pycom05g27430

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000908
Physical Location & Seq
Forward (+)
28410833 .. 28415885
5053 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27430.11

Sequence Viewer

Length: 1995 bp
ATGGAGAAGGAGCCACGTCCATGTTCCAACAAAGAAAGTGACAGTTATTGTGGCCCTTTACTTATTCAGCCCTTCTATATGTGGTGTGTATTTACTCCCACAAATATCAATCATCACTACACAATAAAAAAGCAACAAGATAAAAACCCAGAAGAGTCTGGAATTCAGTTTCCAACTCTCGCTGGAAAATTGGAAATGGAATTAGGTGCCATTAATCTGTCGTCCCCTTTGTCTCCATCAGCCCTCAGAATTCAGGATTTTTCATCACCTGTTGGTAGGAATCATTTTAAAAAATCTTTACATCCTCAGTTGCTGTGCTCTCCACTTCCTAAACCCTTTCTTCAACTTAATGACAGACCACACAGCTTTCTCTACCAACAAAACACTGATAATCTCTCTACGAGAACGTATAGAAGATACATATCAGCAGAAGCTGGAAAGCAAAGTTGGGACTTGACTAGATTCTTGAAAACAATATATTTCTTCAACGAACCGCCCTCTCCTGCTAAGTTTATTGAGGCTTTAATTGAAAAACTATCCGGCCCATCACCTAGTAAACCAGAAAGGAAAATGGAAACTTCTGGCATTGTCCTGGTGGCTGGAGCAACCGGTGGTGTAGGTCGAAGGGCAGTCAACATCTTAAGGAAGAAAGGGTTTCCAGTTCGAGTTAGAAATGTAGAAAAAGCACGGCAGATGTTAGGCCCAGATATCGACTTGGTTGTTGGAGACATTACTAAGGAGAGTACGCTAGTTCCTGAGTACTTCAAAGGTGTGAGGAGAGTGATTAATGCGGTTTCTGTCATTGTTGGTCCAAAGGAAGGTGACACTCCTGATAGGCAAAAGTATAGTCAGGGAATCAAGTTCTTTGAACCAGAGATAAAGGGGGATTCACCCGAAAAGGTGGAGTACATTGGGATGAAAAATTTGATCAATGCTGTAAAAGGAAGCGTCGGACTTCAAAATGGGAAAATACTTTTTGGATTCGAAGGCAATAGTTTTAAGGAAATGGCTTGGGGTGCTTTAGATGATGTTGTGATGGGTGGAGTGAGTGAAAGTACATTTCAGATTGACCGAAACGGTGGTGAAAATGGTGGACCAACTGGACTTTTTAAAGGGGTTGTTTCCACTGCAAACAATGGTGGTTTTACTAGTATCAGAACAAAGAATCTACCAACAGCTGAAGATCTCTCTGCCTATGATGGCTTGGAGCTACGCCTTAAAGGTGATGGTCGACGCTATAAGCTAATTGTTCGCACAAGCCGTGATTGGGATACTGTTGGTTACACAGCAGGTTTTGACACTGTTGGAAACCAGTGGCAGACAGTACGCATACCATTTTCTTCTTTGAAACCAATATTTCGAGCACGAACTGTATCAGATGCGCCACCGTTTGACCCTAGCAACGTTGTGTCGTTGCAGCTCATGTTTAGCAAGTTTGAATACGATGGGAAACTGAATCCTACTTTTGTGGAAGGCCCATTTCAGCTCCCATTATCAAGCATACGGGCATATCTAAAAGAACCTATAACTCCCAGGTTTGTACATGTAGGTTCTGCAGGAGTCACCCGACCTGAGAGGCCTGGACTTGACCTAAGTAAACAACCTCCCGCTGTTCGCTTGAACAAGGAATTGGATTTTATCCTTACATACAAGTTGAAGGGGGAGGATTTAATACGGGAAAGTGGAATACCATACACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCCGGAGCAGATCTCATTTTTGATCAAGGAGACAATATAACGGGTAAGATTTCAAGAGAGGAGGTTGCTCAAATTTGCATTGCTGCTTTAGAAAGCCCGTATGCAACTGGCAAGACATTTGAGGTTAAGAGTGTCATACCATTTAGCCAGCCGTTTACAGTGGATCCCGAAAACCCACCTCCCGAGAAGGACTACGATAGCTCTGCTATCAACATTAACTCTGTGTATATAGTCATAGCAGATGAAAAGCAGAGGATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

665

Amino Acids

73.71

Weight (kDa)

8.69

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1280
AccB1I GGYRCC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 1231
AciI CCGC 3 cut(s) 494, 791, 1608
AclI AACGTT 1 cut(s) 1404
AclWI GGATC 2 cut(s) 1889, 1902
AcsI RAATTY 4 cut(s) 162, 249, 922, 1803
AcuI CTGAAG 1 cut(s) 1200
AfaI GTAC 6 cut(s) 745, 761, 908, 1057, 1326, 1542
AfiI CCNNNNNNNGG 2 cut(s) 818, 1267
AflII CTTAAG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 1543
AgeI ACCGGT 1 cut(s) 608
AhlI ACTAGT 1 cut(s) 1148
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 3 cut(s) 591, 1532, 1579
AjuI GAANNNNNNNTTGG 4 cut(s) 430, 462, 1613, 1645
AleI CACNNNNGTG 1 cut(s) 1700
AluBI AGCT 8 cut(s) 366, 434, 1178, 1210, 1243, 1420, 1486, 1932
AluI AGCT 8 cut(s) 366, 434, 1178, 1210, 1243, 1420, 1486, 1932
Alw21I GWGCWC 2 cut(s) 320, 1366
Alw26I GTCTC 3 cut(s) 237, 720, 1755
AlwI GGATC 2 cut(s) 1889, 1902
AlwNI CAGNNNCTG 2 cut(s) 313, 434
Ama87I CYCGRG 1 cut(s) 1913
AoxI GGCC 6 cut(s) 52, 541, 700, 1474, 1577, 1706
ApeKI GCWGC 2 cut(s) 1417, 1814
ApoI RAATTY 4 cut(s) 162, 249, 922, 1803
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
AseI ATTAAT 2 cut(s) 213, 786
AsiGI ACCGGT 1 cut(s) 608
AspLEI GCGC 1 cut(s) 1384
AspS9I GGNCC 6 cut(s) 53, 542, 701, 809, 1094, 1475
AsuHPI GGTGA 7 cut(s) 258, 540, 833, 882, 1094, 1235, 1555
AsuII TTCGAA 1 cut(s) 984
AvaI CYCGRG 1 cut(s) 1913
AvaII GGWCC 2 cut(s) 809, 1094
BamHI GGATCC 1 cut(s) 1894
BanI GGYRCC 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 320, 1366
BbvI GCAGC 2 cut(s) 1429, 1801
BccI CCATC 6 cut(s) 244, 553, 1030, 1193, 1220, 1439
BceAI ACGGC 3 cut(s) 704, 1245, 1867
BcgI CGANNNNNNTGC 2 cut(s) 1916, 1950
BciT130I CCWGG 3 cut(s) 593, 1534, 1581
BciVI GTATCC 1 cut(s) 1264
BclI TGATCA 2 cut(s) 927, 1753
BcoDI GTCTC 3 cut(s) 237, 720, 1755
BcuI ACTAGT 1 cut(s) 1148
BfaI CTAG 5 cut(s) 459, 552, 749, 1149, 1398
BfmI CTRYAG 1 cut(s) 1554
BfrI CTTAAG 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 1280
BfuI GTATCC 1 cut(s) 1264
BglII AGATCT 2 cut(s) 1183, 1741
BisI GCNGC 2 cut(s) 1418, 1815
BlsI GCNGC 2 cut(s) 1419, 1816
BmcAI AGTACT 1 cut(s) 761
Bme1390I CCNGG 3 cut(s) 593, 1534, 1581
Bme18I GGWCC 2 cut(s) 809, 1094
BmeT110I CYCGRG 1 cut(s) 1913
BmgBI CACGTC 1 cut(s) 17
BmgT120I GGNCC 6 cut(s) 53, 542, 701, 809, 1094, 1475
BmiI GGNNCC 4 cut(s) 12, 208, 1728, 1896
BmrFI CCNGG 3 cut(s) 593, 1534, 1581
BmsI GCATC 1 cut(s) 1369
BplI GAGNNNNNCTC 2 cut(s) 1728, 1760
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 984
BsaBI GATNNNNATC 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 1532
BsaWI WCCGGW 1 cut(s) 608
Bsc4I CCNNNNNNNGG 2 cut(s) 818, 1267
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 4 cut(s) 659, 1105, 1312, 1843
Bse3DI GCAATG 1 cut(s) 1809
Bse8I GATNNNNATC 1 cut(s) 421
BseBI CCWGG 3 cut(s) 593, 1534, 1581
BseDI CCNNGG 1 cut(s) 1532
BseGI GGATG 2 cut(s) 301, 921
BseJI GATNNNNATC 1 cut(s) 421
BseLI CCNNNNNNNGG 2 cut(s) 818, 1267
BseMI GCAATG 1 cut(s) 1809
BseMII CTCAG 5 cut(s) 259, 320, 747, 1563, 1713
BseNI ACTGG 4 cut(s) 659, 1105, 1312, 1843
BseRI GAGGAG 3 cut(s) 790, 1739, 1805
BseXI GCAGC 2 cut(s) 1429, 1801
BshFI GGCC 6 cut(s) 54, 543, 702, 1476, 1579, 1708
BshNI GGYRCC 1 cut(s) 206
BshTI ACCGGT 1 cut(s) 608
BsiHKAI GWGCWC 2 cut(s) 320, 1366
BsiHKCI CYCGRG 1 cut(s) 1913
BsiSI CCGG 3 cut(s) 540, 609, 1734
BslFI GGGAC 2 cut(s) 208, 464
BslI CCNNNNNNNGG 2 cut(s) 818, 1267
BsmAI GTCTC 3 cut(s) 237, 720, 1755
BsmFI GGGAC 2 cut(s) 208, 464
BsnI GGCC 6 cut(s) 54, 543, 702, 1476, 1579, 1708
BsoBI CYCGRG 1 cut(s) 1913
Bsp119I TTCGAA 1 cut(s) 984
Bsp1286I GDGCHC 2 cut(s) 320, 1366
Bsp1407I TGTACA 1 cut(s) 1540
Bsp143I GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
BspACI CCGC 3 cut(s) 494, 791, 1608
BspANI GGCC 6 cut(s) 54, 543, 702, 1476, 1579, 1708
BspCNI CTCAG 5 cut(s) 258, 319, 748, 1564, 1714
BspLI GGNNCC 4 cut(s) 12, 208, 1728, 1896
BspMAI CTGCAG 1 cut(s) 1558
BspMI ACCTGC 1 cut(s) 1280
BspPI GGATC 2 cut(s) 1889, 1902
BspT104I TTCGAA 1 cut(s) 984
BspT107I GGYRCC 1 cut(s) 206
BspTI CTTAAG 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 1809
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 1540
BsrI ACTGG 4 cut(s) 659, 1105, 1312, 1843
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 1532
BssMI GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
Bst2UI CCWGG 3 cut(s) 593, 1534, 1581
Bst4CI ACNGT 8 cut(s) 44, 1079, 1276, 1303, 1324, 1372, 1389, 1891
Bst6I CTCTTC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 640
BstAUI TGTACA 1 cut(s) 1540
BstBI TTCGAA 1 cut(s) 984
BstC8I GCNNGC 2 cut(s) 1731, 1880
BstDEI CTNAG 8 cut(s) 245, 306, 507, 735, 756, 1572, 1592, 1722
BstF5I GGATG 2 cut(s) 301, 921
BstHHI GCGC 1 cut(s) 1384
BstKTI GATC 5 cut(s) 930, 1186, 1744, 1756, 1897
BstMAI GTCTC 3 cut(s) 237, 720, 1755
BstMBI GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
BstMWI GCNNNNNNNGC 1 cut(s) 1016
BstNI CCWGG 3 cut(s) 593, 1534, 1581
BstNSI RCATGY 1 cut(s) 1547
BstSCI CCNGG 3 cut(s) 591, 1532, 1579
BstSFI CTRYAG 1 cut(s) 1554
BstV1I GCAGC 2 cut(s) 1429, 1801
BstX2I RGATCY 3 cut(s) 1183, 1741, 1894
BstYI RGATCY 3 cut(s) 1183, 1741, 1894
BsuI GTATCC 1 cut(s) 1264
BsuRI GGCC 6 cut(s) 54, 543, 702, 1476, 1579, 1708
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 2 cut(s) 301, 921
BtsI GCAGTG 1 cut(s) 1125
BtsIMutI CAGTG 5 cut(s) 384, 1125, 1299, 1319, 1896
BveI ACCTGC 1 cut(s) 1280
Cac8I GCNNGC 2 cut(s) 1731, 1880
CaiI CAGNNNCTG 2 cut(s) 313, 434
CfoI GCGC 1 cut(s) 1384
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 6 cut(s) 53, 542, 701, 809, 1094, 1475
CseI GACGC 2 cut(s) 937, 1242
Csp6I GTAC 6 cut(s) 744, 760, 907, 1056, 1325, 1541
CspAI ACCGGT 1 cut(s) 608
CviAII CATG 3 cut(s) 21, 1423, 1544
CviQI GTAC 6 cut(s) 744, 760, 907, 1056, 1325, 1541
DdeI CTNAG 8 cut(s) 245, 306, 507, 735, 756, 1572, 1592, 1722
DpnI GATC 5 cut(s) 929, 1185, 1743, 1755, 1896
DpnII GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
DraI TTTAAA 2 cut(s) 289, 1111
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco147I AGGCCT 2 cut(s) 1579, 1708
Eco32I GATATC 1 cut(s) 709
Eco47I GGWCC 2 cut(s) 809, 1094
Eco57I CTGAAG 1 cut(s) 1200
Eco88I CYCGRG 1 cut(s) 1913
EcoRI GAATTC 2 cut(s) 162, 249
EcoRII CCWGG 3 cut(s) 591, 1532, 1579
EcoRV GATATC 1 cut(s) 709
FaeI CATG 3 cut(s) 24, 1426, 1547
FaqI GGGAC 2 cut(s) 208, 464
FatI CATG 3 cut(s) 20, 1422, 1543
FauI CCCGC 1 cut(s) 1615
FbaI TGATCA 2 cut(s) 927, 1753
FblI GTMKAC 1 cut(s) 1231
Fnu4HI GCNGC 2 cut(s) 1418, 1815
FokI GGATG 2 cut(s) 288, 928
Fsp4HI GCNGC 2 cut(s) 1418, 1815
FspBI CTAG 5 cut(s) 459, 552, 749, 1149, 1398
GlaI GCGC 1 cut(s) 1383
GluI GCNGC 2 cut(s) 1418, 1815
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 6 cut(s) 54, 543, 702, 1476, 1579, 1708
HapII CCGG 3 cut(s) 540, 609, 1734
HgaI GACGC 2 cut(s) 937, 1242
HhaI GCGC 1 cut(s) 1384
Hin1II CATG 3 cut(s) 24, 1426, 1547
Hin6I GCGC 1 cut(s) 1382
HinP1I GCGC 1 cut(s) 1382
HincII GTYRAC 2 cut(s) 634, 1232
HindII GTYRAC 2 cut(s) 634, 1232
HpaII CCGG 3 cut(s) 540, 609, 1734
HphI GGTGA 7 cut(s) 258, 540, 833, 882, 1094, 1235, 1555
Hpy166II GTNNAC 6 cut(s) 557, 634, 1094, 1232, 1598, 1887
Hpy188I TCNGA 5 cut(s) 248, 953, 1065, 1157, 1378
Hpy188III TCNNGA 8 cut(s) 159, 254, 466, 755, 830, 1785, 1898, 1913
Hpy8I GTNNAC 6 cut(s) 557, 634, 1094, 1232, 1598, 1887
Hpy99I CGWCG 2 cut(s) 953, 1236
HpyAV CCTTC 7 cut(s) 82, 618, 812, 980, 1466, 1651, 1912
HpyCH4III ACNGT 8 cut(s) 44, 1079, 1276, 1303, 1324, 1372, 1389, 1891
HpyCH4IV ACGT 3 cut(s) 16, 407, 1404
HpyCH4V TGCA 6 cut(s) 1130, 1417, 1556, 1715, 1809, 1835
HpyF10VI GCNNNNNNNGC 1 cut(s) 1016
HpyF3I CTNAG 8 cut(s) 245, 306, 507, 735, 756, 1572, 1592, 1722
HpySE526I ACGT 3 cut(s) 16, 407, 1404
Hsp92II CATG 3 cut(s) 24, 1426, 1547
HspAI GCGC 1 cut(s) 1382
Ksp22I TGATCA 2 cut(s) 927, 1753
Kzo9I GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
LmnI GCTCC 6 cut(s) 10, 602, 1207, 1491, 1726, 1736
Lsp1109I GCAGC 2 cut(s) 1429, 1801
LweI GCATC 1 cut(s) 1369
MaeI CTAG 5 cut(s) 459, 552, 749, 1149, 1398
MaeII ACGT 3 cut(s) 16, 407, 1404
MaeIII GTNAC 4 cut(s) 38, 821, 1280, 1561
MalI GATC 5 cut(s) 929, 1185, 1743, 1755, 1896
MboI GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
MboII GAAGA 7 cut(s) 164, 332, 426, 475, 658, 1193, 1332
MfeI CAATTG 1 cut(s) 1698
MflI RGATCY 3 cut(s) 1183, 1741, 1894
MhlI GDGCHC 2 cut(s) 320, 1366
MlyI GAGTC 2 cut(s) 164, 1569
MmeI TCCRAC 5 cut(s) 51, 197, 703, 931, 1285
MslI CAYNNNNRTG 3 cut(s) 19, 914, 1700
MspA1I CMGCKG 2 cut(s) 1178, 1610
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 3 cut(s) 540, 609, 1734
MspR9I CCNGG 3 cut(s) 593, 1534, 1581
MunI CAATTG 1 cut(s) 1698
MvaI CCWGG 3 cut(s) 593, 1534, 1581
MwoI GCNNNNNNNGC 1 cut(s) 1016
NdeII GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
NlaIII CATG 3 cut(s) 24, 1426, 1547
NlaIV GGNNCC 4 cut(s) 12, 208, 1728, 1896
NmuCI GTSAC 3 cut(s) 38, 821, 1561
NspI RCATGY 1 cut(s) 1547
NspV TTCGAA 1 cut(s) 984
OliI CACNNNNGTG 1 cut(s) 1700
PceI AGGCCT 2 cut(s) 1579, 1708
PciI ACATGT 1 cut(s) 1543
PcsI WCGNNNNNNNCGW 1 cut(s) 1258
PfeI GAWTC 8 cut(s) 280, 462, 855, 887, 981, 1165, 1456, 1987
PinAI ACCGGT 1 cut(s) 608
PkrI GCNGC 2 cut(s) 1419, 1816
PleI GAGTC 2 cut(s) 163, 1568
PpsI GAGTC 2 cut(s) 163, 1568
PscI ACATGT 1 cut(s) 1543
PshBI ATTAAT 2 cut(s) 213, 786
Psp1406I AACGTT 1 cut(s) 1404
Psp6I CCWGG 3 cut(s) 591, 1532, 1579
PspGI CCWGG 3 cut(s) 591, 1532, 1579
PspN4I GGNNCC 4 cut(s) 12, 208, 1728, 1896
PspPI GGNCC 6 cut(s) 53, 542, 701, 809, 1094, 1475
PstI CTGCAG 1 cut(s) 1558
PstNI CAGNNNCTG 2 cut(s) 313, 434
PsuI RGATCY 3 cut(s) 1183, 1741, 1894
PvuII CAGCTG 1 cut(s) 1178
RsaI GTAC 6 cut(s) 745, 761, 908, 1057, 1326, 1542
RsaNI GTAC 6 cut(s) 744, 760, 907, 1056, 1325, 1541
RseI CAYNNNNRTG 3 cut(s) 19, 914, 1700
SalI GTCGAC 1 cut(s) 1230
SatI GCNGC 2 cut(s) 1418, 1815
Sau3AI GATC 5 cut(s) 927, 1183, 1741, 1753, 1894
Sau96I GGNCC 6 cut(s) 53, 542, 701, 809, 1094, 1475
ScaI AGTACT 1 cut(s) 761
SchI GAGTC 2 cut(s) 164, 1569
ScrFI CCNGG 3 cut(s) 593, 1534, 1581
SduI GDGCHC 2 cut(s) 320, 1366
SfaNI GCATC 1 cut(s) 1369
SfcI CTRYAG 1 cut(s) 1554
SfuI TTCGAA 1 cut(s) 984
SinI GGWCC 2 cut(s) 809, 1094
SmiMI CAYNNNNRTG 3 cut(s) 19, 914, 1700
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SpeI ACTAGT 1 cut(s) 1148
SseBI AGGCCT 2 cut(s) 1579, 1708
SsiI CCGC 3 cut(s) 494, 791, 1608
SspI AATATT 1 cut(s) 1356
SspMI CTAG 5 cut(s) 459, 552, 749, 1149, 1398
StuI AGGCCT 2 cut(s) 1579, 1708
StyD4I CCNGG 3 cut(s) 591, 1532, 1579
TaaI ACNGT 8 cut(s) 44, 1079, 1276, 1303, 1324, 1372, 1389, 1891
TaiI ACGT 3 cut(s) 19, 410, 1407
TaqI TCGA 6 cut(s) 622, 664, 711, 984, 1231, 1360
TaqII GACCGA 1 cut(s) 1086
TatI WGTACW 4 cut(s) 759, 906, 1055, 1540
TfiI GAWTC 8 cut(s) 280, 462, 855, 887, 981, 1165, 1456, 1987
TscAI CASTG 5 cut(s) 391, 1132, 1306, 1319, 1896
TseFI GTSAC 3 cut(s) 38, 821, 1561
TseI GCWGC 2 cut(s) 1417, 1814
Tsp45I GTSAC 3 cut(s) 38, 821, 1561
TspDTI ATGAA 3 cut(s) 252, 932, 1989
TspRI CASTG 5 cut(s) 391, 1132, 1306, 1319, 1896
Vha464I CTTAAG 1 cut(s) 640
VpaK11BI GGWCC 2 cut(s) 809, 1094
VspI ATTAAT 2 cut(s) 213, 786
XapI RAATTY 4 cut(s) 162, 249, 922, 1803
XceI RCATGY 1 cut(s) 1547
XmiI GTMKAC 1 cut(s) 1231
XspI CTAG 5 cut(s) 459, 552, 749, 1149, 1398
ZrmI AGTACT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.