Rh2AG008200

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000908
Physical Location & Seq
Reverse (-)
759755 .. 763137
3383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG008200.1

Sequence Viewer

Length: 651 bp
ATGGGTATTGCTGGAGTTGTTTCCACTGCAAATAACGGTGGGTTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTGCGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACAGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCAGATGCGCCACCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTACCCTGACTTTCAAATTGAAGGTTTATCCTAAACTAATGCGAACTATTTGTTTACTGCATCTTAGTACTCTACTGCTGTTTACATGTCACAAACTGACTCTTGCAATTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

216

Amino Acids

24.14

Weight (kDa)

9.69

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 4 - 130 1.1e-33 Complex I intermediate-associated protein 30 (CIA30)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
AciI CCGC 1 cut(s) 514
AclWI GGATC 2 cut(s) 90, 413
AcsI RAATTY 1 cut(s) 377
AfaI GTAC 3 cut(s) 225, 441, 604
AflIII ACRYGT 2 cut(s) 442, 620
AgsI TTSAA 5 cut(s) 338, 396, 520, 550, 556
AjnI CCWGG 2 cut(s) 431, 478
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 3 cut(s) 109, 319, 385
AluI AGCT 3 cut(s) 109, 319, 385
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 2 cut(s) 90, 413
Ama87I CYCGRG 1 cut(s) 295
AoxI GGCC 2 cut(s) 248, 467
ApeKI GCWGC 2 cut(s) 109, 316
ApoI RAATTY 1 cut(s) 377
AspLEI GCGC 2 cut(s) 114, 283
AsuHPI GGTGA 2 cut(s) 134, 173
AvaI CYCGRG 1 cut(s) 295
AxyI CCTNAGG 1 cut(s) 78
BbvI GCAGC 2 cut(s) 96, 328
BccI CCATC 3 cut(s) 92, 119, 338
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 2 cut(s) 433, 480
BciVI GTATCC 1 cut(s) 163
BcoDI GTCTC 1 cut(s) 469
BfmI CTRYAG 2 cut(s) 314, 453
BfuI GTATCC 1 cut(s) 163
BisI GCNGC 2 cut(s) 110, 317
BlsI GCNGC 2 cut(s) 111, 318
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 2 cut(s) 433, 480
BmeT110I CYCGRG 1 cut(s) 295
BmrFI CCNGG 2 cut(s) 433, 480
BmrI ACTGGG 1 cut(s) 383
BmsI GCATC 3 cut(s) 60, 268, 604
BmuI ACTGGG 1 cut(s) 383
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 1 cut(s) 33
BsaAI YACGTR 1 cut(s) 445
BsaI GGTCTC 1 cut(s) 469
BsaJI CCNNGG 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 211, 389
Bse21I CCTNAGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 433, 480
BseDI CCNNGG 1 cut(s) 431
BseMII CTCAG 2 cut(s) 69, 462
BseNI ACTGG 2 cut(s) 211, 389
BseXI GCAGC 2 cut(s) 96, 328
BshFI GGCC 2 cut(s) 250, 469
BsiHKCI CYCGRG 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 469
BsnI GGCC 2 cut(s) 250, 469
Bso31I GGTCTC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 295
Bsp1407I TGTACA 1 cut(s) 439
Bsp143I GATC 3 cut(s) 82, 418, 487
BspACI CCGC 1 cut(s) 514
BspANI GGCC 2 cut(s) 250, 469
BspCNI CTCAG 2 cut(s) 70, 463
BspMAI CTGCAG 2 cut(s) 318, 457
BspPI GGATC 2 cut(s) 90, 413
BspTNI GGTCTC 1 cut(s) 469
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 2 cut(s) 211, 389
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 3 cut(s) 82, 418, 487
Bst2UI CCWGG 2 cut(s) 433, 480
Bst4CI ACNGT 5 cut(s) 38, 175, 202, 223, 271
BstAUI TGTACA 1 cut(s) 439
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 4 cut(s) 78, 471, 491, 599
BstHHI GCGC 2 cut(s) 114, 283
BstKTI GATC 3 cut(s) 85, 421, 490
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 3 cut(s) 82, 418, 487
BstNI CCWGG 2 cut(s) 433, 480
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 2 cut(s) 431, 478
BstSFI CTRYAG 2 cut(s) 314, 453
BstV1I GCAGC 2 cut(s) 96, 328
BstX2I RGATCY 2 cut(s) 82, 418
BstYI RGATCY 2 cut(s) 82, 418
Bsu36I CCTNAGG 1 cut(s) 78
BsuI GTATCC 1 cut(s) 163
BsuRI GGCC 2 cut(s) 250, 469
BtsI GCAGTG 2 cut(s) 24, 311
BtsIMutI CAGTG 4 cut(s) 24, 198, 218, 311
Cac8I GCNNGC 1 cut(s) 190
CfoI GCGC 2 cut(s) 114, 283
Csp6I GTAC 3 cut(s) 224, 440, 603
CviAII CATG 1 cut(s) 621
CviJI RGCY 8 cut(s) 109, 192, 229, 250, 319, 385, 450, 469
CviKI_1 RGCY 8 cut(s) 109, 192, 229, 250, 319, 385, 450, 469
CviQI GTAC 3 cut(s) 224, 440, 603
DdeI CTNAG 4 cut(s) 78, 471, 491, 599
DpnI GATC 3 cut(s) 84, 420, 489
DpnII GATC 3 cut(s) 82, 418, 487
Eco147I AGGCCT 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 469
Eco81I CCTNAGG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 295
EcoRII CCWGG 2 cut(s) 431, 478
FaeI CATG 1 cut(s) 624
FaiI YATR 9 cut(s) 94, 96, 138, 323, 342, 401, 409, 425, 622
FatI CATG 1 cut(s) 620
FauNDI CATATG 1 cut(s) 94
Fnu4HI GCNGC 2 cut(s) 110, 317
Fsp4HI GCNGC 2 cut(s) 110, 317
GlaI GCGC 2 cut(s) 113, 282
GluI GCNGC 2 cut(s) 110, 317
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 250, 469
HhaI GCGC 2 cut(s) 114, 283
Hin1II CATG 1 cut(s) 624
Hin6I GCGC 2 cut(s) 112, 281
HinP1I GCGC 2 cut(s) 112, 281
HinfI GANTC 2 cut(s) 64, 634
HphI GGTGA 2 cut(s) 134, 173
Hpy166II GTNNAC 5 cut(s) 45, 442, 497, 590, 618
Hpy188I TCNGA 4 cut(s) 56, 153, 277, 414
Hpy8I GTNNAC 5 cut(s) 45, 442, 497, 590, 618
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 2 cut(s) 365, 550
HpyCH4III ACNGT 5 cut(s) 38, 175, 202, 223, 271
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 7 cut(s) 29, 92, 263, 316, 455, 595, 641
HpyF3I CTNAG 4 cut(s) 78, 471, 491, 599
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 1 cut(s) 624
HspAI GCGC 2 cut(s) 112, 281
Kzo9I GATC 3 cut(s) 82, 418, 487
LmnI GCTCC 1 cut(s) 390
Lsp1109I GCAGC 2 cut(s) 96, 328
LweI GCATC 3 cut(s) 60, 268, 604
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 4 cut(s) 179, 309, 460, 623
MalI GATC 3 cut(s) 84, 420, 489
MboI GATC 3 cut(s) 82, 418, 487
MboII GAAGA 1 cut(s) 231
MflI RGATCY 2 cut(s) 82, 418
MluCI AATT 5 cut(s) 144, 377, 527, 551, 642
MlyI GAGTC 1 cut(s) 628
MnlI CCTC 3 cut(s) 73, 240, 513
MseI TTAA 3 cut(s) 117, 381, 645
MspR9I CCNGG 2 cut(s) 433, 480
MvaI CCWGG 2 cut(s) 433, 480
NdeI CATATG 1 cut(s) 94
NdeII GATC 3 cut(s) 82, 418, 487
NlaIII CATG 1 cut(s) 624
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 2 cut(s) 309, 623
NspI RCATGY 1 cut(s) 624
PceI AGGCCT 1 cut(s) 250
PciI ACATGT 1 cut(s) 620
PfeI GAWTC 1 cut(s) 64
PkrI GCNGC 2 cut(s) 111, 318
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 445
PscI ACATGT 1 cut(s) 620
PsiI TTATAA 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 431, 478
PspGI CCWGG 2 cut(s) 431, 478
PstI CTGCAG 2 cut(s) 318, 457
PsuI RGATCY 2 cut(s) 82, 418
RsaI GTAC 3 cut(s) 225, 441, 604
RsaNI GTAC 3 cut(s) 224, 440, 603
SaqAI TTAA 3 cut(s) 117, 381, 645
SatI GCNGC 2 cut(s) 110, 317
Sau3AI GATC 3 cut(s) 82, 418, 487
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 2 cut(s) 433, 480
SfaNI GCATC 3 cut(s) 60, 268, 604
SfcI CTRYAG 2 cut(s) 314, 453
Sse9I AATT 5 cut(s) 144, 377, 527, 551, 642
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 1 cut(s) 514
SspI AATATT 1 cut(s) 304
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 2 cut(s) 431, 478
TaaI ACNGT 5 cut(s) 38, 175, 202, 223, 271
TaiI ACGT 1 cut(s) 447
TasI AATT 5 cut(s) 144, 377, 527, 551, 642
TatI WGTACW 2 cut(s) 439, 602
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 3 cut(s) 117, 381, 645
Tru9I TTAA 3 cut(s) 117, 381, 645
TscAI CASTG 4 cut(s) 31, 205, 218, 318
TseFI GTSAC 2 cut(s) 309, 623
TseI GCWGC 2 cut(s) 109, 316
Tsp45I GTSAC 2 cut(s) 309, 623
TspRI CASTG 4 cut(s) 31, 205, 218, 318
XapI RAATTY 1 cut(s) 377
XceI RCATGY 1 cut(s) 624
ZrmI AGTACT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.