Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010177

Physical Location & Seq

Genomic Coordinates Forward (+)

41992768 .. 41996123

Gene Structure 3356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1287500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2397 bp

ATGGCTACCAATATTGTTGTTTTTCTTGTTACTTTCTCCATTGCTTGCTTTGCCATTTCTTCCAATGCCGGAAGAGTCCTAGACTCGAACAATGTTGCAAGCAACAATGGTGGTAGCAACTTCACTCATGTATGTGATCCTTCAAGGTATTCTGACCTTGGTTTGGACATGTCAGCCTTTGCCTTTTGCGACTCTAAACTTTCGTATGGCGATAGGGCCAAGGACTTGGTAGATAGGATGACACTGGCGGAGAAAGTGAAGCAGCTTGGAAATAATGCAAAGGGGGTTCCAAGGTTGGGGTTGCCAAAGTATGAGTGGTGGTCTGAGGCACTCCACGGTGTGTCAAATGTTGGTCCCGGGACTTGTTTCGATGATCTTATTCCGGGTGCAACAAGCTTCCCCACTGTTATTCTTACCACCGCAGCGTTCAACCGGTCTTTGTGGAAGCAAATTGGTCAGGTTGTTTCAACAGAAGCAAGAGCCATGTACAATCTAGGAAGAGCCGGGTTGACATATTGGAGTCCCAACGTTAATGTAGTGAGGGATCCGAGGTGGGGAAGAGCCACGGAAACACCCGGTGAAGACCCTTATGTTGTTGGCGTTTATGCCTCTAACTACGTCAGAGGCTTGCAGGATGTTGAGGGCACAGAGAATGCTACAGATTTGAACTCTAGACCTCTCAAAGTTTCCTCATGCTGCAAACATTATGCCGCCTACGATGTAGATAACTGGCTCGGTGTTGATCGTTACCATTTTGATGCCAGGGTGACGGAGCAAGATATGGCGGAAACTTTCCTAAAACCATTCGAGATGTGTATTAAGGATGGTGATGTAAGCAGTGTAATGTGCTCTTATAACCGTGTTAATGGCATACCTACTTGTGCTGATCCGAAACTCTTGACAGATACCATTAGAGGAGAGTGGGATCTTCACGGATATATAGTCGCGGATTGTGACTCTCTTGAAGTTATGGTGAACCACCACAAATGGCTCAACGACACCAATGAGGATGCTGCTGCACAAGCTTTGAAAGCAGGGTTGGATTTGGACTGTGGAGACTACTACCCAAACTTCACTCAAAGTTCGGTGAGGCAAGGGAAGGTGAAGGAGGTAGAAATCGACCGATCACTCAAGAACCTTTATGTGGTGCTTATGAGGCTAGGTTTCTTCGATGGGAATCCCACTTTTAATTCTCTTGGCAAGAAGGATGTGTGCTCCAAACAACATATTGAATTAGCAACAGAAGCAGCAAGGGAAGGAATCGTTTTATTGAAAAATGTCAATGAAACCTTGCCGTTGAAATCGAAGAAAATAAAAAAACTAGCCATCGTCGGGCCTCATGCCAATGCTACTGCAGCAATGATTGGAAACTATGCAGGTATTCCTTGCCAATTCACTTCACCTCTCGATGCATTTTCATCCTATGGAGAAGTTAAGTATGAAATGGGATGTGATGGAGTTGCATGTGCAAATGATAGATTCCCTGCCATGAGAGCCGCAAAGCACGCAGATGCAACCATAATTTTTGCTGGGTTGGACTTATCAGTTGAGGCAGAGAGATTGGATAGGGTAGATCTGCTTCTTCCTGGCTACCAAACCCAGTTTATCAATCAAGTTGCTCAAGTCTCCAAGGGTCCAGTGACTCTTGTAATCATGTCAGCTGGTGGTGTTGATATCAGTTTTGCCAAACAAAATGATAACATCAATGCAATATTGTGGGCTGGGTACCCGGGGGAGGAAGGCGGTCGTGCCATTGCAGATGTTGCTTTCGGACATTACAATCCTGGAGGAAGATTGCCTCTTACATGGTATGAAGCTGGTTACGTGGATATGCTACCCATGACATCCATGCAATTGAGACCAATTGATAGCCTAGGCTACCCCGGTCGTACATACAAATTCTACAATGGCTCCACAGTTTACCCATTTGGATACGGCCTTAGCTACACCCAGTTCAACTACACTCTGCGATATGCAAAGAGATCTCTGGATATAAACTTAAGAAAGTCCCAACACTGCCGCGACATAGAGTATAAGGATCATGAATACAAGCCACCTTGCCCTGCAGTCCTTATTGACGACTTGCAATGCAACCATGAATTTGGGTTCGGGGTTGAAGTTAAAAATGTGGGTAAGAGGGATGGAAGTGAAGTCATCATCGTTTACTCAAAACCACCAAATGGCATCGCCGCTACGCATGCCAAGCAAGTGATTGGGTTTAAAAGGGTTTTTGTGAAAGCCGGAAAAAGCGAAACTGTTAACTTTGTGTTCAATGCCTGCAAGAGCTTGGGACTTGTCAACTACAATGCTTACAATCTTCTAACATCAGGCAGACACACAATTTCGATTGGCGATGATGTCATCTCCTTCCCTATTCAAGTCAACATTAAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

799

Amino Acids

87.87

Weight (kDa)

6.1

Isoelectric Point (pI)

29.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	129 - 379	6.1e-35	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	421 - 649	2.5e-47	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	717 - 786	6.1e-09	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09700
fragaria_vesca	FvH4_3g05990
malus_domestica	MD05G1287500.v1.1 MD05G1287700.v1.1 MD05G1288600.v1.1 MD10G1264800.v1.1 MD17G1089800.v1.1
prunus_persica	Prupe.4G077200_v2.0.a1
pyrus_communis	pycom05g26600
rosa_chinensis	RchiOBHm_Chr5g0013421
rosa_laevigata	RLG00000032018
rosa_multiflora	Rmu_sc0003458.1_g000016 Rmu_sc0003458.1_g000017
rosa_roxburghii	Rroxscaffold_1G00063140
rosa_rugosa	Rorug05G0009200 Rorug05G0009300
rosa_samantha	Rh5AG104600 Rh5BG100900 Rh5CG112800 Rh5DG099500
rosa_wichuraiana	Rw5G009120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	855
Acc36I	ACCTGC	1 cut(s)	1367
Acc65I	GGTACC	1 cut(s)	1725
AccB1I	GGYRCC	1 cut(s)	1725
AccB7I	CCANNNNNTGG	1 cut(s)	2180
AccII	CGCG	2 cut(s)	947, 2022
AciI	CCGC	9 cut(s)	248, 420, 711, 785, 947, 1497, 1743, 2020, 2190
AclI	AACGTT	1 cut(s)	528
AclWI	GGATC	6 cut(s)	131, 539, 552, 881, 933, 2046
AcsI	RAATTY	2 cut(s)	1898, 2099
AdeI	CACNNNGTG	2 cut(s)	340, 578
AfaI	GTAC	3 cut(s)	488, 1727, 1891
AfiI	CCNNNNNNNGG	7 cut(s)	163, 296, 554, 1144, 1332, 1735, 2180
AflII	CTTAAG	1 cut(s)	1999
AflIII	ACRYGT	1 cut(s)	168
AgeI	ACCGGT	1 cut(s)	432
AjnI	CCWGG	3 cut(s)	761, 1585, 1783
AjuI	GAANNNNNNNTTGG	2 cut(s)	1022, 1054
AluBI	AGCT	7 cut(s)	265, 396, 1025, 1661, 1817, 1944, 2286
AluI	AGCT	7 cut(s)	265, 396, 1025, 1661, 1817, 1944, 2286
Alw21I	GWGCWC	2 cut(s)	851, 1217
Alw26I	GTCTC	3 cut(s)	1050, 1630, 1852
AlwI	GGATC	6 cut(s)	131, 539, 552, 881, 933, 2046
Ama87I	CYCGRG	2 cut(s)	356, 1729
AoxI	GGCC	3 cut(s)	216, 1334, 1936
ApeKI	GCWGC	7 cut(s)	262, 422, 696, 1013, 1016, 1247, 1355
ApoI	RAATTY	2 cut(s)	1898, 2099
AsiGI	ACCGGT	1 cut(s)	432
Asp718I	GGTACC	1 cut(s)	1725
AspA2I	CCTAGG	1 cut(s)	1873
AspS9I	GGNCC	4 cut(s)	216, 353, 1334, 1634
AsuC2I	CCSGG	8 cut(s)	357, 358, 384, 505, 576, 1730, 1731, 1884
AsuHPI	GGTGA	7 cut(s)	590, 778, 839, 985, 1099, 1114, 1392
AvaI	CYCGRG	2 cut(s)	356, 1729
AvaII	GGWCC	2 cut(s)	353, 1634
AvrII	CCTAGG	1 cut(s)	1873
BaeGI	GKGCMC	1 cut(s)	647
BamHI	GGATCC	1 cut(s)	544
BanI	GGYRCC	1 cut(s)	1725
BarI	GAAGNNNNNNTAC	2 cut(s)	1563, 1595
BbsI	GAAGAC	1 cut(s)	588
Bbv12I	GWGCWC	2 cut(s)	851, 1217
BbvI	GCAGC	7 cut(s)	274, 434, 683, 1000, 1003, 1259, 1367
BccI	CCATC	5 cut(s)	818, 1166, 1334, 1448, 2135
BceAI	ACGGC	2 cut(s)	1279, 1951
BciT130I	CCWGG	3 cut(s)	763, 1587, 1785
BciVI	GTATCC	1 cut(s)	1925
BcnI	CCSGG	8 cut(s)	357, 358, 384, 505, 576, 1730, 1731, 1884
BcoDI	GTCTC	3 cut(s)	1050, 1630, 1852
BfaI	CTAG	6 cut(s)	80, 494, 672, 1160, 1322, 1874
BfmI	CTRYAG	3 cut(s)	657, 1353, 2064
BfrI	CTTAAG	1 cut(s)	1999
BfuAI	ACCTGC	1 cut(s)	1367
BfuI	GTATCC	1 cut(s)	1925
BglII	AGATCT	2 cut(s)	1573, 1982
BlnI	CCTAGG	1 cut(s)	1873
Bme18I	GGWCC	2 cut(s)	353, 1634
BmeT110I	CYCGRG	2 cut(s)	356, 1729
BmgT120I	GGNCC	4 cut(s)	216, 353, 1334, 1634
BmiI	GGNNCC	6 cut(s)	288, 355, 546, 1635, 1727, 1912
BmrI	ACTGGG	2 cut(s)	1594, 1945
BmsI	GCATC	5 cut(s)	748, 1000, 1399, 1501, 2193
BmuI	ACTGGG	2 cut(s)	1594, 1945
BpiI	GAAGAC	1 cut(s)	588
BpmI	CTGGAG	1 cut(s)	1806
Bpu10I	CCTNAGC	1 cut(s)	1940
BpuEI	CTTGAG	2 cut(s)	1115, 1605
BpuMI	CCSGG	8 cut(s)	357, 358, 384, 505, 576, 1730, 1731, 1884
BsaAI	YACGTR	1 cut(s)	1825
BsaBI	GATNNNNATC	1 cut(s)	1176
BsaI	GGTCTC	1 cut(s)	1852
BsaWI	WCCGGW	1 cut(s)	432
BsaXI	ACNNNNNCTCC	2 cut(s)	1895, 1925
Bsc4I	CCNNNNNNNGG	7 cut(s)	163, 296, 554, 1144, 1332, 1735, 2180
Bse118I	RCCGGY	1 cut(s)	432
Bse1I	ACTGG	5 cut(s)	249, 734, 1600, 1637, 1951
Bse3DI	GCAATG	4 cut(s)	39, 1365, 1752, 2093
Bse8I	GATNNNNATC	1 cut(s)	1176
BseBI	CCWGG	3 cut(s)	763, 1587, 1785
BseGI	GGATG	9 cut(s)	243, 640, 829, 1015, 1213, 1418, 1454, 1844, 2146
BseJI	GATNNNNATC	1 cut(s)	1176
BseLI	CCNNNNNNNGG	7 cut(s)	163, 296, 554, 1144, 1332, 1735, 2180
BseMI	GCAATG	4 cut(s)	39, 1365, 1752, 2093
BseMII	CTCAG	1 cut(s)	315
BseNI	ACTGG	5 cut(s)	249, 734, 1600, 1637, 1951
BseRI	GAGGAG	1 cut(s)	930
BseSI	GKGCMC	1 cut(s)	647
BseXI	GCAGC	7 cut(s)	274, 434, 683, 1000, 1003, 1259, 1367
BseYI	CCCAGC	2 cut(s)	1529, 1721
BsgI	GTGCAG	1 cut(s)	1002
Bsh1236I	CGCG	2 cut(s)	947, 2022
Bsh1285I	CGRYCG	3 cut(s)	1123, 1747, 1888
BshFI	GGCC	3 cut(s)	218, 1336, 1938
BshNI	GGYRCC	1 cut(s)	1725
BshTI	ACCGGT	1 cut(s)	432
BsiEI	CGRYCG	3 cut(s)	1123, 1747, 1888
BsiHKAI	GWGCWC	2 cut(s)	851, 1217
BsiHKCI	CYCGRG	2 cut(s)	356, 1729
BsiSI	CCGG	9 cut(s)	69, 357, 383, 433, 504, 576, 1730, 1884, 2241
BslFI	GGGAC	5 cut(s)	339, 373, 507, 1993, 2304
BslI	CCNNNNNNNGG	7 cut(s)	163, 296, 554, 1144, 1332, 1735, 2180
BsmAI	GTCTC	3 cut(s)	1050, 1630, 1852
BsmFI	GGGAC	5 cut(s)	339, 373, 507, 1993, 2304
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	3 cut(s)	218, 1336, 1938
Bso31I	GGTCTC	1 cut(s)	1852
BsoBI	CYCGRG	2 cut(s)	356, 1729
Bsp1286I	GDGCHC	3 cut(s)	647, 851, 1217
Bsp1407I	TGTACA	1 cut(s)	486
BspACI	CCGC	9 cut(s)	248, 420, 711, 785, 947, 1497, 1743, 2020, 2190
BspANI	GGCC	3 cut(s)	218, 1336, 1938
BspCNI	CTCAG	1 cut(s)	316
BspFNI	CGCG	2 cut(s)	947, 2022
BspHI	TCATGA	1 cut(s)	2041
BspLI	GGNNCC	6 cut(s)	288, 355, 546, 1635, 1727, 1912
BspMAI	CTGCAG	2 cut(s)	1357, 2068
BspMI	ACCTGC	1 cut(s)	1367
BspPI	GGATC	6 cut(s)	131, 539, 552, 881, 933, 2046
BspQI	GCTCTTC	2 cut(s)	493, 553
BspT107I	GGYRCC	1 cut(s)	1725
BspTI	CTTAAG	1 cut(s)	1999
BspTNI	GGTCTC	1 cut(s)	1852
BsrDI	GCAATG	4 cut(s)	39, 1365, 1752, 2093
BsrFI	RCCGGY	1 cut(s)	432
BsrGI	TGTACA	1 cut(s)	486
BsrI	ACTGG	5 cut(s)	249, 734, 1600, 1637, 1951
BssAI	RCCGGY	1 cut(s)	432
BssT1I	CCWWGG	5 cut(s)	157, 219, 290, 1629, 1873
Bst2UI	CCWGG	3 cut(s)	763, 1587, 1785
Bst4CI	ACNGT	6 cut(s)	338, 406, 860, 1052, 1918, 2257
Bst6I	CTCTTC	3 cut(s)	67, 493, 553
BstAFI	CTTAAG	1 cut(s)	1999
BstAPI	GCANNNNNTGC	1 cut(s)	1763
BstAUI	TGTACA	1 cut(s)	486
BstBAI	YACGTR	1 cut(s)	1825
BstC8I	GCNNGC	6 cut(s)	46, 100, 629, 1506, 2199, 2278
BstDEI	CTNAG	2 cut(s)	324, 1940
BstDSI	CCRYGG	2 cut(s)	334, 564
BstF5I	GGATG	9 cut(s)	243, 640, 829, 1015, 1213, 1418, 1454, 1844, 2146
BstFNI	CGCG	2 cut(s)	947, 2022
BstMAI	GTCTC	3 cut(s)	1050, 1630, 1852
BstMCI	CGRYCG	3 cut(s)	1123, 1747, 1888
BstNI	CCWGG	3 cut(s)	763, 1587, 1785
BstNSI	RCATGY	3 cut(s)	172, 1467, 2201
BstSFI	CTRYAG	3 cut(s)	657, 1353, 2064
BstSLI	GKGCMC	1 cut(s)	647
BstUI	CGCG	2 cut(s)	947, 2022
BstV1I	GCAGC	7 cut(s)	274, 434, 683, 1000, 1003, 1259, 1367
BstV2I	GAAGAC	1 cut(s)	588
BstX2I	RGATCY	4 cut(s)	544, 925, 1573, 1982
BstXI	CCANNNNNNTGG	2 cut(s)	226, 2102
BstYI	RGATCY	4 cut(s)	544, 925, 1573, 1982
BsuI	GTATCC	1 cut(s)	1925
BsuRI	GGCC	3 cut(s)	218, 1336, 1938
BtgI	CCRYGG	2 cut(s)	334, 564
BtgZI	GCGATG	2 cut(s)	2170, 2367
BtsCI	GGATG	9 cut(s)	243, 640, 829, 1015, 1213, 1418, 1454, 1844, 2146
BtsI	GCAGTG	2 cut(s)	844, 2014
BtsIMutI	CAGTG	5 cut(s)	242, 402, 844, 1644, 2014
BveI	ACCTGC	1 cut(s)	1367
Cac8I	GCNNGC	6 cut(s)	46, 100, 629, 1506, 2199, 2278
CciI	TCATGA	1 cut(s)	2041
Cfr10I	RCCGGY	1 cut(s)	432
Cfr13I	GGNCC	4 cut(s)	216, 353, 1334, 1634
Cfr9I	CCCGGG	2 cut(s)	356, 1729
Csp6I	GTAC	3 cut(s)	487, 1726, 1890
CspAI	ACCGGT	1 cut(s)	432
CspCI	CAANNNNNGTGG	2 cut(s)	91, 126
CviQI	GTAC	3 cut(s)	487, 1726, 1890
DdeI	CTNAG	2 cut(s)	324, 1940
DraI	TTTAAA	1 cut(s)	2221
DraIII	CACNNNGTG	2 cut(s)	340, 578
Eam1104I	CTCTTC	3 cut(s)	67, 493, 553
EarI	CTCTTC	3 cut(s)	67, 493, 553
EciI	GGCGGA	2 cut(s)	263, 800
Eco130I	CCWWGG	5 cut(s)	157, 219, 290, 1629, 1873
Eco31I	GGTCTC	1 cut(s)	1852
Eco32I	GATATC	1 cut(s)	1675
Eco47I	GGWCC	2 cut(s)	353, 1634
Eco88I	CYCGRG	2 cut(s)	356, 1729
EcoRII	CCWGG	3 cut(s)	761, 1585, 1783
EcoRV	GATATC	1 cut(s)	1675
EcoT14I	CCWWGG	5 cut(s)	157, 219, 290, 1629, 1873
EcoT22I	ATGCAT	1 cut(s)	1414
ErhI	CCWWGG	5 cut(s)	157, 219, 290, 1629, 1873
FaqI	GGGAC	5 cut(s)	339, 373, 507, 1993, 2304
FokI	GGATG	9 cut(s)	250, 647, 836, 1022, 1220, 1405, 1461, 1831, 2153
FspBI	CTAG	6 cut(s)	80, 494, 672, 1160, 1322, 1874
GsaI	CCCAGC	2 cut(s)	1533, 1725
GsuI	CTGGAG	1 cut(s)	1806
HaeIII	GGCC	3 cut(s)	218, 1336, 1938
HapII	CCGG	9 cut(s)	69, 357, 383, 433, 504, 576, 1730, 1884, 2241
HincII	GTYRAC	4 cut(s)	510, 2260, 2299, 2383
HindII	GTYRAC	4 cut(s)	510, 2260, 2299, 2383
HindIII	AAGCTT	2 cut(s)	394, 1023
HinfI	GANTC	9 cut(s)	75, 83, 191, 520, 956, 1177, 1260, 1479, 1642
HpaI	GTTAAC	1 cut(s)	2260
HpaII	CCGG	9 cut(s)	69, 357, 383, 433, 504, 576, 1730, 1884, 2241
HphI	GGTGA	7 cut(s)	590, 778, 839, 985, 1099, 1114, 1392
Hpy166II	GTNNAC	7 cut(s)	510, 976, 1921, 2164, 2260, 2299, 2383
Hpy188I	TCNGA	6 cut(s)	154, 325, 549, 623, 891, 1772
Hpy188III	TCNNGA	8 cut(s)	672, 808, 898, 962, 1132, 1406, 1988, 2042
Hpy8I	GTNNAC	7 cut(s)	510, 976, 1921, 2164, 2260, 2299, 2383
Hpy99I	CGWCG	1 cut(s)	1334
HpyAV	CCTTC	7 cut(s)	150, 1093, 1099, 1198, 1250, 1733, 2377
HpyCH4III	ACNGT	6 cut(s)	338, 406, 860, 1052, 1918, 2257
HpyCH4IV	ACGT	3 cut(s)	528, 618, 1824
HpyF3I	CTNAG	2 cut(s)	324, 1940
HpySE526I	ACGT	3 cut(s)	528, 618, 1824
KpnI	GGTACC	1 cut(s)	1729
KspAI	GTTAAC	1 cut(s)	2260
LguI	GCTCTTC	2 cut(s)	493, 553
LmnI	GCTCC	3 cut(s)	772, 1220, 1916
Lsp1109I	GCAGC	7 cut(s)	274, 434, 683, 1000, 1003, 1259, 1367
LweI	GCATC	5 cut(s)	748, 1000, 1399, 1501, 2193
MaeI	CTAG	6 cut(s)	80, 494, 672, 1160, 1322, 1874
MaeII	ACGT	3 cut(s)	528, 618, 1824
MaeIII	GTNAC	6 cut(s)	28, 746, 766, 953, 1639, 1820
MfeI	CAATTG	2 cut(s)	1853, 1863
MflI	RGATCY	4 cut(s)	544, 925, 1573, 1982
MhlI	GDGCHC	3 cut(s)	647, 851, 1217
MlyI	GAGTC	6 cut(s)	77, 84, 185, 529, 950, 1636
MmeI	TCCRAC	2 cut(s)	1020, 1515
Mph1103I	ATGCAT	1 cut(s)	1414
MslI	CAYNNNNRTG	4 cut(s)	132, 756, 1344, 1509
MspA1I	CMGCKG	1 cut(s)	1661
MspCI	CTTAAG	1 cut(s)	1999
MspI	CCGG	9 cut(s)	69, 357, 383, 433, 504, 576, 1730, 1884, 2241
MunI	CAATTG	2 cut(s)	1853, 1863
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	3 cut(s)	763, 1587, 1785
MvnI	CGCG	2 cut(s)	947, 2022
NciI	CCSGG	8 cut(s)	357, 358, 384, 505, 576, 1730, 1731, 1884
NlaIV	GGNNCC	6 cut(s)	288, 355, 546, 1635, 1727, 1912
NmuCI	GTSAC	3 cut(s)	766, 953, 1639
NsiI	ATGCAT	1 cut(s)	1414
NspI	RCATGY	3 cut(s)	172, 1467, 2201
PaeI	GCATGC	1 cut(s)	2201
PagI	TCATGA	1 cut(s)	2041
PciI	ACATGT	1 cut(s)	168
PciSI	GCTCTTC	2 cut(s)	493, 553
PctI	GAATGC	1 cut(s)	658
PfeI	GAWTC	3 cut(s)	1177, 1260, 1479
PflMI	CCANNNNNTGG	1 cut(s)	2180
PfoI	TCCNGGA	1 cut(s)	1783
PinAI	ACCGGT	1 cut(s)	432
PleI	GAGTC	6 cut(s)	77, 83, 185, 528, 950, 1636
PpsI	GAGTC	6 cut(s)	77, 83, 185, 528, 950, 1636
Ppu21I	YACGTR	1 cut(s)	1825
PscI	ACATGT	1 cut(s)	168
PsiI	TTATAA	1 cut(s)	855
Psp1406I	AACGTT	1 cut(s)	528
Psp6I	CCWGG	3 cut(s)	761, 1585, 1783
PspFI	CCCAGC	2 cut(s)	1529, 1721
PspGI	CCWGG	3 cut(s)	761, 1585, 1783
PspN4I	GGNNCC	6 cut(s)	288, 355, 546, 1635, 1727, 1912
PspPI	GGNCC	4 cut(s)	216, 353, 1334, 1634
PstI	CTGCAG	2 cut(s)	1357, 2068
PsuI	RGATCY	4 cut(s)	544, 925, 1573, 1982
PvuII	CAGCTG	1 cut(s)	1661
RsaI	GTAC	3 cut(s)	488, 1727, 1891
RsaNI	GTAC	3 cut(s)	487, 1726, 1890
RseI	CAYNNNNRTG	4 cut(s)	132, 756, 1344, 1509
SapI	GCTCTTC	2 cut(s)	493, 553
Sau96I	GGNCC	4 cut(s)	216, 353, 1334, 1634
SchI	GAGTC	6 cut(s)	77, 84, 185, 529, 950, 1636
SduI	GDGCHC	3 cut(s)	647, 851, 1217
SfaNI	GCATC	5 cut(s)	748, 1000, 1399, 1501, 2193
SfcI	CTRYAG	3 cut(s)	657, 1353, 2064
SinI	GGWCC	2 cut(s)	353, 1634
SmaI	CCCGGG	2 cut(s)	358, 1731
SmiMI	CAYNNNNRTG	4 cut(s)	132, 756, 1344, 1509
SmlI	CTYRAG	3 cut(s)	1130, 1620, 1999
SmoI	CTYRAG	3 cut(s)	1130, 1620, 1999
SphI	GCATGC	1 cut(s)	2201
SsiI	CCGC	9 cut(s)	248, 420, 711, 785, 947, 1497, 1743, 2020, 2190
SspI	AATATT	2 cut(s)	13, 1713
SspMI	CTAG	6 cut(s)	80, 494, 672, 1160, 1322, 1874
StyI	CCWWGG	5 cut(s)	157, 219, 290, 1629, 1873
TaaI	ACNGT	6 cut(s)	338, 406, 860, 1052, 1918, 2257
TaiI	ACGT	3 cut(s)	531, 621, 1827
TaqI	TCGA	8 cut(s)	86, 369, 807, 1119, 1170, 1304, 1407, 2345
TaqII	GACCGA	1 cut(s)	1137
TatI	WGTACW	1 cut(s)	486
TauI	GCSGC	4 cut(s)	713, 1499, 2022, 2192
TfiI	GAWTC	3 cut(s)	1177, 1260, 1479
TscAI	CASTG	5 cut(s)	249, 409, 844, 1644, 2021
TseFI	GTSAC	3 cut(s)	766, 953, 1639
TseI	GCWGC	7 cut(s)	262, 422, 696, 1013, 1016, 1247, 1355
Tsp45I	GTSAC	3 cut(s)	766, 953, 1639
TspDTI	ATGAA	6 cut(s)	1299, 1407, 1455, 1827, 2058, 2112
TspGWI	ACGGA	3 cut(s)	581, 785, 948
TspMI	CCCGGG	2 cut(s)	356, 1729
TspRI	CASTG	5 cut(s)	249, 409, 844, 1644, 2021
Van91I	CCANNNNNTGG	1 cut(s)	2180
Vha464I	CTTAAG	1 cut(s)	1999
VpaK11BI	GGWCC	2 cut(s)	353, 1634
XapI	RAATTY	2 cut(s)	1898, 2099
XbaI	TCTAGA	1 cut(s)	671
XceI	RCATGY	3 cut(s)	172, 1467, 2201
XcmI	CCANNNNNNNNNTGG	1 cut(s)	312
XmaI	CCCGGG	2 cut(s)	356, 1729
XmaJI	CCTAGG	1 cut(s)	1873
XspI	CTAG	6 cut(s)	80, 494, 672, 1160, 1322, 1874
Zsp2I	ATGCAT	1 cut(s)	1414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.