RchiOBHm_Chr5g0016431

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007159
Physical Location & Seq
Forward (+)
11297216 .. 11302317
5102 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29680

Sequence Viewer

Length: 3204 bp
ATGAGTATGAGGCTGGGTGGGATTTTAGTGCTGTCCCTATTTTTCCTTTCTGCAATGGGACAGCTTCCTTCGCAGGATATTTTGGCGCTGCTCGCGTTCAAGAAGGGTGTCAAGCATGATCCTACTGGTTTTGTCGTCAATTCTTGGAATGATGAGTCCATTGACTTCAATGGTTGTCCTTCTTCTTGGAATGGGGTTATATGTAATGGTGGAAATGTCGCTGGAGTTGTCCTTGATAATTTGAGTCTCTCTGCGGATGTGGACTTGAGTGTGTTTTCGAATCTAACCAAGCTTCTGAAACTTTCCATGGCCAACAATTCGATATCTGGGAAAATTCCTGACAATATTGCAGACTTCAACAGCCTCCAGTTTCTTGATCTCTCCAACAATTTATTTGCTTCATCTTTACCACCTGGAATTGGTAAGTTGGGGAGCTTAAGGAACCTCTCATTAGCTGGCAATAACTTCTCGGGCTCCATTCCAGATTCCATTTCAGGGCTTTCCTCAATCCAGTCGTTGGACTTAAGTCGCAACTCCTTTTCAGGGCCGATGCCGTCCGCATTGACAAAGCTGTCCAGTCTGGTATCTCTAAATCTTTCTTTGAATGGCTTTACCAAGAGCTTACCTAAGGGGTTTGAGCTAATGTCAAGTCTAGATGTGCTCGACTTGCATGGAAATATGCTTGATGGTCCTCTGGATAAGGCATTTCTGATGGAAGCAACTGCCACGCATGTTGATTTTAGTGGCAATATGTTTACAAGTTCCAGTTCACAGCAGCAGATGTTTCTACCTCGTCTTTCCGAGAGTATCAAGTACTTGAATCTTTCCCACAACCAGCTTACTGGGTCACTGGTAGGTGGCAGTGAGCTTCAGATATTTGAGGACTTGAAGGTGTTGGATCTCAGCTACAACCAGCTGTCAGGAGAATTGCCTGGATTTAATTTTGTCTATGACCTCCAGGTCCTCAAACTTAGCAACAACAGATTTACTGGAGTTGTTCCCAATGGCCTAATAAAAGGAGATTCTCTGGTTTTAAGTGAACTAGATCTGAGTGGCAACAATCTCTCAGGACCAATAACTATGGTGACATCAACAACTCTGAGCATCCTTAATCTCTCCTCGAATGGGCTTACTGGTAAGTTGCCATTGCTGACTGGAAGTTGTGCTGTACTTGATCTATCCAAGAACAAATTAGAGGGAAATTTAACCAGGATGGTGAAATGGGGGAACATTGAATACCTTGACCTGAGTCAAAATCTTTTGACAGGACCCATCCCTGATGTCACTCCACAGTTTTTGCGTTTAAATTATCTCAATCTTTCCCATAATTCTCTTAGTAGTTCCATAGCAAGTGTTATAACACAGTATCCAAAAATTAGTGTCCTTGATCTCAGTTCTAACCAGTTAGATGGCACAGTTCTAGCTGAGTTGCTAAGCATGCCTACTCTGCAAGAGCTCCACCTTGAAAATAATTTACTCACTGGGTCTATCAACATTTCATCTCCTTTGTTCAACCAATCCAATCTTCAGGTTCTTGATCTTTCTCAGAACCGGCTGAGTGGTTATTTTCCTGATCAATTTGGTTCACTGAATGGGCTTAAAGTGCTCAATATTGGGAGAAATAATCTTTCTGGCTCTCTGCCAACTTCCATATCTGACATGAGCACACTAAGCTCATTAGACATATCGCAGAATCATTTCACAGGTCCTCTTCCAAACAACTTGCCAAACAGTCTTGTATTCTTTAATGCTTCATATAATGATCTTTCTGGGGATGTCCCAGAAAATCTGAGAAAGTTTCCGAGTTCTTCCTTCTATCCAGGGAATACGCGCTTGCGGTTTCCAAGTGGTGGTCCTCCTGGATCAAACAATTCCGAATCTGAACATTCAAAGAGGAAACCATTCAGCACACTTGTCAAAGTTATCATTATAGTTTCATGTGTGGTTGCTGTTTTCATTCTTCTTCTGCTTGGTATCTTCATTCATTACATCTGCATGTCAAGGAGAACCCCATCAGAGAATACTGCAAACAAAGACATCCACAAACGTGCTCCACCAAACCCCTCAGGTGCTCGAGGAGCAGAGAGTGGTGGTGCTTTGGTTGTTTCAGCTGGGGATCTTGTGGCTTCACGGAAAGGATCGTCTTCTGAAATTATCAGCCCTGGTGAAAAACTCGCAGCTGTAAGTGACTTCTCCCCTTCAAAGAACAGCCATTTCTCTTGGTCACCAGAGTCTGGAGATTCCTACACTGCAGAAAACCTTGCCAGACTGGACGTGAGATCACCGGATAGATTGGTTGGTGAGCTTCATTTTCTTGATGAAACAATTGCATTAACACCTGAGGAGCTTTCAAGGGCTCCTGCTGAAGTTTTGGGAAGAAGCAGCCATGGCACATCTTATAAGGCAACACTTGATAACGGGCTGTTCTTGACAGTTAAGTGGTTGAGAGAAGGGGTGGCAAAGCAGAAAAAGGAGTTTGCCAAGGAGGCTAAGAAATTTGCCAATATGAGGCATCCAAATGTTGTGGGTTTGAGAGGGTACTATTGGGGTCCTACACAGCATGAGAAGCTTATTCTTTCAGATTATATCTCACCTGGAAGTCTTGCAAGCTTTCTTTATGATCGACCAGGAAGAAAAGGTCCACCATTGACCTGGGCCCAAAGGCTCAAAATTGCAGTTGATGTTGCACGTGGCCTGAACTATCTCCATTTTGATCGTGCTGTTCCACACGGTAACCTCAAAGCAACAAATATTCTGTTGGATGGGTCTGATCTGAATGCCCGAGTTGCTGATTACTGCCTCCACCGTCTCATGACACAAGCTGGCACCATTGAACAGATTCTTGATGCTGGTGTCTTAGGTTACCGCGCCCCAGAGCTGGCTGCTTCCAAGAAGCCACTGCCCTCCTTCAAGTCCGATGTTTACGCCTTTGGAGTGATATTGTTGGAACTTTTAACTGGAAGGTGCGCTGGTGATGTGATCTCTGGTGAAGGAGGCGGGGCAGATTTGACAGAATGGGTGCGGTTGAGGGTAGCAGAAGGTCGTGGGTCAGATTGTTTTGATGCTGCATTGGTGACTGAGATGGGAAATCCAGCAGCAGAGAAGGGAATGAAGGAAGTCCTTGGAATATCATTACGGTGTATACGTTCTGTCTCTGAGAGGCCAGGTATTAAGACTATATATGAGGATCTATCTTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1067

Amino Acids

114.87

Weight (kDa)

6.18

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 69 2.5e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 86 - 131 8.3e-06 Leucine rich repeat
LRR_14 PF23598 140 - 224 9.5e-08 Leucine-rich repeat region
LRR_8 PF13855 507 - 567 2.5e-11 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 789 - 987 4e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 789 - 988 1.2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1358, 2400
AasI GACNNNNNNGTC 2 cut(s) 571, 959
AccB1I GGYRCC 1 cut(s) 2825
AccB7I CCANNNNNTGG 3 cut(s) 517, 1850, 2234
AccI GTMKAC 1 cut(s) 3142
AccII CGCG 3 cut(s) 95, 1831, 2868
AciI CCGC 6 cut(s) 254, 558, 1837, 2866, 2997, 3022
AclWI GGATC 6 cut(s) 113, 906, 1870, 2124, 2146, 3195
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 3 cut(s) 333, 1201, 2495
AcuI CTGAAG 3 cut(s) 854, 1511, 2385
AcvI CACGTG 1 cut(s) 2690
AfaI GTAC 3 cut(s) 815, 1170, 2540
AfiI CCNNNNNNNGG 9 cut(s) 419, 495, 517, 543, 1125, 1850, 2234, 2508, 2878
AflII CTTAAG 2 cut(s) 436, 523
AjiI CACGTC 1 cut(s) 2275
AjuI GAANNNNNNNTTGG 2 cut(s) 1509, 1541
AleI CACNNNNGTG 1 cut(s) 2046
Alw21I GWGCWC 6 cut(s) 663, 1458, 1608, 1667, 2053, 2074
Alw26I GTCTC 3 cut(s) 251, 2813, 3157
AlwI GGATC 6 cut(s) 113, 906, 1870, 2124, 2146, 3195
AlwNI CAGNNNCTG 1 cut(s) 2234
Ama87I CYCGRG 3 cut(s) 469, 2073, 2781
AoxI GGCC 6 cut(s) 309, 545, 1006, 2655, 2692, 3161
ApaI GGGCCC 1 cut(s) 2659
ApeKI GCWGC 7 cut(s) 88, 775, 2177, 2382, 2882, 3065, 3095
ApoI RAATTY 3 cut(s) 333, 1201, 2495
Asp700I GAANNNNTTC 3 cut(s) 1697, 1901, 2838
AspLEI GCGC 4 cut(s) 88, 1833, 2870, 2969
AsuII TTCGAA 1 cut(s) 278
AvaI CYCGRG 3 cut(s) 469, 2073, 2781
AvaII GGWCC 8 cut(s) 689, 961, 1070, 1268, 1706, 1853, 2549, 2639
AxyI CCTNAGG 3 cut(s) 627, 2065, 2340
BaeGI GKGCMC 1 cut(s) 2659
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 2825
BanII GRGCYC 4 cut(s) 476, 1458, 2359, 2659
BbrPI CACGTG 1 cut(s) 2690
BbsI GAAGAC 1 cut(s) 2136
Bbv12I GWGCWC 6 cut(s) 663, 1458, 1608, 1667, 2053, 2074
BbvI GCAGC 7 cut(s) 75, 787, 2189, 2394, 2869, 3052, 3107
BccI CCATC 8 cut(s) 680, 706, 1207, 1280, 1403, 2020, 2756, 3076
BceAI ACGGC 1 cut(s) 538
BciVI GTATCC 1 cut(s) 1377
BclI TGATCA 1 cut(s) 1573
BcoDI GTCTC 3 cut(s) 251, 2813, 3157
BfaI CTAG 3 cut(s) 653, 1043, 1421
BfmI CTRYAG 1 cut(s) 2250
BfoI RGCGCY 1 cut(s) 89
BfrI CTTAAG 2 cut(s) 436, 523
BfuI GTATCC 1 cut(s) 1377
BglI GCCNNNNNGGC 1 cut(s) 2486
BglII AGATCT 1 cut(s) 1045
BisI GCNGC 7 cut(s) 89, 776, 2178, 2383, 2883, 3066, 3096
BlpI GCTNAGC 1 cut(s) 1433
BlsI GCNGC 7 cut(s) 90, 777, 2179, 2384, 2884, 3067, 3097
BmcAI AGTACT 1 cut(s) 815
Bme18I GGWCC 8 cut(s) 689, 961, 1070, 1268, 1706, 1853, 2549, 2639
BmeT110I CYCGRG 3 cut(s) 469, 2073, 2781
BmgBI CACGTC 1 cut(s) 2275
BmiI GGNNCC 7 cut(s) 443, 475, 1270, 2358, 2550, 2657, 2827
BmrI ACTGGG 2 cut(s) 852, 1491
BmsI GCATC 5 cut(s) 540, 1113, 2521, 2836, 3052
BmuI ACTGGG 2 cut(s) 852, 1491
BoxI GACNNNNGTC 2 cut(s) 525, 1248
BpiI GAAGAC 1 cut(s) 2136
BpmI CTGGAG 5 cut(s) 243, 350, 941, 1011, 2256
Bpu1102I GCTNAGC 1 cut(s) 1433
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 286
BsaAI YACGTR 1 cut(s) 2690
BsaJI CCNNGG 7 cut(s) 306, 1820, 2161, 2386, 2481, 2652, 3121
BsaWI WCCGGW 1 cut(s) 2284
Bsc4I CCNNNNNNNGG 9 cut(s) 419, 495, 517, 543, 1125, 1850, 2234, 2508, 2878
Bse118I RCCGGY 1 cut(s) 1551
Bse21I CCTNAGG 3 cut(s) 627, 2065, 2340
Bse3DI GCAATG 2 cut(s) 60, 1145
BseDI CCNNGG 7 cut(s) 306, 1820, 2161, 2386, 2481, 2652, 3121
BseGI GGATG 8 cut(s) 262, 1104, 1218, 1272, 1780, 2037, 2512, 2767
BseLI CCNNNNNNNGG 9 cut(s) 419, 495, 517, 543, 1125, 1850, 2234, 2508, 2878
BseMI GCAATG 2 cut(s) 60, 1145
BseRI GAGGAG 3 cut(s) 1108, 2091, 2357
BseSI GKGCMC 1 cut(s) 2659
BseXI GCAGC 7 cut(s) 75, 787, 2189, 2394, 2869, 3052, 3107
BseYI CCCAGC 2 cut(s) 13, 2111
Bsh1236I CGCG 3 cut(s) 95, 1831, 2868
BshFI GGCC 6 cut(s) 311, 547, 1008, 2657, 2694, 3163
BshNI GGYRCC 1 cut(s) 2825
BsiHKAI GWGCWC 6 cut(s) 663, 1458, 1608, 1667, 2053, 2074
BsiHKCI CYCGRG 3 cut(s) 469, 2073, 2781
BsiSI CCGG 2 cut(s) 1552, 2285
BslFI GGGAC 3 cut(s) 19, 72, 1763
BslI CCNNNNNNNGG 9 cut(s) 419, 495, 517, 543, 1125, 1850, 2234, 2508, 2878
BsmAI GTCTC 3 cut(s) 251, 2813, 3157
BsmBI CGTCTC 1 cut(s) 2813
BsmFI GGGAC 3 cut(s) 19, 72, 1763
BsmI GAATGC 1 cut(s) 2782
BsnI GGCC 6 cut(s) 311, 547, 1008, 2657, 2694, 3163
BsoBI CYCGRG 3 cut(s) 469, 2073, 2781
Bsp119I TTCGAA 1 cut(s) 278
Bsp120I GGGCCC 1 cut(s) 2655
Bsp1286I GDGCHC 9 cut(s) 476, 663, 1458, 1608, 1667, 2053, 2074, 2359, 2659
Bsp1720I GCTNAGC 1 cut(s) 1433
Bsp19I CCATGG 2 cut(s) 306, 2386
BspACI CCGC 6 cut(s) 254, 558, 1837, 2866, 2997, 3022
BspANI GGCC 6 cut(s) 311, 547, 1008, 2657, 2694, 3163
BspFNI CGCG 3 cut(s) 95, 1831, 2868
BspHI TCATGA 1 cut(s) 2811
BspLI GGNNCC 7 cut(s) 443, 475, 1270, 2358, 2550, 2657, 2827
BspMAI CTGCAG 1 cut(s) 2254
BspPI GGATC 6 cut(s) 113, 906, 1870, 2124, 2146, 3195
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 2825
BspTI CTTAAG 2 cut(s) 436, 523
BsrDI GCAATG 2 cut(s) 60, 1145
BsrFI RCCGGY 1 cut(s) 1551
BssAI RCCGGY 1 cut(s) 1551
BssECI CCNNGG 7 cut(s) 306, 1820, 2161, 2386, 2481, 2652, 3121
BssNAI GTATAC 1 cut(s) 3143
BssT1I CCWWGG 4 cut(s) 306, 2386, 2481, 3121
Bst1107I GTATAC 1 cut(s) 3143
Bst4CI ACNGT 8 cut(s) 1293, 1365, 1417, 1733, 2434, 2732, 2807, 3138
Bst6I CTCTTC 1 cut(s) 1716
BstAFI CTTAAG 2 cut(s) 436, 523
BstBAI YACGTR 1 cut(s) 2690
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 7 cut(s) 93, 457, 1439, 1835, 2608, 2824, 2880
BstDSI CCRYGG 2 cut(s) 306, 2386
BstEII GGTNACC 3 cut(s) 2223, 2732, 2861
BstF5I GGATG 8 cut(s) 262, 1104, 1218, 1272, 1780, 2037, 2512, 2767
BstFNI CGCG 3 cut(s) 95, 1831, 2868
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 4 cut(s) 88, 1833, 2870, 2969
BstMAI GTCTC 3 cut(s) 251, 2813, 3157
BstNSI RCATGY 3 cut(s) 734, 1441, 1999
BstPAI GACNNNNGTC 2 cut(s) 525, 1248
BstPI GGTNACC 3 cut(s) 2223, 2732, 2861
BstSFI CTRYAG 1 cut(s) 2250
BstSLI GKGCMC 1 cut(s) 2659
BstUI CGCG 3 cut(s) 95, 1831, 2868
BstV1I GCAGC 7 cut(s) 75, 787, 2189, 2394, 2869, 3052, 3107
BstV2I GAAGAC 1 cut(s) 2136
BstX2I RGATCY 4 cut(s) 898, 1045, 2116, 3187
BstXI CCANNNNNNTGG 3 cut(s) 842, 1409, 2652
BstYI RGATCY 4 cut(s) 898, 1045, 2116, 3187
BstZ17I GTATAC 1 cut(s) 3143
Bsu36I CCTNAGG 3 cut(s) 627, 2065, 2340
BsuI GTATCC 1 cut(s) 1377
BsuRI GGCC 6 cut(s) 311, 547, 1008, 2657, 2694, 3163
BtgI CCRYGG 2 cut(s) 306, 2386
BtrI CACGTC 1 cut(s) 2275
BtsCI GGATG 8 cut(s) 262, 1104, 1218, 1272, 1780, 2037, 2512, 2767
BtsI GCAGTG 3 cut(s) 868, 2247, 2897
BtsIMutI CAGTG 6 cut(s) 848, 868, 1479, 1586, 2247, 2897
Cac8I GCNNGC 7 cut(s) 93, 457, 1439, 1835, 2608, 2824, 2880
CaiI CAGNNNCTG 1 cut(s) 2234
CciI TCATGA 1 cut(s) 2811
CfoI GCGC 4 cut(s) 88, 1833, 2870, 2969
Cfr10I RCCGGY 1 cut(s) 1551
Csp6I GTAC 3 cut(s) 814, 1169, 2539
CspCI CAANNNNNGTGG 6 cut(s) 715, 750, 1278, 1313, 2505, 2540
CviQI GTAC 3 cut(s) 814, 1169, 2539
DraI TTTAAA 1 cut(s) 1305
DrdI GACNNNNNNGTC 2 cut(s) 571, 959
DseDI GACNNNNNNGTC 2 cut(s) 571, 959
EaeI YGGCCR 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 1716
EarI CTCTTC 1 cut(s) 1716
Ecl136II GAGCTC 1 cut(s) 1456
Eco130I CCWWGG 4 cut(s) 306, 2386, 2481, 3121
Eco24I GRGCYC 4 cut(s) 476, 1458, 2359, 2659
Eco32I GATATC 1 cut(s) 324
Eco47I GGWCC 8 cut(s) 689, 961, 1070, 1268, 1706, 1853, 2549, 2639
Eco53kI GAGCTC 1 cut(s) 1456
Eco57I CTGAAG 3 cut(s) 854, 1511, 2385
Eco72I CACGTG 1 cut(s) 2690
Eco81I CCTNAGG 3 cut(s) 627, 2065, 2340
Eco88I CYCGRG 3 cut(s) 469, 2073, 2781
Eco91I GGTNACC 3 cut(s) 2223, 2732, 2861
EcoICRI GAGCTC 1 cut(s) 1456
EcoO109I RGGNCCY 4 cut(s) 961, 1268, 1706, 2549
EcoO65I GGTNACC 3 cut(s) 2223, 2732, 2861
EcoRV GATATC 1 cut(s) 324
EcoT14I CCWWGG 4 cut(s) 306, 2386, 2481, 3121
EcoT38I GRGCYC 4 cut(s) 476, 1458, 2359, 2659
ErhI CCWWGG 4 cut(s) 306, 2386, 2481, 3121
Esp3I CGTCTC 1 cut(s) 2813
FaqI GGGAC 3 cut(s) 19, 72, 1763
FauI CCCGC 1 cut(s) 2990
FbaI TGATCA 1 cut(s) 1573
FblI GTMKAC 1 cut(s) 3142
Fnu4HI GCNGC 7 cut(s) 89, 776, 2178, 2383, 2883, 3066, 3096
FokI GGATG 8 cut(s) 269, 1091, 1225, 1259, 1787, 2024, 2499, 2774
FriOI GRGCYC 4 cut(s) 476, 1458, 2359, 2659
Fsp4HI GCNGC 7 cut(s) 89, 776, 2178, 2383, 2883, 3066, 3096
FspBI CTAG 3 cut(s) 653, 1043, 1421
GlaI GCGC 4 cut(s) 87, 1832, 2869, 2968
GluI GCNGC 7 cut(s) 89, 776, 2178, 2383, 2883, 3066, 3096
GsaI CCCAGC 2 cut(s) 17, 2115
GsuI CTGGAG 5 cut(s) 243, 350, 941, 1011, 2256
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 6 cut(s) 311, 547, 1008, 2657, 2694, 3163
HapII CCGG 2 cut(s) 1552, 2285
HhaI GCGC 4 cut(s) 88, 1833, 2870, 2969
Hin6I GCGC 4 cut(s) 86, 1831, 2868, 2967
HinP1I GCGC 4 cut(s) 86, 1831, 2868, 2967
HindIII AAGCTT 3 cut(s) 290, 2567, 2608
HpaII CCGG 2 cut(s) 1552, 2285
Hpy166II GTNNAC 8 cut(s) 262, 756, 770, 1040, 1586, 2642, 2923, 3143
Hpy8I GTNNAC 8 cut(s) 262, 756, 770, 1040, 1586, 2642, 2923, 3143
HpyCH4III ACNGT 8 cut(s) 1293, 1365, 1417, 1733, 2434, 2732, 2807, 3138
HpyCH4IV ACGT 4 cut(s) 2047, 2274, 2689, 3145
HpySE526I ACGT 4 cut(s) 2047, 2274, 2689, 3145
HspAI GCGC 4 cut(s) 86, 1831, 2868, 2967
Ksp22I TGATCA 1 cut(s) 1573
LmnI GCTCC 7 cut(s) 432, 479, 1461, 2056, 2078, 2344, 2362
Lsp1109I GCAGC 7 cut(s) 75, 787, 2189, 2394, 2869, 3052, 3107
LweI GCATC 5 cut(s) 540, 1113, 2521, 2836, 3052
MaeI CTAG 3 cut(s) 653, 1043, 1421
MaeII ACGT 4 cut(s) 2047, 2274, 2689, 3145
MaeIII GTNAC 8 cut(s) 846, 1084, 1282, 2186, 2223, 2732, 2861, 3073
MfeI CAATTG 1 cut(s) 2325
MflI RGATCY 4 cut(s) 898, 1045, 2116, 3187
MhlI GDGCHC 9 cut(s) 476, 663, 1458, 1608, 1667, 2053, 2074, 2359, 2659
MlsI TGGCCA 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 4 cut(s) 164, 253, 1258, 2240
MmeI TCCRAC 5 cut(s) 408, 498, 876, 2739, 2925
Mox20I TGGCCA 1 cut(s) 311
MroXI GAANNNNTTC 3 cut(s) 1697, 1901, 2838
MscI TGGCCA 1 cut(s) 311
MslI CAYNNNNRTG 4 cut(s) 1994, 2046, 2517, 3136
Msp20I TGGCCA 1 cut(s) 311
MspA1I CMGCKG 3 cut(s) 916, 2111, 2180
MspCI CTTAAG 2 cut(s) 436, 523
MspI CCGG 2 cut(s) 1552, 2285
MunI CAATTG 1 cut(s) 2325
Mva1269I GAATGC 1 cut(s) 2782
MvnI CGCG 3 cut(s) 95, 1831, 2868
NcoI CCATGG 2 cut(s) 306, 2386
NlaIV GGNNCC 7 cut(s) 443, 475, 1270, 2358, 2550, 2657, 2827
NmuCI GTSAC 6 cut(s) 846, 1084, 1282, 2186, 2223, 3073
NspI RCATGY 3 cut(s) 734, 1441, 1999
NspV TTCGAA 1 cut(s) 278
OliI CACNNNNGTG 1 cut(s) 2046
PaeI GCATGC 1 cut(s) 1441
PaeR7I CTCGAG 1 cut(s) 2073
PagI TCATGA 1 cut(s) 2811
PcsI WCGNNNNNNNCGW 1 cut(s) 3142
PctI GAATGC 1 cut(s) 2782
PdmI GAANNNNTTC 3 cut(s) 1697, 1901, 2838
PfeI GAWTC 8 cut(s) 280, 485, 820, 1022, 1693, 1877, 2240, 2839
PflMI CCANNNNNTGG 3 cut(s) 517, 1850, 2234
PfoI TCCNGGA 1 cut(s) 1858
PkrI GCNGC 7 cut(s) 90, 777, 2179, 2384, 2884, 3067, 3097
PleI GAGTC 4 cut(s) 163, 252, 1257, 2239
PmaCI CACGTG 1 cut(s) 2690
PmlI CACGTG 1 cut(s) 2690
PpsI GAGTC 4 cut(s) 163, 252, 1257, 2239
Ppu21I YACGTR 1 cut(s) 2690
PpuMI RGGWCCY 4 cut(s) 961, 1268, 1706, 2549
PshAI GACNNNNGTC 2 cut(s) 525, 1248
PsiI TTATAA 2 cut(s) 1358, 2400
Psp124BI GAGCTC 1 cut(s) 1458
Psp5II RGGWCCY 4 cut(s) 961, 1268, 1706, 2549
PspCI CACGTG 1 cut(s) 2690
PspEI GGTNACC 3 cut(s) 2223, 2732, 2861
PspFI CCCAGC 2 cut(s) 13, 2111
PspN4I GGNNCC 7 cut(s) 443, 475, 1270, 2358, 2550, 2657, 2827
PspOMI GGGCCC 1 cut(s) 2655
PspPPI RGGWCCY 4 cut(s) 961, 1268, 1706, 2549
PspXI VCTCGAGB 1 cut(s) 2073
PsrI GAACNNNNNNTAC 2 cut(s) 1220, 1252
PstI CTGCAG 1 cut(s) 2254
PstNI CAGNNNCTG 1 cut(s) 2234
PsuI RGATCY 4 cut(s) 898, 1045, 2116, 3187
PvuII CAGCTG 3 cut(s) 916, 2111, 2180
RsaI GTAC 3 cut(s) 815, 1170, 2540
RsaNI GTAC 3 cut(s) 814, 1169, 2539
RseI CAYNNNNRTG 4 cut(s) 1994, 2046, 2517, 3136
SacI GAGCTC 1 cut(s) 1458
SatI GCNGC 7 cut(s) 89, 776, 2178, 2383, 2883, 3066, 3096
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 4 cut(s) 164, 253, 1258, 2240
SduI GDGCHC 9 cut(s) 476, 663, 1458, 1608, 1667, 2053, 2074, 2359, 2659
SfaNI GCATC 5 cut(s) 540, 1113, 2521, 2836, 3052
SfcI CTRYAG 1 cut(s) 2250
Sfr274I CTCGAG 1 cut(s) 2073
SfuI TTCGAA 1 cut(s) 278
SinI GGWCC 8 cut(s) 689, 961, 1070, 1268, 1706, 1853, 2549, 2639
SlaI CTCGAG 1 cut(s) 2073
SmiMI CAYNNNNRTG 4 cut(s) 1994, 2046, 2517, 3136
SmlI CTYRAG 4 cut(s) 265, 436, 523, 2073
SmoI CTYRAG 4 cut(s) 265, 436, 523, 2073
SphI GCATGC 1 cut(s) 1441
SsiI CCGC 6 cut(s) 254, 558, 1837, 2866, 2997, 3022
SspI AATATT 3 cut(s) 346, 1612, 2752
SspMI CTAG 3 cut(s) 653, 1043, 1421
SstI GAGCTC 1 cut(s) 1458
StyI CCWWGG 4 cut(s) 306, 2386, 2481, 3121
TaaI ACNGT 8 cut(s) 1293, 1365, 1417, 1733, 2434, 2732, 2807, 3138
TaiI ACGT 4 cut(s) 2050, 2277, 2692, 3148
TaqI TCGA 6 cut(s) 278, 320, 663, 1121, 2074, 2623
TatI WGTACW 2 cut(s) 813, 1168
TfiI GAWTC 8 cut(s) 280, 485, 820, 1022, 1693, 1877, 2240, 2839
TscAI CASTG 6 cut(s) 855, 868, 1486, 1593, 2254, 2904
TseFI GTSAC 6 cut(s) 846, 1084, 1282, 2186, 2223, 3073
TseI GCWGC 7 cut(s) 88, 775, 2177, 2382, 2882, 3065, 3095
Tsp45I GTSAC 6 cut(s) 846, 1084, 1282, 2186, 2223, 3073
TspGWI ACGGA 1 cut(s) 2146
TspRI CASTG 6 cut(s) 855, 868, 1486, 1593, 2254, 2904
Van91I CCANNNNNTGG 3 cut(s) 517, 1850, 2234
Vha464I CTTAAG 2 cut(s) 436, 523
VpaK11BI GGWCC 8 cut(s) 689, 961, 1070, 1268, 1706, 1853, 2549, 2639
XapI RAATTY 3 cut(s) 333, 1201, 2495
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 3 cut(s) 734, 1441, 1999
XcmI CCANNNNNNNNNTGG 1 cut(s) 2649
XhoI CTCGAG 1 cut(s) 2073
XmiI GTMKAC 1 cut(s) 3142
XmnI GAANNNNTTC 3 cut(s) 1697, 1901, 2838
XspI CTAG 3 cut(s) 653, 1043, 1421
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.