Rw6G021670

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000908
Physical Location & Seq
Forward (+)
43125886 .. 43129045
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G021670.1

Sequence Viewer

Length: 1197 bp
ATGGACATTCTGGGCGGCTCTGAAGATGGGGACGGATTGGACCTTGAGGGCCAGTTGGTCACGGTCTGTCTGGGAAGCAAAGAGATCGAGGTGGAGGAGGTATTGGTTTCTGGGGTGGTAGGTGGCATCGAGGAGGCACCAGGAGAGGAGGTGGGCCAAGGAAGGAGGGGTCGGATCGGAGGAGTTGTTGTCGTGGTAGACGACGCGGTGGATCTGTTGGGTCGGGAGAGAAACTGGTCGGGTGGGATATGGGGGTTGGGGGACGAAGGTGGGGGAGGAGGAAGAAGAGGGTGGGGAGAGTGTGGCGTAGGAGTTGTTTCCACTGCAAATAACGGTGGGTTTACAAGCATCAGAACAAAGAATCTATCAACACCTGAGGATCTCTCTGCATATGATGGTTTAGAGCTACGCCTTAAAGGTGATGGTCGTCGTTATAAACTAATTGTTCGGACAAGTGGTGATTGGGATACTGTTGGTTACACAGCAGGCTTTGACACTGTAAAAGACCAGTGGCAGACAGTACGGCTTCCATTTTCTTCTTTGAGGCCTATTTTTCGTGCAAAAACTGTATCAGATGCGCCACCATTTGACCCGAGCAATATTGTGTCACTGCAGCTTATGTTCAGCAAGTTTGAATATGATGGAAAACTCAATCCTACTTTTGTGGAAGGGGAATTTAAGCTCCCAGTTTCAAGCATAAGGGCATATCTGAAAGATCCTATAACTCCCAGGTTTGTACACGTAGGCTCTGCAGGAGTTACTCGGCCTGAGAGACCTGGACTTGATCTAAGTAAACAACCTCCTGCTGTGCGGTTGAACAAGGAATTGGATTTTATCCTGACTTTCAAATTGAAGGGGGAGGATTTAATTCGGGAAAGTGGAATTCCATATACAATTGTGAGGCCTTGTGCATTAACTGAGGAGCCTGCTGGAGCAGATCTCATTTTTGACCAAGGAGACAATATAACGGGTAAGATATCAAGAGAAGAGGTTGCTCAAATTTGTGTTGCTGCATTAGAAAGCCCATACGCTACTGGCAAGACATTTGAGGTTAAAAGTGTTATGCCATTTAGCGAGCCATTTACAGTAGACCCTGAAAATCCACCCCCTGAGAAGGACTGCGATGTTTACTTTAAAACTTTGAAGGATGGAATTACAGGAAAAGAAGTCTTAGAACAAAATCCTGTACCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CIA30 NAD_binding_10

Protein Analysis

398

Amino Acids

43.05

Weight (kDa)

4.71

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CIA30 PF08547 104 - 229 1.1e-32 Complex I intermediate-associated protein 30 (CIA30)
NAD_binding_10 PF13460 243 - 342 5.4e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 2 cut(s) 198, 1089
AccII CGCG 1 cut(s) 206
AciI CCGC 3 cut(s) 15, 206, 811
AclWI GGATC 4 cut(s) 182, 219, 387, 710
AcsI RAATTY 3 cut(s) 674, 882, 999
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 3 cut(s) 522, 738, 1188
AfiI CCNNNNNNNGG 1 cut(s) 1114
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 6 cut(s) 635, 693, 817, 847, 853, 1144
AjnI CCWGG 3 cut(s) 139, 728, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AluBI AGCT 3 cut(s) 406, 616, 682
AluI AGCT 3 cut(s) 406, 616, 682
Alw26I GTCTC 2 cut(s) 766, 951
AlwI GGATC 4 cut(s) 182, 219, 387, 710
Ama87I CYCGRG 1 cut(s) 592
AoxI GGCC 5 cut(s) 49, 154, 545, 764, 902
ApeKI GCWGC 2 cut(s) 613, 1010
ApoI RAATTY 3 cut(s) 674, 882, 999
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 3 cut(s) 40, 49, 154
AsuHPI GGTGA 2 cut(s) 431, 470
AvaI CYCGRG 1 cut(s) 592
AvaII GGWCC 1 cut(s) 40
AxyI CCTNAGG 1 cut(s) 375
BanI GGYRCC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 625, 997
BccI CCATC 5 cut(s) 20, 389, 416, 635, 1142
BceAI ACGGC 1 cut(s) 539
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 3 cut(s) 141, 730, 777
BciVI GTATCC 1 cut(s) 460
BcoDI GTCTC 2 cut(s) 766, 951
BfmI CTRYAG 2 cut(s) 611, 750
BfuI GTATCC 1 cut(s) 460
BglII AGATCT 1 cut(s) 937
BisI GCNGC 3 cut(s) 16, 614, 1011
BlsI GCNGC 3 cut(s) 17, 615, 1012
Bme1390I CCNGG 3 cut(s) 141, 730, 777
Bme18I GGWCC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 40, 49, 154
BmiI GGNNCC 2 cut(s) 138, 924
BmrFI CCNGG 3 cut(s) 141, 730, 777
BmrI ACTGGG 1 cut(s) 680
BmsI GCATC 3 cut(s) 135, 357, 565
BmuI ACTGGG 1 cut(s) 680
BplI GAGNNNNNCTC 4 cut(s) 368, 400, 924, 956
BpmI CTGGAG 1 cut(s) 951
BpuEI CTTGAG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 742
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 3 cut(s) 157, 728, 952
BsaXI ACNNNNNCTCC 6 cut(s) 135, 165, 171, 174, 201, 204
Bsc4I CCNNNNNNNGG 1 cut(s) 1114
Bse1I ACTGG 6 cut(s) 52, 239, 508, 686, 1039, 1190
Bse21I CCTNAGG 1 cut(s) 375
BseBI CCWGG 3 cut(s) 141, 730, 777
BseDI CCNNGG 3 cut(s) 157, 728, 952
BseGI GGATG 1 cut(s) 1153
BseLI CCNNNNNNNGG 1 cut(s) 1114
BseMII CTCAG 4 cut(s) 366, 759, 909, 1101
BseNI ACTGG 6 cut(s) 52, 239, 508, 686, 1039, 1190
BseRI GAGGAG 6 cut(s) 110, 146, 161, 195, 291, 935
BseXI GCAGC 2 cut(s) 625, 997
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 5 cut(s) 51, 156, 547, 766, 904
BshNI GGYRCC 1 cut(s) 136
BsiHKCI CYCGRG 1 cut(s) 592
BslFI GGGAC 2 cut(s) 44, 275
BslI CCNNNNNNNGG 1 cut(s) 1114
BsmAI GTCTC 2 cut(s) 766, 951
BsmFI GGGAC 2 cut(s) 44, 275
BsnI GGCC 5 cut(s) 51, 156, 547, 766, 904
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 592
Bsp1407I TGTACA 1 cut(s) 736
Bsp143I GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
BspACI CCGC 3 cut(s) 15, 206, 811
BspANI GGCC 5 cut(s) 51, 156, 547, 766, 904
BspCNI CTCAG 4 cut(s) 367, 760, 910, 1102
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 2 cut(s) 138, 924
BspMAI CTGCAG 2 cut(s) 615, 754
BspPI GGATC 4 cut(s) 182, 219, 387, 710
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 766
BsrGI TGTACA 1 cut(s) 736
BsrI ACTGG 6 cut(s) 52, 239, 508, 686, 1039, 1190
BssECI CCNNGG 3 cut(s) 157, 728, 952
BssMI GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
BssT1I CCWWGG 2 cut(s) 157, 952
Bst2UI CCWGG 3 cut(s) 141, 730, 777
Bst4CI ACNGT 7 cut(s) 64, 335, 472, 499, 520, 568, 1087
Bst6I CTCTTC 2 cut(s) 280, 981
BstAUI TGTACA 1 cut(s) 736
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 3 cut(s) 487, 927, 1076
BstDEI CTNAG 6 cut(s) 375, 768, 788, 918, 1110, 1171
BstF5I GGATG 1 cut(s) 1153
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 7 cut(s) 87, 177, 214, 382, 718, 787, 940
BstMAI GTCTC 2 cut(s) 766, 951
BstMBI GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
BstNI CCWGG 3 cut(s) 141, 730, 777
BstSCI CCNGG 3 cut(s) 139, 728, 775
BstSFI CTRYAG 2 cut(s) 611, 750
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 625, 997
BstX2I RGATCY 4 cut(s) 211, 379, 715, 937
BstYI RGATCY 4 cut(s) 211, 379, 715, 937
Bsu36I CCTNAGG 1 cut(s) 375
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 5 cut(s) 51, 156, 547, 766, 904
BtgZI GCGATG 1 cut(s) 1137
BtsCI GGATG 1 cut(s) 1153
BtsI GCAGTG 2 cut(s) 321, 608
BtsIMutI CAGTG 4 cut(s) 321, 495, 515, 608
Cac8I GCNNGC 3 cut(s) 487, 927, 1076
CfoI GCGC 1 cut(s) 580
Cfr13I GGNCC 3 cut(s) 40, 49, 154
CseI GACGC 1 cut(s) 212
Csp6I GTAC 3 cut(s) 521, 737, 1187
CviQI GTAC 3 cut(s) 521, 737, 1187
DdeI CTNAG 6 cut(s) 375, 768, 788, 918, 1110, 1171
DpnI GATC 7 cut(s) 86, 176, 213, 381, 717, 786, 939
DpnII GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
DraI TTTAAA 1 cut(s) 1135
Eam1104I CTCTTC 2 cut(s) 280, 981
EarI CTCTTC 2 cut(s) 280, 981
Eco130I CCWWGG 2 cut(s) 157, 952
Eco147I AGGCCT 2 cut(s) 547, 904
Eco31I GGTCTC 1 cut(s) 766
Eco32I GATATC 1 cut(s) 978
Eco47I GGWCC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 42
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 882
EcoRII CCWGG 3 cut(s) 139, 728, 775
EcoRV GATATC 1 cut(s) 978
EcoT14I CCWWGG 2 cut(s) 157, 952
ErhI CCWWGG 2 cut(s) 157, 952
FaqI GGGAC 2 cut(s) 44, 275
FauNDI CATATG 1 cut(s) 391
FblI GTMKAC 2 cut(s) 198, 1089
Fnu4HI GCNGC 3 cut(s) 16, 614, 1011
FokI GGATG 1 cut(s) 1160
Fsp4HI GCNGC 3 cut(s) 16, 614, 1011
GlaI GCGC 1 cut(s) 579
GluI GCNGC 3 cut(s) 16, 614, 1011
GsuI CTGGAG 1 cut(s) 951
HaeIII GGCC 5 cut(s) 51, 156, 547, 766, 904
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 580
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HinfI GANTC 1 cut(s) 361
HphI GGTGA 2 cut(s) 431, 470
Hpy166II GTNNAC 6 cut(s) 199, 342, 739, 794, 1090, 1129
Hpy188I TCNGA 7 cut(s) 22, 174, 179, 353, 450, 574, 711
Hpy188III TCNNGA 4 cut(s) 224, 838, 872, 981
Hpy8I GTNNAC 6 cut(s) 199, 342, 739, 794, 1090, 1129
Hpy99I CGWCG 2 cut(s) 206, 432
HpyAV CCTTC 6 cut(s) 156, 260, 662, 847, 1108, 1138
HpyCH4III ACNGT 7 cut(s) 64, 335, 472, 499, 520, 568, 1087
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 7 cut(s) 326, 389, 560, 613, 752, 911, 1013
HpyF3I CTNAG 6 cut(s) 375, 768, 788, 918, 1110, 1171
HpySE526I ACGT 1 cut(s) 741
HspAI GCGC 1 cut(s) 578
Kzo9I GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
LmnI GCTCC 3 cut(s) 687, 922, 932
Lsp1109I GCAGC 2 cut(s) 625, 997
LweI GCATC 3 cut(s) 135, 357, 565
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 4 cut(s) 58, 476, 606, 757
MalI GATC 7 cut(s) 86, 176, 213, 381, 717, 786, 939
MboI GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
MboII GAAGA 5 cut(s) 35, 294, 297, 528, 998
MfeI CAATTG 1 cut(s) 894
MflI RGATCY 4 cut(s) 211, 379, 715, 937
MluCI AATT 9 cut(s) 441, 674, 824, 848, 867, 882, 894, 999, 1152
MmeI TCCRAC 1 cut(s) 152
MseI TTAA 6 cut(s) 414, 678, 866, 914, 1053, 1134
MspR9I CCNGG 3 cut(s) 141, 730, 777
MunI CAATTG 1 cut(s) 894
MvaI CCWGG 3 cut(s) 141, 730, 777
MvnI CGCG 1 cut(s) 206
NdeI CATATG 1 cut(s) 391
NdeII GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
NlaIV GGNNCC 2 cut(s) 138, 924
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 2 cut(s) 58, 606
PceI AGGCCT 2 cut(s) 547, 904
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 3 cut(s) 17, 615, 1012
Ppu21I YACGTR 1 cut(s) 742
PsiI TTATAA 1 cut(s) 435
Psp6I CCWGG 3 cut(s) 139, 728, 775
PspGI CCWGG 3 cut(s) 139, 728, 775
PspN4I GGNNCC 2 cut(s) 138, 924
PspPI GGNCC 3 cut(s) 40, 49, 154
PstI CTGCAG 2 cut(s) 615, 754
PsuI RGATCY 4 cut(s) 211, 379, 715, 937
RsaI GTAC 3 cut(s) 522, 738, 1188
RsaNI GTAC 3 cut(s) 521, 737, 1187
SaqAI TTAA 6 cut(s) 414, 678, 866, 914, 1053, 1134
SatI GCNGC 3 cut(s) 16, 614, 1011
Sau3AI GATC 7 cut(s) 84, 174, 211, 379, 715, 784, 937
Sau96I GGNCC 3 cut(s) 40, 49, 154
ScrFI CCNGG 3 cut(s) 141, 730, 777
SfaNI GCATC 3 cut(s) 135, 357, 565
SfcI CTRYAG 2 cut(s) 611, 750
SinI GGWCC 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 9 cut(s) 441, 674, 824, 848, 867, 882, 894, 999, 1152
SseBI AGGCCT 2 cut(s) 547, 904
SsiI CCGC 3 cut(s) 15, 206, 811
SspI AATATT 1 cut(s) 601
StuI AGGCCT 2 cut(s) 547, 904
StyD4I CCNGG 3 cut(s) 139, 728, 775
StyI CCWWGG 2 cut(s) 157, 952
TaaI ACNGT 7 cut(s) 64, 335, 472, 499, 520, 568, 1087
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 87, 129
TasI AATT 9 cut(s) 441, 674, 824, 848, 867, 882, 894, 999, 1152
TatI WGTACW 1 cut(s) 736
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 6 cut(s) 414, 678, 866, 914, 1053, 1134
Tru9I TTAA 6 cut(s) 414, 678, 866, 914, 1053, 1134
TscAI CASTG 4 cut(s) 328, 502, 515, 615
TseFI GTSAC 2 cut(s) 58, 606
TseI GCWGC 2 cut(s) 613, 1010
Tsp45I GTSAC 2 cut(s) 58, 606
TspGWI ACGGA 1 cut(s) 48
TspRI CASTG 4 cut(s) 328, 502, 515, 615
VpaK11BI GGWCC 1 cut(s) 40
XapI RAATTY 3 cut(s) 674, 882, 999
XmiI GTMKAC 2 cut(s) 198, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.