Prupe.4G066900_v2.0.a1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000908
Physical Location & Seq
Reverse (-)
3229716 .. 3234195
4480 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G066900.2

Sequence Viewer

Length: 1812 bp
ATGGAATTTGGTGTCATTAAGCTATCTTCGCCTCTGTCTTCTTCAGCTCTCAATCTCCAGGATTATTCATCACCTCTTGGTAGGAATCATTTTAAAAAGTCTTTACATCCTCAGTTGCTGTCCTCTCCACTTCCTAAACCCTTTCTTCAACTTAATGACAGACCACACAGCTTTCCCTACCAACAAAACTCTGATCATCTCTCCCCAAGAACGTATCGAAGATCCATATCAGCAGAAGCTGGAAAGCAGAGTTGGGACTTGGGTAGATTTTTGAAAACAATATTTTTCTTCAACGAACCTCCCACTCCTGCTAAGTTCTTTGAGTTTGTAAGTGAGAAACTCTCCAGCCCATCACCTAGTAAACCAGAAAAGAAAATGGAAACTTCTGGCATTGTCCTAGTGGCTGGAGCAACTGGTGGTGTAGGTCGAAGGGTGGTCGACGTCTTAAGGAAGAAAGGGCTTCCGGTCCGAGTATTGGTTAGAAATGAAGAGAAAGCAAGGAAAATGTTAGGCCCGGATGTTGACTTGATTGTTGGAGACATTACTAAGGAGAGTACTCTGGTTCCCGAGTACTTCAAAGGAGTGAGGAGAGTGATTAATGCTGTTTCTGTCATTGTTGGTCCAAAGGAAGGTGACACTCCTGACAGGCAAAAATACAGCCAGGGAATCAAGTTCTTTGAACCAGAGATCAAGGGGGATTCGCCTGAAAAGGTGGAGTACATTGGAATAAGAAATTTGATCAATGCTGTAAAAGGAAGCGTTGGACTTCAAGATGGAAAACTACTATTTGGATTTGAAGATAATAATTATAGGGAATTGGCTTGGGGTGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCAGATTGATCCAAACGGTGGTGAAAATGGTGGACCAACTGGAATTTTTAAAGGGGTTGTTTCCACTGCAAACAATGGTGGTTTTACCAGTATCAGAACAAAGAATCTATCAACAGCTGAGGATCTCTCTGCATATGATGGTTTGGAGCTGCGCCTTAAAGGTGATGGTCGTCGCTATAAACTAATTGTTCGCACAAGTCGTGATTGGGATACTGTTGGTTACACATCAGGCTTTGACACTGTTGGGGACCAGTGGCAGACAGTACGTGTGCCATTTTCTTCTTTGAAGCCGATATTTCGAGCAAGAACCGTATCGGATGCACCACCTTTTGACCCCAGCAATATTGTATCATTGCAGCTCATGTTTAGCAAGTTTGAATATGATGGGAAACTGAATCCTACTTTTGTGGAAGGGGCATTTAAGCTCCCATTATCAAGCATACGAGCATATCTAAAAGAGCCTATTACTCCCAGGTTCGTACACGTCGGTTCTGCAGGAGTTACCCGACCTGACAGGCCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTTCGCTTAAACAAGGAATTGGATTTTATCCTGACATTCAAGTTGAAGGGGGAGGATTTAATACGGGAAAGTGGAATACCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCCGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAGGAGGTTGCTCAGATTTGTGTTGCTGCGTTGGAAAGCCACTATGCATCTGGCAAGACATTTGAGGTTAAAAGTGTTGTGCCATTTAGTGAGCCATTTACAGTGGACCCTCAAAATCCACCCCCCGAGAAGGACTACAATGTTTACTTTAAAACTTTGAAAGATGGAATTACAGGAAAGGAAATCCTCGAACAAGATCCTGTACCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

604

Amino Acids

66.63

Weight (kDa)

8.44

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 890
AccI GTMKAC 1 cut(s) 438
AclWI GGATC 4 cut(s) 216, 875, 1002, 1790
AcsI RAATTY 3 cut(s) 5, 733, 915
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 7 cut(s) 556, 572, 719, 868, 1137, 1353, 1803
AfiI CCNNNNNNNGG 4 cut(s) 475, 629, 890, 1729
AflII CTTAAG 1 cut(s) 445
AflIII ACRYGT 2 cut(s) 1138, 1354
AjiI CACGTC 1 cut(s) 1357
AjnI CCWGG 4 cut(s) 57, 660, 1343, 1390
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AluBI AGCT 8 cut(s) 22, 47, 171, 239, 989, 1021, 1231, 1297
AluI AGCT 8 cut(s) 22, 47, 171, 239, 989, 1021, 1231, 1297
Alw26I GTCTC 2 cut(s) 531, 1566
AlwI GGATC 4 cut(s) 216, 875, 1002, 1790
AlwNI CAGNNNCTG 2 cut(s) 118, 239
Ama87I CYCGRG 2 cut(s) 566, 1724
AoxI GGCC 3 cut(s) 511, 1388, 1517
ApeKI GCWGC 3 cut(s) 1021, 1228, 1625
ApoI RAATTY 3 cut(s) 5, 733, 915
AseI ATTAAT 1 cut(s) 597
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 6 cut(s) 466, 512, 620, 905, 1120, 1705
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 5 cut(s) 63, 345, 644, 905, 1046
AvaI CYCGRG 2 cut(s) 566, 1724
AvaII GGWCC 5 cut(s) 466, 620, 905, 1120, 1705
BbsI GAAGAC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 990
BbvI GCAGC 3 cut(s) 1008, 1240, 1612
BccI CCATC 7 cut(s) 358, 767, 841, 1004, 1031, 1250, 1757
BciT130I CCWGG 4 cut(s) 59, 662, 1345, 1392
BciVI GTATCC 1 cut(s) 1075
BclI TGATCA 2 cut(s) 193, 738
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 531, 1566
BfaI CTAG 2 cut(s) 357, 398
BfmI CTRYAG 1 cut(s) 1365
BfrI CTTAAG 1 cut(s) 445
BfuI GTATCC 1 cut(s) 1075
BglII AGATCT 1 cut(s) 1552
BisI GCNGC 3 cut(s) 1022, 1229, 1626
BlsI GCNGC 3 cut(s) 1023, 1230, 1627
BmcAI AGTACT 2 cut(s) 556, 572
Bme1390I CCNGG 5 cut(s) 59, 515, 662, 1345, 1392
Bme18I GGWCC 5 cut(s) 466, 620, 905, 1120, 1705
BmeT110I CYCGRG 2 cut(s) 566, 1724
BmgBI CACGTC 1 cut(s) 1357
BmgT120I GGNCC 6 cut(s) 466, 512, 620, 905, 1120, 1705
BmiI GGNNCC 4 cut(s) 564, 1121, 1539, 1707
BmrFI CCNGG 5 cut(s) 59, 515, 662, 1345, 1392
BmsI GCATC 2 cut(s) 1180, 1655
BpiI GAAGAC 1 cut(s) 30
BplI GAGNNNNNCTC 6 cut(s) 326, 358, 983, 1015, 1539, 1571
BpmI CTGGAG 3 cut(s) 41, 328, 426
Bpu10I CCTNAGC 1 cut(s) 990
BpuMI CCSGG 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 1139
BsaBI GATNNNNATC 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 661, 1343, 1567
BsaWI WCCGGW 1 cut(s) 463
Bsc4I CCNNNNNNNGG 4 cut(s) 475, 629, 890, 1729
Bse1I ACTGG 5 cut(s) 418, 916, 960, 1123, 1805
Bse3DI GCAATG 1 cut(s) 1223
Bse8I GATNNNNATC 1 cut(s) 226
BseBI CCWGG 4 cut(s) 59, 662, 1345, 1392
BseDI CCNNGG 3 cut(s) 661, 1343, 1567
BseGI GGATG 3 cut(s) 106, 523, 1195
BseJI GATNNNNATC 1 cut(s) 226
BseLI CCNNNNNNNGG 4 cut(s) 475, 629, 890, 1729
BseMI GCAATG 1 cut(s) 1223
BseMII CTCAG 4 cut(s) 125, 981, 1524, 1625
BseNI ACTGG 5 cut(s) 418, 916, 960, 1123, 1805
BseRI GAGGAG 3 cut(s) 601, 1550, 1616
BseXI GCAGC 3 cut(s) 1008, 1240, 1612
BseYI CCCAGC 1 cut(s) 1208
BshFI GGCC 3 cut(s) 513, 1390, 1519
BsiHKCI CYCGRG 2 cut(s) 566, 1724
BsiSI CCGG 3 cut(s) 464, 515, 1545
BslFI GGGAC 2 cut(s) 269, 1133
BslI CCNNNNNNNGG 4 cut(s) 475, 629, 890, 1729
BsmAI GTCTC 2 cut(s) 531, 1566
BsmFI GGGAC 2 cut(s) 269, 1133
BsnI GGCC 3 cut(s) 513, 1390, 1519
BsoBI CYCGRG 2 cut(s) 566, 1724
Bsp143I GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
BspANI GGCC 3 cut(s) 513, 1390, 1519
BspCNI CTCAG 4 cut(s) 124, 982, 1525, 1624
BspLI GGNNCC 4 cut(s) 564, 1121, 1539, 1707
BspMAI CTGCAG 1 cut(s) 1369
BspPI GGATC 4 cut(s) 216, 875, 1002, 1790
BspTI CTTAAG 1 cut(s) 445
BsrDI GCAATG 1 cut(s) 1223
BsrI ACTGG 5 cut(s) 418, 916, 960, 1123, 1805
BssECI CCNNGG 3 cut(s) 661, 1343, 1567
BssMI GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
BssNI GRCGYC 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 1567
Bst2UI CCWGG 4 cut(s) 59, 662, 1345, 1392
Bst4CI ACNGT 6 cut(s) 890, 1087, 1114, 1135, 1183, 1702
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 1 cut(s) 441
BstAFI CTTAAG 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 1139
BstC8I GCNNGC 1 cut(s) 1542
BstDEI CTNAG 7 cut(s) 111, 312, 546, 990, 1403, 1533, 1611
BstF5I GGATG 3 cut(s) 106, 523, 1195
BstHHI GCGC 1 cut(s) 1026
BstKTI GATC 9 cut(s) 196, 224, 690, 741, 883, 997, 1402, 1555, 1798
BstMAI GTCTC 2 cut(s) 531, 1566
BstMBI GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
BstMWI GCNNNNNNNGC 2 cut(s) 28, 827
BstNI CCWGG 4 cut(s) 59, 662, 1345, 1392
BstSCI CCNGG 5 cut(s) 57, 513, 660, 1343, 1390
BstSFI CTRYAG 1 cut(s) 1365
BstV1I GCAGC 3 cut(s) 1008, 1240, 1612
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 4 cut(s) 221, 994, 1552, 1795
BstYI RGATCY 4 cut(s) 221, 994, 1552, 1795
BsuI GTATCC 1 cut(s) 1075
BsuRI GGCC 3 cut(s) 513, 1390, 1519
BtrI CACGTC 1 cut(s) 1357
BtsCI GGATG 3 cut(s) 106, 523, 1195
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 5 cut(s) 936, 1110, 1130, 1707, 1812
Cac8I GCNNGC 1 cut(s) 1542
CaiI CAGNNNCTG 2 cut(s) 118, 239
CfoI GCGC 1 cut(s) 1026
Cfr13I GGNCC 6 cut(s) 466, 512, 620, 905, 1120, 1705
CpoI CGGWCCG 1 cut(s) 466
Csp6I GTAC 7 cut(s) 555, 571, 718, 867, 1136, 1352, 1802
CspI CGGWCCG 1 cut(s) 466
CviAII CATG 1 cut(s) 1234
CviQI GTAC 7 cut(s) 555, 571, 718, 867, 1136, 1352, 1802
DdeI CTNAG 7 cut(s) 111, 312, 546, 990, 1403, 1533, 1611
DpnI GATC 9 cut(s) 195, 223, 689, 740, 882, 996, 1401, 1554, 1797
DpnII GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
DraI TTTAAA 3 cut(s) 94, 922, 1750
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 1567
Eco147I AGGCCT 2 cut(s) 1390, 1519
Eco32I GATATC 1 cut(s) 1593
Eco47I GGWCC 5 cut(s) 466, 620, 905, 1120, 1705
Eco57I CTGAAG 1 cut(s) 27
Eco88I CYCGRG 2 cut(s) 566, 1724
EcoRII CCWGG 4 cut(s) 57, 660, 1343, 1390
EcoRV GATATC 1 cut(s) 1593
EcoT14I CCWWGG 1 cut(s) 1567
EcoT22I ATGCAT 1 cut(s) 1648
ErhI CCWWGG 1 cut(s) 1567
FaeI CATG 1 cut(s) 1237
FaqI GGGAC 2 cut(s) 269, 1133
FatI CATG 1 cut(s) 1233
FauNDI CATATG 1 cut(s) 1006
FbaI TGATCA 2 cut(s) 193, 738
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 1022, 1229, 1626
FokI GGATG 3 cut(s) 93, 530, 1202
Fsp4HI GCNGC 3 cut(s) 1022, 1229, 1626
FspBI CTAG 2 cut(s) 357, 398
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 3 cut(s) 1022, 1229, 1626
GsaI CCCAGC 1 cut(s) 1212
GsuI CTGGAG 3 cut(s) 41, 328, 426
HaeIII GGCC 3 cut(s) 513, 1390, 1519
HapII CCGG 3 cut(s) 464, 515, 1545
HhaI GCGC 1 cut(s) 1026
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 1 cut(s) 1237
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HincII GTYRAC 2 cut(s) 439, 523
HindII GTYRAC 2 cut(s) 439, 523
HinfI GANTC 5 cut(s) 85, 666, 698, 976, 1267
HpaII CCGG 3 cut(s) 464, 515, 1545
HphI GGTGA 5 cut(s) 63, 345, 644, 905, 1046
Hpy166II GTNNAC 8 cut(s) 362, 439, 523, 905, 1354, 1409, 1705, 1744
Hpy188I TCNGA 6 cut(s) 193, 470, 876, 968, 1189, 1614
Hpy188III TCNNGA 6 cut(s) 566, 641, 770, 1073, 1453, 1596
Hpy8I GTNNAC 8 cut(s) 362, 439, 523, 905, 1354, 1409, 1705, 1744
Hpy99I CGWCG 3 cut(s) 443, 1047, 1361
HpyAV CCTTC 5 cut(s) 423, 623, 1277, 1462, 1723
HpyCH4III ACNGT 6 cut(s) 890, 1087, 1114, 1135, 1183, 1702
HpyCH4IV ACGT 4 cut(s) 212, 441, 1138, 1356
HpyCH4V TGCA 7 cut(s) 941, 1004, 1193, 1228, 1367, 1526, 1646
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 827
HpyF3I CTNAG 7 cut(s) 111, 312, 546, 990, 1403, 1533, 1611
HpySE526I ACGT 4 cut(s) 212, 441, 1138, 1356
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 1 cut(s) 1237
HspAI GCGC 1 cut(s) 1024
Ksp22I TGATCA 2 cut(s) 193, 738
Kzo9I GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
LmnI GCTCC 5 cut(s) 407, 1018, 1302, 1537, 1547
Lsp1109I GCAGC 3 cut(s) 1008, 1240, 1612
LweI GCATC 2 cut(s) 1180, 1655
MaeI CTAG 2 cut(s) 357, 398
MaeII ACGT 4 cut(s) 212, 441, 1138, 1356
MaeIII GTNAC 3 cut(s) 632, 1091, 1372
MalI GATC 9 cut(s) 195, 223, 689, 740, 882, 996, 1401, 1554, 1797
MboI GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 4 cut(s) 221, 994, 1552, 1795
MluCI AATT 9 cut(s) 5, 733, 805, 815, 915, 1056, 1439, 1509, 1767
MmeI TCCRAC 3 cut(s) 514, 742, 1611
Mph1103I ATGCAT 1 cut(s) 1648
MspA1I CMGCKG 1 cut(s) 989
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 3 cut(s) 464, 515, 1545
MspR9I CCNGG 5 cut(s) 59, 515, 662, 1345, 1392
MunI CAATTG 1 cut(s) 1509
MvaI CCWGG 4 cut(s) 59, 662, 1345, 1392
MwoI GCNNNNNNNGC 2 cut(s) 28, 827
NciI CCSGG 1 cut(s) 515
NdeI CATATG 1 cut(s) 1006
NdeII GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
NlaIII CATG 1 cut(s) 1237
NlaIV GGNNCC 4 cut(s) 564, 1121, 1539, 1707
NmuCI GTSAC 1 cut(s) 632
NsiI ATGCAT 1 cut(s) 1648
PceI AGGCCT 2 cut(s) 1390, 1519
PcsI WCGNNNNNNNCGW 1 cut(s) 1069
PfeI GAWTC 5 cut(s) 85, 666, 698, 976, 1267
PflMI CCANNNNNTGG 1 cut(s) 890
PfoI TCCNGGA 1 cut(s) 57
PkrI GCNGC 3 cut(s) 1023, 1230, 1627
Ppu21I YACGTR 1 cut(s) 1139
PshBI ATTAAT 1 cut(s) 597
Psp6I CCWGG 4 cut(s) 57, 660, 1343, 1390
PspFI CCCAGC 1 cut(s) 1208
PspGI CCWGG 4 cut(s) 57, 660, 1343, 1390
PspN4I GGNNCC 4 cut(s) 564, 1121, 1539, 1707
PspPI GGNCC 6 cut(s) 466, 512, 620, 905, 1120, 1705
PstI CTGCAG 1 cut(s) 1369
PstNI CAGNNNCTG 2 cut(s) 118, 239
PsuI RGATCY 4 cut(s) 221, 994, 1552, 1795
PvuII CAGCTG 1 cut(s) 989
RsaI GTAC 7 cut(s) 556, 572, 719, 868, 1137, 1353, 1803
RsaNI GTAC 7 cut(s) 555, 571, 718, 867, 1136, 1352, 1802
Rsr2I CGGWCCG 1 cut(s) 466
RsrII CGGWCCG 1 cut(s) 466
SalI GTCGAC 1 cut(s) 437
SatI GCNGC 3 cut(s) 1022, 1229, 1626
Sau3AI GATC 9 cut(s) 193, 221, 687, 738, 880, 994, 1399, 1552, 1795
Sau96I GGNCC 6 cut(s) 466, 512, 620, 905, 1120, 1705
ScaI AGTACT 2 cut(s) 556, 572
ScrFI CCNGG 5 cut(s) 59, 515, 662, 1345, 1392
SfaNI GCATC 2 cut(s) 1180, 1655
SfcI CTRYAG 1 cut(s) 1365
SinI GGWCC 5 cut(s) 466, 620, 905, 1120, 1705
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
Sse9I AATT 9 cut(s) 5, 733, 805, 815, 915, 1056, 1439, 1509, 1767
SseBI AGGCCT 2 cut(s) 1390, 1519
SspI AATATT 2 cut(s) 282, 1216
SspMI CTAG 2 cut(s) 357, 398
StuI AGGCCT 2 cut(s) 1390, 1519
StyD4I CCNGG 5 cut(s) 57, 513, 660, 1343, 1390
StyI CCWWGG 1 cut(s) 1567
TaaI ACNGT 6 cut(s) 890, 1087, 1114, 1135, 1183, 1702
TaiI ACGT 4 cut(s) 215, 444, 1141, 1359
TaqI TCGA 5 cut(s) 217, 427, 438, 1171, 1788
TasI AATT 9 cut(s) 5, 733, 805, 815, 915, 1056, 1439, 1509, 1767
TatI WGTACW 4 cut(s) 554, 570, 717, 866
TfiI GAWTC 5 cut(s) 85, 666, 698, 976, 1267
TscAI CASTG 5 cut(s) 943, 1117, 1130, 1707, 1812
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 3 cut(s) 1021, 1228, 1625
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 2 cut(s) 57, 501
TspRI CASTG 5 cut(s) 943, 1117, 1130, 1707, 1812
Van91I CCANNNNNTGG 1 cut(s) 890
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 5 cut(s) 466, 620, 905, 1120, 1705
VspI ATTAAT 1 cut(s) 597
XapI RAATTY 3 cut(s) 5, 733, 915
XcmI CCANNNNNNNNNTGG 1 cut(s) 1646
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 2 cut(s) 357, 398
ZraI GACGTC 1 cut(s) 442
ZrmI AGTACT 2 cut(s) 556, 572
Zsp2I ATGCAT 1 cut(s) 1648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.