Rh4AG096400

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000908
Physical Location & Seq
Reverse (-)
20495185 .. 20499986
4802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG096400.1

Sequence Viewer

Length: 930 bp
ATGGACATTCTGGGCGGCTCTCAAGATGGGGACGGATTGGACCTTGAGGGCCAGTTGGTCACGGTCTGTCTGGGAAGCAAAGAGATCGAGGTGGAGGAGGTATTGGTTTCTGGGGTGAGTTTACGTTGTCACTTGGCTGAGAAGAATGTTTCCTATCTTCCAATTTCATCACCACCTGTTCTCTCTATCCATCAGAAACATGATACTACAGGCTCTGTAGAGTTGCCTCTTTCGCTGCAGAAAAAGTTGTTTTCTGGAGAACATACAAAAGAATGTCTTTCGGCAGTTGAAACGATATTTGGATCAGATGCGCCACCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGAGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCAGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTAAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATGCCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTGCGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCCTGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

309

Amino Acids

33.7

Weight (kDa)

4.72

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_10 PF13460 154 - 253 3.1e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 822
AciI CCGC 1 cut(s) 15
AclWI GGATC 2 cut(s) 310, 443
AcsI RAATTY 3 cut(s) 407, 615, 732
AfaI GTAC 2 cut(s) 471, 921
AfiI CCNNNNNNNGG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 7 cut(s) 290, 368, 426, 550, 580, 586, 877
AjnI CCWGG 2 cut(s) 461, 508
AjuI GAANNNNNNNTTGG 4 cut(s) 282, 314, 542, 574
AluBI AGCT 2 cut(s) 349, 415
AluI AGCT 2 cut(s) 349, 415
Alw26I GTCTC 2 cut(s) 499, 684
AlwI GGATC 2 cut(s) 310, 443
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 325
AoxI GGCC 3 cut(s) 49, 497, 635
ApeKI GCWGC 3 cut(s) 235, 346, 743
ApoI RAATTY 3 cut(s) 407, 615, 732
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 2 cut(s) 40, 49
AsuHPI GGTGA 2 cut(s) 127, 162
AvaI CYCGRG 1 cut(s) 325
AvaII GGWCC 1 cut(s) 40
BbvI GCAGC 3 cut(s) 222, 358, 730
BccI CCATC 4 cut(s) 20, 198, 368, 875
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 463, 510
BcoDI GTCTC 2 cut(s) 499, 684
BfmI CTRYAG 5 cut(s) 207, 216, 236, 344, 483
BglII AGATCT 1 cut(s) 670
BisI GCNGC 4 cut(s) 16, 236, 347, 744
BlsI GCNGC 4 cut(s) 17, 237, 348, 745
Bme1390I CCNGG 2 cut(s) 463, 510
Bme18I GGWCC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 325
BmgT120I GGNCC 2 cut(s) 40, 49
BmiI GGNNCC 1 cut(s) 657
BmrFI CCNGG 2 cut(s) 463, 510
BmrI ACTGGG 1 cut(s) 413
BmsI GCATC 1 cut(s) 298
BmuI ACTGGG 1 cut(s) 413
BplI GAGNNNNNCTC 2 cut(s) 657, 689
BpmI CTGGAG 2 cut(s) 276, 684
BpuEI CTTGAG 2 cut(s) 6, 65
BsaAI YACGTR 1 cut(s) 475
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 461, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 847
Bse1I ACTGG 4 cut(s) 52, 419, 772, 923
BseBI CCWGG 2 cut(s) 463, 510
BseDI CCNNGG 2 cut(s) 461, 685
BseGI GGATG 1 cut(s) 886
BseLI CCNNNNNNNGG 1 cut(s) 847
BseMII CTCAG 4 cut(s) 129, 492, 642, 834
BseNI ACTGG 4 cut(s) 52, 419, 772, 923
BseRI GAGGAG 2 cut(s) 110, 668
BseXI GCAGC 3 cut(s) 222, 358, 730
BsgI GTGCAG 1 cut(s) 563
BshFI GGCC 3 cut(s) 51, 499, 637
BsiHKCI CYCGRG 1 cut(s) 325
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 847
BsmAI GTCTC 2 cut(s) 499, 684
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 3 cut(s) 51, 499, 637
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 5 cut(s) 84, 302, 448, 517, 670
BspACI CCGC 1 cut(s) 15
BspANI GGCC 3 cut(s) 51, 499, 637
BspCNI CTCAG 4 cut(s) 130, 493, 643, 835
BspLI GGNNCC 1 cut(s) 657
BspMAI CTGCAG 3 cut(s) 240, 348, 487
BspPI GGATC 2 cut(s) 310, 443
BspTNI GGTCTC 1 cut(s) 499
BsrGI TGTACA 1 cut(s) 469
BsrI ACTGG 4 cut(s) 52, 419, 772, 923
BssECI CCNNGG 2 cut(s) 461, 685
BssMI GATC 5 cut(s) 84, 302, 448, 517, 670
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 2 cut(s) 463, 510
Bst4CI ACNGT 2 cut(s) 64, 820
Bst6I CTCTTC 1 cut(s) 714
BstAUI TGTACA 1 cut(s) 469
BstBAI YACGTR 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 660, 809
BstDEI CTNAG 6 cut(s) 138, 501, 521, 651, 843, 904
BstF5I GGATG 1 cut(s) 886
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 5 cut(s) 87, 305, 451, 520, 673
BstMAI GTCTC 2 cut(s) 499, 684
BstMBI GATC 5 cut(s) 84, 302, 448, 517, 670
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstNI CCWGG 2 cut(s) 463, 510
BstSCI CCNGG 2 cut(s) 461, 508
BstSFI CTRYAG 5 cut(s) 207, 216, 236, 344, 483
BstV1I GCAGC 3 cut(s) 222, 358, 730
BstX2I RGATCY 2 cut(s) 448, 670
BstYI RGATCY 2 cut(s) 448, 670
BsuRI GGCC 3 cut(s) 51, 499, 637
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 1 cut(s) 886
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 1 cut(s) 341
Cac8I GCNNGC 2 cut(s) 660, 809
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 2 cut(s) 40, 49
Csp6I GTAC 2 cut(s) 470, 920
CviAII CATG 1 cut(s) 200
CviQI GTAC 2 cut(s) 470, 920
DdeI CTNAG 6 cut(s) 138, 501, 521, 651, 843, 904
DpnI GATC 5 cut(s) 86, 304, 450, 519, 672
DpnII GATC 5 cut(s) 84, 302, 448, 517, 670
DraI TTTAAA 1 cut(s) 868
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 685
Eco147I AGGCCT 1 cut(s) 637
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 40
Eco88I CYCGRG 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 461, 508
EcoRV GATATC 1 cut(s) 711
EcoT14I CCWWGG 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 1 cut(s) 203
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 1 cut(s) 44
FatI CATG 1 cut(s) 199
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 4 cut(s) 16, 236, 347, 744
FokI GGATG 1 cut(s) 893
Fsp4HI GCNGC 4 cut(s) 16, 236, 347, 744
GlaI GCGC 1 cut(s) 312
GluI GCNGC 4 cut(s) 16, 236, 347, 744
GsuI CTGGAG 2 cut(s) 276, 684
HaeIII GGCC 3 cut(s) 51, 499, 637
HhaI GCGC 1 cut(s) 313
Hin1II CATG 1 cut(s) 203
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HphI GGTGA 2 cut(s) 127, 162
Hpy166II GTNNAC 5 cut(s) 122, 472, 527, 823, 862
Hpy188I TCNGA 3 cut(s) 195, 307, 444
Hpy188III TCNNGA 5 cut(s) 23, 255, 571, 605, 714
Hpy8I GTNNAC 5 cut(s) 122, 472, 527, 823, 862
HpyAV CCTTC 4 cut(s) 395, 580, 841, 871
HpyCH4III ACNGT 2 cut(s) 64, 820
HpyCH4IV ACGT 2 cut(s) 124, 474
HpyCH4V TGCA 6 cut(s) 238, 346, 485, 544, 644, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
HpyF3I CTNAG 6 cut(s) 138, 501, 521, 651, 843, 904
HpySE526I ACGT 2 cut(s) 124, 474
Hsp92II CATG 1 cut(s) 203
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 5 cut(s) 84, 302, 448, 517, 670
LmnI GCTCC 3 cut(s) 420, 655, 665
Lsp1109I GCAGC 3 cut(s) 222, 358, 730
LweI GCATC 1 cut(s) 298
MaeII ACGT 2 cut(s) 124, 474
MaeIII GTNAC 4 cut(s) 58, 128, 339, 490
MalI GATC 5 cut(s) 86, 304, 450, 519, 672
MboI GATC 5 cut(s) 84, 302, 448, 517, 670
MboII GAAGA 3 cut(s) 149, 154, 731
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 2 cut(s) 448, 670
MluCI AATT 9 cut(s) 162, 407, 557, 581, 600, 615, 627, 732, 885
MseI TTAA 5 cut(s) 411, 599, 647, 786, 867
MspR9I CCNGG 2 cut(s) 463, 510
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 2 cut(s) 463, 510
MwoI GCNNNNNNNGC 1 cut(s) 232
NdeII GATC 5 cut(s) 84, 302, 448, 517, 670
NlaIII CATG 1 cut(s) 203
NlaIV GGNNCC 1 cut(s) 657
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 3 cut(s) 58, 128, 339
PceI AGGCCT 1 cut(s) 637
PkrI GCNGC 4 cut(s) 17, 237, 348, 745
Ppu21I YACGTR 1 cut(s) 475
Psp6I CCWGG 2 cut(s) 461, 508
PspGI CCWGG 2 cut(s) 461, 508
PspN4I GGNNCC 1 cut(s) 657
PspPI GGNCC 2 cut(s) 40, 49
PstI CTGCAG 3 cut(s) 240, 348, 487
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 2 cut(s) 448, 670
RsaI GTAC 2 cut(s) 471, 921
RsaNI GTAC 2 cut(s) 470, 920
SaqAI TTAA 5 cut(s) 411, 599, 647, 786, 867
SatI GCNGC 4 cut(s) 16, 236, 347, 744
Sau3AI GATC 5 cut(s) 84, 302, 448, 517, 670
Sau96I GGNCC 2 cut(s) 40, 49
ScrFI CCNGG 2 cut(s) 463, 510
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 5 cut(s) 207, 216, 236, 344, 483
SinI GGWCC 1 cut(s) 40
SmlI CTYRAG 2 cut(s) 21, 44
SmoI CTYRAG 2 cut(s) 21, 44
Sse9I AATT 9 cut(s) 162, 407, 557, 581, 600, 615, 627, 732, 885
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 1 cut(s) 15
SspI AATATT 1 cut(s) 334
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 2 cut(s) 461, 508
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 2 cut(s) 64, 820
TaiI ACGT 2 cut(s) 127, 477
TaqI TCGA 1 cut(s) 87
TasI AATT 9 cut(s) 162, 407, 557, 581, 600, 615, 627, 732, 885
TatI WGTACW 1 cut(s) 469
TauI GCSGC 1 cut(s) 18
Tru1I TTAA 5 cut(s) 411, 599, 647, 786, 867
Tru9I TTAA 5 cut(s) 411, 599, 647, 786, 867
TscAI CASTG 1 cut(s) 348
TseFI GTSAC 3 cut(s) 58, 128, 339
TseI GCWGC 3 cut(s) 235, 346, 743
Tsp45I GTSAC 3 cut(s) 58, 128, 339
TspDTI ATGAA 1 cut(s) 156
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 40
XapI RAATTY 3 cut(s) 407, 615, 732
XmiI GTMKAC 1 cut(s) 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.