Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010760

Physical Location & Seq

Genomic Coordinates Reverse (-)

41438754 .. 41441672

Gene Structure 2919 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1280400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGGCGTCGAGGTCGAACACCGGAAGCAGGACGCGGGTCGGGCGGTACGAGCTGGGTCGGACTCTCGGGGAGGGCAATTTCGCCAAGGTCAAGTTCGCTCGCAACGTCGAGACAGGGGAGAACTTCGCGATCAAAGTTCTCGACAAGGAGAAGGTCCTCAAGCACAAGATGATCGGCCAGATCAAACGAGAAATATCAACAATGAAATTAATTAGACATCCGAATGTCATCCGCATGTATGAGGTGATGGCTAGCAAGACGAAAATATACATTGTTTTGGAATTTGTGACTGGTGGAGAACTATTTGACAAAATTGCAAGTAAAGGAAGATTGAAAGAAGATGAAGCAAGAAAGTATTTTCAGCAGCTTATAAATGCTGTTGATTACTGTCATAGCAGAGGTGTTTTTCATAGGGACCTAAAGCCAGAGAATTTGCTGCTAGATGTTAGCGGGACTCTTAAAGTTTCAGATTTTGGATTGAGTGCCCTACCTCAGCAAGTGCGAGAAGATGGATTGCTTCACACAACATGTGGAACACCAAATTACGTTGCCCCTGAGGTAATCAACAACAAAGGGTATGATGGAGCGAAAGCAGATTTATGGTCTTGTGGTGTGATTCTTTTCGTCTTAATGGCTGGGTATTTGCCTTTTGAAGATTCCAACCTCATGTCATTATACAAAAAGATATTTAAGGCTGAGTTCAAATGTCCTCCATGGTTCTCCTCGAGTGCAAAGAAGCTAATCAAGAGAATCTTGGATCCTAACCCCATGACAAGAATTACAATTGCTGAGGTCATTGAGAATGAGTGGTTTAAGAAAGGATATAAGCCACCTAGTTTTGAACAAGTTGATGTCAGTCTTGATGATGTGGATTCTATATTCAATGAATCAGGGGATTCTCAGAACCTTGTAGTGGAGAGGCGAGAAGAGCGGAATGTGGCACCTGTCACAATGAATGCCTTTGAGCTTATCTCTACATCCCAGGGCCTGAATCTCAATAGTCTCTTTGAGAAACAAATGGTATGGTTAAATTTGAAAAACAAACGTAAAATTGTAATTGTTCTATATCATGCTGTTGTTTTTCTTTGCTTAATTGGCAAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0007154 GO:0007165 GO:0007584 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009536 GO:0009605 GO:0009628 GO:0009987 GO:0009991 GO:0010035 GO:0010107 GO:0010118 GO:0015672 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0030001 GO:0031667 GO:0034220 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0065007 GO:0071704 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

369

Amino Acids

41.83

Weight (kDa)

9.25

Isoelectric Point (pI)

30.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	16 - 271	1.7e-77	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	17 - 267	5.9e-39	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	106 - 254	2.3e-06	Kinase-like
NAF	PF03822	315 - 339	9.1e-09	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30270 AT1G30270 AT1G30270
fragaria_vesca	FvH4_3g05110 FvH4_3g05110 FvH4_3g05110
malus_domestica	MD05G1280400.v1.1 MD10G1258700.v1.1
prunus_persica	Prupe.4G085000_v2.0.a1 Prupe.4G085000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0014761
rosa_laevigata	RLG00000032138
rosa_multiflora	Rmu_co8519507.1_g000001 Rmu_sc0017371.1_g000001
rosa_roxburghii	Rroxscaffold_1G00061780 Rroxscaffold_3G00222800
rosa_rugosa	Rorug05G0020500
rosa_samantha	Rh5AG116100 Rh5BG112900
rosa_wichuraiana	Rw5G010020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	371
AccB1I	GGYRCC	1 cut(s)	940
AccBSI	CCGCTC	1 cut(s)	931
AccII	CGCG	2 cut(s)	34, 128
AciI	CCGC	5 cut(s)	34, 43, 232, 450, 931
AclWI	GGATC	2 cut(s)	752, 765
AcoI	YGGCCR	1 cut(s)	175
AcsI	RAATTY	3 cut(s)	281, 430, 1030
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	47
AfiI	CCNNNNNNNGG	2 cut(s)	27, 913
AflIII	ACRYGT	1 cut(s)	527
AgsI	TTSAA	6 cut(s)	334, 653, 703, 842, 883, 1036
AjnI	CCWGG	1 cut(s)	981
AjuI	GAANNNNNNNTTGG	2 cut(s)	77, 109
AluBI	AGCT	4 cut(s)	52, 367, 739, 967
AluI	AGCT	4 cut(s)	52, 367, 739, 967
Alw26I	GTCTC	2 cut(s)	104, 1007
AlwI	GGATC	2 cut(s)	752, 765
AlwNI	CAGNNNCTG	1 cut(s)	988
Ama87I	CYCGRG	2 cut(s)	65, 724
AoxI	GGCC	2 cut(s)	175, 985
ApeKI	GCWGC	2 cut(s)	364, 436
ApoI	RAATTY	3 cut(s)	281, 430, 1030
AseI	ATTAAT	1 cut(s)	209
AspS9I	GGNCC	3 cut(s)	154, 415, 985
AsuHPI	GGTGA	1 cut(s)	256
AsuNHI	GCTAGC	1 cut(s)	251
AvaI	CYCGRG	2 cut(s)	65, 724
AvaII	GGWCC	2 cut(s)	154, 415
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	1 cut(s)	487
BamHI	GGATCC	1 cut(s)	757
BanI	GGYRCC	1 cut(s)	940
BbvCI	CCTCAGC	2 cut(s)	492, 789
BbvI	GCAGC	2 cut(s)	376, 423
BccI	CCATC	3 cut(s)	241, 503, 575
BciT130I	CCWGG	1 cut(s)	983
BcoDI	GTCTC	2 cut(s)	104, 1007
BfaI	CTAG	3 cut(s)	252, 440, 834
BisI	GCNGC	2 cut(s)	365, 437
BlsI	GCNGC	2 cut(s)	366, 438
Bme1390I	CCNGG	1 cut(s)	983
Bme18I	GGWCC	2 cut(s)	154, 415
BmeT110I	CYCGRG	2 cut(s)	65, 724
BmgT120I	GGNCC	3 cut(s)	154, 415, 985
BmiI	GGNNCC	3 cut(s)	416, 759, 942
BmrFI	CCNGG	1 cut(s)	983
BmtI	GCTAGC	1 cut(s)	255
BoxI	GACNNNNGTC	1 cut(s)	35
BplI	GAGNNNNNCTC	2 cut(s)	956, 988
Bpu10I	CCTNAGC	2 cut(s)	492, 789
BpuEI	CTTGAG	1 cut(s)	143
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	4 cut(s)	84, 713, 981, 982
BsaWI	WCCGGW	1 cut(s)	20
Bsc4I	CCNNNNNNNGG	2 cut(s)	27, 913
Bse1I	ACTGG	1 cut(s)	295
Bse21I	CCTNAGG	1 cut(s)	555
BseBI	CCWGG	1 cut(s)	983
BseDI	CCNNGG	4 cut(s)	84, 713, 981, 982
BseGI	GGATG	3 cut(s)	217, 228, 977
BseLI	CCNNNNNNNGG	2 cut(s)	27, 913
BseMII	CTCAG	5 cut(s)	506, 546, 687, 780, 914
BseNI	ACTGG	1 cut(s)	295
BseRI	GAGGAG	1 cut(s)	712
BseSI	GKGCMC	1 cut(s)	487
BseXI	GCAGC	2 cut(s)	376, 423
BseYI	CCCAGC	2 cut(s)	52, 635
Bsh1236I	CGCG	2 cut(s)	34, 128
BshFI	GGCC	2 cut(s)	177, 987
BshNI	GGYRCC	1 cut(s)	940
BsiHKCI	CYCGRG	2 cut(s)	65, 724
BsiSI	CCGG	1 cut(s)	21
BslFI	GGGAC	2 cut(s)	428, 466
BslI	CCNNNNNNNGG	2 cut(s)	27, 913
BsmAI	GTCTC	2 cut(s)	104, 1007
BsmFI	GGGAC	2 cut(s)	428, 466
BsmI	GAATGC	1 cut(s)	961
BsnI	GGCC	2 cut(s)	177, 987
BsoBI	CYCGRG	2 cut(s)	65, 724
Bsp1286I	GDGCHC	1 cut(s)	487
Bsp143I	GATC	4 cut(s)	129, 171, 180, 757
Bsp19I	CCATGG	1 cut(s)	713
Bsp68I	TCGCGA	1 cut(s)	128
BspACI	CCGC	5 cut(s)	34, 43, 232, 450, 931
BspANI	GGCC	2 cut(s)	177, 987
BspCNI	CTCAG	5 cut(s)	505, 547, 688, 781, 913
BspFNI	CGCG	2 cut(s)	34, 128
BspLI	GGNNCC	3 cut(s)	416, 759, 942
BspOI	GCTAGC	1 cut(s)	255
BspPI	GGATC	2 cut(s)	752, 765
BspQI	GCTCTTC	1 cut(s)	921
BspT107I	GGYRCC	1 cut(s)	940
BsrBI	CCGCTC	1 cut(s)	931
BsrI	ACTGG	1 cut(s)	295
BssECI	CCNNGG	4 cut(s)	84, 713, 981, 982
BssMI	GATC	4 cut(s)	129, 171, 180, 757
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	84, 713
Bst2UI	CCWGG	1 cut(s)	983
Bst4CI	ACNGT	1 cut(s)	389
Bst6I	CTCTTC	1 cut(s)	921
BstACI	GRCGYC	1 cut(s)	5
BstC8I	GCNNGC	2 cut(s)	100, 253
BstDEI	CTNAG	5 cut(s)	492, 555, 696, 789, 900
BstDSI	CCRYGG	1 cut(s)	713
BstF5I	GGATG	3 cut(s)	217, 228, 977
BstFNI	CGCG	2 cut(s)	34, 128
BstKTI	GATC	4 cut(s)	132, 174, 183, 760
BstMAI	GTCTC	2 cut(s)	104, 1007
BstMBI	GATC	4 cut(s)	129, 171, 180, 757
BstMWI	GCNNNNNNNGC	4 cut(s)	40, 49, 928, 1095
BstNI	CCWGG	1 cut(s)	983
BstNSI	RCATGY	2 cut(s)	238, 531
BstPAI	GACNNNNGTC	1 cut(s)	35
BstSCI	CCNGG	1 cut(s)	981
BstSLI	GKGCMC	1 cut(s)	487
BstUI	CGCG	2 cut(s)	34, 128
BstV1I	GCAGC	2 cut(s)	376, 423
BstX2I	RGATCY	1 cut(s)	757
BstYI	RGATCY	1 cut(s)	757
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	2 cut(s)	177, 987
BtgI	CCRYGG	1 cut(s)	713
BtsCI	GGATG	3 cut(s)	217, 228, 977
BtuMI	TCGCGA	1 cut(s)	128
Cac8I	GCNNGC	2 cut(s)	100, 253
CaiI	CAGNNNCTG	1 cut(s)	988
Cfr13I	GGNCC	3 cut(s)	154, 415, 985
CseI	GACGC	1 cut(s)	40
Csp6I	GTAC	1 cut(s)	46
CviAII	CATG	6 cut(s)	235, 528, 667, 714, 769, 1070
CviQI	GTAC	1 cut(s)	46
DdeI	CTNAG	5 cut(s)	492, 555, 696, 789, 900
DpnI	GATC	4 cut(s)	131, 173, 182, 759
DpnII	GATC	4 cut(s)	129, 171, 180, 757
EaeI	YGGCCR	1 cut(s)	175
Eam1104I	CTCTTC	1 cut(s)	921
EarI	CTCTTC	1 cut(s)	921
Eco130I	CCWWGG	2 cut(s)	84, 713
Eco47I	GGWCC	2 cut(s)	154, 415
Eco81I	CCTNAGG	1 cut(s)	555
Eco88I	CYCGRG	2 cut(s)	65, 724
EcoO109I	RGGNCCY	3 cut(s)	154, 415, 985
EcoRII	CCWGG	1 cut(s)	981
EcoT14I	CCWWGG	2 cut(s)	84, 713
ErhI	CCWWGG	2 cut(s)	84, 713
FaeI	CATG	6 cut(s)	238, 531, 670, 717, 772, 1073
FalI	AAGNNNNNCTT	2 cut(s)	737, 769
FaqI	GGGAC	2 cut(s)	428, 466
FatI	CATG	6 cut(s)	234, 527, 666, 713, 768, 1069
FauI	CCCGC	2 cut(s)	27, 443
Fnu4HI	GCNGC	2 cut(s)	365, 437
FokI	GGATG	3 cut(s)	204, 215, 964
Fsp4HI	GCNGC	2 cut(s)	365, 437
FspBI	CTAG	3 cut(s)	252, 440, 834
GluI	GCNGC	2 cut(s)	365, 437
GsaI	CCCAGC	2 cut(s)	56, 639
HaeIII	GGCC	2 cut(s)	177, 987
HapII	CCGG	1 cut(s)	21
HgaI	GACGC	1 cut(s)	40
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	6 cut(s)	238, 531, 670, 717, 772, 1073
HinfI	GANTC	9 cut(s)	61, 454, 616, 656, 750, 872, 887, 896, 991
HpaII	CCGG	1 cut(s)	21
HphI	GGTGA	1 cut(s)	256
Hpy188I	TCNGA	4 cut(s)	60, 222, 469, 903
Hpy188III	TCNNGA	5 cut(s)	109, 127, 140, 745, 860
Hpy99I	CGWCG	2 cut(s)	10, 110
HpyAV	CCTTC	1 cut(s)	145
HpyCH4III	ACNGT	1 cut(s)	389
HpyCH4IV	ACGT	3 cut(s)	105, 546, 1045
HpyCH4V	TGCA	2 cut(s)	317, 731
HpyF10VI	GCNNNNNNNGC	4 cut(s)	40, 49, 928, 1095
HpyF3I	CTNAG	5 cut(s)	492, 555, 696, 789, 900
HpySE526I	ACGT	3 cut(s)	105, 546, 1045
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	6 cut(s)	238, 531, 670, 717, 772, 1073
Kzo9I	GATC	4 cut(s)	129, 171, 180, 757
LguI	GCTCTTC	1 cut(s)	921
LmnI	GCTCC	1 cut(s)	584
Lsp1109I	GCAGC	2 cut(s)	376, 423
MaeI	CTAG	3 cut(s)	252, 440, 834
MaeII	ACGT	3 cut(s)	105, 546, 1045
MaeIII	GTNAC	2 cut(s)	286, 946
MalI	GATC	4 cut(s)	131, 173, 182, 759
MbiI	CCGCTC	1 cut(s)	931
MboI	GATC	4 cut(s)	129, 171, 180, 757
MboII	GAAGA	5 cut(s)	339, 350, 518, 665, 938
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	1 cut(s)	757
MhlI	GDGCHC	1 cut(s)	487
MlyI	GAGTC	2 cut(s)	55, 448
MmeI	TCCRAC	2 cut(s)	38, 684
MseI	TTAA	8 cut(s)	209, 459, 629, 690, 813, 1028, 1091, 1105
MslI	CAYNNNNRTG	2 cut(s)	222, 233
MspI	CCGG	1 cut(s)	21
MspR9I	CCNGG	1 cut(s)	983
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	1 cut(s)	961
MvaI	CCWGG	1 cut(s)	983
MvnI	CGCG	2 cut(s)	34, 128
MwoI	GCNNNNNNNGC	4 cut(s)	40, 49, 928, 1095
NcoI	CCATGG	1 cut(s)	713
NdeII	GATC	4 cut(s)	129, 171, 180, 757
NheI	GCTAGC	1 cut(s)	251
NlaIII	CATG	6 cut(s)	238, 531, 670, 717, 772, 1073
NlaIV	GGNNCC	3 cut(s)	416, 759, 942
NmuCI	GTSAC	2 cut(s)	286, 946
NruI	TCGCGA	1 cut(s)	128
NspI	RCATGY	2 cut(s)	238, 531
PaeR7I	CTCGAG	1 cut(s)	724
PasI	CCCWGGG	1 cut(s)	982
PciI	ACATGT	1 cut(s)	527
PciSI	GCTCTTC	1 cut(s)	921
PcsI	WCGNNNNNNNCGW	2 cut(s)	45, 102
PctI	GAATGC	1 cut(s)	961
PfeI	GAWTC	7 cut(s)	616, 656, 750, 872, 887, 896, 991
PkrI	GCNGC	2 cut(s)	366, 438
PleI	GAGTC	2 cut(s)	55, 448
PpsI	GAGTC	2 cut(s)	55, 448
PpuMI	RGGWCCY	2 cut(s)	154, 415
PscI	ACATGT	1 cut(s)	527
PshAI	GACNNNNGTC	1 cut(s)	35
PshBI	ATTAAT	1 cut(s)	209
PsiI	TTATAA	1 cut(s)	371
Psp5II	RGGWCCY	2 cut(s)	154, 415
Psp6I	CCWGG	1 cut(s)	981
PspFI	CCCAGC	2 cut(s)	52, 635
PspGI	CCWGG	1 cut(s)	981
PspN4I	GGNNCC	3 cut(s)	416, 759, 942
PspPI	GGNCC	3 cut(s)	154, 415, 985
PspPPI	RGGWCCY	2 cut(s)	154, 415
PspXI	VCTCGAGB	1 cut(s)	724
PstNI	CAGNNNCTG	1 cut(s)	988
PsuI	RGATCY	1 cut(s)	757
RruI	TCGCGA	1 cut(s)	128
RsaI	GTAC	1 cut(s)	47
RsaNI	GTAC	1 cut(s)	46
RseI	CAYNNNNRTG	2 cut(s)	222, 233
SapI	GCTCTTC	1 cut(s)	921
SaqAI	TTAA	8 cut(s)	209, 459, 629, 690, 813, 1028, 1091, 1105
SatI	GCNGC	2 cut(s)	365, 437
Sau3AI	GATC	4 cut(s)	129, 171, 180, 757
Sau96I	GGNCC	3 cut(s)	154, 415, 985
SchI	GAGTC	2 cut(s)	55, 448
ScrFI	CCNGG	1 cut(s)	983
SduI	GDGCHC	1 cut(s)	487
Sfr274I	CTCGAG	1 cut(s)	724
SinI	GGWCC	2 cut(s)	154, 415
SlaI	CTCGAG	1 cut(s)	724
SmiMI	CAYNNNNRTG	2 cut(s)	222, 233
SmlI	CTYRAG	2 cut(s)	158, 724
SmoI	CTYRAG	2 cut(s)	158, 724
SsiI	CCGC	5 cut(s)	34, 43, 232, 450, 931
SspMI	CTAG	3 cut(s)	252, 440, 834
StyD4I	CCNGG	1 cut(s)	981
StyI	CCWWGG	2 cut(s)	84, 713
TaaI	ACNGT	1 cut(s)	389
TaiI	ACGT	3 cut(s)	108, 549, 1048
TaqI	TCGA	5 cut(s)	8, 14, 108, 141, 725
TfiI	GAWTC	7 cut(s)	616, 656, 750, 872, 887, 896, 991
Tru1I	TTAA	8 cut(s)	209, 459, 629, 690, 813, 1028, 1091, 1105
Tru9I	TTAA	8 cut(s)	209, 459, 629, 690, 813, 1028, 1091, 1105
TseFI	GTSAC	2 cut(s)	286, 946
TseI	GCWGC	2 cut(s)	364, 436
Tsp45I	GTSAC	2 cut(s)	286, 946
TspDTI	ATGAA	5 cut(s)	218, 357, 398, 900, 968
VpaK11BI	GGWCC	2 cut(s)	154, 415
VspI	ATTAAT	1 cut(s)	209
XapI	RAATTY	3 cut(s)	281, 430, 1030
XceI	RCATGY	2 cut(s)	238, 531
XhoI	CTCGAG	1 cut(s)	724
XspI	CTAG	3 cut(s)	252, 440, 834

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.