NAD(P)H-binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1017 |
| AciI | CCGC | 2 cut(s) | 131, 1393 |
| AclI | AACGTT | 1 cut(s) | 245 |
| AclWI | GGATC | 3 cut(s) | 183, 969, 1292 |
| AcsI | RAATTY | 3 cut(s) | 700, 1256, 1464 |
| AcuI | CTGAAG | 1 cut(s) | 32 |
| AdeI | CACNNNGTG | 1 cut(s) | 326 |
| AfaI | GTAC | 5 cut(s) | 523, 539, 686, 835, 1104 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 596 |
| AflII | CTTAAG | 1 cut(s) | 412 |
| AjnI | CCWGG | 2 cut(s) | 1310, 1357 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 202, 234, 1391, 1423 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 417, 449 |
| AluBI | AGCT | 7 cut(s) | 22, 38, 748, 797, 988, 1198, 1264 |
| AluI | AGCT | 7 cut(s) | 22, 38, 748, 797, 988, 1198, 1264 |
| Alw26I | GTCTC | 5 cut(s) | 170, 297, 498, 1348, 1533 |
| AlwI | GGATC | 3 cut(s) | 183, 969, 1292 |
| AoxI | GGCC | 4 cut(s) | 478, 1127, 1346, 1484 |
| ApeKI | GCWGC | 2 cut(s) | 988, 1195 |
| ApoI | RAATTY | 3 cut(s) | 700, 1256, 1464 |
| AseI | ATTAAT | 2 cut(s) | 564, 708 |
| AspLEI | GCGC | 2 cut(s) | 993, 1162 |
| AspS9I | GGNCC | 3 cut(s) | 479, 587, 872 |
| AsuC2I | CCSGG | 1 cut(s) | 482 |
| AsuHPI | GGTGA | 5 cut(s) | 54, 312, 1013, 1052, 1604 |
| AvaII | GGWCC | 2 cut(s) | 587, 872 |
| AxyI | CCTNAGG | 1 cut(s) | 957 |
| BbvCI | CCTCAGC | 1 cut(s) | 34 |
| BbvI | GCAGC | 2 cut(s) | 975, 1207 |
| BccI | CCATC | 4 cut(s) | 808, 971, 998, 1217 |
| BceAI | ACGGC | 1 cut(s) | 1121 |
| BciT130I | CCWGG | 2 cut(s) | 1312, 1359 |
| BciVI | GTATCC | 1 cut(s) | 1042 |
| BcnI | CCSGG | 1 cut(s) | 482 |
| BcoDI | GTCTC | 5 cut(s) | 170, 297, 498, 1348, 1533 |
| BfaI | CTAG | 3 cut(s) | 296, 324, 365 |
| BfmI | CTRYAG | 2 cut(s) | 1193, 1332 |
| BfrI | CTTAAG | 1 cut(s) | 412 |
| BfuI | GTATCC | 1 cut(s) | 1042 |
| BglII | AGATCT | 1 cut(s) | 1519 |
| BisI | GCNGC | 2 cut(s) | 989, 1196 |
| BlsI | GCNGC | 2 cut(s) | 990, 1197 |
| BmcAI | AGTACT | 2 cut(s) | 523, 539 |
| Bme1390I | CCNGG | 3 cut(s) | 482, 1312, 1359 |
| Bme18I | GGWCC | 2 cut(s) | 587, 872 |
| BmgT120I | GGNCC | 3 cut(s) | 479, 587, 872 |
| BmiI | GGNNCC | 2 cut(s) | 1173, 1506 |
| BmrFI | CCNGG | 3 cut(s) | 482, 1312, 1359 |
| BmrI | ACTGGG | 1 cut(s) | 1262 |
| BmsI | GCATC | 3 cut(s) | 519, 939, 1147 |
| BmuI | ACTGGG | 1 cut(s) | 1262 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 950, 982, 1506, 1538 |
| BpmI | CTGGAG | 2 cut(s) | 393, 1533 |
| Bpu10I | CCTNAGC | 1 cut(s) | 34 |
| BpuMI | CCSGG | 1 cut(s) | 482 |
| BsaAI | YACGTR | 1 cut(s) | 1324 |
| BsaI | GGTCTC | 1 cut(s) | 1348 |
| BsaJI | CCNNGG | 3 cut(s) | 628, 1310, 1534 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 596 |
| Bse1I | ACTGG | 6 cut(s) | 332, 385, 431, 883, 1090, 1268 |
| Bse21I | CCTNAGG | 1 cut(s) | 957 |
| BseBI | CCWGG | 2 cut(s) | 1312, 1359 |
| BseDI | CCNNGG | 3 cut(s) | 628, 1310, 1534 |
| BseGI | GGATG | 1 cut(s) | 699 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 596 |
| BseMII | CTCAG | 5 cut(s) | 48, 525, 948, 1341, 1491 |
| BseNI | ACTGG | 6 cut(s) | 332, 385, 431, 883, 1090, 1268 |
| BseRI | GAGGAG | 1 cut(s) | 1517 |
| BseXI | GCAGC | 2 cut(s) | 975, 1207 |
| BshFI | GGCC | 4 cut(s) | 480, 1129, 1348, 1486 |
| BsiSI | CCGG | 1 cut(s) | 482 |
| BslFI | GGGAC | 2 cut(s) | 236, 614 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 596 |
| BsmAI | GTCTC | 5 cut(s) | 170, 297, 498, 1348, 1533 |
| BsmBI | CGTCTC | 1 cut(s) | 170 |
| BsmFI | GGGAC | 2 cut(s) | 236, 614 |
| BsmI | GAATGC | 1 cut(s) | 432 |
| BsnI | GGCC | 4 cut(s) | 480, 1129, 1348, 1486 |
| Bso31I | GGTCTC | 1 cut(s) | 1348 |
| Bsp143I | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| BspACI | CCGC | 2 cut(s) | 131, 1393 |
| BspANI | GGCC | 4 cut(s) | 480, 1129, 1348, 1486 |
| BspCNI | CTCAG | 5 cut(s) | 47, 526, 949, 1342, 1492 |
| BspLI | GGNNCC | 2 cut(s) | 1173, 1506 |
| BspMAI | CTGCAG | 2 cut(s) | 1197, 1336 |
| BspPI | GGATC | 3 cut(s) | 183, 969, 1292 |
| BspTI | CTTAAG | 1 cut(s) | 412 |
| BspTNI | GGTCTC | 1 cut(s) | 1348 |
| BsrI | ACTGG | 6 cut(s) | 332, 385, 431, 883, 1090, 1268 |
| BssECI | CCNNGG | 3 cut(s) | 628, 1310, 1534 |
| BssMI | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| BssT1I | CCWWGG | 2 cut(s) | 628, 1534 |
| Bst2UI | CCWGG | 2 cut(s) | 1312, 1359 |
| Bst4CI | ACNGT | 7 cut(s) | 361, 917, 1054, 1081, 1102, 1150, 1552 |
| Bst6I | CTCTTC | 1 cut(s) | 450 |
| BstAFI | CTTAAG | 1 cut(s) | 412 |
| BstBAI | YACGTR | 1 cut(s) | 1324 |
| BstC8I | GCNNGC | 2 cut(s) | 1069, 1509 |
| BstDEI | CTNAG | 8 cut(s) | 34, 279, 513, 534, 957, 1350, 1370, 1500 |
| BstF5I | GGATG | 1 cut(s) | 699 |
| BstHHI | GCGC | 2 cut(s) | 993, 1162 |
| BstKTI | GATC | 9 cut(s) | 163, 191, 285, 657, 846, 964, 1300, 1369, 1522 |
| BstMAI | GTCTC | 5 cut(s) | 170, 297, 498, 1348, 1533 |
| BstMBI | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| BstNI | CCWGG | 2 cut(s) | 1312, 1359 |
| BstSCI | CCNGG | 3 cut(s) | 480, 1310, 1357 |
| BstSFI | CTRYAG | 2 cut(s) | 1193, 1332 |
| BstV1I | GCAGC | 2 cut(s) | 975, 1207 |
| BstX2I | RGATCY | 4 cut(s) | 188, 961, 1297, 1519 |
| BstYI | RGATCY | 4 cut(s) | 188, 961, 1297, 1519 |
| Bsu36I | CCTNAGG | 1 cut(s) | 957 |
| BsuI | GTATCC | 1 cut(s) | 1042 |
| BsuRI | GGCC | 4 cut(s) | 480, 1129, 1348, 1486 |
| BtsCI | GGATG | 1 cut(s) | 699 |
| BtsI | GCAGTG | 3 cut(s) | 865, 903, 1190 |
| BtsIMutI | CAGTG | 6 cut(s) | 357, 865, 903, 1077, 1097, 1190 |
| Cac8I | GCNNGC | 2 cut(s) | 1069, 1509 |
| CfoI | GCGC | 2 cut(s) | 993, 1162 |
| Cfr13I | GGNCC | 3 cut(s) | 479, 587, 872 |
| Csp6I | GTAC | 5 cut(s) | 522, 538, 685, 834, 1103 |
| CviQI | GTAC | 5 cut(s) | 522, 538, 685, 834, 1103 |
| DdeI | CTNAG | 8 cut(s) | 34, 279, 513, 534, 957, 1350, 1370, 1500 |
| DpnI | GATC | 9 cut(s) | 162, 190, 284, 656, 845, 963, 1299, 1368, 1521 |
| DpnII | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| DraI | TTTAAA | 1 cut(s) | 889 |
| DraIII | CACNNNGTG | 1 cut(s) | 326 |
| Eam1104I | CTCTTC | 1 cut(s) | 450 |
| EarI | CTCTTC | 1 cut(s) | 450 |
| Eco130I | CCWWGG | 2 cut(s) | 628, 1534 |
| Eco147I | AGGCCT | 2 cut(s) | 1129, 1486 |
| Eco31I | GGTCTC | 1 cut(s) | 1348 |
| Eco47I | GGWCC | 2 cut(s) | 587, 872 |
| Eco57I | CTGAAG | 1 cut(s) | 32 |
| Eco81I | CCTNAGG | 1 cut(s) | 957 |
| EcoRI | GAATTC | 1 cut(s) | 1464 |
| EcoRII | CCWGG | 2 cut(s) | 1310, 1357 |
| EcoT14I | CCWWGG | 2 cut(s) | 628, 1534 |
| ErhI | CCWWGG | 2 cut(s) | 628, 1534 |
| Esp3I | CGTCTC | 1 cut(s) | 170 |
| FaqI | GGGAC | 2 cut(s) | 236, 614 |
| FauNDI | CATATG | 1 cut(s) | 973 |
| Fnu4HI | GCNGC | 2 cut(s) | 989, 1196 |
| FokI | GGATG | 1 cut(s) | 706 |
| Fsp4HI | GCNGC | 2 cut(s) | 989, 1196 |
| FspBI | CTAG | 3 cut(s) | 296, 324, 365 |
| GlaI | GCGC | 2 cut(s) | 992, 1161 |
| GluI | GCNGC | 2 cut(s) | 989, 1196 |
| GsuI | CTGGAG | 2 cut(s) | 393, 1533 |
| HaeIII | GGCC | 4 cut(s) | 480, 1129, 1348, 1486 |
| HapII | CCGG | 1 cut(s) | 482 |
| HhaI | GCGC | 2 cut(s) | 993, 1162 |
| Hin6I | GCGC | 2 cut(s) | 991, 1160 |
| HinP1I | GCGC | 2 cut(s) | 991, 1160 |
| HincII | GTYRAC | 1 cut(s) | 406 |
| HindII | GTYRAC | 1 cut(s) | 406 |
| HinfI | GANTC | 5 cut(s) | 234, 290, 549, 665, 943 |
| HpaII | CCGG | 1 cut(s) | 482 |
| HphI | GGTGA | 5 cut(s) | 54, 312, 1013, 1052, 1604 |
| Hpy166II | GTNNAC | 7 cut(s) | 329, 406, 872, 924, 1321, 1376, 1595 |
| Hpy188I | TCNGA | 7 cut(s) | 160, 174, 843, 935, 1032, 1156, 1293 |
| Hpy188III | TCNNGA | 8 cut(s) | 50, 207, 238, 553, 608, 1420, 1454, 1573 |
| Hpy8I | GTNNAC | 7 cut(s) | 329, 406, 872, 924, 1321, 1376, 1595 |
| Hpy99I | CGWCG | 1 cut(s) | 1014 |
| HpyAV | CCTTC | 7 cut(s) | 102, 148, 390, 590, 758, 1244, 1429 |
| HpyCH4III | ACNGT | 7 cut(s) | 361, 917, 1054, 1081, 1102, 1150, 1552 |
| HpyCH4IV | ACGT | 3 cut(s) | 179, 245, 1323 |
| HpyCH4V | TGCA | 6 cut(s) | 908, 971, 1142, 1195, 1334, 1493 |
| HpyF3I | CTNAG | 8 cut(s) | 34, 279, 513, 534, 957, 1350, 1370, 1500 |
| HpySE526I | ACGT | 3 cut(s) | 179, 245, 1323 |
| HspAI | GCGC | 2 cut(s) | 991, 1160 |
| Kzo9I | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| LmnI | GCTCC | 6 cut(s) | 374, 794, 1171, 1269, 1504, 1514 |
| Lsp1109I | GCAGC | 2 cut(s) | 975, 1207 |
| LweI | GCATC | 3 cut(s) | 519, 939, 1147 |
| MaeI | CTAG | 3 cut(s) | 296, 324, 365 |
| MaeII | ACGT | 3 cut(s) | 179, 245, 1323 |
| MaeIII | GTNAC | 4 cut(s) | 318, 1058, 1188, 1339 |
| MalI | GATC | 9 cut(s) | 162, 190, 284, 656, 845, 963, 1299, 1368, 1521 |
| MboI | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| MboII | GAAGA | 8 cut(s) | 21, 38, 104, 244, 247, 430, 467, 1110 |
| MfeI | CAATTG | 1 cut(s) | 1476 |
| MflI | RGATCY | 4 cut(s) | 188, 961, 1297, 1519 |
| MlyI | GAGTC | 2 cut(s) | 299, 558 |
| MmeI | TCCRAC | 3 cut(s) | 336, 481, 709 |
| MslI | CAYNNNNRTG | 1 cut(s) | 692 |
| MspCI | CTTAAG | 1 cut(s) | 412 |
| MspI | CCGG | 1 cut(s) | 482 |
| MspR9I | CCNGG | 3 cut(s) | 482, 1312, 1359 |
| MunI | CAATTG | 1 cut(s) | 1476 |
| Mva1269I | GAATGC | 1 cut(s) | 432 |
| MvaI | CCWGG | 2 cut(s) | 1312, 1359 |
| NciI | CCSGG | 1 cut(s) | 482 |
| NdeI | CATATG | 1 cut(s) | 973 |
| NdeII | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| NlaIV | GGNNCC | 2 cut(s) | 1173, 1506 |
| NmeAIII | GCCGAG | 2 cut(s) | 1200, 1324 |
| NmuCI | GTSAC | 2 cut(s) | 318, 1188 |
| PceI | AGGCCT | 2 cut(s) | 1129, 1486 |
| PctI | GAATGC | 1 cut(s) | 432 |
| PfeI | GAWTC | 3 cut(s) | 234, 665, 943 |
| PkrI | GCNGC | 2 cut(s) | 990, 1197 |
| PleI | GAGTC | 2 cut(s) | 298, 557 |
| PpsI | GAGTC | 2 cut(s) | 298, 557 |
| Ppu21I | YACGTR | 1 cut(s) | 1324 |
| PshBI | ATTAAT | 2 cut(s) | 564, 708 |
| PsiI | TTATAA | 1 cut(s) | 1017 |
| Psp1406I | AACGTT | 1 cut(s) | 245 |
| Psp6I | CCWGG | 2 cut(s) | 1310, 1357 |
| PspGI | CCWGG | 2 cut(s) | 1310, 1357 |
| PspN4I | GGNNCC | 2 cut(s) | 1173, 1506 |
| PspPI | GGNCC | 3 cut(s) | 479, 587, 872 |
| PstI | CTGCAG | 2 cut(s) | 1197, 1336 |
| PsuI | RGATCY | 4 cut(s) | 188, 961, 1297, 1519 |
| RsaI | GTAC | 5 cut(s) | 523, 539, 686, 835, 1104 |
| RsaNI | GTAC | 5 cut(s) | 522, 538, 685, 834, 1103 |
| RseI | CAYNNNNRTG | 1 cut(s) | 692 |
| SatI | GCNGC | 2 cut(s) | 989, 1196 |
| Sau3AI | GATC | 9 cut(s) | 160, 188, 282, 654, 843, 961, 1297, 1366, 1519 |
| Sau96I | GGNCC | 3 cut(s) | 479, 587, 872 |
| ScaI | AGTACT | 2 cut(s) | 523, 539 |
| SchI | GAGTC | 2 cut(s) | 299, 558 |
| ScrFI | CCNGG | 3 cut(s) | 482, 1312, 1359 |
| SfaNI | GCATC | 3 cut(s) | 519, 939, 1147 |
| SfcI | CTRYAG | 2 cut(s) | 1193, 1332 |
| SinI | GGWCC | 2 cut(s) | 587, 872 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 692 |
| SmlI | CTYRAG | 1 cut(s) | 412 |
| SmoI | CTYRAG | 1 cut(s) | 412 |
| SseBI | AGGCCT | 2 cut(s) | 1129, 1486 |
| SsiI | CCGC | 2 cut(s) | 131, 1393 |
| SspI | AATATT | 1 cut(s) | 1183 |
| SspMI | CTAG | 3 cut(s) | 296, 324, 365 |
| StuI | AGGCCT | 2 cut(s) | 1129, 1486 |
| StyD4I | CCNGG | 3 cut(s) | 480, 1310, 1357 |
| StyI | CCWWGG | 2 cut(s) | 628, 1534 |
| TaaI | ACNGT | 7 cut(s) | 361, 917, 1054, 1081, 1102, 1150, 1552 |
| TaiI | ACGT | 3 cut(s) | 182, 248, 1326 |
| TaqI | TCGA | 3 cut(s) | 394, 436, 846 |
| TatI | WGTACW | 4 cut(s) | 521, 537, 684, 833 |
| TfiI | GAWTC | 3 cut(s) | 234, 665, 943 |
| TscAI | CASTG | 6 cut(s) | 364, 865, 910, 1084, 1097, 1197 |
| TseFI | GTSAC | 2 cut(s) | 318, 1188 |
| TseI | GCWGC | 2 cut(s) | 988, 1195 |
| Tsp45I | GTSAC | 2 cut(s) | 318, 1188 |
| TspDTI | ATGAA | 6 cut(s) | 48, 276, 468, 710, 777, 1590 |
| TspRI | CASTG | 6 cut(s) | 364, 865, 910, 1084, 1097, 1197 |
| Vha464I | CTTAAG | 1 cut(s) | 412 |
| VpaK11BI | GGWCC | 2 cut(s) | 587, 872 |
| VspI | ATTAAT | 2 cut(s) | 564, 708 |
| XapI | RAATTY | 3 cut(s) | 700, 1256, 1464 |
| XspI | CTAG | 3 cut(s) | 296, 324, 365 |
| ZrmI | AGTACT | 2 cut(s) | 523, 539 |