pycom05g25410

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007159
Physical Location & Seq
Reverse (-)
26768372 .. 26771964
3593 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25410.1

Sequence Viewer

Length: 3192 bp
ATGCTCCGTCGGCTTTTTGTGGTGTCCCTTCTGTTTATTTCTGCCATGGGGCAGCTCCCTTCGCAGGATATTTTGGCGCTGCTCGAGTTCAAGAAGGGTGTCAAGCATGACCCCACTGGCTATGTCCTTAATTCTTGGAATGATGAGTCCATTGACTTTGATGGCTGCCCATCTTCTTGGAACGGTGTCGTGTGTAATGGCGGAAATGTTGCTGGGGTTGTCCTTGATAATTTGAGTCTCTCTGCGGATGTGGACTTGAGTGTGTTTGTGAATCTAACGAAGCTTGTGAGACTTTCCATGGCCAACAACTCGATAATGGGAAAGTTTCCCGACAACATAGCTGACTTCAAAAGTCTTGAGTTCCTTGACCTGTCCAACAATTTATTTTCTTCACCTTTACCACCTGGGATTGGTAGGTTAGGGAGCTTAAGGAATCTCTCACTTGGTGGCAACAACTTCTCGGGTTCTATTCCAGATTCAATTTCAGGGCTTTCCTCAGTCCAGTTATTGGACTTGAGTCGAAACTCCTTGTCCGGGCCCCTGCCGGCATCATTGACAGAGCTGCCTAAACTGGTACATCTAAATTTGTCTTTAAATGGCTTTACCAAGAAAATACCTAAGGGGTTTGAGTTAATTTCAAGTCTCGATGTGCTTGACTTGCATGGGAACATGCTCGATGGGCATATGAATCTGGAGTTTTTGATGCTATCTGAGGTCACTCATGTTGATTTGAGTGGCAATATGTTTGTTAGTTCCAGCTCACAACAGCAGAAGTTTCTACCCCGCCTTTCTGAGACTATTAAGTACTTGAATCTTAGCCACAACCAGCTCACTGGGTCACTGGTAAGCGGTGGTGAGCTACAGATGTTTGAAAACCTGAAGGTGTTGGATCTCAGCTACAACCAGCTGTCAGGGGATTTGCCAGGCTTTAATTTTGCCTATGATCTCCAAGTACTCAAACTCAGCAACAACAGATTTACCGGAGATATTCCTAATGGTCTATTAAAAGGAGATTCTCTTGTTGTAACTGAACTAGATTTGAGTGGCAACAATCTCACAGGACGAATAGATATGATCACATCGACCAATCTGCGCTTCCTCAATCTGTCCTCAAATGGGATTACAGGTGAGTTACCGTTGCTGACTGGAAGTTGTGCCGTACTTGATCTATCGAATAACAAATTCGAAGGAAACTTAACCAGGATGGTGAAATGGGGAAACATAGAATATCTCGATCTGAGTCAAAATCATTTTACAGGACCCATCCCTGATGTAACCCCACAGTTTTTGCGTTTAAATTATCTTAACCTCTCCCATAACACTCTTAGTAGTTCTATAGCAAGTGTTATAACACAATATCCAAAGATTAGTGTCCTTGATCTCAGTTCCAATCAGCTCGATGGAACGGTTCTAGCTGAATTGCTAACAATGCCCACTTTGCAAGAACTGCACCTTCGTGATAATTTACTCACCGGCTCGATTAACATCTCATCTCCTTTGCCCAGTGAGTCAAATCTTCAAGTTGTTGATCTTTCCCAGAACCATCTTAGTGGCTATTTTCCTGATCATTTTGGTTCATTGAAGGGGCTTAAAATGTTCAATATTGCTAGAAATAATTTTTCTGGCTCTCTTCCAACTTCCGTCACCAACATCAGCACTTTAAGCTCATTAGACATTTCCCAGAATCATTTTACAGGTCCTTTGCCAAACAACTTGCCTACTAGTCTTGAAAGCTTTAATGCTTCATACAATGATCTTTCTGGGGATGTCCCGGAAAATCTGAGAAAGTTTCCAAAATCTTCCTTCTATCCAGGGAATGCACGTTTGCTTTTTCCAAATGGTCCTCCGGGATCAAACAGTTCTGGAAATGAAAATTCAAAGAGGAGACCAATCAGTACAATGGTCAAAGTAATCATTATAGTTTCATGTGTGGTTGCTGTCTTCATTCTTCTTCTGCTTGCTATCTTCGTACACTACATCCGCATGTCAAGAAGAATCCCATCAGAGCATACCACAAGCAAAGACATCCACAGTCGCACTCAACCAAACCAATCTGGTGTTCATGGAACAGGCACGGGTGGTGCTTTGGTCGTTTCAACTGCGGATCTCATGGCTTCACAAAAAGGATCATCATCCGAAATTGTCAGCCCCGATAAAAAAGTTGCAGCTGTAGCTGGTTTCTCCCCTTCAAAGCACAGCCATTTCTCTTGGTCCCCAGACTCTGGAGAGTCGGTCACTACTGAAAACCTCGCAAGACTGGATGTGAGGTCACCGGATAAATTGGTTGGTGAGCTGCATTTTCTTGATGATACAATTGCATTAACCCCGGAGGAGCTGTCAAGGGCACCTGCTGAAGTTCTAGGAAGAAGTAGCCATGGGACTTCTTACAGGGCAACACTTGATACTGGGTTGTTCTTGACGGTTAAGTGGTTGAGAGAAGGGGTGGCGAAACAGAAAAAGGAGTTTGCCAAGGAGGCTAAAAAGTTTGCGAACATGAGGCATCCAAATGTTGTAGGTTTGAGAGGGTACTACTGGGGCCCCACACAGCATGAGAAGCTCATTCTTTCCGATTATATCTCACCGGGAAGTCTTGCAAGCTTTCTTTACGATCGACCAGGAAGAAAAGATCCACCATTGACCTGGGCCCAAAGGCTCAAAATTGCAGTTGACGTTGCACGTGGCCTGAACTATCTCCATTTTGACCGTGCTGTTCCACACGGTAACCTCAAAGCATCAAATATTTTGTTGGACGGGCCAGACCTGAATGCCCGTGTTGCTGATTACTGCCTCCACCGCCTCATGACACAAGCTGGCACCATCGAACAGATTCTCGATGCTGGTGTCTTGGGTTACCGTGCACCAGAGTTGGCTGCTTCCAAGAAGCCTCTTCCCTCCTTCAAGTCAGATATTTACGCCTTTGGAGTGATAATGCTGGAACTCCTAACTGGAAGGTGTGCGGGCGATGTGATTTCAGGTGAAGGAAATGGGGTTGATTTGACAGATTGGGTTCAGCTGAGGGTAGCAGAAGGGCGCGGCTCTGAATGTTTTGACGCTGCATTGGTGCTGGAAATGGGAGTGCCAGCAGCAGAGAAGGGAACGAAGGAGGTCCTTGGAATAGCGTTACGATGTTTAAGAACTGTTGGGGAGAGGCCAGGTATTAAGAACATATATGAGGATCTCTCCTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1064

Amino Acids

115.49

Weight (kDa)

6.16

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 66 1.9e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 137 - 274 1.6e-07 Leucine-rich repeat region
LRR_8 PF13855 142 - 199 9e-08 Leucine rich repeat
LRR_8 PF13855 504 - 564 5.2e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 785 - 982 1.4e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 785 - 984 2.2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1349
AarI CACCTGC 1 cut(s) 2356
Acc36I ACCTGC 1 cut(s) 2356
AccB1I GGYRCC 2 cut(s) 2344, 2813
AccB7I CCANNNNNTGG 2 cut(s) 508, 2222
AccII CGCG 1 cut(s) 3033
AciI CCGC 9 cut(s) 201, 245, 784, 849, 1981, 2102, 2794, 2957, 3033
AclWI GGATC 6 cut(s) 897, 1858, 2112, 2134, 2621, 3183
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 583, 1181, 1873
AcuI CTGAAG 2 cut(s) 899, 2373
AcvI CACGTG 1 cut(s) 2678
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 7 cut(s) 576, 806, 954, 1161, 1897, 1971, 2528
AfiI CCNNNNNNNGG 6 cut(s) 64, 410, 508, 534, 1116, 2222
AflII CTTAAG 1 cut(s) 427
AhlI ACTAGT 1 cut(s) 1721
AjnI CCWGG 7 cut(s) 403, 922, 1199, 1810, 2614, 2639, 3151
AjuI GAANNNNNNNTTGG 4 cut(s) 1079, 1111, 2043, 2075
AleI CACNNNNGTG 1 cut(s) 1455
Alw21I GWGCWC 1 cut(s) 2860
Alw26I GTCTC 5 cut(s) 242, 283, 647, 788, 1879
Alw44I GTGCAC 1 cut(s) 2856
AlwI GGATC 6 cut(s) 897, 1858, 2112, 2134, 2621, 3183
AlwNI CAGNNNCTG 1 cut(s) 2222
Ama87I CYCGRG 2 cut(s) 83, 460
AoxI GGCC 7 cut(s) 300, 536, 2536, 2643, 2680, 2753, 3149
ApaI GGGCCC 3 cut(s) 540, 2540, 2647
ApaLI GTGCAC 1 cut(s) 2856
ApeKI GCWGC 9 cut(s) 52, 79, 165, 562, 2165, 2293, 2870, 3053, 3083
ApoI RAATTY 3 cut(s) 583, 1181, 1873
Asp700I GAANNNNTTC 1 cut(s) 2826
AspLEI GCGC 3 cut(s) 79, 1095, 3033
AsuC2I CCSGG 5 cut(s) 535, 1772, 1848, 2327, 2583
AsuII TTCGAA 1 cut(s) 1185
AvaI CYCGRG 2 cut(s) 83, 460
AvaII GGWCC 5 cut(s) 1259, 1697, 1841, 2211, 3106
AxyI CCTNAGG 1 cut(s) 618
BaeGI GKGCMC 5 cut(s) 540, 2347, 2540, 2647, 2860
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 2 cut(s) 2344, 2813
BanII GRGCYC 3 cut(s) 540, 2540, 2647
BbrPI CACGTG 1 cut(s) 2678
BbsI GAAGAC 1 cut(s) 1933
Bbv12I GWGCWC 1 cut(s) 2860
BbvCI CCTCAGC 1 cut(s) 3014
BbvI GCAGC 9 cut(s) 64, 66, 152, 549, 2177, 2280, 2857, 3040, 3095
BccI CCATC 9 cut(s) 155, 178, 671, 1198, 1271, 1394, 1551, 2008, 2825
BceAI ACGGC 1 cut(s) 1142
BcgI CGANNNNNNTGC 2 cut(s) 1072, 1106
BciT130I CCWGG 7 cut(s) 405, 924, 1201, 1812, 2616, 2641, 3153
BclI TGATCA 2 cut(s) 1074, 1564
BcnI CCSGG 5 cut(s) 535, 1772, 1848, 2327, 2583
BcoDI GTCTC 5 cut(s) 242, 283, 647, 788, 1879
BcuI ACTAGT 1 cut(s) 1721
BfaI CTAG 5 cut(s) 1034, 1412, 1608, 1722, 2360
BfmI CTRYAG 3 cut(s) 860, 1335, 2169
BfoI RGCGCY 1 cut(s) 80
BfrI CTTAAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 2356
BglI GCCNNNNNGGC 1 cut(s) 2474
BmcAI AGTACT 2 cut(s) 806, 954
Bme18I GGWCC 5 cut(s) 1259, 1697, 1841, 2211, 3106
BmeT110I CYCGRG 2 cut(s) 83, 460
BmrI ACTGGG 4 cut(s) 843, 1497, 2415, 2542
BmsI GCATC 5 cut(s) 557, 693, 2509, 2741, 2824
BmuI ACTGGG 4 cut(s) 843, 1497, 2415, 2542
BoxI GACNNNNGTC 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 1933
BplI GAGNNNNNCTC 1 cut(s) 3164
BpmI CTGGAG 2 cut(s) 713, 2244
Bpu10I CCTNAGC 1 cut(s) 3014
Bpu14I TTCGAA 1 cut(s) 1185
BpuEI CTTGAG 3 cut(s) 277, 377, 535
BpuMI CCSGG 5 cut(s) 535, 1772, 1848, 2327, 2583
BsaAI YACGTR 1 cut(s) 2678
BsaBI GATNNNNATC 2 cut(s) 1484, 2131
BsaI GGTCTC 1 cut(s) 1879
BsaJI CCNNGG 9 cut(s) 45, 297, 404, 1811, 2325, 2374, 2469, 2640, 3109
BsaWI WCCGGW 2 cut(s) 980, 2272
BsaXI ACNNNNNCTCC 4 cut(s) 2217, 2247, 2321, 2351
Bsc4I CCNNNNNNNGG 6 cut(s) 64, 410, 508, 534, 1116, 2222
Bse118I RCCGGY 2 cut(s) 544, 1472
Bse21I CCTNAGG 1 cut(s) 618
Bse8I GATNNNNATC 2 cut(s) 1484, 2131
BseBI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2616, 2641, 3153
BseDI CCNNGG 9 cut(s) 45, 297, 404, 1811, 2325, 2374, 2469, 2640, 3109
BseGI GGATG 9 cut(s) 253, 1209, 1263, 1771, 1977, 2025, 2132, 2266, 2500
BseJI GATNNNNATC 2 cut(s) 1484, 2131
BseLI CCNNNNNNNGG 6 cut(s) 64, 410, 508, 534, 1116, 2222
BseMII CTCAG 9 cut(s) 510, 702, 783, 907, 976, 1229, 1396, 1772, 3005
BseRI GAGGAG 3 cut(s) 1897, 2345, 3172
BseSI GKGCMC 5 cut(s) 540, 2347, 2540, 2647, 2860
BseXI GCAGC 9 cut(s) 64, 66, 152, 549, 2177, 2280, 2857, 3040, 3095
BseYI CCCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 1433
Bsh1236I CGCG 1 cut(s) 3033
Bsh1285I CGRYCG 1 cut(s) 2611
BshFI GGCC 7 cut(s) 302, 538, 2538, 2645, 2682, 2755, 3151
BshNI GGYRCC 2 cut(s) 2344, 2813
BsiEI CGRYCG 1 cut(s) 2611
BsiHKAI GWGCWC 1 cut(s) 2860
BsiHKCI CYCGRG 2 cut(s) 83, 460
BsiSI CCGG 9 cut(s) 534, 545, 981, 1473, 1772, 1847, 2273, 2327, 2582
BslFI GGGAC 4 cut(s) 10, 1754, 2197, 2392
BslI CCNNNNNNNGG 6 cut(s) 64, 410, 508, 534, 1116, 2222
BsmAI GTCTC 5 cut(s) 242, 283, 647, 788, 1879
BsmFI GGGAC 4 cut(s) 10, 1754, 2197, 2392
BsmI GAATGC 2 cut(s) 1822, 2770
BsnI GGCC 7 cut(s) 302, 538, 2538, 2645, 2682, 2755, 3151
Bso31I GGTCTC 1 cut(s) 1879
BsoBI CYCGRG 2 cut(s) 83, 460
Bsp119I TTCGAA 1 cut(s) 1185
Bsp120I GGGCCC 3 cut(s) 536, 2536, 2643
Bsp1286I GDGCHC 5 cut(s) 540, 2347, 2540, 2647, 2860
Bsp19I CCATGG 3 cut(s) 45, 297, 2374
BspACI CCGC 9 cut(s) 201, 245, 784, 849, 1981, 2102, 2794, 2957, 3033
BspANI GGCC 7 cut(s) 302, 538, 2538, 2645, 2682, 2755, 3151
BspCNI CTCAG 9 cut(s) 509, 703, 784, 906, 975, 1230, 1395, 1773, 3006
BspFNI CGCG 1 cut(s) 3033
BspHI TCATGA 1 cut(s) 2799
BspMI ACCTGC 1 cut(s) 2356
BspPI GGATC 6 cut(s) 897, 1858, 2112, 2134, 2621, 3183
BspT104I TTCGAA 1 cut(s) 1185
BspT107I GGYRCC 2 cut(s) 2344, 2813
BspTI CTTAAG 1 cut(s) 427
BspTNI GGTCTC 1 cut(s) 1879
BsrFI RCCGGY 2 cut(s) 544, 1472
BssAI RCCGGY 2 cut(s) 544, 1472
BssECI CCNNGG 9 cut(s) 45, 297, 404, 1811, 2325, 2374, 2469, 2640, 3109
BssT1I CCWWGG 5 cut(s) 45, 297, 2374, 2469, 3109
Bst2UI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2616, 2641, 3153
Bst6I CTCTTC 2 cut(s) 1635, 2892
BstAFI CTTAAG 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 1447
BstBAI YACGTR 1 cut(s) 2678
BstBI TTCGAA 1 cut(s) 1185
BstC8I GCNNGC 6 cut(s) 546, 1959, 2596, 2812, 2959, 3081
BstDSI CCRYGG 3 cut(s) 45, 297, 2374
BstEII GGTNACC 3 cut(s) 2268, 2720, 2849
BstF5I GGATG 9 cut(s) 253, 1209, 1263, 1771, 1977, 2025, 2132, 2266, 2500
BstFNI CGCG 1 cut(s) 3033
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 3 cut(s) 79, 1095, 3033
BstMAI GTCTC 5 cut(s) 242, 283, 647, 788, 1879
BstMCI CGRYCG 1 cut(s) 2611
BstNI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2616, 2641, 3153
BstNSI RCATGY 2 cut(s) 673, 1987
BstPAI GACNNNNGTC 1 cut(s) 516
BstPI GGTNACC 3 cut(s) 2268, 2720, 2849
BstSFI CTRYAG 3 cut(s) 860, 1335, 2169
BstSLI GKGCMC 5 cut(s) 540, 2347, 2540, 2647, 2860
BstUI CGCG 1 cut(s) 3033
BstV1I GCAGC 9 cut(s) 64, 66, 152, 549, 2177, 2280, 2857, 3040, 3095
BstV2I GAAGAC 1 cut(s) 1933
BstX2I RGATCY 4 cut(s) 889, 2104, 2626, 3175
BstXI CCANNNNNNTGG 4 cut(s) 177, 833, 1550, 2640
BstYI RGATCY 4 cut(s) 889, 2104, 2626, 3175
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 7 cut(s) 302, 538, 2538, 2645, 2682, 2755, 3151
BtgI CCRYGG 3 cut(s) 45, 297, 2374
BtgZI GCGATG 1 cut(s) 2976
BtsCI GGATG 9 cut(s) 253, 1209, 1263, 1771, 1977, 2025, 2132, 2266, 2500
BtsIMutI CAGTG 4 cut(s) 114, 831, 839, 1510
BveI ACCTGC 1 cut(s) 2356
Cac8I GCNNGC 6 cut(s) 546, 1959, 2596, 2812, 2959, 3081
CaiI CAGNNNCTG 1 cut(s) 2222
CciI TCATGA 1 cut(s) 2799
CfoI GCGC 3 cut(s) 79, 1095, 3033
Cfr10I RCCGGY 2 cut(s) 544, 1472
CseI GACGC 1 cut(s) 3059
Csp6I GTAC 7 cut(s) 575, 805, 953, 1160, 1896, 1970, 2527
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviQI GTAC 7 cut(s) 575, 805, 953, 1160, 1896, 1970, 2527
DraI TTTAAA 2 cut(s) 594, 1296
DraIII CACNNNGTG 1 cut(s) 446
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 1635, 2892
EarI CTCTTC 2 cut(s) 1635, 2892
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 5 cut(s) 45, 297, 2374, 2469, 3109
Eco24I GRGCYC 3 cut(s) 540, 2540, 2647
Eco31I GGTCTC 1 cut(s) 1879
Eco47I GGWCC 5 cut(s) 1259, 1697, 1841, 2211, 3106
Eco57I CTGAAG 2 cut(s) 899, 2373
Eco72I CACGTG 1 cut(s) 2678
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 2 cut(s) 83, 460
Eco91I GGTNACC 3 cut(s) 2268, 2720, 2849
EcoO109I RGGNCCY 6 cut(s) 537, 1259, 1697, 2536, 2537, 3106
EcoO65I GGTNACC 3 cut(s) 2268, 2720, 2849
EcoRII CCWGG 7 cut(s) 403, 922, 1199, 1810, 2614, 2639, 3151
EcoT14I CCWWGG 5 cut(s) 45, 297, 2374, 2469, 3109
EcoT38I GRGCYC 3 cut(s) 540, 2540, 2647
ErhI CCWWGG 5 cut(s) 45, 297, 2374, 2469, 3109
FaqI GGGAC 4 cut(s) 10, 1754, 2197, 2392
FauI CCCGC 2 cut(s) 791, 2950
FauNDI CATATG 1 cut(s) 684
FbaI TGATCA 2 cut(s) 1074, 1564
FokI GGATG 9 cut(s) 260, 1216, 1250, 1778, 1964, 2012, 2119, 2273, 2487
FriOI GRGCYC 3 cut(s) 540, 2540, 2647
FspBI CTAG 5 cut(s) 1034, 1412, 1608, 1722, 2360
GlaI GCGC 3 cut(s) 78, 1094, 3032
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 2 cut(s) 713, 2244
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 7 cut(s) 302, 538, 2538, 2645, 2682, 2755, 3151
HapII CCGG 9 cut(s) 534, 545, 981, 1473, 1772, 1847, 2273, 2327, 2582
HgaI GACGC 1 cut(s) 3059
HhaI GCGC 3 cut(s) 79, 1095, 3033
Hin6I GCGC 3 cut(s) 77, 1093, 3031
HinP1I GCGC 3 cut(s) 77, 1093, 3031
HincII GTYRAC 1 cut(s) 2668
HindII GTYRAC 1 cut(s) 2668
HindIII AAGCTT 3 cut(s) 281, 1732, 2596
HpaII CCGG 9 cut(s) 534, 545, 981, 1473, 1772, 1847, 2273, 2327, 2582
Hpy166II GTNNAC 4 cut(s) 253, 1972, 2668, 2858
Hpy188I TCNGA 9 cut(s) 712, 793, 1239, 1782, 2005, 2137, 2569, 2905, 3040
Hpy8I GTNNAC 4 cut(s) 253, 1972, 2668, 2858
Hpy99I CGWCG 1 cut(s) 12
HpyCH4IV ACGT 3 cut(s) 1822, 2670, 2677
HpySE526I ACGT 3 cut(s) 1822, 2670, 2677
HspAI GCGC 3 cut(s) 77, 1093, 3031
KroI GCCGGC 1 cut(s) 544
KroNI GCCGGC 1 cut(s) 546
Ksp22I TGATCA 2 cut(s) 1074, 1564
LmnI GCTCC 4 cut(s) 9, 60, 423, 2332
Lsp1109I GCAGC 9 cut(s) 64, 66, 152, 549, 2177, 2280, 2857, 3040, 3095
LweI GCATC 5 cut(s) 557, 693, 2509, 2741, 2824
MaeI CTAG 5 cut(s) 1034, 1412, 1608, 1722, 2360
MaeII ACGT 3 cut(s) 1822, 2670, 2677
MfeI CAATTG 1 cut(s) 2313
MflI RGATCY 4 cut(s) 889, 2104, 2626, 3175
MhlI GDGCHC 5 cut(s) 540, 2347, 2540, 2647, 2860
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 7 cut(s) 155, 244, 526, 1249, 1517, 2213, 2237
MmeI TCCRAC 4 cut(s) 399, 867, 1658, 2727
Mox20I TGGCCA 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 544
MroXI GAANNNNTTC 1 cut(s) 2826
MscI TGGCCA 1 cut(s) 302
MslI CAYNNNNRTG 4 cut(s) 1455, 1548, 1982, 2505
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 3 cut(s) 907, 2168, 3013
MspCI CTTAAG 1 cut(s) 427
MspI CCGG 9 cut(s) 534, 545, 981, 1473, 1772, 1847, 2273, 2327, 2582
MunI CAATTG 1 cut(s) 2313
Mva1269I GAATGC 2 cut(s) 1822, 2770
MvaI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2616, 2641, 3153
MvnI CGCG 1 cut(s) 3033
NaeI GCCGGC 1 cut(s) 546
NciI CCSGG 5 cut(s) 535, 1772, 1848, 2327, 2583
NcoI CCATGG 3 cut(s) 45, 297, 2374
NdeI CATATG 1 cut(s) 684
NgoMIV GCCGGC 1 cut(s) 544
NmuCI GTSAC 5 cut(s) 715, 837, 1642, 2233, 2268
NspI RCATGY 2 cut(s) 673, 1987
NspV TTCGAA 1 cut(s) 1185
OliI CACNNNNGTG 1 cut(s) 1455
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 2799
PaqCI CACCTGC 1 cut(s) 2356
PctI GAATGC 2 cut(s) 1822, 2770
PdiI GCCGGC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 2826
PfeI GAWTC 9 cut(s) 271, 433, 476, 688, 811, 1013, 1684, 1995, 2827
PflMI CCANNNNNTGG 2 cut(s) 508, 2222
PfoI TCCNGGA 2 cut(s) 1770, 1846
Ple19I CGATCG 1 cut(s) 2611
PleI GAGTC 7 cut(s) 154, 243, 525, 1248, 1516, 2213, 2236
PmaCI CACGTG 1 cut(s) 2678
PmlI CACGTG 1 cut(s) 2678
PpsI GAGTC 7 cut(s) 154, 243, 525, 1248, 1516, 2213, 2236
Ppu21I YACGTR 1 cut(s) 2678
PpuMI RGGWCCY 3 cut(s) 1259, 1697, 3106
PshAI GACNNNNGTC 1 cut(s) 516
PsiI TTATAA 1 cut(s) 1349
Psp5II RGGWCCY 3 cut(s) 1259, 1697, 3106
Psp6I CCWGG 7 cut(s) 403, 922, 1199, 1810, 2614, 2639, 3151
PspCI CACGTG 1 cut(s) 2678
PspEI GGTNACC 3 cut(s) 2268, 2720, 2849
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 7 cut(s) 403, 922, 1199, 1810, 2614, 2639, 3151
PspOMI GGGCCC 3 cut(s) 536, 2536, 2643
PspPPI RGGWCCY 3 cut(s) 1259, 1697, 3106
PspXI VCTCGAGB 1 cut(s) 83
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 4 cut(s) 889, 2104, 2626, 3175
PvuI CGATCG 1 cut(s) 2611
PvuII CAGCTG 3 cut(s) 907, 2168, 3013
RsaI GTAC 7 cut(s) 576, 806, 954, 1161, 1897, 1971, 2528
RsaNI GTAC 7 cut(s) 575, 805, 953, 1160, 1896, 1970, 2527
RseI CAYNNNNRTG 4 cut(s) 1455, 1548, 1982, 2505
ScaI AGTACT 2 cut(s) 806, 954
SchI GAGTC 7 cut(s) 155, 244, 526, 1249, 1517, 2213, 2237
SduI GDGCHC 5 cut(s) 540, 2347, 2540, 2647, 2860
SfaNI GCATC 5 cut(s) 557, 693, 2509, 2741, 2824
SfcI CTRYAG 3 cut(s) 860, 1335, 2169
Sfr274I CTCGAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 1185
SinI GGWCC 5 cut(s) 1259, 1697, 1841, 2211, 3106
SlaI CTCGAG 1 cut(s) 83
SmiMI CAYNNNNRTG 4 cut(s) 1455, 1548, 1982, 2505
SmlI CTYRAG 5 cut(s) 83, 256, 356, 427, 514
SmoI CTYRAG 5 cut(s) 83, 256, 356, 427, 514
SpeI ACTAGT 1 cut(s) 1721
SsiI CCGC 9 cut(s) 201, 245, 784, 849, 1981, 2102, 2794, 2957, 3033
SspI AATATT 2 cut(s) 1603, 2740
SspMI CTAG 5 cut(s) 1034, 1412, 1608, 1722, 2360
StyI CCWWGG 5 cut(s) 45, 297, 2374, 2469, 3109
TaiI ACGT 3 cut(s) 1825, 2673, 2680
TaqII GACCGA 1 cut(s) 2221
TatI WGTACW 3 cut(s) 804, 952, 1895
TauI GCSGC 1 cut(s) 3036
TfiI GAWTC 9 cut(s) 271, 433, 476, 688, 811, 1013, 1684, 1995, 2827
TscAI CASTG 4 cut(s) 121, 838, 846, 1510
TseFI GTSAC 5 cut(s) 715, 837, 1642, 2233, 2268
TseI GCWGC 9 cut(s) 52, 79, 165, 562, 2165, 2293, 2870, 3053, 3083
Tsp45I GTSAC 5 cut(s) 715, 837, 1642, 2233, 2268
TspDTI ATGAA 7 cut(s) 701, 1566, 1734, 1884, 1914, 1933, 2051
TspGWI ACGGA 1 cut(s) 1630
TspRI CASTG 4 cut(s) 121, 838, 846, 1510
Van91I CCANNNNNTGG 2 cut(s) 508, 2222
Vha464I CTTAAG 1 cut(s) 427
VneI GTGCAC 1 cut(s) 2856
VpaK11BI GGWCC 5 cut(s) 1259, 1697, 1841, 2211, 3106
XapI RAATTY 3 cut(s) 583, 1181, 1873
XceI RCATGY 2 cut(s) 673, 1987
XcmI CCANNNNNNNNNTGG 1 cut(s) 2637
XhoI CTCGAG 1 cut(s) 83
XmnI GAANNNNTTC 1 cut(s) 2826
XspI CTAG 5 cut(s) 1034, 1412, 1608, 1722, 2360
ZrmI AGTACT 2 cut(s) 806, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.