Rorug04G0458200

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000908
Physical Location & Seq
Reverse (-)
61110942 .. 61114566
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0458200.1

Sequence Viewer

Length: 912 bp
ATGGCGGATGTTACTAGTACGAAGCAGAGCGAGGCAGCCGAGAAAGTCGACTACCGCAACCTGCCCTGCCCCATACCCTATGAAGAAATCCACCGCGAAGCCATGATGTCTTTAAAGCCAGACCTTTTTGAGGGGATGCGCTTTGACTTCACCAAAGGACTAAATCACAAGTTCTTCCTTAGCCACAGTGTATTCATGGGACCTACAACCATTCCTTCTCAAACTCCTGAAACCATAAAAATCCCTACTGCTAACTATGAGTTTGGTGCAAATTTCATAGACCACCCAAAGTGCCTGCTTTCTGGGAGAGTAATGACAGATGGAAGACTCAGTGCAAGAGTGAAGTATGACTTGAGTGACAATTTCACTTTGAAGGCTAATGCTCAGCTTACAAATGAGCCACATATGTCTCATGGCATTGTCAATGTTGATTACAAGGGTTCAGACCACAGAGCCCAAGTTCAGATGGGAAATGGGACCCTAATTGGGGCGAATTACGTCCAGTCTGTGACTCCACATTTCTCTTTGGGTGGCGAAGTATTTTGGGCTGGTCAGCATCGAAAATCGGGTATCAGTTATGGGGCACGATTCAACACTGACAAGATGGTTGCTACGGGGCAGGTTGCTAGCACTGGAATGGTTGCATTGAACTATGTCCAGCGAGTGTCTGAGAAGGTTTCACTAGCATCAGACTTCATGTACAATTACATGTCAAGCGATGTCACAGCAAGTTTTGGCTATGATTACATTTTCAGACAGAGTCGTATTAGAGGAAAGATTGATACCAATGGCTGCACAGCTGCTTTTCTGGAAGAGAGATTAACTATGGGTCTTAATTTTATTCTTTCTGCAGAGATAGATCACATGAAGAAAGACTACAAATTTGGGTTTGGGATGACAGTTGGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

303

Amino Acids

33.84

Weight (kDa)

6.8

Isoelectric Point (pI)

20.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Porin_3 PF01459 23 - 296 3.4e-61 Eukaryotic porin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 69, 610
AccI GTMKAC 1 cut(s) 48
AccII CGCG 1 cut(s) 96
AciI CCGC 3 cut(s) 5, 55, 94
AcsI RAATTY 2 cut(s) 271, 881
AfaI GTAC 2 cut(s) 19, 701
AfiI CCNNNNNNNGG 3 cut(s) 130, 486, 487
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 3 cut(s) 373, 592, 649
AhlI ACTAGT 1 cut(s) 14
AluBI AGCT 2 cut(s) 388, 800
AluI AGCT 2 cut(s) 388, 800
Alw26I GTCTC 1 cut(s) 414
ApeKI GCWGC 3 cut(s) 35, 792, 800
ApoI RAATTY 2 cut(s) 271, 881
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 200, 477
AsuHPI GGTGA 1 cut(s) 142
AsuNHI GCTAGC 1 cut(s) 626
AvaII GGWCC 2 cut(s) 200, 477
BaeGI GKGCMC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 457
BarI GAAGNNNNNNTAC 2 cut(s) 860, 892
BbsI GAAGAC 1 cut(s) 331
BbvI GCAGC 3 cut(s) 47, 779, 787
BccI CCATC 3 cut(s) 314, 460, 598
BcoDI GTCTC 1 cut(s) 414
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 3 cut(s) 15, 627, 683
BfmI CTRYAG 1 cut(s) 849
BfuAI ACCTGC 2 cut(s) 69, 610
BisI GCNGC 3 cut(s) 36, 793, 801
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 3 cut(s) 37, 794, 802
Bme18I GGWCC 2 cut(s) 200, 477
BmgT120I GGNCC 2 cut(s) 200, 477
BmiI GGNNCC 3 cut(s) 201, 478, 479
BmsI GCATC 3 cut(s) 126, 565, 695
BmtI GCTAGC 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 179
Bpu1102I GCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 373
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 486, 487
Bse1I ACTGG 2 cut(s) 502, 637
BseGI GGATG 3 cut(s) 13, 141, 900
BseLI CCNNNNNNNGG 3 cut(s) 130, 486, 487
BseMII CTCAG 3 cut(s) 343, 398, 660
BseNI ACTGG 2 cut(s) 502, 637
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 3 cut(s) 47, 779, 787
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 96
BslFI GGGAC 2 cut(s) 213, 490
BslI CCNNNNNNNGG 3 cut(s) 130, 486, 487
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 2 cut(s) 213, 490
Bsp1286I GDGCHC 2 cut(s) 457, 586
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 1 cut(s) 859
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 3 cut(s) 5, 55, 94
BspCNI CTCAG 3 cut(s) 342, 397, 661
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 201, 478, 479
BspMAI CTGCAG 1 cut(s) 853
BspMI ACCTGC 2 cut(s) 69, 610
BspOI GCTAGC 1 cut(s) 630
BsrGI TGTACA 1 cut(s) 699
BsrI ACTGG 2 cut(s) 502, 637
BssMI GATC 1 cut(s) 859
Bst4CI ACNGT 2 cut(s) 188, 901
Bst6I CTCTTC 1 cut(s) 807
BstAUI TGTACA 1 cut(s) 699
BstC8I GCNNGC 2 cut(s) 296, 628
BstDEI CTNAG 4 cut(s) 179, 329, 384, 669
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 3 cut(s) 13, 141, 900
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 1 cut(s) 862
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 1 cut(s) 859
BstNSI RCATGY 1 cut(s) 712
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 47, 779, 787
BstV2I GAAGAC 1 cut(s) 331
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 3 cut(s) 13, 141, 900
BtsIMutI CAGTG 4 cut(s) 193, 337, 594, 630
BveI ACCTGC 2 cut(s) 69, 610
Cac8I GCNNGC 2 cut(s) 296, 628
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 2 cut(s) 200, 477
Csp6I GTAC 2 cut(s) 18, 700
CviAII CATG 6 cut(s) 103, 196, 413, 697, 709, 865
CviQI GTAC 2 cut(s) 18, 700
DdeI CTNAG 4 cut(s) 179, 329, 384, 669
DpnI GATC 1 cut(s) 861
DpnII GATC 1 cut(s) 859
DraI TTTAAA 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 200, 477
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 2 cut(s) 200, 477
EcoT38I GRGCYC 1 cut(s) 457
FaeI CATG 6 cut(s) 106, 199, 416, 700, 712, 868
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 2 cut(s) 213, 490
FatI CATG 6 cut(s) 102, 195, 412, 696, 708, 864
FauNDI CATATG 1 cut(s) 405
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 36, 793, 801
FokI GGATG 3 cut(s) 20, 148, 907
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 3 cut(s) 36, 793, 801
FspBI CTAG 3 cut(s) 15, 627, 683
GlaI GCGC 1 cut(s) 140
GluI GCNGC 3 cut(s) 36, 793, 801
HhaI GCGC 1 cut(s) 141
Hin1II CATG 6 cut(s) 106, 199, 416, 700, 712, 868
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 4 cut(s) 327, 511, 588, 760
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 5 cut(s) 445, 465, 670, 691, 755
Hpy188III TCNNGA 2 cut(s) 227, 809
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 225, 367, 667
HpyCH4III ACNGT 2 cut(s) 188, 901
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 5 cut(s) 269, 335, 644, 795, 851
HpyF3I CTNAG 4 cut(s) 179, 329, 384, 669
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 6 cut(s) 106, 199, 416, 700, 712, 868
HspAI GCGC 1 cut(s) 139
KflI GGGWCCC 1 cut(s) 477
Kzo9I GATC 1 cut(s) 859
Lsp1109I GCAGC 3 cut(s) 47, 779, 787
LweI GCATC 3 cut(s) 126, 565, 695
MaeI CTAG 3 cut(s) 15, 627, 683
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 4 cut(s) 10, 356, 508, 721
MalI GATC 1 cut(s) 861
MboI GATC 1 cut(s) 859
MboII GAAGA 5 cut(s) 95, 166, 336, 824, 880
MhlI GDGCHC 2 cut(s) 457, 586
MluCI AATT 7 cut(s) 271, 361, 483, 493, 703, 835, 881
MlyI GAGTC 3 cut(s) 321, 505, 769
MmeI TCCRAC 1 cut(s) 883
MnlI CCTC 3 cut(s) 25, 124, 764
MseI TTAA 3 cut(s) 113, 821, 834
MslI CAYNNNNRTG 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 800
MvnI CGCG 1 cut(s) 96
NdeI CATATG 1 cut(s) 405
NdeII GATC 1 cut(s) 859
NheI GCTAGC 1 cut(s) 626
NlaIII CATG 6 cut(s) 106, 199, 416, 700, 712, 868
NlaIV GGNNCC 3 cut(s) 201, 478, 479
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 3 cut(s) 356, 508, 721
NspI RCATGY 1 cut(s) 712
PciI ACATGT 1 cut(s) 708
PfeI GAWTC 1 cut(s) 588
PflFI GACNNNGTC 1 cut(s) 759
PkrI GCNGC 3 cut(s) 37, 794, 802
PleI GAGTC 3 cut(s) 321, 505, 768
PpsI GAGTC 3 cut(s) 321, 505, 768
PpuMI RGGWCCY 2 cut(s) 200, 477
PscI ACATGT 1 cut(s) 708
Psp5II RGGWCCY 2 cut(s) 200, 477
PspN4I GGNNCC 3 cut(s) 201, 478, 479
PspPI GGNCC 2 cut(s) 200, 477
PspPPI RGGWCCY 2 cut(s) 200, 477
PstI CTGCAG 1 cut(s) 853
PsyI GACNNNGTC 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 2 cut(s) 19, 701
RsaNI GTAC 2 cut(s) 18, 700
RseI CAYNNNNRTG 1 cut(s) 635
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 3 cut(s) 113, 821, 834
SatI GCNGC 3 cut(s) 36, 793, 801
Sau3AI GATC 1 cut(s) 859
Sau96I GGNCC 2 cut(s) 200, 477
SchI GAGTC 3 cut(s) 321, 505, 769
SduI GDGCHC 2 cut(s) 457, 586
SetI ASST 8 cut(s) 63, 126, 205, 390, 501, 624, 678, 802
SfaNI GCATC 3 cut(s) 126, 565, 695
SfcI CTRYAG 1 cut(s) 849
SinI GGWCC 2 cut(s) 200, 477
SmiMI CAYNNNNRTG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 7 cut(s) 271, 361, 483, 493, 703, 835, 881
SsiI CCGC 3 cut(s) 5, 55, 94
SspMI CTAG 3 cut(s) 15, 627, 683
TaaI ACNGT 2 cut(s) 188, 901
TaiI ACGT 1 cut(s) 501
TaqI TCGA 2 cut(s) 48, 559
TasI AATT 7 cut(s) 271, 361, 483, 493, 703, 835, 881
TatI WGTACW 1 cut(s) 699
TfiI GAWTC 1 cut(s) 588
Tru1I TTAA 3 cut(s) 113, 821, 834
Tru9I TTAA 3 cut(s) 113, 821, 834
TscAI CASTG 4 cut(s) 193, 337, 601, 637
TseFI GTSAC 3 cut(s) 356, 508, 721
TseI GCWGC 3 cut(s) 35, 792, 800
Tsp45I GTSAC 3 cut(s) 356, 508, 721
TspDTI ATGAA 5 cut(s) 96, 184, 265, 685, 881
TspRI CASTG 4 cut(s) 193, 337, 601, 637
Tth111I GACNNNGTC 1 cut(s) 759
VpaK11BI GGWCC 2 cut(s) 200, 477
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 2 cut(s) 271, 881
XceI RCATGY 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 3 cut(s) 15, 627, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.