RchiOBHm_Chr5g0011651

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000908
Physical Location & Seq
Reverse (-)
7705784 .. 7709338
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29228

Sequence Viewer

Length: 1242 bp
ATGGAACTTGGTGTCATTAAGCTCTCATCTTCCTCAGCTCTCAATCTTCAGGATTTTTCAGCACCTGTTGGTAGGAAACTTATTAACAAACCTTCACTTCCTAAACCATTTCTTCAACTTAATGGCAGACCGCAGACCTTCCTCAACCAACTAAACTCTGATCGTCTCCCTCCGAGAACGTCAAAAAGATCCATATTAGCACAATCTGGAAGTCAGGGTTGGGACTTGGGTAGATTCTTGAAAACGTTATTCTTCTTCAATGAACCCCCCTCTCCTGCTAAGATCATTGAGTCTCTAGTCAGCAAACTATCCAACCCGTCACCTAGTGAACCAGTAAAGAGAATGGAAACTTCTGGCACTGTCCTAGTGGCTGGAGCAACTGGTGGTGTAGGTCGAAGGGTGGTCAACATCTTAAGGAAGAAAGGAATGCCAGTTCGAGTATTGGTTAGAAATGAAGAGAAAGCAAGACAGATGTTAGGCCCGGACATTGACTTGATTGTTGGAGACATTACTAAGGAGAGTACTTTGATCCCCAAGTACTTCAAAGGAGTCAGGAGAGTGATTAATGCTGTTTCTGTCATTGTTGGTCCAAAGGAAGGGGACACTCCTGACAGGCAAAAATACAGCCAAGGTATCAAGTTCTTTGAACCAGAGATCAAGGGAGATTCGCCCGAAAAGGTGGAGTACATTGGGATGAAAAATTTGATTAATGCTCTAAAACAAAGTGTTGGACTTCAAAATGGAAAGCTACTATTTGGATATGAAGGTAATAATTATAGGGAATTGACTTGGGGAGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCTGATCGACCCAAATGGCAGTGAAAATGGTGGACCAACTGGACTTTTTAAAGGAGTTGTTTCCACTGCAAATAATGGTGGGTTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTGCGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACAGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCATATGCGCCACCATTTGACCCAAGCAATATTGTGTCACTGCAGGCAATTTTCTACTGCCACTTTTTCTCACTCATTCTTTCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

413

Amino Acids

45.41

Weight (kDa)

9.7

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 118 - 192 1.9e-09 NmrA-like family
NAD_binding_10 PF13460 125 - 241 1.1e-10 NAD(P)H-binding
CIA30 PF08547 262 - 399 6.3e-39 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
AciI CCGC 1 cut(s) 131
AclI AACGTT 1 cut(s) 245
AclWI GGATC 3 cut(s) 183, 523, 969
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 5 cut(s) 523, 539, 686, 835, 1104
AfiI CCNNNNNNNGG 1 cut(s) 596
AflII CTTAAG 1 cut(s) 412
AgsI TTSAA 6 cut(s) 116, 241, 259, 544, 647, 737
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 5 cut(s) 22, 38, 748, 797, 988
AluI AGCT 5 cut(s) 22, 38, 748, 797, 988
Alw26I GTCTC 3 cut(s) 170, 297, 498
AlwI GGATC 3 cut(s) 183, 523, 969
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 2 cut(s) 478, 1127
ApeKI GCWGC 1 cut(s) 988
ApoI RAATTY 1 cut(s) 700
AseI ATTAAT 2 cut(s) 564, 708
AspLEI GCGC 2 cut(s) 993, 1162
AspS9I GGNCC 3 cut(s) 479, 587, 872
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 3 cut(s) 312, 1013, 1052
AvaII GGWCC 2 cut(s) 587, 872
AxyI CCTNAGG 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 975
BccI CCATC 3 cut(s) 808, 971, 998
BceAI ACGGC 1 cut(s) 1121
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 3 cut(s) 170, 297, 498
BfaI CTAG 3 cut(s) 296, 324, 365
BfmI CTRYAG 1 cut(s) 1193
BfrI CTTAAG 1 cut(s) 412
BfuI GTATCC 1 cut(s) 1042
BisI GCNGC 1 cut(s) 989
BlsI GCNGC 1 cut(s) 990
BmcAI AGTACT 2 cut(s) 523, 539
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 2 cut(s) 587, 872
BmgT120I GGNCC 3 cut(s) 479, 587, 872
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 1 cut(s) 939
BplI GAGNNNNNCTC 2 cut(s) 950, 982
BpmI CTGGAG 1 cut(s) 393
Bpu10I CCTNAGC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 628
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse1I ACTGG 5 cut(s) 332, 385, 431, 883, 1090
Bse21I CCTNAGG 1 cut(s) 957
BseDI CCNNGG 1 cut(s) 628
BseGI GGATG 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 48, 948
BseNI ACTGG 5 cut(s) 332, 385, 431, 883, 1090
BseXI GCAGC 1 cut(s) 975
BshFI GGCC 2 cut(s) 480, 1129
BsiSI CCGG 1 cut(s) 482
BslFI GGGAC 2 cut(s) 236, 614
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 3 cut(s) 170, 297, 498
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 236, 614
BsmI GAATGC 1 cut(s) 432
BsnI GGCC 2 cut(s) 480, 1129
Bsp143I GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
BspACI CCGC 1 cut(s) 131
BspANI GGCC 2 cut(s) 480, 1129
BspCNI CTCAG 2 cut(s) 47, 949
BspMAI CTGCAG 1 cut(s) 1197
BspPI GGATC 3 cut(s) 183, 523, 969
BspTI CTTAAG 1 cut(s) 412
BsrI ACTGG 5 cut(s) 332, 385, 431, 883, 1090
BssECI CCNNGG 1 cut(s) 628
BssMI GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
BssT1I CCWWGG 1 cut(s) 628
Bst4CI ACNGT 5 cut(s) 361, 1054, 1081, 1102, 1150
Bst6I CTCTTC 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 412
BstC8I GCNNGC 2 cut(s) 1069, 1197
BstDEI CTNAG 4 cut(s) 34, 279, 513, 957
BstF5I GGATG 1 cut(s) 699
BstHHI GCGC 2 cut(s) 993, 1162
BstKTI GATC 7 cut(s) 163, 191, 285, 531, 657, 846, 964
BstMAI GTCTC 3 cut(s) 170, 297, 498
BstMBI GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 1 cut(s) 1193
BstV1I GCAGC 1 cut(s) 975
BstX2I RGATCY 2 cut(s) 188, 961
BstYI RGATCY 2 cut(s) 188, 961
Bsu36I CCTNAGG 1 cut(s) 957
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 2 cut(s) 480, 1129
BtsCI GGATG 1 cut(s) 699
BtsI GCAGTG 3 cut(s) 865, 903, 1190
BtsIMutI CAGTG 6 cut(s) 357, 865, 903, 1077, 1097, 1190
Cac8I GCNNGC 2 cut(s) 1069, 1197
CaiI CAGNNNCTG 1 cut(s) 65
CfoI GCGC 2 cut(s) 993, 1162
Cfr13I GGNCC 3 cut(s) 479, 587, 872
Csp6I GTAC 5 cut(s) 522, 538, 685, 834, 1103
CviQI GTAC 5 cut(s) 522, 538, 685, 834, 1103
DdeI CTNAG 4 cut(s) 34, 279, 513, 957
DpnI GATC 7 cut(s) 162, 190, 284, 530, 656, 845, 963
DpnII GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
DraI TTTAAA 1 cut(s) 889
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 628
Eco147I AGGCCT 1 cut(s) 1129
Eco47I GGWCC 2 cut(s) 587, 872
Eco57I CTGAAG 1 cut(s) 32
Eco81I CCTNAGG 1 cut(s) 957
EcoT14I CCWWGG 1 cut(s) 628
ErhI CCWWGG 1 cut(s) 628
Esp3I CGTCTC 1 cut(s) 170
FaiI YATR 8 cut(s) 194, 762, 777, 973, 975, 1017, 1156, 1158
FaqI GGGAC 2 cut(s) 236, 614
FauNDI CATATG 2 cut(s) 973, 1156
Fnu4HI GCNGC 1 cut(s) 989
FokI GGATG 1 cut(s) 706
Fsp4HI GCNGC 1 cut(s) 989
FspBI CTAG 3 cut(s) 296, 324, 365
GlaI GCGC 2 cut(s) 992, 1161
GluI GCNGC 1 cut(s) 989
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 2 cut(s) 480, 1129
HapII CCGG 1 cut(s) 482
HhaI GCGC 2 cut(s) 993, 1162
Hin6I GCGC 2 cut(s) 991, 1160
HinP1I GCGC 2 cut(s) 991, 1160
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 5 cut(s) 234, 290, 549, 665, 943
HpaII CCGG 1 cut(s) 482
HphI GGTGA 3 cut(s) 312, 1013, 1052
Hpy166II GTNNAC 4 cut(s) 329, 406, 872, 924
Hpy188I TCNGA 5 cut(s) 160, 174, 843, 935, 1032
Hpy188III TCNNGA 5 cut(s) 50, 207, 238, 553, 608
Hpy8I GTNNAC 4 cut(s) 329, 406, 872, 924
Hpy99I CGWCG 1 cut(s) 1014
HpyAV CCTTC 5 cut(s) 102, 148, 390, 590, 758
HpyCH4III ACNGT 5 cut(s) 361, 1054, 1081, 1102, 1150
HpyCH4IV ACGT 2 cut(s) 179, 245
HpyCH4V TGCA 4 cut(s) 908, 971, 1142, 1195
HpyF3I CTNAG 4 cut(s) 34, 279, 513, 957
HpySE526I ACGT 2 cut(s) 179, 245
HspAI GCGC 2 cut(s) 991, 1160
Kzo9I GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
LmnI GCTCC 2 cut(s) 374, 794
Lsp1109I GCAGC 1 cut(s) 975
LweI GCATC 1 cut(s) 939
MaeI CTAG 3 cut(s) 296, 324, 365
MaeII ACGT 2 cut(s) 179, 245
MaeIII GTNAC 3 cut(s) 318, 1058, 1188
MalI GATC 7 cut(s) 162, 190, 284, 530, 656, 845, 963
MboI GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
MboII GAAGA 8 cut(s) 21, 38, 104, 244, 247, 430, 467, 1110
MflI RGATCY 2 cut(s) 188, 961
MluCI AATT 5 cut(s) 700, 772, 782, 1023, 1200
MlyI GAGTC 2 cut(s) 299, 558
MmeI TCCRAC 3 cut(s) 336, 481, 709
MnlI CCTC 6 cut(s) 43, 152, 180, 280, 952, 1119
MseI TTAA 9 cut(s) 18, 84, 120, 413, 564, 708, 888, 996, 1240
MslI CAYNNNNRTG 1 cut(s) 692
MspCI CTTAAG 1 cut(s) 412
MspI CCGG 1 cut(s) 482
MspR9I CCNGG 1 cut(s) 482
Mva1269I GAATGC 1 cut(s) 432
NciI CCSGG 1 cut(s) 482
NdeI CATATG 2 cut(s) 973, 1156
NdeII GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
NmuCI GTSAC 2 cut(s) 318, 1188
PceI AGGCCT 1 cut(s) 1129
PctI GAATGC 1 cut(s) 432
PfeI GAWTC 3 cut(s) 234, 665, 943
PkrI GCNGC 1 cut(s) 990
PleI GAGTC 2 cut(s) 298, 557
PpsI GAGTC 2 cut(s) 298, 557
PshBI ATTAAT 2 cut(s) 564, 708
PsiI TTATAA 1 cut(s) 1017
Psp1406I AACGTT 1 cut(s) 245
PspPI GGNCC 3 cut(s) 479, 587, 872
PstI CTGCAG 1 cut(s) 1197
PstNI CAGNNNCTG 1 cut(s) 65
PsuI RGATCY 2 cut(s) 188, 961
RsaI GTAC 5 cut(s) 523, 539, 686, 835, 1104
RsaNI GTAC 5 cut(s) 522, 538, 685, 834, 1103
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 9 cut(s) 18, 84, 120, 413, 564, 708, 888, 996, 1240
SatI GCNGC 1 cut(s) 989
Sau3AI GATC 7 cut(s) 160, 188, 282, 528, 654, 843, 961
Sau96I GGNCC 3 cut(s) 479, 587, 872
ScaI AGTACT 2 cut(s) 523, 539
SchI GAGTC 2 cut(s) 299, 558
ScrFI CCNGG 1 cut(s) 482
SfaNI GCATC 1 cut(s) 939
SfcI CTRYAG 1 cut(s) 1193
SinI GGWCC 2 cut(s) 587, 872
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 5 cut(s) 700, 772, 782, 1023, 1200
SseBI AGGCCT 1 cut(s) 1129
SsiI CCGC 1 cut(s) 131
SspI AATATT 1 cut(s) 1183
SspMI CTAG 3 cut(s) 296, 324, 365
StuI AGGCCT 1 cut(s) 1129
StyD4I CCNGG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 5 cut(s) 361, 1054, 1081, 1102, 1150
TaiI ACGT 2 cut(s) 182, 248
TaqI TCGA 3 cut(s) 394, 436, 846
TasI AATT 5 cut(s) 700, 772, 782, 1023, 1200
TatI WGTACW 4 cut(s) 521, 537, 684, 833
TfiI GAWTC 3 cut(s) 234, 665, 943
Tru1I TTAA 9 cut(s) 18, 84, 120, 413, 564, 708, 888, 996, 1240
Tru9I TTAA 9 cut(s) 18, 84, 120, 413, 564, 708, 888, 996, 1240
TscAI CASTG 6 cut(s) 364, 865, 910, 1084, 1097, 1197
TseFI GTSAC 2 cut(s) 318, 1188
TseI GCWGC 1 cut(s) 988
Tsp45I GTSAC 2 cut(s) 318, 1188
TspDTI ATGAA 4 cut(s) 276, 468, 710, 777
TspRI CASTG 6 cut(s) 364, 865, 910, 1084, 1097, 1197
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 2 cut(s) 587, 872
VspI ATTAAT 2 cut(s) 564, 708
XapI RAATTY 1 cut(s) 700
XspI CTAG 3 cut(s) 296, 324, 365
ZrmI AGTACT 2 cut(s) 523, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.