MD10G1252300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007159
Physical Location & Seq
Reverse (-)
34515498 .. 34520373
4876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1252300.v1.1.491

Sequence Viewer

Length: 3192 bp
ATGCTCCGTCGGCTTTTTGTGGTGTCCCTTCTGTTTATTTCTGCCATGGGGCAGCTCCCTTCGCAGGATATTTTGGCGCTGCTGGAGTTCAAGAAGGGTATCAAGCATGATCCCACTGGCTATGTCCTCAATTCTTGGAATGACGAGTCCATTGACTTTGATGGCTGCCCTTCTTCTTGGAATGGAGTCGTGTGTAATGGCGGAAATGTTGCTGGAGTTGTCCTTGATAATTTGAGTCTCTCTGCGGATGTGGACTTGAGCGTGTTTGCGAATCTAACGAAGCTTGTGAGACTTTCCATGGCCAACAATTCGATAATGGGGAAGTTTCCCGACAACATAGCTGACTTCAAAAGCCTTGAGTTTCTTGACCTGTCCAACAATTTATTTTCTTCACCTTTACCACCTGGGATTGGTAGGTTAGGGAGCTTAAGGAACCTCTCATTAGCTGGTAACAACTTCTCAGGCTCTATTCCAGATTCAATTTCAGGCCTTTCATCAGTCCAGTCATTGGACTTAAGTCGCAACTCCTTGTCCGGGCCACTGCCAGCAGCATTGACAAAGCTGCCTAAACTGGTGTCTCTAAATCTGTCATCGAACGGATTTACCGGGCAAATACCAAAGGGGTTCGAGCTCATTTCAAGTCTTGACGTGCTTGACTTGCATGGCAACATGCTCGATGGTAATATGGATCTGGAATTTTTGATGCTATCTGAGGTCACTCATGTTGATTTGAGTGACAATATGTTTGTTAGTTCCAGTTCACAACAGCAGAAATTTCTGCCTCGCCTTTCGGAGACTATAAAATACTTGAATCTTAGCCACAATCAGTTCAATGGGTCACTGGTAAGCGGCGGTGAGCTACAGATGTTTGAAAACTTGAAGGTGTTGGATCTCAGCTACAACCAGTTGTCAGGGGAATTGCCTGGATTTAATTTCGTGTATGACCTCCAAGTCCTCAAGCTGAGCAACAACAGATTTACTGGAGATATTCCTAATGGTCTTTTAAAAGGTGATTCTCTAGTTGTTAGTGAACTAGATTTGAGCGGCAACAATCTCTCAGGGCCAATAAATATGATTACGTCAACAAATTTGCGTTTCCTCAATCTCTCCTCAAATGGGCTAACTGGTGAGTTACCCTTGCTGACCGGAAGTTGTGCTGTGCTTGATCTATCGAATAACAAATTTAAAGGAAACTTAACCAGGATGGTGAAATGGGGGAACATCAAATATCTTGATCTGAGTCAAAATCGTTTGACAGGACCCATCCCTGATGTTACTCCACAGTTCTTGCGTTTAAATTATCTCAACCTCTCCCATAACACCCTTAGTAGTTCTATAGCAAGTGTTATAACACAATATCCAAGGATTAGTGTGCTTGATCTCAGTTCCAATCAACTCGATGGAACAATTCTAGCTGAATTGCTAACAATGCCCACTTTACAAGAACTTCACCTTCGTGATAATCTTCTCACCGGGTCTATTAATATCTCATCTCCTGTGTCAAGTGAGTCAAATCTTCAGGTTCTTGATCTTTCTCAGAACCAGCTTAGTGGCTATTTTCCTGATCATTTTGGTTCATTGAAGGGACTTAAAGTATTCAATATTGCTAGAAATAATTTTTCTGGCTCTCTTCCAACTTCAGTCACCAACATCAGCACATTAAGCTCATTCGACATATCACAGAATCATTTTACAGGTCACTTGCCTGACAACTTGCCTACTAGTCTTGAAAGCTTTAATGCTTCATACAATGATCTTTCTGGAGATGTCCCGGAAAATCTGGGAAAGTTTCCAAGATCTTCCTTTTTCCCAGGGAATGCACGTTTACAATTTCCAAATGGTCCTCCGGGATCCAATGGTACCGAAAATGAAAGTTCAAAGAGGGGACCAATCAGCACAATGGTCAAAGTAATCATTATCGTTTCATGTGTGGTTGCCGTCTTCATTCTTCTTCTGCTTGCTATCTTCGTACACTACATCCGCATGTCAAGAAGAATCCCGTCAGAGCATACTACAAGCAAAGACATCCACAGGCGCGCTCAACCAACTCAATCTGGTGTTCGTGGAACAGCCACCGGTGGCTCTTTGGTTGTTTCAGATGAGGATCTCATGGCTTCACAAAAAGGATCATCATCTGAAATTGTCAGCCCCAACAAAAAAGTTGCGGCTGTAGCTGGTTTCTCCCCTTCAAAGCACAGCCATTTCTCTTGGTCACCAGAGTCTGGAGAGTCAGTCACTACAGAAAACCTTGCAAGACTGGATGTGAGGTCGCCGGATAAATTGGTCGGTGAGTTGCATTTTCTTGATGACACAATCGCATTGACCCCCGAGGAGCTGTCAAGGGCTCCTGCTGAAGTTCTGGGAAGAAGTAGCCATGGGACTTCTTACAGGGCAACACTTGATAACGGGTTGCTCTTGACAGTTAAGTGGTTGAGAGAAGGGGTGGCGAAACAGAAAAAGGAGTTTGCTAAGGAGGCTAAAAAGTTTGCGAACATGAGGCATCCGAATGTTGTAGGTTTGAAAGGGTACTATTGGGGCCCCACACAGCATGAGAAGCTCATTCTTTCCGATTATATCTCACCTGGAAGTCTTGCAAGCTTTCTTTATGATCGACCAGGAAGAAAAGGTCCACCACTGATCTGGGCCCAAAGGCTCAAAATTGCAGTTGACGTTGCACGCGGTCTGAACTATCTCCATTTCGACCGTGCTGTTCCACACGGTAACCTCAAAGCATCAAATATTTTGTTGGACGGGCCAGACCTGAATGCCCGTGTTGCTGATTACTGCCTCCACCGCCTCATGACACAAACTGGCACCATTGAACAGATTCTCGATGCTGGTGTCTTGGGTTACCGTGCACCAGAGTTGGCTGCTTCCAAGAAGCCGCTTCCCTCCTTTAAGTCAGACATTTACGCCTTTGGAGTGGTAATGCTGGAACTCTTAACTGGAAGGTGTGCGGGCGATGTGATTTCAGGTGAAGGAAGTGGTGTTGATTTGACAGACTGGGTCCGGCTAAGGGTATCAGAAGGGCGCGGCTCTGAATGTTTTGACGCTGCATTGGTGCCGGAAATGGGAATGCCAGCAGCAGAGAAGGGAATGAAGGAGGTCCTTGGAATATCGTTACGATGTTTAAGAAGTGTTGGGGAGAGGCCAGGTATTAAGAACATCTATGAGGATCTCTCCTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1064

Amino Acids

115.39

Weight (kDa)

6.19

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 66 1.3e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 137 - 277 6.5e-08 Leucine-rich repeat region
LRR_8 PF13855 142 - 200 5.2e-10 Leucine rich repeat
LRR_8 PF13855 407 - 466 2.6e-06 Leucine rich repeat
LRR_8 PF13855 504 - 564 1.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 785 - 983 1.9e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 785 - 984 1.3e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1349
AasI GACNNNNNNGTC 1 cut(s) 950
Acc65I GGTACC 1 cut(s) 1859
AccB1I GGYRCC 3 cut(s) 1859, 2813, 3061
AccB7I CCANNNNNTGG 2 cut(s) 508, 2222
AccBSI CCGCTC 1 cut(s) 1044
AccII CGCG 3 cut(s) 2037, 2679, 3033
AclWI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2112, 2134, 3183
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 4 cut(s) 695, 773, 1087, 1181
AcuI CTGAAG 3 cut(s) 1502, 1623, 2373
AfaI GTAC 3 cut(s) 1861, 1971, 2528
AfiI CCNNNNNNNGG 8 cut(s) 64, 410, 508, 534, 1116, 2222, 3016, 3071
AflII CTTAAG 2 cut(s) 427, 514
AgeI ACCGGT 1 cut(s) 2075
AhlI ACTAGT 1 cut(s) 1721
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 7 cut(s) 403, 922, 1199, 1810, 2581, 2614, 3151
AleI CACNNNNGTG 1 cut(s) 1455
Alw21I GWGCWC 2 cut(s) 633, 2860
Alw26I GTCTC 4 cut(s) 242, 283, 582, 788
Alw44I GTGCAC 1 cut(s) 2856
AlwI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2112, 2134, 3183
AlwNI CAGNNNCTG 1 cut(s) 2222
Ama87I CYCGRG 1 cut(s) 2327
AoxI GGCC 8 cut(s) 300, 487, 536, 1061, 2536, 2643, 2753, 3149
ApaI GGGCCC 2 cut(s) 2540, 2647
ApaLI GTGCAC 1 cut(s) 2856
ApeKI GCWGC 8 cut(s) 52, 79, 165, 548, 562, 2870, 3053, 3083
ApoI RAATTY 4 cut(s) 695, 773, 1087, 1181
ArsI GACNNNNNNTTYG 2 cut(s) 728, 760
AseI ATTAAT 1 cut(s) 1482
AsiGI ACCGGT 1 cut(s) 2075
Asp700I GAANNNNTTC 1 cut(s) 2826
Asp718I GGTACC 1 cut(s) 1859
AspLEI GCGC 4 cut(s) 79, 2037, 2039, 3033
AsuC2I CCSGG 5 cut(s) 535, 607, 1474, 1772, 1848
AvaI CYCGRG 1 cut(s) 2327
AvaII GGWCC 6 cut(s) 1259, 1841, 1886, 2627, 3007, 3106
BaeGI GKGCMC 3 cut(s) 2540, 2647, 2860
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 1850
BanI GGYRCC 3 cut(s) 1859, 2813, 3061
BanII GRGCYC 4 cut(s) 633, 2347, 2540, 2647
BbsI GAAGAC 1 cut(s) 1933
Bbv12I GWGCWC 2 cut(s) 633, 2860
BbvI GCAGC 8 cut(s) 64, 66, 152, 549, 560, 2857, 3040, 3095
BccI CCATC 5 cut(s) 155, 671, 1198, 1271, 1394
BceAI ACGGC 1 cut(s) 1922
BcgI CGANNNNNNTGC 2 cut(s) 655, 689
BciT130I CCWGG 7 cut(s) 405, 924, 1201, 1812, 2583, 2616, 3153
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 5 cut(s) 535, 607, 1474, 1772, 1848
BcoDI GTCTC 4 cut(s) 242, 283, 582, 788
BcuI ACTAGT 1 cut(s) 1721
BfaI CTAG 5 cut(s) 1019, 1034, 1412, 1608, 1722
BfmI CTRYAG 4 cut(s) 860, 1335, 2169, 2238
BfoI RGCGCY 1 cut(s) 80
BfrI CTTAAG 2 cut(s) 427, 514
BglII AGATCT 1 cut(s) 1796
BlpI GCTNAGC 1 cut(s) 962
Bme18I GGWCC 6 cut(s) 1259, 1841, 1886, 2627, 3007, 3106
BmeT110I CYCGRG 1 cut(s) 2327
BmgBI CACGTC 1 cut(s) 649
BmrI ACTGGG 1 cut(s) 3013
BmsI GCATC 4 cut(s) 693, 2509, 2741, 2824
BmuI ACTGGG 1 cut(s) 3013
BoxI GACNNNNGTC 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 1933
BplI GAGNNNNNCTC 1 cut(s) 3164
BpmI CTGGAG 5 cut(s) 104, 234, 1002, 1782, 2244
Bpu10I CCTNAGC 2 cut(s) 2469, 3014
Bpu1102I GCTNAGC 1 cut(s) 962
BpuEI CTTGAG 3 cut(s) 277, 377, 941
BpuMI CCSGG 5 cut(s) 535, 607, 1474, 1772, 1848
BsaBI GATNNNNATC 2 cut(s) 2103, 2131
BsaJI CCNNGG 9 cut(s) 45, 297, 404, 1361, 1810, 1811, 2328, 2374, 3109
BsaWI WCCGGW 2 cut(s) 1145, 2075
BsaXI ACNNNNNCTCC 2 cut(s) 2217, 2247
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 410, 508, 534, 1116, 2222, 3016, 3071
Bse118I RCCGGY 1 cut(s) 2075
Bse8I GATNNNNATC 2 cut(s) 2103, 2131
BseBI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2583, 2616, 3153
BseDI CCNNGG 9 cut(s) 45, 297, 404, 1361, 1810, 1811, 2328, 2374, 3109
BseGI GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2266, 2500
BseJI GATNNNNATC 2 cut(s) 2103, 2131
BseLI CCNNNNNNNGG 8 cut(s) 64, 410, 508, 534, 1116, 2222, 3016, 3071
BseMII CTCAG 8 cut(s) 474, 702, 907, 953, 1071, 1229, 1396, 1550
BsePI GCGCGC 1 cut(s) 2035
BseRI GAGGAG 3 cut(s) 1099, 2345, 3172
BseSI GKGCMC 3 cut(s) 2540, 2647, 2860
BseXI GCAGC 8 cut(s) 64, 66, 152, 549, 560, 2857, 3040, 3095
Bsh1236I CGCG 3 cut(s) 2037, 2679, 3033
Bsh1285I CGRYCG 1 cut(s) 2704
BshFI GGCC 8 cut(s) 302, 489, 538, 1063, 2538, 2645, 2755, 3151
BshNI GGYRCC 3 cut(s) 1859, 2813, 3061
BshTI ACCGGT 1 cut(s) 2075
BsiEI CGRYCG 1 cut(s) 2704
BsiHKAI GWGCWC 2 cut(s) 633, 2860
BsiHKCI CYCGRG 1 cut(s) 2327
BslFI GGGAC 5 cut(s) 10, 1599, 1754, 1899, 2392
BslI CCNNNNNNNGG 8 cut(s) 64, 410, 508, 534, 1116, 2222, 3016, 3071
BsmAI GTCTC 4 cut(s) 242, 283, 582, 788
BsmFI GGGAC 5 cut(s) 10, 1599, 1754, 1899, 2392
BsmI GAATGC 3 cut(s) 1822, 2770, 3081
BsnI GGCC 8 cut(s) 302, 489, 538, 1063, 2538, 2645, 2755, 3151
BsoBI CYCGRG 1 cut(s) 2327
Bsp120I GGGCCC 2 cut(s) 2536, 2643
Bsp1286I GDGCHC 5 cut(s) 633, 2347, 2540, 2647, 2860
Bsp1720I GCTNAGC 1 cut(s) 962
Bsp19I CCATGG 3 cut(s) 45, 297, 2374
BspANI GGCC 8 cut(s) 302, 489, 538, 1063, 2538, 2645, 2755, 3151
BspCNI CTCAG 8 cut(s) 473, 703, 906, 954, 1070, 1230, 1395, 1549
BspFNI CGCG 3 cut(s) 2037, 2679, 3033
BspHI TCATGA 1 cut(s) 2799
BspPI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2112, 2134, 3183
BspT107I GGYRCC 3 cut(s) 1859, 2813, 3061
BspTI CTTAAG 2 cut(s) 427, 514
BsrBI CCGCTC 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 2075
BssAI RCCGGY 1 cut(s) 2075
BssECI CCNNGG 9 cut(s) 45, 297, 404, 1361, 1810, 1811, 2328, 2374, 3109
BssHII GCGCGC 1 cut(s) 2035
BssT1I CCWWGG 5 cut(s) 45, 297, 1361, 2374, 3109
Bst2UI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2583, 2616, 3153
Bst4CI ACNGT 5 cut(s) 1284, 2422, 2705, 2720, 2855
Bst6I CTCTTC 1 cut(s) 1635
BstAFI CTTAAG 2 cut(s) 427, 514
BstC8I GCNNGC 7 cut(s) 546, 1959, 2037, 2596, 2677, 2959, 3081
BstDSI CCRYGG 3 cut(s) 45, 297, 2374
BstEII GGTNACC 3 cut(s) 2211, 2720, 2849
BstF5I GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2266, 2500
BstFNI CGCG 3 cut(s) 2037, 2679, 3033
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 4 cut(s) 79, 2037, 2039, 3033
BstMAI GTCTC 4 cut(s) 242, 283, 582, 788
BstMCI CGRYCG 1 cut(s) 2704
BstNI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2583, 2616, 3153
BstNSI RCATGY 2 cut(s) 673, 1987
BstPAI GACNNNNGTC 1 cut(s) 516
BstPI GGTNACC 3 cut(s) 2211, 2720, 2849
BstSFI CTRYAG 4 cut(s) 860, 1335, 2169, 2238
BstSLI GKGCMC 3 cut(s) 2540, 2647, 2860
BstUI CGCG 3 cut(s) 2037, 2679, 3033
BstV1I GCAGC 8 cut(s) 64, 66, 152, 549, 560, 2857, 3040, 3095
BstV2I GAAGAC 1 cut(s) 1933
BstX2I RGATCY 6 cut(s) 688, 889, 1796, 1850, 2104, 3175
BstXI CCANNNNNNTGG 2 cut(s) 1550, 2640
BstYI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2104, 3175
BsuRI GGCC 8 cut(s) 302, 489, 538, 1063, 2538, 2645, 2755, 3151
BtgI CCRYGG 3 cut(s) 45, 297, 2374
BtgZI GCGATG 1 cut(s) 2976
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2266, 2500
BtsI GCAGTG 1 cut(s) 539
BtsIMutI CAGTG 4 cut(s) 114, 539, 839, 2633
Cac8I GCNNGC 7 cut(s) 546, 1959, 2037, 2596, 2677, 2959, 3081
CaiI CAGNNNCTG 1 cut(s) 2222
CciI TCATGA 1 cut(s) 2799
CfoI GCGC 4 cut(s) 79, 2037, 2039, 3033
Cfr10I RCCGGY 1 cut(s) 2075
CseI GACGC 1 cut(s) 3059
Csp6I GTAC 3 cut(s) 1860, 1970, 2527
CspAI ACCGGT 1 cut(s) 2075
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviQI GTAC 3 cut(s) 1860, 1970, 2527
DraI TTTAAA 3 cut(s) 1005, 1186, 1296
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 1635
EarI CTCTTC 1 cut(s) 1635
EciI GGCGGA 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 5 cut(s) 45, 297, 1361, 2374, 3109
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 4 cut(s) 633, 2347, 2540, 2647
Eco47I GGWCC 6 cut(s) 1259, 1841, 1886, 2627, 3007, 3106
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 3 cut(s) 1502, 1623, 2373
Eco88I CYCGRG 1 cut(s) 2327
Eco91I GGTNACC 3 cut(s) 2211, 2720, 2849
EcoICRI GAGCTC 1 cut(s) 631
EcoO109I RGGNCCY 4 cut(s) 1259, 2536, 2537, 3106
EcoO65I GGTNACC 3 cut(s) 2211, 2720, 2849
EcoRII CCWGG 7 cut(s) 403, 922, 1199, 1810, 2581, 2614, 3151
EcoT14I CCWWGG 5 cut(s) 45, 297, 1361, 2374, 3109
EcoT38I GRGCYC 4 cut(s) 633, 2347, 2540, 2647
ErhI CCWWGG 5 cut(s) 45, 297, 1361, 2374, 3109
FaqI GGGAC 5 cut(s) 10, 1599, 1754, 1899, 2392
FauI CCCGC 1 cut(s) 2950
FbaI TGATCA 1 cut(s) 1564
FokI GGATG 7 cut(s) 260, 1216, 1250, 1964, 2012, 2273, 2487
FriOI GRGCYC 4 cut(s) 633, 2347, 2540, 2647
FspBI CTAG 5 cut(s) 1019, 1034, 1412, 1608, 1722
GlaI GCGC 4 cut(s) 78, 2036, 2038, 3032
GsuI CTGGAG 5 cut(s) 104, 234, 1002, 1782, 2244
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 8 cut(s) 302, 489, 538, 1063, 2538, 2645, 2755, 3151
HgaI GACGC 1 cut(s) 3059
HhaI GCGC 4 cut(s) 79, 2037, 2039, 3033
Hin6I GCGC 4 cut(s) 77, 2035, 2037, 3031
HinP1I GCGC 4 cut(s) 77, 2035, 2037, 3031
HincII GTYRAC 2 cut(s) 1083, 2668
HindII GTYRAC 2 cut(s) 1083, 2668
HindIII AAGCTT 3 cut(s) 281, 1732, 2596
Hpy166II GTNNAC 9 cut(s) 253, 761, 1031, 1083, 1826, 1972, 2630, 2668, 2858
Hpy8I GTNNAC 9 cut(s) 253, 761, 1031, 1083, 1826, 1972, 2630, 2668, 2858
Hpy99I CGWCG 1 cut(s) 12
HpyCH4III ACNGT 5 cut(s) 1284, 2422, 2705, 2720, 2855
HpyCH4IV ACGT 4 cut(s) 648, 1079, 1822, 2670
HpyCH4V TGCA 9 cut(s) 661, 1820, 2252, 2296, 2594, 2663, 2675, 2858, 3056
HpySE526I ACGT 4 cut(s) 648, 1079, 1822, 2670
HspAI GCGC 4 cut(s) 77, 2035, 2037, 3031
KpnI GGTACC 1 cut(s) 1863
Ksp22I TGATCA 1 cut(s) 1564
LmnI GCTCC 5 cut(s) 9, 60, 423, 2332, 2350
Lsp1109I GCAGC 8 cut(s) 64, 66, 152, 549, 560, 2857, 3040, 3095
LweI GCATC 4 cut(s) 693, 2509, 2741, 2824
MaeI CTAG 5 cut(s) 1019, 1034, 1412, 1608, 1722
MaeII ACGT 4 cut(s) 648, 1079, 1822, 2670
MbiI CCGCTC 1 cut(s) 1044
MflI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2104, 3175
MhlI GDGCHC 5 cut(s) 633, 2347, 2540, 2647, 2860
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 7 cut(s) 155, 195, 244, 1249, 1517, 2228, 2237
MmeI TCCRAC 4 cut(s) 399, 867, 1658, 2727
Mox20I TGGCCA 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 2826
MscI TGGCCA 1 cut(s) 302
MslI CAYNNNNRTG 3 cut(s) 1455, 1982, 2505
Msp20I TGGCCA 1 cut(s) 302
MspCI CTTAAG 2 cut(s) 427, 514
Mva1269I GAATGC 3 cut(s) 1822, 2770, 3081
MvaI CCWGG 7 cut(s) 405, 924, 1201, 1812, 2583, 2616, 3153
MvnI CGCG 3 cut(s) 2037, 2679, 3033
NciI CCSGG 5 cut(s) 535, 607, 1474, 1772, 1848
NcoI CCATGG 3 cut(s) 45, 297, 2374
NmuCI GTSAC 7 cut(s) 715, 734, 837, 1642, 1697, 2211, 2233
NspI RCATGY 2 cut(s) 673, 1987
OliI CACNNNNGTG 1 cut(s) 1455
PagI TCATGA 1 cut(s) 2799
PasI CCCWGGG 1 cut(s) 1811
PauI GCGCGC 1 cut(s) 2035
PceI AGGCCT 1 cut(s) 489
PctI GAATGC 3 cut(s) 1822, 2770, 3081
PdmI GAANNNNTTC 1 cut(s) 2826
PfeI GAWTC 7 cut(s) 271, 476, 811, 1013, 1684, 1995, 2827
PflFI GACNNNGTC 1 cut(s) 3005
PflMI CCANNNNNTGG 2 cut(s) 508, 2222
PfoI TCCNGGA 2 cut(s) 1770, 1846
PinAI ACCGGT 1 cut(s) 2075
PleI GAGTC 7 cut(s) 154, 194, 243, 1248, 1516, 2227, 2236
PpsI GAGTC 7 cut(s) 154, 194, 243, 1248, 1516, 2227, 2236
PpuMI RGGWCCY 2 cut(s) 1259, 3106
PshAI GACNNNNGTC 1 cut(s) 516
PshBI ATTAAT 1 cut(s) 1482
PsiI TTATAA 1 cut(s) 1349
Psp124BI GAGCTC 1 cut(s) 633
Psp5II RGGWCCY 2 cut(s) 1259, 3106
Psp6I CCWGG 7 cut(s) 403, 922, 1199, 1810, 2581, 2614, 3151
PspEI GGTNACC 3 cut(s) 2211, 2720, 2849
PspGI CCWGG 7 cut(s) 403, 922, 1199, 1810, 2581, 2614, 3151
PspOMI GGGCCC 2 cut(s) 2536, 2643
PspPPI RGGWCCY 2 cut(s) 1259, 3106
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2104, 3175
PsyI GACNNNGTC 1 cut(s) 3005
PteI GCGCGC 1 cut(s) 2035
RsaI GTAC 3 cut(s) 1861, 1971, 2528
RsaNI GTAC 3 cut(s) 1860, 1970, 2527
RseI CAYNNNNRTG 3 cut(s) 1455, 1982, 2505
SacI GAGCTC 1 cut(s) 633
SchI GAGTC 7 cut(s) 155, 195, 244, 1249, 1517, 2228, 2237
SduI GDGCHC 5 cut(s) 633, 2347, 2540, 2647, 2860
SfaNI GCATC 4 cut(s) 693, 2509, 2741, 2824
SfcI CTRYAG 4 cut(s) 860, 1335, 2169, 2238
SgrAI CRCCGGYG 1 cut(s) 2075
SinI GGWCC 6 cut(s) 1259, 1841, 1886, 2627, 3007, 3106
SmiMI CAYNNNNRTG 3 cut(s) 1455, 1982, 2505
SmlI CTYRAG 5 cut(s) 256, 356, 427, 514, 956
SmoI CTYRAG 5 cut(s) 256, 356, 427, 514, 956
SpeI ACTAGT 1 cut(s) 1721
SseBI AGGCCT 1 cut(s) 489
SspI AATATT 2 cut(s) 1603, 2740
SspMI CTAG 5 cut(s) 1019, 1034, 1412, 1608, 1722
SstI GAGCTC 1 cut(s) 633
StuI AGGCCT 1 cut(s) 489
StyI CCWWGG 5 cut(s) 45, 297, 1361, 2374, 3109
TaaI ACNGT 5 cut(s) 1284, 2422, 2705, 2720, 2855
TaiI ACGT 4 cut(s) 651, 1082, 1825, 2673
TauI GCSGC 5 cut(s) 852, 1047, 2168, 2887, 3036
TfiI GAWTC 7 cut(s) 271, 476, 811, 1013, 1684, 1995, 2827
TscAI CASTG 4 cut(s) 121, 546, 846, 2640
TseFI GTSAC 7 cut(s) 715, 734, 837, 1642, 1697, 2211, 2233
TseI GCWGC 8 cut(s) 52, 79, 165, 548, 562, 2870, 3053, 3083
Tsp45I GTSAC 7 cut(s) 715, 734, 837, 1642, 1697, 2211, 2233
TspDTI ATGAA 7 cut(s) 483, 1566, 1734, 1884, 1914, 1933, 3113
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 4 cut(s) 121, 546, 846, 2640
Tth111I GACNNNGTC 1 cut(s) 3005
Van91I CCANNNNNTGG 2 cut(s) 508, 2222
Vha464I CTTAAG 2 cut(s) 427, 514
VneI GTGCAC 1 cut(s) 2856
VpaK11BI GGWCC 6 cut(s) 1259, 1841, 1886, 2627, 3007, 3106
VspI ATTAAT 1 cut(s) 1482
XapI RAATTY 4 cut(s) 695, 773, 1087, 1181
XceI RCATGY 2 cut(s) 673, 1987
XcmI CCANNNNNNNNNTGG 1 cut(s) 2637
XmnI GAANNNNTTC 1 cut(s) 2826
XspI CTAG 5 cut(s) 1019, 1034, 1412, 1608, 1722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.