MD05G1295400.v1.1

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000908
Physical Location & Seq
Forward (+)
43006451 .. 43011012
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1295400.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGGAATTAGGTGCCATTAATCTGTCGTCCCCTTTGTCTCCATCAGCCCTCAGAATTCAGGATTTTTCATCACCTGTTGGTAGGAATCATTTTAAAAAATCTTTACATCCTCAGTTGCTGTGCTCTCCACTTCCTAAACCCTTTCTTCAACTTAATGACAGACCACATAGCTTTCTCTCCCAACAAAACACTGATAATCTCTCTACGAGAACGTATAGAAGATACATATCAGCAGAAGCTGGAAAGCAAAGTTGGGACTTGACTAGATTCTTGAAAACAATATATTTCTTCAACGAACCGCCCTCTCCTGCTAAGTTTATTGAGGCTTTAATTGAAAAACTATCCGGCTCATCACCTAGTAAACCAGAAAGGAAAATGGAAACTTCTGGCATTGTCCTAGTGGCTGGAGCAACCGGTGGTGTAGGTCGAAGGGCAGTCAACATCTTAAGGAAGAAAGGGTTTCCAGTTCGAGTATTGGTTGTTGGAGACATTACTAAGGAGAGTACACTAGTTCCTGAGTACTTCAAAGGTGTGAGGAGAGTGATTAATGCGGTTTCTGTCATTGTTGGTCCAAAGGAAGGTGACACTCCTGATAGGCAAAAGTATAGCCAGATAAAGGGGGATTCACCCGAAAAGGTGGAGTACATTGGGATGAAAAATTTGATCAATGCTGTAAAAGGAAGCGTCGGACTTCAAAATGGGAAAATACTTTTTGGATTCGAAGGTAATAGTTTTAAGGAAATGACTTGGGGTGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCAGATTGACCGAAACGGTGGTGAAAATGGTGGACCAACTGGACTTTTTAAAGGGGTTGTTTCCACTGCAAACAATGGTGGTTTTTCTAGTATCAGAACAAAGAATCTACCAACAGCTGAGGATCTCTCTGCATATGATGGCTTGGAGCTACGCCTTAAAGGTGATGGTCGACGCTATAAGTTAATTGTTCGCACAAGCCGTGATTGGGATACTGTTGGTTACACAGCAGGTTTTGACACTGTTGGAAACCAGTGGCAGACGGTACGCATACCATTTTCTTCTTTGAAACCAATATTTCGAGCACGAACTGTATCAGATGCACCACCGTTTGACCCGAGCAACGTTGTGTCGTTGCAGCTCATGTTTAGCAAGTTTGAATACGATGGGAAACTAAATCCTACTTTCGTGGAAGGCCCATTTCAGCTCCCATTATCAAGCATACGGGCATATCTAAAAGAACCTATAACTCCCAGGTTTGTACATGTAGGTTCTGCAGGAGTCACCCGACCTGAGAGGCCTGGACTTGACCTAAGTAAACAACCTCCCGCTGTTCGCTTGAACAAGGAATTGGATTTTATCCTTACATACAAGTTGAAGGGGGAGGATTTAATACGGGAAAGTGGAATACCATACACAATTGTGAGGCCTTGTGCATTAACCGAGGAGCCTGCCGGAGCAGATCTCATTTTTGATCAAGGAGACAATATAACGGGTAAGATTTCAAGAGAGGAGGTTGCTCAAATTTGCGTTGCTGCTTTAGAAAGCCCGTATGCAACTGGCAAGACATTTGAGGTTAAGAGTGTCATACCATTTAGCCAGCCATTTACAGTGGATCCCGAAAACCCACCTCCTGAGAAGGACTACGATGTTTACTTTAAAACTTTGAAAGATGGAATTACGGGAAAGGAAATCTTGGAACAAGACCCTGTACCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

579

Amino Acids

63.66

Weight (kDa)

8.95

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 241 - 408 2.4e-44 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 422 - 522 3.3e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 11
AccI GTMKAC 1 cut(s) 967
AciI CCGC 3 cut(s) 299, 551, 1344
AclI AACGTT 1 cut(s) 1140
AclWI GGATC 3 cut(s) 927, 1625, 1638
AcsI RAATTY 3 cut(s) 54, 658, 1539
AfaI GTAC 7 cut(s) 505, 521, 644, 793, 1062, 1278, 1728
AfiI CCNNNNNNNGG 3 cut(s) 578, 616, 1003
AflII CTTAAG 1 cut(s) 445
AflIII ACRYGT 1 cut(s) 1279
AgeI ACCGGT 1 cut(s) 413
AhlI ACTAGT 1 cut(s) 508
AjnI CCWGG 2 cut(s) 1268, 1315
AjuI GAANNNNNNNTTGG 4 cut(s) 235, 267, 1349, 1381
AleI CACNNNNGTG 1 cut(s) 1436
AluBI AGCT 6 cut(s) 171, 239, 914, 946, 1156, 1222
AluI AGCT 6 cut(s) 171, 239, 914, 946, 1156, 1222
Alw21I GWGCWC 2 cut(s) 125, 1102
Alw26I GTCTC 3 cut(s) 42, 480, 1491
AlwI GGATC 3 cut(s) 927, 1625, 1638
AlwNI CAGNNNCTG 2 cut(s) 118, 239
Ama87I CYCGRG 1 cut(s) 1132
AoxI GGCC 3 cut(s) 1210, 1313, 1442
ApeKI GCWGC 2 cut(s) 1153, 1550
ApoI RAATTY 3 cut(s) 54, 658, 1539
AseI ATTAAT 2 cut(s) 18, 546
AsiGI ACCGGT 1 cut(s) 413
AspS9I GGNCC 3 cut(s) 569, 830, 1211
AsuHPI GGTGA 7 cut(s) 63, 345, 593, 618, 830, 971, 1291
AsuII TTCGAA 1 cut(s) 720
AvaI CYCGRG 1 cut(s) 1132
AvaII GGWCC 2 cut(s) 569, 830
BamHI GGATCC 1 cut(s) 1630
BanI GGYRCC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 125, 1102
BbvCI CCTCAGC 1 cut(s) 915
BbvI GCAGC 2 cut(s) 1165, 1537
BccI CCATC 6 cut(s) 49, 766, 929, 956, 1175, 1682
BceAI ACGGC 1 cut(s) 981
BcgI CGANNNNNNTGC 2 cut(s) 1448, 1482
BciT130I CCWGG 2 cut(s) 1270, 1317
BciVI GTATCC 1 cut(s) 1000
BclI TGATCA 2 cut(s) 663, 1489
BcoDI GTCTC 3 cut(s) 42, 480, 1491
BcuI ACTAGT 1 cut(s) 508
BfaI CTAG 5 cut(s) 264, 357, 398, 509, 885
BfmI CTRYAG 1 cut(s) 1290
BfrI CTTAAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 1016
BfuI GTATCC 1 cut(s) 1000
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 2 cut(s) 1154, 1551
BlsI GCNGC 2 cut(s) 1155, 1552
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 2 cut(s) 1270, 1317
Bme18I GGWCC 2 cut(s) 569, 830
BmeT110I CYCGRG 1 cut(s) 1132
BmgT120I GGNCC 3 cut(s) 569, 830, 1211
BmiI GGNNCC 3 cut(s) 13, 1464, 1632
BmrFI CCNGG 2 cut(s) 1270, 1317
BmsI GCATC 1 cut(s) 1105
BplI GAGNNNNNCTC 4 cut(s) 908, 940, 1464, 1496
BpmI CTGGAG 1 cut(s) 426
Bpu10I CCTNAGC 1 cut(s) 915
Bpu14I TTCGAA 1 cut(s) 720
BsaBI GATNNNNATC 1 cut(s) 226
BsaJI CCNNGG 2 cut(s) 1268, 1458
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 3 cut(s) 578, 616, 1003
Bse118I RCCGGY 1 cut(s) 413
Bse1I ACTGG 5 cut(s) 464, 841, 1048, 1579, 1730
Bse8I GATNNNNATC 1 cut(s) 226
BseBI CCWGG 2 cut(s) 1270, 1317
BseDI CCNNGG 2 cut(s) 1268, 1458
BseGI GGATG 2 cut(s) 106, 657
BseJI GATNNNNATC 1 cut(s) 226
BseLI CCNNNNNNNGG 3 cut(s) 578, 616, 1003
BseMII CTCAG 6 cut(s) 64, 125, 507, 906, 1299, 1641
BseNI ACTGG 5 cut(s) 464, 841, 1048, 1579, 1730
BseRI GAGGAG 3 cut(s) 550, 1475, 1541
BseXI GCAGC 2 cut(s) 1165, 1537
BshFI GGCC 3 cut(s) 1212, 1315, 1444
BshNI GGYRCC 1 cut(s) 11
BshTI ACCGGT 1 cut(s) 413
BsiHKAI GWGCWC 2 cut(s) 125, 1102
BsiHKCI CYCGRG 1 cut(s) 1132
BsiSI CCGG 3 cut(s) 345, 414, 1470
BslFI GGGAC 2 cut(s) 13, 269
BslI CCNNNNNNNGG 3 cut(s) 578, 616, 1003
BsmAI GTCTC 3 cut(s) 42, 480, 1491
BsmFI GGGAC 2 cut(s) 13, 269
BsnI GGCC 3 cut(s) 1212, 1315, 1444
BsoBI CYCGRG 1 cut(s) 1132
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 125, 1102
Bsp1407I TGTACA 1 cut(s) 1276
Bsp143I GATC 5 cut(s) 663, 919, 1477, 1489, 1630
BspACI CCGC 3 cut(s) 299, 551, 1344
BspANI GGCC 3 cut(s) 1212, 1315, 1444
BspCNI CTCAG 6 cut(s) 63, 124, 508, 907, 1300, 1642
BspLI GGNNCC 3 cut(s) 13, 1464, 1632
BspMAI CTGCAG 1 cut(s) 1294
BspMI ACCTGC 1 cut(s) 1016
BspPI GGATC 3 cut(s) 927, 1625, 1638
BspT104I TTCGAA 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 11
BspTI CTTAAG 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 413
BsrGI TGTACA 1 cut(s) 1276
BsrI ACTGG 5 cut(s) 464, 841, 1048, 1579, 1730
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 1268, 1458
BssMI GATC 5 cut(s) 663, 919, 1477, 1489, 1630
Bst2UI CCWGG 2 cut(s) 1270, 1317
Bst4CI ACNGT 7 cut(s) 815, 1012, 1039, 1060, 1108, 1125, 1627
BstAFI CTTAAG 1 cut(s) 445
BstAUI TGTACA 1 cut(s) 1276
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 2 cut(s) 1467, 1616
BstDEI CTNAG 9 cut(s) 50, 111, 312, 495, 516, 915, 1308, 1328, 1650
BstF5I GGATG 2 cut(s) 106, 657
BstKTI GATC 5 cut(s) 666, 922, 1480, 1492, 1633
BstMAI GTCTC 3 cut(s) 42, 480, 1491
BstMBI GATC 5 cut(s) 663, 919, 1477, 1489, 1630
BstNI CCWGG 2 cut(s) 1270, 1317
BstNSI RCATGY 1 cut(s) 1283
BstSCI CCNGG 2 cut(s) 1268, 1315
BstSFI CTRYAG 1 cut(s) 1290
BstV1I GCAGC 2 cut(s) 1165, 1537
BstX2I RGATCY 3 cut(s) 919, 1477, 1630
BstYI RGATCY 3 cut(s) 919, 1477, 1630
BsuI GTATCC 1 cut(s) 1000
BsuRI GGCC 3 cut(s) 1212, 1315, 1444
BtsCI GGATG 2 cut(s) 106, 657
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 6 cut(s) 189, 861, 1035, 1055, 1632, 1737
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 2 cut(s) 1467, 1616
CaiI CAGNNNCTG 2 cut(s) 118, 239
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 3 cut(s) 569, 830, 1211
CseI GACGC 2 cut(s) 673, 978
Csp6I GTAC 7 cut(s) 504, 520, 643, 792, 1061, 1277, 1727
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 2 cut(s) 1159, 1280
CviQI GTAC 7 cut(s) 504, 520, 643, 792, 1061, 1277, 1727
DdeI CTNAG 9 cut(s) 50, 111, 312, 495, 516, 915, 1308, 1328, 1650
DpnI GATC 5 cut(s) 665, 921, 1479, 1491, 1632
DpnII GATC 5 cut(s) 663, 919, 1477, 1489, 1630
DraI TTTAAA 3 cut(s) 94, 847, 1675
Eco147I AGGCCT 2 cut(s) 1315, 1444
Eco47I GGWCC 2 cut(s) 569, 830
Eco88I CYCGRG 1 cut(s) 1132
EcoRI GAATTC 1 cut(s) 54
EcoRII CCWGG 2 cut(s) 1268, 1315
FaeI CATG 2 cut(s) 1162, 1283
FalI AAGNNNNNCTT 2 cut(s) 1694, 1726
FaqI GGGAC 2 cut(s) 13, 269
FatI CATG 2 cut(s) 1158, 1279
FauI CCCGC 1 cut(s) 1351
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 2 cut(s) 663, 1489
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 2 cut(s) 1154, 1551
FokI GGATG 2 cut(s) 93, 664
Fsp4HI GCNGC 2 cut(s) 1154, 1551
FspBI CTAG 5 cut(s) 264, 357, 398, 509, 885
GluI GCNGC 2 cut(s) 1154, 1551
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 3 cut(s) 1212, 1315, 1444
HapII CCGG 3 cut(s) 345, 414, 1470
HgaI GACGC 2 cut(s) 673, 978
Hin1II CATG 2 cut(s) 1162, 1283
HincII GTYRAC 2 cut(s) 439, 968
HindII GTYRAC 2 cut(s) 439, 968
HinfI GANTC 6 cut(s) 85, 267, 623, 717, 901, 1296
HpaII CCGG 3 cut(s) 345, 414, 1470
HphI GGTGA 7 cut(s) 63, 345, 593, 618, 830, 971, 1291
Hpy166II GTNNAC 7 cut(s) 362, 439, 506, 830, 968, 1334, 1669
Hpy188I TCNGA 5 cut(s) 53, 689, 801, 893, 1114
Hpy188III TCNNGA 7 cut(s) 59, 271, 515, 590, 1521, 1634, 1649
Hpy8I GTNNAC 7 cut(s) 362, 439, 506, 830, 968, 1334, 1669
Hpy99I CGWCG 2 cut(s) 689, 972
HpyAV CCTTC 6 cut(s) 423, 572, 716, 1202, 1387, 1648
HpyCH4III ACNGT 7 cut(s) 815, 1012, 1039, 1060, 1108, 1125, 1627
HpyCH4IV ACGT 2 cut(s) 212, 1140
HpyCH4V TGCA 7 cut(s) 866, 929, 1118, 1153, 1292, 1451, 1571
HpyF3I CTNAG 9 cut(s) 50, 111, 312, 495, 516, 915, 1308, 1328, 1650
HpySE526I ACGT 2 cut(s) 212, 1140
Hsp92II CATG 2 cut(s) 1162, 1283
Ksp22I TGATCA 2 cut(s) 663, 1489
Kzo9I GATC 5 cut(s) 663, 919, 1477, 1489, 1630
LmnI GCTCC 5 cut(s) 407, 943, 1227, 1462, 1472
Lsp1109I GCAGC 2 cut(s) 1165, 1537
LweI GCATC 1 cut(s) 1105
MaeI CTAG 5 cut(s) 264, 357, 398, 509, 885
MaeII ACGT 2 cut(s) 212, 1140
MaeIII GTNAC 3 cut(s) 581, 1016, 1297
MalI GATC 5 cut(s) 665, 921, 1479, 1491, 1632
MboI GATC 5 cut(s) 663, 919, 1477, 1489, 1630
MboII GAAGA 5 cut(s) 137, 231, 280, 463, 1068
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 3 cut(s) 919, 1477, 1630
MhlI GDGCHC 2 cut(s) 125, 1102
MluCI AATT 9 cut(s) 5, 54, 330, 658, 981, 1364, 1434, 1539, 1692
MlyI GAGTC 1 cut(s) 1305
MmeI TCCRAC 3 cut(s) 463, 667, 1021
MslI CAYNNNNRTG 2 cut(s) 650, 1436
MspA1I CMGCKG 2 cut(s) 914, 1346
MspCI CTTAAG 1 cut(s) 445
MspI CCGG 3 cut(s) 345, 414, 1470
MspR9I CCNGG 2 cut(s) 1270, 1317
MunI CAATTG 1 cut(s) 1434
MvaI CCWGG 2 cut(s) 1270, 1317
NdeI CATATG 1 cut(s) 931
NdeII GATC 5 cut(s) 663, 919, 1477, 1489, 1630
NlaIII CATG 2 cut(s) 1162, 1283
NlaIV GGNNCC 3 cut(s) 13, 1464, 1632
NmuCI GTSAC 2 cut(s) 581, 1297
NspI RCATGY 1 cut(s) 1283
NspV TTCGAA 1 cut(s) 720
OliI CACNNNNGTG 1 cut(s) 1436
PceI AGGCCT 2 cut(s) 1315, 1444
PciI ACATGT 1 cut(s) 1279
PcsI WCGNNNNNNNCGW 1 cut(s) 994
PfeI GAWTC 5 cut(s) 85, 267, 623, 717, 901
PinAI ACCGGT 1 cut(s) 413
PkrI GCNGC 2 cut(s) 1155, 1552
PleI GAGTC 1 cut(s) 1304
PpsI GAGTC 1 cut(s) 1304
PscI ACATGT 1 cut(s) 1279
PshBI ATTAAT 2 cut(s) 18, 546
Psp1406I AACGTT 1 cut(s) 1140
Psp6I CCWGG 2 cut(s) 1268, 1315
PspGI CCWGG 2 cut(s) 1268, 1315
PspN4I GGNNCC 3 cut(s) 13, 1464, 1632
PspPI GGNCC 3 cut(s) 569, 830, 1211
PstI CTGCAG 1 cut(s) 1294
PstNI CAGNNNCTG 2 cut(s) 118, 239
PsuI RGATCY 3 cut(s) 919, 1477, 1630
PvuII CAGCTG 1 cut(s) 914
RsaI GTAC 7 cut(s) 505, 521, 644, 793, 1062, 1278, 1728
RsaNI GTAC 7 cut(s) 504, 520, 643, 792, 1061, 1277, 1727
RseI CAYNNNNRTG 2 cut(s) 650, 1436
SalI GTCGAC 1 cut(s) 966
SatI GCNGC 2 cut(s) 1154, 1551
Sau3AI GATC 5 cut(s) 663, 919, 1477, 1489, 1630
Sau96I GGNCC 3 cut(s) 569, 830, 1211
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 1305
ScrFI CCNGG 2 cut(s) 1270, 1317
SduI GDGCHC 2 cut(s) 125, 1102
SfaNI GCATC 1 cut(s) 1105
SfcI CTRYAG 1 cut(s) 1290
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 2 cut(s) 569, 830
SmiMI CAYNNNNRTG 2 cut(s) 650, 1436
SmlI CTYRAG 1 cut(s) 445
SmoI CTYRAG 1 cut(s) 445
SpeI ACTAGT 1 cut(s) 508
Sse9I AATT 9 cut(s) 5, 54, 330, 658, 981, 1364, 1434, 1539, 1692
SseBI AGGCCT 2 cut(s) 1315, 1444
SsiI CCGC 3 cut(s) 299, 551, 1344
SspI AATATT 1 cut(s) 1092
SspMI CTAG 5 cut(s) 264, 357, 398, 509, 885
StuI AGGCCT 2 cut(s) 1315, 1444
StyD4I CCNGG 2 cut(s) 1268, 1315
TaaI ACNGT 7 cut(s) 815, 1012, 1039, 1060, 1108, 1125, 1627
TaiI ACGT 2 cut(s) 215, 1143
TaqI TCGA 5 cut(s) 427, 469, 720, 967, 1096
TaqII GACCGA 1 cut(s) 822
TasI AATT 9 cut(s) 5, 54, 330, 658, 981, 1364, 1434, 1539, 1692
TatI WGTACW 5 cut(s) 503, 519, 642, 791, 1276
TfiI GAWTC 5 cut(s) 85, 267, 623, 717, 901
TscAI CASTG 6 cut(s) 196, 868, 1042, 1055, 1632, 1737
TseFI GTSAC 2 cut(s) 581, 1297
TseI GCWGC 2 cut(s) 1153, 1550
Tsp45I GTSAC 2 cut(s) 581, 1297
TspDTI ATGAA 2 cut(s) 57, 668
TspRI CASTG 6 cut(s) 196, 868, 1042, 1055, 1632, 1737
Vha464I CTTAAG 1 cut(s) 445
VpaK11BI GGWCC 2 cut(s) 569, 830
VspI ATTAAT 2 cut(s) 18, 546
XapI RAATTY 3 cut(s) 54, 658, 1539
XceI RCATGY 1 cut(s) 1283
XmiI GTMKAC 1 cut(s) 967
XspI CTAG 5 cut(s) 264, 357, 398, 509, 885
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.