MD05G1287700.v1.1

beta-xylosidase alpha-L-arabinofuranosidase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010177
Physical Location & Seq
Forward (+)
42029425 .. 42036819
7395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1287700.v1.1.491

Sequence Viewer

Length: 4158 bp
ATGAGTGGTGGTCTGAGGCACTCCACGGTGTGTCAAATGTTGGTCCCGGGACTTATTTCGATGATCTTATTCCGGGTGCAACAAGCTTCCCCACTGTTATTCTTACCACCGCAGCGTTCAACCAGTCTTTGTGGAAGCAAATTGGTTGTTTCAACAGAAGCAAGAGCCATGTACAATCTAGGAAGAGCCGGGTTGACATATTGGAGTCCCAACGTTAATGTAGTGAGGGATCCGAGGTGGGGAAGAGCCACGGAAACACCCGGTGAAGACCCTTATGTTGTTGGCGTTTATGCCTCTAACTACGTCAGAGGCTTGCAGGATGTTGAGGGCACAGATAATGCTACAGATTTGAACTCTAGATCTCTCAAAGTTTCCTCATGCTGCAAACATTATGCCGCCTACGATGTAGACAACTGGCTCGGTGTTGATCATTACCATTTTGATGCCAGGGATGGTGATGTAAGCAGTGTAATGTGCTCTTATAACCGTGTTAATGGCATACCTACTTGTGCTGATCCGAAACTCTTGACACATACCATTAGAGGAGAGTGGGATCTTCACGGATATATAGTCGCGGATTGTGACTCTCTTGAAGTTATGGTGGACCACCACAAATGGCTCAGCGACACCAATGAGGATGCTGCTGCACAAGCTTTGAAAGCAGGCCATAACTTCAAGTTTGTAACAGGGCTATCTAACGTGTTTTACTGCTGTCTTCAGTGCGGCTATAATCAAAATTGTCGTTGGATTTTGGATTCTGGTGCAACAGATCATATGACGTATGATAAAACCTTGTTTCAATCTATGACACATCCTCATAGAAAGTGTGTGGCAACCGCCAATGGTTCTACTGCTGTTGTTGTTGGAGCGGGAACAGTGTCTCTTACACCCTCTCTTCCCATACATAATTGTTTATTGGTTCCGTCATTGTCTCATCACTTATTGTCGGTACCTCAAGTCACTGAACAATTGGATTGTGTTGTATTAATGTATCCTTCATTTTGCTTGCTTCAGGATATTCAGACAAAGGAGATCATTGGGCGTGGCACTAAGAGAAATGGGCTATATTATGTGGATGATGTGGTTCTTGGAAGAGCTAATTTGGTTCATGCATCACGTAATAGTAGTCTACAGAATGTATTGTTATTGCATCGTCATTTGGGGCATGCATCGTTTGGTTATTTGAAACATATATTACCTGATTTGTTCAGTGGTATTGAAGACTCAGAATTGAAATGTGAAGTATGTATTTTAGCCAAAAGTCATCGTGCTTCATTCCCTCCAAGTATGAATAGAACATCTTCTCCATTTGCGCTAGTACACTCTGATGTGTGGGGGCCTTCCCTTATTAGTACTACTTCGAGAATCAAATGGTTTATTACATTTGTTAATGATTGCACTCGTATGACGTGGGTTTATCTGTTGAAACATAAAAGTGATTCCTCCGTTATTAAGGTTCTTCGTACTGATAATGGGGGAGAATATGTTAACTCTGAGCTGTCTACTTTTTTCCGTGATCAAGGAATTCTTCATGAGACAACGTGTTTGTATACCCCACAACAGAATGGGGTAGCTGAGAGAAAAAACGGTCATATTTTGGAAACTGCTCATGCATTGCTTATTGGTGGGTCTGTGCCCAAAAGTTTTTGGCTAGACGCCATCACCTATGCCGTGTATGTTATAAATCGCATGCCATCCCGGGTTGTAAAATTTCGTACTCCACTCCAAGTGTTGACGGAACATGTTCCAGTTGTTTCTACCAATACTCTCACTCCTCGAGTATTAGGATGCGTTGCTTATGTTCATATTCACAAGATTCATCGTAGTAAATTGGATCCTTGTGCTCTTCGGTGTGTCTTTGTGGGTTTTGCATCCCACCAGAAAGGTTACAAGTGTTACCATCCTACAACTCGCCACATGTATATAACCATGGATGTGACTTTCTCTGAGTCAGAATATTTTTATACTCCCGTATCACCACCCTTCGATCACAAAGGGGAGAGTTATAGTTGTGATCTTGATAGTGTTTTTGAATGGTTGGATGTACAAGAGGTAACGTTTTCAGGGGGAGTGCATGGTGCCGTGCCAAGTGATATAGTTGATGCTCGGTCTCCTTCTGTTTTAAGAGCCTGTTGTGAGTGGTCAGCATCTTTTCACCGAAGAATAGGCTGCCCATCAAGTGTGTTGGTGTTGCCAAAGAGGTTTGTACTGATGGTCAGCCAGCTGTTGTCGAAGCAGCTTCTTCTCCTTTCTCTAGCTCAACAGTGCTGTTGTCCAATACATCTTCTCTGGATATCCCTGAGGTATAGCAACGGAGGAAAAATGGGAGTGTGCCGACTGCGGAAGTCCCTTTATGGGCTTAAACAGTCACCATGTGCATGGTTTAGGTGGTTCACTCAGGCCATGAGAAAGAATGGGTATTTTCAGAGTCATTCTGATCATACTTTATTTGTGAAGCGAAGGAACAGTAAAGTAACAGCCTTGATTATTTATGTAGATGATATGATAATAACAGGTGATGATTCAGATGAAATTATCAAGCTGGAGAAGAACCTTTCTGCGAAGTTTGAGATGAAAAATTTGGGTGACTTAAAGTATTTCCTTGGTGTTGAAGTTGCTCGTTCGTCTAGAGGTGATGTGACTGATAGGTGCTCTACTTCTGGATACTTTACGTTTGTTGGTGGAAATCTAGTTACTTGGAGAAGTAAAAAGCAAAATGTGGTGTCAAGGTCTTCAGCTGAGGCCGAATTCAGAGGTATGGCACATGGGATATGTGAAGTTCTTTGGTTACGGATGGATTTGGACTGTGGAGACTACTACCCAAACTTCACTCAAAGTTCGGTGTGGCAAGGGAAGGTGAAGGAGGTAGAAATCGACCGATCACTCAAGAACCTTTATGTGGTGCTTATGAGGCTAGGTTTCTTCGATGGGAGTCCCACTTTTAAGTCTCTTGGCAAGAAGGATGTGTGCTCCAAACAACATATTGAATTAGCAACAGAAGCAGCAAGGGAAGGAATCGTTTTATTGAAAAATGTCAATGAAACTTTGCCGTTGAAATCGAAGAAAATAAAAAAACTAGCCATCGTCGGGCCTCATGCCAATGCTACTGCAGCAATGATTGGAAACTATGCAGGTATTCCTTGCCAATTCACTTCACCTCTCGATGCATTTTCATCCTATGGAGAAGTTAAGTATGAAATGGGATGTGATGGAGTTGCATGTGCAAGTGAGAGTCTGATATTCCCTGCCATGAGGGCCGCAAAGCACGCAGATGCAACCATAATTTTTGCTGGGTTGGACTTATCAGTTGAGGCAGAGAGCTTGGATAGGGTGGATCTGCTTCTTCCTGGCTACCAAACCCAGTTTATCAATCAAGTTGCTCAAGTCTCCAAGGGTCCAGTGATTCTTGTAATCATGTCAGCTGGTGGTGTTGATATCAGTTTTGCCAAACAAAATGATAACATCAATGCAATATTGTGGGCTGGGTACCCGGGGGAGGAAGGAGGTCGTGCCATTGCAGATGTTGCTTTCGGACATTACAATCCTGGAGGAAGATTGCCTCTTCCATGGTATGAAGCTGGTTACGTGGATATGCTACCCATGACATCCATGCAATTGAGACCAATTGATAGCCTAGGCTACCCCGGTCGTACATACAAATTCTACAATGGCTCCACAGTTTGCCCATTTGGATACGGCCTTAGCTACACCCAGTTCAACTACACTCTGCGATCTGCAAAGAGATCTCTGGATATAAACTTGAGAAAGTCCCAACACTGCCGCGACATAGAGTACAAGGATCATGAATACAGGCCACCTTGCCCTGCAGTCCTTATTGACGACTTGCAACACAACCATGAATTTGGGTTCGGGGTTGAAGTTAAAAATGTGGGTAAGAGGGATGGAAGTGAAGTCATCATCGTTTACTCAAAACCACCAAATGGCATCGCCGCTACGCATGCCAAGCAAGTGATTGGGTTTAAAAGGGTTTTTGTGAAAGCTGGAAAAAGCGAAACTGTTAACTTCGTGTTCAATACCTGCAAGAGCTTGGGACTTGTCAACTACAATGCTTACAATCTTCTAACATCAGGCCGACACACAATTTCGATTGGCGATGATGTCGTCTCCTTCCCTATTCAAGTCAACATTAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

1386

Amino Acids

154.16

Weight (kDa)

8.46

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 66 - 227 3.3e-19 Glycosyl hydrolase family 3 N terminal domain
Pol_BBD PF22936 249 - 322 5.4e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 354 - 421 7.1e-09 GAG-pre-integrase domain
SH3_retrovirus PF25597 597 - 655 2.6e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 775 - 876 1.7e-23 Reverse transcriptase (RNA-dependent DNA polymerase)
Glyco_hydro_3_C PF01915 1006 - 1236 3.1e-45 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 1304 - 1373 1.8e-08 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 483, 1682
Acc36I ACCTGC 2 cut(s) 3122, 4046
Acc65I GGTACC 2 cut(s) 949, 3486
AccB1I GGYRCC 3 cut(s) 949, 2078, 3486
AccB7I CCANNNNNTGG 1 cut(s) 3941
AccBSI CCGCTC 1 cut(s) 869
AccI GTMKAC 4 cut(s) 408, 1129, 1502, 1550
AccII CGCG 2 cut(s) 575, 3783
AclI AACGTT 2 cut(s) 213, 2057
AclWI GGATC 8 cut(s) 224, 237, 509, 561, 1829, 1842, 3342, 3807
AcsI RAATTY 6 cut(s) 1524, 1709, 2578, 2747, 3659, 3860
AcuI CTGAAG 3 cut(s) 701, 995, 2718
AcyI GRCGYC 1 cut(s) 1656
AdeI CACNNNGTG 3 cut(s) 30, 263, 2375
AfiI CCNNNNNNNGG 6 cut(s) 239, 2355, 2899, 3087, 3496, 3941
AflIII ACRYGT 4 cut(s) 699, 1541, 1741, 1917
AjiI CACGTC 1 cut(s) 1410
AjnI CCWGG 3 cut(s) 446, 3346, 3544
AloI GAACNNNNNNTCC 2 cut(s) 1288, 1320
Alw21I GWGCWC 4 cut(s) 479, 1846, 2654, 2972
Alw26I GTCTC 9 cut(s) 885, 936, 1529, 2115, 2805, 2952, 3391, 3613, 4129
AlwI GGATC 8 cut(s) 224, 237, 509, 561, 1829, 1842, 3342, 3807
Ama87I CYCGRG 4 cut(s) 46, 1698, 1776, 3490
AoxI GGCC 9 cut(s) 664, 1337, 2400, 2742, 3089, 3255, 3697, 3812, 4090
ApeKI GCWGC 8 cut(s) 112, 381, 641, 644, 2169, 2236, 3002, 3110
ApoI RAATTY 6 cut(s) 1524, 1709, 2578, 2747, 3659, 3860
ArsI GACNNNNNNTTYG 2 cut(s) 1528, 1560
AseI ATTAAT 1 cut(s) 986
Asp700I GAANNNNTTC 2 cut(s) 1300, 1743
Asp718I GGTACC 2 cut(s) 949, 3486
AspA2I CCTAGG 1 cut(s) 3634
AspLEI GCGC 1 cut(s) 1315
AspS9I GGNCC 6 cut(s) 43, 604, 1337, 3089, 3255, 3395
AvaI CYCGRG 4 cut(s) 46, 1698, 1776, 3490
AvaII GGWCC 3 cut(s) 43, 604, 3395
AvrII CCTAGG 1 cut(s) 3634
AxyI CCTNAGG 1 cut(s) 2300
BaeGI GKGCMC 2 cut(s) 332, 1638
BaeI ACNNNNGTAYC 2 cut(s) 1956, 1989
BamHI GGATCC 2 cut(s) 229, 1834
BanI GGYRCC 3 cut(s) 949, 2078, 3486
BbsI GAAGAC 4 cut(s) 273, 707, 1227, 2724
Bbv12I GWGCWC 4 cut(s) 479, 1846, 2654, 2972
BbvCI CCTCAGC 1 cut(s) 2739
BbvI GCAGC 8 cut(s) 124, 368, 628, 631, 2156, 2248, 3014, 3122
BceAI ACGGC 4 cut(s) 1655, 2066, 3034, 3712
BciT130I CCWGG 3 cut(s) 448, 3348, 3546
BciVI GTATCC 3 cut(s) 1002, 2657, 3686
BclI TGATCA 3 cut(s) 427, 1516, 2437
BcoDI GTCTC 9 cut(s) 885, 936, 1529, 2115, 2805, 2952, 3391, 3613, 4129
BfmI CTRYAG 4 cut(s) 342, 1130, 3108, 3825
BfuAI ACCTGC 2 cut(s) 3122, 4046
BfuI GTATCC 3 cut(s) 1002, 2657, 3686
BglI GCCNNNNNGGC 1 cut(s) 3254
BglII AGATCT 2 cut(s) 359, 3743
BlnI CCTAGG 1 cut(s) 3634
BlpI GCTNAGC 1 cut(s) 620
BmcAI AGTACT 1 cut(s) 1354
Bme18I GGWCC 3 cut(s) 43, 604, 3395
BmeT110I CYCGRG 4 cut(s) 46, 1698, 1776, 3490
BmgBI CACGTC 1 cut(s) 1410
BmgT120I GGNCC 6 cut(s) 43, 604, 1337, 3089, 3255, 3395
BmrI ACTGGG 2 cut(s) 3355, 3706
BmuI ACTGGG 2 cut(s) 3355, 3706
BpiI GAAGAC 4 cut(s) 273, 707, 1227, 2724
BpmI CTGGAG 2 cut(s) 2564, 3567
Bpu10I CCTNAGC 2 cut(s) 2739, 3701
Bpu1102I GCTNAGC 1 cut(s) 620
BpuEI CTTGAG 4 cut(s) 939, 2870, 3366, 3781
BsaAI YACGTR 2 cut(s) 1118, 3586
BsaHI GRCGYC 1 cut(s) 1656
BsaI GGTCTC 2 cut(s) 2115, 3613
BsaXI ACNNNNNCTCC 8 cut(s) 1288, 1318, 1470, 1500, 1756, 1786, 3656, 3686
Bsc4I CCNNNNNNNGG 6 cut(s) 239, 2355, 2899, 3087, 3496, 3941
Bse1I ACTGG 6 cut(s) 123, 419, 1748, 3361, 3398, 3712
Bse21I CCTNAGG 1 cut(s) 2300
Bse3DI GCAATG 3 cut(s) 1613, 3120, 3513
BseBI CCWGG 3 cut(s) 448, 3348, 3546
BseLI CCNNNNNNNGG 6 cut(s) 239, 2355, 2899, 3087, 3496, 3941
BseMI GCAATG 3 cut(s) 1613, 3120, 3513
BseMII CTCAG 9 cut(s) 5, 634, 1239, 1485, 1566, 1938, 2291, 2411, 2730
BseNI ACTGG 6 cut(s) 123, 419, 1748, 3361, 3398, 3712
BseRI GAGGAG 2 cut(s) 558, 1764
BseSI GKGCMC 2 cut(s) 332, 1638
BseXI GCAGC 8 cut(s) 124, 368, 628, 631, 2156, 2248, 3014, 3122
BseYI CCCAGC 2 cut(s) 3290, 3482
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 2 cut(s) 575, 3783
Bsh1285I CGRYCG 2 cut(s) 2878, 3649
BshFI GGCC 9 cut(s) 666, 1339, 2402, 2744, 3091, 3257, 3699, 3814, 4092
BshNI GGYRCC 3 cut(s) 949, 2078, 3486
BsiEI CGRYCG 2 cut(s) 2878, 3649
BsiHKAI GWGCWC 4 cut(s) 479, 1846, 2654, 2972
BsiHKCI CYCGRG 4 cut(s) 46, 1698, 1776, 3490
BsiSI CCGG 7 cut(s) 47, 73, 189, 261, 1699, 3491, 3645
BslFI GGGAC 7 cut(s) 29, 63, 192, 2332, 2919, 3754, 4065
BslI CCNNNNNNNGG 6 cut(s) 239, 2355, 2899, 3087, 3496, 3941
BsmAI GTCTC 9 cut(s) 885, 936, 1529, 2115, 2805, 2952, 3391, 3613, 4129
BsmBI CGTCTC 1 cut(s) 4129
BsmFI GGGAC 7 cut(s) 29, 63, 192, 2332, 2919, 3754, 4065
BsnI GGCC 9 cut(s) 666, 1339, 2402, 2744, 3091, 3257, 3699, 3814, 4092
Bso31I GGTCTC 2 cut(s) 2115, 3613
BsoBI CYCGRG 4 cut(s) 46, 1698, 1776, 3490
Bsp1286I GDGCHC 6 cut(s) 332, 479, 1638, 1846, 2654, 2972
Bsp1407I TGTACA 2 cut(s) 171, 2044
Bsp1720I GCTNAGC 1 cut(s) 620
Bsp19I CCATGG 2 cut(s) 1929, 3566
BspANI GGCC 9 cut(s) 666, 1339, 2402, 2744, 3091, 3257, 3699, 3814, 4092
BspCNI CTCAG 9 cut(s) 6, 633, 1238, 1486, 1567, 1939, 2292, 2410, 2731
BspFNI CGCG 2 cut(s) 575, 3783
BspHI TCATGA 2 cut(s) 1531, 3802
BspMAI CTGCAG 2 cut(s) 3112, 3829
BspMI ACCTGC 2 cut(s) 3122, 4046
BspPI GGATC 8 cut(s) 224, 237, 509, 561, 1829, 1842, 3342, 3807
BspQI GCTCTTC 4 cut(s) 178, 238, 1087, 1851
BspT107I GGYRCC 3 cut(s) 949, 2078, 3486
BspTNI GGTCTC 2 cut(s) 2115, 3613
BsrBI CCGCTC 1 cut(s) 869
BsrDI GCAATG 3 cut(s) 1613, 3120, 3513
BsrGI TGTACA 2 cut(s) 171, 2044
BsrI ACTGG 6 cut(s) 123, 419, 1748, 3361, 3398, 3712
BssNAI GTATAC 1 cut(s) 1551
BssNI GRCGYC 1 cut(s) 1656
BssT1I CCWWGG 5 cut(s) 1929, 2602, 3390, 3566, 3634
Bst1107I GTATAC 1 cut(s) 1551
Bst2UI CCWGG 3 cut(s) 448, 3348, 3546
Bst6I CTCTTC 6 cut(s) 178, 238, 900, 1087, 1851, 3567
BstACI GRCGYC 1 cut(s) 1656
BstAPI GCANNNNNTGC 1 cut(s) 3524
BstAUI TGTACA 2 cut(s) 171, 2044
BstBAI YACGTR 2 cut(s) 1118, 3586
BstC8I GCNNGC 8 cut(s) 314, 664, 1007, 1167, 1691, 2222, 3267, 3960
BstDSI CCRYGG 4 cut(s) 24, 249, 1929, 3566
BstFNI CGCG 2 cut(s) 575, 3783
BstHHI GCGC 1 cut(s) 1315
BstMAI GTCTC 9 cut(s) 885, 936, 1529, 2115, 2805, 2952, 3391, 3613, 4129
BstMCI CGRYCG 2 cut(s) 2878, 3649
BstNI CCWGG 3 cut(s) 448, 3348, 3546
BstNSI RCATGY 6 cut(s) 1169, 1693, 1745, 1921, 3222, 3962
BstSFI CTRYAG 4 cut(s) 342, 1130, 3108, 3825
BstSLI GKGCMC 2 cut(s) 332, 1638
BstUI CGCG 2 cut(s) 575, 3783
BstV1I GCAGC 8 cut(s) 124, 368, 628, 631, 2156, 2248, 3014, 3122
BstV2I GAAGAC 4 cut(s) 273, 707, 1227, 2724
BstX2I RGATCY 6 cut(s) 229, 359, 553, 1834, 3334, 3743
BstXI CCANNNNNNTGG 2 cut(s) 2379, 3863
BstYI RGATCY 6 cut(s) 229, 359, 553, 1834, 3334, 3743
BstZ17I GTATAC 1 cut(s) 1551
Bsu36I CCTNAGG 1 cut(s) 2300
BsuI GTATCC 3 cut(s) 1002, 2657, 3686
BsuRI GGCC 9 cut(s) 666, 1339, 2402, 2744, 3091, 3257, 3699, 3814, 4092
BtgI CCRYGG 4 cut(s) 24, 249, 1929, 3566
BtgZI GCGATG 2 cut(s) 3931, 4128
BtrI CACGTC 1 cut(s) 1410
BtsI GCAGTG 2 cut(s) 472, 3775
BtsIMutI CAGTG 9 cut(s) 92, 472, 725, 882, 960, 1216, 2270, 3405, 3775
BveI ACCTGC 2 cut(s) 3122, 4046
Cac8I GCNNGC 8 cut(s) 314, 664, 1007, 1167, 1691, 2222, 3267, 3960
CciI TCATGA 2 cut(s) 1531, 3802
CfoI GCGC 1 cut(s) 1315
Cfr13I GGNCC 6 cut(s) 43, 604, 1337, 3089, 3255, 3395
Cfr9I CCCGGG 3 cut(s) 46, 1698, 3490
CseI GACGC 1 cut(s) 1664
DraI TTTAAA 1 cut(s) 3982
DraIII CACNNNGTG 3 cut(s) 30, 263, 2375
Eam1104I CTCTTC 6 cut(s) 178, 238, 900, 1087, 1851, 3567
EarI CTCTTC 6 cut(s) 178, 238, 900, 1087, 1851, 3567
Eco130I CCWWGG 5 cut(s) 1929, 2602, 3390, 3566, 3634
Eco31I GGTCTC 2 cut(s) 2115, 3613
Eco32I GATATC 2 cut(s) 2295, 3436
Eco47I GGWCC 3 cut(s) 43, 604, 3395
Eco57I CTGAAG 3 cut(s) 701, 995, 2718
Eco81I CCTNAGG 1 cut(s) 2300
Eco88I CYCGRG 4 cut(s) 46, 1698, 1776, 3490
EcoO109I RGGNCCY 1 cut(s) 1337
EcoRI GAATTC 2 cut(s) 1524, 2747
EcoRII CCWGG 3 cut(s) 446, 3346, 3544
EcoRV GATATC 2 cut(s) 2295, 3436
EcoT14I CCWWGG 5 cut(s) 1929, 2602, 3390, 3566, 3634
EcoT22I ATGCAT 4 cut(s) 1114, 1171, 1615, 3169
ErhI CCWWGG 5 cut(s) 1929, 2602, 3390, 3566, 3634
Esp3I CGTCTC 1 cut(s) 4129
FalI AAGNNNNNCTT 2 cut(s) 1512, 1544
FaqI GGGAC 7 cut(s) 29, 63, 192, 2332, 2919, 3754, 4065
FauI CCCGC 1 cut(s) 862
FauNDI CATATG 1 cut(s) 774
FbaI TGATCA 3 cut(s) 427, 1516, 2437
FblI GTMKAC 4 cut(s) 408, 1129, 1502, 1550
GlaI GCGC 1 cut(s) 1314
GsaI CCCAGC 2 cut(s) 3294, 3486
GsuI CTGGAG 2 cut(s) 2564, 3567
HaeIII GGCC 9 cut(s) 666, 1339, 2402, 2744, 3091, 3257, 3699, 3814, 4092
HapII CCGG 7 cut(s) 47, 73, 189, 261, 1699, 3491, 3645
HgaI GACGC 1 cut(s) 1664
HhaI GCGC 1 cut(s) 1315
Hin1I GRCGYC 1 cut(s) 1656
Hin6I GCGC 1 cut(s) 1313
HinP1I GCGC 1 cut(s) 1313
HincII GTYRAC 6 cut(s) 195, 1489, 1734, 4021, 4060, 4144
HindII GTYRAC 6 cut(s) 195, 1489, 1734, 4021, 4060, 4144
HindIII AAGCTT 2 cut(s) 84, 651
HpaI GTTAAC 2 cut(s) 1489, 4021
HpaII CCGG 7 cut(s) 47, 73, 189, 261, 1699, 3491, 3645
Hpy99I CGWCG 1 cut(s) 3089
Hsp92I GRCGYC 1 cut(s) 1656
HspAI GCGC 1 cut(s) 1313
KpnI GGTACC 2 cut(s) 953, 3490
Ksp22I TGATCA 3 cut(s) 427, 1516, 2437
KspAI GTTAAC 2 cut(s) 1489, 4021
LguI GCTCTTC 4 cut(s) 178, 238, 1087, 1851
LmnI GCTCC 3 cut(s) 866, 2975, 3677
Lsp1109I GCAGC 8 cut(s) 124, 368, 628, 631, 2156, 2248, 3014, 3122
MbiI CCGCTC 1 cut(s) 869
MfeI CAATTG 3 cut(s) 968, 3614, 3624
MflI RGATCY 6 cut(s) 229, 359, 553, 1834, 3334, 3743
MhlI GDGCHC 6 cut(s) 332, 479, 1638, 1846, 2654, 2972
MlyI GAGTC 7 cut(s) 214, 578, 1217, 1958, 2437, 2941, 3241
MmeI TCCRAC 4 cut(s) 725, 844, 2019, 3276
Mph1103I ATGCAT 4 cut(s) 1114, 1171, 1615, 3169
MroXI GAANNNNTTC 2 cut(s) 1300, 1743
MslI CAYNNNNRTG 9 cut(s) 441, 1326, 1403, 1434, 1934, 2377, 3099, 3270, 3855
MspA1I CMGCKG 3 cut(s) 2224, 2738, 3422
MspI CCGG 7 cut(s) 47, 73, 189, 261, 1699, 3491, 3645
MunI CAATTG 3 cut(s) 968, 3614, 3624
MvaI CCWGG 3 cut(s) 448, 3348, 3546
MvnI CGCG 2 cut(s) 575, 3783
NcoI CCATGG 2 cut(s) 1929, 3566
NdeI CATATG 1 cut(s) 774
NmuCI GTSAC 6 cut(s) 581, 958, 1936, 2367, 2585, 2638
NsiI ATGCAT 4 cut(s) 1114, 1171, 1615, 3169
NspI RCATGY 6 cut(s) 1169, 1693, 1745, 1921, 3222, 3962
PaeI GCATGC 3 cut(s) 1169, 1693, 3962
PaeR7I CTCGAG 1 cut(s) 1776
PagI TCATGA 2 cut(s) 1531, 3802
PciI ACATGT 2 cut(s) 1741, 1917
PciSI GCTCTTC 4 cut(s) 178, 238, 1087, 1851
PdmI GAANNNNTTC 2 cut(s) 1300, 1743
PfeI GAWTC 7 cut(s) 755, 1365, 1439, 1816, 2522, 3015, 3403
PflMI CCANNNNNTGG 1 cut(s) 3941
PfoI TCCNGGA 1 cut(s) 3544
PleI GAGTC 7 cut(s) 213, 578, 1217, 1957, 2436, 2940, 3240
PpsI GAGTC 7 cut(s) 213, 578, 1217, 1957, 2436, 2940, 3240
Ppu21I YACGTR 2 cut(s) 1118, 3586
PscI ACATGT 2 cut(s) 1741, 1917
PshBI ATTAAT 1 cut(s) 986
PsiI TTATAA 2 cut(s) 483, 1682
Psp1406I AACGTT 2 cut(s) 213, 2057
Psp6I CCWGG 3 cut(s) 446, 3346, 3544
PspFI CCCAGC 2 cut(s) 3290, 3482
PspGI CCWGG 3 cut(s) 446, 3346, 3544
PspPI GGNCC 6 cut(s) 43, 604, 1337, 3089, 3255, 3395
PspXI VCTCGAGB 1 cut(s) 1776
PstI CTGCAG 2 cut(s) 3112, 3829
PsuI RGATCY 6 cut(s) 229, 359, 553, 1834, 3334, 3743
PvuII CAGCTG 3 cut(s) 2224, 2738, 3422
RseI CAYNNNNRTG 9 cut(s) 441, 1326, 1403, 1434, 1934, 2377, 3099, 3270, 3855
SapI GCTCTTC 4 cut(s) 178, 238, 1087, 1851
Sau96I GGNCC 6 cut(s) 43, 604, 1337, 3089, 3255, 3395
ScaI AGTACT 1 cut(s) 1354
SchI GAGTC 7 cut(s) 214, 578, 1217, 1958, 2437, 2941, 3241
SduI GDGCHC 6 cut(s) 332, 479, 1638, 1846, 2654, 2972
SfcI CTRYAG 4 cut(s) 342, 1130, 3108, 3825
Sfr274I CTCGAG 1 cut(s) 1776
SinI GGWCC 3 cut(s) 43, 604, 3395
SlaI CTCGAG 1 cut(s) 1776
SmaI CCCGGG 3 cut(s) 48, 1700, 3492
SmiMI CAYNNNNRTG 9 cut(s) 441, 1326, 1403, 1434, 1934, 2377, 3099, 3270, 3855
SmlI CTYRAG 5 cut(s) 954, 1776, 2885, 3381, 3760
SmoI CTYRAG 5 cut(s) 954, 1776, 2885, 3381, 3760
SphI GCATGC 3 cut(s) 1169, 1693, 3962
SspI AATATT 2 cut(s) 1958, 3474
StyI CCWWGG 5 cut(s) 1929, 2602, 3390, 3566, 3634
TaqII GACCGA 2 cut(s) 2097, 2892
TatI WGTACW 6 cut(s) 171, 1318, 1352, 2044, 2206, 3792
TauI GCSGC 5 cut(s) 398, 726, 3260, 3783, 3953
TfiI GAWTC 7 cut(s) 755, 1365, 1439, 1816, 2522, 3015, 3403
TscAI CASTG 9 cut(s) 99, 472, 725, 882, 967, 1216, 2270, 3405, 3782
TseFI GTSAC 6 cut(s) 581, 958, 1936, 2367, 2585, 2638
TseI GCWGC 8 cut(s) 112, 381, 641, 644, 2169, 2236, 3002, 3110
Tsp45I GTSAC 6 cut(s) 581, 958, 1936, 2367, 2585, 2638
TspGWI ACGGA 8 cut(s) 266, 576, 912, 1435, 1502, 1751, 2328, 2806
TspMI CCCGGG 3 cut(s) 46, 1698, 3490
TspRI CASTG 9 cut(s) 99, 472, 725, 882, 967, 1216, 2270, 3405, 3782
Van91I CCANNNNNTGG 1 cut(s) 3941
VpaK11BI GGWCC 3 cut(s) 43, 604, 3395
VspI ATTAAT 1 cut(s) 986
XapI RAATTY 6 cut(s) 1524, 1709, 2578, 2747, 3659, 3860
XbaI TCTAGA 2 cut(s) 356, 2627
XceI RCATGY 6 cut(s) 1169, 1693, 1745, 1921, 3222, 3962
XhoI CTCGAG 1 cut(s) 1776
XmaI CCCGGG 3 cut(s) 46, 1698, 3490
XmaJI CCTAGG 1 cut(s) 3634
XmiI GTMKAC 4 cut(s) 408, 1129, 1502, 1550
XmnI GAANNNNTTC 2 cut(s) 1300, 1743
ZrmI AGTACT 1 cut(s) 1354
Zsp2I ATGCAT 4 cut(s) 1114, 1171, 1615, 3169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.