MD05G1295500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014687
Physical Location & Seq
Reverse (-)
43013161 .. 43013614
454 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1295500.v1.1.491

Sequence Viewer

Length: 336 bp
ATGCCTTCTGGTACTGAGTCTGAGCTCTCTTCTCCTTCTTCTTCGTCCACACCTGGGTTGCCTGACACGTGTCCTCTTTCCGCTTGTCCAACTGTGCAGTTGGTCCCAAAATCTACCTCTGATCGACTTCTGCAGAAGTTCTTCGATGCCACGGAGTTCGACTTTGATTACGAGCAGAGCGGATTGTGGTCTCCTCTGGTCCGGCGTACGGTGTTCCTGAGCTCTCAAGGAAAAATCGTCACTGACCAAGAAATGCTTGCCAGACTTTCAACCCTAATGGATGCTCCTTCTGCAAGACACGATAAAGCTTGTTTTGGGGGATGCTGGTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.07

Weight (kDa)

4.75

Isoelectric Point (pI)

78.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014687)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07320
malus_domestica MD00G1009400.v1.1 MD05G1295500.v1.1 MD10G1275600.v1.1
prunus_persica Prupe.4G066600_v2.0.a1
pyrus_communis pycom05g27450 pycom10g23020
rosa_chinensis RchiOBHm_Chr5g0011631
rosa_laevigata RLG00000031850
rosa_multiflora Rmu_sc0009001.1_g000016
rosa_roxburghii Rroxscaffold_1G00064870
rosa_rugosa Rorug04G0458200
rosa_samantha Rh5AG089900 Rh5BG085500 Rh5CG099400 Rh5DG085300
rosa_wichuraiana Rw5G007820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 180
AciI CCGC 2 cut(s) 81, 180
AcvI CACGTG 1 cut(s) 69
AfaI GTAC 2 cut(s) 13, 208
AfiI CCNNNNNNNGG 2 cut(s) 54, 208
AflIII ACRYGT 2 cut(s) 66, 68
AgsI TTSAA 1 cut(s) 270
AjnI CCWGG 1 cut(s) 52
AluBI AGCT 3 cut(s) 25, 222, 308
AluI AGCT 3 cut(s) 25, 222, 308
Alw21I GWGCWC 2 cut(s) 27, 224
Alw26I GTCTC 1 cut(s) 195
Asp700I GAANNNNTTC 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 103, 199
AvaII GGWCC 2 cut(s) 103, 199
BanII GRGCYC 2 cut(s) 27, 224
BbrPI CACGTG 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 27, 224
BciT130I CCWGG 1 cut(s) 54
BcoDI GTCTC 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 131
Bme1390I CCNGG 1 cut(s) 54
Bme18I GGWCC 2 cut(s) 103, 199
BmgT120I GGNCC 2 cut(s) 103, 199
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 3 cut(s) 136, 271, 311
BoxI GACNNNNGTC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 53, 150
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 208
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 53, 150
BseGI GGATG 2 cut(s) 286, 326
BseLI CCNNNNNNNGG 2 cut(s) 54, 208
BseMII CTCAG 3 cut(s) 6, 12, 209
BseRI GAGGAG 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 116
BsiHKAI GWGCWC 2 cut(s) 27, 224
BsiSI CCGG 1 cut(s) 202
BsiWI CGTACG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 2 cut(s) 54, 208
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 89
Bso31I GGTCTC 1 cut(s) 195
Bsp1286I GDGCHC 2 cut(s) 27, 224
Bsp143I GATC 1 cut(s) 121
BspACI CCGC 2 cut(s) 81, 180
BspCNI CTCAG 3 cut(s) 7, 13, 210
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 135
BspTNI GGTCTC 1 cut(s) 195
BsrBI CCGCTC 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 53, 150
BssMI GATC 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 2 cut(s) 94, 211
Bst6I CTCTTC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 3 cut(s) 15, 21, 218
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 286, 326
BstKTI GATC 1 cut(s) 124
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNI CCWGG 1 cut(s) 54
BstPAI GACNNNNGTC 1 cut(s) 69
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 286, 326
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 103, 199
Csp6I GTAC 2 cut(s) 12, 207
CviJI RGCY 3 cut(s) 25, 222, 308
CviKI_1 RGCY 3 cut(s) 25, 222, 308
CviQI GTAC 2 cut(s) 12, 207
DdeI CTNAG 3 cut(s) 15, 21, 218
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Ecl136II GAGCTC 2 cut(s) 25, 222
Eco24I GRGCYC 2 cut(s) 27, 224
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 103, 199
Eco53kI GAGCTC 2 cut(s) 25, 222
Eco72I CACGTG 1 cut(s) 69
EcoICRI GAGCTC 2 cut(s) 25, 222
EcoRII CCWGG 1 cut(s) 52
EcoT38I GRGCYC 2 cut(s) 27, 224
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 89
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 27, 224
HapII CCGG 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 1 cut(s) 17
HpaII CCGG 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 2 cut(s) 22, 121
Hpy188III TCNNGA 2 cut(s) 217, 333
Hpy8I GTNNAC 1 cut(s) 48
HpyAV CCTTC 3 cut(s) 15, 45, 297
HpyCH4III ACNGT 2 cut(s) 94, 211
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 3 cut(s) 97, 133, 293
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 3 cut(s) 15, 21, 218
HpySE526I ACGT 1 cut(s) 68
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 8 cut(s) 39, 66, 75, 182, 215, 230, 274, 310
LweI GCATC 3 cut(s) 136, 271, 311
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 1 cut(s) 238
MalI GATC 1 cut(s) 123
MbiI CCGCTC 1 cut(s) 180
MboI GATC 1 cut(s) 121
MboII GAAGA 4 cut(s) 21, 30, 33, 133
MhlI GDGCHC 2 cut(s) 27, 224
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 3 cut(s) 84, 127, 204
MroXI GAANNNNTTC 1 cut(s) 140
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 1 cut(s) 121
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 140
Pfl23II CGTACG 1 cut(s) 206
PleI GAGTC 1 cut(s) 25
PmaCI CACGTG 1 cut(s) 69
PmlI CACGTG 1 cut(s) 69
PpsI GAGTC 1 cut(s) 25
Ppu21I YACGTR 1 cut(s) 69
PshAI GACNNNNGTC 1 cut(s) 69
Psp124BI GAGCTC 2 cut(s) 27, 224
Psp6I CCWGG 1 cut(s) 52
PspCI CACGTG 1 cut(s) 69
PspGI CCWGG 1 cut(s) 52
PspLI CGTACG 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 103, 199
PstI CTGCAG 1 cut(s) 135
RsaI GTAC 2 cut(s) 13, 208
RsaNI GTAC 2 cut(s) 12, 207
SacI GAGCTC 2 cut(s) 27, 224
Sau3AI GATC 1 cut(s) 121
Sau96I GGNCC 2 cut(s) 103, 199
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 2 cut(s) 27, 224
SetI ASST 6 cut(s) 27, 55, 71, 119, 224, 310
SfaNI GCATC 3 cut(s) 136, 271, 311
SfcI CTRYAG 1 cut(s) 131
SinI GGWCC 2 cut(s) 103, 199
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SsiI CCGC 2 cut(s) 81, 180
SstI GAGCTC 2 cut(s) 27, 224
StyD4I CCNGG 1 cut(s) 52
TaaI ACNGT 2 cut(s) 94, 211
TaiI ACGT 1 cut(s) 71
TaqI TCGA 3 cut(s) 124, 144, 159
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 103, 199
XmnI GAANNNNTTC 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.