAGAC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 2 cut(s) 233, 483
BsuTUI ATCGAT 1 cut(s) 279
BtsCI GGATG 2 cut(s) 81, 329
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
BveI ACCTGC 1 cut(s) 326
CciI TCATGA 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 481
ClaI ATCGAT 1 cut(s) 279
CsiI ACCWGGT 1 cut(s) 355
Csp6I GTAC 1 cut(s) 309
CviAII CATG 3 cut(s) 266, 329, 422
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 2 cut(s) 168, 534
DpnI GATC 2 cut(s) 381, 558
DpnII GATC 2 cut(s) 379, 556
Ecl136II GAGCTC 1 cut(s) 500
Eco24I GRGCYC 1 cut(s) 502
Eco32I GATATC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 500
EcoRII CCWGG 2 cut(s) 355, 451
EcoRV GATATC 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 502
FaeI CATG 3 cut(s) 269, 332, 425
FaiI YATR 5 cut(s) 267, 330, 347, 418, 423
FatI CATG 3 cut(s) 265, 328, 421
FbaI TGATCA 1 cut(s) 556
Fnu4HI GCNGC 4 cut(s) 83, 222, 225, 297
FokI GGATG 2 cut(s) 88, 336
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 4 cut(s) 83, 222, 225, 297
GluI GCNGC 4 cut(s) 83, 222, 225, 297
GsuI CTGGAG 1 cut(s) 586
HaeIII GGCC 2 cut(s) 233, 483
Hin1II CATG 3 cut(s) 269, 332, 425
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 2 cut(s) 188, 524
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 3 cut(s) 171, 288, 465
Hpy188III TCNNGA 2 cut(s) 329, 560
Hpy8I GTNNAC 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 89
HpyF10VI GCNNNNNNNGC 1 cut(s) 230
HpyF3I CTNAG 2 cut(s) 168, 534
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 3 cut(s) 269, 332, 425
Ksp22I TGATCA 1 cut(s) 556
Kzo9I GATC 2 cut(s) 379, 556
LmnI GCTCC 4 cut(s) 198, 211, 293, 497
Lsp1109I GCAGC 4 cut(s) 94, 208, 236, 283
LweI GCATC 2 cut(s) 69, 322
MabI ACCWGGT 1 cut(s) 355
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 3 cut(s) 250, 424, 546
MalI GATC 2 cut(s) 381, 558
MboI GATC 2 cut(s) 379, 556
MboII GAAGA 3 cut(s) 50, 118, 456
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 2 cut(s) 33, 162
MnlI CCTC 5 cut(s) 222, 284, 400, 403, 508
MseI TTAA 3 cut(s) 65, 194, 522
MspR9I CCNGG 2 cut(s) 357, 453
MunI CAATTG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 2 cut(s) 357, 453
MwoI GCNNNNNNNGC 1 cut(s) 230
NdeII GATC 2 cut(s) 379, 556
NlaIII CATG 3 cut(s) 269, 332, 425
NlaIV GGNNCC 2 cut(s) 118, 207
NspI RCATGY 1 cut(s) 269
PagI TCATGA 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 326
PctI GAATGC 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 458
PkrI GCNGC 4 cut(s) 84, 223, 226, 298
Psp124BI GAGCTC 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 355, 451
PspGI CCWGG 2 cut(s) 355, 451
PspN4I GGNNCC 2 cut(s) 118, 207
PspPI GGNCC 1 cut(s) 481
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 379
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
SacI GAGCTC 1 cut(s) 502
SaqAI TTAA 3 cut(s) 65, 194, 522
SatI GCNGC 4 cut(s) 83, 222, 225, 297
Sau3AI GATC 2 cut(s) 379, 556
Sau96I GGNCC 1 cut(s) 481
ScrFI CCNGG 2 cut(s) 357, 453
SduI GDGCHC 1 cut(s) 502
SexAI ACCWGGT 1 cut(s) 355
SfaNI GCATC 2 cut(s) 69, 322
SfcI CTRYAG 1 cut(s) 222
Sse9I AATT 2 cut(s) 33, 162
SstI GAGCTC 1 cut(s) 502
StyD4I CCNGG 2 cut(s) 355, 451
TaiI ACGT 1 cut(s) 92
TaqI TCGA 2 cut(s) 279, 502
TasI AATT 2 cut(s) 33, 162
Tru1I TTAA 3 cut(s) 65, 194, 522
Tru9I TTAA 3 cut(s) 65, 194, 522
TscAI CASTG 1 cut(s) 147
TseI GCWGC 4 cut(s) 82, 221, 224, 296
TspRI CASTG 1 cut(s) 147
Van91I CCANNNNNTGG 1 cut(s) 458
XceI RCATGY 1 cut(s) 269
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.