MD05G1289800.v1.1

UDP-N-acetylglucosamine transferase subunit ALG14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006662
Physical Location & Seq
Forward (+)
42186705 .. 42188017
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1289800.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGGCTGCGCCGTCGCAATCACTCTTTTAATCGCCGCCGCAACGCTGATCATCACTCGCTTCCTCTACGTCCTGCACCGAACCGGCAAACCCAGCAGCTCGAAAGGCTCAGCTCCCCAACCCCTCAGCACCCTAATCGTATTGGGCTCCGGAGGCCACACCGCAGAGATGATCAACCTGTTGTCCGTCCTTCAAAAAGACAGATTTGCCCCCAGGTTCTACATCGCCGCCGCCACTGATAACATGAGTCTCCAGAAAGCTCGACTGCTCGAAGAGAACGCCGATGAGAGTTCGAGCTCGCAGTTCATGCAGATCTACCGGAGCAGGGAAGTGGGTCAGTCTTATTTCACCTCTGTTTTGACTGCTTTGATTGCTTTGGCTCATGGGCTCTGGCTAATGATCAGAATCCGACCCCAAGTGATTCTGTGCAATGGGCCTGGGACTTGTGTTCCACTTTGTGTAATTGCATTCTTATTCAAGGTTGTGGGGATTAGATGGTCGTCTGTGTTTTATGTTGAGAGTATAGCAAGAGTGCAGAGGCTCTCCCTGAGCGGCTTGCTTCTTTACAAGTTACGGATAGCGGATCAGTTCTTTGTCCAATGGCCTCAGTTACAGAAGAAGTATCCCAGAGCTCACTATGTCGGTTGTCTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00510 ko00513 ko01100 map00510 map00513 map01100
Pfam Domains
Protein Families
Alg14

Protein Analysis

219

Amino Acids

24.23

Weight (kDa)

9.84

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alg14 PF08660 45 - 217 1.5e-68 Oligosaccharide biosynthesis protein Alg14 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 7 cut(s) 36, 39, 162, 228, 231, 552, 581
AclWI GGATC 1 cut(s) 591
AdeI CACNNNGTG 1 cut(s) 458
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 2 cut(s) 194, 478
AjnI CCWGG 2 cut(s) 212, 436
AluBI AGCT 5 cut(s) 99, 113, 260, 297, 632
AluI AGCT 5 cut(s) 99, 113, 260, 297, 632
Alw21I GWGCWC 2 cut(s) 299, 634
Alw26I GTCTC 2 cut(s) 254, 653
AlwI GGATC 1 cut(s) 591
Aor13HI TCCGGA 1 cut(s) 149
AoxI GGCC 3 cut(s) 154, 434, 602
ApeKI GCWGC 2 cut(s) 6, 96
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 1 cut(s) 340
BanII GRGCYC 4 cut(s) 149, 299, 390, 634
Bbv12I GWGCWC 2 cut(s) 299, 634
BbvCI CCTCAGC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 108
BccI CCATC 1 cut(s) 489
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 214, 438
BciVI GTATCC 1 cut(s) 633
BclI TGATCA 3 cut(s) 48, 171, 399
BcoDI GTCTC 2 cut(s) 254, 653
BfuI GTATCC 1 cut(s) 633
BglII AGATCT 1 cut(s) 312
BisI GCNGC 7 cut(s) 7, 36, 39, 97, 228, 231, 553
BlpI GCTNAGC 1 cut(s) 109
BlsI GCNGC 7 cut(s) 8, 37, 40, 98, 229, 232, 554
Bme1390I CCNGG 2 cut(s) 214, 438
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 2 cut(s) 214, 438
BpmI CTGGAG 1 cut(s) 236
Bpu10I CCTNAGC 2 cut(s) 125, 548
Bpu1102I GCTNAGC 1 cut(s) 109
BsaBI GATNNNNATC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 212, 437
BsaWI WCCGGW 2 cut(s) 149, 318
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse118I RCCGGY 1 cut(s) 83
Bse3DI GCAATG 1 cut(s) 436
Bse8I GATNNNNATC 1 cut(s) 404
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 2 cut(s) 214, 438
BseDI CCNNGG 2 cut(s) 212, 437
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 4 cut(s) 123, 139, 539, 620
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 92
BsgI GTGCAG 2 cut(s) 59, 554
BshFI GGCC 3 cut(s) 156, 436, 604
BsiHKAI GWGCWC 2 cut(s) 299, 634
BsiSI CCGG 3 cut(s) 84, 150, 319
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 325
BsmAI GTCTC 2 cut(s) 254, 653
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 3 cut(s) 156, 436, 604
Bsp1286I GDGCHC 4 cut(s) 149, 299, 390, 634
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 5 cut(s) 48, 171, 312, 399, 583
Bsp1720I GCTNAGC 1 cut(s) 109
BspACI CCGC 7 cut(s) 36, 39, 162, 228, 231, 552, 581
BspANI GGCC 3 cut(s) 156, 436, 604
BspCNI CTCAG 4 cut(s) 122, 138, 540, 619
BspEI TCCGGA 1 cut(s) 149
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 1 cut(s) 591
BsrBI CCGCTC 1 cut(s) 552
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 83
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 2 cut(s) 212, 437
BssMI GATC 5 cut(s) 48, 171, 312, 399, 583
Bst2UI CCWGG 2 cut(s) 214, 438
Bst6I CTCTTC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 307
BstC8I GCNNGC 2 cut(s) 299, 557
BstDEI CTNAG 4 cut(s) 109, 125, 548, 606
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 5 cut(s) 51, 174, 315, 402, 586
BstMAI GTCTC 2 cut(s) 254, 653
BstMBI GATC 5 cut(s) 48, 171, 312, 399, 583
BstMWI GCNNNNNNNGC 5 cut(s) 93, 105, 153, 307, 371
BstNI CCWGG 2 cut(s) 214, 438
BstSCI CCNGG 2 cut(s) 212, 436
BstV1I GCAGC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 3 cut(s) 156, 436, 604
BtgZI GCGATG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 299, 557
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 434
CviAII CATG 4 cut(s) 244, 307, 383, 652
DdeI CTNAG 4 cut(s) 109, 125, 548, 606
DpnI GATC 5 cut(s) 50, 173, 314, 401, 585
DpnII GATC 5 cut(s) 48, 171, 312, 399, 583
DraIII CACNNNGTG 1 cut(s) 458
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 2 cut(s) 297, 632
Eco24I GRGCYC 4 cut(s) 149, 299, 390, 634
Eco53kI GAGCTC 2 cut(s) 297, 632
EcoICRI GAGCTC 2 cut(s) 297, 632
EcoRII CCWGG 2 cut(s) 212, 436
EcoT38I GRGCYC 4 cut(s) 149, 299, 390, 634
FaeI CATG 4 cut(s) 247, 310, 386, 655
FaiI YATR 7 cut(s) 245, 308, 384, 513, 524, 639, 653
FaqI GGGAC 1 cut(s) 454
FatI CATG 4 cut(s) 243, 306, 382, 651
FbaI TGATCA 3 cut(s) 48, 171, 399
Fnu4HI GCNGC 7 cut(s) 7, 36, 39, 97, 228, 231, 553
FriOI GRGCYC 4 cut(s) 149, 299, 390, 634
Fsp4HI GCNGC 7 cut(s) 7, 36, 39, 97, 228, 231, 553
GlaI GCGC 1 cut(s) 10
GluI GCNGC 7 cut(s) 7, 36, 39, 97, 228, 231, 553
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 236
HaeIII GGCC 3 cut(s) 156, 436, 604
HapII CCGG 3 cut(s) 84, 150, 319
HhaI GCGC 1 cut(s) 11
Hin1II CATG 4 cut(s) 247, 310, 386, 655
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 3 cut(s) 247, 405, 421
HpaII CCGG 3 cut(s) 84, 150, 319
HphI GGTGA 1 cut(s) 340
Hpy188I TCNGA 2 cut(s) 404, 410
Hpy188III TCNNGA 2 cut(s) 150, 253
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 5 cut(s) 76, 310, 429, 467, 535
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 105, 153, 307, 371
HpyF3I CTNAG 4 cut(s) 109, 125, 548, 606
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 4 cut(s) 247, 310, 386, 655
HspAI GCGC 1 cut(s) 9
Kpn2I TCCGGA 1 cut(s) 149
Ksp22I TGATCA 3 cut(s) 48, 171, 399
Kzo9I GATC 5 cut(s) 48, 171, 312, 399, 583
LmnI GCTCC 3 cut(s) 118, 152, 321
Lsp1109I GCAGC 1 cut(s) 108
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 570, 609
MalI GATC 5 cut(s) 50, 173, 314, 401, 585
MbiI CCGCTC 1 cut(s) 552
MboI GATC 5 cut(s) 48, 171, 312, 399, 583
MboII GAAGA 2 cut(s) 284, 628
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 4 cut(s) 149, 299, 390, 634
MluCI AATT 1 cut(s) 462
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 6 cut(s) 74, 134, 146, 361, 531, 615
MroI TCCGGA 1 cut(s) 149
MseI TTAA 1 cut(s) 29
MspI CCGG 3 cut(s) 84, 150, 319
MspR9I CCNGG 2 cut(s) 214, 438
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 2 cut(s) 214, 438
MwoI GCNNNNNNNGC 5 cut(s) 93, 105, 153, 307, 371
NdeII GATC 5 cut(s) 48, 171, 312, 399, 583
NlaIII CATG 4 cut(s) 247, 310, 386, 655
NlaIV GGNNCC 1 cut(s) 148
PctI GAATGC 1 cut(s) 467
PfeI GAWTC 2 cut(s) 405, 421
PkrI GCNGC 7 cut(s) 8, 37, 40, 98, 229, 232, 554
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Psp124BI GAGCTC 2 cut(s) 299, 634
Psp6I CCWGG 2 cut(s) 212, 436
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 212, 436
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 434
PsuI RGATCY 1 cut(s) 312
SacI GAGCTC 2 cut(s) 299, 634
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 7 cut(s) 7, 36, 39, 97, 228, 231, 553
Sau3AI GATC 5 cut(s) 48, 171, 312, 399, 583
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 2 cut(s) 214, 438
SduI GDGCHC 4 cut(s) 149, 299, 390, 634
Sse9I AATT 1 cut(s) 462
SsiI CCGC 7 cut(s) 36, 39, 162, 228, 231, 552, 581
SstI GAGCTC 2 cut(s) 299, 634
StyD4I CCNGG 2 cut(s) 212, 436
TaiI ACGT 1 cut(s) 72
TaqI TCGA 4 cut(s) 101, 262, 270, 293
TasI AATT 1 cut(s) 462
TauI GCSGC 5 cut(s) 38, 41, 230, 233, 555
TfiI GAWTC 2 cut(s) 405, 421
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 6, 96
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 2 cut(s) 175, 589
TspRI CASTG 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.