pycom10g20810

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007159
Physical Location & Seq
Reverse (-)
23595998 .. 23599458
3461 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20810.1

Sequence Viewer

Length: 3168 bp
ATGCTCCGTTGGCTTTTTGTGGTGTCCCTTCTGTTTATTTCTGCCATGGGGCAGCTCCCTTCGCAGGATATTTTGGCGCTGCTGGAGTTCAAGAAGGGTATCAAGCATGATCCCACTGGCTATGTTCTCAATTCTTGGAATGACGAGTCCATTGACTTTGATGGCTGCCCTTCTTCTTGGAATGGAGTCGTGTGTAATGGCGGAAATGTTGCCGGAGTTGTCCTTGATAATTTGAGTCTCTCCGCGGATGTGGACTTGAGCGTGTTTGCGAATCTGACGAAGCTTGTGAGACTTTCCATGGCCAACAATTCGATAATGGGGAAGTTTCCCGGCAACATAGCTGACTTCAAAAGCCTTGAGTTTCTTGACCTGTCCAACAATTTATTTTCTTCACCTTTACCACCTGGGATTGGTAGGTTAGGGAGCTTAAGGAACCTCTCATTAGCTGGTAACAACTTCTCAGGCTCTATTCCAGATTCAATTTCAGGCCTTTCCTCAGTTCAGTCATTGGACTTAAGTCGCAACTCCTTGTCCGGGCCACTGCCAGCAGCATTGACAAAGCTGCCTAAACTGGTGTCTCTAAATCTGTCATTGAACGGATTTACCAGGCAAATACCAAAGGGGTTTGAGCTAATTTCAAGTCTTGACGTGCTTGACTTGCATGGCAACATGCTCGATGGTAATATGGATCTGGAATTTTTGATGCTATCTGAGGTCACTCATGTTGATTTGAGTGGCAATATGTTTGTTAGTTCCAGTTCACAACAGCAGAAATTTCTGCCTCGCCTTTCGGAGACTATAAAGTACTTGAATCTTAGCCACAATCAGCTCAATGGGTCCCTGGTAAGCGGCGGTGAGCTACAGATGTTTGAAAACTTGAAGGTGTTGGATCTCAGCTATAACCAGTTGTCAGGGGAATTGCCTGGATTTAATTTCGTGTATGACCTCGAAGTCCTCAAGCTGAGCAACAACAGATTTACTGGAGATATTCCTAATGGTCTTTTAAAAGGTGATTCTCTGGTTGTTAGTGAACTAGATTTGAGCGGCAACAATCTCTCAGGGCCAATAAATATGATTACGTCAACAAATTTGCGTTTCCTCAATCTCTCCTCAAATGGGCTAACTGGTGAGTTACCCTTGCTGACCGGAAGTTGTGCTGTGCTTGATCTATCGAATAACAAATTTGAAGGAAACTTAACCAGGATGGTGAAATGGGGGAACATCGAATATCTTGATCTGAGTCAAAATCGTTTGACAGGACCCATCCCTGATGTTACCCCACAGTTCTTGCGTTTAAATTATCTCAACCTCTCCCATAACACCCTTCGTAGTTCTATAGCAAGTGTTATAACACAGTATCCAAAGATTAGTGTGCTTGATCTCAGTTCCAATCATCTCGATGGAACAATTCTAGCCGAATTGCTAGCAATGCCCACTTTACAAGAACTTCACCTTCGTGATAATCTTCTCACCGGGTCTATTAATATCTCATCTCCTGTGTCAAGTGAGTCTAATCTTCAGGTTCTTGATCTTTCTCAGAACCAGCTTAGTGGCTATTTTCCTGATCATTTTGGTTCATTGAAGGGACTTAAAGTATTCAATGTTGCTAGAAATAATTTTTCTGGCTCCCTTCCAACTTCAGTCACCAACATCAGCACATTAAGTTCATTCGACATATCACAGAATCATTTTACAGGTCCTTTGCCGAACAACTTGCCTACTAGTCTTGAAAGTTTTAATGCTTCATACAATGATCTTTCTGGAGATGTCCCGGAAAATCTGAGAAAGTTTCCAAGATCTTCCTTCTTCCCAGGGAATGCACGTTTACAATTTCCAAATGGTCCTCCGGGATCCAATGGTACTGAAAATGAAAGTTCAAAGAGGAGACCAATCAGCACAATGGTCAAAGTAATCATTATAGTTTCATGTGTGGTTGCCGTCTTCATTCTTCTTCTGCTTGCTATCTTCGTACACTACATCCGCATGTCAAGAAGAATCCCGTCAGAGCATACCACAAGCAAAGACATCCACAGGCACGCTCAACCAACTCAATCTGGTGTTCGTGGAACTTCAGCTGAGGATCTCATGGCTTCACAAAAAGGATCATCATCTGAAATTGTCAGCCCCGACAAAAAAGTTGCAGCTGTAGCTGGTTTTTCTCCTTCAAAGCACAGCCATTTCTCTTGGTCACCAGAGTCTGGAGAGTCGGTCACTACAGAAAACCTTGCAAGACTGGATGTGAGGTCGCCGGATAAATTGGTCGGTGAGTTGCATTTTCTTGATGACACAATCGCATTGACCCCCGAGGAGCTGTCAAGGGCTCCTGCTGAAGTTCTGGGAAGAAGTAGCCATGGGACTTCTTACAGGGCAACACTTGATAACGGGTTGCTCTTGACAGTGAAGTGGTTGAGAGAAGGGGTGGCGAAACAGAAAAAGGAGTTTGCTAAGGAGGCTAAAAAGTTTGCGAACATGAGGCATCCAAATGTTGTAGGTTTGAAGGGGTACTATTGGGGCCCCACACAGCATGAGAAGCTCATTCTTTCCGATTATATCTCACCTGGAAGTCTTGCAAGCTTTCTTTATGATCGACCAGGAAGAAAGGGTCAACCACTGACCTGGGCCCAAAGGCTCAAAATTGCAGTTGACGTTGCACGTGGTCTGAACTATCTCCATTTTGACCGTGCTGTTCCACACGGTAACCTCAAAGCATCAAATATTTTGTTGGACGGGCCAGACCTGAATGCCCGTGTTGCTGATTACTGCCTCCACCGCCTCATGACACAAGCTGGCACCATTGAACAGATTCTCGATGCTGGTGTCTTGGGTTACCGTGCACCAGAGTTGGCTGCTTCCAAGAAGCCGCTTCCCTCCTTCAAGTCAGACATTTACGCCTTTGGAGTGGTAATGCTGGAACTCTTAACTGGAAGGTGTGCGGGCGATGTGATTTCAGGTGAAGGAAGTGGGGTTGATTTGACAGACTGGGTCCGGCTGAGGGTATCAGAAGGGCGTGGCTCTGAATGTTTTGACGCTGCATTGGTGCCGGAAATGGGAATGCCAGCAGCAGAGAAGGGAATGAAGGAGGTCCTTGGAATATCGTTACGATGTTTAAGAAGTGTTGGGGAGAGACCAGGTATTAAGAACATCTATGAGGATCTCTCCTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1056

Amino Acids

114.8

Weight (kDa)

6.22

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 21 - 66 1.3e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 137 - 223 2.8e-07 Leucine-rich repeat region
LRR_8 PF13855 142 - 199 1.3e-08 Leucine rich repeat
LRR_8 PF13855 407 - 466 2.3e-06 Leucine rich repeat
LRR_8 PF13855 504 - 564 4.8e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 777 - 975 1.5e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 777 - 976 2.1e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1349
AasI GACNNNNNNGTC 1 cut(s) 950
AccB1I GGYRCC 2 cut(s) 2789, 3037
AccB7I CCANNNNNTGG 1 cut(s) 2198
AccBSI CCGCTC 1 cut(s) 1044
AccII CGCG 1 cut(s) 245
AclWI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2088, 2110, 3159
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 4 cut(s) 695, 773, 1087, 1181
AcuI CTGAAG 4 cut(s) 1502, 1623, 2055, 2349
AcvI CACGTG 1 cut(s) 2654
AfaI GTAC 4 cut(s) 806, 1861, 1971, 2504
AfiI CCNNNNNNNGG 7 cut(s) 64, 410, 534, 1116, 2198, 2992, 3047
AflII CTTAAG 2 cut(s) 427, 514
AhlI ACTAGT 1 cut(s) 1721
AjiI CACGTC 1 cut(s) 649
AleI CACNNNNGTG 1 cut(s) 1455
Alw21I GWGCWC 1 cut(s) 2836
Alw26I GTCTC 6 cut(s) 242, 283, 582, 788, 1879, 3118
Alw44I GTGCAC 1 cut(s) 2832
AlwI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2088, 2110, 3159
AlwNI CAGNNNCTG 1 cut(s) 2198
Ama87I CYCGRG 1 cut(s) 2303
AoxI GGCC 7 cut(s) 300, 487, 536, 1061, 2512, 2619, 2729
ApaI GGGCCC 2 cut(s) 2516, 2623
ApaLI GTGCAC 1 cut(s) 2832
ApeKI GCWGC 9 cut(s) 52, 79, 165, 548, 562, 2141, 2846, 3029, 3059
ApoI RAATTY 4 cut(s) 695, 773, 1087, 1181
AseI ATTAAT 1 cut(s) 1482
Asp700I GAANNNNTTC 1 cut(s) 2802
AspLEI GCGC 1 cut(s) 79
AsuC2I CCSGG 5 cut(s) 330, 535, 1474, 1772, 1848
AsuNHI GCTAGC 1 cut(s) 1423
AvaI CYCGRG 1 cut(s) 2303
AvaII GGWCC 6 cut(s) 837, 1259, 1697, 1841, 2983, 3082
BaeGI GKGCMC 3 cut(s) 2516, 2623, 2836
BalI TGGCCA 1 cut(s) 302
BamHI GGATCC 1 cut(s) 1850
BanI GGYRCC 2 cut(s) 2789, 3037
BanII GRGCYC 3 cut(s) 2323, 2516, 2623
BbrPI CACGTG 1 cut(s) 2654
BbsI GAAGAC 1 cut(s) 1933
Bbv12I GWGCWC 1 cut(s) 2836
BbvCI CCTCAGC 2 cut(s) 2076, 2990
BbvI GCAGC 9 cut(s) 64, 66, 152, 549, 560, 2153, 2833, 3016, 3071
BccI CCATC 5 cut(s) 155, 671, 1198, 1271, 1394
BceAI ACGGC 1 cut(s) 1922
BcgI CGANNNNNNTGC 4 cut(s) 655, 689, 1696, 1730
BciVI GTATCC 1 cut(s) 1368
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 5 cut(s) 330, 535, 1474, 1772, 1848
BcoDI GTCTC 6 cut(s) 242, 283, 582, 788, 1879, 3118
BcuI ACTAGT 1 cut(s) 1721
BfaI CTAG 5 cut(s) 1034, 1412, 1424, 1608, 1722
BfmI CTRYAG 4 cut(s) 860, 1335, 2145, 2214
BfoI RGCGCY 1 cut(s) 80
BfrI CTTAAG 2 cut(s) 427, 514
BfuI GTATCC 1 cut(s) 1368
BglII AGATCT 1 cut(s) 1796
BlpI GCTNAGC 1 cut(s) 962
BmcAI AGTACT 1 cut(s) 806
Bme18I GGWCC 6 cut(s) 837, 1259, 1697, 1841, 2983, 3082
BmeT110I CYCGRG 1 cut(s) 2303
BmgBI CACGTC 1 cut(s) 649
BmrI ACTGGG 1 cut(s) 2989
BmsI GCATC 4 cut(s) 693, 2485, 2717, 2800
BmtI GCTAGC 1 cut(s) 1427
BmuI ACTGGG 1 cut(s) 2989
BoxI GACNNNNGTC 1 cut(s) 516
BpiI GAAGAC 1 cut(s) 1933
BplI GAGNNNNNCTC 1 cut(s) 3140
BpmI CTGGAG 4 cut(s) 104, 1002, 1782, 2220
Bpu10I CCTNAGC 3 cut(s) 2076, 2445, 2990
Bpu1102I GCTNAGC 1 cut(s) 962
BpuEI CTTGAG 3 cut(s) 277, 377, 941
BpuMI CCSGG 5 cut(s) 330, 535, 1474, 1772, 1848
BsaAI YACGTR 1 cut(s) 2654
BsaBI GATNNNNATC 1 cut(s) 2107
BsaI GGTCTC 2 cut(s) 1879, 3118
BsaWI WCCGGW 1 cut(s) 1145
BsaXI ACNNNNNCTCC 2 cut(s) 2193, 2223
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 410, 534, 1116, 2198, 2992, 3047
Bse1I ACTGG 9 cut(s) 121, 576, 756, 904, 985, 1129, 2238, 2926, 2984
Bse3DI GCAATG 1 cut(s) 1434
Bse8I GATNNNNATC 1 cut(s) 2107
BseGI GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2242, 2476
BseJI GATNNNNATC 1 cut(s) 2107
BseLI CCNNNNNNNGG 7 cut(s) 64, 410, 534, 1116, 2198, 2992, 3047
BseMI GCAATG 1 cut(s) 1434
BseNI ACTGG 9 cut(s) 121, 576, 756, 904, 985, 1129, 2238, 2926, 2984
BseRI GAGGAG 4 cut(s) 1099, 1897, 2321, 3148
BseSI GKGCMC 3 cut(s) 2516, 2623, 2836
BseXI GCAGC 9 cut(s) 64, 66, 152, 549, 560, 2153, 2833, 3016, 3071
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 7 cut(s) 302, 489, 538, 1063, 2514, 2621, 2731
BshNI GGYRCC 2 cut(s) 2789, 3037
BsiHKAI GWGCWC 1 cut(s) 2836
BsiHKCI CYCGRG 1 cut(s) 2303
BslFI GGGAC 5 cut(s) 10, 823, 1599, 1754, 2368
BslI CCNNNNNNNGG 7 cut(s) 64, 410, 534, 1116, 2198, 2992, 3047
BsmAI GTCTC 6 cut(s) 242, 283, 582, 788, 1879, 3118
BsmFI GGGAC 5 cut(s) 10, 823, 1599, 1754, 2368
BsmI GAATGC 3 cut(s) 1822, 2746, 3057
BsnI GGCC 7 cut(s) 302, 489, 538, 1063, 2514, 2621, 2731
Bso31I GGTCTC 2 cut(s) 1879, 3118
BsoBI CYCGRG 1 cut(s) 2303
Bsp120I GGGCCC 2 cut(s) 2512, 2619
Bsp1286I GDGCHC 4 cut(s) 2323, 2516, 2623, 2836
Bsp1720I GCTNAGC 1 cut(s) 962
Bsp19I CCATGG 3 cut(s) 45, 297, 2350
BspANI GGCC 7 cut(s) 302, 489, 538, 1063, 2514, 2621, 2731
BspFNI CGCG 1 cut(s) 245
BspHI TCATGA 1 cut(s) 2775
BspOI GCTAGC 1 cut(s) 1427
BspPI GGATC 8 cut(s) 104, 696, 897, 1845, 1858, 2088, 2110, 3159
BspT107I GGYRCC 2 cut(s) 2789, 3037
BspTI CTTAAG 2 cut(s) 427, 514
BspTNI GGTCTC 2 cut(s) 1879, 3118
BsrBI CCGCTC 1 cut(s) 1044
BsrDI GCAATG 1 cut(s) 1434
BsrI ACTGG 9 cut(s) 121, 576, 756, 904, 985, 1129, 2238, 2926, 2984
BssT1I CCWWGG 4 cut(s) 45, 297, 2350, 3085
Bst4CI ACNGT 6 cut(s) 1284, 1356, 2398, 2681, 2696, 2831
BstAFI CTTAAG 2 cut(s) 427, 514
BstBAI YACGTR 1 cut(s) 2654
BstC8I GCNNGC 8 cut(s) 546, 1425, 1959, 2037, 2572, 2788, 2935, 3057
BstDSI CCRYGG 4 cut(s) 45, 243, 297, 2350
BstEII GGTNACC 3 cut(s) 2187, 2696, 2825
BstF5I GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2242, 2476
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 6 cut(s) 242, 283, 582, 788, 1879, 3118
BstMWI GCNNNNNNNGC 9 cut(s) 10, 61, 658, 1429, 2147, 2450, 2530, 2750, 2769
BstNSI RCATGY 2 cut(s) 673, 1987
BstPAI GACNNNNGTC 1 cut(s) 516
BstPI GGTNACC 3 cut(s) 2187, 2696, 2825
BstSFI CTRYAG 4 cut(s) 860, 1335, 2145, 2214
BstSLI GKGCMC 3 cut(s) 2516, 2623, 2836
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 9 cut(s) 64, 66, 152, 549, 560, 2153, 2833, 3016, 3071
BstV2I GAAGAC 1 cut(s) 1933
BstX2I RGATCY 6 cut(s) 688, 889, 1796, 1850, 2080, 3151
BstXI CCANNNNNNTGG 2 cut(s) 1550, 2616
BstYI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2080, 3151
BsuI GTATCC 1 cut(s) 1368
BsuRI GGCC 7 cut(s) 302, 489, 538, 1063, 2514, 2621, 2731
BtgI CCRYGG 4 cut(s) 45, 243, 297, 2350
BtgZI GCGATG 1 cut(s) 2952
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 7 cut(s) 253, 1209, 1263, 1977, 2025, 2242, 2476
BtsI GCAGTG 1 cut(s) 539
BtsIMutI CAGTG 4 cut(s) 114, 539, 2403, 2609
Cac8I GCNNGC 8 cut(s) 546, 1425, 1959, 2037, 2572, 2788, 2935, 3057
CaiI CAGNNNCTG 1 cut(s) 2198
CciI TCATGA 1 cut(s) 2775
CfoI GCGC 1 cut(s) 79
Cfr42I CCGCGG 1 cut(s) 246
CseI GACGC 1 cut(s) 3035
CsiI ACCWGGT 1 cut(s) 3127
Csp6I GTAC 4 cut(s) 805, 1860, 1970, 2503
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviQI GTAC 4 cut(s) 805, 1860, 1970, 2503
DraI TTTAAA 2 cut(s) 1005, 1296
DrdI GACNNNNNNGTC 1 cut(s) 950
DseDI GACNNNNNNGTC 1 cut(s) 950
EaeI YGGCCR 1 cut(s) 300
EciI GGCGGA 1 cut(s) 216
Eco130I CCWWGG 4 cut(s) 45, 297, 2350, 3085
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 3 cut(s) 2323, 2516, 2623
Eco31I GGTCTC 2 cut(s) 1879, 3118
Eco47I GGWCC 6 cut(s) 837, 1259, 1697, 1841, 2983, 3082
Eco57I CTGAAG 4 cut(s) 1502, 1623, 2055, 2349
Eco72I CACGTG 1 cut(s) 2654
Eco88I CYCGRG 1 cut(s) 2303
Eco91I GGTNACC 3 cut(s) 2187, 2696, 2825
EcoO109I RGGNCCY 6 cut(s) 837, 1259, 1697, 2512, 2513, 3082
EcoO65I GGTNACC 3 cut(s) 2187, 2696, 2825
EcoT14I CCWWGG 4 cut(s) 45, 297, 2350, 3085
EcoT38I GRGCYC 3 cut(s) 2323, 2516, 2623
ErhI CCWWGG 4 cut(s) 45, 297, 2350, 3085
FaqI GGGAC 5 cut(s) 10, 823, 1599, 1754, 2368
FauI CCCGC 1 cut(s) 2926
FbaI TGATCA 1 cut(s) 1564
FokI GGATG 7 cut(s) 260, 1216, 1250, 1964, 2012, 2249, 2463
FriOI GRGCYC 3 cut(s) 2323, 2516, 2623
FspBI CTAG 5 cut(s) 1034, 1412, 1424, 1608, 1722
GlaI GCGC 1 cut(s) 78
GsuI CTGGAG 4 cut(s) 104, 1002, 1782, 2220
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 7 cut(s) 302, 489, 538, 1063, 2514, 2621, 2731
HgaI GACGC 1 cut(s) 3035
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 3 cut(s) 1083, 2606, 2644
HindII GTYRAC 3 cut(s) 1083, 2606, 2644
HindIII AAGCTT 2 cut(s) 281, 2572
Hpy166II GTNNAC 9 cut(s) 253, 761, 1031, 1083, 1826, 1972, 2606, 2644, 2834
Hpy8I GTNNAC 9 cut(s) 253, 761, 1031, 1083, 1826, 1972, 2606, 2644, 2834
HpyCH4III ACNGT 6 cut(s) 1284, 1356, 2398, 2681, 2696, 2831
HpyCH4IV ACGT 5 cut(s) 648, 1079, 1822, 2646, 2653
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 61, 658, 1429, 2147, 2450, 2530, 2750, 2769
HpySE526I ACGT 5 cut(s) 648, 1079, 1822, 2646, 2653
HspAI GCGC 1 cut(s) 77
KflI GGGWCCC 1 cut(s) 837
Ksp22I TGATCA 1 cut(s) 1564
KspI CCGCGG 1 cut(s) 246
LmnI GCTCC 6 cut(s) 9, 60, 423, 1631, 2308, 2326
Lsp1109I GCAGC 9 cut(s) 64, 66, 152, 549, 560, 2153, 2833, 3016, 3071
LweI GCATC 4 cut(s) 693, 2485, 2717, 2800
MabI ACCWGGT 1 cut(s) 3127
MaeI CTAG 5 cut(s) 1034, 1412, 1424, 1608, 1722
MaeII ACGT 5 cut(s) 648, 1079, 1822, 2646, 2653
MbiI CCGCTC 1 cut(s) 1044
MflI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2080, 3151
MhlI GDGCHC 4 cut(s) 2323, 2516, 2623, 2836
MlsI TGGCCA 1 cut(s) 302
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 7 cut(s) 155, 195, 244, 1249, 1517, 2204, 2213
MmeI TCCRAC 4 cut(s) 399, 867, 1658, 2703
Mox20I TGGCCA 1 cut(s) 302
MroXI GAANNNNTTC 1 cut(s) 2802
MscI TGGCCA 1 cut(s) 302
MslI CAYNNNNRTG 4 cut(s) 1398, 1455, 1982, 2481
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 3 cut(s) 245, 2075, 2144
MspCI CTTAAG 2 cut(s) 427, 514
Mva1269I GAATGC 3 cut(s) 1822, 2746, 3057
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 9 cut(s) 10, 61, 658, 1429, 2147, 2450, 2530, 2750, 2769
NciI CCSGG 5 cut(s) 330, 535, 1474, 1772, 1848
NcoI CCATGG 3 cut(s) 45, 297, 2350
NheI GCTAGC 1 cut(s) 1423
NmuCI GTSAC 4 cut(s) 715, 1642, 2187, 2209
NspI RCATGY 2 cut(s) 673, 1987
OliI CACNNNNGTG 1 cut(s) 1455
PagI TCATGA 1 cut(s) 2775
PasI CCCWGGG 1 cut(s) 1811
PceI AGGCCT 1 cut(s) 489
PctI GAATGC 3 cut(s) 1822, 2746, 3057
PdmI GAANNNNTTC 1 cut(s) 2802
PfeI GAWTC 7 cut(s) 271, 476, 811, 1013, 1684, 1995, 2803
PflFI GACNNNGTC 1 cut(s) 2981
PflMI CCANNNNNTGG 1 cut(s) 2198
PfoI TCCNGGA 2 cut(s) 1770, 1846
PleI GAGTC 7 cut(s) 154, 194, 243, 1248, 1516, 2203, 2212
PmaCI CACGTG 1 cut(s) 2654
PmlI CACGTG 1 cut(s) 2654
PpsI GAGTC 7 cut(s) 154, 194, 243, 1248, 1516, 2203, 2212
Ppu21I YACGTR 1 cut(s) 2654
PpuMI RGGWCCY 4 cut(s) 837, 1259, 1697, 3082
PshAI GACNNNNGTC 1 cut(s) 516
PshBI ATTAAT 1 cut(s) 1482
PsiI TTATAA 1 cut(s) 1349
Psp5II RGGWCCY 4 cut(s) 837, 1259, 1697, 3082
PspCI CACGTG 1 cut(s) 2654
PspEI GGTNACC 3 cut(s) 2187, 2696, 2825
PspOMI GGGCCC 2 cut(s) 2512, 2619
PspPPI RGGWCCY 4 cut(s) 837, 1259, 1697, 3082
PstNI CAGNNNCTG 1 cut(s) 2198
PsuI RGATCY 6 cut(s) 688, 889, 1796, 1850, 2080, 3151
PsyI GACNNNGTC 1 cut(s) 2981
PvuII CAGCTG 2 cut(s) 2075, 2144
RsaI GTAC 4 cut(s) 806, 1861, 1971, 2504
RsaNI GTAC 4 cut(s) 805, 1860, 1970, 2503
RseI CAYNNNNRTG 4 cut(s) 1398, 1455, 1982, 2481
SacII CCGCGG 1 cut(s) 246
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 7 cut(s) 155, 195, 244, 1249, 1517, 2204, 2213
SduI GDGCHC 4 cut(s) 2323, 2516, 2623, 2836
SexAI ACCWGGT 1 cut(s) 3127
SfaNI GCATC 4 cut(s) 693, 2485, 2717, 2800
SfcI CTRYAG 4 cut(s) 860, 1335, 2145, 2214
Sfr303I CCGCGG 1 cut(s) 246
SgrBI CCGCGG 1 cut(s) 246
SinI GGWCC 6 cut(s) 837, 1259, 1697, 1841, 2983, 3082
SmiMI CAYNNNNRTG 4 cut(s) 1398, 1455, 1982, 2481
SmlI CTYRAG 5 cut(s) 256, 356, 427, 514, 956
SmoI CTYRAG 5 cut(s) 256, 356, 427, 514, 956
SpeI ACTAGT 1 cut(s) 1721
SseBI AGGCCT 1 cut(s) 489
SspI AATATT 1 cut(s) 2716
SspMI CTAG 5 cut(s) 1034, 1412, 1424, 1608, 1722
StuI AGGCCT 1 cut(s) 489
StyI CCWWGG 4 cut(s) 45, 297, 2350, 3085
TaaI ACNGT 6 cut(s) 1284, 1356, 2398, 2681, 2696, 2831
TaiI ACGT 5 cut(s) 651, 1082, 1825, 2649, 2656
TaqI TCGA 9 cut(s) 311, 675, 948, 1172, 1224, 1398, 1671, 2587, 2808
TaqII GACCGA 1 cut(s) 2197
TatI WGTACW 1 cut(s) 804
TauI GCSGC 3 cut(s) 852, 1047, 2863
TfiI GAWTC 7 cut(s) 271, 476, 811, 1013, 1684, 1995, 2803
TscAI CASTG 4 cut(s) 121, 546, 2403, 2616
TseFI GTSAC 4 cut(s) 715, 1642, 2187, 2209
TseI GCWGC 9 cut(s) 52, 79, 165, 548, 562, 2141, 2846, 3029, 3059
Tsp45I GTSAC 4 cut(s) 715, 1642, 2187, 2209
TspDTI ATGAA 7 cut(s) 1566, 1656, 1734, 1884, 1914, 1933, 3089
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 4 cut(s) 121, 546, 2403, 2616
Tth111I GACNNNGTC 1 cut(s) 2981
Van91I CCANNNNNTGG 1 cut(s) 2198
Vha464I CTTAAG 2 cut(s) 427, 514
VneI GTGCAC 1 cut(s) 2832
VpaK11BI GGWCC 6 cut(s) 837, 1259, 1697, 1841, 2983, 3082
VspI ATTAAT 1 cut(s) 1482
XapI RAATTY 4 cut(s) 695, 773, 1087, 1181
XceI RCATGY 2 cut(s) 673, 1987
XmnI GAANNNNTTC 1 cut(s) 2802
XspI CTAG 5 cut(s) 1034, 1412, 1424, 1608, 1722
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.