Rorug02G0325700

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000908
Physical Location & Seq
Reverse (-)
39970482 .. 39972701
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0325700.1

Sequence Viewer

Length: 1347 bp
ATGACTTCACCAACACAGAAACAATCATCGAGAGACAAGACATCCAAGAAAAAAAAATCTAAAGCCAAGAATGATGAGGTGCTTCAGCCCATGGAGGACTTTCAATCAAAGGAGACTATCAACACCTCACCTTTGGTCAGAGCGAAGCGTGGTGTCACAGAAATGCATAAGGTTGTTGTGAAAAAAGCTTTGGGTAAAAGATATCAAGTTCAGTACACTAGACTTGGTGTACCAAGAGGGAGAGTACGTTCGATGCTGCAGTCTTACATTGGAGTTTTGGCACGAACTATGGTTCCTATAAACATCCCAAACTGGCCAAACGTTCCACGGGATATAAAAGAAAAAATATGGGAAGAGATTGAGGGTGCTTTCATTATGGCTCCTGAAAGTAAGAAGCTGGTGTTGTCATCTGCTGCCTCTAAATGGAGACAATTCAAGAGTAAGTTAACAAGTAAGTATGTATTACCTTATGCTGGGAGGAAAAAGAAACTGAAGAAACCACCAAAGATGTATGATTCCATCGAGAGAGATGAATGGAAACAGTTTGTTGCTTCACGATTATCAAATGATTGGCTGATGGAGGCCATGTCTGAAGAAGAAGAACTTGATCGTTCTTATTTATGGAAGAAAGCACGGGAAGGTAAAAATGGAGAAATGGCAGAGGAGGTAGCTCCGGTGGCCAATGATATTGATAATTTGTTGGCTAAGAAAAATAAAGGCGAGCTCGCCATCTCGGGGTCTAATGATGTTCTAACAATGGCGCTAAAAACTCCGGAACATTCTGGTAGAGTAAGGGGTGTTGGGGAACGTGAAAAAGAGCGAGCATATTTTGAGAAAAAGATTGCTGCATTGGAGGCAAAACTGAATGGCAATGTCCTTATTACACCTTCTCCTCCCATTTTTGATAAAGGAAGTAGCCATAGTAAAACTATGTTCAATGGTAATGATGCTTTAAAATCATCTCAATCTACCTATGATATTGAAATTGAGGAAGTGGAGGACATCGAGGTACCAAAAAAGCCAGGCAATATGAAGAATAAATCATGCAAGCTGGCAGCGGGTTCCATTCACAATATTGTGGCTTTAGGCACAATAATTGAAGAGAATGACTCTAATATTACTGTACATGGTATTCCTTTACAAGAGGGTCATTCGAGGGTTGCTGTTGATGTGTCCATTGATGCAAATGCTTTGCTTCCTGTCCCAATTCCAGATGAATTGGAGAATGTTGGTCAAGCTGATGGGAGCTATATATGTGGCTTGGCCAAAGAAGCTCATTGTGTTGAATATGGAAAAGCAAAAAAAAAAGTATGTTGCTCCGGTAAAAGACACATTCATGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

448

Amino Acids

50.08

Weight (kDa)

9.33

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8039 PF26133 346 - 418 5.1e-09 Domain of unknown function (DUF8039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 1009
AccIII TCCGGA 1 cut(s) 772
AciI CCGC 1 cut(s) 1058
AclI AACGTT 1 cut(s) 321
AcoI YGGCCR 3 cut(s) 314, 678, 1263
AcuI CTGAAG 3 cut(s) 68, 512, 612
AfaI GTAC 5 cut(s) 215, 231, 246, 1011, 1125
AfiI CCNNNNNNNGG 3 cut(s) 423, 473, 735
AgsI TTSAA 6 cut(s) 104, 436, 937, 983, 1100, 1286
AjnI CCWGG 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AloI GAACNNNNNNTCC 4 cut(s) 232, 264, 277, 309
AluBI AGCT 8 cut(s) 188, 397, 671, 724, 1051, 1238, 1248, 1274
AluI AGCT 8 cut(s) 188, 397, 671, 724, 1051, 1238, 1248, 1274
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 3 cut(s) 27, 107, 421
Ama87I CYCGRG 1 cut(s) 733
Aor13HI TCCGGA 1 cut(s) 772
AoxI GGCC 4 cut(s) 314, 582, 678, 1263
ApeKI GCWGC 4 cut(s) 256, 413, 845, 1055
Asp718I GGTACC 1 cut(s) 1009
AspLEI GCGC 1 cut(s) 763
AsuHPI GGTGA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 733
BalI TGGCCA 3 cut(s) 316, 680, 1265
BanI GGYRCC 1 cut(s) 1009
BanII GRGCYC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 4 cut(s) 243, 400, 832, 1067
BccI CCATC 4 cut(s) 527, 571, 737, 1235
BciT130I CCWGG 1 cut(s) 1023
BcoDI GTCTC 3 cut(s) 27, 107, 421
BfaI CTAG 1 cut(s) 219
BfmI CTRYAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 764
BisI GCNGC 4 cut(s) 257, 414, 846, 1056
BlsI GCNGC 4 cut(s) 258, 415, 847, 1057
Bme1390I CCNGG 1 cut(s) 1023
BmeT110I CYCGRG 1 cut(s) 733
BmiI GGNNCC 4 cut(s) 294, 381, 1011, 1063
BmrFI CCNGG 1 cut(s) 1023
BmsI GCATC 3 cut(s) 243, 937, 1171
BplI GAGNNNNNCTC 2 cut(s) 1094, 1126
BsaJI CCNNGG 2 cut(s) 90, 326
BsaWI WCCGGW 3 cut(s) 673, 772, 1319
BsaXI ACNNNNNCTCC 6 cut(s) 232, 262, 572, 602, 874, 904
Bsc4I CCNNNNNNNGG 3 cut(s) 423, 473, 735
Bse1I ACTGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 877
BseAI TCCGGA 1 cut(s) 772
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 2 cut(s) 90, 326
BseGI GGATG 2 cut(s) 41, 303
BseLI CCNNNNNNNGG 3 cut(s) 423, 473, 735
BseMI GCAATG 1 cut(s) 877
BseNI ACTGG 1 cut(s) 317
BseRI GAGGAG 2 cut(s) 677, 882
BseXI GCAGC 4 cut(s) 243, 400, 832, 1067
BseYI CCCAGC 1 cut(s) 473
BshFI GGCC 4 cut(s) 316, 584, 680, 1265
BshNI GGYRCC 1 cut(s) 1009
BsiHKAI GWGCWC 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 3 cut(s) 674, 773, 1320
BslFI GGGAC 1 cut(s) 1187
BslI CCNNNNNNNGG 3 cut(s) 423, 473, 735
BsmAI GTCTC 3 cut(s) 27, 107, 421
BsmFI GGGAC 1 cut(s) 1187
BsnI GGCC 4 cut(s) 316, 584, 680, 1265
BsoBI CYCGRG 1 cut(s) 733
Bsp1286I GDGCHC 1 cut(s) 726
Bsp13I TCCGGA 1 cut(s) 772
Bsp1407I TGTACA 1 cut(s) 1123
Bsp143I GATC 1 cut(s) 607
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 4 cut(s) 316, 584, 680, 1265
BspEI TCCGGA 1 cut(s) 772
BspHI TCATGA 1 cut(s) 1336
BspLI GGNNCC 4 cut(s) 294, 381, 1011, 1063
BspMAI CTGCAG 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 1009
BsrDI GCAATG 1 cut(s) 877
BsrGI TGTACA 1 cut(s) 1123
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 90, 326
BssMI GATC 1 cut(s) 607
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 1023
Bst4CI ACNGT 2 cut(s) 543, 1123
Bst6I CTCTTC 2 cut(s) 348, 1095
BstAUI TGTACA 1 cut(s) 1123
BstC8I GCNNGC 5 cut(s) 722, 726, 822, 1049, 1053
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 2 cut(s) 90, 326
BstF5I GGATG 2 cut(s) 41, 303
BstH2I RGCGCY 1 cut(s) 764
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 1 cut(s) 610
BstMAI GTCTC 3 cut(s) 27, 107, 421
BstMBI GATC 1 cut(s) 607
BstMWI GCNNNNNNNGC 3 cut(s) 677, 854, 1271
BstNI CCWGG 1 cut(s) 1023
BstSCI CCNGG 1 cut(s) 1021
BstSFI CTRYAG 1 cut(s) 257
BstV1I GCAGC 4 cut(s) 243, 400, 832, 1067
BsuRI GGCC 4 cut(s) 316, 584, 680, 1265
BtgI CCRYGG 2 cut(s) 90, 326
BtsCI GGATG 2 cut(s) 41, 303
Cac8I GCNNGC 5 cut(s) 722, 726, 822, 1049, 1053
CciI TCATGA 1 cut(s) 1336
CfoI GCGC 1 cut(s) 763
Csp6I GTAC 5 cut(s) 214, 230, 245, 1010, 1124
CviAII CATG 6 cut(s) 91, 586, 1044, 1127, 1337, 1344
CviQI GTAC 5 cut(s) 214, 230, 245, 1010, 1124
DdeI CTNAG 1 cut(s) 705
DpnI GATC 1 cut(s) 609
DpnII GATC 1 cut(s) 607
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 3 cut(s) 314, 678, 1263
Eam1104I CTCTTC 2 cut(s) 348, 1095
EarI CTCTTC 2 cut(s) 348, 1095
Ecl136II GAGCTC 1 cut(s) 724
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 726
Eco32I GATATC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 724
Eco57I CTGAAG 3 cut(s) 68, 512, 612
Eco88I CYCGRG 1 cut(s) 733
EcoICRI GAGCTC 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 1021
EcoRV GATATC 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 726
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 6 cut(s) 94, 589, 1047, 1130, 1340, 1347
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 1187
FatI CATG 6 cut(s) 90, 585, 1043, 1126, 1336, 1343
FauI CCCGC 1 cut(s) 1051
Fnu4HI GCNGC 4 cut(s) 257, 414, 846, 1056
FokI GGATG 2 cut(s) 28, 290
FriOI GRGCYC 1 cut(s) 726
Fsp4HI GCNGC 4 cut(s) 257, 414, 846, 1056
FspBI CTAG 1 cut(s) 219
GlaI GCGC 1 cut(s) 762
GluI GCNGC 4 cut(s) 257, 414, 846, 1056
GsaI CCCAGC 1 cut(s) 477
HaeII RGCGCY 1 cut(s) 764
HaeIII GGCC 4 cut(s) 316, 584, 680, 1265
HapII CCGG 3 cut(s) 674, 773, 1320
HhaI GCGC 1 cut(s) 763
Hin1II CATG 6 cut(s) 94, 589, 1047, 1130, 1340, 1347
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HindIII AAGCTT 1 cut(s) 186
HinfI GANTC 2 cut(s) 515, 1109
HpaI GTTAAC 1 cut(s) 447
HpaII CCGG 3 cut(s) 674, 773, 1320
HphI GGTGA 1 cut(s) 120
Hpy166II GTNNAC 3 cut(s) 216, 230, 447
Hpy188I TCNGA 2 cut(s) 140, 592
Hpy188III TCNNGA 8 cut(s) 30, 383, 436, 523, 555, 773, 1211, 1337
Hpy8I GTNNAC 3 cut(s) 216, 230, 447
HpyAV CCTTC 2 cut(s) 632, 897
HpyCH4III ACNGT 2 cut(s) 543, 1123
HpyCH4IV ACGT 3 cut(s) 247, 321, 808
HpyCH4V TGCA 5 cut(s) 166, 259, 848, 1047, 1184
HpyF10VI GCNNNNNNNGC 3 cut(s) 677, 854, 1271
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 3 cut(s) 247, 321, 808
Hsp92II CATG 6 cut(s) 94, 589, 1047, 1130, 1340, 1347
HspAI GCGC 1 cut(s) 761
Kpn2I TCCGGA 1 cut(s) 772
KpnI GGTACC 1 cut(s) 1013
KspAI GTTAAC 1 cut(s) 447
Kzo9I GATC 1 cut(s) 607
LmnI GCTCC 4 cut(s) 385, 676, 1245, 1322
Lsp1109I GCAGC 4 cut(s) 243, 400, 832, 1067
LweI GCATC 3 cut(s) 243, 937, 1171
MaeI CTAG 1 cut(s) 219
MaeII ACGT 3 cut(s) 247, 321, 808
MaeIII GTNAC 1 cut(s) 154
MalI GATC 1 cut(s) 609
MboI GATC 1 cut(s) 607
MboII GAAGA 8 cut(s) 365, 505, 605, 608, 611, 637, 1045, 1112
MhlI GDGCHC 1 cut(s) 726
MlsI TGGCCA 3 cut(s) 316, 680, 1265
MluCI AATT 6 cut(s) 431, 694, 984, 1095, 1206, 1217
MluNI TGGCCA 3 cut(s) 316, 680, 1265
MlyI GAGTC 1 cut(s) 1103
Mox20I TGGCCA 3 cut(s) 316, 680, 1265
Mph1103I ATGCAT 1 cut(s) 168
MroI TCCGGA 1 cut(s) 772
MscI TGGCCA 3 cut(s) 316, 680, 1265
MseI TTAA 2 cut(s) 446, 953
MslI CAYNNNNRTG 2 cut(s) 161, 1335
Msp20I TGGCCA 3 cut(s) 316, 680, 1265
MspA1I CMGCKG 1 cut(s) 1058
MspI CCGG 3 cut(s) 674, 773, 1320
MspR9I CCNGG 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 3 cut(s) 677, 854, 1271
NcoI CCATGG 1 cut(s) 90
NdeII GATC 1 cut(s) 607
NlaIII CATG 6 cut(s) 94, 589, 1047, 1130, 1340, 1347
NlaIV GGNNCC 4 cut(s) 294, 381, 1011, 1063
NmuCI GTSAC 1 cut(s) 154
NsiI ATGCAT 1 cut(s) 168
PagI TCATGA 1 cut(s) 1336
PfeI GAWTC 1 cut(s) 515
PkrI GCNGC 4 cut(s) 258, 415, 847, 1057
PleI GAGTC 1 cut(s) 1103
PpsI GAGTC 1 cut(s) 1103
Psp124BI GAGCTC 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 1021
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 4 cut(s) 294, 381, 1011, 1063
PstI CTGCAG 1 cut(s) 261
RsaI GTAC 5 cut(s) 215, 231, 246, 1011, 1125
RsaNI GTAC 5 cut(s) 214, 230, 245, 1010, 1124
RseI CAYNNNNRTG 2 cut(s) 161, 1335
SacI GAGCTC 1 cut(s) 726
SaqAI TTAA 2 cut(s) 446, 953
SatI GCNGC 4 cut(s) 257, 414, 846, 1056
Sau3AI GATC 1 cut(s) 607
SchI GAGTC 1 cut(s) 1103
ScrFI CCNGG 1 cut(s) 1023
SduI GDGCHC 1 cut(s) 726
SfaNI GCATC 3 cut(s) 243, 937, 1171
SfcI CTRYAG 1 cut(s) 257
SmiMI CAYNNNNRTG 2 cut(s) 161, 1335
Sse9I AATT 6 cut(s) 431, 694, 984, 1095, 1206, 1217
SsiI CCGC 1 cut(s) 1058
SspI AATATT 2 cut(s) 1075, 1117
SspMI CTAG 1 cut(s) 219
SstI GAGCTC 1 cut(s) 726
StyD4I CCNGG 1 cut(s) 1021
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 543, 1123
TaiI ACGT 3 cut(s) 250, 324, 811
TaqI TCGA 5 cut(s) 29, 251, 522, 1005, 1154
TasI AATT 6 cut(s) 431, 694, 984, 1095, 1206, 1217
TatI WGTACW 2 cut(s) 213, 1123
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 2 cut(s) 446, 953
Tru9I TTAA 2 cut(s) 446, 953
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 4 cut(s) 256, 413, 845, 1055
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 5 cut(s) 361, 546, 1046, 1230, 1325
XspI CTAG 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.