RLG00000032249

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0007159
Physical Location & Seq
Forward (+)
12582022 .. 12585524
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032249

Sequence Viewer

Length: 3150 bp
ATGGGACAGCTTCCTTCGCAGGATATATTGGCACTGCTCGCGTTCAAGAAAGGTGTCAAGCATGATCCTACTGGTTTTGTTGTCAATTCTTGGAATGATGAGTCCATTGACTTCAATGGTTGTCCTTCTTCTTGGAATGGGGTTATATGTAATGGTGGAAATGTTGCTGGAGTTGTCCTTGATAATTTGAGTCTCTCTGCGGATGTGGACTTGAGTGTGTTTTCGAATCTAACCAAGCTTCTGAAACTTTCCATGGCCAACAATTCGATATCTGGGAAAATTCCTGACAATATTGCAGACTTCAACAGCCTCCAGTTTCTTGATCTCTCCAACAATTTATTTGCTTCATCTTTACCACCTGGAATTGGTAAGTTGGGGAGCTTAAGGAACCTCTCATTAGCTGGCAATAACTTCTCGGGCTCCATTCCAGATTCCATTTCAGGGCTTTCCTCAATCCAGTCGTTGGACTTAAGTCGCAACTCCTTTTCAGGGCCGCTGCCGTCCGCATTGACAAAGCTGTCCAGTCTGGTATCTCTAAATCTTTCTTTGAATGGCTTTACCAAGAGCTTACCTAAGGGGTTTGAGCTAATGTCAAGTCTAGATGTGCTCGACTTGCATGGAAATATGCTTGATGGTCCTCTGGATAAGGCATTTCTGATGGAAGCAACTGCCACGCATGTTGATTTTAGTAGCAATATGTTTACAAGTTCCAGTTCACAGCAGCAGATGTTTCTACCTCGTCTTTCTGAGAGTATCAAGTACTTGAATCTTTCCCACAACCAGCTTACTGGGTCACTGGTAGGTGGCAGTGAGCTTCAGATATTTGAGGACTTGAAGGTGTTGGATCTCAGCTACAACCAGCTGTCAGGAGAATTGCCTGGATTTAATTTTGTCTATGACCTCCAGGTCCTCAAACTTAGCAACAACAGATTTACTGGAGTTGTTCCCAATGGCCTAATAAAAGGAGATTCTCTGGTTTTAAGTGAACTAGATCTGAGTGGCAACAATCTCTCAGGACCAATAACTATGGTGACATCAACAACTCTGAGCATCCTTAATCTCTCCTCGAATGGGCTTACTGGTATGTTGCCATTGCTGACTGGAACTTGTGCTGTACTTGATCTATCCAAGAACAAATTAGAGGGAAATTTAACCAGGATGGTTAAATGGGGGAACATTGAATACCTTGATCTGAGTCAAAATCTTTTGACAGGACCCATCCCTGATGTCACTCCACAATTTTTGCGTTTAAATTATCTCAATCTCTCCCATAATTCTCTTAGTAGTTCCATAGCAAGTGTTATAACACAGTATCCAAAGATTAGTGTCCTTGATCTCAGTTCTAACCAGTTAGATGGCACAGTTCTAGCTGAGTTGCTAAGCATGCCTACTCTGCAAGAGCTCCACCTTGAAAATAATTTACTCACTGGGTCTATCAACATTTCATCTCCTTTGTTCAACCAATCCAATCTTCAGGTTCTTGATCTTTCTCAGAACCGGCTGAGTGGTTATTTTCCTGATCAATTTGGTTCACTGAATGGGCTTAAAGTGCTCAATATTGGGAGAAATAATCTTTCTGGCTCTCTGCCAACTTCCATATCTGACATGAGCACACTAAGCTCATTAGACATATCGCAGAATCATTTCACAGGTCCTCTTCCAAACAACTTGCCAAACAGTCTTGTATTCTTTAATGCTTCATATAATGATCTTTCTGGGGATGTCCCAGAAAATCTGAGAAAGTTTCCGAGTTCTTCCTTCTATCCAGGGAATACACGCTTGCGGTTTCCAAGTGGTGGTGGTCCTGGATCAAACAATTCCGAATCTGAACATTCAAAGAGGAAACCATTCAGCACACTCGTCAAAGTTATCATTATAGTTTCGTGTGTGGTTGCTGTTTTCATTCTTCTTCTGCTTGGTATCTTCATTCATTACATCTGCATGTCAAGGAGAACCCCATCAGAGAATACTGCAAACAAAGACATCCACAAACGTGCTCCACCAAACCCCTCAGGTGCTCGAGGAGCAGAGAGTGGTGGTGCTTTGGTTGTTTCAGCTGGGGATCTTGTGGCTTCACGGAAAGGATCGTCTTCTGAAATTATCAGCCCTGGTGAAAAAGTCGCAGCTGTAAGTGACTTCTCCCCTTCAAAGAACAGCCATTTCTCTTGGTCACCAGAGTCTGGAGATTCCTACACTGCGGAAAACCTTGCCAGACTGGACGTGAGATCACCGGATAGATTGGTTGGTGAGCTTCATTTTCTTGATGAAACAATTGCATTAACACCTGAGGAGCTTTCAAGGGCTCCTGCTGAAGTTTTGGGGAGAAGCAGCCATGGCACTTCTTATAAGGCAACACTCGATAACGGGCTGTTCTTGACAGTTAAGTGGTTGAGAGAAGGGGTGGCAAAGCAGAAAAAGGAGTTTGCCAAGGAGGCTAAGAAATTTGCCAATATGAGGCATCCAAATGTTGTGGGTTTGAGAGGGTACTATTGGGGTCCTACACAGCATGAGAAGCTTATTCTTTCAGATTATATCTCACCTGGAAGTCTTGCAAGCTTTCTTTATGATCGACCAGGAAGAAAAGGTCCACCATTGACCTGGGCCCAAAGGCTCAAAATTGCAGTTGATGTTGCACGTGGCCTGAACTATCTCCATTTTGATCGTGCTGTTCCGCACGGTAACCTCAAAGCAACAAATATTCTGTTGGATGGGTCTGATCTGAATGCCCGAGTTGCTGATTACTGCCTCCACCGCCTCATGACACAAGCTGGCACCATCGAACAGATTCTTGATGCTGGTGTCTTAGGTTACCGCGCCCCAGAGCTGGCTGCTTCCAAGAAGCCACTGCCCTCCTTCAAGTCCGATGTTTACGCCTTTGGAGTGATATTGTTGGAACTTTTAACTGGAAGGTGCGCTGGTGATGTGATCTCTGGTGAAGGAGGCGGGGCAGATTTGACAGAATGGGTGCGGTTGAGGGTAGCAGAAGGTCGTGGGTCAGATTGTTTTGATACTGCATTGGTGACTGAGATGGGAAATCCAGCAGCAGAGAAGGGAATGAAGGAAGTCCTTGGAATATCATTACGGTGTATACGTTCTGTTTCTGAGAGGCCAGGTATTAAGACTATATATGAGGATCTCTCTTCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1050

Amino Acids

112.94

Weight (kDa)

6.03

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 6 - 51 2.5e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 68 - 113 8.1e-06 Leucine rich repeat
LRR_14 PF23598 122 - 206 2.4e-08 Leucine-rich repeat region
LRR_8 PF13855 489 - 549 2.5e-11 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 771 - 968 4.3e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 771 - 970 1.2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1304, 2346
AasI GACNNNNNNGTC 2 cut(s) 517, 905
AccB1I GGYRCC 1 cut(s) 2771
AccB7I CCANNNNNTGG 3 cut(s) 463, 1796, 2180
AccI GTMKAC 1 cut(s) 3088
AccII CGCG 2 cut(s) 41, 2814
AclWI GGATC 6 cut(s) 59, 852, 1816, 2070, 2092, 3141
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 3 cut(s) 279, 1147, 2441
AcuI CTGAAG 3 cut(s) 800, 1457, 2331
AcvI CACGTG 1 cut(s) 2636
AfaI GTAC 3 cut(s) 761, 1116, 2486
AfiI CCNNNNNNNGG 9 cut(s) 365, 441, 463, 489, 1071, 1796, 2180, 2454, 2824
AflII CTTAAG 2 cut(s) 382, 469
AjiI CACGTC 1 cut(s) 2221
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
AleI CACNNNNGTG 1 cut(s) 1992
Alw21I GWGCWC 6 cut(s) 609, 1404, 1554, 1613, 1999, 2020
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 6 cut(s) 59, 852, 1816, 2070, 2092, 3141
AlwNI CAGNNNCTG 1 cut(s) 2180
Ama87I CYCGRG 3 cut(s) 415, 2019, 2727
AoxI GGCC 6 cut(s) 255, 491, 952, 2601, 2638, 3107
ApaI GGGCCC 1 cut(s) 2605
ApeKI GCWGC 6 cut(s) 496, 721, 2123, 2328, 2828, 3041
ApoI RAATTY 3 cut(s) 279, 1147, 2441
Asp700I GAANNNNTTC 3 cut(s) 1643, 1847, 2784
AspLEI GCGC 2 cut(s) 2816, 2915
AsuHPI GGTGA 9 cut(s) 1042, 2123, 2163, 2220, 2258, 2529, 2930, 2945, 3031
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 3 cut(s) 415, 2019, 2727
AvaII GGWCC 8 cut(s) 635, 907, 1016, 1214, 1652, 1802, 2495, 2585
AxyI CCTNAGG 3 cut(s) 573, 2011, 2286
BaeGI GKGCMC 1 cut(s) 2605
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 2771
BanII GRGCYC 4 cut(s) 422, 1404, 2305, 2605
BbrPI CACGTG 1 cut(s) 2636
BbsI GAAGAC 1 cut(s) 2082
Bbv12I GWGCWC 6 cut(s) 609, 1404, 1554, 1613, 1999, 2020
BbvI GCAGC 6 cut(s) 483, 733, 2135, 2340, 2815, 3053
BccI CCATC 9 cut(s) 626, 652, 1153, 1226, 1349, 1966, 2702, 2783, 3022
BceAI ACGGC 1 cut(s) 484
BciVI GTATCC 1 cut(s) 1323
BclI TGATCA 1 cut(s) 1519
BcoDI GTCTC 1 cut(s) 197
BfaI CTAG 3 cut(s) 599, 989, 1367
BfrI CTTAAG 2 cut(s) 382, 469
BfuI GTATCC 1 cut(s) 1323
BglI GCCNNNNNGGC 1 cut(s) 2432
BglII AGATCT 1 cut(s) 991
BisI GCNGC 7 cut(s) 494, 497, 722, 2124, 2329, 2829, 3042
BlpI GCTNAGC 1 cut(s) 1379
BlsI GCNGC 7 cut(s) 495, 498, 723, 2125, 2330, 2830, 3043
BmcAI AGTACT 1 cut(s) 761
Bme18I GGWCC 8 cut(s) 635, 907, 1016, 1214, 1652, 1802, 2495, 2585
BmeT110I CYCGRG 3 cut(s) 415, 2019, 2727
BmgBI CACGTC 1 cut(s) 2221
BmiI GGNNCC 7 cut(s) 389, 421, 1216, 2304, 2496, 2603, 2773
BmrI ACTGGG 2 cut(s) 798, 1437
BmsI GCATC 3 cut(s) 1059, 2467, 2782
BmuI ACTGGG 2 cut(s) 798, 1437
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 2082
BplI GAGNNNNNCTC 1 cut(s) 3122
BpmI CTGGAG 5 cut(s) 189, 296, 887, 957, 2202
Bpu1102I GCTNAGC 1 cut(s) 1379
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 232
BsaAI YACGTR 1 cut(s) 2636
BsaJI CCNNGG 7 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067
BsaWI WCCGGW 1 cut(s) 2230
Bsc4I CCNNNNNNNGG 9 cut(s) 365, 441, 463, 489, 1071, 1796, 2180, 2454, 2824
Bse118I RCCGGY 1 cut(s) 1497
Bse21I CCTNAGG 3 cut(s) 573, 2011, 2286
Bse3DI GCAATG 1 cut(s) 1091
BseDI CCNNGG 7 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067
BseGI GGATG 8 cut(s) 208, 1050, 1164, 1218, 1726, 1983, 2458, 2713
BseLI CCNNNNNNNGG 9 cut(s) 365, 441, 463, 489, 1071, 1796, 2180, 2454, 2824
BseMI GCAATG 1 cut(s) 1091
BseRI GAGGAG 3 cut(s) 1054, 2037, 2303
BseSI GKGCMC 1 cut(s) 2605
BseXI GCAGC 6 cut(s) 483, 733, 2135, 2340, 2815, 3053
BseYI CCCAGC 1 cut(s) 2057
Bsh1236I CGCG 2 cut(s) 41, 2814
BshFI GGCC 6 cut(s) 257, 493, 954, 2603, 2640, 3109
BshNI GGYRCC 1 cut(s) 2771
BsiHKAI GWGCWC 6 cut(s) 609, 1404, 1554, 1613, 1999, 2020
BsiHKCI CYCGRG 3 cut(s) 415, 2019, 2727
BsiSI CCGG 2 cut(s) 1498, 2231
BslFI GGGAC 2 cut(s) 18, 1709
BslI CCNNNNNNNGG 9 cut(s) 365, 441, 463, 489, 1071, 1796, 2180, 2454, 2824
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 18, 1709
BsmI GAATGC 1 cut(s) 2728
BsnI GGCC 6 cut(s) 257, 493, 954, 2603, 2640, 3109
BsoBI CYCGRG 3 cut(s) 415, 2019, 2727
Bsp119I TTCGAA 1 cut(s) 224
Bsp120I GGGCCC 1 cut(s) 2601
Bsp1286I GDGCHC 9 cut(s) 422, 609, 1404, 1554, 1613, 1999, 2020, 2305, 2605
Bsp1720I GCTNAGC 1 cut(s) 1379
Bsp19I CCATGG 2 cut(s) 252, 2332
BspANI GGCC 6 cut(s) 257, 493, 954, 2603, 2640, 3109
BspFNI CGCG 2 cut(s) 41, 2814
BspHI TCATGA 1 cut(s) 2757
BspLI GGNNCC 7 cut(s) 389, 421, 1216, 2304, 2496, 2603, 2773
BspPI GGATC 6 cut(s) 59, 852, 1816, 2070, 2092, 3141
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 2771
BspTI CTTAAG 2 cut(s) 382, 469
BsrDI GCAATG 1 cut(s) 1091
BsrFI RCCGGY 1 cut(s) 1497
BssAI RCCGGY 1 cut(s) 1497
BssECI CCNNGG 7 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067
BssNAI GTATAC 1 cut(s) 3089
BssT1I CCWWGG 4 cut(s) 252, 2332, 2427, 3067
Bst1107I GTATAC 1 cut(s) 3089
Bst4CI ACNGT 6 cut(s) 1311, 1363, 1679, 2380, 2678, 3084
Bst6I CTCTTC 2 cut(s) 1662, 3145
BstAFI CTTAAG 2 cut(s) 382, 469
BstBAI YACGTR 1 cut(s) 2636
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 7 cut(s) 39, 403, 1385, 1781, 2554, 2770, 2826
BstDSI CCRYGG 2 cut(s) 252, 2332
BstEII GGTNACC 3 cut(s) 2169, 2678, 2807
BstF5I GGATG 8 cut(s) 208, 1050, 1164, 1218, 1726, 1983, 2458, 2713
BstFNI CGCG 2 cut(s) 41, 2814
BstHHI GCGC 2 cut(s) 2816, 2915
BstMAI GTCTC 1 cut(s) 197
BstNSI RCATGY 3 cut(s) 680, 1387, 1945
BstPAI GACNNNNGTC 1 cut(s) 471
BstPI GGTNACC 3 cut(s) 2169, 2678, 2807
BstSLI GKGCMC 1 cut(s) 2605
BstUI CGCG 2 cut(s) 41, 2814
BstV1I GCAGC 6 cut(s) 483, 733, 2135, 2340, 2815, 3053
BstV2I GAAGAC 1 cut(s) 2082
BstX2I RGATCY 4 cut(s) 844, 991, 2062, 3133
BstXI CCANNNNNNTGG 3 cut(s) 788, 1355, 2598
BstYI RGATCY 4 cut(s) 844, 991, 2062, 3133
BstZ17I GTATAC 1 cut(s) 3089
Bsu36I CCTNAGG 3 cut(s) 573, 2011, 2286
BsuI GTATCC 1 cut(s) 1323
BsuRI GGCC 6 cut(s) 257, 493, 954, 2603, 2640, 3109
BtgI CCRYGG 2 cut(s) 252, 2332
BtrI CACGTC 1 cut(s) 2221
BtsCI GGATG 8 cut(s) 208, 1050, 1164, 1218, 1726, 1983, 2458, 2713
BtsI GCAGTG 4 cut(s) 32, 814, 2193, 2843
BtsIMutI CAGTG 7 cut(s) 32, 794, 814, 1425, 1532, 2193, 2843
Cac8I GCNNGC 7 cut(s) 39, 403, 1385, 1781, 2554, 2770, 2826
CaiI CAGNNNCTG 1 cut(s) 2180
CciI TCATGA 1 cut(s) 2757
CfoI GCGC 2 cut(s) 2816, 2915
Cfr10I RCCGGY 1 cut(s) 1497
Csp6I GTAC 3 cut(s) 760, 1115, 2485
CspCI CAANNNNNGTGG 6 cut(s) 661, 696, 1224, 1259, 2451, 2486
CviQI GTAC 3 cut(s) 760, 1115, 2485
DraI TTTAAA 1 cut(s) 1251
DrdI GACNNNNNNGTC 2 cut(s) 517, 905
DseDI GACNNNNNNGTC 2 cut(s) 517, 905
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 2 cut(s) 1662, 3145
EarI CTCTTC 2 cut(s) 1662, 3145
Ecl136II GAGCTC 1 cut(s) 1402
Eco130I CCWWGG 4 cut(s) 252, 2332, 2427, 3067
Eco24I GRGCYC 4 cut(s) 422, 1404, 2305, 2605
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 8 cut(s) 635, 907, 1016, 1214, 1652, 1802, 2495, 2585
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 3 cut(s) 800, 1457, 2331
Eco72I CACGTG 1 cut(s) 2636
Eco81I CCTNAGG 3 cut(s) 573, 2011, 2286
Eco88I CYCGRG 3 cut(s) 415, 2019, 2727
Eco91I GGTNACC 3 cut(s) 2169, 2678, 2807
EcoICRI GAGCTC 1 cut(s) 1402
EcoO109I RGGNCCY 4 cut(s) 907, 1214, 1652, 2495
EcoO65I GGTNACC 3 cut(s) 2169, 2678, 2807
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 4 cut(s) 252, 2332, 2427, 3067
EcoT38I GRGCYC 4 cut(s) 422, 1404, 2305, 2605
ErhI CCWWGG 4 cut(s) 252, 2332, 2427, 3067
FaqI GGGAC 2 cut(s) 18, 1709
FauI CCCGC 1 cut(s) 2936
FbaI TGATCA 1 cut(s) 1519
FblI GTMKAC 1 cut(s) 3088
Fnu4HI GCNGC 7 cut(s) 494, 497, 722, 2124, 2329, 2829, 3042
FokI GGATG 8 cut(s) 215, 1037, 1171, 1205, 1733, 1970, 2445, 2720
FriOI GRGCYC 4 cut(s) 422, 1404, 2305, 2605
Fsp4HI GCNGC 7 cut(s) 494, 497, 722, 2124, 2329, 2829, 3042
FspBI CTAG 3 cut(s) 599, 989, 1367
GlaI GCGC 2 cut(s) 2815, 2914
GluI GCNGC 7 cut(s) 494, 497, 722, 2124, 2329, 2829, 3042
GsaI CCCAGC 1 cut(s) 2061
GsuI CTGGAG 5 cut(s) 189, 296, 887, 957, 2202
HaeIII GGCC 6 cut(s) 257, 493, 954, 2603, 2640, 3109
HapII CCGG 2 cut(s) 1498, 2231
HhaI GCGC 2 cut(s) 2816, 2915
Hin6I GCGC 2 cut(s) 2814, 2913
HinP1I GCGC 2 cut(s) 2814, 2913
HindIII AAGCTT 3 cut(s) 236, 2513, 2554
HpaII CCGG 2 cut(s) 1498, 2231
HphI GGTGA 9 cut(s) 1042, 2123, 2163, 2220, 2258, 2529, 2930, 2945, 3031
Hpy166II GTNNAC 8 cut(s) 208, 702, 716, 986, 1532, 2588, 2869, 3089
Hpy8I GTNNAC 8 cut(s) 208, 702, 716, 986, 1532, 2588, 2869, 3089
HpyCH4III ACNGT 6 cut(s) 1311, 1363, 1679, 2380, 2678, 3084
HpyCH4IV ACGT 4 cut(s) 1993, 2220, 2635, 3091
HpySE526I ACGT 4 cut(s) 1993, 2220, 2635, 3091
HspAI GCGC 2 cut(s) 2814, 2913
Ksp22I TGATCA 1 cut(s) 1519
LmnI GCTCC 7 cut(s) 378, 425, 1407, 2002, 2024, 2290, 2308
Lsp1109I GCAGC 6 cut(s) 483, 733, 2135, 2340, 2815, 3053
LweI GCATC 3 cut(s) 1059, 2467, 2782
MaeI CTAG 3 cut(s) 599, 989, 1367
MaeII ACGT 4 cut(s) 1993, 2220, 2635, 3091
MaeIII GTNAC 8 cut(s) 792, 1030, 1228, 2132, 2169, 2678, 2807, 3019
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 4 cut(s) 844, 991, 2062, 3133
MhlI GDGCHC 9 cut(s) 422, 609, 1404, 1554, 1613, 1999, 2020, 2305, 2605
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 4 cut(s) 110, 199, 1204, 2186
MmeI TCCRAC 5 cut(s) 354, 444, 822, 2685, 2871
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 3 cut(s) 1643, 1847, 2784
MscI TGGCCA 1 cut(s) 257
MslI CAYNNNNRTG 4 cut(s) 1940, 1992, 2463, 3082
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 4 cut(s) 496, 862, 2057, 2126
MspCI CTTAAG 2 cut(s) 382, 469
MspI CCGG 2 cut(s) 1498, 2231
MunI CAATTG 1 cut(s) 2271
Mva1269I GAATGC 1 cut(s) 2728
MvnI CGCG 2 cut(s) 41, 2814
NcoI CCATGG 2 cut(s) 252, 2332
NlaIV GGNNCC 7 cut(s) 389, 421, 1216, 2304, 2496, 2603, 2773
NmuCI GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
NspI RCATGY 3 cut(s) 680, 1387, 1945
NspV TTCGAA 1 cut(s) 224
OliI CACNNNNGTG 1 cut(s) 1992
PaeI GCATGC 1 cut(s) 1387
PaeR7I CTCGAG 1 cut(s) 2019
PagI TCATGA 1 cut(s) 2757
PcsI WCGNNNNNNNCGW 1 cut(s) 3088
PctI GAATGC 1 cut(s) 2728
PdmI GAANNNNTTC 3 cut(s) 1643, 1847, 2784
PfeI GAWTC 8 cut(s) 226, 431, 766, 968, 1639, 1823, 2186, 2785
PflMI CCANNNNNTGG 3 cut(s) 463, 1796, 2180
PfoI TCCNGGA 1 cut(s) 1804
PkrI GCNGC 7 cut(s) 495, 498, 723, 2125, 2330, 2830, 3043
PleI GAGTC 4 cut(s) 109, 198, 1203, 2185
PmaCI CACGTG 1 cut(s) 2636
PmlI CACGTG 1 cut(s) 2636
PpsI GAGTC 4 cut(s) 109, 198, 1203, 2185
Ppu21I YACGTR 1 cut(s) 2636
PpuMI RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PshAI GACNNNNGTC 1 cut(s) 471
PsiI TTATAA 2 cut(s) 1304, 2346
Psp124BI GAGCTC 1 cut(s) 1404
Psp5II RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PspCI CACGTG 1 cut(s) 2636
PspEI GGTNACC 3 cut(s) 2169, 2678, 2807
PspFI CCCAGC 1 cut(s) 2057
PspN4I GGNNCC 7 cut(s) 389, 421, 1216, 2304, 2496, 2603, 2773
PspOMI GGGCCC 1 cut(s) 2601
PspPPI RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PspXI VCTCGAGB 1 cut(s) 2019
PsrI GAACNNNNNNTAC 2 cut(s) 1166, 1198
PstNI CAGNNNCTG 1 cut(s) 2180
PsuI RGATCY 4 cut(s) 844, 991, 2062, 3133
PvuII CAGCTG 3 cut(s) 862, 2057, 2126
RsaI GTAC 3 cut(s) 761, 1116, 2486
RsaNI GTAC 3 cut(s) 760, 1115, 2485
RseI CAYNNNNRTG 4 cut(s) 1940, 1992, 2463, 3082
SacI GAGCTC 1 cut(s) 1404
SatI GCNGC 7 cut(s) 494, 497, 722, 2124, 2329, 2829, 3042
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 4 cut(s) 110, 199, 1204, 2186
SduI GDGCHC 9 cut(s) 422, 609, 1404, 1554, 1613, 1999, 2020, 2305, 2605
SfaNI GCATC 3 cut(s) 1059, 2467, 2782
Sfr274I CTCGAG 1 cut(s) 2019
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 8 cut(s) 635, 907, 1016, 1214, 1652, 1802, 2495, 2585
SlaI CTCGAG 1 cut(s) 2019
SmiMI CAYNNNNRTG 4 cut(s) 1940, 1992, 2463, 3082
SmlI CTYRAG 4 cut(s) 211, 382, 469, 2019
SmoI CTYRAG 4 cut(s) 211, 382, 469, 2019
SphI GCATGC 1 cut(s) 1387
SspI AATATT 3 cut(s) 292, 1558, 2698
SspMI CTAG 3 cut(s) 599, 989, 1367
SstI GAGCTC 1 cut(s) 1404
StyI CCWWGG 4 cut(s) 252, 2332, 2427, 3067
TaaI ACNGT 6 cut(s) 1311, 1363, 1679, 2380, 2678, 3084
TaiI ACGT 4 cut(s) 1996, 2223, 2638, 3094
TaqI TCGA 8 cut(s) 224, 266, 609, 1067, 2020, 2358, 2569, 2778
TatI WGTACW 2 cut(s) 759, 1114
TauI GCSGC 1 cut(s) 496
TfiI GAWTC 8 cut(s) 226, 431, 766, 968, 1639, 1823, 2186, 2785
TscAI CASTG 7 cut(s) 39, 801, 814, 1432, 1539, 2200, 2850
TseFI GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
TseI GCWGC 6 cut(s) 496, 721, 2123, 2328, 2828, 3041
Tsp45I GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
TspDTI ATGAA 9 cut(s) 336, 1434, 1689, 1891, 1915, 1919, 2243, 2280, 3071
TspGWI ACGGA 1 cut(s) 2092
TspRI CASTG 7 cut(s) 39, 801, 814, 1432, 1539, 2200, 2850
Van91I CCANNNNNTGG 3 cut(s) 463, 1796, 2180
Vha464I CTTAAG 2 cut(s) 382, 469
VpaK11BI GGWCC 8 cut(s) 635, 907, 1016, 1214, 1652, 1802, 2495, 2585
XapI RAATTY 3 cut(s) 279, 1147, 2441
XbaI TCTAGA 1 cut(s) 598
XceI RCATGY 3 cut(s) 680, 1387, 1945
XcmI CCANNNNNNNNNTGG 1 cut(s) 2595
XhoI CTCGAG 1 cut(s) 2019
XmiI GTMKAC 1 cut(s) 3088
XmnI GAANNNNTTC 3 cut(s) 1643, 1847, 2784
XspI CTAG 3 cut(s) 599, 989, 1367
ZrmI AGTACT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.