RchiOBHm_Chr5g0061601

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000908
Physical Location & Seq
Forward (+)
67107968 .. 67112040
4073 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33795

Sequence Viewer

Length: 585 bp
ATGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAATGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATGCCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTGCGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCCTGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

194

Amino Acids

21.55

Weight (kDa)

4.91

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 39 - 138 1.9e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 477
AciI CCGC 1 cut(s) 199
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 3 cut(s) 62, 270, 387
AfaI GTAC 2 cut(s) 126, 576
AfiI CCNNNNNNNGG 1 cut(s) 502
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 6 cut(s) 23, 81, 205, 235, 241, 532
AjnI CCWGG 2 cut(s) 116, 163
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 2 cut(s) 154, 339
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 152, 290
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 3 cut(s) 62, 270, 387
BbvI GCAGC 1 cut(s) 385
BccI CCATC 2 cut(s) 23, 530
BciT130I CCWGG 2 cut(s) 118, 165
BcoDI GTCTC 2 cut(s) 154, 339
BfmI CTRYAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 325
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
Bme1390I CCNGG 2 cut(s) 118, 165
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 118, 165
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BplI GAGNNNNNCTC 2 cut(s) 312, 344
BpmI CTGGAG 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 116, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse1I ACTGG 3 cut(s) 74, 427, 578
BseBI CCWGG 2 cut(s) 118, 165
BseDI CCNNGG 2 cut(s) 116, 340
BseGI GGATG 1 cut(s) 541
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMII CTCAG 3 cut(s) 147, 297, 489
BseNI ACTGG 3 cut(s) 74, 427, 578
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 1 cut(s) 385
BshFI GGCC 2 cut(s) 154, 292
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 2 cut(s) 154, 339
BsnI GGCC 2 cut(s) 154, 292
Bso31I GGTCTC 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 3 cut(s) 103, 172, 325
BspACI CCGC 1 cut(s) 199
BspANI GGCC 2 cut(s) 154, 292
BspCNI CTCAG 3 cut(s) 148, 298, 490
BspLI GGNNCC 1 cut(s) 312
BspMAI CTGCAG 1 cut(s) 142
BspPI GGATC 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 124
BsrI ACTGG 3 cut(s) 74, 427, 578
BssECI CCNNGG 2 cut(s) 116, 340
BssMI GATC 3 cut(s) 103, 172, 325
BssT1I CCWWGG 1 cut(s) 340
Bst2UI CCWGG 2 cut(s) 118, 165
Bst4CI ACNGT 1 cut(s) 475
Bst6I CTCTTC 1 cut(s) 369
BstAUI TGTACA 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 2 cut(s) 315, 464
BstDEI CTNAG 5 cut(s) 156, 176, 306, 498, 559
BstF5I GGATG 1 cut(s) 541
BstKTI GATC 3 cut(s) 106, 175, 328
BstMAI GTCTC 2 cut(s) 154, 339
BstMBI GATC 3 cut(s) 103, 172, 325
BstNI CCWGG 2 cut(s) 118, 165
BstSCI CCNGG 2 cut(s) 116, 163
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 385
BstX2I RGATCY 2 cut(s) 103, 325
BstYI RGATCY 2 cut(s) 103, 325
BsuRI GGCC 2 cut(s) 154, 292
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 1 cut(s) 541
Cac8I GCNNGC 2 cut(s) 315, 464
Csp6I GTAC 2 cut(s) 125, 575
CviJI RGCY 7 cut(s) 70, 135, 154, 292, 313, 411, 466
CviKI_1 RGCY 7 cut(s) 70, 135, 154, 292, 313, 411, 466
CviQI GTAC 2 cut(s) 125, 575
DdeI CTNAG 5 cut(s) 156, 176, 306, 498, 559
DpnI GATC 3 cut(s) 105, 174, 327
DpnII GATC 3 cut(s) 103, 172, 325
DraI TTTAAA 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco130I CCWWGG 1 cut(s) 340
Eco147I AGGCCT 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 154
Eco32I GATATC 1 cut(s) 366
EcoRI GAATTC 1 cut(s) 270
EcoRII CCWGG 2 cut(s) 116, 163
EcoRV GATATC 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 340
ErhI CCWWGG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 154, 292
Hpy166II GTNNAC 4 cut(s) 127, 182, 478, 517
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 3 cut(s) 226, 260, 369
Hpy8I GTNNAC 4 cut(s) 127, 182, 478, 517
HpyAV CCTTC 4 cut(s) 50, 235, 496, 526
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 3 cut(s) 140, 299, 401
HpyF3I CTNAG 5 cut(s) 156, 176, 306, 498, 559
HpySE526I ACGT 1 cut(s) 129
Kzo9I GATC 3 cut(s) 103, 172, 325
LmnI GCTCC 3 cut(s) 75, 310, 320
Lsp1109I GCAGC 1 cut(s) 385
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 1 cut(s) 145
MalI GATC 3 cut(s) 105, 174, 327
MboI GATC 3 cut(s) 103, 172, 325
MboII GAAGA 1 cut(s) 386
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 2 cut(s) 103, 325
MluCI AATT 8 cut(s) 62, 212, 236, 255, 270, 282, 387, 540
MnlI CCTC 6 cut(s) 198, 241, 282, 301, 370, 430
MseI TTAA 5 cut(s) 66, 254, 302, 441, 522
MspR9I CCNGG 2 cut(s) 118, 165
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 2 cut(s) 118, 165
NdeII GATC 3 cut(s) 103, 172, 325
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 1 cut(s) 130
PceI AGGCCT 1 cut(s) 292
PkrI GCNGC 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 130
Psp6I CCWGG 2 cut(s) 116, 163
PspGI CCWGG 2 cut(s) 116, 163
PspN4I GGNNCC 1 cut(s) 312
PstI CTGCAG 1 cut(s) 142
PsuI RGATCY 2 cut(s) 103, 325
RsaI GTAC 2 cut(s) 126, 576
RsaNI GTAC 2 cut(s) 125, 575
SaqAI TTAA 5 cut(s) 66, 254, 302, 441, 522
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 3 cut(s) 103, 172, 325
ScrFI CCNGG 2 cut(s) 118, 165
SetI ASST 7 cut(s) 72, 122, 132, 166, 190, 381, 441
SfcI CTRYAG 1 cut(s) 138
Sse9I AATT 8 cut(s) 62, 212, 236, 255, 270, 282, 387, 540
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 1 cut(s) 199
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 116, 163
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 1 cut(s) 132
TasI AATT 8 cut(s) 62, 212, 236, 255, 270, 282, 387, 540
TatI WGTACW 1 cut(s) 124
Tru1I TTAA 5 cut(s) 66, 254, 302, 441, 522
Tru9I TTAA 5 cut(s) 66, 254, 302, 441, 522
TseI GCWGC 1 cut(s) 398
XapI RAATTY 3 cut(s) 62, 270, 387
XmiI GTMKAC 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.