Rroxscaffold_1G00064860

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000908
Physical Location & Seq
Forward (+)
86696118 .. 86701489
5372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00064860.1

Sequence Viewer

Length: 1659 bp
ATGGAACTTGGTGTCATTAAGCTCTCATCTTCCTCAGCTCTCAATCTTCAGGATTTTTCATCACCTGTTGGTAGGAAACTTATTAACAAACCTTCACTTCCTAAACCATTTCTTCAACTTAATGGCAGACCACAGACCTTCCTCAACAAACTAAAATCTGATCGTCTCCCTCCAAGAACATCGAAAAGATCCATATTAGCACAATCTGGAAGTCAGGGTTGGGACTTGGGGAGATTCTTGAAAACGTTATTCTTCTTCAATGAACCCCGCTCTCCTGCTAAGATCATTGAGTCTCTAGTCAGCAAACTATCCAACCCGTCACCTAGTGAACCAGTAAAGAGAATGGAAACTTCTGGCACTGTCCTAGTGGCTGGAGCAACTGGTGGTGTAGGTCGAAGGGTGGTCAACATCTTAAGGAAGAAAGGAATGCCAGTTCGAGTATTGGTTAGAAATGAAGAGAAAGCAAGACAGATGTTAGGCCCGGACATTGACTTGATTGTTGGAGACATTACTAAGGAGAGTACTTTAATCCCCGAGTACTTCAAAGGAGTCAGGAGAGTGATTAATGCTGTTTCTGTCATTGTTGGTCCAAAGGAAGGGGACACTCCTGACAGGCAAAAATACAGCCAAATCAAGGGAGATTCGCCTGAAAAGGTGGAGTACATTGGGATGAAAAATTTGATTAATGCTCTAAAACAAAGTGTTGGACTTCAAAATGGAAAGCTACTATTTGGATATGAAGGTAATAATTATAGGGAATTGACTTGGGGAGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCTGATCGACCCAAATGGCAGTGAAAATGGTGGACCAACTGGACTTTTTAAAGGAGTTGTTTCCACTGCAAATAACGGTGGGTTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTGCGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACACTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTGGGTTCTGCAGGAGTTACCCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATACCATTTAGCGAGCCATTTACAGTAGACCCCGAAAATCCACCCCCTGAGAAGGACTACGATGTCTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCCTGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

552

Amino Acids

60.72

Weight (kDa)

8.98

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 118 - 192 3.1e-09 NmrA-like family
NAD_binding_10 PF13460 125 - 233 6.4e-11 NAD(P)H-binding
CIA30 PF08547 254 - 363 1.9e-29 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 397 - 496 9.2e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
AasI GACNNNNNNGTC 1 cut(s) 1586
AccBSI CCGCTC 1 cut(s) 270
AccI GTMKAC 2 cut(s) 1551, 1590
AciI CCGC 2 cut(s) 268, 1273
AclI AACGTT 1 cut(s) 245
AclWI GGATC 3 cut(s) 183, 945, 1172
AcsI RAATTY 4 cut(s) 676, 1136, 1344, 1461
AcuI CTGAAG 1 cut(s) 32
AcvI CACGTG 1 cut(s) 1204
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 6 cut(s) 523, 539, 662, 811, 1200, 1650
AfiI CCNNNNNNNGG 3 cut(s) 596, 634, 1576
AflII CTTAAG 1 cut(s) 412
AflIII ACRYGT 1 cut(s) 1201
AjnI CCWGG 2 cut(s) 1190, 1237
AjuI GAANNNNNNNTTGG 4 cut(s) 202, 234, 1271, 1303
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 6 cut(s) 22, 38, 724, 773, 964, 1144
AluI AGCT 6 cut(s) 22, 38, 724, 773, 964, 1144
Alw26I GTCTC 5 cut(s) 170, 297, 498, 1228, 1413
AlwI GGATC 3 cut(s) 183, 945, 1172
Ama87I CYCGRG 1 cut(s) 533
AoxI GGCC 3 cut(s) 478, 1226, 1364
ApeKI GCWGC 2 cut(s) 964, 1472
ApoI RAATTY 4 cut(s) 676, 1136, 1344, 1461
AseI ATTAAT 2 cut(s) 564, 684
AspLEI GCGC 1 cut(s) 969
AspS9I GGNCC 3 cut(s) 479, 587, 848
AsuC2I CCSGG 2 cut(s) 482, 1225
AsuHPI GGTGA 4 cut(s) 54, 312, 989, 1028
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 587, 848
AxyI CCTNAGG 1 cut(s) 933
BbrPI CACGTG 1 cut(s) 1204
BbvCI CCTCAGC 1 cut(s) 34
BbvI GCAGC 2 cut(s) 951, 1459
BccI CCATC 5 cut(s) 784, 947, 974, 1097, 1604
BciT130I CCWGG 2 cut(s) 1192, 1239
BciVI GTATCC 1 cut(s) 1018
BcnI CCSGG 2 cut(s) 482, 1225
BcoDI GTCTC 5 cut(s) 170, 297, 498, 1228, 1413
BfaI CTAG 3 cut(s) 296, 324, 365
BfmI CTRYAG 1 cut(s) 1212
BfrI CTTAAG 1 cut(s) 412
BfuI GTATCC 1 cut(s) 1018
BglII AGATCT 1 cut(s) 1399
BisI GCNGC 2 cut(s) 965, 1473
BlsI GCNGC 2 cut(s) 966, 1474
BmcAI AGTACT 2 cut(s) 523, 539
Bme1390I CCNGG 4 cut(s) 482, 1192, 1225, 1239
Bme18I GGWCC 2 cut(s) 587, 848
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 3 cut(s) 479, 587, 848
BmiI GGNNCC 1 cut(s) 1386
BmrFI CCNGG 4 cut(s) 482, 1192, 1225, 1239
BmrI ACTGGG 1 cut(s) 1142
BmsI GCATC 1 cut(s) 915
BmuI ACTGGG 1 cut(s) 1142
BplI GAGNNNNNCTC 4 cut(s) 926, 958, 1386, 1418
BpmI CTGGAG 2 cut(s) 393, 1413
Bpu10I CCTNAGC 1 cut(s) 34
BpuMI CCSGG 2 cut(s) 482, 1225
BsaAI YACGTR 1 cut(s) 1204
BsaI GGTCTC 1 cut(s) 1228
BsaJI CCNNGG 2 cut(s) 1190, 1414
Bsc4I CCNNNNNNNGG 3 cut(s) 596, 634, 1576
Bse1I ACTGG 8 cut(s) 332, 385, 431, 859, 1066, 1148, 1501, 1652
Bse21I CCTNAGG 1 cut(s) 933
BseBI CCWGG 2 cut(s) 1192, 1239
BseDI CCNNGG 2 cut(s) 1190, 1414
BseGI GGATG 2 cut(s) 675, 1615
BseLI CCNNNNNNNGG 3 cut(s) 596, 634, 1576
BseMII CTCAG 5 cut(s) 48, 924, 1221, 1371, 1563
BseNI ACTGG 8 cut(s) 332, 385, 431, 859, 1066, 1148, 1501, 1652
BseRI GAGGAG 1 cut(s) 1397
BseXI GCAGC 2 cut(s) 951, 1459
BshFI GGCC 3 cut(s) 480, 1228, 1366
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 2 cut(s) 482, 1225
BslFI GGGAC 2 cut(s) 236, 614
BslI CCNNNNNNNGG 3 cut(s) 596, 634, 1576
BsmAI GTCTC 5 cut(s) 170, 297, 498, 1228, 1413
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 236, 614
BsmI GAATGC 1 cut(s) 432
BsnI GGCC 3 cut(s) 480, 1228, 1366
Bso31I GGTCTC 1 cut(s) 1228
BsoBI CYCGRG 1 cut(s) 533
Bsp1407I TGTACA 1 cut(s) 1198
Bsp143I GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
BspACI CCGC 2 cut(s) 268, 1273
BspANI GGCC 3 cut(s) 480, 1228, 1366
BspCNI CTCAG 5 cut(s) 47, 925, 1222, 1372, 1564
BspLI GGNNCC 1 cut(s) 1386
BspMAI CTGCAG 1 cut(s) 1216
BspPI GGATC 3 cut(s) 183, 945, 1172
BspTI CTTAAG 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 1228
BsrBI CCGCTC 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 1198
BsrI ACTGG 8 cut(s) 332, 385, 431, 859, 1066, 1148, 1501, 1652
BssECI CCNNGG 2 cut(s) 1190, 1414
BssMI GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
BssT1I CCWWGG 1 cut(s) 1414
Bst2UI CCWGG 2 cut(s) 1192, 1239
Bst4CI ACNGT 5 cut(s) 361, 893, 1030, 1057, 1549
Bst6I CTCTTC 2 cut(s) 450, 1443
BstAFI CTTAAG 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 1198
BstBAI YACGTR 1 cut(s) 1204
BstC8I GCNNGC 3 cut(s) 1045, 1389, 1538
BstDEI CTNAG 9 cut(s) 34, 279, 513, 933, 1230, 1250, 1380, 1572, 1633
BstF5I GGATG 2 cut(s) 675, 1615
BstHHI GCGC 1 cut(s) 969
BstKTI GATC 8 cut(s) 163, 191, 285, 822, 940, 1180, 1249, 1402
BstMAI GTCTC 5 cut(s) 170, 297, 498, 1228, 1413
BstMBI GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
BstNI CCWGG 2 cut(s) 1192, 1239
BstSCI CCNGG 4 cut(s) 480, 1190, 1223, 1237
BstSFI CTRYAG 1 cut(s) 1212
BstV1I GCAGC 2 cut(s) 951, 1459
BstX2I RGATCY 4 cut(s) 188, 937, 1177, 1399
BstYI RGATCY 4 cut(s) 188, 937, 1177, 1399
Bsu36I CCTNAGG 1 cut(s) 933
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 3 cut(s) 480, 1228, 1366
BtsCI GGATG 2 cut(s) 675, 1615
BtsI GCAGTG 2 cut(s) 841, 879
BtsIMutI CAGTG 5 cut(s) 357, 841, 879, 1053, 1073
Cac8I GCNNGC 3 cut(s) 1045, 1389, 1538
CfoI GCGC 1 cut(s) 969
Cfr13I GGNCC 3 cut(s) 479, 587, 848
Csp6I GTAC 6 cut(s) 522, 538, 661, 810, 1199, 1649
CviQI GTAC 6 cut(s) 522, 538, 661, 810, 1199, 1649
DdeI CTNAG 9 cut(s) 34, 279, 513, 933, 1230, 1250, 1380, 1572, 1633
DpnI GATC 8 cut(s) 162, 190, 284, 821, 939, 1179, 1248, 1401
DpnII GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
DraI TTTAAA 2 cut(s) 865, 1597
DraIII CACNNNGTG 1 cut(s) 326
DrdI GACNNNNNNGTC 1 cut(s) 1586
DseDI GACNNNNNNGTC 1 cut(s) 1586
Eam1104I CTCTTC 2 cut(s) 450, 1443
EarI CTCTTC 2 cut(s) 450, 1443
Eco130I CCWWGG 1 cut(s) 1414
Eco147I AGGCCT 1 cut(s) 1366
Eco31I GGTCTC 1 cut(s) 1228
Eco32I GATATC 1 cut(s) 1440
Eco47I GGWCC 2 cut(s) 587, 848
Eco57I CTGAAG 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 1204
Eco81I CCTNAGG 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 533
EcoRI GAATTC 1 cut(s) 1344
EcoRII CCWGG 2 cut(s) 1190, 1237
EcoRV GATATC 1 cut(s) 1440
EcoT14I CCWWGG 1 cut(s) 1414
ErhI CCWWGG 1 cut(s) 1414
Esp3I CGTCTC 1 cut(s) 170
FaqI GGGAC 2 cut(s) 236, 614
FauI CCCGC 1 cut(s) 275
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 2 cut(s) 1551, 1590
Fnu4HI GCNGC 2 cut(s) 965, 1473
FokI GGATG 2 cut(s) 682, 1622
Fsp4HI GCNGC 2 cut(s) 965, 1473
FspBI CTAG 3 cut(s) 296, 324, 365
GlaI GCGC 1 cut(s) 968
GluI GCNGC 2 cut(s) 965, 1473
GsuI CTGGAG 2 cut(s) 393, 1413
HaeIII GGCC 3 cut(s) 480, 1228, 1366
HapII CCGG 2 cut(s) 482, 1225
HhaI GCGC 1 cut(s) 969
Hin6I GCGC 1 cut(s) 967
HinP1I GCGC 1 cut(s) 967
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HinfI GANTC 5 cut(s) 234, 290, 549, 641, 919
HpaII CCGG 2 cut(s) 482, 1225
HphI GGTGA 4 cut(s) 54, 312, 989, 1028
Hpy166II GTNNAC 8 cut(s) 329, 406, 848, 900, 1201, 1256, 1552, 1591
Hpy188I TCNGA 5 cut(s) 160, 819, 911, 1008, 1173
Hpy188III TCNNGA 8 cut(s) 50, 207, 238, 553, 608, 1300, 1334, 1443
Hpy8I GTNNAC 8 cut(s) 329, 406, 848, 900, 1201, 1256, 1552, 1591
Hpy99I CGWCG 1 cut(s) 990
HpyAV CCTTC 9 cut(s) 102, 148, 390, 590, 734, 1124, 1309, 1570, 1600
HpyCH4III ACNGT 5 cut(s) 361, 893, 1030, 1057, 1549
HpyCH4IV ACGT 2 cut(s) 245, 1203
HpyCH4V TGCA 5 cut(s) 884, 947, 1214, 1373, 1475
HpyF3I CTNAG 9 cut(s) 34, 279, 513, 933, 1230, 1250, 1380, 1572, 1633
HpySE526I ACGT 2 cut(s) 245, 1203
HspAI GCGC 1 cut(s) 967
Kzo9I GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
LmnI GCTCC 5 cut(s) 374, 770, 1149, 1384, 1394
Lsp1109I GCAGC 2 cut(s) 951, 1459
LweI GCATC 1 cut(s) 915
MaeI CTAG 3 cut(s) 296, 324, 365
MaeII ACGT 2 cut(s) 245, 1203
MaeIII GTNAC 3 cut(s) 318, 1034, 1219
MalI GATC 8 cut(s) 162, 190, 284, 821, 939, 1179, 1248, 1401
MbiI CCGCTC 1 cut(s) 270
MboI GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
MboII GAAGA 8 cut(s) 21, 38, 104, 244, 247, 430, 467, 1460
MfeI CAATTG 1 cut(s) 1356
MflI RGATCY 4 cut(s) 188, 937, 1177, 1399
MlyI GAGTC 2 cut(s) 299, 558
MmeI TCCRAC 3 cut(s) 336, 481, 685
MslI CAYNNNNRTG 1 cut(s) 668
MspCI CTTAAG 1 cut(s) 412
MspI CCGG 2 cut(s) 482, 1225
MspR9I CCNGG 4 cut(s) 482, 1192, 1225, 1239
MunI CAATTG 1 cut(s) 1356
Mva1269I GAATGC 1 cut(s) 432
MvaI CCWGG 2 cut(s) 1192, 1239
NciI CCSGG 2 cut(s) 482, 1225
NdeI CATATG 1 cut(s) 949
NdeII GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
NlaIV GGNNCC 1 cut(s) 1386
NmuCI GTSAC 1 cut(s) 318
PceI AGGCCT 1 cut(s) 1366
PctI GAATGC 1 cut(s) 432
PfeI GAWTC 3 cut(s) 234, 641, 919
PkrI GCNGC 2 cut(s) 966, 1474
PleI GAGTC 2 cut(s) 298, 557
PmaCI CACGTG 1 cut(s) 1204
PmlI CACGTG 1 cut(s) 1204
PpsI GAGTC 2 cut(s) 298, 557
Ppu21I YACGTR 1 cut(s) 1204
PshBI ATTAAT 2 cut(s) 564, 684
PsiI TTATAA 1 cut(s) 993
Psp1406I AACGTT 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 1190, 1237
PspCI CACGTG 1 cut(s) 1204
PspGI CCWGG 2 cut(s) 1190, 1237
PspN4I GGNNCC 1 cut(s) 1386
PspPI GGNCC 3 cut(s) 479, 587, 848
PstI CTGCAG 1 cut(s) 1216
PsuI RGATCY 4 cut(s) 188, 937, 1177, 1399
RsaI GTAC 6 cut(s) 523, 539, 662, 811, 1200, 1650
RsaNI GTAC 6 cut(s) 522, 538, 661, 810, 1199, 1649
RseI CAYNNNNRTG 1 cut(s) 668
SatI GCNGC 2 cut(s) 965, 1473
Sau3AI GATC 8 cut(s) 160, 188, 282, 819, 937, 1177, 1246, 1399
Sau96I GGNCC 3 cut(s) 479, 587, 848
ScaI AGTACT 2 cut(s) 523, 539
SchI GAGTC 2 cut(s) 299, 558
ScrFI CCNGG 4 cut(s) 482, 1192, 1225, 1239
SfaNI GCATC 1 cut(s) 915
SfcI CTRYAG 1 cut(s) 1212
SinI GGWCC 2 cut(s) 587, 848
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
SseBI AGGCCT 1 cut(s) 1366
SsiI CCGC 2 cut(s) 268, 1273
SspMI CTAG 3 cut(s) 296, 324, 365
StuI AGGCCT 1 cut(s) 1366
StyD4I CCNGG 4 cut(s) 480, 1190, 1223, 1237
StyI CCWWGG 1 cut(s) 1414
TaaI ACNGT 5 cut(s) 361, 893, 1030, 1057, 1549
TaiI ACGT 2 cut(s) 248, 1206
TaqI TCGA 4 cut(s) 182, 394, 436, 822
TatI WGTACW 5 cut(s) 521, 537, 660, 809, 1198
TfiI GAWTC 3 cut(s) 234, 641, 919
TscAI CASTG 5 cut(s) 364, 841, 886, 1060, 1073
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 2 cut(s) 964, 1472
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 5 cut(s) 48, 276, 468, 686, 753
TspRI CASTG 5 cut(s) 364, 841, 886, 1060, 1073
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 2 cut(s) 587, 848
VspI ATTAAT 2 cut(s) 564, 684
XapI RAATTY 4 cut(s) 676, 1136, 1344, 1461
XmiI GTMKAC 2 cut(s) 1551, 1590
XspI CTAG 3 cut(s) 296, 324, 365
ZrmI AGTACT 2 cut(s) 523, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.