Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0010177

Physical Location & Seq

Genomic Coordinates Reverse (-)

9128174 .. 9131806

Gene Structure 3633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG099500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2397 bp

ATGGCTACCATTGTCAGTTTGCTCGTTTATTTTGCCATTGCCTCTTCTTTCTTCGTTTCTTCTAATGCTGGAAGAAAACTTCTCGACTCTACAAGTGTTTCCACCAATGGAGCCACTAACTTTACCATTGTGTGTGATCCGTCAAGGTACTCCAACCTCGGCCTGAACATATCTGACTTTGCCTTCTGCGACTCAGCCTTGCCGTACGATGCTAGGGTCAAAGACTTGGTGGATAGGATGACCTTGGCAGAGAAAGTGCAGCAGCTAGGAAACAAAGCAGCTGGGGTGCCTAGACTAGGCTTGCCCAAGTACGAGTGGTGGTCTGAGGCACTCCACGGTGTATCCAACGTCGGTCCGGGTACGTACTTTGATGATGTTGTTCCCGGTGCAACAAGCTTCCCCCCTGTCATTCTCACCGCCGCGTCATTCAACGAGTCCTTGTGGAAGGAGATTGGTCAGGTTGTTTCAACAGAAGCAAGAGCCATGTACAACCTAGGAAGGGCTGGATTGACATATTGGAGTCCCAATATAAATGTAGTGAGGGATCCAAGGTGGGGAAGAGCCACAGAGACGCCCGGTGAAGACCCTTATGTTGTTGGAGCTTATGCTATAAACTATGTCCGAGGCTTGCAAGATGTTAAGGGTACAGAGAATTTTGCAGACTTGAACTCTAGGCCTCTTAAGGTCTCCTCTTGCTGCAAGCACTATGCTGCCTATGATGTTGATAACTGGCTTGGTGTGGATCGTTACCATTTTGATGCCCAGGTTACGGAGCAAGATATGTCAGAGACTTTCCTTAAACCATTTGAGATGTGTGTTAAAGATGGTGATGTTAGTAGTGTAATGTGCTCCTACAACAGGATTAATGGAATACCAGCTTGTGCTGATACAAAGCTCTTGAAAGATACCATTAGAGGAGAATGGGACCTTCATGGATACATTGTCTCAGATTGTGACTCTCTAGAAGTAATGGTGAATGGCCATAAATGGCTTGGTGACACTGGAGTGGAAGCTTCTGCACAAGCATTAAAAGCAGGGATGGATTTGGACTGTGGAATCTACTACCCTAATTTCACTGAGTCTGCTGTGAAGCAAGGGAAGGTGAAGGTCAAAGAAATAGACAATTCTTTGAAGCAGCTTTATGTGGTACTTATGAGGCTAGGCTTTTTTGATGGAAACCCTATTTACAACTCACTTGGCAAGAAAGATGTGTGCTCCCAAGAACACATTGAATTGGCAAGAGAAGCAGCCAAAGAAGGAATTGTTTTACTAAAAAATGCCAATGAAACTTTGCCTGTCAACTCTTACAAGATCAAGAAATTGGCCGTGGTCGGGCCTCATGCCAATGCTACTACAGCTATGATTGGAAATTATGCAGGTATTCCTTGCAAGTTCATCTCACCTCTTGATGCTTTTTCGAAATATGGAGAAGTGAAGTATGAAGTGGGATGTGATGTTAAGTGTCCAAATGAGAGCCTGATATTCCCAGCCATGCAAGCTGCAAAGGAAGCTGATGTGACAATAATTTTTGCTGGATTAGACTTATCAGTTGAGGCTGAGAGCTTGGACAGGACTGATCTTCTTCTCCCTGGGTATCAAACCCAATTCATCAACCAAGTTGCTCAAGTATCAAAAGGTCCGGTAATTCTTGTGATCTTGTCTGCAGGGGGTGTTGATATCACCTTTGCCAAGAACAATGATAACATCAAAGCCATTTTGTGGGCTGGATACCCTGGTGAGCAAGGTGGCCGTGCCATTGCAGATGTTGTTTTTGGACATTACAATCCTGGTGGAAGATTACCTCTTACATGGTACCCAGCTGATTATGTGGATATGCTACCCTTAACCTCCATGCCATTGAGGCCAATTGATAGCTTAGGATATCCTGGTCGTACATACAAGTTCTACAATGGATCCACAGTTTACCCATTTGGATATGGTCTTAGCTACACCAAATTCAACTACACTATACTATCTGCAAAGAGATCTGTAGACATAAAGTTGGAGAAGACCCAACACTGCCGTGACTTGGAGTACAAACAAGGGGAAAAGAAACCAGCTTGCCCTTCAGTCCTTATTGATGACTTGGACTGCCAATATGATTTCGAGTTTGAGGTTGAAGTCCGAAATGTGGGCGGAAGGGATGGAAGTGAAGTCATTTTAGTTTACTCTAAACCCCCAAGTGGCATTGCCTCTACACATGCTAAGCAGGTTATTGGGTTTCAGAGGGTGTTTGTTGAAGCTGGCAAGACTGAGAAAGTCAAATTTGTCTTCAATGTTTACAAGAGCTTGGGAATTATTGATTATACAGCTTATAATCTTTTACCATCAGGCAGCCACACCATTTCGCTCGGAGACGATCTGGTTACATTCCCTCTCCAAGTTAACCTTGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

798

Amino Acids

87.68

Weight (kDa)

5.39

Isoelectric Point (pI)

29.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	129 - 380	4.5e-35	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	421 - 650	7.4e-50	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	718 - 786	3.5e-09	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09700
fragaria_vesca	FvH4_3g05990
malus_domestica	MD05G1287500.v1.1 MD05G1287700.v1.1 MD05G1288600.v1.1 MD10G1264800.v1.1 MD17G1089800.v1.1
prunus_persica	Prupe.4G077200_v2.0.a1
pyrus_communis	pycom05g26600
rosa_chinensis	RchiOBHm_Chr5g0013421
rosa_laevigata	RLG00000032018
rosa_multiflora	Rmu_sc0003458.1_g000016 Rmu_sc0003458.1_g000017
rosa_roxburghii	Rroxscaffold_1G00063140
rosa_rugosa	Rorug05G0009200 Rorug05G0009300
rosa_samantha	Rh5AG104600 Rh5BG100900 Rh5CG112800 Rh5DG099500
rosa_wichuraiana	Rw5G009120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2316
Acc36I	ACCTGC	2 cut(s)	1367, 2200
Acc65I	GGTACC	1 cut(s)	1812
AccB1I	GGYRCC	2 cut(s)	286, 1812
AccB7I	CCANNNNNTGG	1 cut(s)	1719
AccI	GTMKAC	1 cut(s)	1992
AccII	CGCG	1 cut(s)	422
AciI	CCGC	3 cut(s)	417, 420, 2136
AclWI	GGATC	6 cut(s)	131, 539, 552, 750, 1908, 1921
AcoI	YGGCCR	3 cut(s)	979, 1323, 1747
AcsI	RAATTY	3 cut(s)	652, 1955, 2264
AcuI	CTGAAG	1 cut(s)	2052
AcyI	GRCGYC	1 cut(s)	572
AflII	CTTAAG	1 cut(s)	680
AjnI	CCWGG	5 cut(s)	762, 1588, 1732, 1786, 1885
AleI	CACNNNNGTG	2 cut(s)	1004, 2022
Alw21I	GWGCWC	2 cut(s)	851, 1217
Alw26I	GTCTC	5 cut(s)	563, 691, 782, 949, 2349
AlwI	GGATC	6 cut(s)	131, 539, 552, 750, 1908, 1921
AoxI	GGCC	7 cut(s)	160, 674, 979, 1323, 1334, 1747, 1862
ApeKI	GCWGC	9 cut(s)	259, 262, 278, 696, 710, 1135, 1247, 1499, 2334
ApoI	RAATTY	3 cut(s)	652, 1955, 2264
AseI	ATTAAT	1 cut(s)	864
Asp718I	GGTACC	1 cut(s)	1812
AspA2I	CCTAGG	1 cut(s)	493
AspS9I	GGNCC	4 cut(s)	353, 925, 1334, 1637
AsuC2I	CCSGG	3 cut(s)	357, 384, 576
AsuHPI	GGTGA	9 cut(s)	406, 590, 839, 985, 1007, 1114, 1392, 1672, 1748
AsuII	TTCGAA	1 cut(s)	1418
AvaII	GGWCC	3 cut(s)	353, 925, 1637
AvrII	CCTAGG	1 cut(s)	493
BaeI	ACNNNNGTAYC	2 cut(s)	1720, 1753
BalI	TGGCCA	1 cut(s)	981
BamHI	GGATCC	2 cut(s)	544, 1913
BanI	GGYRCC	2 cut(s)	286, 1812
BbsI	GAAGAC	3 cut(s)	588, 2015, 2263
Bbv12I	GWGCWC	2 cut(s)	851, 1217
BbvI	GCAGC	9 cut(s)	271, 274, 290, 683, 697, 1147, 1259, 1486, 2346
BccI	CCATC	5 cut(s)	818, 1033, 1166, 2138, 2335
BceAI	ACGGC	4 cut(s)	187, 1310, 1734, 2007
BciT130I	CCWGG	5 cut(s)	764, 1590, 1734, 1788, 1887
BciVI	GTATCC	3 cut(s)	352, 929, 1721
BcnI	CCSGG	3 cut(s)	357, 384, 576
BcoDI	GTCTC	5 cut(s)	563, 691, 782, 949, 2349
BfaI	CTAG	8 cut(s)	213, 266, 291, 296, 494, 672, 962, 1160
BfmI	CTRYAG	3 cut(s)	1353, 1662, 1989
BfrI	CTTAAG	1 cut(s)	680
BfuAI	ACCTGC	2 cut(s)	1367, 2200
BfuI	GTATCC	3 cut(s)	352, 929, 1721
BglI	GCCNNNNNGGC	1 cut(s)	1861
BglII	AGATCT	1 cut(s)	1985
BlnI	CCTAGG	1 cut(s)	493
BlpI	GCTNAGC	1 cut(s)	2205
Bme1390I	CCNGG	8 cut(s)	357, 384, 576, 764, 1590, 1734, 1788, 1887
Bme18I	GGWCC	3 cut(s)	353, 925, 1637
BmgT120I	GGNCC	4 cut(s)	353, 925, 1334, 1637
BmiI	GGNNCC	6 cut(s)	112, 288, 546, 926, 1814, 1915
BmrFI	CCNGG	8 cut(s)	357, 384, 576, 764, 1590, 1734, 1788, 1887
BmsI	GCATC	3 cut(s)	199, 748, 1399
BpiI	GAAGAC	3 cut(s)	588, 2015, 2263
BpmI	CTGGAG	1 cut(s)	1023
Bpu10I	CCTNAGC	1 cut(s)	1876
Bpu1102I	GCTNAGC	1 cut(s)	2205
Bpu14I	TTCGAA	1 cut(s)	1418
BpuEI	CTTGAG	1 cut(s)	1608
BpuMI	CCSGG	3 cut(s)	357, 384, 576
BsaAI	YACGTR	1 cut(s)	363
BsaHI	GRCGYC	1 cut(s)	572
BsaI	GGTCTC	1 cut(s)	691
BsaWI	WCCGGW	1 cut(s)	1639
Bse1I	ACTGG	2 cut(s)	734, 1006
Bse3DI	GCAATG	3 cut(s)	36, 1755, 2187
BseBI	CCWGG	5 cut(s)	764, 1590, 1734, 1788, 1887
BseGI	GGATG	4 cut(s)	243, 1044, 1454, 2149
BseMI	GCAATG	3 cut(s)	36, 1755, 2187
BseMII	CTCAG	6 cut(s)	207, 315, 960, 1068, 1548, 2244
BseNI	ACTGG	2 cut(s)	734, 1006
BseRI	GAGGAG	2 cut(s)	679, 930
BseXI	GCAGC	9 cut(s)	271, 274, 290, 683, 697, 1147, 1259, 1486, 2346
BseYI	CCCAGC	3 cut(s)	281, 1486, 1816
BsgI	GTGCAG	2 cut(s)	278, 1002
Bsh1236I	CGCG	1 cut(s)	422
BshFI	GGCC	7 cut(s)	162, 676, 981, 1325, 1336, 1749, 1864
BshNI	GGYRCC	2 cut(s)	286, 1812
BsiHKAI	GWGCWC	2 cut(s)	851, 1217
BsiSI	CCGG	4 cut(s)	356, 384, 576, 1640
BsiWI	CGTACG	1 cut(s)	204
BslFI	GGGAC	2 cut(s)	507, 938
BsmAI	GTCTC	5 cut(s)	563, 691, 782, 949, 2349
BsmBI	CGTCTC	2 cut(s)	563, 2349
BsmFI	GGGAC	2 cut(s)	507, 938
BsnI	GGCC	7 cut(s)	162, 676, 981, 1325, 1336, 1749, 1864
Bso31I	GGTCTC	1 cut(s)	691
Bsp119I	TTCGAA	1 cut(s)	1418
Bsp1286I	GDGCHC	2 cut(s)	851, 1217
Bsp1407I	TGTACA	1 cut(s)	486
Bsp143I	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
Bsp1720I	GCTNAGC	1 cut(s)	2205
BspACI	CCGC	3 cut(s)	417, 420, 2136
BspANI	GGCC	7 cut(s)	162, 676, 981, 1325, 1336, 1749, 1864
BspCNI	CTCAG	6 cut(s)	206, 316, 959, 1069, 1549, 2245
BspFNI	CGCG	1 cut(s)	422
BspLI	GGNNCC	6 cut(s)	112, 288, 546, 926, 1814, 1915
BspMAI	CTGCAG	1 cut(s)	1666
BspMI	ACCTGC	2 cut(s)	1367, 2200
BspPI	GGATC	6 cut(s)	131, 539, 552, 750, 1908, 1921
BspQI	GCTCTTC	1 cut(s)	553
BspT104I	TTCGAA	1 cut(s)	1418
BspT107I	GGYRCC	2 cut(s)	286, 1812
BspTI	CTTAAG	1 cut(s)	680
BspTNI	GGTCTC	1 cut(s)	691
BsrDI	GCAATG	3 cut(s)	36, 1755, 2187
BsrGI	TGTACA	1 cut(s)	486
BsrI	ACTGG	2 cut(s)	734, 1006
BssMI	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
BssNI	GRCGYC	1 cut(s)	572
BssT1I	CCWWGG	3 cut(s)	243, 493, 548
Bst2UI	CCWGG	5 cut(s)	764, 1590, 1734, 1788, 1887
Bst4CI	ACNGT	3 cut(s)	338, 1052, 1921
Bst6I	CTCTTC	2 cut(s)	49, 553
BstACI	GRCGYC	1 cut(s)	572
BstAFI	CTTAAG	1 cut(s)	680
BstAUI	TGTACA	1 cut(s)	486
BstBAI	YACGTR	1 cut(s)	363
BstBI	TTCGAA	1 cut(s)	1418
BstC8I	GCNNGC	6 cut(s)	302, 629, 701, 1497, 2062, 2245
BstDEI	CTNAG	9 cut(s)	193, 324, 946, 1077, 1557, 1876, 1943, 2205, 2253
BstDSI	CCRYGG	2 cut(s)	334, 1326
BstENI	CCTNNNNNAGG	2 cut(s)	294, 856
BstF5I	GGATG	4 cut(s)	243, 1044, 1454, 2149
BstFNI	CGCG	1 cut(s)	422
BstKTI	GATC	9 cut(s)	139, 547, 745, 1314, 1579, 1656, 1916, 1988, 2362
BstMAI	GTCTC	5 cut(s)	563, 691, 782, 949, 2349
BstMBI	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
BstMWI	GCNNNNNNNGC	6 cut(s)	1031, 1244, 1355, 1496, 1508, 1861
BstNI	CCWGG	5 cut(s)	764, 1590, 1734, 1788, 1887
BstNSI	RCATGY	1 cut(s)	2204
BstSCI	CCNGG	8 cut(s)	355, 382, 574, 762, 1588, 1732, 1786, 1885
BstSFI	CTRYAG	3 cut(s)	1353, 1662, 1989
BstSNI	TACGTA	1 cut(s)	363
BstUI	CGCG	1 cut(s)	422
BstV1I	GCAGC	9 cut(s)	271, 274, 290, 683, 697, 1147, 1259, 1486, 2346
BstV2I	GAAGAC	3 cut(s)	588, 2015, 2263
BstX2I	RGATCY	3 cut(s)	544, 1913, 1985
BstYI	RGATCY	3 cut(s)	544, 1913, 1985
BsuI	GTATCC	3 cut(s)	352, 929, 1721
BsuRI	GGCC	7 cut(s)	162, 676, 981, 1325, 1336, 1749, 1864
BtgI	CCRYGG	2 cut(s)	334, 1326
BtsCI	GGATG	4 cut(s)	243, 1044, 1454, 2149
BtsI	GCAGTG	1 cut(s)	2017
BtsIMutI	CAGTG	3 cut(s)	999, 1074, 2017
BveI	ACCTGC	2 cut(s)	1367, 2200
Cac8I	GCNNGC	6 cut(s)	302, 629, 701, 1497, 2062, 2245
Cfr13I	GGNCC	4 cut(s)	353, 925, 1334, 1637
CpoI	CGGWCCG	1 cut(s)	353
CseI	GACGC	2 cut(s)	411, 580
CspCI	CAANNNNNGTGG	2 cut(s)	1771, 1806
CspI	CGGWCCG	1 cut(s)	353
CviAII	CATG	7 cut(s)	484, 932, 1340, 1492, 1809, 1852, 2201
DdeI	CTNAG	9 cut(s)	193, 324, 946, 1077, 1557, 1876, 1943, 2205, 2253
DpnI	GATC	9 cut(s)	138, 546, 744, 1313, 1578, 1655, 1915, 1987, 2361
DpnII	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
EaeI	YGGCCR	3 cut(s)	979, 1323, 1747
Eam1104I	CTCTTC	2 cut(s)	49, 553
EarI	CTCTTC	2 cut(s)	49, 553
EciI	GGCGGA	1 cut(s)	2151
Eco105I	TACGTA	1 cut(s)	363
Eco130I	CCWWGG	3 cut(s)	243, 493, 548
Eco147I	AGGCCT	1 cut(s)	676
Eco31I	GGTCTC	1 cut(s)	691
Eco32I	GATATC	2 cut(s)	1678, 1883
Eco47I	GGWCC	3 cut(s)	353, 925, 1637
Eco57I	CTGAAG	1 cut(s)	2052
EcoNI	CCTNNNNNAGG	2 cut(s)	294, 856
EcoO109I	RGGNCCY	1 cut(s)	925
EcoRII	CCWGG	5 cut(s)	762, 1588, 1732, 1786, 1885
EcoRV	GATATC	2 cut(s)	1678, 1883
EcoT14I	CCWWGG	3 cut(s)	243, 493, 548
ErhI	CCWWGG	3 cut(s)	243, 493, 548
Esp3I	CGTCTC	2 cut(s)	563, 2349
FaeI	CATG	7 cut(s)	487, 935, 1343, 1495, 1812, 1855, 2204
FalI	AAGNNNNNCTT	1 cut(s)	2373
FaqI	GGGAC	2 cut(s)	507, 938
FatI	CATG	7 cut(s)	483, 931, 1339, 1491, 1808, 1851, 2200
FblI	GTMKAC	1 cut(s)	1992
FokI	GGATG	4 cut(s)	250, 1051, 1461, 2156
FspBI	CTAG	8 cut(s)	213, 266, 291, 296, 494, 672, 962, 1160
GsaI	CCCAGC	3 cut(s)	285, 1490, 1820
GsuI	CTGGAG	1 cut(s)	1023
HaeIII	GGCC	7 cut(s)	162, 676, 981, 1325, 1336, 1749, 1864
HapII	CCGG	4 cut(s)	356, 384, 576, 1640
HgaI	GACGC	2 cut(s)	411, 580
Hin1I	GRCGYC	1 cut(s)	572
Hin1II	CATG	7 cut(s)	487, 935, 1343, 1495, 1812, 1855, 2204
HincII	GTYRAC	2 cut(s)	1300, 2386
HindII	GTYRAC	2 cut(s)	1300, 2386
HindIII	AAGCTT	2 cut(s)	394, 1011
HinfI	GANTC	7 cut(s)	86, 191, 434, 520, 956, 1056, 1079
HpaI	GTTAAC	1 cut(s)	2386
HpaII	CCGG	4 cut(s)	356, 384, 576, 1640
HphI	GGTGA	9 cut(s)	406, 590, 839, 985, 1007, 1114, 1392, 1672, 1748
Hpy166II	GTNNAC	6 cut(s)	1300, 1924, 1993, 2167, 2281, 2386
Hpy188I	TCNGA	8 cut(s)	175, 325, 623, 787, 949, 2126, 2226, 2354
Hpy188III	TCNNGA	5 cut(s)	83, 898, 962, 1315, 1406
Hpy8I	GTNNAC	6 cut(s)	1300, 1924, 1993, 2167, 2281, 2386
Hpy99I	CGWCG	1 cut(s)	353
HpyAV	CCTTC	9 cut(s)	193, 439, 492, 938, 1093, 1099, 1250, 2076, 2133
HpyCH4III	ACNGT	3 cut(s)	338, 1052, 1921
HpyCH4IV	ACGT	2 cut(s)	348, 362
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1031, 1244, 1355, 1496, 1508, 1861
HpyF3I	CTNAG	9 cut(s)	193, 324, 946, 1077, 1557, 1876, 1943, 2205, 2253
HpySE526I	ACGT	2 cut(s)	348, 362
Hsp92I	GRCGYC	1 cut(s)	572
Hsp92II	CATG	7 cut(s)	487, 935, 1343, 1495, 1812, 1855, 2204
KpnI	GGTACC	1 cut(s)	1816
KspAI	GTTAAC	1 cut(s)	2386
Kzo9I	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
LguI	GCTCTTC	1 cut(s)	553
LmnI	GCTCC	5 cut(s)	110, 599, 772, 854, 1220
Lsp1109I	GCAGC	9 cut(s)	271, 274, 290, 683, 697, 1147, 1259, 1486, 2346
LweI	GCATC	3 cut(s)	199, 748, 1399
MaeI	CTAG	8 cut(s)	213, 266, 291, 296, 494, 672, 962, 1160
MaeII	ACGT	2 cut(s)	348, 362
MaeIII	GTNAC	7 cut(s)	746, 766, 953, 995, 1516, 2024, 2365
MalI	GATC	9 cut(s)	138, 546, 744, 1313, 1578, 1655, 1915, 1987, 2361
MboI	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
MfeI	CAATTG	1 cut(s)	1866
MflI	RGATCY	3 cut(s)	544, 1913, 1985
MhlI	GDGCHC	2 cut(s)	851, 1217
MlsI	TGGCCA	1 cut(s)	981
MluNI	TGGCCA	1 cut(s)	981
MlyI	GAGTC	6 cut(s)	80, 185, 443, 529, 950, 1088
MmeI	TCCRAC	4 cut(s)	177, 369, 577, 1983
Mox20I	TGGCCA	1 cut(s)	981
MscI	TGGCCA	1 cut(s)	981
MseI	TTAA	9 cut(s)	639, 681, 798, 819, 864, 1028, 1458, 1844, 2385
MslI	CAYNNNNRTG	4 cut(s)	756, 1004, 1344, 2022
Msp20I	TGGCCA	1 cut(s)	981
MspA1I	CMGCKG	2 cut(s)	281, 1820
MspCI	CTTAAG	1 cut(s)	680
MspI	CCGG	4 cut(s)	356, 384, 576, 1640
MspR9I	CCNGG	8 cut(s)	357, 384, 576, 764, 1590, 1734, 1788, 1887
MunI	CAATTG	1 cut(s)	1866
MvaI	CCWGG	5 cut(s)	764, 1590, 1734, 1788, 1887
MvnI	CGCG	1 cut(s)	422
MwoI	GCNNNNNNNGC	6 cut(s)	1031, 1244, 1355, 1496, 1508, 1861
NciI	CCSGG	3 cut(s)	357, 384, 576
NdeII	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
NlaIII	CATG	7 cut(s)	487, 935, 1343, 1495, 1812, 1855, 2204
NlaIV	GGNNCC	6 cut(s)	112, 288, 546, 926, 1814, 1915
NmeAIII	GCCGAG	1 cut(s)	138
NmuCI	GTSAC	4 cut(s)	953, 995, 1516, 2024
NspI	RCATGY	1 cut(s)	2204
NspV	TTCGAA	1 cut(s)	1418
OliI	CACNNNNGTG	2 cut(s)	1004, 2022
PasI	CCCWGGG	1 cut(s)	1589
PceI	AGGCCT	1 cut(s)	676
PciSI	GCTCTTC	1 cut(s)	553
PfeI	GAWTC	1 cut(s)	1056
Pfl23II	CGTACG	1 cut(s)	204
PflMI	CCANNNNNTGG	1 cut(s)	1719
PleI	GAGTC	6 cut(s)	80, 185, 442, 528, 950, 1087
PpsI	GAGTC	6 cut(s)	80, 185, 442, 528, 950, 1087
Ppu21I	YACGTR	1 cut(s)	363
PpuMI	RGGWCCY	1 cut(s)	925
PshBI	ATTAAT	1 cut(s)	864
PsiI	TTATAA	1 cut(s)	2316
Psp5II	RGGWCCY	1 cut(s)	925
Psp6I	CCWGG	5 cut(s)	762, 1588, 1732, 1786, 1885
PspFI	CCCAGC	3 cut(s)	281, 1486, 1816
PspGI	CCWGG	5 cut(s)	762, 1588, 1732, 1786, 1885
PspLI	CGTACG	1 cut(s)	204
PspN4I	GGNNCC	6 cut(s)	112, 288, 546, 926, 1814, 1915
PspPI	GGNCC	4 cut(s)	353, 925, 1334, 1637
PspPPI	RGGWCCY	1 cut(s)	925
PstI	CTGCAG	1 cut(s)	1666
PsuI	RGATCY	3 cut(s)	544, 1913, 1985
PvuII	CAGCTG	2 cut(s)	281, 1820
RseI	CAYNNNNRTG	4 cut(s)	756, 1004, 1344, 2022
Rsr2I	CGGWCCG	1 cut(s)	353
RsrII	CGGWCCG	1 cut(s)	353
SapI	GCTCTTC	1 cut(s)	553
SaqAI	TTAA	9 cut(s)	639, 681, 798, 819, 864, 1028, 1458, 1844, 2385
Sau3AI	GATC	9 cut(s)	136, 544, 742, 1311, 1576, 1653, 1913, 1985, 2359
Sau96I	GGNCC	4 cut(s)	353, 925, 1334, 1637
SchI	GAGTC	6 cut(s)	80, 185, 443, 529, 950, 1088
ScrFI	CCNGG	8 cut(s)	357, 384, 576, 764, 1590, 1734, 1788, 1887
SduI	GDGCHC	2 cut(s)	851, 1217
SfaNI	GCATC	3 cut(s)	199, 748, 1399
SfcI	CTRYAG	3 cut(s)	1353, 1662, 1989
SfuI	TTCGAA	1 cut(s)	1418
SinI	GGWCC	3 cut(s)	353, 925, 1637
SmiMI	CAYNNNNRTG	4 cut(s)	756, 1004, 1344, 2022
SmlI	CTYRAG	2 cut(s)	680, 1623
SmoI	CTYRAG	2 cut(s)	680, 1623
SnaBI	TACGTA	1 cut(s)	363
SseBI	AGGCCT	1 cut(s)	676
SsiI	CCGC	3 cut(s)	417, 420, 2136
SspMI	CTAG	8 cut(s)	213, 266, 291, 296, 494, 672, 962, 1160
StuI	AGGCCT	1 cut(s)	676
StyD4I	CCNGG	8 cut(s)	355, 382, 574, 762, 1588, 1732, 1786, 1885
StyI	CCWWGG	3 cut(s)	243, 493, 548
TaaI	ACNGT	3 cut(s)	338, 1052, 1921
TaiI	ACGT	2 cut(s)	351, 365
TaqI	TCGA	3 cut(s)	84, 1418, 2106
TaqII	GACCGA	1 cut(s)	341
TatI	WGTACW	2 cut(s)	486, 2034
TauI	GCSGC	1 cut(s)	422
TfiI	GAWTC	1 cut(s)	1056
Tru1I	TTAA	9 cut(s)	639, 681, 798, 819, 864, 1028, 1458, 1844, 2385
Tru9I	TTAA	9 cut(s)	639, 681, 798, 819, 864, 1028, 1458, 1844, 2385
TscAI	CASTG	3 cut(s)	1006, 1081, 2024
TseFI	GTSAC	4 cut(s)	953, 995, 1516, 2024
TseI	GCWGC	9 cut(s)	259, 262, 278, 696, 710, 1135, 1247, 1499, 2334
Tsp45I	GTSAC	4 cut(s)	953, 995, 1516, 2024
TspDTI	ATGAA	5 cut(s)	920, 1299, 1384, 1455, 1597
TspGWI	ACGGA	2 cut(s)	129, 785
TspRI	CASTG	3 cut(s)	1006, 1081, 2024
Van91I	CCANNNNNTGG	1 cut(s)	1719
Vha464I	CTTAAG	1 cut(s)	680
VpaK11BI	GGWCC	3 cut(s)	353, 925, 1637
VspI	ATTAAT	1 cut(s)	864
XagI	CCTNNNNNAGG	2 cut(s)	294, 856
XapI	RAATTY	3 cut(s)	652, 1955, 2264
XbaI	TCTAGA	1 cut(s)	961
XceI	RCATGY	1 cut(s)	2204
XcmI	CCANNNNNNNNNTGG	1 cut(s)	989
XmaJI	CCTAGG	1 cut(s)	493
XmiI	GTMKAC	1 cut(s)	1992
XspI	CTAG	8 cut(s)	213, 266, 291, 296, 494, 672, 962, 1160

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.