MD05G1271800.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007159
Physical Location & Seq
Reverse (-)
40710216 .. 40714798
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1271800.v1.1.491

Sequence Viewer

Length: 3150 bp
ATGGGGCAGCTCCCTTCGCAGGATATTTTGGCGCTGCTCGAGTTCAAGAAGGGTGTCAAGCATGACCCCACTGGCTATGTCCTTAATTCTTGGAATGATGAGTCCATTGACTTTGATGGCTGCCCATCTTCTTGGAACGGTGTTGTGTGTAATGGCGGAAATGTTGCTGGGGTTGTCCTTGATAACTTGAGTCTCTCTGCGGATGTGGACTTGAGTGTGTTTGCGAATCTAACGAAGCTTGTGAGACTTTCCATGGCCAACAACTCGATAATGGGGAAGTTTCCCGACAACATAGCTGACTTCAAAAGCCTTGAGTTCCTTGACCTGTCCAACAATTTATTTTCTTCACCTTTACTACCTGGGATTGGTAGGTTAGGGAGCTTAAGGAATCTCTCACTAGGTGGCAACAACTTCTCCGGTTCTATTCCAGATTCAATTTCAGGGCTTTCCTCAGTCCAGTTATTGGACTTGAGTCGAAACTCCTTGTCCGGGCCCCTGCCGGCATCATTGACAGAGCTGCCTAAACTGGTACATCTAAATTTGTCTTTAAATGGCTTTACCAAGAAAATACCCAAGGGGTTTGAGTTAATTTCAAGTCTCGATGTGCTTGACTTGCATGGGAACATGCTCGATGGGCATATCGATCCGGAATTTTTGATGCTATCTGAGGTCACTCATGTTGATTTGAGTGGCAATATGTTTGTGAGTTCCAGTTCACAACAGCAGAAGTTTCTACCCCGCCTTTCTGAGACTATTAAGTACTTGAATCTTAGCCACAACCAGCTCACTGGGTCACTGGTAAGCGGTGGTGAGCTACAGATGTTTGAAAACCTGAAGGTGTTGGATCTCAGCTACAACCAGCTGTCAGGGGAATTGCCTGGCTTTAATTTTGCCTATGATCTCCAAGTCCTCAAACTCAGCAACAACAGATTTACCGGAGATATTCCTAATGGTCTGTTAAAAGGAGATTCTCTTGTCGTAACTGAACTAGATTTGAGTGGCAACAATCTCACAGGACCAATAGATATGATCACATCGACCAATCTGTGCTTCCTCAATCTGTCCTCAAATGGGCTTACTGGTGAGTTACCATTGCTGACCGGAAGTTGTGCCGTGCTTGATCTATCGAATAACAAATTCGAAGGAAACTTAACCAGGATGGTGAAATGGGGGAACATCGAATATCTCGATCTGAGTCAAAATCATTTGGCAGGACCCATCCCTGATGTAACCCCACAGTTTTTGCGTTTAAATTATCTTAACCTCTCCCATAACACTCTTAGTAGTTCTATAGCAAGTGTTATAACACAATATCCAAAGATTAGCGTCTTTGATCTCAGTTCCAATCAGCTCAATGGAACGGTTCTAGCTGAATTGCTAACAATGCCCACTTTGCAAGAACTGCACCTTCGTGATAATTTACTCACCGGCTCGATTAACATCTCATCTCCTTTGCCCAGTGAGTCAAATCTTCAAGTTGTTGATCTTTCCCAGAACCATCTTAGTGGCTATTTTCCTGATCATTTTGGTTCATTGAAGGGGCTTAAAATGTTCAATATTGCTAGAAATAATTTTTCTGGCTCTCTTCCAACTTCCTTCACCAACATCAGCACTTTAAGCTCTTTAGACATATCCCAGAATCATTTTACAGGTCCTTTGCCAAACAACTTGCCTACTAGTCTTGAAAGCTTTAATGCTTCATACAATGATCTTTCCGGGGATGTCCCAGAAAATCTGAGAAAGTTTCCAAAATCTTCCTTCTATCCAGGGAATGCACGTTTGCTTTTTCCAAATGGTCCTCCGGGATCAAACAGTTCTGGAAATGAAAATTCAAAGAGGAGACCAATCAGTGCAATGGTCAAAGTAATCATTATAGTTTCATGTGTGGTTGCTGTCTTCATTCTTCTTCTGCTTGCTATCTTCGTACACTACATCCGTATGTCAAGAAGAATCCCATCAGAGCATACCACAAGCAAAAAAGACATCCACAGGCGCACTCAACCAAACCAATCTGGTGATCGTGGAACAGGCACGGGTGGTGCTTTGGTTGTTTCAGCTGAGGATCTCATGGCTTCACAAAAAGGATCATCATCCGAAACTGTCAGCCCCGATAAAAAGGTTGCAGCTGTAGCTGGTTTCTCCCCTTCAAAGCACAACCATTTCTCTTGGTCGCCAGACTCTGGAGAGTCGGTCACTACTGAAAACCTTGCAAGACTGGACGTGAGGTCACCGGATAAATTGTTTGGGGAGCTGCATTTTCTTGATGATACAATTGCATTAACCCCGGAGGAGCTGTCAAGGGCTCCTGCTGAAGTTCTAGGAAGAAGTAGCCATGGGACTTCTTACAGGGCAACACTTGATAATGGGTTGTTCTTGACGGTTAAGTGGTTGAGAGAAGGGGTGGCGAAACAGAAAAAGGAGTTTGCCAAGGAGGCTAAAAAGTTCGCGAACATGAGGCATCCAAATGTTGTAGGTTTGAGAGGGTACTATTGGGGTCCCACACAGCATGAGAAGCTCATTCTCTCCGATTATATCTCACCGGGAAGTCTTGCAAGCTTCCTTTATGATCGGCCAGGAAGAAAAGGTCCACCATTGACCTGGGCCCAAAGGCTCAAAATTGCAGTTGACGTCGCACGTGGCCTGAACTATCTCCATTTTGACCGTGCTGTTCCACACGGTAACCTCAAAGCATCAAATATTTTGTTGGACGGGCCAGACCTGAATGCCCGTGTTGCTGATTACTGCCTACACCGCCTCATGACACAAGCTGGCACCATCGAACAGATTCTTGATGCTGGTGTGTTGGGTTACCGTGCACCAGAGTTGGCTGCTTCCAAGAAGCCTCTTCCCTCCTTCAAGTCAGATATTTACGCCTTTGGAGTGATAATGCTGGAACTCCTAACTGGAAGGTGCGCGGGCGATGTGATTTCAGGTGAAGGAAGTGGGGTTGATTTGACAGATTGGGTTCGGCTGAGGGTGGCAGAAGGGCGCGGCTCTGAATGTTTTGACGCTGCATTGGTGCCGGAAATGGGAATGCCAGCAGCGGAGAAGGGAACGAAGGAGGTCCTTGGAATAGCGTTACGATGTCTAAGAAGTGTTGGGGAGAGGCCAGGTATTAAGAACATATATGAGGATCTCTCCTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1050

Amino Acids

113.87

Weight (kDa)

6.08

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 6 - 51 1.9e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 97 - 258 2.3e-10 Leucine-rich repeat region
LRR_8 PF13855 127 - 184 6.8e-08 Leucine rich repeat
LRR_8 PF13855 489 - 549 2.5e-09 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 771 - 969 1.2e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 771 - 970 3.2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1304
AatII GACGTC 1 cut(s) 2631
AccB1I GGYRCC 2 cut(s) 2771, 3019
AccB7I CCANNNNNTGG 2 cut(s) 463, 2180
AccII CGCG 3 cut(s) 2447, 2915, 2991
AccIII TCCGGA 1 cut(s) 646
AciI CCGC 8 cut(s) 156, 200, 739, 804, 2752, 2915, 2991, 3044
AclWI GGATC 6 cut(s) 638, 852, 1813, 2070, 2092, 3141
AcoI YGGCCR 2 cut(s) 255, 2570
AcsI RAATTY 4 cut(s) 538, 650, 1136, 1828
AcuI CTGAAG 2 cut(s) 854, 2331
AcvI CACGTG 1 cut(s) 2636
AcyI GRCGYC 1 cut(s) 2628
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 4 cut(s) 531, 761, 1926, 2486
AfiI CCNNNNNNNGG 7 cut(s) 19, 365, 463, 489, 1071, 2180, 3029
AflII CTTAAG 1 cut(s) 382
AhdI GACNNNNNGTC 1 cut(s) 2224
AhlI ACTAGT 1 cut(s) 1676
AjiI CACGTC 1 cut(s) 2221
AjnI CCWGG 7 cut(s) 358, 877, 1154, 1765, 2572, 2597, 3109
AjuI GAANNNNNNNTTGG 2 cut(s) 1034, 1066
AleI CACNNNNGTG 1 cut(s) 1410
Alw21I GWGCWC 1 cut(s) 2818
Alw26I GTCTC 5 cut(s) 197, 238, 602, 743, 1834
Alw44I GTGCAC 1 cut(s) 2814
AlwI GGATC 6 cut(s) 638, 852, 1813, 2070, 2092, 3141
AlwNI CAGNNNCTG 1 cut(s) 2180
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 646
AoxI GGCC 7 cut(s) 255, 491, 2570, 2601, 2638, 2711, 3107
ApaI GGGCCC 2 cut(s) 495, 2605
ApaLI GTGCAC 1 cut(s) 2814
ApeKI GCWGC 9 cut(s) 7, 34, 120, 517, 2123, 2251, 2828, 3011, 3041
ApoI RAATTY 4 cut(s) 538, 650, 1136, 1828
Asp700I GAANNNNTTC 1 cut(s) 2784
AspLEI GCGC 4 cut(s) 34, 1995, 2915, 2991
AsuC2I CCSGG 5 cut(s) 490, 1717, 1803, 2285, 2541
AsuII TTCGAA 1 cut(s) 1140
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 7 cut(s) 1016, 1214, 1652, 1796, 2495, 2585, 3064
BaeGI GKGCMC 3 cut(s) 495, 2605, 2818
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 2 cut(s) 2771, 3019
BanII GRGCYC 3 cut(s) 495, 2305, 2605
BbrPI CACGTG 1 cut(s) 2636
BbsI GAAGAC 1 cut(s) 1888
Bbv12I GWGCWC 1 cut(s) 2818
BbvCI CCTCAGC 2 cut(s) 2058, 2972
BbvI GCAGC 9 cut(s) 19, 21, 107, 504, 2135, 2238, 2815, 2998, 3053
BccI CCATC 8 cut(s) 110, 133, 626, 1153, 1226, 1506, 1963, 2783
BceAI ACGGC 1 cut(s) 1097
BciT130I CCWGG 7 cut(s) 360, 879, 1156, 1767, 2574, 2599, 3111
BclI TGATCA 2 cut(s) 1029, 1519
BcnI CCSGG 5 cut(s) 490, 1717, 1803, 2285, 2541
BcoDI GTCTC 5 cut(s) 197, 238, 602, 743, 1834
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 6 cut(s) 398, 989, 1367, 1563, 1677, 2318
BfmI CTRYAG 3 cut(s) 815, 1290, 2127
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 382
BglI GCCNNNNNGGC 1 cut(s) 2432
BmcAI AGTACT 1 cut(s) 761
Bme18I GGWCC 7 cut(s) 1016, 1214, 1652, 1796, 2495, 2585, 3064
BmeRI GACNNNNNGTC 1 cut(s) 2224
BmeT110I CYCGRG 1 cut(s) 38
BmgBI CACGTC 1 cut(s) 2221
BmiI GGNNCC 9 cut(s) 493, 494, 1216, 2304, 2496, 2497, 2603, 2773, 3021
BmrI ACTGGG 2 cut(s) 798, 1452
BmsI GCATC 5 cut(s) 512, 648, 2467, 2699, 2782
BmuI ACTGGG 2 cut(s) 798, 1452
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 1888
BplI GAGNNNNNCTC 1 cut(s) 3122
BpmI CTGGAG 1 cut(s) 2202
Bpu10I CCTNAGC 2 cut(s) 2058, 2972
Bpu14I TTCGAA 1 cut(s) 1140
BpuEI CTTGAG 4 cut(s) 208, 232, 332, 490
BpuMI CCSGG 5 cut(s) 490, 1717, 1803, 2285, 2541
Bsa29I ATCGAT 1 cut(s) 642
BsaAI YACGTR 1 cut(s) 2636
BsaBI GATNNNNATC 2 cut(s) 1439, 2089
BsaHI GRCGYC 1 cut(s) 2628
BsaI GGTCTC 1 cut(s) 1834
BsaWI WCCGGW 5 cut(s) 416, 646, 935, 1100, 2230
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 2175, 2205, 2279, 2309
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 365, 463, 489, 1071, 2180, 3029
Bse118I RCCGGY 2 cut(s) 499, 1427
Bse3DI GCAATG 2 cut(s) 1091, 1860
Bse8I GATNNNNATC 2 cut(s) 1439, 2089
BseAI TCCGGA 1 cut(s) 646
BseBI CCWGG 7 cut(s) 360, 879, 1156, 1767, 2574, 2599, 3111
BseCI ATCGAT 1 cut(s) 642
BseGI GGATG 8 cut(s) 208, 1164, 1218, 1726, 1932, 1983, 2090, 2458
BseJI GATNNNNATC 2 cut(s) 1439, 2089
BseLI CCNNNNNNNGG 7 cut(s) 19, 365, 463, 489, 1071, 2180, 3029
BseMI GCAATG 2 cut(s) 1091, 1860
BseRI GAGGAG 3 cut(s) 1852, 2303, 3130
BseSI GKGCMC 3 cut(s) 495, 2605, 2818
BseXI GCAGC 9 cut(s) 19, 21, 107, 504, 2135, 2238, 2815, 2998, 3053
BseYI CCCAGC 1 cut(s) 167
BsgI GTGCAG 1 cut(s) 1388
Bsh1236I CGCG 3 cut(s) 2447, 2915, 2991
BshFI GGCC 7 cut(s) 257, 493, 2572, 2603, 2640, 2713, 3109
BshNI GGYRCC 2 cut(s) 2771, 3019
BshVI ATCGAT 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 2818
BsiHKCI CYCGRG 1 cut(s) 38
BslFI GGGAC 3 cut(s) 1709, 2350, 2481
BslI CCNNNNNNNGG 7 cut(s) 19, 365, 463, 489, 1071, 2180, 3029
BsmAI GTCTC 5 cut(s) 197, 238, 602, 743, 1834
BsmFI GGGAC 3 cut(s) 1709, 2350, 2481
BsmI GAATGC 3 cut(s) 1777, 2728, 3039
BsnI GGCC 7 cut(s) 257, 493, 2572, 2603, 2640, 2713, 3109
Bso31I GGTCTC 1 cut(s) 1834
BsoBI CYCGRG 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 1140
Bsp120I GGGCCC 2 cut(s) 491, 2601
Bsp1286I GDGCHC 4 cut(s) 495, 2305, 2605, 2818
Bsp13I TCCGGA 1 cut(s) 646
Bsp19I CCATGG 2 cut(s) 252, 2332
Bsp68I TCGCGA 1 cut(s) 2447
BspACI CCGC 8 cut(s) 156, 200, 739, 804, 2752, 2915, 2991, 3044
BspANI GGCC 7 cut(s) 257, 493, 2572, 2603, 2640, 2713, 3109
BspDI ATCGAT 1 cut(s) 642
BspEI TCCGGA 1 cut(s) 646
BspFNI CGCG 3 cut(s) 2447, 2915, 2991
BspHI TCATGA 1 cut(s) 2757
BspLI GGNNCC 9 cut(s) 493, 494, 1216, 2304, 2496, 2497, 2603, 2773, 3021
BspPI GGATC 6 cut(s) 638, 852, 1813, 2070, 2092, 3141
BspT104I TTCGAA 1 cut(s) 1140
BspT107I GGYRCC 2 cut(s) 2771, 3019
BspTI CTTAAG 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 1834
BsrDI GCAATG 2 cut(s) 1091, 1860
BsrFI RCCGGY 2 cut(s) 499, 1427
BssAI RCCGGY 2 cut(s) 499, 1427
BssNI GRCGYC 1 cut(s) 2628
BssT1I CCWWGG 5 cut(s) 252, 573, 2332, 2427, 3067
Bst2UI CCWGG 7 cut(s) 360, 879, 1156, 1767, 2574, 2599, 3111
Bst4CI ACNGT 9 cut(s) 140, 1239, 1363, 1814, 2101, 2380, 2663, 2678, 2813
Bst6I CTCTTC 2 cut(s) 1590, 2850
BstACI GRCGYC 1 cut(s) 2628
BstAFI CTTAAG 1 cut(s) 382
BstAPI GCANNNNNTGC 1 cut(s) 1402
BstBAI YACGTR 1 cut(s) 2636
BstBI TTCGAA 1 cut(s) 1140
BstC8I GCNNGC 6 cut(s) 501, 1914, 2554, 2770, 2917, 3039
BstDSI CCRYGG 2 cut(s) 252, 2332
BstEII GGTNACC 3 cut(s) 2226, 2678, 2807
BstF5I GGATG 8 cut(s) 208, 1164, 1218, 1726, 1932, 1983, 2090, 2458
BstFNI CGCG 3 cut(s) 2447, 2915, 2991
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 4 cut(s) 34, 1995, 2915, 2991
BstMAI GTCTC 5 cut(s) 197, 238, 602, 743, 1834
BstNI CCWGG 7 cut(s) 360, 879, 1156, 1767, 2574, 2599, 3111
BstNSI RCATGY 1 cut(s) 628
BstPAI GACNNNNGTC 1 cut(s) 471
BstPI GGTNACC 3 cut(s) 2226, 2678, 2807
BstSFI CTRYAG 3 cut(s) 815, 1290, 2127
BstSLI GKGCMC 3 cut(s) 495, 2605, 2818
BstUI CGCG 3 cut(s) 2447, 2915, 2991
BstV1I GCAGC 9 cut(s) 19, 21, 107, 504, 2135, 2238, 2815, 2998, 3053
BstV2I GAAGAC 1 cut(s) 1888
BstX2I RGATCY 3 cut(s) 844, 2062, 3133
BstXI CCANNNNNNTGG 4 cut(s) 132, 788, 1505, 2598
BstYI RGATCY 3 cut(s) 844, 2062, 3133
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 7 cut(s) 257, 493, 2572, 2603, 2640, 2713, 3109
BsuTUI ATCGAT 1 cut(s) 642
BtgI CCRYGG 2 cut(s) 252, 2332
BtgZI GCGATG 1 cut(s) 2934
BtrI CACGTC 1 cut(s) 2221
BtsCI GGATG 8 cut(s) 208, 1164, 1218, 1726, 1932, 1983, 2090, 2458
BtsIMutI CAGTG 5 cut(s) 69, 786, 794, 1465, 1855
BtuMI TCGCGA 1 cut(s) 2447
Cac8I GCNNGC 6 cut(s) 501, 1914, 2554, 2770, 2917, 3039
CaiI CAGNNNCTG 1 cut(s) 2180
CciI TCATGA 1 cut(s) 2757
CfoI GCGC 4 cut(s) 34, 1995, 2915, 2991
Cfr10I RCCGGY 2 cut(s) 499, 1427
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 2 cut(s) 1315, 3017
Csp6I GTAC 4 cut(s) 530, 760, 1925, 2485
CspCI CAANNNNNGTGG 2 cut(s) 1224, 1259
CviQI GTAC 4 cut(s) 530, 760, 1925, 2485
DraI TTTAAA 2 cut(s) 549, 1251
DraIII CACNNNGTG 1 cut(s) 401
DriI GACNNNNNGTC 1 cut(s) 2224
EaeI YGGCCR 2 cut(s) 255, 2570
Eam1104I CTCTTC 2 cut(s) 1590, 2850
Eam1105I GACNNNNNGTC 1 cut(s) 2224
EarI CTCTTC 2 cut(s) 1590, 2850
EciI GGCGGA 1 cut(s) 171
Eco130I CCWWGG 5 cut(s) 252, 573, 2332, 2427, 3067
Eco24I GRGCYC 3 cut(s) 495, 2305, 2605
Eco31I GGTCTC 1 cut(s) 1834
Eco47I GGWCC 7 cut(s) 1016, 1214, 1652, 1796, 2495, 2585, 3064
Eco57I CTGAAG 2 cut(s) 854, 2331
Eco72I CACGTG 1 cut(s) 2636
Eco88I CYCGRG 1 cut(s) 38
Eco91I GGTNACC 3 cut(s) 2226, 2678, 2807
EcoO109I RGGNCCY 5 cut(s) 492, 1214, 1652, 2495, 3064
EcoO65I GGTNACC 3 cut(s) 2226, 2678, 2807
EcoRII CCWGG 7 cut(s) 358, 877, 1154, 1765, 2572, 2597, 3109
EcoT14I CCWWGG 5 cut(s) 252, 573, 2332, 2427, 3067
EcoT38I GRGCYC 3 cut(s) 495, 2305, 2605
ErhI CCWWGG 5 cut(s) 252, 573, 2332, 2427, 3067
FaqI GGGAC 3 cut(s) 1709, 2350, 2481
FauI CCCGC 2 cut(s) 746, 2908
FbaI TGATCA 2 cut(s) 1029, 1519
FokI GGATG 8 cut(s) 215, 1171, 1205, 1733, 1919, 1970, 2077, 2445
FriOI GRGCYC 3 cut(s) 495, 2305, 2605
FspBI CTAG 6 cut(s) 398, 989, 1367, 1563, 1677, 2318
GlaI GCGC 4 cut(s) 33, 1994, 2914, 2990
GsaI CCCAGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 2202
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 7 cut(s) 257, 493, 2572, 2603, 2640, 2713, 3109
HgaI GACGC 2 cut(s) 1315, 3017
HhaI GCGC 4 cut(s) 34, 1995, 2915, 2991
Hin1I GRCGYC 1 cut(s) 2628
Hin6I GCGC 4 cut(s) 32, 1993, 2913, 2989
HinP1I GCGC 4 cut(s) 32, 1993, 2913, 2989
HincII GTYRAC 1 cut(s) 2626
HindII GTYRAC 1 cut(s) 2626
HindIII AAGCTT 3 cut(s) 236, 1687, 2554
Hpy166II GTNNAC 6 cut(s) 208, 716, 1927, 2588, 2626, 2816
Hpy188I TCNGA 9 cut(s) 667, 748, 1194, 1737, 1960, 2095, 2527, 2863, 2998
Hpy8I GTNNAC 6 cut(s) 208, 716, 1927, 2588, 2626, 2816
Hpy99I CGWCG 1 cut(s) 2633
HpyCH4III ACNGT 9 cut(s) 140, 1239, 1363, 1814, 2101, 2380, 2663, 2678, 2813
HpyCH4IV ACGT 4 cut(s) 1777, 2220, 2628, 2635
HpySE526I ACGT 4 cut(s) 1777, 2220, 2628, 2635
Hsp92I GRCGYC 1 cut(s) 2628
HspAI GCGC 4 cut(s) 32, 1993, 2913, 2989
KflI GGGWCCC 1 cut(s) 2495
Kpn2I TCCGGA 1 cut(s) 646
KroI GCCGGC 1 cut(s) 499
KroNI GCCGGC 1 cut(s) 501
Ksp22I TGATCA 2 cut(s) 1029, 1519
LmnI GCTCC 5 cut(s) 15, 378, 2248, 2290, 2308
Lsp1109I GCAGC 9 cut(s) 19, 21, 107, 504, 2135, 2238, 2815, 2998, 3053
LweI GCATC 5 cut(s) 512, 648, 2467, 2699, 2782
MaeI CTAG 6 cut(s) 398, 989, 1367, 1563, 1677, 2318
MaeII ACGT 4 cut(s) 1777, 2220, 2628, 2635
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 3 cut(s) 844, 2062, 3133
MhlI GDGCHC 4 cut(s) 495, 2305, 2605, 2818
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 7 cut(s) 110, 199, 481, 1204, 1472, 2171, 2195
MmeI TCCRAC 4 cut(s) 354, 822, 1613, 2685
Mox20I TGGCCA 1 cut(s) 257
MroI TCCGGA 1 cut(s) 646
MroNI GCCGGC 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 2784
MscI TGGCCA 1 cut(s) 257
MslI CAYNNNNRTG 4 cut(s) 1410, 1503, 1937, 2463
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 4 cut(s) 862, 2057, 2126, 3044
MspCI CTTAAG 1 cut(s) 382
MunI CAATTG 1 cut(s) 2271
Mva1269I GAATGC 3 cut(s) 1777, 2728, 3039
MvaI CCWGG 7 cut(s) 360, 879, 1156, 1767, 2574, 2599, 3111
MvnI CGCG 3 cut(s) 2447, 2915, 2991
NaeI GCCGGC 1 cut(s) 501
NciI CCSGG 5 cut(s) 490, 1717, 1803, 2285, 2541
NcoI CCATGG 2 cut(s) 252, 2332
NgoMIV GCCGGC 1 cut(s) 499
NlaIV GGNNCC 9 cut(s) 493, 494, 1216, 2304, 2496, 2497, 2603, 2773, 3021
NmuCI GTSAC 4 cut(s) 670, 792, 2191, 2226
NruI TCGCGA 1 cut(s) 2447
NspI RCATGY 1 cut(s) 628
NspV TTCGAA 1 cut(s) 1140
OliI CACNNNNGTG 1 cut(s) 1410
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 2757
PcsI WCGNNNNNNNCGW 1 cut(s) 1185
PctI GAATGC 3 cut(s) 1777, 2728, 3039
PdiI GCCGGC 1 cut(s) 501
PdmI GAANNNNTTC 1 cut(s) 2784
PfeI GAWTC 8 cut(s) 226, 388, 431, 766, 968, 1639, 1950, 2785
PflMI CCANNNNNTGG 2 cut(s) 463, 2180
PfoI TCCNGGA 1 cut(s) 1801
PleI GAGTC 7 cut(s) 109, 198, 480, 1203, 1471, 2171, 2194
PmaCI CACGTG 1 cut(s) 2636
PmlI CACGTG 1 cut(s) 2636
PpsI GAGTC 7 cut(s) 109, 198, 480, 1203, 1471, 2171, 2194
Ppu21I YACGTR 1 cut(s) 2636
PpuMI RGGWCCY 4 cut(s) 1214, 1652, 2495, 3064
PshAI GACNNNNGTC 1 cut(s) 471
PsiI TTATAA 1 cut(s) 1304
Psp5II RGGWCCY 4 cut(s) 1214, 1652, 2495, 3064
Psp6I CCWGG 7 cut(s) 358, 877, 1154, 1765, 2572, 2597, 3109
PspCI CACGTG 1 cut(s) 2636
PspEI GGTNACC 3 cut(s) 2226, 2678, 2807
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 7 cut(s) 358, 877, 1154, 1765, 2572, 2597, 3109
PspN4I GGNNCC 9 cut(s) 493, 494, 1216, 2304, 2496, 2497, 2603, 2773, 3021
PspOMI GGGCCC 2 cut(s) 491, 2601
PspPPI RGGWCCY 4 cut(s) 1214, 1652, 2495, 3064
PspXI VCTCGAGB 1 cut(s) 38
PstNI CAGNNNCTG 1 cut(s) 2180
PsuI RGATCY 3 cut(s) 844, 2062, 3133
PvuII CAGCTG 3 cut(s) 862, 2057, 2126
RruI TCGCGA 1 cut(s) 2447
RsaI GTAC 4 cut(s) 531, 761, 1926, 2486
RsaNI GTAC 4 cut(s) 530, 760, 1925, 2485
RseI CAYNNNNRTG 4 cut(s) 1410, 1503, 1937, 2463
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 7 cut(s) 110, 199, 481, 1204, 1472, 2171, 2195
SduI GDGCHC 4 cut(s) 495, 2305, 2605, 2818
SfaNI GCATC 5 cut(s) 512, 648, 2467, 2699, 2782
SfcI CTRYAG 3 cut(s) 815, 1290, 2127
Sfr274I CTCGAG 1 cut(s) 38
SfuI TTCGAA 1 cut(s) 1140
SinI GGWCC 7 cut(s) 1016, 1214, 1652, 1796, 2495, 2585, 3064
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 4 cut(s) 1410, 1503, 1937, 2463
SmlI CTYRAG 6 cut(s) 38, 187, 211, 311, 382, 469
SmoI CTYRAG 6 cut(s) 38, 187, 211, 311, 382, 469
SpeI ACTAGT 1 cut(s) 1676
SsiI CCGC 8 cut(s) 156, 200, 739, 804, 2752, 2915, 2991, 3044
SspI AATATT 2 cut(s) 1558, 2698
SspMI CTAG 6 cut(s) 398, 989, 1367, 1563, 1677, 2318
StyI CCWWGG 5 cut(s) 252, 573, 2332, 2427, 3067
TaaI ACNGT 9 cut(s) 140, 1239, 1363, 1814, 2101, 2380, 2663, 2678, 2813
TaiI ACGT 4 cut(s) 1780, 2223, 2631, 2638
TaqII GACCGA 1 cut(s) 2179
TatI WGTACW 1 cut(s) 759
TauI GCSGC 1 cut(s) 2994
TfiI GAWTC 8 cut(s) 226, 388, 431, 766, 968, 1639, 1950, 2785
TscAI CASTG 5 cut(s) 76, 793, 801, 1465, 1855
TseFI GTSAC 4 cut(s) 670, 792, 2191, 2226
TseI GCWGC 9 cut(s) 7, 34, 120, 517, 2123, 2251, 2828, 3011, 3041
Tsp45I GTSAC 4 cut(s) 670, 792, 2191, 2226
TspDTI ATGAA 5 cut(s) 1521, 1689, 1839, 1869, 1888
TspGWI ACGGA 1 cut(s) 1925
TspRI CASTG 5 cut(s) 76, 793, 801, 1465, 1855
Van91I CCANNNNNTGG 2 cut(s) 463, 2180
Vha464I CTTAAG 1 cut(s) 382
VneI GTGCAC 1 cut(s) 2814
VpaK11BI GGWCC 7 cut(s) 1016, 1214, 1652, 1796, 2495, 2585, 3064
XapI RAATTY 4 cut(s) 538, 650, 1136, 1828
XceI RCATGY 1 cut(s) 628
XcmI CCANNNNNNNNNTGG 1 cut(s) 2595
XhoI CTCGAG 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 2784
XspI CTAG 6 cut(s) 398, 989, 1367, 1563, 1677, 2318
ZraI GACGTC 1 cut(s) 2629
ZrmI AGTACT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.