RchiOBHm_Chr2g0109871

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000908
Physical Location & Seq
Forward (+)
21209686 .. 21211471
1786 bp
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UTR
Exon/CDS
Intron
PRQ48364

Sequence Viewer

Length: 423 bp
ATGGCTGAACATAATCAGCTTGGTTCTGCTTCTTTCTTTACATCATTTCCTTTGTTCACATGTAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTACACTCTTGACTTGTATCCCGTACTTTGTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

140

Amino Acids

15.36

Weight (kDa)

5.15

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 22 - 120 1e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 145
AcsI RAATTY 2 cut(s) 216, 333
AfaI GTAC 2 cut(s) 72, 410
AflIII ACRYGT 2 cut(s) 59, 73
AgsI TTSAA 3 cut(s) 151, 181, 187
AjnI CCWGG 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 100, 285
AoxI GGCC 2 cut(s) 98, 236
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 2 cut(s) 216, 333
BbvI GCAGC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 111
BciVI GTATCC 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 100, 285
BfmI CTRYAG 1 cut(s) 84
BfuI GTATCC 1 cut(s) 413
BglII AGATCT 1 cut(s) 271
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 111
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 286
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 93, 243
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 269
BseXI GCAGC 1 cut(s) 331
BshFI GGCC 2 cut(s) 100, 238
BsmAI GTCTC 2 cut(s) 100, 285
BsnI GGCC 2 cut(s) 100, 238
Bso31I GGTCTC 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 2 cut(s) 118, 271
BspACI CCGC 1 cut(s) 145
BspANI GGCC 2 cut(s) 100, 238
BspCNI CTCAG 2 cut(s) 94, 244
BspLI GGNNCC 1 cut(s) 258
BspMAI CTGCAG 1 cut(s) 88
BspTNI GGTCTC 1 cut(s) 100
BsrGI TGTACA 1 cut(s) 70
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 2 cut(s) 118, 271
BssT1I CCWWGG 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 111
Bst6I CTCTTC 1 cut(s) 315
BstAUI TGTACA 1 cut(s) 70
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 3 cut(s) 102, 122, 252
BstKTI GATC 2 cut(s) 121, 274
BstMAI GTCTC 2 cut(s) 100, 285
BstMBI GATC 2 cut(s) 118, 271
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 2 cut(s) 100, 238
Cac8I GCNNGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 71, 409
CviAII CATG 1 cut(s) 60
CviJI RGCY 7 cut(s) 5, 19, 81, 100, 238, 259, 357
CviKI_1 RGCY 7 cut(s) 5, 19, 81, 100, 238, 259, 357
CviQI GTAC 2 cut(s) 71, 409
DdeI CTNAG 3 cut(s) 102, 122, 252
DpnI GATC 2 cut(s) 120, 273
DpnII GATC 2 cut(s) 118, 271
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 286
Eco147I AGGCCT 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 100
Eco32I GATATC 1 cut(s) 312
EcoRI GAATTC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 1 cut(s) 63
FaiI YATR 6 cut(s) 12, 61, 223, 225, 299, 361
FatI CATG 1 cut(s) 59
Fnu4HI GCNGC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 345
GluI GCNGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 2 cut(s) 100, 238
Hin1II CATG 1 cut(s) 63
Hpy166II GTNNAC 3 cut(s) 57, 73, 128
Hpy188III TCNNGA 4 cut(s) 172, 206, 315, 394
Hpy8I GTNNAC 3 cut(s) 57, 73, 128
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 86, 245, 347
HpyF3I CTNAG 3 cut(s) 102, 122, 252
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 1 cut(s) 63
Kzo9I GATC 2 cut(s) 118, 271
LmnI GCTCC 2 cut(s) 256, 266
LpnPI CCDG 9 cut(s) 72, 96, 114, 123, 150, 185, 249, 273, 354
Lsp1109I GCAGC 1 cut(s) 331
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 2 cut(s) 91, 385
MalI GATC 2 cut(s) 120, 273
MboI GATC 2 cut(s) 118, 271
MboII GAAGA 1 cut(s) 332
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 271
MluCI AATT 6 cut(s) 158, 182, 201, 216, 228, 333
MnlI CCTC 6 cut(s) 144, 187, 228, 247, 316, 376
MseI TTAA 3 cut(s) 200, 248, 421
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 111
NdeII GATC 2 cut(s) 118, 271
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 1 cut(s) 258
NmeAIII GCCGAG 1 cut(s) 76
NspI RCATGY 1 cut(s) 63
PceI AGGCCT 1 cut(s) 238
PciI ACATGT 1 cut(s) 59
PkrI GCNGC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 76
PscI ACATGT 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 258
PstI CTGCAG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 271
RsaI GTAC 2 cut(s) 72, 410
RsaNI GTAC 2 cut(s) 71, 409
SaqAI TTAA 3 cut(s) 200, 248, 421
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 2 cut(s) 118, 271
ScrFI CCNGG 1 cut(s) 111
SetI ASST 7 cut(s) 21, 68, 78, 112, 136, 327, 387
SfcI CTRYAG 1 cut(s) 84
Sse9I AATT 6 cut(s) 158, 182, 201, 216, 228, 333
SseBI AGGCCT 1 cut(s) 238
SsiI CCGC 1 cut(s) 145
StuI AGGCCT 1 cut(s) 238
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 286
TaiI ACGT 1 cut(s) 78
TasI AATT 6 cut(s) 158, 182, 201, 216, 228, 333
TatI WGTACW 1 cut(s) 70
Tru1I TTAA 3 cut(s) 200, 248, 421
Tru9I TTAA 3 cut(s) 200, 248, 421
TseI GCWGC 1 cut(s) 344
XapI RAATTY 2 cut(s) 216, 333
XceI RCATGY 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.