MD05G1288600.v1.1

beta-xylosidase alpha-L-arabinofuranosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010177
Physical Location & Seq
Forward (+)
42117561 .. 42127216
9656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288600.v1.1.491

Sequence Viewer

Length: 4356 bp
ATGAGTGGTGGTCTGAGGCACTCCACGGTGTGTCAAATGTTGGTCCCGGGACTTATTTCGATGATCTTATTCCGGGTGCAACAAGCTTCCCCACTGTTACTCTTACCACCGCAGCGTTCAACCAGTCTTTGTGGAAGCAAATTGGTTGTTTCAACAGAAGCAAGAGCCATGTACAATCTAGGAAGAGCCGGGTTGACATATTGGAGTCCCAACGTTAATGTAGTGAGGGATCCGAGGTGGGGAAGAGCCACGGAAACACCCGGTGAAGACCCTTATGTTGTTGGCGTTTATGCCTCTAACTACGTCAGAGGCTTGCAGGATGTTGAGGGCACAGAGAATGCTACAGATTTGAACTCTAGACCTCTCGAAGTTTCCTCATGCTGCAAACATTATGCCGCCTACGATGTAGACAACTGGCTCGGTGTTGATCATTACCATTTTGATGCCAGGGTGACGGAGCAAGATATGGCAGAAACTTTCCTAAAACCATTCGAGATGTGTATTAAGGATGGCGATGTAAGCAGTGTAATGTGCTCTTATAACCGTGTTAATGGCATACCTACTTGTGCTGATCCGAAACTCCTGACACATACCATTAGAGGAGAGTGGGATCTTCACGGATATATAGTCGCGGATTGTGACTCTCTTGAAGTTATGGTGGACCACCACAAATGGCTCAGCGACACCAATGAGGATGCTGCTGCACAAGCTTTGAAAGCAGGCCATTTGTTGCACATGTGGTTCCGTTGGATTTTGGATTCTGGTGCAACAGATCATATGACGTATAATAAAACCTTGTTTCAATCTATGACACATCCTCATAGAAAGTGTGTGGCAACCGCCAATGGTTCTACTGCTGCTGTTGTTGGAGCGGGAATAGTGTCTCTTACACCCTCTCTTCCCTTACATAATTGTTTATTGGTTCTGTCATTGTCCCATCACTTATTGTCGGTACCTCAAGTCAATGAACAATTGGATTGTGTTGTATTAATGTATCCTTGTTTCAGGATATTTAGACAAAGGAAATCATTGGGCATGGCACTAAGAGAGAGGGTCTATATTATGTGGATGATGTGGTTCCTAGAAGAGCTAATTTGGTTCATGCGTCACATAATAGTAGTCTACAGAAGTGGTATTGAAGACTCAGAATTGAAATGTGAAGTATGTATTTTAGCCAAAAGTCATCGTGCTTCATTCCCTCCAAGTATGAATAGAACATCTTCTCCATTTGCGCTAGTACACTCTGATGTGTGGGGGCCTTCCCTTATTAGTACTACTTCGGGAATTAAATGGTTTATTACATTTGTTGATGATTGCACTTGTATGACGTGGGTTTATCCGTTGAAACATAAAAGTGATGTTATTTCAGGGGGAGTGCATGGTGCCGTGCCGAGTGATGCAGTTGATGCTCGGTCTCCTCCTATCGAAGAGCCTATTGTGAGTGTTCAGCAGCTTCTTACCGAAGAAGAGGCTGCCAGTCCGTGTGTTGGTGTTGCCGAAGAGGTTTGTACTGATGGTCAGTCAGCTGTTGCCGAAGCAGCTTCCTCTCCTCTCTCTAGCTCAACAGTGCCGTTGTCCAATACATCTTCTCTGGATATCCCTGAGGCAAGCACTAGTGATACTCATGTAACTAATGATGTTATAAGTACATATAAATTGCCACCAAGGCAAAATCGTGGTGTACCGCCTAACAGGTTCTCTCCGGAGGGAAAAGTGAAGTATCCCATAACCAATTATGTGTCCTGGTATAGCAACGGAGGAAAAATGGGAGTGTGCCAACTGCGAAAGTCCATTTATGGGCTTAAACAGTCACCATGTGCATGGTTTGGGAGGTTCACTCAGGCCATGAGAAAGAATGGGTATTTTCAGAGTCATTCTGATCATACTTTATTTGTGAAGCGGATGAACAATAAAGTAATAGCCTTGATTATTTATGTAGATGATATGATAATAACAAATGATGATTTAGATGAAATTACCAAGCTGGAGAAGAACCTTTCTGCCGAGTTTGAGATGAAAAATTTGGGTGACTTAAAGTATTTCCTTGGTGTTGAAGTTGCTCGTTTGTCCAGAGGTATTTTCTTGTCTCAACGAAAATATGTTCTAGATTTATTGAAGGAAACATGTATATTTGGGTGTGAACCAGTGGATACTCCTATTGTGGAGAAACATTACTTGGGAATTTATCCGGATCAAGAACCAATGGATAAAGGCAGATATCAGAGACTGGTAGGAAGACTAATTTATTTAGCTCATACTCATCCAGATATAGCCTATGCAGTAAGTTTGGTGAGTCAGTTTATGCACTCGCGGAGTGTGGATCATATGGCAGCTGTTATTCGAATTTTGGCTTATTTGAAGTTGGCTCCAGGAAAAGGAATTTTGTATAAAAATTCAGGTCATCTGAGAGTTGAGGGATTTACTGACGCTGATTGGGTAGGTGATGTGACTGATAGGCGCTCTACTTCTGGATACTTTACGTTTGTTGGTGGAAATCTAGTTACATGGAGAAGTAAGAAGCAAAATGTGGTGTCAAGGTCTTCAGCTGAGGCCGAATTTAGAGGTATGGCACATGGGATATGTGAAGTTCTTTGGTTACGTAAGTTACTTGGTGGTCTAGGTTTCAAAGAAAAGAAGGCAATAAGCTTGTATTGTGATAATAAGTCCGCACGGGAGATTGCAGAGAACCCGGTACAACATGATAGAACAAAGCATGTGGAGGTTGACCATCACTTTATTAATGAAAAGCTTGAGAAGAAAATTGTGTCAATACCATTTGTGAACTCAGAAGAGCAACTTGCAAATATCCTCACTCATGCCGTGTGTAGTAGGTTGTTTGGGATGGATTTGGACTGTGGAGACTACTACCCAAACTTCACTCAAAGTTCGGTGAGGCAAGGGAAGGTGAAGGAGGTAGAAATCGACCGATCACTCAAGAACCTTTATGTGGTGCTTATGAGGCTAGGTTTCTTCGATGGGAATCCCACTTTTAAGTCTCTTGGCAAGAAGGATGTGTGCTCCAAACAACATATTGAATTAGCAACAAAAGCAGCAAGGGAAGGAATCGTTTTATTGAAAAATGTCAATGAAACTTTGCCGTTGAAATCGAAGAAAATAAAAAAACTAGCCATCGTCGGGCCTCATGCCAATGCTACTGCAGCAATGATTGGAAACTATGCAGGTATTCCTTGCCAATTCACTTCACCTCTCGATGCATTTTCATCCTATGGAGAAGTTAAGTATGAAATGGGATGTGATGGAGTTGCATGTGCAAATGATAGTCTGATATTCCCTGCCATGAGGGCCGCGAAGCACGCAGATGCATCCATAATTTTTGCTGGGTCAGTTGAGGCAGAGAGCTTGGATAGGGTGGATCTGCTTCTTCCTGGCTACCAAACCCAGTTTATCAATCAAGTTGCTCAAGTCTCCAAGGGTCCAGTGATCCTTGTAATCATGTCAGCTGGTGGTGTTGATATCAGTTTTGCCAAACAAAATGATAACATCAATGCAATATTGTGGGCTGGGTACCCGGGGGAGGAAGGCGGTCGTGCCATTGCAGATGTTGCTTTCGGACATTACAATCCTGGAGGAAGATTGCCTCTTACATGGTATGAAGCTGGTTACGTGGATATGCTACCCATGACATCCATGCAATTGAGACCAATTGATAGCCTAGGCTACCCCGGTCGTACATACAAATTCTACAATGGCTCCACAGTTTACCCATTTGGATACGGCCTTAGCTACACCCAGTTCAACTACACTCTGCGATCTGCAAAGAGATCTCTGGATATAAACTTAAGAAAGTCCCAACACTGCCGCGACATAGAGTACAAGGATCATGAATACAAGCCACCTTGCCCTGTAGTCCTTATTGACGACTTGCAATGCAACCATGAATTGGGGTTCGGGGTTGAAGTTAAAAATGTGGGTAAGAGGGATGGAAGTGAAGTCATCATCGTTTACTCAAAACCACCAAATGGCATCGCCGCTACGCATGCCAAGCAAGTGATTGGGTTTAAAAGGGTTTTTGTGAAAGCCGGAAAAAGCGAAACTGTTAACTTTGTGTTCAATGCCTGCAAGAGCTTGGGACTTGTCAACTACAATGCTTACAATCTTCTAACATCAGGCAGACACACAATTTCGATTGGCGATGATGTCGTCTCCTTCCCTATTCAAGTTTTGCCTTGGAATCGAGTTGGATTAAGCACACCCGTTTTCCGGCGAACCCGTGAGCTTCAAGGGGATTTCCGGTCACCTCCAACCATGTCACGGTTATTTGAAGGTAAAGTTACTAGTGGAGAAATTCTTAATCCTTATCCGCGCGACTCTTCTGCTAAGGAATATTTGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

1452

Amino Acids

161.61

Weight (kDa)

7.67

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 66 - 247 3.4e-28 Glycosyl hydrolase family 3 N terminal domain
Pol_BBD PF22936 250 - 323 3e-13 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 587 - 721 1.6e-29 Reverse transcriptase (RNA-dependent DNA polymerase)
Glyco_hydro_3_C PF01915 1022 - 1249 5.1e-48 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 1317 - 1386 1.3e-08 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 540, 1643
Acc36I ACCTGC 1 cut(s) 3170
Acc65I GGTACC 2 cut(s) 952, 3525
AccB1I GGYRCC 3 cut(s) 952, 1382, 3525
AccB7I CCANNNNNTGG 2 cut(s) 3901, 3980
AccBSI CCGCTC 1 cut(s) 872
AccI GTMKAC 2 cut(s) 408, 1122
AccII CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
AccIII TCCGGA 2 cut(s) 1702, 2188
AclI AACGTT 1 cut(s) 213
AclWI GGATC 9 cut(s) 224, 237, 566, 618, 2199, 2328, 3381, 3436, 3846
AcsI RAATTY 8 cut(s) 2020, 2181, 2343, 2379, 2392, 2555, 3698, 4305
AcuI CTGAAG 1 cut(s) 2526
AdeI CACNNNGTG 3 cut(s) 30, 263, 1817
AflII CTTAAG 1 cut(s) 3799
AflIII ACRYGT 2 cut(s) 735, 2123
AhlI ACTAGT 2 cut(s) 1613, 4295
AjiI CACGTC 1 cut(s) 1329
AjnI CCWGG 5 cut(s) 446, 1742, 2368, 3385, 3583
AjuI GAANNNNNNNTTGG 2 cut(s) 2159, 2191
AloI GAACNNNNNNTCC 2 cut(s) 1207, 1239
Alw21I GWGCWC 2 cut(s) 536, 3020
Alw26I GTCTC 9 cut(s) 888, 1419, 2091, 2218, 2853, 3000, 3430, 3652, 4168
AlwI GGATC 9 cut(s) 224, 237, 566, 618, 2199, 2328, 3381, 3436, 3846
AlwNI CAGNNNCTG 1 cut(s) 2227
Ama87I CYCGRG 2 cut(s) 46, 3529
Aor13HI TCCGGA 2 cut(s) 1702, 2188
AoxI GGCC 7 cut(s) 721, 1256, 1842, 2550, 3137, 3303, 3736
ApoI RAATTY 8 cut(s) 2020, 2181, 2343, 2379, 2392, 2555, 3698, 4305
AseI ATTAAT 2 cut(s) 989, 2739
Asp700I GAANNNNTTC 1 cut(s) 1219
Asp718I GGTACC 2 cut(s) 952, 3525
AspA2I CCTAGG 1 cut(s) 3673
AspLEI GCGC 3 cut(s) 1234, 2460, 4326
AspS9I GGNCC 6 cut(s) 43, 661, 1256, 3137, 3303, 3434
AsuC2I CCSGG 9 cut(s) 47, 48, 74, 190, 261, 2690, 3530, 3531, 3684
AsuII TTCGAA 1 cut(s) 2341
AvaI CYCGRG 2 cut(s) 46, 3529
AvaII GGWCC 3 cut(s) 43, 661, 3434
AvrII CCTAGG 1 cut(s) 3673
AxyI CCTNAGG 1 cut(s) 1602
BaeGI GKGCMC 1 cut(s) 332
BaeI ACNNNNGTAYC 2 cut(s) 1611, 1644
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 952, 1382, 3525
BbsI GAAGAC 4 cut(s) 273, 1146, 2242, 2532
Bbv12I GWGCWC 2 cut(s) 536, 3020
BbvCI CCTCAGC 1 cut(s) 2547
BccI CCATC 9 cut(s) 503, 945, 1508, 2736, 2836, 2969, 3137, 3251, 3935
BceAI ACGGC 5 cut(s) 1370, 1555, 2804, 3082, 3751
BcgI CGANNNNNNTGC 2 cut(s) 4316, 4350
BciT130I CCWGG 5 cut(s) 448, 1744, 2370, 3387, 3585
BciVI GTATCC 5 cut(s) 1005, 1731, 2143, 2465, 3725
BclI TGATCA 2 cut(s) 427, 1879
BcnI CCSGG 9 cut(s) 47, 48, 74, 190, 261, 2690, 3530, 3531, 3684
BcoDI GTCTC 9 cut(s) 888, 1419, 2091, 2218, 2853, 3000, 3430, 3652, 4168
BcuI ACTAGT 2 cut(s) 1613, 4295
BfmI CTRYAG 4 cut(s) 342, 1123, 3156, 3864
BfoI RGCGCY 1 cut(s) 2461
BfrI CTTAAG 1 cut(s) 3799
BfuAI ACCTGC 1 cut(s) 3170
BfuI GTATCC 5 cut(s) 1005, 1731, 2143, 2465, 3725
BglI GCCNNNNNGGC 2 cut(s) 1666, 3302
BglII AGATCT 1 cut(s) 3782
BlnI CCTAGG 1 cut(s) 3673
BlpI GCTNAGC 1 cut(s) 677
BmcAI AGTACT 1 cut(s) 1273
Bme18I GGWCC 3 cut(s) 43, 661, 3434
BmeT110I CYCGRG 2 cut(s) 46, 3529
BmgBI CACGTC 1 cut(s) 1329
BmgT120I GGNCC 6 cut(s) 43, 661, 1256, 3137, 3303, 3434
BmrI ACTGGG 2 cut(s) 3394, 3745
BmsI GCATC 8 cut(s) 433, 685, 1387, 1396, 3202, 3310, 3332, 3993
BmuI ACTGGG 2 cut(s) 3394, 3745
BpiI GAAGAC 4 cut(s) 273, 1146, 2242, 2532
BplI GAGNNNNNCTC 2 cut(s) 1822, 1854
BpmI CTGGAG 3 cut(s) 2006, 2352, 3606
Bpu10I CCTNAGC 3 cut(s) 2547, 3740, 4338
Bpu1102I GCTNAGC 1 cut(s) 677
Bpu14I TTCGAA 1 cut(s) 2341
BpuEI CTTGAG 4 cut(s) 942, 2771, 2918, 3405
BpuMI CCSGG 9 cut(s) 47, 48, 74, 190, 261, 2690, 3530, 3531, 3684
BsaAI YACGTR 2 cut(s) 2600, 3625
BsaBI GATNNNNATC 1 cut(s) 2979
BsaI GGTCTC 2 cut(s) 1419, 3652
BsaWI WCCGGW 3 cut(s) 1702, 2188, 4251
BsaXI ACNNNNNCTCC 4 cut(s) 1207, 1237, 3695, 3725
Bse1I ACTGG 8 cut(s) 123, 419, 1476, 2144, 2232, 3400, 3437, 3751
Bse21I CCTNAGG 1 cut(s) 1602
Bse3DI GCAATG 3 cut(s) 3168, 3552, 3893
Bse8I GATNNNNATC 1 cut(s) 2979
BseAI TCCGGA 2 cut(s) 1702, 2188
BseBI CCWGG 5 cut(s) 448, 1744, 2370, 3387, 3585
BseJI GATNNNNATC 1 cut(s) 2979
BseMI GCAATG 3 cut(s) 3168, 3552, 3893
BseMII CTCAG 8 cut(s) 5, 691, 1158, 1593, 1853, 2396, 2538, 2799
BseNI ACTGG 8 cut(s) 123, 419, 1476, 2144, 2232, 3400, 3437, 3751
BseRI GAGGAG 3 cut(s) 615, 1407, 1539
BseSI GKGCMC 1 cut(s) 332
BseYI CCCAGC 2 cut(s) 3338, 3521
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
Bsh1285I CGRYCG 3 cut(s) 2926, 3547, 3688
BshFI GGCC 7 cut(s) 723, 1258, 1844, 2552, 3139, 3305, 3738
BshNI GGYRCC 3 cut(s) 952, 1382, 3525
BsiEI CGRYCG 3 cut(s) 2926, 3547, 3688
BsiHKAI GWGCWC 2 cut(s) 536, 3020
BsiHKCI CYCGRG 2 cut(s) 46, 3529
BslFI GGGAC 6 cut(s) 29, 63, 192, 919, 3793, 4104
BsmAI GTCTC 9 cut(s) 888, 1419, 2091, 2218, 2853, 3000, 3430, 3652, 4168
BsmBI CGTCTC 1 cut(s) 4168
BsmFI GGGAC 6 cut(s) 29, 63, 192, 919, 3793, 4104
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 7 cut(s) 723, 1258, 1844, 2552, 3139, 3305, 3738
Bso31I GGTCTC 2 cut(s) 1419, 3652
BsoBI CYCGRG 2 cut(s) 46, 3529
Bsp119I TTCGAA 1 cut(s) 2341
Bsp1286I GDGCHC 3 cut(s) 332, 536, 3020
Bsp13I TCCGGA 2 cut(s) 1702, 2188
Bsp1407I TGTACA 1 cut(s) 171
Bsp1720I GCTNAGC 1 cut(s) 677
BspANI GGCC 7 cut(s) 723, 1258, 1844, 2552, 3139, 3305, 3738
BspCNI CTCAG 8 cut(s) 6, 690, 1157, 1594, 1852, 2397, 2539, 2798
BspEI TCCGGA 2 cut(s) 1702, 2188
BspFNI CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
BspHI TCATGA 1 cut(s) 3841
BspMAI CTGCAG 1 cut(s) 3160
BspMI ACCTGC 1 cut(s) 3170
BspPI GGATC 9 cut(s) 224, 237, 566, 618, 2199, 2328, 3381, 3436, 3846
BspQI GCTCTTC 5 cut(s) 178, 238, 1080, 1422, 2784
BspT104I TTCGAA 1 cut(s) 2341
BspT107I GGYRCC 3 cut(s) 952, 1382, 3525
BspTI CTTAAG 1 cut(s) 3799
BspTNI GGTCTC 2 cut(s) 1419, 3652
BsrBI CCGCTC 1 cut(s) 872
BsrDI GCAATG 3 cut(s) 3168, 3552, 3893
BsrGI TGTACA 1 cut(s) 171
BsrI ACTGG 8 cut(s) 123, 419, 1476, 2144, 2232, 3400, 3437, 3751
BssT1I CCWWGG 5 cut(s) 1664, 2044, 3429, 3673, 4187
Bst2UI CCWGG 5 cut(s) 448, 1744, 2370, 3387, 3585
Bst4CI ACNGT 9 cut(s) 28, 96, 545, 1567, 1809, 2855, 3718, 4057, 4275
Bst6I CTCTTC 9 cut(s) 178, 238, 903, 1080, 1422, 1461, 1494, 2784, 4336
BstAFI CTTAAG 1 cut(s) 3799
BstAPI GCANNNNNTGC 2 cut(s) 1406, 3563
BstAUI TGTACA 1 cut(s) 171
BstBAI YACGTR 2 cut(s) 2600, 3625
BstBI TTCGAA 1 cut(s) 2341
BstC8I GCNNGC 6 cut(s) 314, 721, 1609, 3315, 3999, 4078
BstDSI CCRYGG 2 cut(s) 24, 249
BstEII GGTNACC 1 cut(s) 4254
BstFNI CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
BstH2I RGCGCY 1 cut(s) 2461
BstHHI GCGC 3 cut(s) 1234, 2460, 4326
BstMAI GTCTC 9 cut(s) 888, 1419, 2091, 2218, 2853, 3000, 3430, 3652, 4168
BstMCI CGRYCG 3 cut(s) 2926, 3547, 3688
BstNI CCWGG 5 cut(s) 448, 1744, 2370, 3387, 3585
BstNSI RCATGY 5 cut(s) 739, 2127, 2717, 3270, 4001
BstPI GGTNACC 1 cut(s) 4254
BstSFI CTRYAG 4 cut(s) 342, 1123, 3156, 3864
BstSLI GKGCMC 1 cut(s) 332
BstSNI TACGTA 1 cut(s) 2600
BstUI CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
BstV2I GAAGAC 4 cut(s) 273, 1146, 2242, 2532
BstX2I RGATCY 4 cut(s) 229, 610, 3373, 3782
BstXI CCANNNNNNTGG 1 cut(s) 1821
BstYI RGATCY 4 cut(s) 229, 610, 3373, 3782
Bsu36I CCTNAGG 1 cut(s) 1602
BsuI GTATCC 5 cut(s) 1005, 1731, 2143, 2465, 3725
BsuRI GGCC 7 cut(s) 723, 1258, 1844, 2552, 3139, 3305, 3738
BtgI CCRYGG 2 cut(s) 24, 249
BtgZI GCGATG 3 cut(s) 528, 3970, 4167
BtrI CACGTC 1 cut(s) 1329
BtsI GCAGTG 2 cut(s) 529, 3814
BtsIMutI CAGTG 6 cut(s) 92, 529, 1572, 2151, 3444, 3814
BveI ACCTGC 1 cut(s) 3170
Cac8I GCNNGC 6 cut(s) 314, 721, 1609, 3315, 3999, 4078
CaiI CAGNNNCTG 1 cut(s) 2227
CciI TCATGA 1 cut(s) 3841
CfoI GCGC 3 cut(s) 1234, 2460, 4326
Cfr13I GGNCC 6 cut(s) 43, 661, 1256, 3137, 3303, 3434
Cfr9I CCCGGG 2 cut(s) 46, 3529
CseI GACGC 2 cut(s) 1094, 2435
CspCI CAANNNNNGTGG 2 cut(s) 2697, 2732
DraI TTTAAA 1 cut(s) 4021
DraIII CACNNNGTG 3 cut(s) 30, 263, 1817
Eam1104I CTCTTC 9 cut(s) 178, 238, 903, 1080, 1422, 1461, 1494, 2784, 4336
EarI CTCTTC 9 cut(s) 178, 238, 903, 1080, 1422, 1461, 1494, 2784, 4336
Eco105I TACGTA 1 cut(s) 2600
Eco130I CCWWGG 5 cut(s) 1664, 2044, 3429, 3673, 4187
Eco31I GGTCTC 2 cut(s) 1419, 3652
Eco32I GATATC 3 cut(s) 1597, 2219, 3475
Eco47I GGWCC 3 cut(s) 43, 661, 3434
Eco57I CTGAAG 1 cut(s) 2526
Eco81I CCTNAGG 1 cut(s) 1602
Eco88I CYCGRG 2 cut(s) 46, 3529
Eco91I GGTNACC 1 cut(s) 4254
EcoO109I RGGNCCY 1 cut(s) 1256
EcoO65I GGTNACC 1 cut(s) 4254
EcoRII CCWGG 5 cut(s) 446, 1742, 2368, 3385, 3583
EcoRV GATATC 3 cut(s) 1597, 2219, 3475
EcoT14I CCWWGG 5 cut(s) 1664, 2044, 3429, 3673, 4187
EcoT22I ATGCAT 2 cut(s) 3217, 3325
ErhI CCWWGG 5 cut(s) 1664, 2044, 3429, 3673, 4187
Esp3I CGTCTC 1 cut(s) 4168
FalI AAGNNNNNCTT 2 cut(s) 2781, 2813
FaqI GGGAC 6 cut(s) 29, 63, 192, 919, 3793, 4104
FauI CCCGC 1 cut(s) 865
FauNDI CATATG 2 cut(s) 777, 2325
FbaI TGATCA 2 cut(s) 427, 1879
FblI GTMKAC 2 cut(s) 408, 1122
GlaI GCGC 3 cut(s) 1233, 2459, 4325
GsaI CCCAGC 2 cut(s) 3342, 3525
GsuI CTGGAG 3 cut(s) 2006, 2352, 3606
HaeII RGCGCY 1 cut(s) 2461
HaeIII GGCC 7 cut(s) 723, 1258, 1844, 2552, 3139, 3305, 3738
HgaI GACGC 2 cut(s) 1094, 2435
HhaI GCGC 3 cut(s) 1234, 2460, 4326
Hin6I GCGC 3 cut(s) 1232, 2458, 4324
HinP1I GCGC 3 cut(s) 1232, 2458, 4324
HincII GTYRAC 4 cut(s) 195, 2725, 4060, 4099
HindII GTYRAC 4 cut(s) 195, 2725, 4060, 4099
HindIII AAGCTT 4 cut(s) 84, 708, 2644, 2747
HpaI GTTAAC 1 cut(s) 4060
Hpy99I CGWCG 1 cut(s) 3137
HpyCH4III ACNGT 9 cut(s) 28, 96, 545, 1567, 1809, 2855, 3718, 4057, 4275
HpyCH4IV ACGT 7 cut(s) 213, 303, 782, 1328, 2480, 2599, 3624
HpySE526I ACGT 7 cut(s) 213, 303, 782, 1328, 2480, 2599, 3624
HspAI GCGC 3 cut(s) 1232, 2458, 4324
Kpn2I TCCGGA 2 cut(s) 1702, 2188
KpnI GGTACC 2 cut(s) 956, 3529
Ksp22I TGATCA 2 cut(s) 427, 1879
KspAI GTTAAC 1 cut(s) 4060
LguI GCTCTTC 5 cut(s) 178, 238, 1080, 1422, 2784
LmnI GCTCC 5 cut(s) 457, 869, 2371, 3023, 3716
LweI GCATC 8 cut(s) 433, 685, 1387, 1396, 3202, 3310, 3332, 3993
MaeII ACGT 7 cut(s) 213, 303, 782, 1328, 2480, 2599, 3624
MbiI CCGCTC 1 cut(s) 872
MfeI CAATTG 3 cut(s) 971, 3653, 3663
MflI RGATCY 4 cut(s) 229, 610, 3373, 3782
MhlI GDGCHC 3 cut(s) 332, 536, 3020
MlyI GAGTC 6 cut(s) 214, 635, 1136, 1879, 2302, 4322
MmeI TCCRAC 4 cut(s) 728, 847, 4180, 4286
Mph1103I ATGCAT 2 cut(s) 3217, 3325
MroI TCCGGA 2 cut(s) 1702, 2188
MroXI GAANNNNTTC 1 cut(s) 1219
MslI CAYNNNNRTG 7 cut(s) 441, 1245, 1322, 1353, 1819, 3147, 3318
MspA1I CMGCKG 4 cut(s) 1526, 2333, 2546, 3461
MspCI CTTAAG 1 cut(s) 3799
MunI CAATTG 3 cut(s) 971, 3653, 3663
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 5 cut(s) 448, 1744, 2370, 3387, 3585
MvnI CGCG 6 cut(s) 632, 2311, 3308, 3822, 4324, 4326
NciI CCSGG 9 cut(s) 47, 48, 74, 190, 261, 2690, 3530, 3531, 3684
NdeI CATATG 2 cut(s) 777, 2325
NmeAIII GCCGAG 2 cut(s) 1416, 2029
NmuCI GTSAC 8 cut(s) 451, 638, 1106, 1809, 2027, 2446, 4254, 4269
NsiI ATGCAT 2 cut(s) 3217, 3325
NspI RCATGY 5 cut(s) 739, 2127, 2717, 3270, 4001
NspV TTCGAA 1 cut(s) 2341
PaeI GCATGC 1 cut(s) 4001
PagI TCATGA 1 cut(s) 3841
PciI ACATGT 2 cut(s) 735, 2123
PciSI GCTCTTC 5 cut(s) 178, 238, 1080, 1422, 2784
PctI GAATGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 1219
PfeI GAWTC 4 cut(s) 758, 2980, 3063, 4192
PflMI CCANNNNNTGG 2 cut(s) 3901, 3980
PfoI TCCNGGA 2 cut(s) 2368, 3583
PleI GAGTC 6 cut(s) 213, 635, 1136, 1878, 2301, 4322
PpsI GAGTC 6 cut(s) 213, 635, 1136, 1878, 2301, 4322
Ppu21I YACGTR 2 cut(s) 2600, 3625
PscI ACATGT 2 cut(s) 735, 2123
PshBI ATTAAT 2 cut(s) 989, 2739
PsiI TTATAA 2 cut(s) 540, 1643
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 5 cut(s) 446, 1742, 2368, 3385, 3583
PspEI GGTNACC 1 cut(s) 4254
PspFI CCCAGC 2 cut(s) 3338, 3521
PspGI CCWGG 5 cut(s) 446, 1742, 2368, 3385, 3583
PspPI GGNCC 6 cut(s) 43, 661, 1256, 3137, 3303, 3434
PstI CTGCAG 1 cut(s) 3160
PstNI CAGNNNCTG 1 cut(s) 2227
PsuI RGATCY 4 cut(s) 229, 610, 3373, 3782
PvuII CAGCTG 4 cut(s) 1526, 2333, 2546, 3461
RseI CAYNNNNRTG 7 cut(s) 441, 1245, 1322, 1353, 1819, 3147, 3318
SapI GCTCTTC 5 cut(s) 178, 238, 1080, 1422, 2784
Sau96I GGNCC 6 cut(s) 43, 661, 1256, 3137, 3303, 3434
ScaI AGTACT 1 cut(s) 1273
SchI GAGTC 6 cut(s) 214, 635, 1136, 1879, 2302, 4322
SduI GDGCHC 3 cut(s) 332, 536, 3020
SfaNI GCATC 8 cut(s) 433, 685, 1387, 1396, 3202, 3310, 3332, 3993
SfcI CTRYAG 4 cut(s) 342, 1123, 3156, 3864
SfuI TTCGAA 1 cut(s) 2341
SinI GGWCC 3 cut(s) 43, 661, 3434
SmaI CCCGGG 2 cut(s) 48, 3531
SmiMI CAYNNNNRTG 7 cut(s) 441, 1245, 1322, 1353, 1819, 3147, 3318
SmlI CTYRAG 5 cut(s) 957, 2750, 2933, 3420, 3799
SmoI CTYRAG 5 cut(s) 957, 2750, 2933, 3420, 3799
SnaBI TACGTA 1 cut(s) 2600
SpeI ACTAGT 2 cut(s) 1613, 4295
SphI GCATGC 1 cut(s) 4001
SspI AATATT 2 cut(s) 3513, 4346
StyI CCWWGG 5 cut(s) 1664, 2044, 3429, 3673, 4187
TaaI ACNGT 9 cut(s) 28, 96, 545, 1567, 1809, 2855, 3718, 4057, 4275
TaiI ACGT 7 cut(s) 216, 306, 785, 1331, 2483, 2602, 3627
TaqII GACCGA 2 cut(s) 1401, 2940
TatI WGTACW 6 cut(s) 171, 1237, 1271, 1508, 1646, 3831
TauI GCSGC 4 cut(s) 398, 3308, 3822, 3992
TfiI GAWTC 4 cut(s) 758, 2980, 3063, 4192
TscAI CASTG 6 cut(s) 99, 529, 1572, 2151, 3444, 3821
TseFI GTSAC 8 cut(s) 451, 638, 1106, 1809, 2027, 2446, 4254, 4269
Tsp45I GTSAC 8 cut(s) 451, 638, 1106, 1809, 2027, 2446, 4254, 4269
TspGWI ACGGA 7 cut(s) 266, 470, 633, 734, 1329, 1470, 1770
TspMI CCCGGG 2 cut(s) 46, 3529
TspRI CASTG 6 cut(s) 99, 529, 1572, 2151, 3444, 3821
Van91I CCANNNNNTGG 2 cut(s) 3901, 3980
Vha464I CTTAAG 1 cut(s) 3799
VpaK11BI GGWCC 3 cut(s) 43, 661, 3434
VspI ATTAAT 2 cut(s) 989, 2739
XapI RAATTY 8 cut(s) 2020, 2181, 2343, 2379, 2392, 2555, 3698, 4305
XbaI TCTAGA 2 cut(s) 356, 2104
XceI RCATGY 5 cut(s) 739, 2127, 2717, 3270, 4001
XmaI CCCGGG 2 cut(s) 46, 3529
XmaJI CCTAGG 1 cut(s) 3673
XmiI GTMKAC 2 cut(s) 408, 1122
XmnI GAANNNNTTC 1 cut(s) 1219
ZrmI AGTACT 1 cut(s) 1273
Zsp2I ATGCAT 2 cut(s) 3217, 3325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.