FvH4_3g07310

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000908
Physical Location & Seq
Forward (+)
4343150 .. 4349003
5854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07310.t1

Sequence Viewer

Length: 1779 bp
ATGGAATTTGGTGTCATTAAGCTCTCGTCTTCCTCAGCTCTCAATCTTCAGGATTTTCCATCACCTGTTGGTAGGAAACTTATTAACAAACCTTCACTTCCTAAACCATTTCTTCAACTTAATGGCAGACCACAGACCTTCCTCAGCCAACTAAAATCTGATCGTCTATCTCGGAGAACGTCGAAAAGATCCATAATAGCACAATCTGGAAGCCAGGGTTGGGACTTGGGTAGATTCTTGAAAACGTTATTCTTCTTCAATGAACCACCCTCTCCTGCTAAGATATTTGAGTCTTTAGTCAGCAAAATATCCAGCCCGTCACCCAGTGAATCAGTAAAGAGAATGGAAACTTCTGGCACTGTCCTAGTGGCTGGAGCAACTGGTGGTGTAGGTCGAAGGGTAGTCAACATCTTAAGGAAGAAAGGAATTCCAGTTCGAGTATTGGTTAGAAATGAACAGAAAGCAAGACAGATGTTAGGCCCAGACATTGACTTGATTGTTGGAGACATTACTAAGGAGAGTACTCTGATCCCTGAGTACTTCAAAGGAGTCAGGAAAGTGATTAATGCTGTTTCTGTCATTGTTGGTCCAAAGGAAGGGGACACTCCTGACAGGCAAAAATACAGCCAAGGCATCAAGTTCTTTGAACCAGAGATCAAGGGAGATTCGCCTGAAAAGGTGGAGTACATTGGGATGAAAAATTTGATTAATGCTCTAAAGCAAAGTGTTGGACTTCAAAATGGAAAACTATTATTTGGATATGAAGGTAATAATTGTAGGGAATTGGCTTGGGGAGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCTGATCGACCCAAATGGCAGCGAAGATGGTGGACCAACTGGACTTTTTAAAGGAGTTGTTTCCACCGCAAATAATGGTGGATTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTGCGCCTTAAAGGTGATGGTCGTCGTTATAAGCTAATTGTTCGGACCAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAGGAGACCAATGGCAGACAGTAAGGCTTCCATTTACTTCTTTGAGGCCGATATTTCGTGCAAAAACAGTACCAGATGCGCCATCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTCGTGGAAGGGGCATTTAAGCTCCCAGTTTCAAGCATAAAGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACATGTAAGCTCTGCAGGAGTTACCCGGCCTGAGAGACCTGGACTTGATCTAAGTAGACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAGTTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGAACAAGGAGATAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATATGCAAATGGCAAGACATTTGAGGTTAAAAGTGTTATACCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTACGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTGGAACAAAATCCTGTACCGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

593

Amino Acids

65.01

Weight (kDa)

8.94

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 118 - 192 5.9e-09 NmrA-like family
NAD_binding_10 PF13460 125 - 241 2.6e-10 NAD(P)H-binding
CIA30 PF08547 263 - 422 3.5e-41 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 437 - 536 2.2e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1017
AccI GTMKAC 2 cut(s) 1375, 1671
AciI CCGC 2 cut(s) 906, 1393
AclI AACGTT 1 cut(s) 245
AclWI GGATC 4 cut(s) 183, 523, 969, 1292
AcsI RAATTY 5 cut(s) 5, 426, 700, 1464, 1581
AcuI CTGAAG 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 7 cut(s) 523, 539, 686, 835, 1152, 1320, 1770
AfiI CCNNNNNNNGG 3 cut(s) 220, 596, 1696
AflII CTTAAG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 1321
AgeI ACCGGT 1 cut(s) 1771
AjnI CCWGG 3 cut(s) 213, 1310, 1357
AjuI GAANNNNNNNTTGG 4 cut(s) 202, 234, 1391, 1423
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 8 cut(s) 22, 38, 797, 988, 1021, 1198, 1264, 1329
AluI AGCT 8 cut(s) 22, 38, 797, 988, 1021, 1198, 1264, 1329
Alw26I GTCTC 3 cut(s) 498, 1080, 1348
AlwI GGATC 4 cut(s) 183, 523, 969, 1292
Ama87I CYCGRG 1 cut(s) 1174
AoxI GGCC 4 cut(s) 478, 1127, 1346, 1484
ApeKI GCWGC 4 cut(s) 858, 988, 1195, 1592
ApoI RAATTY 5 cut(s) 5, 426, 700, 1464, 1581
AseI ATTAAT 2 cut(s) 564, 708
AsiGI ACCGGT 1 cut(s) 1771
AspLEI GCGC 2 cut(s) 993, 1162
AspS9I GGNCC 4 cut(s) 479, 587, 872, 1032
AsuC2I CCSGG 1 cut(s) 1345
AsuHPI GGTGA 4 cut(s) 54, 312, 1013, 1052
AvaI CYCGRG 1 cut(s) 1174
AvaII GGWCC 3 cut(s) 587, 872, 1032
AxyI CCTNAGG 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 21
BbvCI CCTCAGC 2 cut(s) 34, 143
BbvI GCAGC 4 cut(s) 870, 975, 1207, 1579
BccI CCATC 8 cut(s) 67, 808, 860, 971, 998, 1171, 1217, 1724
BciT130I CCWGG 3 cut(s) 215, 1312, 1359
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 1 cut(s) 1345
BcoDI GTCTC 3 cut(s) 498, 1080, 1348
BfaI CTAG 1 cut(s) 365
BfmI CTRYAG 3 cut(s) 1080, 1193, 1332
BfrI CTTAAG 1 cut(s) 412
BfuI GTATCC 1 cut(s) 1042
BglII AGATCT 1 cut(s) 1519
BisI GCNGC 4 cut(s) 859, 989, 1196, 1593
BlsI GCNGC 4 cut(s) 860, 990, 1197, 1594
BmcAI AGTACT 2 cut(s) 523, 539
Bme1390I CCNGG 4 cut(s) 215, 1312, 1345, 1359
Bme18I GGWCC 3 cut(s) 587, 872, 1032
BmeT110I CYCGRG 1 cut(s) 1174
BmgT120I GGNCC 4 cut(s) 479, 587, 872, 1032
BmiI GGNNCC 1 cut(s) 1506
BmrFI CCNGG 4 cut(s) 215, 1312, 1345, 1359
BmrI ACTGGG 2 cut(s) 318, 1262
BmsI GCATC 3 cut(s) 642, 939, 1147
BmuI ACTGGG 2 cut(s) 318, 1262
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 4 cut(s) 950, 982, 1506, 1538
BpmI CTGGAG 2 cut(s) 393, 1533
Bpu10I CCTNAGC 2 cut(s) 34, 143
BpuMI CCSGG 1 cut(s) 1345
BsaI GGTCTC 2 cut(s) 1080, 1348
BsaJI CCNNGG 3 cut(s) 214, 628, 1310
BsaWI WCCGGW 1 cut(s) 1771
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 596, 1696
Bse118I RCCGGY 1 cut(s) 1771
Bse1I ACTGG 6 cut(s) 324, 385, 431, 883, 1035, 1268
Bse21I CCTNAGG 1 cut(s) 957
BseBI CCWGG 3 cut(s) 215, 1312, 1359
BseDI CCNNGG 3 cut(s) 214, 628, 1310
BseGI GGATG 2 cut(s) 699, 1735
BseLI CCNNNNNNNGG 3 cut(s) 220, 596, 1696
BseMII CTCAG 7 cut(s) 48, 157, 525, 948, 1341, 1491, 1683
BseNI ACTGG 6 cut(s) 324, 385, 431, 883, 1035, 1268
BseRI GAGGAG 1 cut(s) 1517
BseXI GCAGC 4 cut(s) 870, 975, 1207, 1579
BshFI GGCC 4 cut(s) 480, 1129, 1348, 1486
BshTI ACCGGT 1 cut(s) 1771
BsiHKCI CYCGRG 1 cut(s) 1174
BsiSI CCGG 2 cut(s) 1345, 1772
BslFI GGGAC 2 cut(s) 236, 614
BslI CCNNNNNNNGG 3 cut(s) 220, 596, 1696
BsmAI GTCTC 3 cut(s) 498, 1080, 1348
BsmFI GGGAC 2 cut(s) 236, 614
BsnI GGCC 4 cut(s) 480, 1129, 1348, 1486
Bso31I GGTCTC 2 cut(s) 1080, 1348
BsoBI CYCGRG 1 cut(s) 1174
Bsp1407I TGTACA 1 cut(s) 1318
Bsp143I GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
BspACI CCGC 2 cut(s) 906, 1393
BspANI GGCC 4 cut(s) 480, 1129, 1348, 1486
BspCNI CTCAG 7 cut(s) 47, 156, 526, 949, 1342, 1492, 1684
BspLI GGNNCC 1 cut(s) 1506
BspMAI CTGCAG 2 cut(s) 1197, 1336
BspPI GGATC 4 cut(s) 183, 523, 969, 1292
BspTI CTTAAG 1 cut(s) 412
BspTNI GGTCTC 2 cut(s) 1080, 1348
BsrFI RCCGGY 1 cut(s) 1771
BsrGI TGTACA 1 cut(s) 1318
BsrI ACTGG 6 cut(s) 324, 385, 431, 883, 1035, 1268
BssAI RCCGGY 1 cut(s) 1771
BssECI CCNNGG 3 cut(s) 214, 628, 1310
BssMI GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
BssT1I CCWWGG 1 cut(s) 628
Bst2UI CCWGG 3 cut(s) 215, 1312, 1359
Bst4CI ACNGT 6 cut(s) 361, 1054, 1081, 1102, 1150, 1669
Bst6I CTCTTC 1 cut(s) 1563
BstAFI CTTAAG 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 1318
BstC8I GCNNGC 3 cut(s) 1069, 1509, 1658
BstF5I GGATG 2 cut(s) 699, 1735
BstHHI GCGC 2 cut(s) 993, 1162
BstKTI GATC 9 cut(s) 163, 191, 531, 657, 846, 964, 1300, 1369, 1522
BstMAI GTCTC 3 cut(s) 498, 1080, 1348
BstMBI GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
BstMWI GCNNNNNNNGC 1 cut(s) 794
BstNI CCWGG 3 cut(s) 215, 1312, 1359
BstNSI RCATGY 1 cut(s) 1325
BstSCI CCNGG 4 cut(s) 213, 1310, 1343, 1357
BstSFI CTRYAG 3 cut(s) 1080, 1193, 1332
BstV1I GCAGC 4 cut(s) 870, 975, 1207, 1579
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 4 cut(s) 188, 961, 1297, 1519
BstYI RGATCY 4 cut(s) 188, 961, 1297, 1519
Bsu36I CCTNAGG 1 cut(s) 957
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 4 cut(s) 480, 1129, 1348, 1486
BtsCI GGATG 2 cut(s) 699, 1735
BtsI GCAGTG 1 cut(s) 1190
BtsIMutI CAGTG 5 cut(s) 331, 357, 1042, 1077, 1190
Cac8I GCNNGC 3 cut(s) 1069, 1509, 1658
CfoI GCGC 2 cut(s) 993, 1162
Cfr10I RCCGGY 1 cut(s) 1771
Cfr13I GGNCC 4 cut(s) 479, 587, 872, 1032
Csp6I GTAC 7 cut(s) 522, 538, 685, 834, 1151, 1319, 1769
CspAI ACCGGT 1 cut(s) 1771
CviAII CATG 1 cut(s) 1322
CviQI GTAC 7 cut(s) 522, 538, 685, 834, 1151, 1319, 1769
DpnI GATC 9 cut(s) 162, 190, 530, 656, 845, 963, 1299, 1368, 1521
DpnII GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
DraI TTTAAA 2 cut(s) 889, 1717
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 1563
EarI CTCTTC 1 cut(s) 1563
Eco130I CCWWGG 1 cut(s) 628
Eco147I AGGCCT 1 cut(s) 1486
Eco31I GGTCTC 2 cut(s) 1080, 1348
Eco32I GATATC 1 cut(s) 1560
Eco47I GGWCC 3 cut(s) 587, 872, 1032
Eco57I CTGAAG 1 cut(s) 32
Eco81I CCTNAGG 1 cut(s) 957
Eco88I CYCGRG 1 cut(s) 1174
EcoRI GAATTC 2 cut(s) 426, 1464
EcoRII CCWGG 3 cut(s) 213, 1310, 1357
EcoRV GATATC 1 cut(s) 1560
EcoT14I CCWWGG 1 cut(s) 628
ErhI CCWWGG 1 cut(s) 628
FaeI CATG 1 cut(s) 1325
FaqI GGGAC 2 cut(s) 236, 614
FatI CATG 1 cut(s) 1321
FauNDI CATATG 2 cut(s) 973, 1609
FblI GTMKAC 2 cut(s) 1375, 1671
Fnu4HI GCNGC 4 cut(s) 859, 989, 1196, 1593
FokI GGATG 2 cut(s) 706, 1742
Fsp4HI GCNGC 4 cut(s) 859, 989, 1196, 1593
FspBI CTAG 1 cut(s) 365
GlaI GCGC 2 cut(s) 992, 1161
GluI GCNGC 4 cut(s) 859, 989, 1196, 1593
GsuI CTGGAG 2 cut(s) 393, 1533
HaeIII GGCC 4 cut(s) 480, 1129, 1348, 1486
HapII CCGG 2 cut(s) 1345, 1772
HhaI GCGC 2 cut(s) 993, 1162
Hin1II CATG 1 cut(s) 1325
Hin6I GCGC 2 cut(s) 991, 1160
HinP1I GCGC 2 cut(s) 991, 1160
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 6 cut(s) 234, 290, 329, 549, 665, 943
HpaII CCGG 2 cut(s) 1345, 1772
HphI GGTGA 4 cut(s) 54, 312, 1013, 1052
Hpy166II GTNNAC 5 cut(s) 406, 872, 1376, 1672, 1711
Hpy188I TCNGA 7 cut(s) 160, 174, 528, 843, 935, 1032, 1293
Hpy188III TCNNGA 8 cut(s) 50, 207, 238, 553, 608, 1420, 1454, 1563
Hpy8I GTNNAC 5 cut(s) 406, 872, 1376, 1672, 1711
Hpy99I CGWCG 2 cut(s) 184, 1014
HpyAV CCTTC 9 cut(s) 102, 148, 390, 590, 758, 1244, 1429, 1690, 1720
HpyCH4III ACNGT 6 cut(s) 361, 1054, 1081, 1102, 1150, 1669
HpyCH4IV ACGT 2 cut(s) 179, 245
HpyCH4V TGCA 7 cut(s) 971, 1142, 1195, 1334, 1493, 1595, 1613
HpyF10VI GCNNNNNNNGC 1 cut(s) 794
HpySE526I ACGT 2 cut(s) 179, 245
Hsp92II CATG 1 cut(s) 1325
HspAI GCGC 2 cut(s) 991, 1160
Kzo9I GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
LmnI GCTCC 5 cut(s) 374, 794, 1269, 1504, 1514
Lsp1109I GCAGC 4 cut(s) 870, 975, 1207, 1579
LweI GCATC 3 cut(s) 642, 939, 1147
MaeI CTAG 1 cut(s) 365
MaeII ACGT 2 cut(s) 179, 245
MaeIII GTNAC 4 cut(s) 318, 1058, 1188, 1339
MalI GATC 9 cut(s) 162, 190, 530, 656, 845, 963, 1299, 1368, 1521
MboI GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
MboII GAAGA 8 cut(s) 21, 38, 104, 244, 247, 430, 875, 1580
MfeI CAATTG 1 cut(s) 1476
MflI RGATCY 4 cut(s) 188, 961, 1297, 1519
MlyI GAGTC 2 cut(s) 299, 558
MmeI TCCRAC 2 cut(s) 481, 709
MslI CAYNNNNRTG 1 cut(s) 692
MspCI CTTAAG 1 cut(s) 412
MspI CCGG 2 cut(s) 1345, 1772
MspR9I CCNGG 4 cut(s) 215, 1312, 1345, 1359
MunI CAATTG 1 cut(s) 1476
MvaI CCWGG 3 cut(s) 215, 1312, 1359
MwoI GCNNNNNNNGC 1 cut(s) 794
NciI CCSGG 1 cut(s) 1345
NdeI CATATG 2 cut(s) 973, 1609
NdeII GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
NlaIII CATG 1 cut(s) 1325
NlaIV GGNNCC 1 cut(s) 1506
NmuCI GTSAC 2 cut(s) 318, 1188
NspI RCATGY 1 cut(s) 1325
PceI AGGCCT 1 cut(s) 1486
PciI ACATGT 1 cut(s) 1321
PfeI GAWTC 4 cut(s) 234, 329, 665, 943
PinAI ACCGGT 1 cut(s) 1771
PkrI GCNGC 4 cut(s) 860, 990, 1197, 1594
PleI GAGTC 2 cut(s) 298, 557
PpsI GAGTC 2 cut(s) 298, 557
PscI ACATGT 1 cut(s) 1321
PshBI ATTAAT 2 cut(s) 564, 708
PsiI TTATAA 1 cut(s) 1017
Psp1406I AACGTT 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 213, 1310, 1357
PspGI CCWGG 3 cut(s) 213, 1310, 1357
PspN4I GGNNCC 1 cut(s) 1506
PspPI GGNCC 4 cut(s) 479, 587, 872, 1032
PstI CTGCAG 2 cut(s) 1197, 1336
PsuI RGATCY 4 cut(s) 188, 961, 1297, 1519
RsaI GTAC 7 cut(s) 523, 539, 686, 835, 1152, 1320, 1770
RsaNI GTAC 7 cut(s) 522, 538, 685, 834, 1151, 1319, 1769
RseI CAYNNNNRTG 1 cut(s) 692
SatI GCNGC 4 cut(s) 859, 989, 1196, 1593
Sau3AI GATC 9 cut(s) 160, 188, 528, 654, 843, 961, 1297, 1366, 1519
Sau96I GGNCC 4 cut(s) 479, 587, 872, 1032
ScaI AGTACT 2 cut(s) 523, 539
SchI GAGTC 2 cut(s) 299, 558
ScrFI CCNGG 4 cut(s) 215, 1312, 1345, 1359
SfaNI GCATC 3 cut(s) 642, 939, 1147
SfcI CTRYAG 3 cut(s) 1080, 1193, 1332
SinI GGWCC 3 cut(s) 587, 872, 1032
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
SseBI AGGCCT 1 cut(s) 1486
SsiI CCGC 2 cut(s) 906, 1393
SspI AATATT 1 cut(s) 1183
SspMI CTAG 1 cut(s) 365
StuI AGGCCT 1 cut(s) 1486
StyD4I CCNGG 4 cut(s) 213, 1310, 1343, 1357
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 6 cut(s) 361, 1054, 1081, 1102, 1150, 1669
TaiI ACGT 2 cut(s) 182, 248
TaqI TCGA 4 cut(s) 182, 394, 436, 846
TatI WGTACW 5 cut(s) 521, 537, 684, 833, 1318
TfiI GAWTC 4 cut(s) 234, 329, 665, 943
TscAI CASTG 5 cut(s) 331, 364, 1042, 1084, 1197
TseFI GTSAC 2 cut(s) 318, 1188
TseI GCWGC 4 cut(s) 858, 988, 1195, 1592
Tsp45I GTSAC 2 cut(s) 318, 1188
TspDTI ATGAA 4 cut(s) 276, 468, 710, 777
TspRI CASTG 5 cut(s) 331, 364, 1042, 1084, 1197
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 3 cut(s) 587, 872, 1032
VspI ATTAAT 2 cut(s) 564, 708
XapI RAATTY 5 cut(s) 5, 426, 700, 1464, 1581
XceI RCATGY 1 cut(s) 1325
XmiI GTMKAC 2 cut(s) 1375, 1671
XspI CTAG 1 cut(s) 365
ZrmI AGTACT 2 cut(s) 523, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.